CN102057042B - 家族44木葡聚糖酶变体 - Google Patents

家族44木葡聚糖酶变体 Download PDF

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Publication number
CN102057042B
CN102057042B CN200980121086.0A CN200980121086A CN102057042B CN 102057042 B CN102057042 B CN 102057042B CN 200980121086 A CN200980121086 A CN 200980121086A CN 102057042 B CN102057042 B CN 102057042B
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Prior art keywords
xyloglucanase
variant
seq
parent
polypeptide
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CN200980121086.0A
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English (en)
Chinese (zh)
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CN102057042A (zh
Inventor
沃纳·贝森马特
艾斯本·P·弗里伊斯
基思·吉布森
弗兰克·W·拉斯马森
迈克尔·斯克乔特
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Novozymes AS
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Novozymes AS
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Priority to CN201410265667.4A priority Critical patent/CN104017789B/zh
Publication of CN102057042A publication Critical patent/CN102057042A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01151—Xyloglucan-specific endo-beta-1,4-glucanase (3.2.1.151), i.e. endoxyloglucanase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN200980121086.0A 2008-06-06 2009-06-04 家族44木葡聚糖酶变体 Active CN102057042B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410265667.4A CN104017789B (zh) 2008-06-06 2009-06-04 家族44木葡聚糖酶变体

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08157769 2008-06-06
EP08157769.4 2008-06-06
PCT/EP2009/056875 WO2009147210A1 (en) 2008-06-06 2009-06-04 Variants of a family 44 xyloglucanase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201410265667.4A Division CN104017789B (zh) 2008-06-06 2009-06-04 家族44木葡聚糖酶变体

Publications (2)

Publication Number Publication Date
CN102057042A CN102057042A (zh) 2011-05-11
CN102057042B true CN102057042B (zh) 2014-07-16

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN200980121086.0A Active CN102057042B (zh) 2008-06-06 2009-06-04 家族44木葡聚糖酶变体
CN201410265667.4A Active CN104017789B (zh) 2008-06-06 2009-06-04 家族44木葡聚糖酶变体

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CN201410265667.4A Active CN104017789B (zh) 2008-06-06 2009-06-04 家族44木葡聚糖酶变体

Country Status (9)

Country Link
US (3) US8709777B2 (https=)
EP (3) EP2905333B1 (https=)
JP (1) JP2011521661A (https=)
CN (2) CN102057042B (https=)
AU (1) AU2009254546B2 (https=)
CA (1) CA2727005A1 (https=)
MX (1) MX2010013183A (https=)
RU (1) RU2545721C2 (https=)
WO (1) WO2009147210A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103232984B (zh) * 2013-04-10 2014-08-27 中国科学院南海海洋研究所 一种新颖的糖苷水解酶家族44的纤维素酶及其编码基因和应用
CN104388406B (zh) * 2014-10-29 2017-02-15 广西大学 一种内切木葡聚糖酶及其应用
US20190257031A1 (en) * 2015-10-23 2019-08-22 Novozymes A/S Use of Cellulase to improve Viscosity Control of Dissolving Pulp
EP4204551B1 (en) * 2020-08-25 2025-09-17 Novozymes A/S Variants of a family 44 xyloglucanase
US20250179393A1 (en) 2022-03-02 2025-06-05 Novozymes A/S Use of xyloglucanase for improvement of sustainability of detergents
WO2023247348A1 (en) 2022-06-21 2023-12-28 Novozymes A/S Mannanase variants and polynucleotides encoding same
EP4461796A1 (en) 2023-05-10 2024-11-13 Novozymes A/S Detergent composition comprising laccase
EP4461795A1 (en) 2023-05-10 2024-11-13 Novozymes A/S Detergent composition comprising laccase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1230987A (zh) * 1996-09-17 1999-10-06 诺沃挪第克公司 纤维素酶变体
CN1272880A (zh) * 1997-07-11 2000-11-08 加拿大农业及农业食品部 具有1,3和1,4－β－葡聚糖酶活性的小盾壳霉菌株
EP1136892A1 (fr) * 2000-03-20 2001-09-26 Audemars Piguet (Renaud et Papi) SA Mécanisme d'entraínement d'un cliquet de retenue pour rochet de remontoir d'une pièce d'horlogerie

Family Cites Families (19)

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DK16490D0 (da) * 1990-01-19 1990-01-19 Novo Nordisk As Enzym
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DK0675951T3 (da) 1992-12-23 2003-09-08 Novozymes As Enzym med endoglucanaseaktivitet
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
ATE429490T1 (de) 1995-05-05 2009-05-15 Novozymes As Protease-varianten und verbindungen
WO1999002663A1 (en) 1997-07-07 1999-01-21 Novo Nordisk A/S Alkaline xyloglucanase
EP0905306A1 (de) * 1997-09-26 1999-03-31 Consortium für elektrochemische Industrie GmbH Mehrkomponentensystem zum Verändern, Abbau oder Bleichen von Lignin, ligninhaltigen Materialien sowie Verfahren zu seiner Anwendung
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6815192B2 (en) * 2000-02-24 2004-11-09 Novozymes A/S Family 44 xyloglucanases
ES2322690T3 (es) 2000-02-24 2009-06-25 Novozymes A/S Xiloglucanasas de la familia 44.
WO2003012036A2 (en) 2001-07-27 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
AU2003223928A1 (en) 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
US20080015135A1 (en) 2006-05-05 2008-01-17 De Buzzaccarini Francesco Compact fluid laundry detergent composition
MX2008016448A (es) 2006-06-19 2009-01-22 Procter & Gamble Composiciones detergentes liquidas con polimeros a base de acido poliacrilico y polidispesidad baja.
PL3404088T3 (pl) 2008-06-06 2025-04-28 The Procter & Gamble Company Kompozycja detergentu zawierająca odmianę z grupy 44 ksyloglukanazy

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1230987A (zh) * 1996-09-17 1999-10-06 诺沃挪第克公司 纤维素酶变体
CN1272880A (zh) * 1997-07-11 2000-11-08 加拿大农业及农业食品部 具有1,3和1,4－β－葡聚糖酶活性的小盾壳霉菌株
EP1136892A1 (fr) * 2000-03-20 2001-09-26 Audemars Piguet (Renaud et Papi) SA Mécanisme d'entraínement d'un cliquet de retenue pour rochet de remontoir d'une pièce d'horlogerie

Also Published As

Publication number Publication date
US20160333330A1 (en) 2016-11-17
CN102057042A (zh) 2011-05-11
US9382524B2 (en) 2016-07-05
EP2905333A1 (en) 2015-08-12
RU2010154132A (ru) 2012-07-20
CN104017789B (zh) 2017-11-03
US10035998B2 (en) 2018-07-31
AU2009254546B2 (en) 2014-09-25
RU2545721C2 (ru) 2015-04-10
US8709777B2 (en) 2014-04-29
CA2727005A1 (en) 2009-12-10
AU2009254546A1 (en) 2009-12-10
WO2009147210A1 (en) 2009-12-10
CN104017789A (zh) 2014-09-03
US20140302586A1 (en) 2014-10-09
EP2905333B1 (en) 2017-08-09
EP2674488A1 (en) 2013-12-18
EP2288697A1 (en) 2011-03-02
EP2288697B1 (en) 2013-11-06
US20110092409A1 (en) 2011-04-21
JP2011521661A (ja) 2011-07-28
MX2010013183A (es) 2011-01-21

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