CN102016042A - 具有磷脂酶抑制活性的分离的肽 - Google Patents
具有磷脂酶抑制活性的分离的肽 Download PDFInfo
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Abstract
本发明提供了来自具有磷脂酶抑制活性的脂肪酶的C-端肽,包含磷脂酶抑制活性的多肽和能够受包含磷脂酶抑制活性的分离的肽和/或多肽抑制的脂肪酶。本发明亦涉及包含所述多核苷酸的核酸构建体、重组表达载体和重组宿主细胞,以及产生并使用具有脂肪酶抑制活性的肽和多肽的方法。
Description
涉及序列表
本申请含有计算机可读形式的序列表。将所述计算机可读形式通过提述并入本文。
发明领域
本发明涉及脂肪酶领域。具体而言,其涉及具有磷脂酶抑制活性的分离的肽和编码该肽的分离多核苷酸。本发明亦涉及包含所述多核苷酸的核酸构建体、载体和宿主细胞以及产生和使用该肽的方法。其另外涉及具有磷脂酶抑制活性的多肽和能够受上述肽和/或多肽抑制的脂肪酶。
发明背景
脂肪酶(EC3.1.1.3)水解三酰甘油的酯键并在非水系统中催化酯化和酯交换(酸解、醇解和相互酯化)。一些脂肪酶,如磷脂酶,水解磷脂中的酯键,所述磷脂为对于细胞膜结构和功能是极为重要的,且为膜脂质中最丰富的极性脂质。Nagao等,1998,J.Biochem.124:1124-29描述了异孢镰孢(Fusarium heterosporum)磷脂酶的26个氨基酸C-末端肽在增加所述脂肪酶的热稳定性中起重要作用,且该肽对酶活性无影响。
考虑到脂质的广泛用途,例如,作为消化剂,用于在面团和面团焙烤产物中产生味道;作为治疗剂,洗涤剂的成分,作为光学解析(optical resolution)的催化剂等,期望有控制脂肪酶酶活性的手段。此外,从生产的角度而言,期望控制脂肪分解活性,因为可选择制备其酶活性可再现的酶产物。
发明内容
本发明的目标是提供具有脂肪酶抑制活性的肽和编码该肽的多核苷酸,以及包含所述多核苷酸的核酸构建体、载体和宿主细胞和产生并使用所述肽的方法。本发明的目标另外还在于提供具有脂肪酶抑制活性的多肽和能够受上述肽和/或多肽抑制的脂肪酶。
本发明涉及具有磷脂酶抑制活性的分离的肽,选自:(a)分离的肽,包含与SEQ ID NO:1的289-310残基或SEQ ID NO:9的154-175残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的氨基酸序列;(b)由多核苷酸编码的分离的肽,所述多核苷酸在中等严格条件或高严格条件下与SEQ ID NO:1的肽编码序列或所述SEQ ID NO:1的肽编码序列的互补链杂交;(c)由多核苷酸编码的分离的肽,所述多核苷酸包含与SEQ ID NO:1的289-310残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的核苷酸序列;或(d)包含具有下列氨基酸序列的基序的分离的肽:M1T2D3X4X5L6E7X8K9L10N11X12Y13V14X15X16D17X18E19Y20X21K22,其中X4、X5、X8、X12、X15、X16、X18和X21独立地可为任何氨基酸,其中该肽的大小小于60个氨基酸(aa)。
本发明亦涉及包含所述肽和脂肪酶的多肽,其中所述脂肪酶在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg磷脂酶活性,和/或不显示磷脂酶活性。
本发明亦涉及具有磷脂酶抑制活性的多肽,其中具有α-螺旋的至少三个溶剂可接近的(solvent acessible)残基位于其表面的亲本蛋白质在该α-螺旋中在对应于位置D3、L6、L10、Y13、V14、D17和X21的至少一个溶剂可接近的残基处和/或对应于该基序的位置E7、K9、N11、X18和Y20边缘残基处经修饰。
本发明亦涉及分离的多核苷酸、核酸构建体、重组表达载体、包含所述肽或多肽的重组宿主细胞,以及产生具有磷脂酶抑制活性的分离的肽或多肽的方法。
本发明亦涉及将所述肽或多肽结合于所述脂肪酶时,所述肽或所述多肽用于抑制脂肪酶的酶活性的用途。
本发明亦涉及具有在平板测定法中磷脂酶活性低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg和/或不显示磷脂酶活性的脂肪酶,其能够受所述肽抑制,其中所述脂肪酶包含至少一个改变,所述改变独立地为插入、缺失或取代,由此在结合(a)具有磷脂酶抑制活性的分离的肽;或(b)在所述多肽中包含的具有磷脂酶抑制活性的肽中至少之一时所述脂肪酶的活性受到抑制。
附图简述
图1显示尖镰孢(F.oxysporum)磷脂酶的序列比对。
图2显示α-β和全α蛋白质序列。
图3显示来自尖镰孢α螺旋的残基的溶剂可接近性(so1vent accessiblility)。
图4显示GZEL的晶体。
图5显示GZEL晶体的代表性衍射图样。
图6显示FoL PI的肽诱导改变。
图7显示莫尼糖蛋白(Monellin)变体1(即MON1)和莫尼糖蛋白变体2(即MON2)序列。
图8显示对于多种肽的初始反应速率的变化。
图9显示对于多种肽和脂肪酶的%抑制的变化。
序列表
SEQ ID NO:1:尖镰孢,FoL
SEQ ID NO:2:禾谷镰孢(Fusarium Graminearium)
SEQ ID NO:3:丛赤壳属(Nectria)脂肪酶1
SEQ ID NO:4:丛赤壳属脂肪酶2
SEQ ID NO:5:异孢镰孢
SEQ ID NO:6:半裸镰孢(Fusarium semitectum)
SEQ ID NO:7:腐皮镰孢(Fusarium solani)LipC
SEQ ID NO:8:腐皮镰孢LipD
SEQ ID NO:9:细毛嗜热霉(Thermomyces lanuginosus),TLL
SEQ ID NO:10:镶片镰孢(Fusarium venenatum)PLA2,FVPLA2
SEQ ID NO:11:菌丝霉素(plectasin)
SEQ ID NO:12:莫尼糖蛋白
SEQ ID NO:13:Protegrin
SEQ ID NO:14:芽孢杆菌RNA酶(Barnase)
SEQ ID NO:15:胱蛋白(Cystatins)
SEQ ID NO:16:载脂蛋白E
定义
磷脂酶活性:术语“磷脂酶活性”在本文中定义为磷脂分解(EC号3.1.1.4)活性,其将磷脂转化为脂肪酸和其他亲脂性物质。就本发明而言,磷脂酶活性根据在下文段落“材料和方法”中描述的PHLU和平板测定法的步骤来确定。
磷脂酶抑制活性:术语“磷脂酶抑制活性”在本文中定义为抑制磷脂酶活性的活性。本发明的肽具有SEQ ID NO:1的成熟多肽的至少20%,至少40%,至少50%,至少60%,至少70%,至少80%,至少90%,至少95%或100%的磷脂酶抑制活性。
分离的肽/多肽:术语“分离的肽”或“分离的多肽”在本文中分别指自源(source)分离的肽或多肽。在某些方面,所述肽/多肽如通过SDS-PAGE或HPLC所确定的为至少1%纯,至少5%纯,至少10%纯,至少20%纯,至少40%纯,至少60%纯,至少80%纯或至少90%纯。肽纯度通过HPLC确定。
同一性:两个氨基酸序列之间或两个核苷酸序列之间的相关性由参数“同一性”来描述。就本发明而言,两个氨基酸序列之间的同一性程度是使用如EMBOSS程序包(EMBOSS:The European Molecular Biology Open Software Suite,Rice等,2000,Trends in Genetics 16:276-277)(优选版本3.0.0或更新的版本)的Needle程序中执行的Needleman-Wunsch算法(Needleman和Wunsch,1970,J.Mol.Biol.48:443-453)来确定的。使用的可选参数是:缺口开放罚分为10,缺口延伸罚分为0.5,和EBLOSUM62(BLOSUM62的EMBOSS版本)取代矩阵。将标记为“最长同一性”的Needle输出(使用-nobrief选项获得)用作百分比同一性并且是如下计算的:(相同的残基x100)/(比对的长度-比对中的缺口总数)。就本发明而言,两个脱氧核糖核苷酸序列之间的同一性程度使用如EMBOSS程序包(EMBOSS:The European Molecular Biology Open Software Suite,Rice等,2000,见上)(优选版本3.0.0或更新的版本)的Needle程序中执行的Needleman-Wunsch算法(Needleman和Wunsch,1970,见上)确定。使用的可选参数是:缺口开放罚分为10,缺口延伸罚分为0.5,和EDNAFULL(NCBI NUC4.4的EMBOSS版本)取代矩阵。将标记为“最长同一性”的Needle输出(使用-nobrief选项获得)用作百分比同一性并且是如下计算的:(相同的脱氧核糖核苷酸x100)/(比对的长度-比对中的缺口总数)。
同源序列:术语“同源序列”在本文中定义为在tfasty检索(Pearson,W.R.,1999,于Bioinformatics Methods and Protocols,S.Misener和S.A.Krawetz编,185-219页)中给出小于0.001的E值(或预期分数)的预测蛋白质。同源性的程度可适当的通过本领域已知的计算机程序来确定,如GCG程序包中提供的GAP(Program Manual for the Wisconsin Package,Version 8,August 1994,Genetics Computer Group,575Science Drive,Madison,Wisconsin,USA 53711)(Needleman,S.B.和Wunsch,C.D.,(1970),Journal of Molecular Biology,48,443-45)。在本发明中,禾谷镰孢、丛赤壳属脂肪酶、腐皮镰孢、半裸镰孢、尖镰孢、异孢镰孢和细毛嗜热霉(同物异名:疏棉状腐质霉(Humicola lanuginose))的脂肪酶序列的相应(或同源)位置由图1所示的比对定义。为了寻找未显示于比对中的脂肪酶序列的同源位置,将目标序列和图1所示的序列进行比对。将新序列与图1的现有比对通过使用由所述GAP程序所找到的最具同源性序列的GAP比对来进行比对。对于多肽序列比较使用下述设定:GAP产生罚分(GAP creation penalty)为3.0而GAP延伸罚分(GAP extension penalty)为0.1。
分离的多核苷酸:术语“分离的多核苷酸”用于本文中指从来源分离的多核苷酸。在某些方面,如通过琼脂糖电泳测定的,所述多核苷酸为至少1%纯,至少5%纯,至少10%纯,至少20%纯,至少40%纯,至少60%纯,至少80%纯或至少90%纯。
编码序列:当用于本文时术语“编码序列”的意思是直接指定其蛋白产物的氨基酸序列的核苷酸序列。编码序列的边界通常由开读框决定,所述开读框通常以ATG起始密码子或可供选择的起始密码子如GTG和TTG开始,并且以终止密码子如TAA、TAG和TGA结束。编码序列可以是DNA、cDNA、合成的或重组的核苷酸序列。
核酸构建体:术语“核酸构建体”用于本文指单链或双链的核酸分子,所述核酸分子分离自天然存在的基因,或将所述核酸分子以本来不存在于(not otherwise exist)自然界中的方式修饰以含有核酸的区段或所述核酸分子是合成的。当所述核酸构建体含有表达本发明的编码序列所需的控制序列时,术语核酸构建体与术语“表达盒”同义。
控制序列(control sequence):术语“控制序列”在本文定义为包括对编码本发明多肽的多核苷酸的表达是必需的所有组分。各个控制序列对于编码所述多肽的核苷酸序列可以是天然的或外源的,或各个控制序列对于彼此可以是天然的或外源的。这些控制序列包括但不限于前导序列、聚腺苷酸化序列、前肽序列、启动子、信号肽序列和转录终止子。最少的情况,控制序列包括启动子和转录和翻译的终止信号。控制序列可以和接头一起提供,所述接头目的为引入特异性限制位点的,所述特异性限制位点促进控制序列与编码多肽的核苷酸序列的编码区连接。
可操作地连接:术语“可操作地连接”在本文表示这样的构型,其中将控制序列置于相对于多核苷酸序列的编码序列的适当位置,使得控制序列指导多肽编码序列的表达。
表达:术语“表达”包括涉及多肽产生的任何步骤,其包括但不限于转录、转录后修饰、翻译、翻译后修饰和分泌。
表达载体:术语“表达载体”在本文定义为线性的或环状的DNA分子,其包含编码本发明多肽的多核苷酸,并且所述多核苷酸与提供用于其表达的额外核苷酸可操作地连接。
宿主细胞:如本文中所使用的术语“宿主细胞”包括任何细胞类型,所述细胞类型对于使用包含本发明多核苷酸的核酸构建体或表达载体的转化、转染、转导等是易感的(susceptible)。
改变:术语“改变”在本文的意思是,对由SEQ ID NO:1的成熟多肽组成的多肽或其同源序列的任何化学改变,以及对编码这样的多肽的DNA的遗传操作。所述改变可以是一个或多个(几个)氨基酸的取代、缺失和/或插入,以及一个或多个(几个)氨基酸侧链的置换。为了易于参考,在描述本发明的经修饰的氨基酸序列时,采用了以下命名法:原始氨基酸:位置:取代氨基酸。根据这种命名法,例如,在位置195中用谷氨酸(E)取代甘氨酸(G)表示为G195E。在相同的位置中缺失甘氨酸表示为G195*,而插入额外的氨基酸残基例如赖氨酸(K)表示为G195GK。与其它脂肪酶相比,当特定脂肪酶含有“缺失”并且在该位置进行插入的情况,就在位置36插入天冬氨酸(D)而言,将这种情况表示为*36D。用加号分隔多个突变,即:R170Y+G195E,分别表示在位置170用酪氨酸(Y)取代精氨酸(R),和在位置195用谷氨酸(E)取代甘氨酸(G)。当应用所述比对方法时,X231表示在亲本多肽中对应于位置231的氨基酸。X231R表示用R置换所述氨基酸。就SEQ ID NO:2而言,X是T,因此X231R表示用R取代位置231的T。当某个位置(例如231)中的氨基酸可以由选自一组氨基酸(例如由R和P和Y组成的组)的另一个氨基酸取代时,将这种情况表示为X231R/P/Y。在所有的情况下,采用公认的IUPAC单字母或三字母的氨基酸缩写。
一个给定的氨基酸残基是否在所述酶的表面可根据W.Kabsch和C.Sander(1983).Biopolymers 22,2577-2637页.Dictionary of protein secondary structure:pattern recognition of hydrogen-bonded and geometrical features确定,例如当其溶剂可接近的表面用DSSP程序计算超过30时。
发明详述
本发明人发现了一种肽,其可自尖镰孢或禾谷镰孢(玉米赤霉(Gibberella zeae))的C-端分离,并能够结合所述脂肪酶。他们另外还显示了尖镰孢的肽具有磷脂酶抑制活性。因此表明自任何合适的磷脂酶,例如,丛赤壳属脂肪酶、红球丛赤壳(Nectria haematococca)(真马特镰孢(Fusarium eumartii))、腐皮镰孢、大刀镰孢(Fusarium culmorum)、半裸镰孢和异孢镰孢分离的类似的C-端肽,可用于抑制磷脂酶活性。
在第一方面,本发明涉及具有磷脂酶抑制活性的分离的肽,选自:(a)分离的肽,包含与SEQ ID NO:1的289-310残基或SEQ ID NO:9的154-175残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的氨基酸序列;(b)由多核苷酸编码的分离的肽,所述多核苷酸在中等严格条件或高严格条件下与SEQ ID NO:1的肽编码序列或所述SEQ ID NO:1的肽编码序列的互补链杂交;(c)由多核苷酸编码的分离的肽,所述多核苷酸包含与SEQ ID NO:1的289-310残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的核苷酸序列;或(d)包含具有下列氨基酸序列的基序的分离的肽:M1T2D3X4X5L6E7X8K9L10N11X12Y13V14X15X16D17X18E19Y20X21K22,其中X4、X5、X8、X12、X15、X16、X18和X21独立地可为任何氨基酸,其中该肽的大小小于60个氨基酸(aa)。
在另一方面,本发明涉及具有长度少于55aa,少于50aa,少于45aa,少于40aa,少于35aa或少于30aa的肽。
在另一方面,本发明涉及具有长度至少15aa,至少20aa或至少25aa的肽。
在另一方面,本发明涉及这样的肽,其中所述肽具有α-螺旋的二级结构。
在另一方面,本发明涉及这样的肽,其中在基序中存在的X4、X5、X8、X12、X15、X16、X18和X21位置中每一个处的氨基酸独立选取,使得X4为A或E;X5为E或Q;X8为K或A;X12为S或N;X15为E、Q或A;X16为L或M;X18为K或Q;而X21为I或V。
尖镰孢脂肪酶(FoL)的肽可经改变以改进或减少所述肽抑制所述脂肪酶的能力。上述改变的实例示于表1。
表1:在来源于尖镰孢脂肪酶的,具有磷脂酶抑制活性的肽中,对应于SEQ ID NO:1的残基317-346处的改变,以通过改变的肽获得对野生型脂肪酶改进的或减少的抑制。
原始氨基酸 | 改进的抑制 | 减少的抑制 |
D291 | D291E | |
L294 | L294A | |
E295 | E295T,S | |
K297 | K297R | |
L298 | L298A | |
N299 | N299D,E | |
Y301 | Y301W | |
D305 | D305A | |
K306 | K306R | |
Y308 | Y308E,D | |
V309 | V309I,N,Q |
在另一方面,本发明涉及这样的肽,其与SEQ ID NO:1相比在对应于FoL残基D291;L294;E295;K297;L298;N299;Y301;D305;K306;Y308;或V309的位置处包含至少一个氨基酸取代。
在另一方面,本发明涉及这样的肽,其与SEQ ID NO:1相比在对应FoL残基D291E;L294A;E295T,S;K297R;L298A;N299D,E;Y301W;D305A;K306R;Y308E,D;或V309I,N,Q处包含至少一个氨基酸取代。
期望所述肽中的改变对肽和脂肪酶之间的结合在一些情况下可导致变化而在另一些情况下导致无变化。变化可导致改进的或减少的结合,其相应地可导致所述肽对所述脂肪酶改进的或减少的抑制。
除了所述肽中的改变外,所述肽和所述脂肪酶之间的相互作用亦受到所述脂肪酶中的改变影响。上述改变可有助于改进的抑制或减少的抑制。尖镰孢脂肪酶的氨基酸序列中改变的实例示于表2。
表2:尖镰孢脂肪酶中对应于SEQ ID NO:1的残基1-316处的改变,以获得所述野生型肽对改变的脂肪酶的改进的或减少的抑制。
原始氨基酸 | 改进的抑制 | 减少的抑制 |
L92 | L92D,E | L92W |
R96 | R96E,D,A | |
L203 | L203W,K,M | |
I207 | I207D,E | |
R211 | R211H | |
L243 | L243W,K | |
L250 | L250D,E | L250R |
L252 | L252S,T |
在另一方面,本发明涉及编码所述肽的分离的多核苷酸。
在另一方面,本发明涉及包含多核苷酸的核酸构建体,所述多核苷酸可操作连接于至少一个控制序列,该控制序列在表达载体中指导所述肽的产生。
在另一方面,本发明涉及包含所述核酸构建体的重组表达载体。
在另一方面,本发明涉及包含所述核酸构建体或所述重组表达载体的重组宿主细胞。
在另一方面,本发明涉及制备所述分离的肽的方法,包括下列步骤:(a)在有益于所述肽产生的条件下培养包含核酸构建体的宿主细胞,所述核酸构建体包含编码所述肽至少一个拷贝的多核苷酸;和(b)回收所述肽。
或者,本发明的肽可通过体外合成来制备,使用本领域已知的常规方法。可用的是多种商业合成设备,例如来自Applied Biosystems Inc.,Beckman等的自动合成仪。通过使用合成仪,天然存在的氨基酸可用非天然的氨基酸,特别是D-异构体(D-形式),例如D-丙氨酸和D-异亮氨酸,非对映异构体,具有不同长度和官能团的侧链等所取代。特定的序列和制备方式将由方便性,经济性,所需的纯度等确定。
所述肽亦可根据重组合成的常规方法分离并纯化。可制备所述表达宿主的裂解物,且所述裂解物使用HPLC,排阻层析,凝胶电泳,亲和层析或其他纯化技术纯化。大部分情况下,使用的组合物相对于与所述产物制备和其纯化方法相关的污染物,应包含按重量计至少20%的所需产物,更通常按重量计至少约75%,优选按重量计至少约95%,而就治疗目的而言,通常按重量计至少约99.5%。通常,所述百分比应基于总蛋白质。
在另一方面,本发明涉及当所述肽结合于所述脂肪酶时,所述肽用于抑制脂肪酶的酶活性的用途。
一些脂肪酶未归类为磷脂酶,且期望自不具有或几乎不具有磷脂酶活性的亲本脂肪分解酶得到具有磷脂酶活性的变体,例如,在平板测定法中对应于下述或低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性对脂肪酶活性的比例,和/或不显示磷脂酶活性。
在另一方面,本发明涉及包含所述肽和脂肪酶的多肽,其中所述脂肪酶在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性,和/或不显示磷脂酶活性。
在另一方面,本发明涉及包含脂肪酶的多肽,所述多肽与细毛嗜热霉(SEQ ID NO:9)具有至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%的同一性。
天然不包含具有抑制活性的肽的脂肪酶可经改变以供获得脂肪酶变体,其受本发明的分离的肽抑制。这些脂肪酶变体可通过(a)所述分离的肽;(b)附加于上述脂肪酶变体的肽或(c)包含具有抑制活性的肽的多肽之一所抑制。所述肽和所述脂肪酶变体之间的结合相互作用可另外通过修改所述肽的氨基酸序列来修饰。
细毛嗜热霉脂肪酶(TLL)基本上不具有磷脂酶活性,且现在并无信息表明该脂肪酶包含任何C-或N-端肽来控制和/或抑制其活性。该脂肪酶具有的结构使得其适于引入将使TLL易受尖镰孢脂肪酶(FoL)肽的结合,并由此抑制其脂肪分解活性的氨基酸改变。上述改变的实例公开于表3(A)。TLL和本发明分离的肽的相互作用可另外通过在所述肽中引入改变来优化。在FoLC-端肽中的改变的实例示于表3(B)。
表3:(A)细毛嗜热霉脂肪酶(TLL)中对应SEQ ID NO:9的残基1-269的改变,以产生受野生型FoL肽抑制的脂肪酶,和(B)在源自尖镰孢脂肪酶(FoL)具有磷脂酶抑制活性的肽中对应SEQ ID NO:1的残基288-317的改变,以获得TLL的改进的抑制。
期望当用FoL残基取代TLL残基时,所有的残基可被取代,或者至少一个选自TLL序列的残基可被根据下列比对选自FoL序列的对应的残基所取代:
在另一方面,本发明涉及这样的多肽,其中所述脂肪酶包含至少一个改变,其独立地为插入、缺失或取代。
在另一方面,本发明涉及这样的多肽,其中所述脂肪酶的至少一个改变包括对应于细毛嗜热霉(SEQ ID NO:9)的残基E87R、I90L、G91T、I202P、R209L、E210I或T244L的一个或多个氨基酸取代。
在另一方面,本发明涉及这样的多肽,其中所述脂肪酶包含用尖镰孢(SEQ ID NO:1)的残基246-254取代对应于细毛嗜热霉(SEQ ID NO:9)的248-255的残基,或用尖镰孢(SEQ ID NO:1)的残基247-252取代细毛嗜热霉(SEQ ID NO:9)的248-253。
在另一方面,本发明涉及具有磷脂酶抑制活性的多肽,其中具有α-螺旋至少三个溶剂可接近的残基位于其表面的蛋白质在该α-螺旋中在如前所述对应于位置D3、L6、L10、Y13、V14、D17和X21的至少一个所述溶剂可接近的残基和/或对应于本发明的基序的位置E7、K9、N11、X18和Y20边缘残基处经过修饰。
在另一方面,本发明涉及这样的多肽,其中所述蛋白质具有与菌丝霉素(SEQ ID NO:11);莫尼糖蛋白(SEQ ID NO:12);Protegrin(SEQ ID NO:13);芽孢杆菌RNA酶(SEQ ID NO:14);胱蛋白(SEQ ID NO:15);或载脂蛋白E(SEQ ID NO:16)至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%同一性的氨基酸序列。
适于生成具有磷脂酶抑制活性的多肽的蛋白质必须包含至少一个暴露于所述蛋白质表面的α-螺旋。包含于所述α-螺旋中的一些氨基酸需为溶剂可接近的,从而能够结合脂肪酶并发挥其抑制作用。所述α-螺旋的大小可为22氨基酸或20、18、16、14、12或10个氨基酸。除了所述至少一个α-螺旋外,可存在其他二级结构元件,如β-链。基于其二级结构,可定义三个主要的蛋白质组:全-α组,全β-组和α-β组。图2显示了属于α-β组的蛋白质的实例,如菌丝霉素、莫尼糖蛋白、protegrin、芽孢杆菌RNA酶和胱蛋白,以及属于全-α组的蛋白质的实例,如载脂蛋白E。氨基酸序列示于第二行,而第一行表示如由程序Dictionary of Secondary Structure Prediction(DSSP)计算的二级结构,其中a/A表示α-螺旋而b/B表示β-链。
可将所述磷脂酶抑制肽的α-螺旋与所选蛋白质(加下划线)的α-螺旋在两步步骤中进行重叠(superimpose)和比对,如下面对于莫尼糖蛋白的说明:
选择平行或者反平行待比对序列的方向,
-------------------->磷脂酶抑制肽
-------------------->莫尼糖蛋白(平行)
<-------------------莫尼糖蛋白(反平行)
一旦选择了方向,有几种比对α-螺旋序列的可能性。最优的比对包含最大的共同长度以及最小数目的瑕疵(trouble)(冲突,clash)。
------------------->磷脂酶抑制肽
------------------->莫尼糖蛋白
------------------->莫尼糖蛋白
------------------->莫尼糖蛋白
在鉴定出了最优比对后,在所述蛋白质α-螺旋中进行修饰以将氨基酸残基改变为所述磷脂酶抑制肽的α-螺旋。在图3中显示了一个实例,其中画出Fol肽以说明所述α-螺旋的哪一部分和哪些氨基酸残基面向所述酶,以及哪些面向溶剂。具体而言,必须考虑这样的残基,其面向框架蛋白的表面,为溶剂可接近的,并相应地参与对脂肪酶的接触以及抑制。这些残基对应于基序M1T2D3X4X5L6E7X8K9L10N11X12Y13V14X15X16D17X18E19Y20X21K22的位置D3、L6、L10、Y13、V14、D17和X21。可选地,亦应当考虑这样的残基,其见于所述蛋白表面边缘,潜在地为溶剂可接近的,并相应地可参与对脂肪酶的接触和抑制。这些残基对应于所述基序的位置E7、K9、N11、X18和Y20。
最后,分析了所述蛋白质中α-螺旋以外的部分以鉴定是否有其他和/或新的冲突,其可影响所述框架蛋白的α-螺旋与所述脂肪酶的结合或影响所述蛋白质内α-螺旋的容纳(accommodation)。随之可选地改变上述残基以最大化所述磷脂酶抑制作用。其为螺旋外的突变。
在另一方面,本发明涉及制备本发明多肽的方法,包括将所述肽附加于脂肪酶的步骤,其中所述脂肪酶在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg磷脂酶活性,和/或不显示任何磷脂酶活性。
在另一方面,本发明涉及制备多肽的方法,包括下述步骤:(a)选择在蛋白质表面具有α-螺旋的蛋白质,由此所述α-螺旋的至少三个,至少四个,至少五个或至少六个残基是溶剂可接近的;(b)将所述蛋白质的α-螺旋与所述肽进行比对;(c)鉴定出所述蛋白质的α-螺旋中溶剂可接近的残基,其对应于所述基序的位置D3、L6、L10、Y13、V14、D17和X21;(d)在步骤(c)中鉴定出的蛋白质中改变至少一个,至少两个,至少三个,至少四个,至少五个,至少六个或至少七个氨基酸;(e)测试所述多肽的磷脂酶抑制活性;(f)选择具有磷脂酶抑制活性的多肽;和(g)产生(f)中选出的多肽。
在另一方面,本发明涉及在步骤(c)中另外还鉴定出所述蛋白质的α-螺旋中的残基的方法,所述残基潜在地为溶剂可接近的,对应于所述基序的位置E7、K9、N11、X18和Y20。
在另一方面,本发明涉及这样的方法,其另外还包括在所述蛋白质中一个或多个位置进行至少一个改变的步骤。
在另一方面,本发明涉及将包含于所述多肽中的肽结合于脂肪酶时,所述多肽用于抑制所述脂肪酶的酶活性的用途。
在另一方面,本发明涉及在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性,和/或不显示任何磷脂酶活性的脂肪酶,其能够受所述肽抑制,其中所述脂肪酶包含至少一个改变,其独立地为插入、缺失或取代,由此在结合(a)具有磷脂酶抑制活性的分离的肽;或(b)包含于所述多肽的具有磷脂酶抑制活性的肽至少之一时,所述脂肪酶的活性受到抑制。
在另一方面,本发明涉及这样的脂肪酶,其中所述脂肪酶与细毛嗜热霉(SEQ ID NO:9)具有至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%的同一性。
在另一方面,本发明涉及这样的脂肪酶,其中至少一个改变是对应于细毛嗜热霉(SEQ ID NO:9)残基L92;R96;L203;I207;R211;L243;L250或L252的取代。
在另一方面,本发明涉及这样的脂肪酶,其中至少一个改变是对应于细毛嗜热霉(SEQ ID NO:9)残基L92D,E,W;R96E,D,A;L203W,K,M;I207D,E;R211H;L243W,K;L250D,E,R和L252S,T的取代。
材料和方法
磷脂酶活性(PHLU)
测量游离脂肪酸自卵磷脂的释放作为磷脂酶活性(PHLU)。向50μl在50mM HEPES,pH7中的4%L-α-磷脂酰胆碱(来自Avanti的植物卵磷脂),5mMCaCl2添加50μl在50mM HEPES,pH7中稀释至合适浓度的酶溶液。将试样在30℃温育10分钟,且所述反应在95℃停止5分钟,然后离心(在7000rpm5分钟)。使用来自Wako Chemicals GmbH的NEFA C试剂盒确定游离的脂肪酸;向25μl反应混合物添加250μl的Reagent A(试剂A),并在37℃温育10分钟。然后添加500μl Reagent B(试剂B),并将试样在37℃再次温育10分钟。使用HP8452A二极管阵列分光光度计测定550nm处的吸收。试样至少重复运行两次。包含底物和酶的盲试样(blind)(经预热的酶试样(在95℃10分钟)+底物)。使用油酸作为脂肪酸标样。1PHLU等于能够在该条件下每分钟释放1μmol游离脂肪酸的酶量。磷脂酶比活性(PHLU/mg)以每蛋白量(mg)的磷脂酶活性(PHLU)计算。
平板测定法:将50ml 2%琼脂糖通过在纯水中加热5分钟来溶解并随后冷却至60-63℃。50ml 2%植物L-α-磷脂酰胆碱95%(于0.2M NaOAc,10mMCaCl2,pH 5.5在60℃30分钟)以ultrathorax混合15秒。将等体积的2%琼脂糖和2%卵磷脂混合。添加250μl在纯水中的4mg/ml结晶紫作为指示剂。将化合物倾倒到适当的培养皿(例如,30ml于14cm皿)中,并在琼脂上制出适当的孔(3-5mm)以供施加酶溶液。将酶溶液稀释至对应于OD280=0.5和10微升的浓度,并施加于琼脂糖/卵磷脂-基质内的孔中。将平板在30℃温育,并在大约4-5小时和/或大约20小时温育后在平板中鉴定出反应区域。将疏棉状腐质霉脂肪酶用作对照,且将与对照相比较大的清亮区(clearing zone)的存在作为磷脂酶活性的阳性结果。
实施例
通过如下实施例进一步描述本发明,所述实施例不应理解为对本发明的范围的限制。
实施例1-GZEL晶体结构
蛋白质表达和纯化:玉米赤霉/禾谷镰孢(Fusarium graminearum)脂肪酶(GZEL)基因通过PCR扩增,并通过测序进一步确认。而所述GZEL基因在酵母中表达,在考马司染色后在SDS-PAGE上显示出显著的蛋白质条带。且在pH7针对橄榄油/Bright Green(亮绿)平板检测出活性。阳性候选克隆在孔周围显示深绿色的区域。
通过离心自细胞分离的组织上清,并将上清的pH调节至pH7.0。然后将上清过滤并与0.3M NaCl加载到用25mM Tris-HCl,pH7.0平衡的Ni-Sepharose FF上。用咪唑以0M到1M的梯度洗脱目标蛋白。分析来自柱的级分的活性。汇集并浓缩具有酶活性的级分。然后将试样与0.15M NaCl加载到用25mM Tris-HCl,pH8.0平衡的凝胶过滤柱Superdex 75上。浓缩洗脱的活性脂肪酶并用25mM Tris-HCl在pH8.0渗析。用SDS-PAGE检查脂肪酶,并制备纯级分以供结晶试验。
结晶:将新鲜制备的蛋白质浓缩至10mg/ml并通过在291K的悬滴蒸汽扩散(hanging drop vapor diffusion)方法来结晶。使用几种Crystal Screen Kit(结晶筛选套盒)(Hampton Research筛选套盒1和2,Index筛选套盒,MembFac筛选套盒)来筛选初始结晶条件。将一微升的蛋白质溶液与1微升的储库溶液(reservoir solution)混合,并针对200微升的储库溶液平衡。可在多种不同条件下在三日内发现小晶体。所述晶体大部分为空心的棒状物,并具有不良的衍射性能。精确成形和良好性能的晶体选自在2-4日内用0.2M硫酸铵,0.1M Bis-Tris(pH5.5),25%w/v PEG3350的条件(参见图3),其可达到它们约50*50*200微米的最终体积,其空间群为P212121。
数据收集和处理:在100K从单独的GZEL衍生物晶体使用内部的Rigaku MM-007发生器(generator)和Mar345dtb检测器收集2.8分辨率组的衍射数据组。通过锇镜(osmic mirror)聚焦光束。对于更加详细的分析,使用骤冷的(flash-cooled)晶体。将晶体浸于冷冻保护剂中5-10秒,用接种环(loop)挑取,并在冷却至100K的氮气流中骤冷。所述冷冻保护剂通过将25%甘油添加到母液储库中来制备。晶形属于空间群P212121(a=78.4,b=91.0,c=195.8,α=β=γ=90°),在每个不对称单元中有四个GZEL分子,并具有2.63Da-1的VM (Matthews 1968),对应于48%的溶剂含量。衍射图像的处理和整合强度(integrated intensity)的定标(scaling)使用HKL2000软件包(Otwinowski等,1997)进行。
