CN102028096A - Production method of tray liquid high-density bacterial proteins - Google Patents
Production method of tray liquid high-density bacterial proteins Download PDFInfo
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- CN102028096A CN102028096A CN2009101796205A CN200910179620A CN102028096A CN 102028096 A CN102028096 A CN 102028096A CN 2009101796205 A CN2009101796205 A CN 2009101796205A CN 200910179620 A CN200910179620 A CN 200910179620A CN 102028096 A CN102028096 A CN 102028096A
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Abstract
The invention discloses a production method of tray liquid high-density bacterial proteins. In the method, a culture medium taking apple residues as basic raw materials is utilized, so that a bacterial protein feed with the protein content of more than 30 percent can be obtained. The method comprises the following steps of: (1) preparing a composite fungicide, namely, inoculating aspergillus niger CCTCC NO:M209125 onto a bran culture medium according to the inoculum size of between 50 and 100 mL/kg, culturing for 2 to 5 days and drying a culture in the air; inoculating candida tropicalis and beer yeast onto a corn meal culture medium, mixing and culturing at constant temperature for 2 to 5 days, drying a culture in the air and mixing the two cultures so as to obtain the composite fungicide; (2) preparing raw materials, namely, preparing 5 to 10 percent of corn meal to obtain a liquid culture medium by using tap water; (3) inoculating; (4) charging; and (5) culturing, namely, culturing the liquid culture medium into which the composite fungicide is added in a tray at the temperature of between 20 and 40 DEG C, wherein the thickness of a pellicle reaches 2 centimeters 2 to 7 days later.
Description
Technical field
The invention belongs to the agricultural byproducts reutilization technology in the circular agriculture, relating to the corn flour is raw material obtains mycoprotein by liquid High Density Cultivation means method.
Background technology
Through studies show that in a large number of relevant scholar: the space mutagenesis Microbial Breeding has advantages such as variation efficient height, amplitude are big, easily stable, optimum variation is many; The space particular surroundings can improve the frequency of mutation of some gene in the microorganism significantly, and spatial manipulation can become the effective way that obtains the microorganism excellent species.Utilize space treatment seed selection new crop varieties, agriculturally use comparatively widely, but utilize space technology to cultivate new bacterial strain, and be used for the synthetic of mycoprotein, particularly under the liquid condition, do not appear in the newspapers as yet by the technology of liquid High Density Cultivation raising protein synthetic ratio.
Summary of the invention
Technical problem to be solved by this invention provides a kind of tray liquid high density mycoprotein production method, and it is that to utilize with the pomace be the culture medium of base stock, can obtain protein content and reach animal feeding-stuff containing somatic protein more than 30%.
For solving the problems of the technologies described above, the present invention adopts the basic design of technical scheme to be: it comprises the following steps:
(1) preparation composite bacteria agent capable
At first the preserving number that space breeding is obtained be aspergillus niger ZM-8 (the Aspergillus niger ZM-8) bacterial strain of CCTC NO:M209125 and produce in common candida tropicalis and brewer's yeast make 1.0 * 10 with distilled water respectively
11～5.0 * 10
11The liquid spawn of individual spore or cell/L, described aspergillus niger CCTC NO:M209125 is inoculated on the bran mass by the inoculum concentration of 50～100mL/kg, in 20 ℃～35 ℃ constant temperature culture after 2 days～5 days, air-dry culture; Candida tropicalis and brewer's yeast all are inoculated on the maize powder medium by the inoculum concentration of 50～100mL/kg, 20 ℃～35 ℃ constant temperature Mixed culture are after 2～5 days, air-dry culture, be the two culture 2～5 by mass ratio at last: 1 is mixed into composite bacteria agent capable; Wherein, aspergillus niger ZM-8 (Aspergillus niger ZM-8) bacterial strain, by the depositary institution's preservation that is defined in State Intellectual Property Office approval of the 25 of Patent Law detailed rules for the implementation, preservation date: on June 10th, 2009; Depositary institution's title and abbreviation: Chinese typical culture collection center, CCTCC; Deposit number: CCTCCNO:M209125.
