CN102021120A - Paecilonyces variotii strain and application thereof - Google Patents
Paecilonyces variotii strain and application thereof Download PDFInfo
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- CN102021120A CN102021120A CN 201010253652 CN201010253652A CN102021120A CN 102021120 A CN102021120 A CN 102021120A CN 201010253652 CN201010253652 CN 201010253652 CN 201010253652 A CN201010253652 A CN 201010253652A CN 102021120 A CN102021120 A CN 102021120A
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- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 223
- 230000015556 catabolic process Effects 0.000 claims abstract description 48
- 238000006731 degradation reaction Methods 0.000 claims abstract description 48
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 claims description 21
- 241000079253 Byssochlamys spectabilis Species 0.000 claims description 20
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 21
- 229920001817 Agar Polymers 0.000 description 11
- 239000008272 agar Substances 0.000 description 11
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 10
- 239000002689 soil Substances 0.000 description 8
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 6
- 235000011613 Pinus brutia Nutrition 0.000 description 6
- 241000018646 Pinus brutia Species 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 241000589308 Methylobacterium extorquens Species 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 235000007466 Corylus avellana Nutrition 0.000 description 2
- 240000003211 Corylus maxima Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000237502 Ostreidae Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 241000589630 Pseudomonas pseudoalcaligenes Species 0.000 description 2
- 241000589776 Pseudomonas putida Species 0.000 description 2
- 244000007853 Sarothamnus scoparius Species 0.000 description 2
- 235000019219 chocolate Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000000416 exudates and transudate Anatomy 0.000 description 2
- 239000008098 formaldehyde solution Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 235000020636 oyster Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241001653918 Halomonas sp. Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241001136550 Penicillium javanicum Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000168225 Pseudomonas alcaligenes Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003413 degradative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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Abstract
The invention discloses a paecilonyces variotii strain H6 and application thereof. The paecilonyces variotii strain H6 with a preservation number of CGMCC No. 3722 is preserved at China General Microbiological Culture Collection Center on April 9, 2010. The paecilonyces variotii strain H6 widely spreads in the nature, quickly propagates and has strong growth capability. The invention provides the application of the paecilonyces variotii strain H6 in formaldehyde degradation and provides the technical support for the biological treatment engineering with formaldehyde pollution.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of Degradation Formaldehyde bacterium, be specifically related to a kind of paecilomyces varioti H6 (Paecilomyces variotii) and the application aspect degradation of formaldehyde thereof.
Background technology
In response to the field extensively and importance, about the Degradation Formaldehyde Research on ability of Degradation Formaldehyde bacterium report is arranged early.
Early stage people get down to the research of lower concentration degradation of formaldehyde, and report is arranged during the high density degradation of formaldehyde in recent years.The isolating Pseudomonas putida of Adroer etc. (1990) A2 can degradation of formaldehyde 250mgL
-1The isolating Halomonas sp.MAC of Azachi1 etc. (1995) only can tolerate formaldehyde 75～100mgL
-1The isolating Pseudomonas.alcaligenes of Doronina etc. (1997) can degradation of formaldehyde 200mgL
-1, strain Pseudomonas putida J3 can degradation of formaldehyde 450mgL
-1, Methylobacterium extorquens can degradation of formaldehyde 500～1000mgL
-1One strain Pseudomonas alcaligenes cultivates can degradation of formaldehyde 2000mgL after 3 days
-1(NSW OSS) can degradation of formaldehyde 1850mgL for LSW, SSW for Mirdamadi etc. (2005) report Methylobacterium extorquens (ESS and PSS) and four strain Pseudomonas pseudoalcaligenes
-1, wherein strain Pseudomonas pseudoalcaligenes OSS cultivates 24h and consumes 3700mgL
-1Formaldehyde 100% is cultivated 72h and is consumed 5920mgL
-1Formaldehyde 70%, Methylobacterium extorquens ESS and Methylobacterium extorquens PSS be degradation of formaldehyde 2960mgL fully
-1Bonastre etc. (1986) are reported in C
FormaldehydeBe 2300mgdm
-3Active sludge do in the experiment of matrix can part degradation of formaldehyde, formaldehyde can be degraded by aerobic bacteria under aerobic condition; Degradation of formaldehyde 400mgdm when being carbon source with formaldehyde
-3, complete degradation of formaldehyde 2300mgdm in the wastewater treatment of active sludge factory
-3With phenol 2400mgdm
-3(Zagornaya et al, 1990).Yamazaki etc. (2001) have separated a strain formaldehyde resistant bacteria in seawater, find that it can degradation of formaldehyde 400mgdm in 3% sodium-chlor
-3, in the model of design such as Eiroa (2004), point out that nitrification can degradation of formaldehyde, when be carbon source with formaldehyde, when methyl alcohol is the association carbon source, the concentration that nitrobacteria can degradation of formaldehyde is 30～3890mgdm
-3, 1360mgdm and denitrifying bacterium can be degraded when having formaldehyde and methyl alcohol to exist
-3Formaldehyde (Eiroa et al, 2006).
