CN106086216A - A kind of SNP marker associated with pteria martensii growth traits and primer and application - Google Patents
A kind of SNP marker associated with pteria martensii growth traits and primer and application Download PDFInfo
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- CN106086216A CN106086216A CN201610674361.3A CN201610674361A CN106086216A CN 106086216 A CN106086216 A CN 106086216A CN 201610674361 A CN201610674361 A CN 201610674361A CN 106086216 A CN106086216 A CN 106086216A
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Abstract
The invention discloses a kind of SNP marker associated with pteria martensii growth traits and primer and application.Detection primer includes: ASSN1184FI, ASSN1184RI, ASSN1184FO, ASSN1184RO.Utilize the detection primer of the SNP marker being associated with pteria martensii growth traits of the present invention, the SNP genotype site associated with pteria martensii growth traits can be amplified, growth vigor individuality can be filtered out intuitively further according to PCR amplification electrophoretogram and carry out breeding as standby parent, it is achieved molecular mark.
Description
Technical field:
The invention belongs to aquatic animal heredity and molecular mark technical field, be specifically related to a kind of and geneva pearl
The SNP marker of female shellfish growth traits association and primer and application.
Background technology:
Pteria martensii is China and the dominant species of sea water pearls cultivation in the world.China successfully carried out from nineteen sixty-five
Since pteria martensii artificial breeding, sea water pearls industry development is rapid, it has also become Guangdong, Guangxi and Hainan part littoral
One of district's sea-farming pillar industry.Nearly more than 10 years, quality and the yield of China's sea water pearls were decreased obviously, and international competitiveness subtracts
Weak, fanning economics glides.Trace it to its cause, in addition to breeding environment deterioration, cultural technique fall behind, an also major reason
It is: the most do not focus on selection-breeding and the reservation of kind of shellfish, lacks improved seeds；Seedling quality is uneven, and pinctada character is moved back
Change, small-sized, reduced capability etc. of growing cultured pearls as slow in growth, individual.For the healthy and stable development of China's sea water pearls aquaculture, cultivate
The pteria martensii improved seeds being suitable for China's sea area cultivation are extremely the most urgent.
Breeding technique has hybridization, selection and use, the multiple method such as biotechnology breeding and molecular breeding.Molecular breeding is mesh
Before the focus of research both at home and abroad, be the Main way of breeding technique development, it has efficiently, feature efficiently.Molecular breeding from
Not opening the exploitation of molecular marker, single nucleotide polymorphism (Single Nucleotide Polymorphism, SNP) is by gene
In group level, the variation of single base pair causes DNA sequence polymorphism (Sachidanandam et al., 2001), and it is the RFLP that continues
With the third generation molecular marker grown up after SSR molecular marker.The condition meeting SNP should be one of them allele
The frequency of the appearance in colony should be not less than 1% (Kang et al., 2002).This variation includes turning of single base
Change (exchange of homotype base), transversion (special-shaped base is exchanged), insert and lack, but the SNP of general indication does not include two kinds next
Situation.The SNP found at present, the overwhelming majority all only has 2 kinds of nucleotide, and the nucleotide of more than three kinds co-exists in a population
There's almost no, therefore SNP is two polymorphic alleles.SNP marker has the spies such as widely distributed, inheritance stability and dimorphism
Point, therefore the mechanism of causing a disease in human diseases achieves huge becoming in treatment, important crops and domestic animal genetic breeding
Effect.The most in recent years, high flux Roche454 sequencing technologies is utilized to obtain a large amount of SNP site and carry out and the associating of character
Analyze and find the report of new gene, show that SNP marker has become as hereditism and the main instrument of MAS.And at aquaculture
In animal, utilize SNP marker to carry out MAS breeding technique and be also in commitment, such as population genetic diversity analysis, kind mirror
The genetic breeding field aspects such as calmly, trait associations analysis, genetic linkage maps structure and QTL location.Appearing as of molecular marker
Realize genotype to select to provide possibility, because the genotype of molecular marker can identify.If target gene and certain
Molecular marker close linkage, then by the genotype of molecular marker is detected, it becomes possible to obtain the gene of target gene
Type.Therefore, it can by molecular marker, the genotype of objective trait be selected, here it is molecular marker assisted selection
(marker-assisted selection,MAS).Compared with conventional breeding methods, MAS has obvious superiority, especially
Be difficult to measure for those, heritability is relatively low and could show to obtain character in the later stage, and MAS is difficult to protected from environmental, does not has
Sex and the restriction at age, therefore, MAS can select in early days, shortens the generation inteval, and then improve the intensity selected and educate
The efficiency (Lande and Thompson, 1990) planted.And current, excavate the SNP mark associated with pteria martensii growth traits
Note seldom report, it is an object of the invention to provide a kind of new SNP marker associated with pteria martensii growth traits, it is provided that
A kind of method of this SNP of identification of living body, is beneficial to carry out genotype selection and use practice, makes genotype selection and use technology have
Actual operability.