Wherein, described bran mass is pressed the percentage of quality kg/ volume L, for: wheat bran 4%～8%, pomace 2%～6%, ammonium phosphate 0.5%～2%, magnesium sulfate 0.01%～0.05%, potassium dihydrogen phosphate 0.002～0.012%, with the running water preparation, the pH value is 4.0～7.0.
Wherein, described maize powder medium is pressed the percentage of quality kg/ volume L, for: cornstarch 3%～7%, potassium dihydrogen phosphate 0.05%～0.2%, with the running water preparation, the pH value is 4.0～7.0.
Wherein, described bran mass or maize powder medium were sterilized 15 minutes～30 minutes down for 121 ℃ in temperature.
(2) raw material preparation
By the percentage of quality kg/ volume L, corn flour 5%～10%, ammonium sulfate 1%～5%, white sugar 1%～5%, gibberellin 2ppm～8ppm, potassium dihydrogen phosphate 0.5%～1.0%, be mixed with fluid nutrient medium with running water, the pH value was 4.0～7.0,121 ℃ of sterilizations 15 minutes～30 minutes.
(3) inoculation
Under the condition of 20～35 ℃ of product temperature, add 0.5%～1% described composite bacteria agent capable in fluid nutrient medium by the percentage of quality kg/ volume L, stir evenly.
(4) charging
Described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray.
Wherein, the described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray of 6L～10L, every dish dress 5kg～8kg nutrient solution covers with preservative film.
(5) cultivate
Described fluid nutrient medium behind the described composite bacteria agent capable of adding in the tray put under 20 ℃～40 ℃ conditions cultivate, the thickness of mycoderm can reach 2cm after 2 days～7 days, and wherein cultivating the tray degree of depth is 3cm, during should note down dish.
Also comprise the following steps: behind the described incubation step
(1) filters
The mycoderm compound that ferments is filtered in filter, obtain wet thallus.
(2) oven dry
Wet thallus dried by the fire in 120 ℃ of dryers after 2 hours, and cooling is pulverized, and quality inspection directly joins in the feed to scale.
The invention has the beneficial effects as follows, utilize the cellulose degradation strain excellent aspergillus niger CCTC NO:M209125 that space flight obtains and produce in brewer's yeast, candida tropicalis commonly used mix the composite microbial inoculum of making, and by liquid High Density Cultivation mycoprotein, the content of mycoprotein amplifying nucleic acid is low than bacterium, and is favourable to the growth of domestic animal; So that inorganic nitrogen---the sulphur ammonium is main nitrogen, can reduce production costs significantly widely; Vitamin content in the mycothallus albumen is higher; Every 1L fluid nutrient medium can obtain wet thallus 400g, after the oven dry, can get the 50g dry mycelium, and crude protein content reaches 49%; Simple to operate, equipment drops into little; Production scale by the dried mycothallus albumen of producing 1000kg daily is calculated, and about 300,000 yuan of equipment investment comprises mixer, digester, sweathouse, culture plate, drying baker and pulverizer etc., but does not comprise the factory building investment; Production cost is low, about 1000 yuan of the cost of material of every 1000kg dry mycelium.
Aspergillus niger ZM-8 (Aspergillus niger ZM-8) bacterial strain, by the depositary institution's preservation that is defined in State Intellectual Property Office approval of the 25 of Patent Law detailed rules for the implementation, preservation date: on June 10th, 2009; Depositary institution's title and abbreviation: Chinese typical culture collection center, CCTCC; Deposit number: CCTCCNO:M209125.
The specific embodiment
It comprises the following steps:
(1) preparation composite bacteria agent capable
Common candida tropicalis and brewer's yeast makes 1.0 * 10 with distilled water respectively in aspergillus niger CCTCC NO:M209125 bacterial strain that at first space breeding is obtained and the production
11～5.0 * 10
11The liquid spawn of individual spore or cell/L, aspergillus niger CCTCC NO:M209125 is inoculated on the bran mass by the inoculum concentration of 50～100mL/kg, in 20 ℃～35 ℃ constant temperature culture after 2～5 days, air-dry culture; Candida tropicalis and brewer's yeast all are inoculated on the maize powder medium by the inoculum concentration of 50～100mL/kg, 20 ℃～35 ℃ constant temperature Mixed culture are after 2～5 days, air-dry culture, be the two culture 2～5 by mass ratio at last: 1 is mixed into composite bacteria agent capable.