结果:起初,虽然我们在所述筛选套盒的多种条件下获得了许多GZEL晶体,但它们不适于X射线衍射。许多晶体是中空纤维。因此,进行了进一步的结晶优化,并在下述条件下获得了较好的晶体:0.2M硫酸铵,0.1MBis-Tris,pH5.5,25%w/v PEG3350。将含有2μl蛋白质溶液和2μl储库溶液的滴针对200微升储库溶液平衡。从经优化的储库溶液中生长出的晶体(0.2M硫酸铵,0.1M Bis-Tris,pH5.5,25%w/v PEG3350)更加适于X射线衍射,并衍射至2.8随后从一个单独晶体(参见显示GZEL晶体d典型衍射图样的图4。暴露时间为300秒,检测器距离为150毫米而每帧的振动范围为1°)收集一组数据。每个不对称单位有四个分子。完整的数据收集统计在表4中给出。确定了GZEL的结构并在附录1中公开。
参考文献:Matthews,B.W.,Solvent content of protein crystals.J.Mol.Biol.,1968.33:p.491-497.Otwinowski,Z.和W.Minor,Processing of X-ray diffraction data collected in oscillation mode,于Macromolecular Crystallography,part A,C.W.Carter Jr.和R.M.Sweet编.1997,Academic Press.307-326页。
表4:数据收集和处理统计。R合并=100∑|Ii-<I>|/∑Ii,其中Ii是观察的强度。
实施例2-结合GZEL的肽
在所述晶体结构的不对称单元中有四个GZEL-肽复合物。所述四个复合物彼此独立进行细化(refine)。它们构成四个不同的实体(entity)。在所述四个不同的复合物中,所述肽相对脂肪酶核心域坐落在完全相同的位置,参见下面的表5。这提供了所述肽与脂肪酶核心特异性结合的另一组证据。
表5:最优重叠时GZEL-肽C_α均方根偏差。对四个GZEL-肽配对的每一个使用内部程序设计的最优重叠的两组蛋白质原子坐标。表中的条目用埃(Angstrom)显示在重叠时C_α原子的残余值(residual)(均方根偏差)。
A | B | C | D | |
A | 0.00 | 0.44 | 0.52 | 0.51 |
B | 0.44 | 0.00 | 0.49 | 0.47 |
C | 0.52 | 0.49 | 0.00 | 0.48 |
D | 0.51 | 0.47 | 0.48 | 0.00 |
实施例3:制备不含肽的FoL以供结合实验
从Pilot(中试)发酵LVF57UF浓缩物PPW6523纯化FoL。将PPW6523以0.22μm过滤,并加载至丁基-Sepharose速流(fast flow)柱上,用1.8M乙酸铵洗涤,并用MilliQ水洗脱。纯化FoL的数据表:2003-04317-01。在的等电聚焦凝胶IEF pH 3-10(Novex预制)20070628上,试样给出单一条带。所述条带的N-端测序显示出仅仅一个序列-所期待的FoL N-端,显示C-端肽未结合。
实施例4:抑制测定法
这些实验所用的底物为购自Sigma Aldrich的对硝基苯(p-NP)丁酸(酯)。通过将18μL p-NP丁酸(酯)添加至1ml 2-丙醇以获得100mM的母液来制备底物的母液。通过将10μL母液稀释于终体积1ml的50mM Tris pH7缓冲液来制备工作溶液。将45μL在50mM Tris缓冲液pH7中的1mg/ml FoL用两个浓度的所述肽预温育,且对照使用相同的缓冲液在不存在肽的条件下进行。在室温预温育5分钟后,将酶溶液在50mM Tris pH 7缓冲液中稀释,并将100μL稀释的酶溶液与100μL底物溶液混合,并在微滴定板分光光度计中在室温在恒定振荡下跟踪反应。下面的表6显示了在A405处监测的对硝基苯酚随时间的显色(每30秒,进行10分钟),所述对硝基苯酚为p-NP丁酸(酯)的一个降解产物,由酶所释放,所述酶以两个不同浓度与所述肽和不存在肽但是以完全相同方式处理的对照预温育。
表6:具有与不具有肽的FoL酶的活性数据。FoL的活性说明添加所述肽导致活性的抑制,确定为显色减少。具有肽的FoL给出最低的显色,而具有5x稀释的肽的FoL具有较高的显色,而对照试样,具有水的FoL具有最高的显色。
实施例5-等电点测定
将在实施例4中制备的试样加载到Nowex凝胶IEF3-10上。泳道1:标记物。泳道2:试样1(10μL)。泳道3:试样1(20μL)。泳道4:试样2(10μL)。泳道5:试样2(20μL)。泳道6:试样3(10μL)。泳道7:试样3(20μL)。凝胶的照片示于图6。所述凝胶揭示添加所述肽导致pI的变化,因为条带(泳道6和7)与具有肽的试样(泳道2-5)相比具有较低的pI。这显然表明所述肽与所述酶的结合。
实施例6-用卵磷脂平板测定法测定TLL和FoL的活性
如材料和方法中所述制备了不含Triton-X 100的卵磷脂平板。将基于ODA280相同量的纯化的TLL和FoL添加至孔中:TLL添加到上面两个孔,FoL添加到下面两个孔;左边的孔(较低浓度,ODA280等于0.2);右边的孔(较高浓度,ODA280等于0.5)。在20小时温育后,FoL在较低/较高浓度显示7/10mm的清亮区。TLL不显示清亮区。
表7:卵磷脂平板测定法。20小时的温育后以mm(毫米)测定的清亮区直径。基于ODA280等剂量添加酶。
ODA280 | TLL | FoL |
0.2 | 0mm | 7mm |
0.5 | 0mm | 10mm |
实施例7:如何修饰莫尼糖蛋白中的α-螺旋以生成具有磷脂酶抑制活性的多肽
最近解析的禾谷镰孢(亦称为玉米赤霉)脂肪酶结构显示通常为了酶的成熟而剪切的C-端肽存在于其结构中。该肽呈现α-螺旋结构,并填塞(pack against)了所述磷脂酶的催化域。显示所述肽抑制针对小酯的磷脂酶活性。
莫尼糖蛋白是来自African Serendipity Berry(非洲锡兰莓)的糖味蛋白(sugar tasting protein)。莫尼糖蛋白的结构由垂直填塞于5-链β-折叠的部分暴露的长α-螺旋组成。溶剂可接近的α-螺旋的存在使得所述蛋白质非常适于修饰该α-螺旋以试图转移尖镰孢脂肪酶(FoL)的C-端肽的抑制特性的目的,所述脂肪酶是禾谷镰孢脂肪酶(FGL)的接近同源物(close homologue)。设计两个不同的莫尼糖蛋白变体作为FoL抑制剂。
变体总结:在分析了将FoL的C-端肽α-螺旋重叠在莫尼糖蛋白的α-螺旋上所有可能的方法后发现了两个变体。在选择所述变体时,视觉检查(visual inspection)关注于:a)将莫尼糖蛋白α-螺旋与C-端肽残基的比对最大化,所述C-端肽残基与所述脂肪酶的催化域相互作用;和b)将莫尼糖蛋白其他部分与所述催化域的冲突最小化。鉴定出了重叠所述螺旋的两个不同可能性,或者N到C端方向匹配(平行)或将其倒转(反平行)。
序列/结构:在本研究中使用的莫尼糖蛋白的序列连同其二级结构(第一行)示于图2(第二行),所述二级结构根据由W.Kabsch和C.Sander,Biopolymers(1983)22:2577-2637公开的Dictionary of Secondary Structure Prediction(DSSP)程序计算。b/B指β-链,a/A指α-螺旋。用于重叠的RCSBPDB文件是1IV7。
变体:两个莫尼糖蛋白变体是本研究的结果。它们如下所述。
MON1变体:当所述脂肪酶肽α-螺旋与所述莫尼糖蛋白α-螺旋平行比对的重叠时,产生氨基酸取代F11E+Q13K+N14L+L15V+K17Y+F18V+N24Y+K25V+I74A+E77R+R82G+R83G。这些取代将模拟所述肽与脂肪酶催化域的相互作用,并消除了所述分子剩余部分可能的冲突。MON1的完整序列示于图6,其中被取代的氨基酸为粗体红字。可能需要将P10变为亮氨酸(P10L),但已预见到该变化将对莫尼糖蛋白的整体稳定性带来损害。
MON2变体:当所述脂肪酶肽α-螺旋与所述莫尼糖蛋白α-螺旋反平行比对的重叠时,产生氨基酸取代F11Y+Q13N+N14L+K17A+F18L+K25H+F34V+R81G+R84G。这些取代将模拟所述脂肪酶肽与脂肪酶催化域的相互作用,并消除了所述分子剩余部分可能的冲突。MON2的完整序列示于图7,其中被取代的氨基酸为粗体红字。
实施例8-不同肽对FoL野生型和FoL变体I207E的抑制作用
酶测定法:用于这些实验的底物为对硝基苯(p-NP)丁酸(酯)(N9876 Sigma Aldrich)。通过将18μL p-NP丁酸(酯)添加至1ml的2-丙醇以得到100mM母液来制备底物母液。通过将500μL母液稀释于终体积为50mL的500mM磷酸(盐),pH7,0.4%Triton X-100来制备工作溶液。将40μL在500mM磷酸(盐),pH7,0.4%Triton X-100中的38ng/mL酶与20μL具有不同浓度使得肽/脂肪酶比例为0.5到1000,以因子2递增12级的肽一同预温育。包括了含有60μL的酶底物空白。在最少预温育15分钟后,添加100μL底物并在室温起始振荡(5秒)后在微滴板分光光度计(来自Molecular Devices的SPECTRAmax PLUS384)中跟踪反应。随时间的显色在A405处监测(每20秒,持续30分钟),测定通过水解释放的对硝基苯酚,一种p-NP丁酸(酯)的降解产物。
将起始反应速率(测定为A405对时间作图的线性初始斜率)对抑制剂/脂肪酶比例的对数作图。自下述模型确定IC50值:
v0是初始反应速率,vmax是最大速率,vmin是最小反应速率而IC50是半最大抑制浓度(half maximal inhibitory concentration)。使用来自GraphPad Software,Inc.的GraphPad Prism(v.5.00,2007年3月12日)用于回归。
%抑制为初始反应速率的减少百分比,其中0%抑制定义为不含肽时的起始反应速率。
表8:测试的肽序列。HPLC纯化的(>85%纯)合成肽自TAG Copenhagen A/S获取。
名称 | 设计 | 序列 |
P0 | 野生型 | ATMTDAELEKKLNSYVQMDKEYVKNNQARS |
P1 | D291E | ATMTEAELEKKLNSYVQMDKEYVKNNQARS |
P2 | E295T | ATMTDAELTKKLNSYVQMDKEYVKNNQARS |
P3 | K297R | ATMTDAELEKRLNSYVQMDKEYVKNNQARS |
P4 | Y308E | ATMTDAELEKKLNSYVQMDKEEVKNNQARS |
P1’ | D305A | ATMTDAELEKKLNSYVQMAKEYVKNNQARS |
表9:确定的IC50值
1:IC50值(半最大抑制浓度)
2:95%置信区间
*IC50>测试的最大过量肽
结果:图8显示初始反应速率如何由不同肽浓度改变(A&B)。假定最低浓度的肽为0%抑制,根据(C),显然当对P0曲线作图时,FoL变体:I207E相比野生型酶更适于结合所述肽。
实施例9-不同肽对TLL野生型和TLL变体的抑制作用
酶测定法:如上所述。肽P11和P13在得到肽/脂肪酶比例为5到5000以因子2递增10级的浓度进行测试。
肽12在在得到肽/脂肪酶比例为6到6000以因子2递增10级的浓度进行测试。
表10:测试的肽序列
名称 | 设计 | 序列 |
P11 | L294V | ATMTDAEVEKKLNSYVQMDKEYVKNNQARS |
P12 | Q303N | ATMTDAELEKKLNSYVNMDKEYVKNNQARS |
P13 | K306A | ATMTDAELEKKLNSYVQMDAEYVKNNQARS |
表11:TLL变体N248G/Q249G/P250T/N251L/I252G/P253L的IC50值
1:IC50值(半最大抑制浓度)
2:95%置信区间
*IC50>测试的最大过量肽
结果:图9显示%抑制如何由不同肽浓度改变(肽空白为0%抑制)。
附录1
在GZEL的Protein Data Bank(PDB)格式中的坐标
ATOM 1 CB ALA 1 26.829 24.911 33.431 1.00 46.79
ATOM 2 C ALA 1 28.010 24.831 31.273 1.00 51.75
ATOM 3 O ALA 1 29.048 25.242 30.748 1.00 55.66
ATOM 4 N ALA 1 29.275 24.764 33.312 1.00 48.96
ATOM 5 CA ALA 1 28.005 24.323 32.689 1.00 49.44
ATOM 6 N VAL 2 26.838 24.818 30.659 1.00 49.20
ATOM 7 CA VAL 2 26.701 25.297 29.297 1.00 46.02
ATOM 8 CB VAL 2 26.195 24.190 28.377 1.00 44.99
ATOM 9 CG1 VAL 2 26.187 24.670 26.943 1.00 43.38
ATOM 10 CG2 VAL 2 27.093 22.967 28.534 1.00 40.91
ATOM 11 C VAL 2 25.705 26.444 29.321 1.00 45.79
ATOM 12 O VAL 2 24.830 26.501 30.192 1.00 46.48
ATOM 13 N SER 3 25.844 27.373 28.382 1.00 45.59
ATOM 14 CA SER 3 24.951 28.533 28.326 1.00 45.63
ATOM 15 CB SER 3 25.543 29.692 29.138 1.00 46.98
ATOM 16 OG SER 3 24.691 30.821 29.139 1.00 50.89
ATOM 17 C SER 3 24.738 28.971 26.886 1.00 42.84
ATOM 18 O SER 3 25.100 28.256 25.949 1.00 46.23
ATOM 19 N VAL 4 24.148 30.143 26.703 1.00 38.57
ATOM 20 CA VAL 4 23.901 30.633 25.356 1.00 37.41
ATOM 21 CB VAL 4 22.400 30.871 25.136 1.00 36.17
ATOM 22 CG1 VAL 4 21.933 32.057 25.965 1.00 34.74
ATOM 23 CG2 VAL 4 22.125 31.071 23.661 1.00 35.04
ATOM 24 C VAL 4 24.671 31.941 25.171 1.00 36.25
ATOM 25 O VAL 4 25.036 32.593 26.163 1.00 34.50
ATOM 26 N SER 5 24.935 32.322 23.918 1.00 34.33
ATOM 27 CA SER 5 25.664 33.559 23.655 1.00 34.34
ATOM 28 CB SER 5 27.076 33.268 23.179 1.00 30.08
ATOM 29 OG SER 5 27.086 32.928 21.806 1.00 23.67
ATOM 30 C SER 5 25.032 34.490 22.627 1.00 35.94
ATOM 31 O SER 5 24.066 34.149 21.921 1.00 38.21
ATOM 32 N THR 6 25.631 35.668 22.536 1.00 35.10
ATOM 33 CA THR 6 25.194 36.693 21.625 1.00 33.67
ATOM 34 CB THR 6 26.131 37.887 21.716 1.00 33.12
ATOM 35 OG1 THR 6 25.661 38.744 22.755 1.00 33.18
ATOM 36 CG2 THR 6 26.203 38.662 20.385 1.00 34.10
ATOM 37 C THR 6 25.198 36.161 20.219 1.00 34.02
ATOM 38 O THR 6 24.183 36.172 19.528 1.00 37.02
ATOM 39 N THR 7 26.360 35.692 19.801 1.00 36.77
ATOM 40 CA THR 7 26.506 35.158 18.474 1.00 37.15
ATOM 41 CB THR 7 27.972 35.059 18.119 1.00 38.00
ATOM 42 OG1 THR 7 28.138 34.070 17.104 1.00 42.94
ATOM 43 CG2 THR 7 28.796 34.694 19.333 1.00 35.45
ATOM 44 C THR 7 25.850 33.788 18.346 1.00 37.14
ATOM 45 O THR 7 25.545 33.333 17.241 1.00 35.92
ATOM 46 N ASP 8 25.655 33.121 19.479 1.00 35.56
ATOM 47 CA ASP 8 25.005 31.824 19.478 1.00 36.26
ATOM 48 CB ASP 8 25.069 31.157 20.859 1.00 37.91
ATOM 49 CG ASP 8 26.118 30.044 20.930 1.00 42.07
ATOM 50 OD1 ASP 8 26.845 29.821 19.926 1.00 42.80
ATOM 51 OD2 ASP 8 26.207 29.385 21.992 1.00 40.71
ATOM 52 C ASP 8 23.565 32.055 19.084 1.00 34.39
ATOM 53 O ASP 8 23.020 31.297 18.295 1.00 32.33
ATOM 54 N PHE 9 22.956 33.110 19.612 1.00 33.82
ATOM 55 CA PHE 9 21.576 33.396 19.262 1.00 34.15
ATOM 56 CB PHE 9 21.000 34.468 20.165 1.00 32.06
ATOM 57 CG PHE 9 19.502 34.518 20.138 1.00 31.29
ATOM 58 CD1 PHE 9 18.766 33.459 20.656 1.00 29.36
ATOM 59 CD2 PHE 9 18.827 35.611 19.581 1.00 30.22
ATOM 60 CE1 PHE 9 17.367 33.478 20.631 1.00 31.11
ATOM 61 CE2 PHE 9 17.453 35.651 19.544 1.00 31.35
ATOM 62 CZ PHE 9 16.705 34.571 20.072 1.00 34.10
ATOM 63 C PHE 9 21.463 33.865 17.817 1.00 35.20
ATOM 64 O PHE 9 20.526 33.515 17.095 1.00 37.15
ATOM 65 N GLY 10 22.430 34.674 17.406 1.00 35.16
ATOM 66 CA GLY 10 22.443 35.182 16.049 1.00 34.12
ATOM 67 C GLY 10 22.435 34.027 15.085 1.00 31.88
ATOM 68 O GLY 10 21.608 33.975 14.180 1.00 31.31
ATOM 69 N ASN 11 23.367 33.098 15.289 1.00 32.62
ATOM 70 CA ASN 11 23.474 31.915 14.446 1.00 32.09
ATOM 71 CB ASN 11 24.500 30.936 15.000 1.00 30.29
ATOM 72 CG ASN 11 25.918 31.372 14.715 1.00 27.15
ATOM 73 OD1 ASN 11 26.167 32.066 13.732 1.00 25.09
ATOM 74 ND2 ASN 11 26.855 30.960 15.560 1.00 20.33
ATOM 75 C ASN 11 22.148 31.206 14.360 1.00 32.33
ATOM 76 O ASN 11 21.715 30.830 13.262 1.00 34.04
ATOM 77 N PHE 12 21.524 31.021 15.527 1.00 30.36
ATOM 78 CA PHE 12 20.215 30.371 15.624 1.00 32.00
ATOM 79 CB PHE 12 19.626 30.516 17.051 1.00 29.79
ATOM 80 CG PHE 12 20.365 29.735 18.124 1.00 24.06
ATOM 81 CD1 PHE 12 21.479 28.953 17.813 1.00 24.09
ATOM 82 CD2 PHE 12 19.955 29.795 19.464 1.00 22.95
ATOM 83 CE1 PHE 12 22.165 28.254 18.817 1.00 20.49
ATOM 84 CE2 PHE 12 20.659 29.079 20.467 1.00 21.55
ATOM 85 CZ PHE 12 21.754 28.321 20.123 1.00 20.63
ATOM 86 C PHE 12 19.276 31.053 14.613 1.00 31.42
ATOM 87 O PHE 12 18.788 30.434 13.656 1.00 33.69
ATOM 88 N LYS 13 19.056 32.343 14.824 1.00 28.13
ATOM 89 CA LYS 13 18.178 33.097 13.968 1.00 24.06
ATOM 90 CB LYS 13 18.120 34.542 14.443 1.00 24.85
ATOM 91 CG LYS 13 17.469 34.664 15.785 1.00 26.16
ATOM 92 CD LYS 13 17.143 36.111 16.115 1.00 31.82
ATOM 93 CE LYS 13 16.040 36.686 15.214 1.00 34.72
ATOM 94 NZ LYS 13 15.847 38.173 15.401 1.00 35.98
ATOM 95 C LYS 13 18.612 33.053 12.532 1.00 21.70
ATOM 96 O LYS 13 17.809 33.258 11.617 1.00 23.96
ATOM 97 N PHE 14 19.882 32.787 12.311 1.00 22.50
ATOM 98 CA PHE 14 20.338 32.800 10.948 1.00 22.03
ATOM 99 CB PHE 14 21.809 33.188 10.876 1.00 20.84
ATOM 100 CG PHE 14 22.375 33.054 9.508 1.00 23.35
ATOM 101 CD1 PHE 14 21.949 33.893 8.479 1.00 16.32
ATOM 102 CD2 PHE 14 23.269 32.027 9.212 1.00 25.22
ATOM 103 CE1 PHE 14 22.398 33.700 7.183 1.00 20.53
ATOM 104 CE2 PHE 14 23.722 31.836 7.895 1.00 24.23
ATOM 105 CZ PHE 14 23.286 32.668 6.892 1.00 23.06
ATOM 106 C PHE 14 20.155 31.508 10.185 1.00 20.08
ATOM 107 O PHE 14 19.757 31.528 9.014 1.00 18.56
ATOM 108 N TYR 15 20.469 30.388 10.820 1.00 22.14
ATOM 109 CA TYR 15 20.383 29.145 10.100 1.00 28.79
ATOM 110 CB TYR 15 21.181 28.078 10.854 1.00 28.20
ATOM 111 CG TYR 15 22.677 28.326 10.692 1.00 29.94
ATOM 112 CD1 TYR 15 23.498 28.492 11.792 1.00 31.99
ATOM 113 CE1 TYR 15 24.884 28.755 11.653 1.00 32.66
ATOM 114 CD2 TYR 15 23.259 28.430 9.428 1.00 31.55
ATOM 115 CE2 TYR 15 24.628 28.692 9.269 1.00 33.72
ATOM 116 CZ TYR 15 25.442 28.850 10.394 1.00 33.86
ATOM 117 OH TYR 15 26.807 29.062 10.294 1.00 28.91
ATOM 118 C TYR 15 18.997 28.681 9.672 1.00 30.43
ATOM 119 O TYR 15 18.874 28.025 8.639 1.00 28.40
ATOM 120 N ILE 16 17.944 29.024 10.409 1.00 30.41
ATOM 121 CA ILE 16 16.630 28.580 9.957 1.00 30.84
ATOM 122 CB ILE 16 15.471 29.079 10.830 1.00 31.23
ATOM 123 CG2 ILE 16 14.975 27.958 11.664 1.00 28.44
ATOM 124 CG1 ILE 16 15.886 30.291 11.656 1.00 32.82
ATOM 125 CD1 ILE 16 15.349 31.590 11.107 1.00 33.34
ATOM 126 C ILE 16 16.366 29.035 8.540 1.00 31.82
ATOM 127 O ILE 16 15.580 28.419 7.830 1.00 34.26
ATOM 128 N GLN 17 17.016 30.108 8.105 1.00 30.85
ATOM 129 CA GLN 17 16.775 30.575 6.742 1.00 28.53
ATOM 130 CB GLN 17 17.574 31.858 6.463 1.00 30.13
ATOM 131 CG GLN 17 17.234 32.992 7.473 1.00 30.83
ATOM 132 CD GLN 17 17.579 34.397 6.964 1.00 32.84
ATOM 133 OE1 GLN 17 17.582 35.348 7.733 1.00 28.29
ATOM 134 NE2 GLN 17 17.854 34.528 5.664 1.00 34.81
ATOM 135 C GLN 17 17.122 29.456 5.772 1.00 26.12
ATOM 136 O GLN 17 16.461 29.257 4.771 1.00 22.90
ATOM 137 N HIS 18 18.157 28.699 6.096 1.00 24.71
ATOM 138 CA HIS 18 18.554 27.574 5.260 1.00 26.07
ATOM 139 CB HIS 18 19.952 27.134 5.596 1.00 25.26
ATOM 140 CG HIS 18 20.996 27.922 4.894 1.00 26.41
ATOM 141 CD2 HIS 18 21.394 27.907 3.603 1.00 25.37
ATOM 142 ND1 HIS 18 21.798 28.835 5.538 1.00 28.88
ATOM 143 CE1 HIS 18 22.659 29.344 4.672 1.00 29.96
ATOM 144 NE2 HIS 18 22.433 28.795 3.491 1.00 28.73
ATOM 145 C HIS 18 17.624 26.408 5.465 1.00 27.02
ATOM 146 O HIS 18 17.345 25.655 4.534 1.00 27.11
ATOM 147 N GLY 19 17.148 26.252 6.693 1.00 28.43
ATOM 148 CA GLY 19 16.217 25.178 6.943 1.00 29.45
ATOM 149 C GLY 19 14.979 25.390 6.083 1.00 31.53
ATOM 150 O GLY 19 14.518 24.461 5.417 1.00 34.43
ATOM 151 N ALA 20 14.464 26.624 6.090 1.00 28.21
ATOM 152 CA ALA 20 13.268 27.022 5.339 1.00 28.35
ATOM 153 CB ALA 20 12.785 28.396 5.826 1.00 26.16
ATOM 154 C ALA 20 13.547 27.086 3.842 1.00 29.16
ATOM 155 O ALA 20 12.654 26.891 3.004 1.00 33.64
ATOM 156 N ALA 21 14.798 27.390 3.520 1.00 27.87
ATOM 157 CA ALA 21 15.241 27.493 2.145 1.00 25.44
ATOM 158 CB ALA 21 16.668 28.040 2.104 1.00 19.43
ATOM 159 C ALA 21 15.187 26.105 1.511 1.00 26.54
ATOM 160 O ALA 21 14.754 25.954 0.365 1.00 29.36
ATOM 161 N ALA 22 15.620 25.092 2.263 1.00 27.19
ATOM 162 CA ALA 22 15.639 23.730 1.758 1.00 28.51
ATOM 163 CB ALA 22 15.989 22.789 2.858 1.00 26.37
ATOM 164 C ALA 22 14.276 23.376 1.184 1.00 32.45
ATOM 165 O ALA 22 14.164 22.491 0.317 1.00 33.17
ATOM 166 N TYR 23 13.250 24.079 1.669 1.00 34.80
ATOM 167 CA TYR 23 11.878 23.856 1.240 1.00 35.46
ATOM 168 CB TYR 23 10.900 24.500 2.230 1.00 31.16
ATOM 169 CG TYR 23 10.567 23.607 3.423 1.00 31.27
ATOM 170 CD1 TYR 23 9.927 22.370 3.254 1.00 29.95
ATOM 171 CE1 TYR 23 9.624 21.533 4.367 1.00 24.00
ATOM 172 CD2 TYR 23 10.901 23.987 4.718 1.00 28.94
ATOM 173 CE2 TYR 23 10.615 23.163 5.817 1.00 27.14
ATOM 174 CZ TYR 23 9.979 21.948 5.642 1.00 24.07
ATOM 175 OH TYR 23 9.709 21.174 6.755 1.00 30.00
ATOM 176 C TYR 23 11.574 24.334 -0.171 1.00 38.29
ATOM 177 O TYR 23 10.434 24.227 -0.628 1.00 39.77
ATOM 178 N CYS 24 12.566 24.873 -0.867 1.00 40.13
ATOM 179 CA CYS 24 12.300 25.303 -2.227 1.00 43.23
ATOM 180 C CYS 24 13.517 25.301 -3.116 1.00 42.88
ATOM 181 O CYS 24 13.401 25.294 -4.330 1.00 42.12
ATOM 182 CB CYS 24 11.628 26.679 -2.240 1.00 47.63
ATOM 183 SG CYS 24 12.447 27.997 -1.288 1.00 56.39
ATOM 184 N ASN 25 14.691 25.287 -2.514 1.00 42.45
ATOM 185 CA ASN 25 15.906 25.271 -3.304 1.00 44.76
ATOM 186 CB ASN 25 16.952 26.149 -2.642 1.00 43.87
ATOM 187 CG ASN 25 16.533 27.586 -2.612 1.00 48.58
ATOM 188 OD1 ASN 25 15.624 27.974 -1.875 1.00 48.53
ATOM 189 ND2 ASN 25 17.171 28.391 -3.443 1.00 52.09
ATOM 190 C ASN 25 16.412 23.852 -3.460 1.00 45.88
ATOM 191 O ASN 25 17.475 23.613 -4.039 1.00 46.08
ATOM 192 N SER 26 15.610 22.917 -2.965 1.00 48.46
ATOM 193 CA SER 26 15.940 21.509 -2.998 1.00 49.56
ATOM 194 CB SER 26 14.972 20.760 -2.085 1.00 51.09
ATOM 195 OG SER 26 13.680 21.347 -2.136 1.00 54.46