No matter existing research is lower concentration degradation of formaldehyde or high density degradation of formaldehyde, so major part all is to close degradation of formaldehyde bacterium and the saccharomycetic research of degradation of formaldehyde, and about degradation of formaldehyde The research of molds report seldom.
Kondo etc. (2002) have separated strain formaldehyde tolerance fungi Aspergillus nomiusIRI013 from soil, it can be grown in the highest w
(formaldehyde)=0.45% li and its completely consumed fallen.The research of domestic this respect was also gradually many over the past two years, people such as Huang Saihua have a series of technology reports, be separated to Degradation Formaldehyde fungi (2007) from Furniture Factory's water outlet that is untreated, work out 3 strain formaldehyde and transform mould Trichoderma viride H1, Penicillium javanicum H2, Aspergillus flavus H4, all can be grown in formaldehyde is on the substratum of sole carbon source, can be with concentration of formaldehyde respectively by 1047mg/L, 1059mg/L and 1241mg/L are converted into 7.6mg/L, 317mg/L and 4.0mg/L, transformation efficiency is respectively 99.3%, 70.1% and 99.7% (2008), can both utilize 4 kinds of compounded carbonses (0.1% formaldehyde+10% sucrose, 0.1% formaldehyde+10% maltose, 0.1% formaldehyde+10% starch, 0.1% formaldehyde+10% glucose) and transform formaldehyde, its transformation efficiency is respectively 99.6%, more than 74.9% and 99.3% (2009).
But biological treatment engineering demand with rapid changepl. never-ending changes and improvements along with the formaldehyde pollution, it is very not enough that above-mentioned degradation of formaldehyde bacterium database seems, presses for more technical study achievements about the degradation of formaldehyde bacterium and provide strong help for the biological treatment engineering that formaldehyde pollutes.
Summary of the invention
An object of the present invention is the deficiency at existing Degradation Formaldehyde bacterium technology, a kind of new Degradation Formaldehyde bacterium is provided, described Degradation Formaldehyde bacterium is easily cultivated, formaldehyde transformation efficiency height.
Another object of the present invention provides the application of described Degradation Formaldehyde bacterium.
Technical scheme of the present invention is achieved by following technology:
A kind of new Degradation Formaldehyde bacterium is provided, be paecilomyces varioti (Paecilomyces variotii) bacterial strain, in No. 3 China Committee for Culture Collection of Microorganisms's common micro-organisms center preservations on April 9th, 2010 in the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preserving number is CGMCC No.3722, called after paecilomyces varioti (Paecilomyces variotii) H6.
Flat board observation feature and the microscopic examination feature of described paecilomyces varioti (Paecilomyces variotii) H6 are as follows:
H6 is fast growth on czapek's solution, cultivates 7 days colony diameter diameter 6cm, and surface soil yellow, quality are that pine is cotton-shaped to rope form, and reverse side is filbert, and the old back of mycelia produces the transudate of chocolate in the surface; Colony growth is fast on wort agar, cultivates 7 days colony diameter diameter 8cm, surperficial oyster (comparing the band green hue with bacterium colony on the czapek's solution), and the quality pine is cotton-shaped to strand; On Cha Shi yeast extract paste agar, cultivate 7 days colony diameter diameter 8cm, the surface soil redness, the quality pine is cotton-shaped, the reverse side tawny.It is big that microscopically can be observed the conidium textural difference, and one stigma and complicated repeatedly broom shape branch are arranged; Conidiophore bears from aerial hyphae or rope form funiculus, and the elongated gradually point of stigma is normal crooked,
The conidium ellipse,
Smooth.