Summary of the invention:
First purpose of the present invention is to provide the detection of a kind of SNP marker associated with pteria martensii growth traits and draws
Thing.
The detection primer of the SNP marker associated with pteria martensii growth traits of the present invention, it is characterised in that include with
Lower primer:
ASSN1184FI:5 '-CGATTTGCTAGAAAGCCATTA-3 '；
ASSN1184RI:5 '-AATTTGTTACAACTTACCTATAATGCAA-3 '；
ASSN1184FO:5 '-GGTAAAACATTAACACGGATTGA-3 '；
ASSN1184RO:5 '-ATTGTAATGACTTACCTAAAACGGAT-3 '.
Second object of the present invention is to provide a kind of SNP marker associated with pteria martensii growth traits, its feature
Being, the sequence of described SNP marker is as shown in SEQ ID NO.1, and total length is 1100bp, shown in described SEQ ID NO.1
Sequence the 457th bit base from 5 ' ends is A or T, represents this site with w in SEQ ID NO.1 sequence.
Shown in specific as follows:
GTGCATGTCCACTTGATGCGACCACTAAAACAGATGCAATAATTGCGAAAGATCGTGCCAAACTAAAATATTTCGAT
CGCAAAGTGGTCGTGCACCAAATGAGGAGGGGGCTTTAAAAATGTGATGGCATTTCAGAGAAATATAACTTGGGTTG
ATTTTGGTTAGATATGAGAAGGTAAAACATTAACACGGATTGATGGTATTTGGGACAGCTGAAAGTAGATATGAAAA
ATAAATACATGTACTGGGTTCGTAGTGAATAAAGTGGAGGGAGGGATATTCAGTACCCTTGTAGTTTGACGTCTTGC
TGTTTGACTGTGATGATGTTTTCATATTGTAGGAGATCGTGCTGGTAGTATTCTTTGGCCTGGAGTACGTAGTACGC
(W is A to TTATGGGCAGCAGGCTGTCGTAGTAAATACATTGGTGTTAGAGGACGTCTACGATT TGCTAGAAAGCCAATW
Or T) TCCATTATAGGTAAGTTGTAACAAATTTAAGGTGTTGACCGCCGCCGCCCCCTCCT CTGTCATATCTCTTATG
TCCGTCTCCCGATTCGTCTGTGTATTACAAAATGTTATAATCCCCACTAAGAGGCTCTAAATTTCTCCGACCGTTAG
AGAGCAAATATCCGTTTTAGGTAAGTCATTACAATTTTTTAGCTTAGCTTCCCTTCCCTACCTAGTCAATGCCTTGT
CCGTCTCTCATCTCGTCTGTGTATTATAGAAAACTGCTTTCTCCACACTGAGGCTCCCTATTTTCACCAACAGAGAG
TTAGAACAAATATGAGATAGCCCCCTTCCCTTCCTGTCCCATCTCTTGTCCGTCTTTCAGTTTGTCTGTGTATTACA
GAAAACTGCAACCTCCACTTGCATATGTAGAGGCTCCCAATTTTCACACACAGAGACACATGATTCGATATAAGAAT
CATTAACTCTTTATGTCTTTTACCTTTGCAAATTGTCGTTATTTACGTCTGCAGGTCGACCAGAGCGTCCCGTTACT
AGTAGATTAACTTTTTTTAGCAGTGTAAATAATCATGCGTGGAAAAAAGCGTCCTTACCATCTAAATGTCATCGTTA
AAGCATCGAATCAATACGGTACGGGAACCGT。
Third object of the present invention is to provide the SNP marker associated with pteria martensii growth traits at pteria martensii
Application in molecular mark.
