CN103710273A - Paecilomyces variot bainier F1-23 and method for processing methanal-containing industrial wastewater by using same - Google Patents
Paecilomyces variot bainier F1-23 and method for processing methanal-containing industrial wastewater by using same Download PDFInfo
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- CN103710273A CN103710273A CN201410012922.4A CN201410012922A CN103710273A CN 103710273 A CN103710273 A CN 103710273A CN 201410012922 A CN201410012922 A CN 201410012922A CN 103710273 A CN103710273 A CN 103710273A
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Abstract
The invention belongs to field of microorganisms and application of the microorganisms, and particularly relates to paecilomyces variot bainier F1-23 and a method for processing methanal-containing industrial wastewater by using the paecilomyces variot bainier F1-23. The paecilomyces variot bainier F1-23 CCTCC No.M2013471 or a carrier fixed with the paecilomyces variot bainier F1-23 CCTCC No.M2013471 is inoculated into the methanal-containing industrial wastewater for degrading the methanal in the methanal-containing industrial wastewater after mixing and vibrating or fully contacting; after degradation, the content of methanal in the industrial wastewater is less than 6.0g/L. The method solves the problem that the existing microorganism only degrades the industrial wastewater with low concentration of methanal, and the paecilomyces variot bainier F1-23 has the advantages of having high capacity in degrading methanal, having good heredity, and degrading high concentration methanal-containing industrial wastewater through thallus fixation and circular process.
Description
Technical field
The invention belongs to microorganism and application, refer to especially a kind of paecilomyces varioti bacterium F1-23 and utilize it to process the method containing formaldehyde industrial wastewater.
Background technology
Formaldehyde (HCHO), under normal temperature, for having pungency and asphyxiant colourless gas, commodity are its aqueous solution, one of ten kinds of chemical that output is the highest in the world.Its aqueous solution is generally used as sterilization, anticorrosion and fumigant in agricultural, forestry, livestock industry, biology and medicine.Formaldehyde is harmful, be mainly because formaldehyde can with human body in protein bound, change protein interior structure and also it solidified, thereby there is lethality.At present, the method except formaldehyde mainly contains chemical reaction method, physical adsorption techniques, ozone anion technology, nano photo catalyzed oxidation, plasma technology etc.; These methods all exist degraded not thoroughly, long, high in cost of production shortcoming (the Saeed M. of degradation time, 2005.World Journal of Microbiology and Biotechnology.21:1299-1301.) probiotics that microorganism rule be take in ecotope is material degradation formaldehyde, cost is low, effective, and non-secondary pollution.
The beginning of this century, adopt microbiological deterioration formaldehyde just to be paid close attention to day by day widely.In microorganism, extensively exist an oxidation of formaldehyde approach (Barber RD., 1998.Journal of Molecular Biology.280(5): 775-784.), act on key enzyme formaldehyde dehydrogenase (the formaldehyde dehydrogenase of this approach, FADH, EC1.2.1.1) be in medium Xin Lian alcoholdehydrogenase family, be present in most protokaryons and all eukaryotic microorganisms (Barber RD., 1996.Journal of Bacteriology.178(5): 1386-1393.).Formaldehyde removing toxic substances for microorganism has vital role, is also the key link of current microorganisms degradation of formaldehyde.Currently reported Degradation Formaldehyde bacterium major part is Rhodopseudomonas (Pseudomonas sp.) (Saeed M., 2005.World Journal of Microbiology and Biotechnology.21:1299-1301; Adroer N., 1990.Applied Microbiology and Biotechnology.33:217-220; Xie Wenjuan, waits .2011. microbiology circular .38 (11): 1626-1631; The .2010. environmental science .31(10 such as Xu Yun): 2481-2486; The .2011. environmental engineering journal .5(11 such as Li Zhangliang): 2547-2551.), the concentration that these Institute of Micro-biology can degrade is lower, be generally 0.1-0.8g/L, and the concentration of formaldehyde content of trade effluent is generally 3g/L, this just needs the bacterial strain that seed selection tolerance concentration is higher, degradation capability is stronger.