The 18Sr DNA aligned sequences of described paecilomyces varioti (Paecilomyces variotii) H6 is seen shown in the SEQ ID NO:1.Its 18SrDNA sequence and paecilomyces varioti (Paecilomyces variotii) homology reaches 100%.
The present invention provides the Degradation Formaldehyde characteristic test of described paecilomyces varioti (Paecilomyces variotii) H6 simultaneously, formaldehyde transformation efficiency by having experimental results demonstrate H6 is very high, can be used as good Degradation Formaldehyde bacterium and is applied to the biological treatment aspect that formaldehyde pollutes.
The invention has the beneficial effects as follows:
The invention provides a kind of new paecilomyces varioti (Paecilomyces variotii) H6, described bacterial strain has the ability of good degradation of formaldehyde, has greatly replenished the database of degradation of formaldehyde bacterium; Described paecilomyces varioti (Paecilomyces variotii) H6 has a very wide distribution at occurring in nature, and breeding is rapid, and energy for growth is strong.
The invention provides the application of paecilomyces varioti (Paecilomyces variotii) H6 aspect Degradation Formaldehyde, the biological treatment engineering for formaldehyde pollutes provides powerful technical support.
Description of drawings
Fig. 1 H6 is the mycelial growth situation on czapek's solution
Fig. 2 H6 is the mycelial growth situation on wort agar
Fig. 3 H6 is the mycelial growth situation on Cha Shi yeast extract paste agar
Fig. 4 H6 microscopically spore structure
H6 degradation curve in the different concentration of formaldehyde substratum of Fig. 5
The blank curve of the different concentration of formaldehyde substratum of Fig. 6
H6 growth curve in the different concentration of formaldehyde substratum of Fig. 7
Embodiment
Further describe the present invention below in conjunction with the drawings and specific embodiments.
Separation and the evaluation of embodiment 1 Degradation Formaldehyde bacterium one paecilomyces varioti of the present invention (Paecilomyces variotii) H6
1, culture medium prescription
Minimum medium: 1.0% (wv
-1) glucose, 0.2% (wv
-1) NaNO
3, 0.1% (wv
-1) K
2HPO
4, 0.05% (wv
-1) MgSO
47H
2O, 0.05% (wv
-1) KCl, 0.001% (wv
-1) FeSO
47H
2O, 0.01% (wv
-1) yeast extract paste, 0.1% (wv
-1) formaldehyde (formaldehyde with commercially available formaldehyde standardized solution), the substratum final pH is 6.0.(as not specifying, following minimum medium all fill a prescription substratum) for this reason
Czapek's solution prescription: 3.0% (wv
-1) sucrose, 0.3% (wv
-1) NaNO
3, 0.1% (wv
-1) K
2HPO
4, 0.05% (wv
-1) MgSO
47H
2O, 0.05% (wv
-1) KCl, 0.001% (wv
-1) FeSO
47H
2O, 1.5～2.0% (wv
-1) agar.
Malt extract medium prescription: 2.0% (wv
-1) malt extract, 0.1% (wv
-1) peptone, 2.0% (wv
-1) glucose, 1.5～2.0% (wv
-1) agar.
Cha Shi yeast extract paste agar prescription: K
2HPO
40.1% (wv
-1), yeast extract paste 0.05% (wv
-1), sucrose 3.0% (wv
-1), the dense liquid storage 1%ml of Cha Shi (wv
-1), 1.5～2.0% (wv
-1) agar.The pH nature.The dense liquid storage prescription of Cha Shi: NaNO wherein
330g, KCl 5g, FeSO
47H
2O0.1g, MgSO
47H
2O 5g, water 100mL.
2, separation and purification
Get vegetable garden soil 1kg at random, dry in the air to 7～8 one-tenth and do 0.1% (wv
-1) formaldehyde solution 200mL, evenly add in the soil sample of vegetable garden with dropper, repeat to add one time 0.1% formaldehyde solution 200mL after 4～5 days again, about mixing sampling 100g, on Bechtop, get 10g and place the 90mL sterilized water after 3～4 days through the soil sample of 0.1% formaldehyde domestication, vibration 15min, placed 20 seconds, and got the 1mL supernatant liquor and be inoculated in the minimum medium, 160rmin on shaking table
-1Cultivate 5d for 30 ℃, the fungi that growth is got up is repeatedly used the setting-out of PDA culture medium flat plate again, through purifying repeatedly, is separated to a strain mould, numbering H6.Then bacterial strain H6 is inoculated in respectively on czapek's solution, malt extract medium, the Cha Shi yeast extract paste agar, cultivates 8d, observe its colony characteristics for 25 ℃.Simultaneously, microscopically is observed microstructure, and has write down constitutional features.
