CN102057053A - 用于促红细胞生成素发酵生产的方法 - Google Patents

用于促红细胞生成素发酵生产的方法 Download PDF

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Publication number
CN102057053A
CN102057053A CN2009801207656A CN200980120765A CN102057053A CN 102057053 A CN102057053 A CN 102057053A CN 2009801207656 A CN2009801207656 A CN 2009801207656A CN 200980120765 A CN200980120765 A CN 200980120765A CN 102057053 A CN102057053 A CN 102057053A
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China
Prior art keywords
cell
erythropoietin
described method
cells
reactor
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Pending
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CN2009801207656A
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English (en)
Chinese (zh)
Inventor
W·维南德
F-R·孔茨
D·赖歇特
W·奥伊尔
R·汉科
C·比尔
M·辛霍费尔-沃夫拉
D·朔波尔-柯尼希
L·法贝尔
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Evonik Operations GmbH
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Evonik Degussa GmbH
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Publication of CN102057053A publication Critical patent/CN102057053A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2009801207656A 2008-06-04 2009-06-03 用于促红细胞生成素发酵生产的方法 Pending CN102057053A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102008002210.1 2008-06-04
DE102008002210A DE102008002210A1 (de) 2008-06-04 2008-06-04 Verfahren zur fermentativen Herstellung von Erythropoietin
PCT/EP2009/056820 WO2009147175A1 (de) 2008-06-04 2009-06-03 Verfahren zur fermentativen herstellung von erythropoietin

Publications (1)

Publication Number Publication Date
CN102057053A true CN102057053A (zh) 2011-05-11

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ID=41090241

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CN2009801207656A Pending CN102057053A (zh) 2008-06-04 2009-06-03 用于促红细胞生成素发酵生产的方法

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US (1) US20110189732A1 (enExample)
EP (1) EP2283147A1 (enExample)
JP (1) JP2011521660A (enExample)
CN (1) CN102057053A (enExample)
BR (1) BRPI0913622A2 (enExample)
CA (1) CA2727045A1 (enExample)
DE (1) DE102008002210A1 (enExample)
IL (1) IL209655A0 (enExample)
WO (1) WO2009147175A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103820514A (zh) * 2014-03-10 2014-05-28 深圳赛保尔生物药业有限公司 一种高效生产促红细胞生成素的方法

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
US9421553B2 (en) 2010-08-23 2016-08-23 Flodesign Sonics, Inc. High-volume fast separation of multi-phase components in fluid suspensions
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9688958B2 (en) 2012-03-15 2017-06-27 Flodesign Sonics, Inc. Acoustic bioreactor processes
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US9422328B2 (en) 2012-03-15 2016-08-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US11324873B2 (en) 2012-04-20 2022-05-10 Flodesign Sonics, Inc. Acoustic blood separation processes and devices
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
WO2015006730A1 (en) * 2013-07-12 2015-01-15 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
CN111948015B (zh) 2014-10-24 2023-10-10 生命科技股份有限公司 以声学方式沉淀的液-液样品纯化系统
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
CA2984492A1 (en) 2015-04-29 2016-11-03 Flodesign Sonics, Inc. Acoustophoretic device for angled wave particle deflection
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US10640760B2 (en) 2016-05-03 2020-05-05 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
RU2708048C2 (ru) 2015-05-20 2019-12-03 Флодизайн Соникс, Инк. Способ акустического манипулирования частицами в полях стоячих волн
WO2016201385A2 (en) 2015-06-11 2016-12-15 Flodesign Sonics, Inc. Acoustic methods for separation cells and pathogens
US9663756B1 (en) 2016-02-25 2017-05-30 Flodesign Sonics, Inc. Acoustic separation of cellular supporting materials from cultured cells
CN112044720B (zh) 2015-07-09 2023-07-04 弗洛设计声能学公司 非平面和非对称压电晶体及反射器
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US10710006B2 (en) 2016-04-25 2020-07-14 Flodesign Sonics, Inc. Piezoelectric transducer for generation of an acoustic standing wave
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
JP2020513248A (ja) 2016-10-19 2020-05-14 フロデザイン ソニックス， インク．Ｆｌｏｄｅｓｉｇｎ Ｓｏｎｉｃｓ， Ｉｎｃ． 音響による親和性細胞抽出
US10785574B2 (en) 2017-12-14 2020-09-22 Flodesign Sonics, Inc. Acoustic transducer driver and controller