ATOM 196 C SER 26 15.885 20.973 -4.417 1.00 48.64
ATOM 197 O SER 26 16.054 19.769 -4.651 1.00 46.39
ATOM 198 N GLU 27 15.665 21.886 -5.359 1.00 48.70
ATOM 199 CA GLU 27 15.558 21.526 -6.754 1.00 47.85
ATOM 200 CB GLU 27 14.123 21.687 -7.187 1.00 50.86
ATOM 201 CG GLU 27 13.741 20.727 -8.260 1.00 54.11
ATOM 202 CD GLU 27 13.588 19.339 -7.708 1.00 53.46
ATOM 203 OE1 GLU 27 12.499 19.046 -7.165 1.00 48.31
ATOM 204 OE2 GLU 27 14.565 18.558 -7.799 1.00 55.78
ATOM 205 C GLU 27 16.426 22.410 -7.623 1.00 46.15
ATOM 206 O GLU 27 16.583 22.165 -8.818 1.00 45.46
ATOM 207 N ALA 28 16.979 23.448 -7.010 1.00 45.36
ATOM 208 CA ALA 28 17.821 24.404 -7.712 1.00 45.38
ATOM 209 CB ALA 28 18.254 25.493 -6.762 1.00 46.93
ATOM 210 C ALA 28 19.040 23.804 -8.395 1.00 45.46
ATOM 211 O ALA 28 19.693 22.904 -7.868 1.00 45.96
ATOM 212 N PRO 29 19.361 24.314 -9.593 1.00 43.34
ATOM 213 CD PRO 29 18.585 25.318 -10.336 1.00 43.59
ATOM 214 CA PRO 29 20.499 23.860 -10.392 1.00 39.84
ATOM 215 CB PRO 29 20.199 24.422 -11.789 1.00 40.87
ATOM 216 CG PRO 29 18.731 24.809 -11.738 1.00 43.29
ATOM 217 C PRO 29 21.768 24.456 -9.814 1.00 36.35
ATOM 218 O PRO 29 21.745 25.532 -9.218 1.00 32.62
ATOM 219 N ALA 30 22.879 23.760 -9.981 1.00 34.74
ATOM 220 CA ALA 30 24.118 24.291 -9.462 1.00 40.14
ATOM 221 CB ALA 30 25.293 23.443 -9.913 1.00 39.49
ATOM 222 C ALA 30 24.224 25.704 -10.015 1.00 43.07
ATOM 223 O ALA 30 23.557 26.041 -10.999 1.00 45.14
ATOM 224 N GLY 31 25.039 26.532 -9.366 1.00 43.67
ATOM 225 CA GLY 31 25.209 27.903 -9.812 1.00 44.09
ATOM 226 C GLY 31 24.044 28.817 -9.486 1.00 45.12
ATOM 227 O GLY 31 24.216 30.036 -9.426 1.00 45.37
ATOM 228 N ALA 32 22.861 28.236 -9.275 1.00 45.29
ATOM 229 CA ALA 32 21.663 29.012 -8.942 1.00 44.96
ATOM 230 CB ALA 32 20.427 28.091 -8.837 1.00 46.26
ATOM 231 C ALA 32 21.874 29.754 -7.626 1.00 44.27
ATOM 232 O ALA 32 22.799 29.449 -6.871 1.00 45.48
ATOM 233 N LYS 33 21.014 30.731 -7.362 1.00 44.05
ATOM 234 CA LYS 33 21.096 31.530 -6.147 1.00 43.46
ATOM 235 CB LYS 33 20.903 33.007 -6.469 1.00 45.85
ATOM 236 CG LYS 33 21.975 33.588 -7.362 1.00 46.87
ATOM 237 CD LYS 33 23.287 33.640 -6.609 1.00 48.03
ATOM 238 CE LYS 33 24.349 34.400 -7.378 1.00 48.31
ATOM 239 NZ LYS 33 25.524 34.723 -6.507 1.00 47.98
ATOM 240 C LYS 33 20.041 31.120 -5.157 1.00 42.61
ATOM 241 O LYS 33 18.853 31.089 -5.472 1.00 44.51
ATOM 242 N VAL 34 20.480 30.820 -3.947 1.00 41.96
ATOM 243 CA VAL 34 19.559 30.423 -2.906 1.00 40.22
ATOM 244 CB VAL 34 20.314 30.132 -1.607 1.00 39.03
ATOM 245 CG1 VAL 34 19.333 29.774 -0.516 1.00 39.34
ATOM 246 CG2 VAL 34 21.301 28.982 -1.835 1.00 37.52
ATOM 247 C VAL 34 18.553 31.554 -2.731 1.00 41.33
ATOM 248 O VAL 34 18.917 32.711 -2.492 1.00 41.11
ATOM 249 N THR 35 17.284 31.206 -2.879 1.00 43.13
ATOM 250 CA THR 35 16.242 32.187 -2.801 1.00 46.82
ATOM 251 CB THR 35 15.904 32.677 -4.215 1.00 48.83
ATOM 252 OG1 THR 35 15.010 33.792 -4.143 1.00 55.17
ATOM 253 CG2 THR 35 15.253 31.559 -4.996 1.00 48.30
ATOM 254 C THR 35 14.987 31.629 -2.153 1.00 47.64
ATOM 255 O THR 35 14.723 30.432 -2.197 1.00 50.05
ATOM 256 N CYS 36 14.209 32.525 -1.561 1.00 47.99
ATOM 257 CA CYS 36 12.974 32.160 -0.895 1.00 47.27
ATOM 258 C CYS 36 11.993 33.270 -1.071 1.00 49.06
ATOM 259 O CYS 36 12.351 34.443 -1.147 1.00 51.86
ATOM 260 CB CYS 36 13.206 31.961 0.599 1.00 47.51
ATOM 261 SG CYS 36 14.721 31.004 0.946 1.00 46.80
ATOM 262 N SER 37 10.738 32.873 -1.122 1.00 50.29
ATOM 263 CA SER 37 9.657 33.811 -1.253 1.00 51.64
ATOM 264 CB SER 37 8.606 33.282 -2.224 1.00 51.71
ATOM 265 OG SER 37 7.920 32.176 -1.671 1.00 51.99
ATOM 266 C SER 37 9.047 33.954 0.132 1.00 52.79
ATOM 267 O SER 37 9.227 33.081 1.009 1.00 54.99
ATOM 268 N GLY 38 8.313 35.049 0.319 1.00 52.11
ATOM 269 CA GLY 38 7.683 35.300 1.601 1.00 47.78
ATOM 270 C GLY 38 8.741 35.668 2.616 1.00 46.33
ATOM 271 O GLY 38 8.597 35.336 3.799 1.00 41.87
ATOM 272 N ASN 39 9.794 36.347 2.142 1.00 46.02
ATOM 273 CA ASN 39 10.905 36.774 2.986 1.00 43.85
ATOM 274 CB ASN 39 10.435 37.873 3.941 1.00 47.51
ATOM 275 CG ASN 39 11.282 39.114 3.842 1.00 54.53
ATOM 276 OD1 ASN 39 12.286 39.258 4.551 1.00 56.71
ATOM 277 ND2 ASN 39 10.902 40.017 2.938 1.00 55.69
ATOM 278 C ASN 39 11.422 35.574 3.762 1.00 41.94
ATOM 279 O ASN 39 11.950 35.703 4.881 1.00 38.41
ATOM 280 N GLY 40 11.274 34.406 3.143 1.00 41.26
ATOM 281 CA GLY 40 11.689 33.183 3.785 1.00 39.14
ATOM 282 C GLY 40 13.131 33.124 4.234 1.00 37.57
ATOM 283 O GLY 40 13.427 32.461 5.228 1.00 38.05
ATOM 284 N CYS 41 14.025 33.809 3.521 1.00 37.14
ATOM 285 CA CYS 41 15.449 33.756 3.851 1.00 36.29
ATOM 286 C CYS 41 16.257 34.924 3.300 1.00 37.23
ATOM 287 O CYS 41 17.256 34.731 2.617 1.00 36.42
ATOM 288 CB CYS 41 16.028 32.456 3.305 1.00 33.97
ATOM 289 SG CYS 41 16.100 32.411 1.482 1.00 36.33
ATOM 290 N PRO 42 15.850 36.153 3.621 1.00 38.20
ATOM 291 CD PRO 42 14.779 36.487 4.576 1.00 39.84
ATOM 292 CA PRO 42 16.540 37.356 3.148 1.00 38.13
ATOM 293 CB PRO 42 15.715 38.485 3.766 1.00 37.35
ATOM 294 CG PRO 42 15.177 37.862 5.027 1.00 37.04
ATOM 295 C PRO 42 18.047 37.480 3.424 1.00 38.39
ATOM 296 O PRO 42 18.790 37.903 2.548 1.00 38.45
ATOM 297 N THR 43 18.504 37.126 4.622 1.00 38.67
ATOM 298 CA THR 43 19.931 37.235 4.932 1.00 38.85
ATOM 299 CB THR 43 20.251 36.818 6.395 1.00 36.92
ATOM 300 OG1 THR 43 19.613 37.727 7.299 1.00 37.95
ATOM 301 CG2 THR 43 21.753 36.853 6.653 1.00 33.96
ATOM 302 C THR 43 20.686 36.334 3.993 1.00 38.46
ATOM 303 O THR 43 21.789 36.636 3.556 1.00 39.61
ATOM 304 N VAL 44 20.081 35.209 3.684 1.00 38.02
ATOM 305 CA VAL 44 20.730 34.302 2.792 1.00 39.52
ATOM 306 CB VAL 44 19.982 32.988 2.750 1.00 36.82
ATOM 307 CG1 VAL 44 20.601 32.105 1.712 1.00 37.04
ATOM 308 CG2 VAL 44 20.028 32.321 4.106 1.00 36.16
ATOM 309 C VAL 44 20.794 34.949 1.412 1.00 43.60
ATOM 310 O VAL 44 21.843 34.950 0.771 1.00 41.94
ATOM 311 N GLN 45 19.682 35.516 0.957 1.00 47.86
ATOM 312 CA GLN 45 19.684 36.166 -0.347 1.00 50.53
ATOM 313 CB GLN 45 18.267 36.548 -0.780 1.00 46.99
ATOM 314 CG GLN 45 17.327 35.370 -0.912 1.00 49.29
ATOM 315 CD GLN 45 15.924 35.790 -1.305 1.00 50.89
ATOM 316 OE1 GLN 45 15.396 36.774 -0.795 1.00 54.31
ATOM 317 NE2 GLN 45 15.311 35.044 -2.205 1.00 47.74
ATOM 318 C GLN 45 20.568 37.405 -0.289 1.00 52.82
ATOM 319 O GLN 45 21.341 37.667 -1.207 1.00 54.98
ATOM 320 N SER 46 20.462 38.164 0.795 1.00 54.53
ATOM 321 CA SER 46 21.278 39.360 0.946 1.00 54.05
ATOM 322 CB SER 46 21.162 39.907 2.356 1.00 52.75
ATOM 323 OG SER 46 22.332 40.647 2.655 1.00 52.40
ATOM 324 C SER 46 22.737 39.045 0.667 1.00 53.72
ATOM 325 O SER 46 23.455 39.812 0.033 1.00 52.04
ATOM 326 N ASN 47 23.170 37.907 1.180 1.00 54.08
ATOM 327 CA ASN 47 24.524 37.462 0.975 1.00 54.09
ATOM 328 CB ASN 47 24.926 36.519 2.094 1.00 52.99
ATOM 329 CG ASN 47 24.669 37.109 3.446 1.00 55.12
ATOM 330 OD1 ASN 47 24.826 36.447 4.464 1.00 55.92
ATOM 331 ND2 ASN 47 24.267 38.376 3.470 1.00 56.97
ATOM 332 C ASN 47 24.483 36.722 -0.335 1.00 55.62
ATOM 333 O ASN 47 23.409 36.481 -0.895 1.00 55.92
ATOM 334 N GLY 48 25.651 36.338 -0.815 1.00 55.14
ATOM 335 CA GLY 48 25.706 35.629 -2.071 1.00 53.83
ATOM 336 C GLY 48 25.619 34.127 -1.991 1.00 51.96
ATOM 337 O GLY 48 26.424 33.435 -2.596 1.00 54.44
ATOM 338 N ALA 49 24.632 33.615 -1.269 1.00 49.83
ATOM 339 CA ALA 49 24.465 32.166 -1.130 1.00 47.17
ATOM 340 CB ALA 49 23.393 31.863 -0.106 1.00 44.77
ATOM 341 C ALA 49 24.108 31.506 -2.458 1.00 45.91
ATOM 342 O ALA 49 23.130 31.879 -3.108 1.00 44.92
ATOM 343 N THR 50 24.895 30.513 -2.855 1.00 44.61
ATOM 344 CA THR 50 24.658 29.827 -4.119 1.00 45.57
ATOM 345 CB THR 50 25.711 30.252 -5.137 1.00 44.64
ATOM 346 OG1 THR 50 27.016 30.057 -4.572 1.00 48.93
ATOM 347 CG2 THR 50 25.543 31.714 -5.484 1.00 44.18
ATOM 348 C THR 50 24.689 28.303 -3.973 1.00 44.90
ATOM 349 O THR 50 25.323 27.774 -3.058 1.00 47.41
ATOM 350 N ILE 51 24.001 27.606 -4.878 1.00 41.51
ATOM 351 CA ILE 51 23.939 26.147 -4.854 1.00 40.26
ATOM 352 CB ILE 51 22.705 25.608 -5.608 1.00 40.04
ATOM 353 CG2 ILE 51 22.656 24.083 -5.524 1.00 40.44
ATOM 354 CG1 ILE 51 21.431 26.191 -5.012 1.00 44.06
ATOM 355 CD1 ILE 51 20.980 27.438 -5.720 1.00 43.76
ATOM 356 C ILE 51 25.155 25.502 -5.491 1.00 39.87
ATOM 357 O ILE 51 25.649 25.955 -6.523 1.00 42.31
ATOM 358 N VAL 52 25.631 24.429 -4.877 1.00 38.22
ATOM 359 CA VAL 52 26.763 23.712 -5.415 1.00 35.17
ATOM 360 CB VAL 52 27.711 23.308 -4.299 1.00 33.49
ATOM 361 CG1 VAL 52 28.920 22.570 -4.869 1.00 34.02
ATOM 362 CG2 VAL 52 28.152 24.553 -3.549 1.00 29.71
ATOM 363 C VAL 52 26.144 22.485 -6.076 1.00 35.33
ATOM 364 O VAL 52 26.728 21.887 -7.001 1.00 36.26
ATOM 365 N ALA 53 24.936 22.144 -5.619 1.00 35.25
ATOM 366 CA ALA 53 24.194 21.003 -6.152 1.00 35.42
ATOM 367 CB ALA 53 25.073 19.748 -6.117 1.00 31.66
ATOM 368 C ALA 53 22.951 20.793 -5.293 1.00 33.55
ATOM 369 O ALA 53 22.942 21.153 -4.115 1.00 35.15
ATOM 370 N SER 54 21.890 20.247 -5.874 1.00 32.33
ATOM 371 CA SER 54 20.688 19.973 -5.084 1.00 31.79
ATOM 372 CB SER 54 19.502 20.832 -5.524 1.00 30.57
ATOM 373 OG SER 54 19.105 20.503 -6.840 1.00 37.05
ATOM 374 C SER 54 20.383 18.505 -5.309 1.00 31.23
ATOM 375 O SER 54 20.939 17.883 -6.230 1.00 27.55
ATOM 376 N PHE 55 19.501 17.939 -4.500 1.00 32.84
ATOM 377 CA PHE 55 19.243 16.526 -4.654 1.00 35.43
ATOM 378 CB PHE 55 20.399 15.741 -4.053 1.00 35.14
ATOM 379 CG PHE 55 20.922 16.322 -2.761 1.00 32.14
ATOM 380 CD1 PHE 55 22.222 16.856 -2.704 1.00 29.42
ATOM 381 CD2 PHE 55 20.155 16.305 -1.599 1.00 30.22
ATOM 382 CE1 PHE 55 22.745 17.352 -1.520 1.00 25.73
ATOM 383 CE2 PHE 55 20.693 16.810 -0.398 1.00 31.85
ATOM 384 CZ PHE 55 21.978 17.325 -0.368 1.00 29.09
ATOM 385 C PHE 55 17.979 16.116 -3.975 1.00 35.04
ATOM 386 O PHE 55 17.518 16.777 -3.037 1.00 39.64
ATOM 387 N THR 56 17.429 15.000 -4.436 1.00 29.99
ATOM 388 CA THR 56 16.191 14.488 -3.864 1.00 28.55
ATOM 389 CB THR 56 14.945 15.096 -4.590 1.00 27.79
ATOM 390 OG1 THR 56 14.733 14.425 -5.844 1.00 23.97
ATOM 391 CG2 THR 56 15.178 16.600 -4.870 1.00 21.19
ATOM 392 C THR 56 16.129 12.958 -3.945 1.00 27.76
ATOM 393 O THR 56 16.540 12.352 -4.945 1.00 27.35
ATOM 394 N GLY 57 15.659 12.336 -2.869 1.00 27.18
ATOM 395 CA GLY 57 15.530 10.890 -2.856 1.00 28.45
ATOM 396 C GLY 57 14.097 10.554 -3.204 1.00 29.19
ATOM 397 O GLY 57 13.209 10.576 -2.352 1.00 28.30
ATOM 398 N SER 58 13.874 10.243 -4.475 1.00 31.31
ATOM 399 CA SER 58 12.547 9.912 -4.978 1.00 34.45
ATOM 400 CB SER 58 12.654 9.347 -6.397 1.00 38.20
ATOM 401 OG SER 58 11.381 9.224 -7.010 1.00 44.69
ATOM 402 C SER 58 11.858 8.901 -4.076 1.00 33.61
ATOM 403 O SER 58 10.735 9.126 -3.620 1.00 34.55
ATOM 404 N LYS 59 12.535 7.796 -3.810 1.00 34.29
ATOM 405 CA LYS 59 11.974 6.761 -2.968 1.00 34.68
ATOM 406 CB LYS 59 12.943 5.587 -2.921 1.00 36.22
ATOM 407 CG LYS 59 12.562 4.459 -1.966 1.00 38.49
ATOM 408 CD LYS 59 13.605 3.338 -2.000 1.00 48.21
ATOM 409 CE LYS 59 13.141 2.110 -1.216 1.00 54.13
ATOM 410 NZ LYS 59 14.126 0.972 -1.246 1.00 54.56
ATOM 411 C LYS 59 11.644 7.199 -1.549 1.00 33.98
ATOM 412 O LYS 59 10.735 6.654 -0.921 1.00 39.25
ATOM 413 N THR 60 12.352 8.193 -1.033 1.00 32.34
ATOM 414 CA THR 60 12.114 8.569 0.347 1.00 29.15
ATOM 415 CB THR 60 13.426 8.369 1.109 1.00 27.10
ATOM 416 OG1 THR 60 13.175 7.767 2.378 1.00 23.98
ATOM 417 CG2 THR 60 14.143 9.696 1.268 1.00 29.07
ATOM 418 C THR 60 11.520 9.963 0.636 1.00 30.25
ATOM 419 O THR 60 11.184 10.270 1.779 1.00 28.21
ATOM 420 N GLY 61 11.381 10.804 -0.384 1.00 30.45
ATOM 421 CA GLY 61 10.815 12.119 -0.148 1.00 29.54
ATOM 422 C GLY 61 11.822 13.108 0.398 1.00 31.39
ATOM 423 O GLY 61 11.553 14.299 0.458 1.00 34.11
ATOM 424 N ILE 62 12.989 12.640 0.808 1.00 29.86
ATOM 425 CA ILE 62 13.961 13.583 1.294 1.00 28.85
ATOM 426 CB ILE 62 15.203 12.887 1.857 1.00 29.86
ATOM 427 CG2 ILE 62 15.952 12.192 0.729 1.00 30.75
ATOM 428 CG1 ILE 62 16.091 13.922 2.572 1.00 30.47
ATOM 429 CD1 ILE 62 15.475 14.557 3.844 1.00 24.98
ATOM 430 C ILE 62 14.373 14.495 0.140 1.00 30.26
ATOM 431 O ILE 62 14.013 14.278 -1.018 1.00 30.97
ATOM 432 N GLY 63 15.130 15.526 0.481 1.00 29.70
ATOM 433 CA GLY 63 15.599 16.488 -0.492 1.00 29.18
ATOM 434 C GLY 63 16.466 17.450 0.281 1.00 30.92
ATOM 435 O GLY 63 16.366 17.524 1.516 1.00 32.28
ATOM 436 N GLY 64 17.309 18.186 -0.437 1.00 30.23
ATOM 437 CA GLY 64 18.192 19.141 0.201 1.00 31.31
ATOM 438 C GLY 64 19.222 19.699 -0.759 1.00 32.75
ATOM 439 O GLY 64 19.257 19.312 -1.937 1.00 35.66
ATOM 440 N TYR 65 20.084 20.579 -0.251 1.00 31.95
ATOM 441 CA TYR 65 21.113 21.206 -1.075 1.00 32.66
ATOM 442 CB TYR 65 20.546 22.488 -1.709 1.00 34.11
ATOM 443 CG TYR 65 20.241 23.601 -0.703 1.00 30.58
ATOM 444 CD1 TYR 65 21.260 24.429 -0.204 1.00 29.89
ATOM 445 CE1 TYR 65 20.991 25.404 0.765 1.00 31.16
ATOM 446 CD2 TYR 65 18.946 23.791 -0.203 1.00 31.70
ATOM 447 CE2 TYR 65 18.682 24.762 0.767 1.00 31.85
ATOM 448 CZ TYR 65 19.704 25.567 1.251 1.00 33.02
ATOM 449 OH TYR 65 19.436 26.519 2.234 1.00 30.17
ATOM 450 C TYR 65 22.384 21.544 -0.295 1.00 31.93
ATOM 451 O TYR 65 22.420 21.458 0.946 1.00 33.75
ATOM 452 N VAL 66 23.425 21.898 -1.050 1.00 31.26
ATOM 453 CA VAL 66 24.730 22.308 -0.519 1.00 29.99
ATOM 454 CB VAL 66 25.879 21.384 -0.995 1.00 25.14
ATOM 455 CG1 VAL 66 27.229 21.928 -0.486 1.00 19.75
ATOM 456 CG2 VAL 66 25.653 19.983 -0.517 1.00 19.11
ATOM 457 C VAL 66 24.977 23.683 -1.138 1.00 32.47
ATOM 458 O VAL 66 24.944 23.819 -2.378 1.00 34.11
ATOM 459 N ALA 67 25.229 24.688 -0.297 1.00 34.55
ATOM 460 CA ALA 67 25.456 26.034 -0.794 1.00 36.86
ATOM 461 CB ALA 67 24.248 26.883 -0.501 1.00 37.43
ATOM 462 C ALA 67 26.691 26.686 -0.205 1.00 38.04
ATOM 463 O ALA 67 27.185 26.266 0.847 1.00 37.89
ATOM 464 N THR 68 27.142 27.746 -0.877 1.00 38.87
ATOM 465 CA THR 68 28.320 28.515 -0.477 1.00 41.49
ATOM 466 CB THR 68 29.374 28.482 -1.555 1.00 40.24
ATOM 467 OG1 THR 68 28.781 28.909 -2.786 1.00 39.38
ATOM 468 CG2 THR 68 29.916 27.078 -1.695 1.00 37.17
ATOM 469 C THR 68 27.974 29.971 -0.209 1.00 44.07
ATOM 470 O THR 68 27.303 30.619 -1.007 1.00 45.44
ATOM 471 N ASP 69 28.481 30.476 0.914 1.00 46.41
ATOM 472 CA ASP 69 28.240 31.844 1.379 1.00 48.43
ATOM 473 CB ASP 69 27.760 31.808 2.834 1.00 52.61
ATOM 474 CG ASP 69 26.780 32.927 3.167 1.00 56.38
ATOM 475 OD1 ASP 69 27.148 34.118 3.048 1.00 56.17
ATOM 476 OD2 ASP 69 25.634 32.602 3.556 1.00 58.58
ATOM 477 C ASP 69 29.493 32.716 1.294 1.00 48.33
ATOM 478 O ASP 69 30.354 32.686 2.182 1.00 49.35
ATOM 479 N PRO 70 29.611 33.507 0.221 1.00 46.85
ATOM 480 CD PRO 70 28.598 33.759 -0.816 1.00 45.47
ATOM 481 CA PRO 70 30.773 34.384 0.052 1.00 47.08
ATOM 482 CB PRO 70 30.425 35.181 -1.210 1.00 46.69
ATOM 483 CG PRO 70 28.929 35.155 -1.241 1.00 45.84
ATOM 484 C PRO 70 30.991 35.281 1.276 1.00 47.53
ATOM 485 O PRO 70 32.110 35.455 1.749 1.00 47.58
ATOM 486 N THR 71 29.895 35.833 1.782 1.00 46.80
ATOM 487 CA THR 71 29.901 36.723 2.938 1.00 45.48
ATOM 488 CB THR 71 28.501 37.315 3.177 1.00 43.99
ATOM 489 OG1 THR 71 28.104 38.086 2.041 1.00 40.66
ATOM 490 CG2 THR 71 28.497 38.181 4.408 1.00 43.58
ATOM 491 C THR 71 30.312 36.029 4.231 1.00 45.79