Wherein, described bran mass is pressed the percentage of quality kg/ volume L, for: wheat bran 4%～8%, pomace 2%～6%, ammonium phosphate 0.5%～2%, magnesium sulfate 0.01%～0.05%, potassium dihydrogen phosphate 0.002%～0.012%, with the running water preparation, the pH value is 4.0～7.0.
Wherein, described maize powder medium is pressed the percentage of quality kg/ volume L, for: cornstarch 3%～7%, potassium dihydrogen phosphate 0.05%～0.2%, with the running water preparation, the pH value is 4.0～7.0.
Wherein, described maize powder medium or bran mass were sterilized 15 minutes～30 minutes down for 121 ℃ in temperature.
(2) raw material preparation
By the percentage of quality kg/ volume L, corn flour 5%～10%, ammonium sulfate 1%～5%, white sugar 1%～5%, gibberellin 2ppm～8ppm, potassium dihydrogen phosphate 0.5%～1.0%, be mixed with fluid nutrient medium with running water, the pH value was 4.0～7.0,121 ℃ of sterilizations 15 minutes～30 minutes.
(3) inoculation
Under the condition of 20 ℃～35 ℃ of product temperature, add 0.5%～1% described composite bacteria agent capable in fluid nutrient medium by the percentage of quality kg/ volume L, stir evenly.
(4) charging
Described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray.
Wherein, the described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray of 6L～10L, every dish dress 5kg～8kg nutrient solution covers with preservative film.
(5) cultivate
Described fluid nutrient medium behind the described composite bacteria agent capable of adding in the tray put under 20 ℃～40 ℃ conditions cultivate, the thickness of mycoderm can reach 2cm after 2 days～7 days, and wherein cultivating the tray degree of depth is 3cm, during should note down dish.
Also comprise the following steps: behind the described incubation step
(1) filters
The mycoderm compound that ferments is filtered in filter, obtain wet thallus.
(2) oven dry
Wet thallus dried by the fire in 120 ℃ of dryers after 2 hours, and cooling is pulverized, and quality inspection directly joins in the feed to scale.
Claims (6)
1. a tray liquid high density mycoprotein production method is characterized in that it comprises the following steps:
(1) preparation composite bacteria agent capable
Common candida tropicalis and brewer's yeast makes 1.0 * 10 with distilled water respectively in aspergillus niger ZM-8 bacterial classification that at first space breeding is obtained and the production
11～5.0 * 10
11The liquid spawn of individual spore or cell/L, aspergillus niger ZM-8 is inoculated on the bran mass by the inoculum concentration of 50～100mL/kg, in 20 ℃～35 ℃ constant temperature culture after 2 days～5 days, air-dry culture; Candida tropicalis and brewer's yeast all are inoculated on the maize powder medium by the inoculum concentration of 50～100mL/kg, 20 ℃～35 ℃ constant temperature Mixed culture are after 2 days～5 days, air-dry culture, be the two culture 2～5 by mass ratio at last: 1 is mixed into composite bacteria agent capable.
(2) raw material preparation
By the percentage of quality kg/ volume L, for: corn flour 5%～10%, ammonium sulfate 1%～5%, white sugar 1%～5%, gibberellin 2ppm～8ppm, potassium dihydrogen phosphate 0.5%～1.0%, be mixed with fluid nutrient medium with running water, the pH value was 4.0～7.0,121 ℃ of sterilizations 15 minutes～30 minutes.
(3) inoculation
Under the condition of 20 ℃～35 ℃ of product temperature, add 0.5%～1% described composite bacteria agent capable in fluid nutrient medium by the percentage of quality kg/ volume L, stir evenly.
(4) charging
Described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray.