Traditional microorganism method for artificially breeding has selection by mutation, cross-breeding (comprising protoplast fusion) and genetic engineering breeding.Wherein selection by mutation is the most extensive, to adopt physics or chemical factor to process microorganism cells colony, impel hereditary thing (DNA) structure in a few cell wherein to change, thereby cause that microorganism hereditary proterties changes, and then filters out the process of good mutant strain from colony.By existing breeding method, obtained the mutant strain of Partial digestion high-concentration formaldehyde, and these methods will become the Critical policies of strain improvement.
At present; report that more is that formaldehyde waste water can pass through aerobic (Hidalgo A.; 2002.Applied Microbiology and Biotechnology.58(2): 260-263.) or anaerobism mode (Mingbo QU., 1997.Biotechnology and Bioengineering.55(5-6): 727-736; Omil F., 1991.Enzyme Microbiol.24(5-6): 255-262.) carry out biological degradation, mainly adopt active sludge PARA FORMALDEHYDE PRILLS(91,95) waste water to process (Wang Zhihai etc., 2009. Chinese water supply and drainage .25(1): 55-57; The impartial .2008. environmental engineering of Xu Zhong journal .2(9): 1174-1176; Eiroa M., 2005.Bioresource Technology.96(17): 1914-1918.), and almost do not have about the report of using microbe immobilization technology degradation of formaldehyde waste water.With microbial immobilized technical finesse sewage, compare with other improvement technology, there is the removal efficiency that improves pollutent, alleviate the burden of follow-up sludge disposal, be conducive to the mud-water separation of precipitation process, the features such as, good stability strong to the ability to bear of toxic substance.Therefore, microbial immobilized technology has become the study hotspot (in king difficult to understand etc., the 2004. journal .27(3 of University Of Chongqing) of domestic and international field of waste water treatment: 125-129; Zhang Lei etc., the 2009. journal .26(2 of Hubei University of Education): 39-42.).
Summary of the invention
One of object of the present invention is to provide a kind of paecilomyces varioti bacterium F1-23.
Two of object of the present invention is to provide utilizes paecilomyces varioti bacterium F1-23 to process the method containing formaldehyde industrial wastewater.
Overall technology design of the present invention is:
A kind of paecilomyces varioti bacterium F1-23(paecilomyces variotii F1-23), its deposit number is CCTCC No.M2013471.
Utilize paecilomyces varioti bacterium to process the method containing formaldehyde industrial wastewater, paecilomyces varioti bacterium F1-23 CCTCC No.M2013471 is maybe contained the carrier access that is fixed with paecilomyces varioti bacterium F1-23CCTCC No.M2013471 in formaldehyde industrial wastewater, mix oscillation treatment or fully after contact, degrade containing the formaldehyde in formaldehyde industrial wastewater; In described trade effluent, the content of formaldehyde is lower than 6.0g/L.
Concentration of formaldehyde is too high, also can suppress the degradation property of paecilomyces varioti bacterium F1-23CCTCC No.M2013471, so the concentration of formaldehyde solution can not be higher than 6.0g/L.
Mentioned microorganism is submitted Chinese Typical Representative culture collection center (being called for short CCTCC) preservation on October 13rd, 2013, and its deposit number is CCTCC No.M2013471, and the address of this depositary institution is positioned at Wuhan City, Hubei Province Wuhan University.
The morphological specificity of mentioned microorganism is: chemoheterotrophy; Have a liking for temperature; Acid becomes.Cultivate 5d for 25 ℃, bacterium colony is flat thin, coarse, is slightly particulate state; Outer rim is white, and inside is brownish black; Dry without transudate, there is irregular radiation fold rill on surface.In mycelia, there is no diaphragm; The μ m of sporangiospore (4-5) * (2-3), ellipse, is cystic structures.
Concrete technical conceive of the present invention also has:
The solid medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Agar 18-20; Water 1000; PH=7.2-7.5;
The liquid nutrient medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass umber to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Water 1000; PH=7.2-7.5.
Described carries out, before degradation of formaldehyde processing, also comprising a pre-treatment step containing formaldehyde industrial wastewater, and this pre-treatment step is for reducing the concentration of the COD in trade effluent, BOD and metal ion.