Fourth object of the present invention is to provide a kind of pteria martensii that detects and has the individual method of growth vigor, its
It is characterised by, by the above-mentioned SNP marker associated with pteria martensii growth traits in pteria martensii to be measured is examined
Survey, determine whether described pteria martensii to be measured is the individuality with growth vigor, determine if to carry out as standby parent
Breeding.
Preferably, concretely comprise the following steps:
A, extract pteria martensii sample gene group DNA to be measured；
B, use above-mentioned detection primer ASSN1184FI, ASSN1184RI, ASSN1184FO, ASSN1184RO to geneva
Pinctada margaritifera sample gene group DNA carries out PCR amplification；
C, according to pcr amplification product, pteria martensii sample is carried out genotyping, when 310bp size 1 only occurs
Band for TT genotype, this pteria martensii sample is determined as the individuality with growth vigor, carries out breeding as standby parent.
Preferably, described PCR amplification, its PCR reaction system is: 25 μ L；PCR reaction system is as follows: comprise geneva pearl oyster
Shellfish sample gene group DNA 40ng, 1 × PCR Mixture, each 0.8 μM of detection primer ASSN1184FI, ASSN1184RI,
Each 0.2 μM of ASSN1184FO, ASSN1184RO, 0.1U Taq DNA enzymatic, surplus is water；Reaction condition is: 94 DEG C, 4min；94
DEG C 30s, 57 DEG C of 30s, 72 DEG C of 45s, 35 circulations；72 DEG C extend 10min.
Preferably, described pteria martensii sample gene group DNA to be measured of extracting is to extract pteria martensii sample to be measured
The genomic DNA of gill tissue.
5th purpose of the present invention is to provide a kind of SNP marker associated for detection with pteria martensii growth traits
Test kit, it is characterised in that include the detection primer of the above-mentioned SNP marker associated with pteria martensii growth traits.
Utilize the detection primer of the SNP marker being associated with pteria martensii growth traits of the present invention, it is possible to amplify
The SNP genotype site associated with pteria martensii growth traits, can filter out growth intuitively further according to PCR amplification electrophoretogram
Advantage individuality is used as standby parent and carries out breeding, it is achieved molecular mark.
Accompanying drawing illustrates:
Fig. 1 is the amplification electrophoretogram of the SNP marker associated with pteria martensii growth traits, wherein swimming lane 1-12 and swimming lane
14-25 is pteria martensii sample, and 13 is DL2000Maker, and band is followed successively by 2000bp, 1000bp, 750bp from top to bottom,
500bp, 250bp, 100bp.
Detailed description of the invention:
Following example are to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
Take 120 the pteria martensii individualities coming from the same group at random, after cleaning up, measure the growth of each shellfish
Character (shell height, shell length, shell width and body weight), opens shell with gag, and with shears clip, one piece of gill tissue is placed in 95% ethanol
In, in-20 DEG C of preservations, one_to_one corresponding record, the shellfish after sampling is continued cultivation.Use HiPure Universal DNA Kit
The genomic DNA of the gill tissue from 120 pteria martensiis is extracted by (Guangzhou Mei Ji biotech firm), and associative operation is joined
See that test kit description is carried out.After DNA extraction, the agarose gel electrophoresis in 1.0% detects its integrity, uv-spectrophotometric
The concentration of measurement amount DNA and purity, in-20 DEG C of preservations.
The detection primer of the SNP marker being associated with pteria martensii growth traits is:
ASSN1184FI:5 '-CGATTTGCTAGAAAGCCATTA-3 '；
ASSN1184RI:5 '-AATTTGTTACAACTTACCTATAATGCAA-3 '；
ASSN1184FO:5 '-GGTAAAACATTAACACGGATTGA-3 '；
ASSN1184RO:5 '-ATTGTAATGACTTACCTAAAACGGAT-3 '.