(5) cultivate
Described fluid nutrient medium behind the described composite bacteria agent capable of adding in the tray put under 20 ℃～40 ℃ conditions cultivate, the thickness of mycoderm can reach 2cm after 2 days～7 days, and wherein cultivating the tray degree of depth is 3cm, during should note down dish.
2. tray liquid high density mycoprotein production method according to claim 1, it is characterized in that, described bran mass is by the percentage of quality kg/ volume L, for: wheat bran 4%～8%, pomace 2%～6%, ammonium phosphate 0.5%～2%, magnesium sulfate 0.01%～0.05%, potassium dihydrogen phosphate 0.002%～0.012%, with the running water preparation, the pH value is 4.0～7.0.
3. tray liquid high density mycoprotein production method according to claim 1 is characterized in that, described maize powder medium is by the percentage of quality kg/ volume L, for: cornstarch 3%～7%, potassium dihydrogen phosphate 0.05%～0.2%, with the running water preparation, the pH value is 4.0～7.0.
4. according to claim 1 or 2 or 3 described tray liquid high density mycoprotein production methods, it is characterized in that described maize powder medium or bran mass were sterilized 15 minutes～30 minutes down for 121 ℃ in temperature.
5. tray liquid high density mycoprotein production method according to claim 1, it is characterized in that, in the described charging step, the described fluid nutrient medium branch behind the described composite bacteria agent capable of adding is installed in the tray of 6L～10L, every dish dress 5kg～8kg nutrient solution covers with preservative film.
6. tray liquid high density mycoprotein production method according to claim 1 is characterized in that it also comprises the following steps:
(1) filters
The mycoderm compound that ferments is filtered in filter, obtain wet thallus.
(2) oven dry
Wet thallus dried by the fire in 120 ℃ of dryers after 2 hours, and cooling is pulverized, and quality inspection directly joins in the feed to scale.
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| CN2009101796205A CN102028096A (en) | 2009-10-06 | 2009-10-06 | Production method of tray liquid high-density bacterial proteins |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757255A (en) * | 2015-03-17 | 2015-07-08 | 陕西科技大学 | Method for preparing high-protein pomace by utilizing fresh pomace by submerged fermentation technology |
| CN114621908A (en) * | 2022-03-16 | 2022-06-14 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
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| CN1283399A (en) * | 2000-07-19 | 2001-02-14 | 陈五岭 | Process for preparing protein feed by solid fermantation of fruit dregs |
| CN1470181A (en) * | 2003-04-24 | 2004-01-28 | 西北农林科技大学 | Method for producing protein feed by using apple residue double-baterial fermentation |
| CN1504102A (en) * | 2002-11-29 | 2004-06-16 | 上海九川科技发展有限公司 | Process of fodder with bioactivity |
| CN1663425A (en) * | 2005-03-30 | 2005-09-07 | 南京大学 | Method for fermenting solid-state aquatic plant by microbe to produce protein feedstuff |
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2009
- 2009-10-06 CN CN2009101796205A patent/CN102028096A/en active Pending
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| US3775393A (en) * | 1970-12-03 | 1973-11-27 | Standard Oil Co | Ammonia extraction of unicellular microorganisms |
| US3784536A (en) * | 1971-02-04 | 1974-01-08 | Standard Oil Co | Process for reducing the nucleic acid content of single cell protein affording microorganisms |
| CN1283399A (en) * | 2000-07-19 | 2001-02-14 | 陈五岭 | Process for preparing protein feed by solid fermantation of fruit dregs |
| CN1504102A (en) * | 2002-11-29 | 2004-06-16 | 上海九川科技发展有限公司 | Process of fodder with bioactivity |
| CN1470181A (en) * | 2003-04-24 | 2004-01-28 | 西北农林科技大学 | Method for producing protein feed by using apple residue double-baterial fermentation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757255A (en) * | 2015-03-17 | 2015-07-08 | 陕西科技大学 | Method for preparing high-protein pomace by utilizing fresh pomace by submerged fermentation technology |
| CN114621908A (en) * | 2022-03-16 | 2022-06-14 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
| CN114621908B (en) * | 2022-03-16 | 2024-03-22 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
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Application publication date: 20110427 |