The described pre-treatment containing formaldehyde industrial wastewater can adopt multiple existing techniques in realizing, consider the cost of application and time, and the waste water after active sludge treatment is little on the impact of paecilomyces varioti bacterium F1-23CCTCC No.M2013471, pre-treatment step preferably adopts following technique to realize:
A, collection active sludge
From sewage work, gather active sludge;
B, mixing shaking culture
Active sludge is mixed with the trade effluent containing formaldehyde, carry out shaking culture;
C, standing sedimentation
By the product standing sedimentation after shaking culture in step B, by recycling precipitate recycling, supernatant liquor is for the degradation treatment of formaldehyde.
Described carrier refers to the porous material thalline of paecilomyces varioti bacterium CCTCC No.M2013471 to height adsorptive power.Described carrier preferably adopts but is not limited to tubular fibre, kaolin, sintered glass, gac, silica gel.
Described paecilomyces varioti bacterium CCTCC No.M2013471 fixedly comprising the steps: on carrier
A, carrier is placed in to the liquid nutrient medium that contains paecilomyces varioti bacterium CCTCC No.M2013471 cultivates;
B, paecilomyces varioti bacterium CCTCC No.M2013471 are adsorbed in the immobilization that completes thalline on carrier.
The measuring method of degradation of formaldehyde ability is as follows, utilizes this method as the evaluation basis of screening paecilomyces varioti bacterium F1-23CCTCC No.M2013471.
The mensuration of formaldehyde, formaldehyde reacts with methyl ethyl diketone and ammonium ion and generates yellow substance under pH=5.5-7.0 condition, and at wavelength, 414nm has absorption peak, more quantitative with standard series.
(1) demarcation of sulfuric acid standard titration solution (using anhydrous sodium carbonate standardization): while configuring the sulfuric acid standard titration solution of 0.5mol/L, get 98% vitriol oil 72ml, along filling in the slow injected water of walls of beaker of distilled water; Treat that solution temperature is down to room temperature, then adding distil water is settled to 1L, shakes up.While demarcating concentration, take the benchmark anhydrous sodium carbonate 1.1g drying to constant weight through 60 ℃, put in 250ml iodine flask, adding distil water 50ml makes to dissolve.Methylate is red-and tetrabromo-mcresolsulfonphthalein mixes indicating liquid 1-2 and drips, with the sulfuric acid titrating solution titration of preparation.Until solution, when green becomes red-purple, boil 2min.Be cooled to room temperature, continue to be titrated to solution when green becomes mulberry, record the consumption of sulfuric acid titrating solution.The mean value of getting three replicate(determination) results is net result, and the error between Duplicate Samples must not be greater than 0.1%.
The sulfuric acid titrating solution concentration c representing with molar mass, is calculated as follows:
c（mol/L）=m/（0.1060×γ）
In formula: m---anhydrous sodium carbonate quality, g;
γ---sulfuric acid titrating solution volume, ml;
0.1060---1mol/L sulfuric acid titrating solution 1ml is equivalent to 0.1060g anhydrous sodium carbonate.
(2) demarcation of formaldehyde standardized solution: formaldehyde and excessive neutral sodium sulfite solution reaction, generate sodium hydroxide, using thymolphthalein as indicator, with the titration of sulfuric acid titrand.During mensuration, drip thymolphthalein indicating liquid with adding 50ml sodium sulfite solution and 2-3 in 250ml Erlenmeyer flask, with sulfuric acid standard titration solution, be neutralized to blueness and just disappear.By decrement method, take 1.3-1.5g formaldehyde standardized solution, be accurate to 0.0002g, put into above-mentioned Erlenmeyer flask, with sulfuric acid standard titration solution, being neutralized to that blueness just disappeared is terminal.The arithmetical av of getting three replicate(determination) results is measurement result.The error of three replicate(determination) results must not be higher than 0.1%.
With mass percent, represent that formaldehyde content x is calculated as follows:
x=γ×c×2×100×0.3003/m=γ×c×6.006/m
In formula: γ---titration consumes the volume of sulfuric acid standard titration solution, ml;
C---the reagent concentration of sulfuric acid standard titration solution, mol/L;
The quality of m---sample, g;
0.03003---with 1.00ml sulfuric acid standard titration solution ［ c(1/2H
2sO
4)=1.000mol/L ］ suitable formaldehyde quality (g).