ATOM 492 O THR 71 31.178 36.506 4.960 1.00 45.02
ATOM 493 N ARG 72 29.671 34.904 4.521 1.00 46.99
ATOM 494 CA ARG 72 29.954 34.171 5.746 1.00 47.70
ATOM 495 CB ARG 72 28.724 33.384 6.159 1.00 48.08
ATOM 496 CG ARG 72 27.681 34.206 6.859 1.00 48.15
ATOM 497 CD ARG 72 26.521 33.312 7.196 1.00 46.29
ATOM 498 NE ARG 72 25.995 33.592 8.524 1.00 46.49
ATOM 499 CZ ARG 72 26.452 33.057 9.651 1.00 44.95
ATOM 500 NH1 ARG 72 27.457 32.193 9.625 1.00 50.14
ATOM 501 NH2 ARG 72 25.905 33.400 10.809 1.00 41.99
ATOM 502 C ARG 72 31.143 33.217 5.681 1.00 46.99
ATOM 503 O ARG 72 31.549 32.655 6.709 1.00 44.85
ATOM 504 N LYS 73 31.698 33.035 4.484 1.00 46.91
ATOM 505 CA LYS 73 32.822 32.128 4.308 1.00 46.89
ATOM 506 CB LYS 73 34.098 32.703 4.929 1.00 51.74
ATOM 507 CG LYS 73 34.809 33.782 4.112 1.00 57.40
ATOM 508 CD LYS 73 36.162 34.081 4.783 1.00 63.05
ATOM 509 CE LYS 73 37.203 34.577 3.777 1.00 67.22
ATOM 510 NZ LYS 73 38.607 34.596 4.319 1.00 69.40
ATOM 511 C LYS 73 32.516 30.792 4.968 1.00 44.83
ATOM 512 O LYS 73 33.140 30.419 5.969 1.00 42.64
ATOM 513 N GLU 74 31.544 30.081 4.409 1.00 42.80
ATOM 514 CA GLU 74 31.164 28.781 4.926 1.00 41.44
ATOM 515 CB GLU 74 30.311 28.923 6.191 1.00 41.39
ATOM 516 CG GLU 74 28.989 29.654 5.992 1.00 40.80
ATOM 517 CD GLU 74 28.215 29.819 7.295 1.00 41.18
ATOM 518 OE1 GLU 74 26.988 30.036 7.239 1.00 37.73
ATOM 519 OE2 GLU 74 28.831 29.738 8.376 1.00 41.62
ATOM 520 C GLU 74 30.393 27.999 3.876 1.00 39.97
ATOM 521 O GLU 74 30.014 28.534 2.825 1.00 39.67
ATOM 522 N ILE 75 30.181 26.723 4.179 1.00 37.40
ATOM 523 CA ILE 75 29.464 25.807 3.309 1.00 33.79
ATOM 524 CB ILE 75 30.418 24.715 2.753 1.00 33.51
ATOM 525 CG2 ILE 75 29.679 23.821 1.766 1.00 33.40
ATOM 526 CG1 ILE 75 31.626 25.362 2.062 1.00 34.17
ATOM 527 CD1 ILE 75 32.723 24.362 1.645 1.00 32.76
ATOM 528 C ILE 75 28.394 25.135 4.157 1.00 31.88
ATOM 529 O ILE 75 28.711 24.432 5.127 1.00 30.16
ATOM 530 N VAL 76 27.130 25.354 3.806 1.00 29.47
ATOM 531 CA VAL 76 26.043 24.736 4.571 1.00 30.29
ATOM 532 CB VAL 76 24.930 25.758 4.986 1.00 29.82
ATOM 533 CG1 VAL 76 25.539 27.145 5.281 1.00 25.34
ATOM 534 CG2 VAL 76 23.870 25.815 3.913 1.00 29.32
ATOM 535 C VAL 76 25.363 23.627 3.767 1.00 29.05
ATOM 536 O VAL 76 25.384 23.655 2.523 1.00 32.60
ATOM 537 N VAL 77 24.757 22.668 4.484 1.00 29.82
ATOM 538 CA VAL 77 24.037 21.517 3.879 1.00 29.11
ATOM 539 CB VAL 77 24.688 20.162 4.261 1.00 27.37
ATOM 540 CG1 VAL 77 24.313 19.102 3.250 1.00 24.87
ATOM 541 CG2 VAL 77 26.179 20.302 4.359 1.00 25.61
ATOM 542 C VAL 77 22.619 21.486 4.429 1.00 28.13
ATOM 543 O VAL 77 22.412 21.080 5.576 1.00 29.04
ATOM 544 N SER 78 21.649 21.894 3.618 1.00 27.68
ATOM 545 CA SER 78 20.267 21.957 4.083 1.00 29.69
ATOM 546 CB SER 78 19.669 23.313 3.737 1.00 29.26
ATOM 547 OG SER 78 20.187 24.292 4.582 1.00 35.26
ATOM 548 C SER 78 19.333 20.870 3.580 1.00 28.37
ATOM 549 O SER 78 19.176 20.652 2.375 1.00 32.05
ATOM 550 N PHE 79 18.686 20.195 4.509 1.00 26.09
ATOM 551 CA PHE 79 17.774 19.148 4.120 1.00 27.58
ATOM 552 CB PHE 79 18.086 17.899 4.894 1.00 27.89
ATOM 553 CG PHE 79 19.512 17.483 4.792 1.00 23.20
ATOM 554 CD1 PHE 79 20.499 18.090 5.561 1.00 23.22
ATOM 555 CD2 PHE 79 19.868 16.445 3.954 1.00 22.33
ATOM 556 CE1 PHE 79 21.813 17.649 5.495 1.00 23.58
ATOM 557 CE2 PHE 79 21.172 16.006 3.886 1.00 21.16
ATOM 558 CZ PHE 79 22.143 16.605 4.659 1.00 22.90
ATOM 559 C PHE 79 16.411 19.660 4.471 1.00 30.97
ATOM 560 O PHE 79 16.260 20.394 5.467 1.00 31.30
ATOM 561 N ARG 80 15.419 19.272 3.675 1.00 34.09
ATOM 562 CA ARG 80 14.069 19.757 3.884 1.00 37.31
ATOM 563 CB ARG 80 13.423 20.015 2.541 1.00 39.96
ATOM 564 CG ARG 80 13.054 18.739 1.827 1.00 44.84
ATOM 565 CD ARG 80 12.388 19.015 0.509 1.00 43.90
ATOM 566 NE ARG 80 11.986 17.775 -0.140 1.00 44.13
ATOM 567 CZ ARG 80 11.652 17.692 -1.421 1.00 47.81
ATOM 568 NH1 ARG 80 11.673 18.779 -2.185 1.00 47.38
ATOM 569 NH2 ARG 80 11.308 16.522 -1.943 1.00 50.47
ATOM 570 C ARG 80 13.176 18.819 4.674 1.00 38.95
ATOM 571 O ARG 80 13.176 17.603 4.441 1.00 38.69
ATOM 572 N GLY 81 12.381 19.397 5.573 1.00 40.20
ATOM 573 CA GLY 81 11.482 18.604 6.393 1.00 42.83
ATOM 574 C GLY 81 10.232 18.077 5.687 1.00 45.60
ATOM 575 O GLY 81 10.196 17.974 4.443 1.00 46.04
ATOM 576 N SER 82 9.207 17.739 6.475 1.00 48.26
ATOM 577 CA SER 82 7.961 17.210 5.937 1.00 51.78
ATOM 578 CB SER 82 7.335 16.244 6.947 1.00 53.73
ATOM 579 OG SER 82 6.254 15.513 6.393 1.00 58.63
ATOM 580 C SER 82 6.998 18.348 5.632 1.00 53.53
ATOM 581 O SER 82 6.751 19.219 6.471 1.00 54.68
ATOM 582 N ILE 83 6.464 18.339 4.420 1.00 52.86
ATOM 583 CA ILE 83 5.522 19.355 4.003 1.00 52.55
ATOM 584 CB ILE 83 5.488 19.443 2.479 1.00 53.58
ATOM 585 CG2 ILE 83 6.841 19.896 1.979 1.00 53.86
ATOM 586 CG1 ILE 83 5.133 18.077 1.885 1.00 53.41
ATOM 587 CD1 ILE 83 5.129 18.011 0.381 1.00 53.83
ATOM 588 C ILE 83 4.157 18.946 4.527 1.00 52.26
ATOM 589 O ILE 83 3.168 19.677 4.375 1.00 51.93
ATOM 590 N ASN 84 4.122 17.770 5.153 1.00 48.94
ATOM 591 CA ASN 84 2.888 17.229 5.711 1.00 46.21
ATOM 592 CB ASN 84 2.353 16.135 4.805 1.00 46.90
ATOM 593 CG ASN 84 0.855 15.998 4.902 1.00 49.97
ATOM 594 OD1 ASN 84 0.271 16.163 5.985 1.00 51.10
ATOM 595 ND2 ASN 84 0.213 15.695 3.765 1.00 46.93
ATOM 596 C ASN 84 3.133 16.649 7.097 1.00 44.37
ATOM 597 O ASN 84 2.867 15.489 7.354 1.00 44.26
ATOM 598 N ILE 85 3.629 17.473 8.001 1.00 38.99
ATOM 599 CA ILE 85 3.945 17.006 9.341 1.00 33.20
ATOM 600 CB ILE 85 4.619 18.087 10.145 1.00 28.90
ATOM 601 CG2 ILE 85 3.577 19.113 10.608 1.00 27.23
ATOM 602 CG1 ILE 85 5.455 17.418 11.242 1.00 25.20
ATOM 603 CD1 ILE 85 6.778 16.808 10.681 1.00 20.62
ATOM 604 C ILE 85 2.826 16.453 10.207 1.00 32.57
ATOM 605 O ILE 85 3.035 15.504 10.955 1.00 32.52
ATOM 606 N ARG 86 1.641 17.037 10.143 1.00 35.06
ATOM 607 CA ARG 86 0.577 16.497 10.963 1.00 38.21
ATOM 608 CB ARG 86 -0.723 17.259 10.755 1.00 42.23
ATOM 609 CG ARG 86 -1.836 16.354 10.257 1.00 51.70
ATOM 610 CD ARG 86 -3.214 16.998 10.318 1.00 54.87
ATOM 611 NE ARG 86 -4.172 16.168 11.047 1.00 53.16
ATOM 612 CZ ARG 86 -4.345 16.211 12.362 1.00 54.60
ATOM 613 NH1 ARG 86 -3.623 17.049 13.092 1.00 54.61
ATOM 614 NH2 ARG 86 -5.237 15.419 12.944 1.00 52.09
ATOM 615 C ARG 86 0.410 15.056 10.502 1.00 38.35
ATOM 616 O ARG 86 0.181 14.158 11.289 1.00 37.91
ATOM 617 N ASN 87 0.543 14.844 9.205 1.00 39.43
ATOM 618 CA ASN 87 0.421 13.510 8.652 1.00 41.29
ATOM 619 CB ASN 87 0.679 13.544 7.145 1.00 45.33
ATOM 620 CG ASN 87 0.464 12.198 6.486 1.00 48.42
ATOM 621 OD1 ASN 87 0.764 12.021 5.305 1.00 48.98
ATOM 622 ND2 ASN 87 -0.065 11.239 7.245 1.00 50.03
ATOM 623 C ASN 87 1.425 12.569 9.312 1.00 39.38
ATOM 624 O ASN 87 1.049 11.532 9.870 1.00 40.50
ATOM 625 N TRP 88 2.701 12.951 9.236 1.00 38.35
ATOM 626 CA TRP 88 3.797 12.170 9.792 1.00 33.00
ATOM 627 CB TRP 88 5.113 12.961 9.750 1.00 28.10
ATOM 628 CG TRP 88 6.309 12.112 10.132 1.00 28.88
ATOM 629 CD2 TRP 88 6.975 12.066 11.400 1.00 23.66
ATOM 630 CE2 TRP 88 7.932 11.005 11.333 1.00 24.60
ATOM 631 CE3 TRP 88 6.859 12.808 12.589 1.00 24.28
ATOM 632 CD1 TRP 88 6.878 11.128 9.373 1.00 28.94
ATOM 633 NE1 TRP 88 7.850 10.452 10.085 1.00 22.39
ATOM 634 CZ2 TRP 88 8.765 10.672 12.416 1.00 23.76
ATOM 635 CZ3 TRP 88 7.697 12.478 13.675 1.00 25.97
ATOM 636 CH2 TRP 88 8.636 11.413 13.572 1.00 24.75
ATOM 637 C TRP 88 3.509 11.800 11.228 1.00 33.22
ATOM 638 O TRP 88 3.827 10.697 11.681 1.00 34.17
ATOM 639 N LEU 89 2.919 12.731 11.957 1.00 33.69
ATOM 640 CA LEU 89 2.629 12.450 13.334 1.00 35.69
ATOM 641 CB LEU 89 2.199 13.726 14.056 1.00 34.51
ATOM 642 CG LEU 89 3.267 14.770 14.425 1.00 31.52
ATOM 643 CD1 LEU 89 2.615 15.814 15.303 1.00 31.01
ATOM 644 CD2 LEU 89 4.415 14.153 15.183 1.00 27.75
ATOM 645 C LEU 89 1.570 11.372 13.464 1.00 37.12
ATOM 646 O LEU 89 1.710 10.463 14.264 1.00 42.41
ATOM 647 N THR 90 0.513 11.444 12.676 1.00 35.52
ATOM 648 CA THR 90 -0.531 10.444 12.799 1.00 33.89
ATOM 649 CB THR 90 -1.687 10.772 11.895 1.00 32.79
ATOM 650 OG1 THR 90 -1.228 10.778 10.538 1.00 29.17
ATOM 651 CG2 THR 90 -2.254 12.129 12.274 1.00 31.57
ATOM 652 C THR 90 -0.053 9.039 12.484 1.00 34.21
ATOM 653 O THR 90 -0.640 8.065 12.958 1.00 34.70
ATOM 654 N ASN 91 1.009 8.933 11.694 1.00 33.44
ATOM 655 CA ASN 91 1.540 7.628 11.326 1.00 34.05
ATOM 656 CB ASN 91 2.364 7.747 10.067 1.00 34.95
ATOM 657 CG ASN 91 1.503 7.962 8.866 1.00 40.50
ATOM 658 OD1 ASN 91 1.972 8.382 7.816 1.00 44.74
ATOM 659 ND2 ASN 91 0.213 7.666 9.011 1.00 45.23
ATOM 660 C ASN 91 2.354 6.954 12.389 1.00 33.80
ATOM 661 O ASN 91 2.441 5.731 12.403 1.00 34.95
ATOM 662 N LEU 92 2.945 7.751 13.275 1.00 34.11
ATOM 663 CA LEU 92 3.774 7.242 14.370 1.00 34.02
ATOM 664 CB LEU 92 2.956 6.320 15.281 1.00 33.05
ATOM 665 CG LEU 92 1.686 7.011 15.761 1.00 31.66
ATOM 666 CD1 LEU 92 0.976 6.160 16.793 1.00 28.13
ATOM 667 CD2 LEU 92 2.073 8.343 16.363 1.00 34.00
ATOM 668 C LEU 92 5.005 6.488 13.878 1.00 35.56
ATOM 669 O LEU 92 5.248 5.342 14.288 1.00 34.71
ATOM 670 N ASP 93 5.774 7.139 13.014 1.00 36.76
ATOM 671 CA ASP 93 6.973 6.547 12.451 1.00 37.90
ATOM 672 CB ASP 93 7.515 7.500 11.393 1.00 38.96
ATOM 673 CG ASP 93 8.548 6.850 10.505 1.00 42.87
ATOM 674 OD1 ASP 93 9.435 6.144 11.044 1.00 43.52
ATOM 675 OD2 ASP 93 8.474 7.044 9.266 1.00 41.91
ATOM 676 C ASP 93 8.016 6.325 13.555 1.00 38.75
ATOM 677 O ASP 93 8.795 7.234 13.849 1.00 38.67
ATOM 678 N PHE 94 8.052 5.131 14.146 1.00 37.65
ATOM 679 CA PHE 94 9.001 4.886 15.217 1.00 36.97
ATOM 680 CB PHE 94 8.246 4.562 16.488 1.00 33.98
ATOM 681 CG PHE 94 7.409 5.684 16.988 1.00 36.52
ATOM 682 CD1 PHE 94 6.436 5.455 17.943 1.00 34.84
ATOM 683 CD2 PHE 94 7.621 6.981 16.537 1.00 37.51
ATOM 684 CE1 PHE 94 5.681 6.496 18.450 1.00 36.25
ATOM 685 CE2 PHE 94 6.874 8.046 17.032 1.00 39.25
ATOM 686 CZ PHE 94 5.894 7.800 18.002 1.00 39.66
ATOM 687 C PHE 94 10.042 3.804 14.992 1.00 35.93
ATOM 688 O PHE 94 11.111 3.828 15.619 1.00 34.66
ATOM 689 N ASP 95 9.755 2.861 14.110 1.00 33.05
ATOM 690 CA ASP 95 10.693 1.789 13.893 1.00 32.72
ATOM 691 CB ASP 95 10.241 1.010 12.705 1.00 33.93
ATOM 692 CG ASP 95 8.985 0.249 13.001 1.00 38.39
ATOM 693 OD1 ASP 95 8.254 0.678 13.919 1.00 36.80
ATOM 694 OD2 ASP 95 8.719 -0.770 12.331 1.00 38.25
ATOM 695 C ASP 95 12.137 2.191 13.759 1.00 33.61
ATOM 696 O ASP 95 12.480 3.217 13.188 1.00 32.88
ATOM 697 N GLN 96 13.004 1.368 14.311 1.00 32.68
ATOM 698 CA GLN 96 14.394 1.698 14.224 1.00 33.14
ATOM 699 CB GLN 96 14.902 2.281 15.537 1.00 31.37
ATOM 700 CG GLN 96 15.216 1.274 16.638 1.00 32.76
ATOM 701 CD GLN 96 15.964 1.910 17.834 1.00 33.37
ATOM 702 OE1 GLN 96 15.507 2.895 18.410 1.00 29.23
ATOM 703 NE2 GLN 96 17.108 1.338 18.203 1.00 32.04
ATOM 704 C GLN 96 15.293 0.574 13.808 1.00 35.47
ATOM 705 O GLN 96 15.246 -0.536 14.324 1.00 32.06
ATOM 706 N ASP 97 16.116 0.9041 2.830 1.00 37.28
ATOM 707 CA ASP 97 17.110 0.0021 2.296 1.00 39.67
ATOM 708 CB ASP 97 17.446 0.3711 0.858 1.00 39.54
ATOM 709 CG ASP 97 16.723 -0.484 9.871 1.00 42.24
ATOM 710 OD1 ASP 97 16.978 -0.344 8.656 1.00 43.67
ATOM 711 OD2 ASP 97 15.897 -1.305 10.318 1.00 48.30
ATOM 712 C ASP 97 18.369 0.129 13.121 1.00 39.69
ATOM 713 O ASP 97 18.495 1.016 13.964 1.00 41.13
ATOM 714 N GLU 98 19.305 -0.764 12.859 1.00 37.69
ATOM 715 CA GLU 98 20.550 -0.728 13.566 1.00 36.93
ATOM 716 CB GLU 98 21.158 -2.108 13.605 1.00 39.43
ATOM 717 CG GLU 98 20.107 -3.147 13.842 1.00 45.29
ATOM 718 CD GLU 98 20.688 -4.508 14.065 1.00 50.89
ATOM 719 OE1 GLU 98 19.909 -5.488 14.117 1.00 52.97
ATOM 720 OE2 GLU 98 21.925 -4.597 14.191 1.00 54.27
ATOM 721 C GLU 98 21.418 0.206 12.765 1.00 33.23
ATOM 722 O GLU 98 21.154 0.454 11.584 1.00 32.06
ATOM 723 N CYS 99 22.450 0.743 13.400 1.00 32.71
ATOM 724 CA CYS 99 23.351 1.665 12.722 1.00 34.26
ATOM 725 C CYS 99 24.782 1.212 13.008 1.00 34.77
ATOM 726 O CYS 99 25.023 0.504 13.991 1.00 34.87
ATOM 727 CB CYS 99 23.106 3.078 13.232 1.00 34.11
ATOM 728 SG CYS 99 24.557 3.884 13.952 1.00 42.37
ATOM 729 N SER 100 25.729 1.587 12.153 1.00 36.04
ATOM 730 CA SER 100 27.114 1.170 12.362 1.00 35.94
ATOM 731 CB SER 100 27.615 0.358 11.161 1.00 34.65
ATOM 732 OG SER 100 27.789 1.156 9.992 1.00 36.00
ATOM 733 C SER 100 28.042 2.355 12.599 1.00 38.47
ATOM 734 O SER 100 29.095 2.473 11.960 1.00 41.79
ATOM 735 N LEU 101 27.653 3.231 13.518 1.00 37.61
ATOM 736 CA LEU 101 28.451 4.405 13.822 1.00 36.68
ATOM 737 CB LEU 101 27.554 5.635 13.906 1.00 37.98
ATOM 738 CG LEU 101 27.063 6.258 12.595 1.00 37.48
ATOM 739 CD1 LEU 101 25.893 7.176 12.923 1.00 36.78
ATOM 740 CD2 LEU 101 28.187 7.031 11.888 1.00 37.90
ATOM 741 C LEU 101 29.163 4.187 15.145 1.00 34.88
ATOM 742 O LEU 101 30.266 4.728 15.380 1.00 32.30
ATOM 743 N THR 102 28.527 3.388 15.999 1.00 33.47
ATOM 744 CA THR 102 29.081 3.077 17.302 1.00 37.75
ATOM 745 CB THR 102 28.696 4.148 18.342 1.00 37.45
ATOM 746 OG1 THR 102 29.294 5.394 17.975 1.00 42.24
ATOM 747 CG2 THR 102 29.187 3.760 19.748 1.00 37.29
ATOM 748 C THR 102 28.526 1.738 17.737 1.00 39.39
ATOM 749 O THR 102 27.515 1.267 17.189 1.00 43.35
ATOM 750 N SER 103 29.196 1.127 18.708 1.00 39.79
ATOM 751 CA SER 103 28.766 -0.149 19.226 1.00 41.28
ATOM 752 CB SER 103 29.656 -0.555 20.386 1.00 42.67
ATOM 753 OG SER 103 29.204 -1.767 20.957 1.00 45.02
ATOM 754 C SER 103 27.326 -0.085 19.708 1.00 39.98
ATOM 755 O SER 103 26.935 0.866 20.384 1.00 41.43
ATOM 756 N GLY 104 26.544 -1.104 19.366 1.00 38.48
ATOM 757 CA GLY 104 25.156 -1.144 19.796 1.00 38.43
ATOM 758 C GLY 104 24.331 0.069 19.411 1.00 36.76
ATOM 759 O GLY 104 23.406 0.449 20.137 1.00 34.83
ATOM 760 N CYS 105 24.652 0.649 18.254 1.00 36.77
ATOM 761 CA CYS 105 23.970 1.838 17.733 1.00 35.81
ATOM 762 C CYS 105 22.632 1.565 17.036 1.00 34.27
ATOM 763 O CYS 105 22.562 0.759 16.086 1.00 35.72
ATOM 764 CB CYS 105 24.904 2.575 16.759 1.00 37.31
ATOM 765 SG CYS 105 24.192 4.053 15.956 1.00 41.61
ATOM 766 N GLY 106 21.580 2.241 17.513 1.00 32.57
ATOM 767 CA GLY 106 20.264 2.088 16.918 1.00 29.70
ATOM 768 C GLY 106 19.841 3.398 16.282 1.00 28.51
ATOM 769 O GLY 106 20.227 4.469 16.751 1.00 24.97
ATOM 770 N VAL 107 19.043 3.323 15.222 1.00 27.72
ATOM 771 CA VAL 107 18.612 4.522 14.529 1.00 25.88
ATOM 772 CB VAL 107 19.608 4.900 13.424 1.00 24.77
ATOM 773 CG1 VAL 107 19.527 3.891 12.307 1.00 24.43
ATOM 774 CG2 VAL 107 19.328 6.308 12.927 1.00 27.27
ATOM 775 C VAL 107 17.240 4.345 13.906 1.00 26.16
ATOM 776 O VAL 107 16.821 3.226 13.586 1.00 26.91
ATOM 777 N HIS 108 16.561 5.481 13.751 1.00 22.68
ATOM 778 CA HIS 108 15.239 5.558 13.176 1.00 21.88
ATOM 779 CB HIS 108 14.657 6.930 13.459 1.00 18.95
ATOM 780 CG HIS 108 13.354 7.185 12.773 1.00 18.23
ATOM 781 CD2 HIS 108 12.114 7.404 13.274 1.00 16.34
ATOM 782 ND1 HIS 108 13.233 7.242 11.399 1.00 15.06
ATOM 783 CE1 HIS 108 11.971 7.486 11.084 1.00 16.78
ATOM 784 NE2 HIS 108 11.272 7.588 12.203 1.00 18.04
ATOM 785 C HIS 108 15.336 5.338 11.687 1.00 24.47
ATOM 786 O HIS 108 15.754 6.225 10.945 1.00 28.53
ATOM 787 N SER 109 14.933 4.151 11.259 1.00 26.53
ATOM 788 CA SER 109 14.971 3.767 9.846 1.00 26.33
ATOM 789 CB SER 109 13.901 2.718 9.596 1.00 25.81
ATOM 790 OG SER 109 14.122 1.617 10.464 1.00 30.40