With above-mentioned primer ASSN1184FI, ASSN1184RI, ASSN1184FO, ASSN1184RO to pteria martensii sample
Genomic DNA expands at the enterprising performing PCR of PCR instrument.PCR reaction system is as follows: amplification system 25 μ L, comprises pteria martensii sample
Genomic DNA 0.5 μ L (40ng), 10 × PCR Mixture 2.5 μ L, 10 μMs of detection primers ASSN1184FI, ASSN1184RI
Each 2 μ L, 10 μMs of detection primers ASSN1184FO, each 0.5 μ L of ASSN1184RO, 0.1U Taq DNA enzymatic (Takara company), double
Steam water and complement to 25 μ L.Reaction condition is: 94 DEG C, 4min；94 DEG C of 30s, 57 DEG C of 30s, 72 DEG C of 45s, 35 circulations；72 DEG C of extensions
10min。
PCR primer 3% agarose (Guangzhou Kang Long science and technology biotech firm) gel electrophoresis, observes electricity with gel imaging system
Swimming collection of illustrative plates.Typing electrophoretogram as it is shown in figure 1, occur have 310bp and 206bp size 2 band for AT genotype (swimming lane 3,4,
6,8,9,10,11,12,15,17,18,23), only occur 310bp size 1 band for TT genotype (swimming lane 1,2,5,7,21,
22,24), only occur 206bp size 1 band for AA genotype (swimming lane 14,16,19,20,25).In these 120 individualities,
TT genotype individuals 22, shell height, shell length, shell width and body weight are respectively 62.39cm, 62.93cm, 23.88cm, 38.98g, AT
Genotype individuals 59, shell height, shell length, shell width and body weight are respectively 61.29cm, 62.78cm, 23.56cm, 37.22g, AA base
Because of individual 37 of type, shell height, shell length, shell width and body weight are respectively 58.80cm, 59.80cm, 23.23cm, 33.8g, have TT base
Because the individual growth traits value of type is maximum, the growth traits value of AT genotype individuals is taken second place, the character value of AA genotype individuals
Minimum.The general linear model in SPSS19.0 software (GLM) is utilized to be marked the correlation analysis with growth traits, gene
Difference Duancan between type correspondence character average carries out multiple comparisons analysis, the shell height of TT genotype individuals, shell length, shell
Wide and body weight is noticeably greater than AT genotype individuals and AA genotype individuals (P ＜ 0.05), i.e. thinks and has between them significantly
Significant difference.Beneficial gene type is TT, can pick out the individuality of TT genotype as standby parent, to cultivate superior progeny.
Claims (8)
1. the detection primer of the SNP marker associated with pteria martensii growth traits, it is characterised in that include following drawing
Thing:
ASSN1184FI:5 '-CGATTTGCTAGAAAGCCATTA-3 '；
ASSN1184RI:5 '-AATTTGTTACAACTTACCTATAATGCAA-3 '；
ASSN1184FO:5 '-GGTAAAACATTAACACGGATTGA-3 '；
ASSN1184RO:5 '-ATTGTAATGACTTACCTAAAACGGAT-3 '.
2. the SNP marker associated with pteria martensii growth traits, it is characterised in that the sequence of described SNP marker is such as
Shown in SEQ ID NO.1, the 457th bit base from 5 ' ends of sequence shown in described SEQ ID NO.1 is A or T.
3. the SNP marker associated with pteria martensii growth traits described in claim 2 is auxiliary at pteria martensii molecular marker
Help the application in breeding.
4. one kind is detected the method that pteria martensii has the individuality of growth vigor, it is characterised in that by geneva pearl to be measured
The SNP marker associated with pteria martensii growth traits described in claim 2 in female shellfish detects, and determines described to be measured
Whether pteria martensii is the individuality with growth vigor, determines if to carry out breeding as standby parent.
Method the most according to claim 4, it is characterised in that comprise the following steps:
A, extract pteria martensii sample gene group DNA to be measured；
B, use detection primer ASSN1184FI described in claim 1, ASSN1184RI, ASSN1184FO, ASSN1184RO
Pteria martensii sample gene group DNA is carried out PCR amplification；
C, according to pcr amplification product, pteria martensii sample is carried out genotyping, when 310bp size 1 band only occurs
For TT genotype, this pteria martensii sample is determined as the individuality with growth vigor, carries out breeding as standby parent.
Method the most according to claim 5, it is characterised in that described PCR amplification, its PCR reaction system is: 25 μ L；
PCR reaction system is as follows: comprise pteria martensii sample gene group DNA 40ng, 1 × PCR Mixture, detection primer
Each 0.8 μM of ASSN1184FI, ASSN1184RI, each 0.2 μM of ASSN1184FO, ASSN1184RO, 0.1U Taq DNA enzymatic, surplus
For water；Reaction condition is: 94 DEG C, 4min；94 DEG C of 30s, 57 DEG C of 30s, 72 DEG C of 45s, 35 circulations；72 DEG C extend 10min.