(3) mensuration of formaldehyde typical curve: get 6 of clean tube, get 0,0.05,0.10,0.15,0.20 and the above-mentioned formaldehyde reference liquid of 0.25ml in test tube, add distilled water to 5ml, add methyl ethyl diketone 1ml, in 55 ℃ of water-baths, boil 15min, the reaction times needs strict control.With blank tube suppressed zero, in 414nm place, measure light absorption value A, take concentration as X-coordinate, A value is ordinate zou, draws out typical curve.
While detecting bacterial classification, thalline access in inclined-plane is equipped with in the 250ml Erlenmeyer flask of 50ml experiment substratum, 28 ℃, 180rpm are cultivated 5d.Filtering fermentation liquor, filtrate is according to standard curve determination residual formaldehyde content.
The substantive distinguishing features that the present invention is obtained and significant technical progress are:
1, the paecilomyces varioti bacterium F1-23CCTCC No.M2013471 in the present invention is improved on HCHO-resistance and degradation capability, is divided into and has improved 22.2% and 23.8%, and genetic stability is good.
2, the present invention adopts the fixing fixing paecilomyces varioti bacterium F1-23CCTCC No.M2013471 of tubular fibre of carrier possess stronger adsorptive power, (2h) degradation of formaldehyde (3.725g/L) at short notice, this concentration is higher than the formaldehyde content (3%) of common industrial waste water.For the trade effluent of high-concentration formaldehyde content, can solve by the mode of circular treatment.
3, in addition, the present invention uses microbiological deterioration as degradation of formaldehyde major way, in operational process, can not produce secondary pollution, can reuse, and saves production cost, and is conducive to mass-producing and expands.
Described in the present invention in submission on October 13rd, 2013 Chinese Typical Representative culture collection center (being called for short CCTCC) preservation, its deposit number is CCTCC No.M2013471.
Embodiment
Below in conjunction with embodiment, the present invention is described further; but should not be construed as limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion, and any equivalence techniques means of having done according to specification sheets are replaced, and all do not depart from protection scope of the present invention.
Embodiment 1
A kind of paecilomyces varioti bacterium F1-23(paecilomyces variotii F1-23), its deposit number is CCTCC No.M2013471.
Utilize paecilomyces varioti bacterium to process the method containing formaldehyde industrial wastewater, paecilomyces varioti bacterium F1-23CCTCC No.M2013471 is maybe contained the carrier access that is fixed with paecilomyces varioti bacterium F1-23CCTCC No.M2013471 in formaldehyde industrial wastewater, mix oscillation treatment or fully after contact, degrade containing the formaldehyde in formaldehyde industrial wastewater; In described trade effluent, the content of formaldehyde is lower than 6.0g/L.
The solid medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Agar 18-20; Water 1000; PH=7.2-7.5;
The liquid nutrient medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass umber to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Water 1000; PH=7.2-7.5.
Described carries out, before degradation of formaldehyde processing, also comprising a pre-treatment step containing formaldehyde industrial wastewater, and this pre-treatment step is for reducing the concentration of the COD in trade effluent, BOD and metal ion.
The described pre-treatment containing formaldehyde industrial wastewater can adopt multiple existing techniques in realizing, consider the cost of application and time, and the waste water after active sludge treatment is little on the impact of paecilomyces varioti bacterium F1-23CCTCC No.M2013471, pre-treatment step preferably adopts following technique to realize:
A, collection active sludge
From sewage work, gather active sludge;
B, mixing shaking culture
The ratio that is 1:2 with the trade effluent that contains formaldehyde according to mass ratio by active sludge is mixed, at 25 ℃, 150rpm shaking culture 4-6 hour;
C, standing sedimentation
By the product standing sedimentation after shaking culture in step B, by recycling precipitate recycling, supernatant liquor is for the degradation treatment of formaldehyde.
Adopt prepared by the aforesaid method liquid nutrient medium that contains paecilomyces varioti bacterium F1-23CCTCC No.M2013471 to carry out result to the different trade effluent of following formaldehyde content as follows:
1, concentration of formaldehyde 1.12g/L, thalline inoculum size 10%, mixes vibration 12h, and formaldehyde is thoroughly degraded.
2, concentration of formaldehyde 2.24g/L, thalline inoculum size 10%, mixes vibration 36h, and formaldehyde is thoroughly degraded.
3, concentration of formaldehyde 3.73g/L, thalline inoculum size 10%, mixes vibration 36h, and formaldehyde is thoroughly degraded.