ATOM 791 C SER 109 14.800 4.902 8.855 1.00 24.40
ATOM 792 O SER 109 15.747 5.278 8.155 1.00 23.43
ATOM 793 N GLY 110 13.575 5.416 8.805 1.00 23.31
ATOM 794 CA GLY 110 13.244 6.506 7.921 1.00 25.96
ATOM 795 C GLY 110 14.392 7.453 7.742 1.00 26.60
ATOM 796 O GLY 110 15.010 7.515 6.689 1.00 27.43
ATOM 797 N PHE 111 14.693 8.183 8.798 1.00 24.09
ATOM 798 CA PHE 111 15.773 9.159 8.742 1.00 23.35
ATOM 799 CB PHE 111 16.105 9.663 10.139 1.00 22.88
ATOM 800 CG PHE 111 14.912 10.175 10.893 1.00 20.11
ATOM 801 CD1 PHE 111 13.822 10.729 10.213 1.00 16.75
ATOM 802 CD2 PHE 111 14.885 10.152 12.287 1.00 17.31
ATOM 803 CE1 PHE 111 12.731 11.251 10.926 1.00 17.67
ATOM 804 CE2 PHE 111 13.797 10.676 12.986 1.00 19.96
ATOM 805 CZ PHE 111 12.731 11.221 12.308 1.00 19.28
ATOM 806 C PHE 111 17.031 8.596 8.086 1.00 24.50
ATOM 807 O PHE 111 17.600 9.218 7.188 1.00 23.12
ATOM 808 N GLN 112 17.452 7.407 8.501 1.00 24.02
ATOM 809 CA GLN 112 18.649 6.815 7.921 1.00 26.60
ATOM 810 CB GLN 112 19.051 5.555 8.677 1.00 26.33
ATOM 811 CG GLN 112 20.212 4.850 8.021 1.00 26.25
ATOM 812 CD GLN 112 21.011 4.000 8.981 1.00 29.74
ATOM 813 OE1 GLN 112 21.443 2.894 8.639 1.00 35.31
ATOM 814 NE2 GLN 112 21.243 4.519 10.180 1.00 34.04
ATOM 815 C GLN 112 18.516 6.488 6.435 1.00 27.78
ATOM 816 O GLN 112 19.436 6.745 5.647 1.00 29.45
ATOM 817 N ASN 113 17.371 5.938 6.041 1.00 28.08
ATOM 818 CA ASN 113 17.175 5.596 4.639 1.00 28.43
ATOM 819 CB ASN 113 15.839 4.901 4.424 1.00 28.41
ATOM 820 CG ASN 113 15.871 3.451 4.863 1.00 30.04
ATOM 821 OD1 ASN 113 16.792 2.700 4.496 1.00 30.46
ATOM 822 ND2 ASN 113 14.865 3.040 5.643 1.00 28.40
ATOM 823 C ASN 113 17.233 6.841 3.803 1.00 28.99
ATOM 824 O ASN 113 17.865 6.880 2.759 1.00 33.51
ATOM 825 N ALA 114 16.554 7.869 4.279 1.00 28.45
ATOM 826 CA ALA 114 16.527 9.145 3.583 1.00 27.49
ATOM 827 CB ALA 114 15.801 10.171 4.405 1.00 26.03
ATOM 828 C ALA 114 17.950 9.609 3.343 1.00 27.35
ATOM 829 O ALA 114 18.299 10.023 2.241 1.00 29.80
ATOM 830 N TRP 115 18.767 9.532 4.391 1.00 23.62
ATOM 831 CA TRP 115 20.155 9.952 4.323 1.00 21.51
ATOM 832 CB TRP 115 20.875 9.730 5.652 1.00 21.64
ATOM 833 CG TRP 115 22.372 9.888 5.533 1.00 20.35
ATOM 834 CD2 TRP 115 23.087 10.970 4.903 1.00 19.79
ATOM 835 CE2 TRP 115 24.474 10.687 5.036 1.00 18.85
ATOM 836 CE3 TRP 115 22.691 12.146 4.247 1.00 17.80
ATOM 837 CD1 TRP 115 23.321 9.029 5.998 1.00 20.04
ATOM 838 NE1 TRP 115 24.593 9.500 5.700 1.00 17.55
ATOM 839 CZ2 TRP 115 25.456 11.537 4.543 1.00 17.95
ATOM 840 CZ3 TRP 115 23.672 12.994 3.756 1.00 18.83
ATOM 841 CH2 TRP 115 25.042 12.681 3.909 1.00 21.55
ATOM 842 C TRP 115 20.868 9.144 3.295 1.00 23.16
ATOM 843 O TRP 115 21.623 9.663 2.471 1.00 27.46
ATOM 844 N ASN 116 20.658 7.846 3.386 1.00 23.42
ATOM 845 CA ASN 116 21.304 6.953 2.465 1.00 24.21
ATOM 846 CB ASN 116 21.030 5.516 2.849 1.00 24.03
ATOM 847 CG ASN 116 21.676 5.150 4.132 1.00 29.29
ATOM 848 OD1 ASN 116 22.717 5.687 4.481 1.00 31.97
ATOM 849 ND2 ASN 116 21.080 4.218 4.844 1.00 33.01
ATOM 850 C ASN 116 20.836 7.172 1.052 1.00 23.56
ATOM 851 O ASN 116 21.567 6.937 0.093 1.00 22.94
ATOM 852 N GLU 117 19.604 7.614 0.897 1.00 20.73
ATOM 853 CA GLU 117 19.146 7.772 -0.454 1.00 22.57
ATOM 854 CB GLU 117 17.640 8.002 -0.509 1.00 21.79
ATOM 855 CG GLU 117 17.137 7.876 -1.925 1.00 27.82
ATOM 856 CD GLU 117 15.642 7.790 -2.017 1.00 28.18
ATOM 857 OE1 GLU 117 15.136 7.453 -3.110 1.00 28.56
ATOM 858 OE2 GLU 117 14.979 8.066 -1.000 1.00 27.41
ATOM 859 C GLU 117 19.849 8.891 -1.180 1.00 22.98
ATOM 860 O GLU 117 19.907 8.890 -2.413 1.00 22.77
ATOM 861 N ILE 118 20.433 9.812 -0.421 1.00 24.29
ATOM 862 CA ILE 118 21.056 10.989 -1.017 1.00 24.36
ATOM 863 CB ILE 118 20.160 12.210 -0.739 1.00 23.50
ATOM 864 CG2 ILE 118 18.679 11.913 -1.178 1.00 17.72
ATOM 865 CG1 ILE 118 20.229 12.521 0.768 1.00 23.92
ATOM 866 CD1 ILE 118 19.649 13.873 1.188 1.00 24.45
ATOM 867 C ILE 118 22.470 11.302 -0.500 1.00 27.82
ATOM 868 O ILE 118 23.083 12.301 -0.893 1.00 27.76
ATOM 869 N SER 119 22.989 10.455 0.384 1.00 28.69
ATOM 870 CA SER 119 24.321 10.676 0.972 1.00 31.82
ATOM 871 CB SER 119 24.707 9.487 1.842 1.00 31.79
ATOM 872 OG SER 119 24.796 8.321 1.064 1.00 31.33
ATOM 873 C SER 119 25.459 10.952 -0.015 1.00 31.63
ATOM 874 O SER 119 26.269 11.845 0.191 1.00 32.58
ATOM 875 N ALA 120 25.508 10.187 -1.095 1.00 30.64
ATOM 876 CA ALA 120 26.563 10.324 -2.104 1.00 31.31
ATOM 877 CB ALA 120 26.414 9.246 -3.178 1.00 32.77
ATOM 878 C ALA 120 26.633 11.678 -2.769 1.00 30.92
ATOM 879 O ALA 120 27.681 12.311 -2.810 1.00 32.49
ATOM 880 N ALA 121 25.499 12.115 -3.290 1.00 28.48
ATOM 881 CA ALA 121 25.396 13.411 -3.954 1.00 23.95
ATOM 882 CB ALA 121 24.007 13.605 -4.511 1.00 22.70
ATOM 883 C ALA 121 25.681 14.490 -2.958 1.00 23.32
ATOM 884 O ALA 121 26.352 15.466 -3.250 1.00 20.59
ATOM 885 N ALA 122 25.151 14.282 -1.765 1.00 23.65
ATOM 886 CA ALA 122 25.323 15.216 -0.668 1.00 25.49
ATOM 887 CB ALA 122 24.650 14.670 0.571 1.00 25.06
ATOM 888 C ALA 122 26.803 15.400 -0.407 1.00 26.41
ATOM 889 O ALA 122 27.327 16.507 -0.423 1.00 24.84
ATOM 890 N THR 123 27.481 14.285 -0.194 1.00 24.70
ATOM 891 CA THR 123 28.904 14.297 0.068 1.00 26.60
ATOM 892 CB THR 123 29.406 12.912 0.185 1.00 26.27
ATOM 893 OG1 THR 123 28.622 12.238 1.168 1.00 28.89
ATOM 894 CG2 THR 123 30.873 12.916 0.569 1.00 23.09
ATOM 895 C THR 123 29.748 14.970 -0.989 1.00 29.08
ATOM 896 O THR 123 30.545 15.869 -0.698 1.00 29.71
ATOM 897 N ALA 124 29.591 14.494 -2.213 1.00 29.51
ATOM 898 CA ALA 124 30.335 15.033 -3.317 1.00 30.04
ATOM 899 CB ALA 124 29.850 14.428 -4.590 1.00 28.86
ATOM 900 C ALA 124 30.214 16.536 -3.384 1.00 31.99
ATOM 901 O ALA 124 31.229 17.231 -3.494 1.00 32.89
ATOM 902 N ALA 125 28.978 17.032 -3.324 1.00 32.86
ATOM 903 CA ALA 125 28.732 18.470 -3.390 1.00 32.71
ATOM 904 CB ALA 125 27.257 18.762 -3.214 1.00 29.72
ATOM 905 C ALA 125 29.548 19.196 -2.316 1.00 36.27
ATOM 906 O ALA 125 30.168 20.230 -2.585 1.00 38.52
ATOM 907 N VAL 126 29.556 18.648 -1.106 1.00 37.28
ATOM 908 CA VAL 126 30.299 19.247 -0.015 1.00 37.15
ATOM 909 CB VAL 126 30.080 18.467 1.271 1.00 35.30
ATOM 910 CG1 VAL 126 30.905 19.081 2.376 1.00 31.71
ATOM 911 CG2 VAL 126 28.620 18.467 1.628 1.00 37.20
ATOM 912 C VAL 126 31.788 19.281 -0.316 1.00 39.89
ATOM 913 O VAL 126 32.500 20.211 0.078 1.00 39.87
ATOM 914 N ALA 127 32.263 18.246 -0.997 1.00 41.11
ATOM 915 CA ALA 127 33.666 18.176 -1.340 1.00 41.91
ATOM 916 CB ALA 127 33.964 16.836 -1.939 1.00 38.95
ATOM 917 C ALA 127 33.971 19.272 -2.336 1.00 42.35
ATOM 918 O ALA 127 34.782 20.147 -2.112 1.00 43.45
ATOM 919 N LYS 128 33.270 19.189 -3.449 1.00 42.74
ATOM 920 CA LYS 128 33.376 20.124 -4.552 1.00 45.06
ATOM 921 CB LYS 128 32.173 19.946 -5.493 1.00 46.03
ATOM 922 CG LYS 128 32.162 20.899 -6.664 1.00 45.30
ATOM 923 CD LYS 128 31.018 20.569 -7.617 1.00 43.76
ATOM 924 CE LYS 128 31.086 21.435 -8.866 1.00 42.90
ATOM 925 NZ LYS 128 30.055 21.041 -9.865 1.00 47.44
ATOM 926 C LYS 128 33.445 21.580 -4.124 1.00 47.18
ATOM 927 O LYS 128 34.218 22.365 -4.688 1.00 48.16
ATOM 928 N ALA 129 32.619 21.947 -3.149 1.00 46.89
ATOM 929 CA ALA 129 32.568 23.327 -2.681 1.00 48.47
ATOM 930 CB ALA 129 31.176 23.649 -2.202 1.00 50.70
ATOM 931 C ALA 129 33.556 23.554 -1.564 1.00 48.47
ATOM 932 O ALA 129 33.724 24.664 -1.069 1.00 49.52
ATOM 933 N ARG 130 34.216 22.481 -1.173 1.00 47.79
ATOM 934 CA ARG 130 35.177 22.533 -0.091 1.00 47.18
ATOM 935 CB ARG 130 35.056 21.238 0.708 1.00 46.27
ATOM 936 CG ARG 130 35.996 21.101 1.866 1.00 45.34
ATOM 937 CD ARG 130 35.303 21.237 3.216 1.00 45.42
ATOM 938 NE ARG 130 36.154 20.722 4.292 1.00 46.95
ATOM 939 CZ ARG 130 36.532 19.446 4.379 1.00 47.21
ATOM 940 NH1 ARG 130 36.125 18.578 3.455 1.00 49.67
ATOM 941 NH2 ARG 130 37.316 19.030 5.374 1.00 47.17
ATOM 942 C ARG 130 36.569 22.695 -0.688 1.00 47.32
ATOM 943 O ARG 130 37.481 23.265 -0.085 1.00 46.83
ATOM 944 N LYS 131 36.699 22.182 -1.900 1.00 47.02
ATOM 945 CA LYS 131 37.927 22.234 -2.665 1.00 47.60
ATOM 946 CB LYS 131 37.898 21.096 -3.683 1.00 48.24
ATOM 947 CG LYS 131 38.905 21.171 -4.797 1.00 49.97
ATOM 948 CD LYS 131 38.712 20.006 -5.773 1.00 51.12
ATOM 949 CE LYS 131 39.616 20.100 -7.012 1.00 54.18
ATOM 950 NZ LYS 131 41.091 20.138 -6.750 1.00 52.50
ATOM 951 C LYS 131 37.935 23.590 -3.358 1.00 46.50
ATOM 952 O LYS 131 38.974 24.205 -3.565 1.00 45.65
ATOM 953 N ALA 132 36.745 24.057 -3.703 1.00 46.55
ATOM 954 CA ALA 132 36.596 25.338 -4.366 1.00 46.83
ATOM 955 CB ALA 132 35.250 25.412 -5.040 1.00 46.52
ATOM 956 C ALA 132 36.734 26.481 -3.392 1.00 47.29
ATOM 957 O ALA 132 37.140 27.564 -3.742 1.00 48.72
ATOM 958 N ASN 133 36.393 26.237 -2.149 1.00 47.56
ATOM 959 CA ASN 133 36.475 27.290 -1.167 1.00 47.89
ATOM 960 CB ASN 133 35.073 27.831 -0.904 1.00 47.13
ATOM 961 CG ASN 133 34.379 28.258 -2.180 1.00 44.88
ATOM 962 OD1 ASN 133 34.762 29.251 -2.797 1.00 46.01
ATOM 963 ND2 ASN 133 33.365 27.501 -2.594 1.00 42.65
ATOM 964 C ASN 133 37.088 26.709 0.084 1.00 51.35
ATOM 965 O ASN 133 36.428 26.587 1.115 1.00 54.41
ATOM 966 N PRO 134 38.379 26.354 0.004 1.00 52.07
ATOM 967 CD PRO 134 39.241 26.711 -1.139 1.00 51.41
ATOM 968 CA PRO 134 39.175 25.765 1.082 1.00 51.27
ATOM 969 CB PRO 134 40.543 25.589 0.429 1.00 50.94
ATOM 970 CG PRO 134 40.598 26.756 -0.500 1.00 53.24
ATOM 971 C PRO 134 39.248 26.523 2.416 1.00 52.11
ATOM 972 O PRO 134 39.540 25.917 3.452 1.00 54.28
ATOM 973 N SER 135 38.995 27.829 2.417 1.00 51.79
ATOM 974 CA SER 135 39.048 28.568 3.681 1.00 49.81
ATOM 975 CB SER 135 39.238 30.065 3.427 1.00 48.81
ATOM 976 OG SER 135 38.140 30.602 2.716 1.00 44.30
ATOM 977 C SER 135 37.760 28.354 4.469 1.00 48.48
ATOM 978 O SER 135 37.769 28.345 5.707 1.00 48.39
ATOM 979 N PHE 136 36.660 28.178 3.734 1.00 46.17
ATOM 980 CA PHE 136 35.320 27.980 4.295 1.00 44.62
ATOM 981 CB PHE 136 34.335 27.829 3.135 1.00 44.25
ATOM 982 CG PHE 136 34.154 29.083 2.324 1.00 43.96
ATOM 983 CD1 PHE 136 35.249 29.862 1.966 1.00 44.47
ATOM 984 CD2 PHE 136 32.883 29.499 1.930 1.00 45.10
ATOM 985 CE1 PHE 136 35.079 31.039 1.232 1.00 45.46
ATOM 986 CE2 PHE 136 32.707 30.675 1.195 1.00 47.14
ATOM 987 CZ PHE 136 33.805 31.443 0.850 1.00 46.31
ATOM 988 C PHE 136 35.167 26.799 5.266 1.00 43.26
ATOM 989 O PHE 136 35.839 25.768 5.133 1.00 42.48
ATOM 990 N LYS 137 34.298 26.969 6.259 1.00 42.74
ATOM 991 CA LYS 137 34.016 25.918 7.240 1.00 45.31
ATOM 992 CB LYS 137 33.944 26.514 8.642 1.00 47.43
ATOM 993 CG LYS 137 33.075 27.753 8.724 1.00 50.73
ATOM 994 CD LYS 137 33.088 28.321 10.120 1.00 54.00
ATOM 995 CE LYS 137 32.294 29.614 10.197 1.00 56.52
ATOM 996 NZ LYS 137 32.838 30.691 9.310 1.00 54.83
ATOM 997 C LYS 137 32.664 25.315 6.843 1.00 43.52
ATOM 998 O LYS 137 31.980 25.863 5.978 1.00 43.49
ATOM 999 N VAL 138 32.271 24.207 7.467 1.00 40.21
ATOM 1000 CA VAL 138 31.014 23.569 7.100 1.00 39.86
ATOM 1001 CB VAL 138 31.236 22.126 6.677 1.00 39.80
ATOM 1002 CG1 VAL 138 30.044 21.660 5.858 1.00 37.32
ATOM 1003 CG2 VAL 138 32.524 21.995 5.912 1.00 38.61
ATOM 1004 C VAL 138 29.934 23.540 8.169 1.00 38.40
ATOM 1005 O VAL 138 30.222 23.430 9.357 1.00 39.54
ATOM 1006 N VAL 139 28.685 23.595 7.715 1.00 36.50
ATOM 1007 CA VAL 139 27.528 23.559 8.596 1.00 38.24
ATOM 1008 CB VAL 139 26.933 24.956 8.791 1.00 39.41
ATOM 1009 CG1 VAL 139 25.652 24.858 9.596 1.00 38.25
ATOM 1010 CG2 VAL 139 27.942 25.850 9.487 1.00 40.77
ATOM 1011 C VAL 139 26.437 22.642 8.051 1.00 36.49
ATOM 1012 O VAL 139 26.124 22.650 6.866 1.00 39.45
ATOM 1013 N SER 140 25.835 21.869 8.937 1.00 32.14
ATOM 1014 CA SER 140 24.792 20.936 8.552 1.00 29.04
ATOM 1015 CB SER 140 25.181 19.543 9.042 1.00 24.02
ATOM 1016 OG SER 140 24.210 18.589 8.703 1.00 29.12
ATOM 1017 C SER 140 23.510 21.413 9.222 1.00 28.02
ATOM 1018 O SER 140 23.397 21.358 10.441 1.00 27.82
ATOM 1019 N VAL 141 22.553 21.874 8.417 1.00 27.82
ATOM 1020 CA VAL 141 21.281 22.399 8.907 1.00 30.87
ATOM 1021 CB VAL 141 21.111 23.883 8.420 1.00 29.90
ATOM 1022 CG1 VAL 141 19.865 24.527 9.022 1.00 28.75
ATOM 1023 CG2 VAL 141 22.348 24.679 8.798 1.00 33.17
ATOM 1024 C VAL 141 20.074 21.550 8.485 1.00 33.03
ATOM 1025 O VAL 141 20.176 20.659 7.644 1.00 35.84
ATOM 1026 N GLY 142 18.933 21.848 9.104 1.00 32.70
ATOM 1027 CA GLY 142 17.686 21.152 8.838 1.00 30.09
ATOM 1028 C GLY 142 16.645 21.396 9.923 1.00 29.65
ATOM 1029 O GLY 142 16.960 21.411 11.111 1.00 31.37
ATOM 1030 N HIS 143 15.399 21.590 9.496 1.00 26.39
ATOM 1031 CA HIS 143 14.269 21.829 10.399 1.00 19.33
ATOM 1032 CB HIS 143 13.491 23.102 10.017 1.00 19.62
ATOM 1033 CG HIS 143 12.026 23.054 10.359 1.00 20.09
ATOM 1034 CD2 HIS 143 11.352 23.478 11.459 1.00 18.21
ATOM 1035 ND1 HIS 143 11.070 22.536 9.506 1.00 21.83
ATOM 1036 CE1 HIS 143 9.872 22.646 10.062 1.00 21.18
ATOM 1037 NE2 HIS 143 10.017 23.216 11.248 1.00 20.26
ATOM 1038 C HIS 143 13.315 20.672 10.321 1.00 18.40
ATOM 1039 O HIS 143 13.069 20.129 9.238 1.00 19.89
ATOM 1040 N SER 144 12.751 20.312 11.464 1.00 17.17
ATOM 1041 CA SER 144 11.805 19.233 11.467 1.00 16.24
ATOM 1042 CB SER 144 10.818 19.452 10.328 1.00 13.89
ATOM 1043 OG SER 144 9.608 18.743 10.515 1.00 21.42
ATOM 1044 C SER 144 12.578 17.950 11.231 1.00 17.37
ATOM 1045 O SER 144 13.698 17.746 11.721 1.00 17.35
ATOM 1046 N LEU 145 11.965 17.085 10.448 1.00 14.63
ATOM 1047 CA LEU 145 12.564 15.820 10.130 1.00 16.67
ATOM 1048 CB LEU 145 11.613 14.995 9.247 1.00 14.08
ATOM 1049 CG LEU 145 10.177 14.808 9.767 1.00 15.88
ATOM 1050 CD1 LEU 145 9.389 13.779 8.946 1.00 11.76
ATOM 1051 CD2 LEU 145 10.246 14.399 11.216 1.00 14.25
ATOM 1052 C LEU 145 13.834 16.173 9.373 1.00 19.50
ATOM 1053 O LEU 145 14.886 15.546 9.557 1.00 18.82
ATOM 1054 N GLY 146 13.733 17.204 8.537 1.00 19.28
ATOM 1055 CA GLY 146 14.882 17.635 7.770 1.00 21.26
ATOM 1056 C GLY 146 16.006 17.826 8.753 1.00 21.58
ATOM 1057 O GLY 146 17.161 17.608 8.433 1.00 24.44
ATOM 1058 N GLY 147 15.657 18.254 9.962 1.00 20.38
ATOM 1059 CA GLY 147 16.656 18.427 11.002 1.00 22.70
ATOM 1060 C GLY 147 17.203 17.074 11.425 1.00 23.22
ATOM 1061 O GLY 147 18.411 16.899 11.618 1.00 17.17
ATOM 1062 N ALA 148 16.295 16.107 11.569 1.00 24.83
ATOM 1063 CA ALA 148 16.682 14.750 11.955 1.00 26.11
ATOM 1064 CB ALA 148 15.499 13.795 11.842 1.00 26.21
ATOM 1065 C ALA 148 17.789 14.310 11.024 1.00 26.98
ATOM 1066 O ALA 148 18.944 14.236 11.415 1.00 26.77
ATOM 1067 N VAL 149 17.420 14.043 9.780 1.00 29.05
ATOM 1068 CA VAL 149 18.370 13.642 8.754 1.00 30.78
ATOM 1069 CB VAL 149 17.720 13.723 7.356 1.00 29.50
ATOM 1070 CG1 VAL 149 17.139 15.107 7.141 1.00 36.38
ATOM 1071 CG2 VAL 149 18.757 13.417 6.271 1.00 28.41
ATOM 1072 C VAL 149 19.631 14.520 8.758 1.00 32.09
ATOM 1073 O VAL 149 20.733 14.040 8.502 1.00 35.50
ATOM 1074 N ALA 150 19.476 15.808 9.052 1.00 31.81
ATOM 1075 CA ALA 150 20.627 16.720 9.050 1.00 31.91
ATOM 1076 CB ALA 150 20.188 18.142 9.407 1.00 31.19
ATOM 1077 C ALA 150 21.692 16.249 10.029 1.00 31.31
ATOM 1078 O ALA 150 22.876 16.480 9.838 1.00 31.99
ATOM 1079 N THR 151 21.262 15.571 11.075 1.00 31.04
ATOM 1080 CA THR 151 22.188 15.092 12.070 1.00 30.45
ATOM 1081 CB THR 151 21.442 14.612 13.316 1.00 31.26