H6 is fast growth on czapek's solution, cultivates 7 days colony diameter diameter 6cm, and surface soil yellow, quality are that pine is cotton-shaped to rope form, and reverse side is filbert, and the old back of mycelia produces the transudate of chocolate in the surface, see shown in the accompanying drawing 1;
H6 colony growth on wort agar is fast, cultivates 7 days colony diameter diameter 8cm, and surperficial oyster is compared the band green hue with bacterium colony on the czapek's solution, and the quality pine is cotton-shaped to strand, sees shown in the accompanying drawing 2;
H6 cultivates 7 days colony diameter diameter 8cm on Cha Shi yeast extract paste agar, the surface soil redness, and the quality pine is cotton-shaped, and the reverse side tawny is seen shown in the accompanying drawing 3.
Microscopically is observed, and H6 conidium textural difference is big, and one stigma and complicated repeatedly broom shape branch are arranged; Conidiophore bears from aerial hyphae or rope form funiculus, and the elongated gradually point of stigma is normal crooked,
The conidium ellipse,
Smooth, see shown in the accompanying drawing 4.
Extract DNA, detect DNA concentration and purity, a series of Molecular Identification such as PCR 18S rDNA amplification, the 18Sr DNA aligned sequences of described paecilomyces varioti (Paecilomyces variotii) H6 is seen shown in the SEQ ID NO:1,18SrDNA sequence of the degradation of formaldehyde bacterium that separation and purification of the present invention obtains and paecilomyces varioti (Paecilomyces variotii) homology reaches 100%, and its cultural characteristic and microscopic features are also the most similar to paecilomyces varioti (Paecilomyces variotii).
This shows that the degradation of formaldehyde bacterium that the present invention screens is paecilomyces varioti (Paecilomycesvariotii).
The degradation experiment of embodiment 2 paecilomyces variotis of the present invention (Paecilomyces variotii) H6 PARA FORMALDEHYDE PRILLS(91,95)
1, first order seed produces
Choose and produce good paecilomyces varioti (Paecilomyces variotii) H6 of spore, be seeded in the minimum medium shaking table 160rmin
-1, cultivate 2～3d for 30 ℃, when mould enters fast growing period, regularly measure OD
475Value is worked as OD
475During the value fast rise, this bacterium liquid promptly can be used as first order seed.
2, experimental program
Choose big mouthful of 150mL triangular flask, preparation minimum medium, C
(formaldehyde)By about 0.1%, 0.15%, 0.2%, 0.25% (wv
-1) adding (C
FormaldehydeBe as the criterion with actual measurement data), bottleneck seals with the aseptic culture membrane that seals, and cultivates first order seed (OD
475Value is 0.915), every bottle graft is gone into 5mL except that blank, puts 30 ℃ of 160rmin on the shaking table
-1Cultivate, and inoculation the 0th, 24,48,72,96,120,144h takes a sample respectively, gets 3 bottles at every turn, wherein one bottle be blank, the centrifugal 15min of 7000 * very low temperature, the filtration earlier of sampling back, filtrate is with AHMT spectrophotometry detection C
Formaldehyde, filter paper and bacterium change over to together and have claimed to survey the mycelia dry weight to the beaker of constant weight, and the mycelia dry weight is measured with weighting method, and the result gets its mean value mapping.
The OD pH-value determination pH TU-1800 of Beijing Puxi General Instrument Co., Ltd type ultraviolet-visible pectrophotometer, the mycelia dry weight is measured with weighting method, and the result gets its mean value mapping.
The results are shown in shown in accompanying drawing 5～accompanying drawing 7.Wherein, accompanying drawing 5 is the H6 degradation curve in the different concentration of formaldehyde substratum, and accompanying drawing 6 is the blank curve of different concentration of formaldehyde substratum, the H6 growth curve in the accompanying drawing 7 different concentration of formaldehyde substratum.