4, concentration of formaldehyde 5.60g/L, thalline inoculum size 10%, mixes vibration 36h, Degradation Formaldehyde rate 69%; Thalline inoculum size 20%, mixes vibration 36h, thoroughly degradation of formaldehyde.
Embodiment 2
In the present embodiment the cultivation of bacterial classification, bacterial classification and containing the pre-treatment of formaldehyde industrial wastewater as embodiment 1, the tubular fibre of 10 long 10cm (aperture is 0.1 μ m) is restrainted into a bundle, insert in same liquid nutrient medium, under 25 ℃, 180 revs/min conditions, cultivate 3-5 days, cause paecilomyces varioti bacterium F1-23CCTCC No.M2013471 to be adsorbed on tubular fibre, complete immobilization process.In gnotobasis, chopping is attached with paecilomyces varioti bacterium F1-23CCTCC No.M2013471(biomass and is about 0.1g) tubular fibre, be filled in the pillar (ψ=13mm, L=10cm) of hollow; By peristaltic pump, take the formaldehyde solution that 0.45ml/min flow velocity is 3.725g/L by concentration and inject pillar, when liquid flows out pillar, hardly containing formaldehyde.
Adopt said apparatus to process containing after the trade effluent 2h of formaldehyde, use washed with de-ionized water pillar, can again recycle afterwards.
Meanwhile, tested the formaldehyde solution lower than 3.725g/L containing concentration of formaldehyde, this device of being flowed through, equal thorough formaldehyde in degraded solutions in 2h, and can Reusability several.
For the higher paper mill effluent of dustiness, its formaldehyde content, up to 5g/L, is processed this waste water with said apparatus, and in 2h, Degradation Formaldehyde is 70%, and the Formaldehyde In Wastewarter flowing out is 1.5g/L; Again wastewater streams is carried out to secondary treatment through device, its formaldehyde of can thoroughly degrading.This reusable advantage is that other removal formaldehyde methods are not available.
There is substantial connection the time (being the immobilization time) of thalline absorption with kind of carrier, and design parameter is as shown in the table.
For proof paecilomyces varioti bacterium F1-23CCTCC No.M2013471 is fixed on carrier and is directly inoculated in containing whether there being difference in the trade effluent of formaldehyde, applicant has carried out following experiment.
From solid medium, picking paecilomyces varioti bacterium F1-23CCTCC No.M2013471, to its liquid nutrient medium, cultivates 3-5 days under 25 ℃, 180 revs/min conditions, obtains mycelium pellet; Get in the formaldehyde solution that 5ml liquid medium (containing the mycelium pellet of some amount) to concentration of formaldehyde is 3.725g/L; And the tubular fibre of 10 long 10cm (aperture is 0.1 μ m) is restrainted into a bundle, insert in same liquid nutrient medium, under 25 ℃, 180 revs/min conditions, cultivate 3-5 days, cause paecilomyces varioti bacterium F1-23CCTCCNo.M2013471 to be adsorbed on tubular fibre, complete immobilization process.
Immobilized paecilomyces varioti bacterium F1-23CCTCC No.M2013471 can be in 2 hours formaldehyde solution that thoroughly degradation of formaldehyde concentration is 3.725g/L, show immobilization way on the original Degradation Formaldehyde performance of microorganism without impact.
Claims (8)
1. a paecilomyces varioti bacterium F1-23, is characterized in that its deposit number is CCTCC No.M2013471.
2. paecilomyces varioti bacterium F1-23 according to claim 1, is characterized in that the solid medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Agar 18-20; Water 1000; PH=7.2-7.5;
The liquid nutrient medium of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 adopts the component of following unit mass umber to form:
Glucose 10-30; Extractum carnis 2-4; KCl0.5; MgSO
47H
2o1; FeSO
47H
2o0.1; Water 1000; PH=7.2-7.5.
3. utilize paecilomyces varioti bacterium F1-23 to process the method containing formaldehyde industrial wastewater, it is characterized in that paecilomyces varioti bacterium F1-23CCTCC No.M2013471 maybe the carrier access that is fixed with paecilomyces varioti bacterium F1-23CCTCC No.M2013471 to be contained in formaldehyde industrial wastewater, mix oscillation treatment or fully after contact, degrade containing the formaldehyde in formaldehyde industrial wastewater; In described trade effluent, the content of formaldehyde is lower than 6.0g/L.