ATOM 1082 OG1 THR 151 20.654 15.683 13.847 1.00 35.05
ATOM 1083 CG2 THR 151 22.430 14.179 14.369 1.00 31.46
ATOM 1084 C THR 151 23.070 13.961 11.573 1.00 30.95
ATOM 1085 O THR 151 24.291 14.020 11.709 1.00 30.80
ATOM 1086 N LEU 152 22.459 12.917 11.024 1.00 30.43
ATOM 1087 CA LEU 152 23.224 11.771 10.522 1.00 31.80
ATOM 1088 CB LEU 152 22.307 10.750 9.829 1.00 25.27
ATOM 1089 CG LEU 152 21.276 10.058 10.721 1.00 19.74
ATOM 1090 CD1 LEU 152 20.542 8.952 10.000 1.00 19.66
ATOM 1091 CD2 LEU 152 22.004 9.488 11.878 1.00 19.81
ATOM 1092 C LEU 152 24.246 12.282 9.527 1.00 33.94
ATOM 1093 O LEU 152 25.437 11.976 9.593 1.00 35.31
ATOM 1094 N ALA 153 23.754 13.101 8.617 1.00 33.85
ATOM 1095 CA ALA 153 24.590 13.692 7.597 1.00 33.67
ATOM 1096 CB ALA 153 23.795 14.716 6.823 1.00 36.30
ATOM 1097 C ALA 153 25.798 14.339 8.247 1.00 32.20
ATOM 1098 O ALA 153 26.896 14.239 7.745 1.00 33.42
ATOM 1099 N GLY 154 25.596 14.994 9.381 1.00 33.09
ATOM 1100 CA GLY 154 26.712 15.630 10.048 1.00 33.71
ATOM 1101 C GLY 154 27.637 14.570 10.590 1.00 34.42
ATOM 1102 O GLY 154 28.830 14.546 10.293 1.00 34.11
ATOM 1103 N ALA 155 27.065 13.682 11.394 1.00 35.16
ATOM 1104 CA ALA 155 27.815 12.602 12.020 1.00 37.79
ATOM 1105 CB ALA 155 26.855 11.612 12.635 1.00 36.39
ATOM 1106 C ALA 155 28.685 11.902 10.991 1.00 37.41
ATOM 1107 O ALA 155 29.902 11.805 11.120 1.00 36.20
ATOM 1108 N ASN 156 28.030 11.406 9.960 1.00 37.07
ATOM 1109 CA ASN 156 28.730 10.721 8.917 1.00 36.66
ATOM 1110 CB ASN 156 27.715 10.144 7.937 1.00 35.94
ATOM 1111 CG ASN 156 27.170 8.813 8.410 1.00 37.19
ATOM 1112 OD1 ASN 156 27.823 7.782 8.257 1.00 41.36
ATOM 1113 ND2 ASN 156 25.986 8.828 9.007 1.00 38.22
ATOM 1114 C ASN 156 29.732 11.640 8.235 1.00 38.00
ATOM 1115 O ASN 156 30.905 11.295 8.117 1.00 39.70
ATOM 1116 N LEU 157 29.302 12.825 7.815 1.00 36.33
ATOM 1117 CA LEU 157 30.231 13.721 7.125 1.00 30.00
ATOM 1118 CB LEU 157 29.610 15.104 6.865 1.00 26.41
ATOM 1119 CG LEU 157 29.181 15.474 5.424 1.00 26.33
ATOM 1120 CD1 LEU 157 30.071 14.777 4.412 1.00 24.41
ATOM 1121 CD2 LEU 157 27.761 15.069 5.167 1.00 26.08
ATOM 1122 C LEU 157 31.477 13.857 7.973 1.00 30.24
ATOM 1123 O LEU 157 32.600 13.744 7.491 1.00 29.89
ATOM 1124 N ARG 158 31.255 14.058 9.261 1.00 29.77
ATOM 1125 CA ARG 158 32.345 14.195 10.194 1.00 32.79
ATOM 1126 CB ARG 158 31.820 14.192 11.645 1.00 33.07
ATOM 1127 CG ARG 158 30.861 15.331 12.023 1.00 32.85
ATOM 1128 CD ARG 158 31.088 15.717 13.489 1.00 35.95
ATOM 1129 NE ARG 158 29.886 16.070 14.260 1.00 38.94
ATOM 1130 CZ ARG 158 29.346 17.287 14.342 1.00 39.79
ATOM 1131 NH1 ARG 158 28.259 17.469 15.082 1.00 32.39
ATOM 1132 NH2 ARG 158 29.875 18.323 13.686 1.00 40.89
ATOM 1133 C ARG 158 33.320 13.037 9.998 1.00 35.98
ATOM 1134 O ARG 158 34.485 13.250 9.709 1.00 40.19
ATOM 1135 N ILE 159 32.835 11.805 10.136 1.00 36.99
ATOM 1136 CA ILE 159 33.708 10.632 10.014 1.00 36.27
ATOM 1137 CB ILE 159 32.963 9.267 10.118 1.00 36.24
ATOM 1138 CG2 ILE 159 32.042 9.259 11.325 1.00 30.70
ATOM 1139 CG1 ILE 159 32.157 8.994 8.855 1.00 41.45
ATOM 1140 CD1 ILE 159 31.199 7.805 9.010 1.00 48.56
ATOM 1141 C ILE 159 34.394 10.661 8.692 1.00 37.10
ATOM 1142 O ILE 159 35.466 10.073 8.528 1.00 39.04
ATOM 1143 N GLY 160 33.759 11.341 7.744 1.00 39.77
ATOM 1144 CA GLY 160 34.332 11.467 6.421 1.00 45.59
ATOM 1145 C GLY 160 35.558 12.367 6.464 1.00 49.14
ATOM 1146 O GLY 160 36.290 12.512 5.491 1.00 49.04
ATOM 1147 N GLY 161 35.797 12.974 7.614 1.00 49.77
ATOM 1148 CA GLY 161 36.940 13.847 7.732 1.00 49.94
ATOM 1149 C GLY 161 36.429 15.241 7.928 1.00 48.63
ATOM 1150 O GLY 161 36.604 15.837 8.982 1.00 51.78
ATOM 1151 N THR 162 35.758 15.752 6.914 1.00 45.61
ATOM 1152 CA THR 162 35.227 17.098 6.992 1.00 43.42
ATOM 1153 CB THR 162 34.318 17.391 5.828 1.00 43.68
ATOM 1154 OG1 THR 162 33.204 18.157 6.298 1.00 46.49
ATOM 1155 CG2 THR 162 33.854 16.105 5.195 1.00 44.15
ATOM 1156 C THR 162 34.454 17.394 8.273 1.00 42.04
ATOM 1157 O THR 162 33.408 16.801 8.550 1.00 40.79
ATOM 1158 N PRO 163 34.988 18.311 9.082 1.00 41.64
ATOM 1159 CD PRO 163 36.376 18.797 8.979 1.00 39.68
ATOM 1160 CA PRO 163 34.380 18.718 10.346 1.00 42.23
ATOM 1161 CB PRO 163 35.560 19.279 11.134 1.00 43.75
ATOM 1162 CG PRO 163 36.438 19.839 10.054 1.00 44.13
ATOM 1163 C PRO 163 33.229 19.703 10.165 1.00 40.16
ATOM 1164 O PRO 163 33.223 20.565 9.275 1.00 37.11
ATOM 1165 N LEU 164 32.267 19.559 11.067 1.00 40.42
ATOM 1166 CA LEU 164 31.031 20.322 11.051 1.00 38.72
ATOM 1167 CB LEU 164 29.883 19.434 10.632 1.00 35.75
ATOM 1168 CG LEU 164 29.691 19.202 9.166 1.00 33.13
ATOM 1169 CD1 LEU 164 28.838 17.977 9.033 1.00 37.44
ATOM 1170 CD2 LEU 164 29.047 20.412 8.519 1.00 29.26
ATOM 1171 C LEU 164 30.579 20.917 12.349 1.00 38.94
ATOM 1172 O LEU 164 31.271 20.888 13.368 1.00 42.10
ATOM 1173 N ASP 165 29.346 21.407 12.258 1.00 40.82
ATOM 1174 CA ASP 165 28.593 22.013 13.326 1.00 39.97
ATOM 1175 CB ASP 165 28.921 23.491 13.494 1.00 41.99
ATOM 1176 CG ASP 165 29.864 23.743 14.638 1.00 45.08
ATOM 1177 OD1 ASP 165 29.907 22.907 15.571 1.00 47.09
ATOM 1178 OD2 ASP 165 30.550 24.786 14.610 1.00 46.42
ATOM 1179 C ASP 165 27.193 21.882 12.776 1.00 38.60
ATOM 1180 O ASP 165 26.868 22.451 11.732 1.00 40.54
ATOM 1181 N ILE 166 26.368 21.124 13.486 1.00 33.75
ATOM 1182 CA ILE 166 24.983 20.891 13.096 1.00 30.12
ATOM 1183 CB ILE 166 24.577 19.449 13.398 1.00 27.81
ATOM 1184 CG2 ILE 166 23.165 19.180 12.900 1.00 28.09
ATOM 1185 CG1 ILE 166 25.580 18.499 12.731 1.00 27.25
ATOM 1186 CD1 ILE 166 25.524 17.076 13.276 1.00 16.32
ATOM 1187 C ILE 166 24.012 21.806 13.813 1.00 28.19
ATOM 1188 O ILE 166 24.072 21.950 15.037 1.00 28.70
ATOM 1189 N TYR 167 23.120 22.409 13.030 1.00 26.22
ATOM 1190 CA TYR 167 22.085 23.310 13.529 1.00 29.29
ATOM 1191 CB TYR 167 22.225 24.703 12.915 1.00 29.15
ATOM 1192 CG TYR 167 23.295 25.581 13.541 1.00 29.82
ATOM 1193 CD1 TYR 167 24.634 25.451 13.193 1.00 31.46
ATOM 1194 CE1 TYR 167 25.618 26.271 13.778 1.00 33.38
ATOM 1195 CD2 TYR 167 22.956 26.547 14.490 1.00 31.23
ATOM 1196 CE2 TYR 167 23.917 27.362 15.082 1.00 31.33
ATOM 1197 CZ TYR 167 25.250 27.224 14.726 1.00 32.94
ATOM 1198 OH TYR 167 26.198 28.032 15.329 1.00 33.66
ATOM 1199 C TYR 167 20.713 22.756 13.155 1.00 30.93
ATOM 1200 O TYR 167 20.380 22.642 11.977 1.00 31.73
ATOM 1201 N THR 168 19.917 22.429 14.163 1.00 33.46
ATOM 1202 CA THR 168 18.592 21.866 13.950 1.00 34.18
ATOM 1203 CB THR 168 18.564 20.428 14.427 1.00 34.56
ATOM 1204 OG1 THR 168 19.567 20.266 15.439 1.00 36.47
ATOM 1205 CG2 THR 168 18.855 19.470 13.274 1.00 35.43
ATOM 1206 C THR 168 17.494 22.643 14.659 1.00 34.34
ATOM 1207 O THR 168 17.718 23.310 15.667 1.00 37.07
ATOM 1208 N TYR 169 16.294 22.535 14.106 1.00 33.59
ATOM 1209 CA TYR 169 15.132 23.227 14.615 1.00 31.03
ATOM 1210 CB TYR 169 14.839 24.434 13.726 1.00 26.08
ATOM 1211 CG TYR 169 16.037 25.332 13.525 1.00 27.60
ATOM 1212 CD1 TYR 169 17.109 24.930 12.739 1.00 30.90
ATOM 1213 CE1 TYR 169 18.217 25.762 12.533 1.00 31.53
ATOM 1214 CD2 TYR 169 16.094 26.587 14.112 1.00 28.88
ATOM 1215 CE2 TYR 169 17.191 27.438 13.916 1.00 31.16
ATOM 1216 CZ TYR 169 18.254 27.023 13.116 1.00 33.28
ATOM 1217 OH TYR 169 19.323 27.867 12.846 1.00 32.19
ATOM 1218 C TYR 169 13.924 22.322 14.662 1.00 32.69
ATOM 1219 O TYR 169 13.402 21.904 13.631 1.00 31.23
ATOM 1220 N GLY 170 13.486 22.034 15.879 1.00 32.73
ATOM 1221 CA GLY 170 12.320 21.196 16.067 1.00 31.89
ATOM 1222 C GLY 170 12.510 19.828 15.448 1.00 31.16
ATOM 1223 O GLY 170 11.592 19.282 14.834 1.00 30.48
ATOM 1224 N SER 171 13.709 19.271 15.601 1.00 29.94
ATOM 1225 CA SER 171 13.999 17.953 15.043 1.00 29.41
ATOM 1226 CB SER 171 15.504 17.797 14.760 1.00 31.96
ATOM 1227 OG SER 171 16.189 17.109 15.813 1.00 28.95
ATOM 1228 C SER 171 13.568 16.897 16.050 1.00 27.43
ATOM 1229 O SER 171 13.529 17.136 17.262 1.00 30.66
ATOM 1230 N PRO 172 13.209 15.723 15.565 1.00 24.19
ATOM 1231 CD PRO 172 12.982 15.315 14.175 1.00 24.16
ATOM 1232 CA PRO 172 12.799 14.693 16.513 1.00 25.27
ATOM 1233 CB PRO 172 11.906 13.802 15.667 1.00 22.48
ATOM 1234 CG PRO 172 12.570 13.861 14.337 1.00 26.34
ATOM 1235 C PRO 172 14.071 13.995 17.006 1.00 26.93
ATOM 1236 O PRO 172 15.165 14.277 16.481 1.00 25.21
ATOM 1237 N ARG 173 13.950 13.122 18.015 1.00 24.87
ATOM 1238 CA ARG 173 15.117 12.387 18.509 1.00 19.89
ATOM 1239 CB ARG 173 14.741 11.481 19.651 1.00 16.72
ATOM 1240 CG ARG 173 14.075 12.137 20.827 1.00 13.01
ATOM 1241 CD ARG 173 14.184 11.214 22.011 1.00 18.41
ATOM 1242 NE ARG 173 13.775 11.943 23.200 1.00 22.47
ATOM 1243 CZ ARG 173 12.516 12.251 23.482 1.00 22.00
ATOM 1244 NH1 ARG 173 12.229 12.936 24.577 1.00 18.05
ATOM 1245 NH2 ARG 173 11.545 11.841 22.684 1.00 23.39
ATOM 1246 C ARG 173 15.482 11.515 17.327 1.00 20.52
ATOM 1247 O ARG 173 14.565 11.012 16.689 1.00 21.15
ATOM 1248 N VAL 174 16.770 11.306 17.024 1.00 22.47
ATOM 1249 CA VAL 174 17.135 10.497 15.827 1.00 25.15
ATOM 1250 CB VAL 174 18.144 11.313 14.881 1.00 23.04
ATOM 1251 CG1 VAL 174 18.336 12.735 15.425 1.00 22.73
ATOM 1252 CG2 VAL 174 19.469 10.612 14.772 1.00 24.59
ATOM 1253 C VAL 174 17.621 9.020 16.017 1.00 26.28
ATOM 1254 O VAL 174 17.560 8.223 15.067 1.00 26.79
ATOM 1255 N GLY 175 18.063 8.650 17.225 1.00 30.71
ATOM 1256 CA GLY 175 18.513 7.281 17.478 1.00 32.37
ATOM 1257 C GLY 175 18.428 6.893 18.942 1.00 32.98
ATOM 1258 O GLY 175 17.602 7.406 19.686 1.00 35.87
ATOM 1259 N ASN 176 19.285 5.971 19.359 1.00 30.86
ATOM 1260 CA ASN 176 19.302 5.535 20.747 1.00 31.73
ATOM 1261 CB ASN 176 19.361 4.007 20.873 1.00 28.19
ATOM 1262 CG ASN 176 20.700 3.408 20.428 1.00 30.78
ATOM 1263 OD1 ASN 176 20.915 2.203 20.552 1.00 28.67
ATOM 1264 ND2 ASN 176 21.592 4.238 19.907 1.00 35.19
ATOM 1265 C ASN 176 20.475 6.123 21.488 1.00 32.41
ATOM 1266 O ASN 176 21.370 6.776 20.926 1.00 32.01
ATOM 1267 N THR 177 20.452 5.888 22.779 1.00 31.20
ATOM 1268 CA THR 177 21.497 6.359 23.660 1.00 35.00
ATOM 1269 CB THR 177 21.510 5.478 24.861 1.00 33.97
ATOM 1270 OG1 THR 177 20.534 4.443 24.654 1.00 40.81
ATOM 1271 CG2 THR 177 21.141 6.276 26.090 1.00 34.76
ATOM 1272 C THR 177 22.889 6.430 23.069 1.00 36.41
ATOM 1273 O THR 177 23.475 7.492 23.025 1.00 38.30
ATOM 1274 N GLN 178 23.423 5.309 22.605 1.00 36.58
ATOM 1275 CA GLN 178 24.776 5.343 22.045 1.00 38.33
ATOM 1276 CB GLN 178 25.380 3.928 21.855 1.00 37.70
ATOM 1277 CG GLN 178 24.401 2.867 21.458 1.00 37.39
ATOM 1278 CD GLN 178 23.795 2.165 22.668 1.00 38.56
ATOM 1279 OE1 GLN 178 24.497 1.494 23.415 1.00 37.40
ATOM 1280 NE2 GLN 178 22.486 2.312 22.857 1.00 36.48
ATOM 1281 C GLN 178 24.882 6.124 20.748 1.00 36.06
ATOM 1282 O GLN 178 25.936 6.708 20.448 1.00 35.15
ATOM 1283 N LEU 179 23.821 6.163 19.963 1.00 31.34
ATOM 1284 CA LEU 179 23.972 6.929 18.753 1.00 31.50
ATOM 1285 CB LEU 179 22.786 6.691 17.840 1.00 28.66
ATOM 1286 CG LEU 179 22.690 7.609 16.631 1.00 27.94
ATOM 1287 CD1 LEU 179 23.680 7.207 15.572 1.00 29.06
ATOM 1288 CD2 LEU 179 21.302 7.507 16.072 1.00 33.99
ATOM 1289 C LEU 179 24.061 8.405 19.175 1.00 34.01
ATOM 1290 O LEU 179 25.032 9.111 18.845 1.00 33.60
ATOM 1291 N ALA 180 23.061 8.856 19.935 1.00 34.29
ATOM 1292 CA ALA 180 23.013 10.245 20.375 1.00 34.25
ATOM 1293 CB ALA 180 21.878 10.440 21.312 1.00 31.38
ATOM 1294 C ALA 180 24.291 10.650 21.067 1.00 35.98
ATOM 1295 O ALA 180 24.757 11.779 20.946 1.00 39.76
ATOM 1296 N ALA 181 24.831 9.722 21.836 1.00 34.88
ATOM 1297 CA ALA 181 26.039 9.966 22.583 1.00 31.64
ATOM 1298 CB ALA 181 26.369 8.771 23.380 1.00 28.24
ATOM 1299 C ALA 181 27.139 10.249 21.632 1.00 32.31
ATOM 1300 O ALA 181 27.818 11.261 21.713 1.00 30.45
ATOM 1301 N PHE 182 27.311 9.311 20.729 1.00 33.84
ATOM 1302 CA PHE 182 28.338 9.409 19.721 1.00 37.67
ATOM 1303 CB PHE 182 28.038 8.399 18.634 1.00 35.24
ATOM 1304 CG PHE 182 29.006 8.421 17.513 1.00 32.23
ATOM 1305 CD1 PHE 182 28.581 8.744 16.230 1.00 35.37
ATOM 1306 CD2 PHE 182 30.325 8.027 17.709 1.00 31.11
ATOM 1307 CE1 PHE 182 29.453 8.658 15.150 1.00 35.57
ATOM 1308 CE2 PHE 182 31.203 7.937 16.629 1.00 32.35
ATOM 1309 CZ PHE 182 30.764 8.252 15.351 1.00 34.57
ATOM 1310 C PHE 182 28.412 10.809 19.123 1.00 40.35
ATOM 1311 O PHE 182 29.431 11.500 19.216 1.00 40.89
ATOM 1312 N VAL 183 27.308 11.226 18.527 1.00 38.30
ATOM 1313 CA VAL 183 27.235 12.529 17.917 1.00 39.18
ATOM 1314 CB VAL 183 25.866 12.741 17.393 1.00 40.72
ATOM 1315 CG1 VAL 183 25.790 14.058 16.649 1.00 39.25
ATOM 1316 CG2 VAL 183 25.507 11.552 16.530 1.00 37.39
ATOM 1317 C VAL 183 27.534 13.621 18.919 1.00 38.88
ATOM 1318 O VAL 183 28.213 14.600 18.622 1.00 39.49
ATOM 1319 N SER 184 27.014 13.457 20.119 1.00 37.57
ATOM 1320 CA SER 184 27.251 14.452 21.126 1.00 37.92
ATOM 1321 CB SER 184 26.554 14.053 22.413 1.00 34.90
ATOM 1322 OG SER 184 25.169 14.191 22.253 1.00 31.35
ATOM 1323 C SER 184 28.740 14.599 21.370 1.00 40.95
ATOM 1324 O SER 184 29.262 15.713 21.453 1.00 43.53
ATOM 1325 N ASN 185 29.417 13.463 21.459 1.00 42.49
ATOM 1326 CA ASN 185 30.838 13.428 21.749 1.00 43.30
ATOM 1327 CB ASN 185 31.129 12.154 22.478 1.00 46.28
ATOM 1328 CG ASN 185 30.120 11.904 23.542 1.00 51.12
ATOM 1329 OD1 ASN 185 29.977 12.708 24.455 1.00 51.56
ATOM 1330 ND2 ASN 185 29.390 10.809 23.430 1.00 56.16
ATOM 1331 C ASN 185 31.714 13.526 20.554 1.00 43.01
ATOM 1332 O ASN 185 32.880 13.173 20.592 1.00 43.68
ATOM 1333 N GLN 186 31.137 13.981 19.469 1.00 39.81
ATOM 1334 CA GLN 186 31.917 14.138 18.283 1.00 39.03
ATOM 1335 CB GLN 186 31.058 13.959 17.041 1.00 39.39
ATOM 1336 CG GLN 186 31.029 12.570 16.476 1.00 36.32
ATOM 1337 CD GLN 186 30.509 12.574 15.068 1.00 35.86
ATOM 1338 OE1 GLN 186 30.888 11.732 14.255 1.00 39.13
ATOM 1339 NE2 GLN 186 29.627 13.522 14.764 1.00 35.55
ATOM 1340 C GLN 186 32.454 15.541 18.324 1.00 39.42
ATOM 1341 O GLN 186 31.866 16.437 18.926 1.00 41.40
ATOM 1342 N ALA 187 33.605 15.724 17.710 1.00 38.62
ATOM 1343 CA ALA 187 34.194 17.042 17.666 1.00 38.34
ATOM 1344 CB ALA 187 35.438 17.030 16.788 1.00 37.50
ATOM 1345 C ALA 187 33.118 17.938 17.069 1.00 39.50
ATOM 1346 O ALA 187 32.374 17.532 16.150 1.00 38.37
ATOM 1347 N GLY 188 33.012 19.146 17.615 1.00 39.42
ATOM 1348 CA GLY 188 32.009 20.067 17.114 1.00 38.11
ATOM 1349 C GLY 188 30.737 20.118 17.950 1.00 35.77
ATOM 1350 O GLY 188 30.613 19.421 18.987 1.00 35.70
ATOM 1351 N GLY 189 29.789 20.939 17.500 1.00 33.30
ATOM 1352 CA GLY 189 28.548 21.082 18.230 1.00 34.89
ATOM 1353 C GLY 189 27.280 20.762 17.472 1.00 35.30
ATOM 1354 O GLY 189 27.213 20.843 16.233 1.00 36.66
ATOM 1355 N GLU 190 26.271 20.397 18.261 1.00 34.26
ATOM 1356 CA GLU 190 24.926 20.036 17.802 1.00 30.13
ATOM 1357 CB GLU 190 24.568 18.609 18.250 1.00 29.94
ATOM 1358 CG GLU 190 25.555 17.509 17.877 1.00 30.77
ATOM 1359 CD GLU 190 26.936 17.697 18.461 1.00 32.53
ATOM 1360 OE1 GLU 190 27.052 17.995 19.673 1.00 34.56
ATOM 1361 OE2 GLU 190 27.905 17.523 17.695 1.00 32.05
ATOM 1362 C GLU 190 23.988 20.987 18.552 1.00 25.52
ATOM 1363 O GLU 190 23.506 20.631 19.645 1.00 25.60
ATOM 1364 N TYR 191 23.747 22.178 18.012 1.00 23.84
ATOM 1365 CA TYR 191 22.868 23.104 18.689 1.00 26.80
ATOM 1366 CB TYR 191 23.167 24.539 18.295 1.00 29.71