By accompanying drawing 5～accompanying drawing 7 as can be known:
The volatilization of the blank of 4 concentration in 144h seldom can be ignored substantially, sees shown in the accompanying drawing 6.
When initial concentration of formaldehyde was 1245mg/L, 144h dropped to 87.5mg/L, and degradation rate is 93.0%, and degraded is seen shown in the accompanying drawing 5 behind 48h fast; The mycelia dry weight also rises to 0.5422g from 0.4902g, has increased 0.0520g, increases behind 72h fast, sees shown in the accompanying drawing 7; When initial concentration of formaldehyde was 1658mg/L, 144h dropped to 451mg/L, and degradation rate is 72.8%, and degraded is behind 48h fast, and the mycelia dry weight also rises to 0.5223g from 0.4826g, has increased 0.0397g, increased behind 96h fast; When initial concentration of formaldehyde is respectively 2376, during 2880mg/L, 144h drops to 1623,2021mg/L, degradation rate is respectively 31.7,29.8%, does not have quick degradative phase, the mycelia dry weight increases slowly, does not significantly increase fast.
Claims (3)
1. a paecilomyces varioti (Paecilomyces variotii) bacterial strain H6 is preserved in China Committee for Culture Collection of Microorganisms on April 9th, 2010, and preserving number is CGMCCNo.3722.
2. the sequence table of the described paecilomyces varioti of claim 1 (Paecilomyces variotii) bacterial strain H6.
3. the application of the described paecilomyces varioti of claim 1 (Paecilomyces variotii) bacterial strain H6 is characterized in that being applied to degradation of formaldehyde.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533563A (en) * | 2011-11-08 | 2012-07-04 | 中国科学院微生物研究所 | Celluase producing bacterium and application thereof |
| CN103710273A (en) * | 2014-01-13 | 2014-04-09 | 常熟理工学院 | Paecilomyces variot bainier F1-23 and method for processing methanal-containing industrial wastewater by using same |
| CN105879658A (en) * | 2016-05-12 | 2016-08-24 | 广州荣天环保科技有限公司 | Preparation method of acetone degradation agent containing complex microorganisms and biological enzymes |
| CN111394260A (en) * | 2020-04-29 | 2020-07-10 | 常州市新鸿医药化工技术有限公司 | Separation and application of microorganisms for treating wastewater |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250488A (en) * | 2008-04-14 | 2008-08-27 | 广东省生态环境与土壤研究所 | Use of Paecilomyces javanicus H2 in degradation of formaldehyde |
-
2010
- 2010-08-13 CN CN 201010253652 patent/CN102021120B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250488A (en) * | 2008-04-14 | 2008-08-27 | 广东省生态环境与土壤研究所 | Use of Paecilomyces javanicus H2 in degradation of formaldehyde |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533563A (en) * | 2011-11-08 | 2012-07-04 | 中国科学院微生物研究所 | Celluase producing bacterium and application thereof |
| CN102533563B (en) * | 2011-11-08 | 2013-04-10 | 中国科学院微生物研究所 | Celluase producing bacterium and application thereof |
| CN103710273A (en) * | 2014-01-13 | 2014-04-09 | 常熟理工学院 | Paecilomyces variot bainier F1-23 and method for processing methanal-containing industrial wastewater by using same |
| CN103710273B (en) * | 2014-01-13 | 2015-05-27 | 常熟理工学院 | Paecilomyces variot bainier F1-23 and method for processing methanal-containing industrial wastewater by using same |
| CN105879658A (en) * | 2016-05-12 | 2016-08-24 | 广州荣天环保科技有限公司 | Preparation method of acetone degradation agent containing complex microorganisms and biological enzymes |
| CN105879658B (en) * | 2016-05-12 | 2018-06-15 | 广州荣天环保科技有限公司 | A kind of preparation method of the Acetone decomposition agent of complex microorganism and biological enzyme |
| CN111394260A (en) * | 2020-04-29 | 2020-07-10 | 常州市新鸿医药化工技术有限公司 | Separation and application of microorganisms for treating wastewater |
| CN111394260B (en) * | 2020-04-29 | 2022-08-09 | 常州市新鸿医药化工技术有限公司 | Separation and application of microorganisms for treating wastewater |
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