4. the method for utilizing paecilomyces varioti bacterium F1-23 to process containing formaldehyde industrial wastewater according to claim 3 is characterized in that described carrying out before degradation of formaldehyde processing containing formaldehyde industrial wastewater, also comprise a pre-treatment step, this pre-treatment step is for reducing the concentration of the COD in trade effluent, BOD and metal ion.
5. the method for utilizing paecilomyces varioti bacterium F1-23 to process containing formaldehyde industrial wastewater according to claim 4 is characterized in that described pre-treatment step comprises the steps:
A, collection active sludge
From sewage work, gather active sludge;
B, mixing shaking culture
Active sludge is mixed with the trade effluent containing formaldehyde, carry out shaking culture;
C, standing sedimentation
By the product standing sedimentation after shaking culture in step B, by recycling precipitate recycling, supernatant liquor is for the degradation treatment of formaldehyde.
6. the method for utilizing paecilomyces varioti bacterium F1-23 to process containing formaldehyde industrial wastewater according to claim 3 is characterized in that described carrier refers to the porous material thalline of paecilomyces varioti bacterium F1-23CCTCC No.M2013471 to adsorptive power.
7. according to utilizing paecilomyces varioti bacterium F1-23 to process method containing formaldehyde industrial wastewater to it is characterized in that described carrier is selected from a kind of in tubular fibre, kaolin, sintered glass, gac, silica gel described in claim 3 or 6.
8. the method for utilizing paecilomyces varioti bacterium F1-23 to process containing formaldehyde industrial wastewater according to claim 3 is characterized in that described paecilomyces varioti bacterium F1-23CCTCC No.M2013471 fixedly comprising the steps: on carrier
A, carrier is placed in to the liquid nutrient medium that contains paecilomyces varioti bacterium F1-23CCTCC No.M2013471 cultivates;
B, paecilomyces varioti bacterium F1-23CCTCC No.M2013471 are adsorbed in the immobilization that completes thalline on carrier.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531666A (en) * | 2014-08-29 | 2015-04-22 | 华南理工大学 | Preparation method and application of kaolin immobilized GY2B bacterium |
| CN111218405A (en) * | 2018-11-27 | 2020-06-02 | 成功大学 | Method for producing paecilomyces rhododendron mycelium, bioplastic, and wastewater treatment method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021120A (en) * | 2010-08-13 | 2011-04-20 | 广东省生态环境与土壤研究所 | Paecilonyces variotii strain and application thereof |
| RU2448107C2 (en) * | 2010-06-02 | 2012-04-20 | Учреждение Российской Академии Наук Институт Нефтехимии И Катализа Ран | Method of producing 3,3'-bi-1,5,3-dithiazepinane and use thereof as fungicidally active agent |
-
2014
- 2014-01-13 CN CN201410012922.4A patent/CN103710273B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2448107C2 (en) * | 2010-06-02 | 2012-04-20 | Учреждение Российской Академии Наук Институт Нефтехимии И Катализа Ран | Method of producing 3,3'-bi-1,5,3-dithiazepinane and use thereof as fungicidally active agent |
| CN102021120A (en) * | 2010-08-13 | 2011-04-20 | 广东省生态环境与土壤研究所 | Paecilonyces variotii strain and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| J.PRADO ET AL: ""REMOBAL OF FORMALDEHYDE,METHANOL,DIMETHYLETHER AND CARBON MONOXIDE FROM WASTE GASES OF SYNTHETIC RESIN-PRODUCING INDUSTRIES"", 《CHEMOSPHERE》 * |
| 卞华松 等: "冷冻固定化优势菌群处理含甲醛苯酚废水", 《环境科学》 * |
| 贺琳 等: "废水生物处理中固定化微生物技术方法与载体使用", 《内蒙古环境科学》 * |
| 魏思宇: "甲醛废水前处理方法的选择", 《中国优秀硕士论文全文数据库.工程科技I辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531666A (en) * | 2014-08-29 | 2015-04-22 | 华南理工大学 | Preparation method and application of kaolin immobilized GY2B bacterium |
| CN111218405A (en) * | 2018-11-27 | 2020-06-02 | 成功大学 | Method for producing paecilomyces rhododendron mycelium, bioplastic, and wastewater treatment method |
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