Method the most according to claim 5, it is characterised in that described extraction pteria martensii to be measured sample gene group
DNA is the genomic DNA of the gill tissue extracting pteria martensii sample to be measured.
8. the test kit of the SNP marker associated with pteria martensii growth traits for detection, it is characterised in that include power
Profit requires the detection primer of the SNP marker associated with pteria martensii growth traits described in 1.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108715896A (en) * | 2018-04-28 | 2018-10-30 | 中国科学院南海海洋研究所 | A method of screening the pteria martensii live body parent with good development character |
| CN110218801A (en) * | 2019-07-10 | 2019-09-10 | 广东海洋大学 | One kind EGFR gene SNP marker relevant to pteria martensii growth traits and its application |
| CN110218800A (en) * | 2019-07-10 | 2019-09-10 | 广东海洋大学 | One kind SNP marker relevant to pteria martensii growth traits and its application |
| CN111607652A (en) * | 2020-05-29 | 2020-09-01 | 中国科学院南海海洋研究所 | InDel marker associated with growth traits of pinctada martensii, detection primer group and development method |
| CN111676298A (en) * | 2020-07-09 | 2020-09-18 | 广东海洋大学 | Pinctada martensii growth trait related KSPI gene SNP molecular marker and application thereof |
| CN111705142A (en) * | 2020-07-09 | 2020-09-25 | 广东海洋大学 | MIP gene SNP molecular marker related to growth traits of pinctada martensii and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN104099415A (en) * | 2014-06-24 | 2014-10-15 | 中国科学院南海海洋研究所 | Detection primer marked by SNP and associated with pinctada martensi adductor muscle weight and application of detection primer |
| CN104711343A (en) * | 2014-12-31 | 2015-06-17 | 中国科学院南海海洋研究所 | SNP411871 marker associated to shell mould and weight of pinctada martensii, primer and application thereof |
-
2016
- 2016-08-16 CN CN201610674361.3A patent/CN106086216B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN104099415A (en) * | 2014-06-24 | 2014-10-15 | 中国科学院南海海洋研究所 | Detection primer marked by SNP and associated with pinctada martensi adductor muscle weight and application of detection primer |
| CN104711343A (en) * | 2014-12-31 | 2015-06-17 | 中国科学院南海海洋研究所 | SNP411871 marker associated to shell mould and weight of pinctada martensii, primer and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| YAOHUA SHI等: "High-density single nucleotide polymorphisms linkage and quantitative trait locus mapping of the pearl oyster, Pinctada fucata martensii Dunker", 《AQUACULTURE》 * |
| 李耀国等: "马氏珠母贝SNP标记开发及家系遗传多态性分析", 《海洋通报》 * |
| 韦国建等: "马氏珠母贝两个与生长性状相关QTL 的验证", 《海洋科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108715896A (en) * | 2018-04-28 | 2018-10-30 | 中国科学院南海海洋研究所 | A method of screening the pteria martensii live body parent with good development character |
| CN108715896B (en) * | 2018-04-28 | 2020-10-27 | 中国科学院南海海洋研究所 | Method for screening pinctada martensii living parent with excellent growth characteristics |
| CN110218801A (en) * | 2019-07-10 | 2019-09-10 | 广东海洋大学 | One kind EGFR gene SNP marker relevant to pteria martensii growth traits and its application |
| CN110218800A (en) * | 2019-07-10 | 2019-09-10 | 广东海洋大学 | One kind SNP marker relevant to pteria martensii growth traits and its application |
| CN111607652A (en) * | 2020-05-29 | 2020-09-01 | 中国科学院南海海洋研究所 | InDel marker associated with growth traits of pinctada martensii, detection primer group and development method |
| CN111676298A (en) * | 2020-07-09 | 2020-09-18 | 广东海洋大学 | Pinctada martensii growth trait related KSPI gene SNP molecular marker and application thereof |
| CN111705142A (en) * | 2020-07-09 | 2020-09-25 | 广东海洋大学 | MIP gene SNP molecular marker related to growth traits of pinctada martensii and application thereof |
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|---|---|
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