ATOM 1367 CG TYR 191 24.614 24.924 18.207 1.00 32.88
ATOM 1368 CD1 TYR 191 25.198 25.723 19.184 1.00 33.47
ATOM 1369 CE1 TYR 191 26.526 26.132 19.071 1.00 33.90
ATOM 1370 CD2 TYR 191 25.390 24.537 17.119 1.00 35.22
ATOM 1371 CE2 TYR 191 26.702 24.938 16.997 1.00 38.47
ATOM 1372 CZ TYR 191 27.265 25.737 17.967 1.00 37.34
ATOM 1373 OH TYR 191 28.561 26.167 17.795 1.00 41.52
ATOM 1374 C TYR 191 21.482 22.789 18.188 1.00 27.01
ATOM 1375 O TYR 191 21.111 23.265 17.118 1.00 30.00
ATOM 1376 N ARG 192 20.721 21.970 18.912 1.00 23.47
ATOM 1377 CA ARG 192 19.363 21.697 18.466 1.00 23.80
ATOM 1378 CB ARG 192 18.966 20.213 18.555 1.00 23.43
ATOM 1379 CG ARG 192 18.894 19.585 19.917 1.00 26.56
ATOM 1380 CD ARG 192 18.227 18.215 19.805 1.00 29.50
ATOM 1381 NE ARG 192 17.268 17.990 20.884 1.00 30.31
ATOM 1382 CZ ARG 192 17.598 17.672 22.130 1.00 32.33
ATOM 1383 NH1 ARG 192 16.654 17.501 23.046 1.00 33.74
ATOM 1384 NH2 ARG 192 18.863 17.492 22.449 1.00 35.07
ATOM 1385 C ARG 192 18.448 22.558 19.290 1.00 22.85
ATOM 1386 O ARG 192 18.488 22.540 20.542 1.00 22.61
ATOM 1387 N VAL 193 17.670 23.352 18.554 1.00 20.52
ATOM 1388 CA VAL 193 16.726 24.306 19.117 1.00 24.71
ATOM 1389 CB VAL 193 16.760 25.635 18.307 1.00 25.59
ATOM 1390 CG1 VAL 193 15.771 26.629 18.888 1.00 23.22
ATOM 1391 CG2 VAL 193 18.191 26.227 18.280 1.00 26.47
ATOM 1392 C VAL 193 15.284 23.782 19.106 1.00 28.05
ATOM 1393 O VAL 193 14.845 23.158 18.111 1.00 32.43
ATOM 1394 N THR 194 14.564 24.055 20.205 1.00 29.93
ATOM 1395 CA THR 194 13.166 23.659 20.383 1.00 32.80
ATOM 1396 CB THR 194 13.009 22.586 21.494 1.00 33.39
ATOM 1397 OG1 THR 194 13.570 23.052 22.734 1.00 34.60
ATOM 1398 CG2 THR 194 13.700 21.316 21.074 1.00 35.77
ATOM 1399 C THR 194 12.253 24.844 20.728 1.00 33.73
ATOM 1400 O THR 194 12.623 25.790 21.432 1.00 34.75
ATOM 1401 N ASN 195 11.022 24.762 20.258 1.00 33.87
ATOM 1402 CA ASN 195 10.076 25.835 20.492 1.00 36.33
ATOM 1403 CB ASN 195 9.586 26.345 19.132 1.00 36.77
ATOM 1404 CG ASN 195 8.726 27.574 19.232 1.00 37.55
ATOM 1405 OD1 ASN 195 8.810 28.352 20.193 1.00 32.92
ATOM 1406 ND2 ASN 195 7.896 27.773 18.213 1.00 33.28
ATOM 1407 C ASN 195 8.911 25.443 21.395 1.00 36.22
ATOM 1408 O ASN 195 8.031 24.664 21.002 1.00 33.64
ATOM 1409 N ALA 196 8.931 25.979 22.617 1.00 37.74
ATOM 1410 CA ALA 196 7.864 25.730 23.592 1.00 39.36
ATOM 1411 CB ALA 196 6.670 26.666 23.303 1.00 42.05
ATOM 1412 C ALA 196 7.377 24.271 23.670 1.00 40.34
ATOM 1413 O ALA 196 8.135 23.373 24.112 1.00 43.70
ATOM 1414 N LYS 197 6.117 24.049 23.265 1.00 38.99
ATOM 1415 CA LYS 197 5.503 22.726 23.292 1.00 35.84
ATOM 1416 CB LYS 197 4.137 22.823 23.920 1.00 33.03
ATOM 1417 CG LYS 197 4.182 23.206 25.348 1.00 36.10
ATOM 1418 CD LYS 197 2.794 23.473 25.836 1.00 37.51
ATOM 1419 CE LYS 197 2.783 23.551 27.342 1.00 39.85
ATOM 1420 NZ LYS 197 3.163 22.223 27.944 1.00 43.96
ATOM 1421 C LYS 197 5.366 22.066 21.930 1.00 36.67
ATOM 1422 O LYS 197 4.373 21.373 21.679 1.00 38.07
ATOM 1423 N ASP 198 6.351 22.295 21.055 1.00 34.49
ATOM 1424 CA ASP 198 6.384 21.686 19.717 1.00 32.29
ATOM 1425 CB ASP 198 7.708 22.032 19.013 1.00 29.09
ATOM 1426 CG ASP 198 7.882 21.302 17.701 1.00 26.03
ATOM 1427 OD1 ASP 198 7.062 20.409 17.414 1.00 23.23
ATOM 1428 OD2 ASP 198 8.845 21.615 16.969 1.00 21.49
ATOM 1429 C ASP 198 6.333 20.180 20.033 1.00 33.21
ATOM 1430 O ASP 198 7.153 19.676 20.809 1.00 36.31
ATOM 1431 N PRO 199 5.384 19.438 19.442 1.00 31.90
ATOM 1432 CD PRO 199 4.208 19.890 18.679 1.00 33.69
ATOM 1433 CA PRO 199 5.302 18.004 19.740 1.00 31.80
ATOM 1434 CB PRO 199 3.859 17.676 19.408 1.00 30.13
ATOM 1435 CG PRO 199 3.581 18.575 18.252 1.00 30.92
ATOM 1436 C PRO 199 6.266 17.016 19.090 1.00 34.31
ATOM 1437 O PRO 199 6.313 15.856 19.490 1.00 37.03
ATOM 1438 N VAL 200 7.038 17.451 18.102 1.00 32.02
ATOM 1439 CA VAL 200 7.952 16.525 17.429 1.00 33.55
ATOM 1440 CB VAL 200 8.140 16.879 15.896 1.00 31.45
ATOM 1441 CG1 VAL 200 7.679 18.286 15.609 1.00 26.24
ATOM 1442 CG2 VAL 200 9.603 16.708 15.471 1.00 26.07
ATOM 1443 C VAL 200 9.314 16.342 18.080 1.00 36.82
ATOM 1444 O VAL 200 9.900 15.254 18.026 1.00 36.73
ATOM 1445 N PRO 201 9.843 17.398 18.704 1.00 39.43
ATOM 1446 CD PRO 201 9.432 18.809 18.677 1.00 40.66
ATOM 1447 CA PRO 201 11.152 17.259 19.341 1.00 38.84
ATOM 1448 CB PRO 201 11.466 18.682 19.778 1.00 35.83
ATOM 1449 CG PRO 201 10.765 19.504 18.729 1.00 38.37
ATOM 1450 C PRO 201 11.121 16.280 20.507 1.00 38.72
ATOM 1451 O PRO 201 12.136 16.029 21.137 1.00 41.44
ATOM 1452 N ARG 202 9.944 15.735 20.791 1.00 38.20
ATOM 1453 CA ARG 202 9.810 14.778 21.865 1.00 33.59
ATOM 1454 CB ARG 202 8.833 15.287 22.943 1.00 31.21
ATOM 1455 CG ARG 202 7.619 16.100 22.527 1.00 26.21
ATOM 1456 CD ARG 202 6.651 16.096 23.704 1.00 28.07
ATOM 1457 NE ARG 202 5.721 17.221 23.771 1.00 32.08
ATOM 1458 CZ ARG 202 4.543 17.282 23.145 1.00 31.23
ATOM 1459 NH1 ARG 202 3.782 18.357 23.293 1.00 33.90
ATOM 1460 NH2 ARG 202 4.112 16.286 22.372 1.00 30.66
ATOM 1461 C ARG 202 9.406 13.419 21.309 1.00 32.52
ATOM 1462 O ARG 202 8.807 12.575 22.004 1.00 35.11
ATOM 1463 N LEU 203 9.773 13.204 20.048 1.00 29.82
ATOM 1464 CA LEU 203 9.481 11.946 19.380 1.00 25.27
ATOM 1465 CB LEU 203 8.263 12.104 18.460 1.00 24.76
ATOM 1466 CG LEU 203 6.964 12.372 19.219 1.00 25.85
ATOM 1467 CD1 LEU 203 5.883 12.703 18.207 1.00 20.27
ATOM 1468 CD2 LEU 203 6.585 11.156 20.109 1.00 19.33
ATOM 1469 C LEU 203 10.706 11.514 18.572 1.00 21.60
ATOM 1470 O LEU 203 11.471 12.352 18.074 1.00 19.99
ATOM 1471 N PRO 204 10.948 10.200 18.489 1.00 18.93
ATOM 1472 CD PRO 204 11.765 9.581 17.427 1.00 17.45
ATOM 1473 CA PRO 204 10.098 9.203 19.144 1.00 20.16
ATOM 1474 CB PRO 204 10.572 7.888 18.516 1.00 21.98
ATOM 1475 CG PRO 204 11.007 8.301 17.141 1.00 16.12
ATOM 1476 C PRO 204 10.234 9.215 20.685 1.00 18.91
ATOM 1477 O PRO 204 11.176 9.789 21.239 1.00 15.54
ATOM 1478 N PRO 205 9.299 8.554 21.388 1.00 19.35
ATOM 1479 CD PRO 205 8.213 7.738 20.823 1.00 20.56
ATOM 1480 CA PRO 205 9.278 8.477 22.850 1.00 21.21
ATOM 1481 CB PRO 205 8.091 7.555 23.131 1.00 19.58
ATOM 1482 CG PRO 205 7.212 7.756 21.945 1.00 19.74
ATOM 1483 C PRO 205 10.561 7.919 23.442 1.00 24.31
ATOM 1484 O PRO 205 11.210 7.055 22.842 1.00 27.43
ATOM 1485 N LEU 206 10.924 8.411 24.624 1.00 24.15
ATOM 1486 CA LEU 206 12.115 7.908 25.284 1.00 26.76
ATOM 1487 CB LEU 206 12.329 8.538 26.663 1.00 25.72
ATOM 1488 CG LEU 206 12.816 9.977 26.831 1.00 28.25
ATOM 1489 CD1 LEU 206 13.274 10.191 28.268 1.00 26.97
ATOM 1490 CD2 LEU 206 13.970 10.252 25.873 1.00 29.72
ATOM 1491 C LEU 206 11.864 6.431 25.493 1.00 28.70
ATOM 1492 O LEU 206 12.751 5.607 25.218 1.00 32.61
ATOM 1493 N ILE 207 10.654 6.101 25.968 1.00 30.14
ATOM 1494 CA ILE 207 10.296 4.714 26.248 1.00 31.14
ATOM 1495 CB ILE 207 8.799 4.556 26.569 1.00 28.18
ATOM 1496 CG2 ILE 207 8.463 5.299 27.868 1.00 26.68
ATOM 1497 CG1 ILE 207 7.966 5.038 25.387 1.00 26.98
ATOM 1498 CD1 ILE 207 6.479 4.718 25.510 1.00 32.66
ATOM 1499 C ILE 207 10.653 3.767 25.103 1.00 33.97
ATOM 1500 O ILE 207 10.739 2.543 25.309 1.00 34.93
ATOM 1501 N PHE 208 10.864 4.317 23.904 1.00 34.47
ATOM 1502 CA PHE 208 11.213 3.473 22.771 1.00 33.35
ATOM 1503 CB PHE 208 10.242 3.743 21.597 1.00 33.97
ATOM 1504 CG PHE 208 8.801 3.322 21.882 1.00 37.81
ATOM 1505 CD1 PHE 208 7.728 4.050 21.361 1.00 36.85
ATOM 1506 CD2 PHE 208 8.518 2.225 22.716 1.00 38.73
ATOM 1507 CE1 PHE 208 6.393 3.706 21.672 1.00 35.52
ATOM 1508 CE2 PHE 208 7.182 1.871 23.032 1.00 38.10
ATOM 1509 CZ PHE 208 6.124 2.623 22.504 1.00 37.46
ATOM 1510 C PHE 208 12.702 3.627 22.394 1.00 30.91
ATOM 1511 O PHE 208 13.085 3.557 21.230 1.00 33.03
ATOM 1512 N GLY 209 13.530 3.813 23.422 1.00 27.41
ATOM 1513 CA GLY 209 14.977 3.902 23.270 1.00 27.70
ATOM 1514 C GLY 209 15.564 5.177 22.712 1.00 29.53
ATOM 1515 O GLY 209 16.769 5.436 22.851 1.00 29.48
ATOM 1516 N TYR 210 14.718 5.962 22.055 1.00 29.56
ATOM 1517 CA TYR 210 15.154 7.198 21.451 1.00 29.10
ATOM 1518 CB TYR 210 14.054 7.729 20.544 1.00 28.00
ATOM 1519 CG TYR 210 13.904 6.928 19.253 1.00 28.06
ATOM 1520 CD1 TYR 210 14.611 7.283 18.093 1.00 26.44
ATOM 1521 CE1 TYR 210 14.454 6.564 16.884 1.00 25.01
ATOM 1522 CD2 TYR 210 13.043 5.829 19.181 1.00 22.49
ATOM 1523 CE2 TYR 210 12.881 5.099 17.980 1.00 24.49
ATOM 1524 CZ TYR 210 13.585 5.477 16.839 1.00 26.12
ATOM 1525 OH TYR 210 13.400 4.789 15.654 1.00 28.95
ATOM 1526 C TYR 210 15.564 8.211 22.506 1.00 30.17
ATOM 1527 O TYR 210 14.879 8.419 23.530 1.00 31.17
ATOM 1528 N ARG 211 16.716 8.818 22.230 1.00 29.17
ATOM 1529 CA ARG 211 17.357 9.805 23.082 1.00 27.79
ATOM 1530 CB ARG 211 18.591 9.177 23.707 1.00 26.76
ATOM 1531 CG ARG 211 18.308 7.871 24.418 1.00 30.07
ATOM 1532 CD ARG 211 17.554 8.140 25.688 1.00 28.13
ATOM 1533 NE ARG 211 16.471 7.194 25.872 1.00 31.44
ATOM 1534 CZ ARG 211 16.342 6.426 26.945 1.00 37.43
ATOM 1535 NH1 ARG 211 17.240 6.511 27.912 1.00 38.25
ATOM 1536 NH2 ARG 211 15.323 5.576 27.053 1.00 39.38
ATOM 1537 C ARG 211 17.789 10.976 22.206 1.00 29.96
ATOM 1538 O ARG 211 17.767 10.860 20.969 1.00 35.98
ATOM 1539 N HIS 212 18.196 12.080 22.837 1.00 28.81
ATOM 1540 CA HIS 212 18.632 13.264 22.116 1.00 27.59
ATOM 1541 CB HIS 212 17.826 14.468 22.586 1.00 25.29
ATOM 1542 CG HIS 212 16.632 14.782 21.733 1.00 21.16
ATOM 1543 CD2 HIS 212 15.329 14.985 22.051 1.00 18.56
ATOM 1544 ND1 HIS 212 16.727 14.996 20.374 1.00 19.29
ATOM 1545 CE1 HIS 212 15.538 15.314 19.892 1.00 19.37
ATOM 1546 NE2 HIS 212 14.671 15.313 20.889 1.00 18.19
ATOM 1547 C HIS 212 20.115 13.592 22.258 1.00 29.89
ATOM 1548 O HIS 212 20.858 12.902 22.948 1.00 32.67
ATOM 1549 N THR 213 20.533 14.653 21.568 1.00 32.51
ATOM 1550 CA THR 213 21.915 15.151 21.609 1.00 34.41
ATOM 1551 CB THR 213 22.434 15.671 20.251 1.00 35.45
ATOM 1552 OG1 THR 213 21.376 16.357 19.565 1.00 36.52
ATOM 1553 CG2 THR 213 22.991 14.545 19.421 1.00 38.69
ATOM 1554 C THR 213 21.929 16.359 22.518 1.00 35.02
ATOM 1555 O THR 213 21.008 17.165 22.518 1.00 33.04
ATOM 1556 N SER 214 22.998 16.511 23.266 1.00 35.79
ATOM 1557 CA SER 214 23.068 17.627 24.161 1.00 37.40
ATOM 1558 CB SER 214 23.556 17.122 25.514 1.00 37.51
ATOM 1559 OG SER 214 23.560 18.133 26.501 1.00 42.60
ATOM 1560 C SER 214 23.990 18.694 23.575 1.00 37.69
ATOM 1561 O SER 214 24.931 18.390 22.842 1.00 39.04
ATOM 1562 N PRO 215 23.699 19.968 23.857 1.00 36.66
ATOM 1563 CD PRO 215 24.595 21.111 23.606 1.00 36.17
ATOM 1564 CA PRO 215 22.562 20.383 24.677 1.00 35.16
ATOM 1565 CB PRO 215 23.032 21.699 25.252 1.00 34.57
ATOM 1566 CG PRO 215 23.761 22.286 24.085 1.00 34.47
ATOM 1567 C PRO 215 21.286 20.568 23.879 1.00 35.43
ATOM 1568 O PRO 215 21.129 20.049 22.773 1.00 34.55
ATOM 1569 N GLU 216 20.389 21.341 24.469 1.00 36.14
ATOM 1570 CA GLU 216 19.101 21.651 23.885 1.00 35.35
ATOM 1571 CB GLU 216 18.015 20.782 24.504 1.00 34.93
ATOM 1572 CG GLU 216 16.632 21.260 24.130 1.00 31.58
ATOM 1573 CD GLU 216 15.537 20.628 24.973 1.00 34.98
ATOM 1574 OE1 GLU 216 15.723 19.485 25.463 1.00 41.04
ATOM 1575 OE2 GLU 216 14.474 21.272 25.133 1.00 34.46
ATOM 1576 C GLU 216 18.804 23.099 24.211 1.00 33.66
ATOM 1577 O GLU 216 18.693 23.478 25.375 1.00 32.94
ATOM 1578 N TYR 217 18.686 23.913 23.179 1.00 33.09
ATOM 1579 CA TYR 217 18.392 25.326 23.361 1.00 34.95
ATOM 1580 CB TYR 217 19.200 26.134 22.364 1.00 34.01
ATOM 1581 CG TYR 217 20.689 25.910 22.489 1.00 35.10
ATOM 1582 CD1 TYR 217 21.300 24.776 21.947 1.00 35.08
ATOM 1583 CE1 TYR 217 22.681 24.583 22.053 1.00 33.58
ATOM 1584 CD2 TYR 217 21.495 26.841 23.148 1.00 36.42
ATOM 1585 CE2 TYR 217 22.869 26.653 23.263 1.00 33.49
ATOM 1586 CZ TYR 217 23.453 25.523 22.710 1.00 32.43
ATOM 1587 OH TYR 217 24.803 25.332 22.805 1.00 31.62
ATOM 1588 C TYR 217 16.904 25.537 23.158 1.00 35.97
ATOM 1589 O TYR 217 16.422 25.725 22.050 1.00 37.11
ATOM 1590 N TRP 218 16.175 25.475 24.260 1.00 33.94
ATOM 1591 CA TRP 218 14.720 25.597 24.245 1.00 33.34
ATOM 1592 CB TRP 218 14.125 24.729 25.356 1.00 35.12
ATOM 1593 CG TRP 218 12.660 24.733 25.456 1.00 31.18
ATOM 1594 CD2 TRP 218 11.908 24.884 26.658 1.00 27.18
ATOM 1595 CE2 TRP 218 10.545 24.649 26.342 1.00 27.19
ATOM 1596 CE3 TRP 218 12.252 25.190 27.981 1.00 26.15
ATOM 1597 CD1 TRP 218 11.755 24.437 24.467 1.00 32.33
ATOM 1598 NE1 TRP 218 10.474 24.377 24.997 1.00 29.27
ATOM 1599 CZ2 TRP 218 9.536 24.706 27.299 1.00 23.07
ATOM 1600 CZ3 TRP 218 11.253 25.246 28.925 1.00 28.57
ATOM 1601 CH2 TRP 218 9.909 25.005 28.582 1.00 27.07
ATOM 1602 C TRP 218 14.215 27.006 24.385 1.00 32.24
ATOM 1603 O TRP 218 14.706 27.785 25.218 1.00 30.36
ATOM 1604 N LEU 219 13.223 27.316 23.553 1.00 32.03
ATOM 1605 CA LEU 219 12.606 28.635 23.539 1.00 30.96
ATOM 1606 CB LEU 219 12.153 28.992 22.130 1.00 24.86
ATOM 1607 CG LEU 219 13.219 29.035 21.047 1.00 22.19
ATOM 1608 CD1 LEU 219 12.604 29.413 19.702 1.00 23.26
ATOM 1609 CD2 LEU 219 14.294 30.024 21.451 1.00 23.32
ATOM 1610 C LEU 219 11.413 28.623 24.477 1.00 32.11
ATOM 1611 O LEU 219 10.383 28.003 24.199 1.00 31.03
ATOM 1612 N SER 220 11.574 29.320 25.591 1.00 34.19
ATOM 1613 CA SER 220 10.558 29.391 26.622 1.00 38.20
ATOM 1614 CB SER 220 11.232 29.675 27.975 1.00 40.51
ATOM 1615 OG SER 220 10.283 29.951 28.990 1.00 42.19
ATOM 1616 C SER 220 9.519 30.468 26.290 1.00 41.27
ATOM 1617 O SER 220 9.816 31.686 26.314 1.00 43.55
ATOM 1618 N GLY 221 8.309 30.004 25.974 1.00 42.71
ATOM 1619 CA GLY 221 7.228 30.901 25.630 1.00 44.34
ATOM 1620 C GLY 221 5.993 30.125 25.231 1.00 47.07
ATOM 1621 O GLY 221 5.641 29.130 25.880 1.00 48.33
ATOM 1622 N SER 222 5.335 30.570 24.162 1.00 47.78
ATOM 1623 CA SER 222 4.119 29.914 23.686 1.00 48.39
ATOM 1624 CB SER 222 2.896 30.819 23.912 1.00 48.99
ATOM 1625 OG SER 222 3.179 32.187 23.647 1.00 46.46
ATOM 1626 C SER 222 4.209 29.520 22.208 1.00 51.09
ATOM 1627 O SER 222 4.290 28.326 21.861 1.00 55.36
ATOM 1628 N ASP 223 4.181 30.530 21.340 1.00 51.73
ATOM 1629 CA ASP 223 4.269 30.334 19.895 1.00 51.72
ATOM 1630 CB ASP 223 3.077 29.475 19.432 1.00 46.28
ATOM 1631 CG ASP 223 2.848 29.517 17.926 1.00 44.22
ATOM 1632 OD1 ASP 223 3.763 29.198 17.126 1.00 46.70
ATOM 1633 OD2 ASP 223 1.719 29.867 17.539 1.00 38.38
ATOM 1634 C ASP 223 4.337 31.703 19.161 1.00 54.87
ATOM 1635 O ASP 223 3.716 32.693 19.587 1.00 55.13
ATOM 1636 N LYS 224 5.158 31.733 18.104 1.00 56.32
ATOM 1637 CA LYS 224 5.412 32.878 17.221 1.00 53.02
ATOM 1638 CB LYS 224 4.626 34.108 17.650 1.00 52.74
ATOM 1639 CG LYS 224 3.137 33.957 17.404 1.00 56.80
ATOM 1640 CD LYS 224 2.804 33.708 15.933 1.00 57.03
ATOM 1641 CE LYS 224 1.290 33.534 15.740 1.00 53.19
ATOM 1642 NZ LYS 224 0.831 33.475 14.322 1.00 50.36
ATOM 1643 C LYS 224 6.880 33.251 16.992 1.00 50.71
ATOM 1644 O LYS 224 7.801 32.547 17.395 1.00 49.91
ATOM 1645 N ILE 225 7.077 34.397 16.364 1.00 48.53
ATOM 1646 CA ILE 225 8.392 34.884 15.931 1.00 48.98
ATOM 1647 CB ILE 225 8.157 35.792 14.699 1.00 47.91
ATOM 1648 CG2 ILE 225 9.461 36.175 14.035 1.00 50.91
ATOM 1649 CG1 ILE 225 7.292 35.068 13.686 1.00 50.09
ATOM 1650 CD1 ILE 225 6.841 35.963 12.570 1.00 52.31
ATOM 1651 C ILE 225 9.461 35.593 16.803 1.00 48.42
ATOM 1652 O ILE 225 10.666 35.340 16.627 1.00 47.48