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008036600A2 (en) * 2006-09-18 2008-03-27 Genentech, Inc. Methods of protein production

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
IL77081A (en) 1984-12-04 1999-10-28 Genetics Inst Dna sequence encoding human erythropoietin process for the preparation thereof and a pharmaceutical composition of human erythropoietin
US4677195A (en) * 1985-01-11 1987-06-30 Genetics Institute, Inc. Method for the purification of erythropoietin and erythropoietin compositions
DE3787805T2 (de) 1986-08-04 1994-02-10 Garvan Inst Med Res Serumfreies gewebekulturmedium, das ein polymerzellenschutzmittel enthält.
US4954437A (en) * 1986-09-15 1990-09-04 Integrated Genetics, Inc. Cell encoding recombinant human erythropoietin
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
BR9105208A (pt) 1990-11-30 1992-07-21 Ajinomoto Kk Processo para o cultivo aerobico de um microorganismo em uma cultura alimentada em batelada,cultura continua ou cultura continua com reciclagem de celulas,aparelho para controlar a concentracao da fonte de carbono do substrato e processo para produzir l-lisina por fermentacao
US5626767A (en) 1993-07-02 1997-05-06 Sonosep Biotech Inc. Acoustic filter for separating and recycling suspended particles
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
IL118201A (en) * 1995-05-11 2004-12-15 Roche Diagnostics Gmbh Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof
PT889949E (pt) 1996-03-13 2003-10-31 Delta Biotechnology Ltd Controlo de fermentacao
DK1036179T4 (da) * 1997-12-03 2014-03-31 Roche Diagnostics Gmbh Fremgangsmåde til fremstilling af polypeptider med egnet glycosilering
BR9917606A (pt) * 1998-11-06 2002-12-31 Bio Sidus S A Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento
US20020099183A1 (en) 2000-08-23 2002-07-25 Pluschkell Stefanie Beate Process for the preparation of neutrophil inhibitory factor
DE10255508A1 (de) * 2002-11-27 2004-06-17 Forschungszentrum Jülich GmbH Verfahren zur Kultivierung von Zellen zur Produktion von Substanzen
DE102004027816A1 (de) * 2004-06-08 2006-01-05 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von Erythropoietin
AU2006322028C1 (en) * 2005-12-08 2025-09-04 Amgen Inc. Improved host cells and culture methods
DE102007042600A1 (de) * 2007-09-07 2009-03-12 Evonik Degussa Gmbh Verfahren zur Herstellung von enantiomerenangereichten Aminen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008036600A2 (en) * 2006-09-18 2008-03-27 Genentech, Inc. Methods of protein production

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
WANG MD ET AL.: "Erythropoietin production from CHO cells grown by continuous culture in a fluidized-bed bioreactor", 《BIOTECHNOL BIOENG》 *
张映辉等: "人促红细胞生成素受体胞外区基因的克隆及其在大肠杆菌中的表达", 《生物工程学报》 *
林珍等: "人促红细胞生成素检测方法研究进展", 《生命科学仪器》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103820514A (zh) * 2014-03-10 2014-05-28 深圳赛保尔生物药业有限公司 一种高效生产促红细胞生成素的方法
CN103820514B (zh) * 2014-03-10 2017-10-31 深圳赛保尔生物药业有限公司 一种高效生产促红细胞生成素的方法

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Publication number Publication date
EP2283147A1 (de) 2011-02-16
JP2011521660A (ja) 2011-07-28
US20110189732A1 (en) 2011-08-04
CA2727045A1 (en) 2009-12-10
DE102008002210A1 (de) 2009-12-10
BRPI0913622A2 (pt) 2015-08-25
WO2009147175A1 (de) 2009-12-10
IL209655A0 (en) 2011-02-28

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Application publication date: 20110511