ATOM 1653 N ASP 226 9.046 36.449 17.733 1.00 46.35
ATOM 1654 CA ASP 226 9.998 37.258 18.509 1.00 42.89
ATOM 1655 CB ASP 226 9.318 38.569 18.839 1.00 44.72
ATOM 1656 CG ASP 226 8.058 38.357 19.657 1.00 50.94
ATOM 1657 OD1 ASP 226 7.807 37.187 20.044 1.00 51.85
ATOM 1658 OD2 ASP 226 7.327 39.343 19.919 1.00 51.91
ATOM 1659 C ASP 226 10.749 36.823 19.770 1.00 40.29
ATOM 1660 O ASP 226 10.907 37.650 20.674 1.00 36.29
ATOM 1661 N TYR 227 11.241 35.592 19.864 1.00 38.05
ATOM 1662 CA TYR 227 11.970 35.268 21.087 1.00 35.65
ATOM 1663 CB TYR 227 12.205 33.787 21.223 1.00 31.14
ATOM 1664 CG TYR 227 10.979 32.944 21.408 1.00 23.94
ATOM 1665 CD1 TYR 227 10.268 32.458 20.310 1.00 22.52
ATOM 1666 CE1 TYR 227 9.253 31.538 20.467 1.00 18.28
ATOM 1667 CD2 TYR 227 10.619 32.502 22.673 1.00 22.37
ATOM 1668 CE2 TYR 227 9.607 31.579 22.834 1.00 20.84
ATOM 1669 CZ TYR 227 8.937 31.099 21.724 1.00 20.72
ATOM 1670 OH TYR 227 7.991 30.116 21.863 1.00 24.72
ATOM 1671 C TYR 227 13.324 35.961 21.059 1.00 34.93
ATOM 1672 O TYR 227 13.749 36.474 20.012 1.00 35.23
ATOM 1673 N THR 228 14.002 35.971 22.207 1.00 33.89
ATOM 1674 CA THR 228 15.312 36.619 22.306 1.00 33.29
ATOM 1675 CB THR 228 15.188 38.000 22.909 1.00 29.80
ATOM 1676 OG1 THR 228 14.804 37.879 24.284 1.00 24.02
ATOM 1677 CG2 THR 228 14.135 38.777 22.157 1.00 22.64
ATOM 1678 C THR 228 16.226 35.795 23.189 1.00 34.79
ATOM 1679 O THR 228 15.765 34.869 23.840 1.00 38.39
ATOM 1680 N ILE 229 17.509 36.142 23.241 1.00 34.44
ATOM 1681 CA ILE 229 18.451 35.355 24.030 1.00 37.75
ATOM 1682 CB ILE 229 19.835 36.044 24.181 1.00 39.09
ATOM 1683 CG2 ILE 229 20.937 34.987 23.989 1.00 32.52
ATOM 1684 CG1 ILE 229 19.995 37.178 23.154 1.00 42.61
ATOM 1685 CD1 ILE 229 21.396 37.800 23.068 1.00 45.25
ATOM 1686 C ILE 229 17.932 35.044 25.414 1.00 39.33
ATOM 1687 O ILE 229 18.148 33.960 25.952 1.00 39.91
ATOM 1688 N ASN 230 17.195 35.991 25.971 1.00 42.05
ATOM 1689 CA ASN 230 16.663 35.840 27.318 1.00 43.15
ATOM 1690 CB ASN 230 16.427 37.216 27.905 1.00 44.81
ATOM 1691 CG ASN 230 17.722 37.982 28.064 1.00 46.86
ATOM 1692 OD1 ASN 230 17.718 39.201 28.182 1.00 44.52
ATOM 1693 ND2 ASN 230 18.849 37.258 28.079 1.00 48.08
ATOM 1694 C ASN 230 15.428 34.990 27.469 1.00 42.18
ATOM 1695 O ASN 230 14.925 34.809 28.572 1.00 41.84
ATOM 1696 N ASP 231 14.938 34.470 26.358 1.00 40.88
ATOM 1697 CA ASP 231 13.763 33.623 26.385 1.00 40.09
ATOM 1698 CB ASP 231 12.848 33.968 25.219 1.00 44.81
ATOM 1699 CG ASP 231 12.537 35.435 25.148 1.00 45.26
ATOM 1700 OD1 ASP 231 12.038 35.875 24.085 1.00 43.82
ATOM 1701 OD2 ASP 231 12.793 36.137 26.153 1.00 48.49
ATOM 1702 C ASP 231 14.259 32.200 26.220 1.00 38.80
ATOM 1703 O ASP 231 13.479 31.276 26.031 1.00 36.46
ATOM 1704 N VAL 232 15.574 32.029 26.263 1.00 37.12
ATOM 1705 CA VAL 232 16.147 30.706 26.100 1.00 36.25
ATOM 1706 CB VAL 232 17.434 30.762 25.227 1.00 34.82
ATOM 1707 CG1 VAL 232 18.128 29.393 25.227 1.00 30.44
ATOM 1708 CG2 VAL 232 17.070 31.144 23.803 1.00 30.02
ATOM 1709 C VAL 232 16.449 29.999 27.428 1.00 37.79
ATOM 1710 O VAL 232 16.680 30.642 28.469 1.00 39.93
ATOM 1711 N LYS 233 16.400 28.668 27.384 1.00 37.60
ATOM 1712 CA LYS 233 16.703 27.827 28.532 1.00 36.16
ATOM 1713 CB LYS 233 15.435 27.145 29.060 1.00 37.40
ATOM 1714 CG LYS 233 14.297 28.080 29.473 1.00 39.89
ATOM 1715 CD LYS 233 14.501 28.672 30.863 1.00 43.04
ATOM 1716 CE LYS 233 13.258 29.447 31.323 1.00 46.34
ATOM 1717 NZ LYS 233 11.986 28.648 31.284 1.00 47.35
ATOM 1718 C LYS 233 17.658 26.776 27.955 1.00 37.10
ATOM 1719 O LYS 233 17.608 26.484 26.757 1.00 40.45
ATOM 1720 N VAL 234 18.520 26.213 28.795 1.00 35.45
ATOM 1721 CA VAL 234 19.472 25.203 28.346 1.00 31.04
ATOM 1722 CB VAL 234 20.904 25.760 28.310 1.00 27.95
ATOM 1723 CG1 VAL 234 21.888 24.667 27.894 1.00 27.27
ATOM 1724 CG2 VAL 234 20.965 26.936 27.354 1.00 25.26
ATOM 1725 C VAL 234 19.438 23.990 29.257 1.00 32.55
ATOM 1726 O VAL 234 19.851 24.045 30.425 1.00 33.04
ATOM 1727 N CYS 235 18.938 22.895 28.694 1.00 35.25
ATOM 1728 CA CYS 235 18.781 21.619 29.391 1.00 38.31
ATOM 1729 C CYS 235 19.860 20.670 28.841 1.00 37.75
ATOM 1730 O CYS 235 20.025 20.522 27.620 1.00 39.24
ATOM 1731 CB CYS 235 17.342 21.121 29.116 1.00 36.40
ATOM 1732 SG CYS 235 16.413 22.533 28.396 1.00 49.33
ATOM 1733 N GLU 236 20.631 20.055 29.732 1.00 37.70
ATOM 1734 CA GLU 236 21.677 19.147 29.274 1.00 37.30
ATOM 1735 CB GLU 236 23.026 19.544 29.886 1.00 35.33
ATOM 1736 CG GLU 236 23.437 21.008 29.670 1.00 38.61
ATOM 1737 CD GLU 236 24.837 21.314 30.211 1.00 37.96
ATOM 1738 OE1 GLU 236 25.080 22.448 30.690 1.00 34.90
ATOM 1739 OE2 GLU 236 25.699 20.416 30.147 1.00 34.80
ATOM 1740 C GLU 236 21.332 17.687 29.612 1.00 37.87
ATOM 1741 O GLU 236 20.639 17.415 30.606 1.00 40.11
ATOM 1742 N GLY 237 21.785 16.753 28.769 1.00 36.80
ATOM 1743 CA GLY 237 21.513 15.337 28.998 1.00 32.73
ATOM 1744 C GLY 237 20.680 14.648 27.905 1.00 30.90
ATOM 1745 O GLY 237 19.797 15.260 27.294 1.00 29.19
ATOM 1746 N ALA 238 20.954 13.361 27.688 1.00 32.95
ATOM 1747 CA ALA 238 20.284 12.512 26.691 1.00 32.90
ATOM 1748 CB ALA 238 20.903 11.097 26.705 1.00 31.25
ATOM 1749 C ALA 238 18.788 12.396 26.924 1.00 34.16
ATOM 1750 O ALA 238 17.994 12.484 25.972 1.00 37.39
ATOM 1751 N ALA 239 18.426 12.178 28.194 1.00 32.51
ATOM 1752 CA ALA 239 17.028 12.037 28.623 1.00 33.61
ATOM 1753 CB ALA 239 16.777 10.624 29.152 1.00 30.52
ATOM 1754 C ALA 239 16.661 13.076 29.691 1.00 33.09
ATOM 1755 O ALA 239 16.689 12.802 30.887 1.00 38.01
ATOM 1756 N ASN 240 16.314 14.271 29.229 1.00 29.73
ATOM 1757 CA ASN 240 15.958 15.402 30.079 1.00 28.09
ATOM 1758 CB ASN 240 17.042 16.481 29.956 1.00 28.69
ATOM 1759 CG ASN 240 16.675 17.762 30.674 1.00 29.76
ATOM 1760 OD1 ASN 240 15.498 18.095 30.837 1.00 29.65
ATOM 1761 ND2 ASN 240 17.682 18.498 31.097 1.00 32.23
ATOM 1762 C ASN 240 14.614 15.959 29.593 1.00 27.56
ATOM 1763 O ASN 240 14.485 16.335 28.421 1.00 32.63
ATOM 1764 N LEU 241 13.622 16.051 30.473 1.00 25.68
ATOM 1765 CA LEU 241 12.315 16.546 30.048 1.00 25.18
ATOM 1766 CB LEU 241 11.268 15.505 30.374 1.00 20.15
ATOM 1767 CG LEU 241 11.654 14.108 29.910 1.00 20.13
ATOM 1768 CD1 LEU 241 10.667 13.096 30.438 1.00 19.46
ATOM 1769 CD2 LEU 241 11.712 14.082 28.394 1.00 7.75
ATOM 1770 C LEU 241 11.957 17.837 30.735 1.00 28.61
ATOM 1771 O LEU 241 10.792 18.178 30.881 1.00 31.35
ATOM 1772 N GLN 242 12.974 18.550 31.177 1.00 31.34
ATOM 1773 CA GLN 242 12.761 19.798 31.875 1.00 31.71
ATOM 1774 CB GLN 242 14.002 20.174 32.671 1.00 32.22
ATOM 1775 CG GLN 242 14.327 19.140 33.687 1.00 35.19
ATOM 1776 CD GLN 242 13.107 18.781 34.520 1.00 36.20
ATOM 1777 OE1 GLN 242 12.710 19.531 35.413 1.00 38.50
ATOM 1778 NE2 GLN 242 12.503 17.634 34.225 1.00 37.34
ATOM 1779 C GLN 242 12.478 20.864 30.863 1.00 31.94
ATOM 1780 O GLN 242 12.335 22.027 31.213 1.00 33.10
ATOM 1781 N CYS 243 12.386 20.468 29.604 1.00 34.24
ATOM 1782 CA CYS 243 12.139 21.444 28.583 1.00 35.22
ATOM 1783 C CYS 243 11.105 21.019 27.577 1.00 34.96
ATOM 1784 O CYS 243 9.971 20.709 27.949 1.00 34.17
ATOM 1785 CB CYS 243 13.470 21.795 27.949 1.00 37.75
ATOM 1786 SG CYS 243 14.547 22.600 29.193 1.00 41.04
ATOM 1787 N ASN 244 11.465 21.029 26.301 1.00 32.89
ATOM 1788 CA ASN 244 10.493 20.637 25.280 1.00 32.64
ATOM 1789 CB ASN 244 11.122 20.572 23.886 1.00 32.57
ATOM 1790 CG ASN 244 10.185 19.952 22.854 1.00 30.96
ATOM 1791 OD1 ASN 244 10.293 18.757 22.521 1.00 27.02
ATOM 1792 ND2 ASN 244 9.245 20.759 22.352 1.00 27.59
ATOM 1793 C ASN 244 9.911 19.277 25.623 1.00 34.40
ATOM 1794 O ASN 244 8.724 19.175 25.935 1.00 36.59
ATOM 1795 N GLY 245 10.741 18.235 25.548 1.00 33.08
ATOM 1796 CA GLY 245 10.274 16.910 25.907 1.00 30.30
ATOM 1797 C GLY 245 9.784 17.150 27.307 1.00 30.36
ATOM 1798 O GLY 245 10.491 17.757 28.110 1.00 32.61
ATOM 1799 N GLY 246 8.584 16.696 27.616 1.00 29.59
ATOM 1800 CA GLY 246 8.062 16.949 28.942 1.00 29.91
ATOM 1801 C GLY 246 6.897 17.915 28.813 1.00 30.92
ATOM 1802 O GLY 246 6.141 18.122 29.771 1.00 28.51
ATOM 1803 N THR 247 6.751 18.512 27.626 1.00 31.78
ATOM 1804 CA THR 247 5.646 19.431 27.364 1.00 32.55
ATOM 1805 CB THR 247 6.039 20.521 26.378 1.00 29.13
ATOM 1806 OG1 THR 247 6.538 19.917 25.181 1.00 34.76
ATOM 1807 CG2 THR 247 7.099 21.402 26.966 1.00 28.24
ATOM 1808 C THR 247 4.496 18.637 26.748 1.00 35.15
ATOM 1809 O THR 247 4.673 17.468 26.373 1.00 32.84
ATOM 1810 N LEU 248 3.329 19.273 26.629 1.00 37.01
ATOM 1811 CA LEU 248 2.164 18.600 26.070 1.00 36.63
ATOM 1812 CB LEU 248 1.197 18.251 27.191 1.00 31.69
ATOM 1813 CG LEU 248 1.729 17.092 28.023 1.00 30.56
ATOM 1814 CD1 LEU 248 0.903 16.929 29.279 1.00 27.80
ATOM 1815 CD2 LEU 248 1.715 15.829 27.176 1.00 26.69
ATOM 1816 C LEU 248 1.433 19.380 24.990 1.00 37.81
ATOM 1817 O LEU 248 1.881 20.452 24.550 1.00 38.54
ATOM 1818 N GLY 249 0.314 18.818 24.541 1.00 38.01
ATOM 1819 CA GLY 249 -0.462 19.488 23.520 1.00 39.37
ATOM 1820 C GLY 249 -0.059 19.107 22.106 1.00 38.76
ATOM 1821 O GLY 249 0.797 18.238 21.873 1.00 37.04
ATOM 1822 N LEU 250 -0.679 19.795 21.159 1.00 38.22
ATOM 1823 CA LEU 250 -0.458 19.553 19.755 1.00 38.05
ATOM 1824 CB LEU 250 -1.698 18.844 19.208 1.00 35.50
ATOM 1825 CG LEU 250 -1.516 17.814 18.109 1.00 33.40
ATOM 1826 CD1 LEU 250 -2.861 17.207 17.780 1.00 36.98
ATOM 1827 CD2 LEU 250 -0.908 18.472 16.897 1.00 34.84
ATOM 1828 C LEU 250 -0.265 20.916 19.091 1.00 36.02
ATOM 1829 O LEU 250 -0.939 21.238 18.099 1.00 36.86
ATOM 1830 N ASP 251 0.667 21.703 19.629 1.00 34.80
ATOM 1831 CA ASP 251 0.918 23.027 19.088 1.00 33.81
ATOM 1832 CB ASP 251 1.550 23.921 20.160 1.00 31.99
ATOM 1833 CG ASP 251 1.572 25.398 19.764 1.00 33.18
ATOM 1834 OD1 ASP 251 2.033 26.236 20.581 1.00 31.63
ATOM 1835 OD2 ASP 251 1.131 25.724 18.638 1.00 35.47
ATOM 1836 C ASP 251 1.804 22.963 17.847 1.00 33.35
ATOM 1837 O ASP 251 2.873 23.571 17.806 1.00 36.22
ATOM 1838 N ILE 252 1.336 22.233 16.836 1.00 32.44
ATOM 1839 CA ILE 252 2.057 22.086 15.582 1.00 32.27
ATOM 1840 CB ILE 252 1.186 21.361 14.523 1.00 33.71
ATOM 1841 CG2 ILE 252 1.772 21.563 13.132 1.00 33.09
ATOM 1842 CG1 ILE 252 1.076 19.862 14.853 1.00 35.60
ATOM 1843 CD1 ILE 252 0.206 19.058 13.909 1.00 35.69
ATOM 1844 C ILE 252 2.481 23.443 15.028 1.00 33.10
ATOM 1845 O ILE 252 3.450 23.533 14.270 1.00 36.41
ATOM 1846 N ASP 253 1.762 24.496 15.402 1.00 33.30
ATOM 1847 CA ASP 253 2.099 25.833 14.936 1.00 31.11
ATOM 1848 CB ASP 253 1.158 26.854 15.557 1.00 30.03
ATOM 1849 CG ASP 253 0.521 27.751 14.520 1.00 34.22
ATOM 1850 OD1 ASP 253 -0.079 28.786 14.894 1.00 36.89
ATOM 1851 OD2 ASP 253 0.619 27.415 13.320 1.00 37.40
ATOM 1852 C ASP 253 3.528 26.169 15.348 1.00 29.66
ATOM 1853 O ASP 253 4.332 26.690 14.566 1.00 28.81
ATOM 1854 N ALA 254 3.817 25.855 16.603 1.00 26.55
ATOM 1855 CA ALA 254 5.115 26.091 17.184 1.00 25.62
ATOM 1856 CB ALA 254 5.140 25.564 18.598 1.00 20.85
ATOM 1857 C ALA 254 6.196 25.405 16.345 1.00 29.11
ATOM 1858 O ALA 254 7.213 26.018 15.997 1.00 31.34
ATOM 1859 N HIS 255 5.986 24.137 16.013 1.00 27.36
ATOM 1860 CA HIS 255 6.973 23.410 15.231 1.00 26.27
ATOM 1861 CB HIS 255 6.428 22.033 14.880 1.00 24.83
ATOM 1862 CG HIS 255 7.341 21.223 14.017 1.00 24.24
ATOM 1863 CD2 HIS 255 7.154 20.674 12.790 1.00 23.27
ATOM 1864 ND1 HIS 255 8.627 20.897 14.390 1.00 24.00
ATOM 1865 CE1 HIS 255 9.193 20.185 13.431 1.00 21.55
ATOM 1866 NE2 HIS 255 8.322 20.035 12.449 1.00 17.44
ATOM 1867 C HIS 255 7.255 24.204 13.962 1.00 28.52
ATOM 1868 O HIS 255 8.409 24.258 13.494 1.00 28.00
ATOM 1869 N LEU 256 6.199 24.835 13.435 1.00 28.61
ATOM 1870 CA LEU 256 6.265 25.621 12.207 1.00 28.55
ATOM 1871 CB LEU 256 4.896 25.596 11.554 1.00 29.17
ATOM 1872 CG LEU 256 4.695 24.226 10.899 1.00 33.99
ATOM 1873 CD1 LEU 256 3.236 23.981 10.647 1.00 38.97
ATOM 1874 CD2 LEU 256 5.494 24.144 9.599 1.00 35.28
ATOM 1875 C LEU 256 6.749 27.045 12.359 1.00 29.39
ATOM 1876 O LEU 256 6.735 27.824 11.410 1.00 31.07
ATOM 1877 N HIS 257 7.185 27.371 13.567 1.00 29.83
ATOM 1878 CA HIS 257 7.673 28.705 13.884 1.00 29.56
ATOM 1879 CB HIS 257 6.605 29.480 14.656 1.00 32.53
ATOM 1880 CG HIS 257 5.426 29.866 13.824 1.00 33.82
ATOM 1881 CD2 HIS 257 4.196 29.316 13.713 1.00 30.44
ATOM 1882 ND1 HIS 257 5.454 30.929 12.945 1.00 33.02
ATOM 1883 CE1 HIS 257 4.289 31.017 12.329 1.00 29.43
ATOM 1884 NE2 HIS 257 3.508 30.048 12.778 1.00 28.63
ATOM 1885 C HIS 257 8.952 28.740 14.698 1.00 28.51
ATOM 1886 O HIS 257 8.903 28.655 15.927 1.00 29.13
ATOM 1887 N TYR 258 10.085 28.896 14.011 1.00 27.00
ATOM 1888 CA TYR 258 11.407 29.000 14.646 1.00 25.03
ATOM 1889 CB TYR 258 12.267 27.784 14.273 1.00 19.74
ATOM 1890 CG TYR 258 11.874 26.563 15.076 1.00 17.11
ATOM 1891 CD1 TYR 258 10.716 25.851 14.768 1.00 17.80
ATOM 1892 CE1 TYR 258 10.301 24.765 15.565 1.00 19.70
ATOM 1893 CD2 TYR 258 12.617 26.158 16.197 1.00 15.62
ATOM 1894 CE2 TYR 258 12.216 25.074 16.977 1.00 18.52
ATOM 1895 CZ TYR 258 11.060 24.380 16.660 1.00 21.26
ATOM 1896 OH TYR 258 10.683 23.269 17.405 1.00 21.26
ATOM 1897 C TYR 258 12.117 30.332 14.287 1.00 25.34
ATOM 1898 O TYR 258 12.913 30.416 13.329 1.00 23.81
ATOM 1899 N PHE 259 11.800 31.373 15.063 1.00 27.51
ATOM 1900 CA PHE 259 12.347 32.716 14.846 1.00 30.42
ATOM 1901 CB PHE 259 13.842 32.655 14.522 1.00 26.75
ATOM 1902 CG PHE 259 14.682 32.157 15.645 1.00 27.90
ATOM 1903 CD1 PHE 259 14.429 32.559 16.958 1.00 30.40
ATOM 1904 CD2 PHE 259 15.769 31.325 15.401 1.00 29.77
ATOM 1905 CE1 PHE 259 15.247 32.145 18.006 1.00 31.66
ATOM 1906 CE2 PHE 259 16.604 30.904 16.472 1.00 25.94
ATOM 1907 CZ PHE 259 16.335 31.317 17.760 1.00 27.26
ATOM 1908 C PHE 259 11.610 33.315 13.658 1.00 32.76
ATOM 1909 O PHE 259 11.661 34.518 13.400 1.00 35.13
ATOM 1910 N GLN 260 10.913 32.462 12.932 1.00 31.61
ATOM 1911 CA GLN 260 10.214 32.941 11.782 1.00 31.13
ATOM 1912 CB GLN 260 11.229 33.310 10.703 1.00 27.17
ATOM 1913 CG GLN 260 11.710 32.116 9.873 1.00 25.63
ATOM 1914 CD GLN 260 12.849 32.468 8.940 1.00 26.16
ATOM 1915 OE1 GLN 260 13.064 31.793 7.937 1.00 27.34
ATOM 1916 NE2 GLN 260 13.598 33.520 9.275 1.00 31.37
ATOM 1917 C GLN 260 9.352 31.798 11.323 1.00 34.49
ATOM 1918 O GLN 260 9.301 30.750 11.950 1.00 35.38
ATOM 1919 N ALA 261 8.657 32.008 10.218 1.00 36.41
ATOM 1920 CA ALA 261 7.805 30.973 9.660 1.00 37.19
ATOM 1921 CB ALA 261 6.737 31.591 8.766 1.00 34.82
ATOM 1922 C ALA 261 8.657 29.993 8.852 1.00 40.12
ATOM 1923 O ALA 261 9.423 30.386 7.961 1.00 37.41
ATOM 1924 N THR 262 8.526 28.717 9.194 1.00 43.36
ATOM 1925 CA THR 262 9.231 27.613 8.539 1.00 44.18
ATOM 1926 CB THR 262 8.924 26.319 9.285 1.00 43.35
ATOM 1927 OG1 THR 262 9.612 26.303 10.537 1.00 44.23
ATOM 1928 CG2 THR 262 9.289 25.118 8.449 1.00 42.27
ATOM 1929 C THR 262 8.739 27.416 7.097 1.00 46.97
ATOM 1930 O THR 262 9.505 27.391 6.130 1.00 44.95
ATOM 1931 N ASP 263 7.425 27.248 7.013 1.00 49.53
ATOM 1932 CA ASP 263 6.660 27.035 5.798 1.00 48.98
ATOM 1933 CB ASP 263 5.238 26.685 6.223 1.00 47.39
ATOM 1934 CG ASP 263 4.644 27.730 7.170 1.00 50.68
ATOM 1935 OD1 ASP 263 5.279 28.036 8.199 1.00 51.25
ATOM 1936 OD2 ASP 263 3.542 28.255 6.887 1.00 53.12
ATOM 1937 C ASP 263 6.635 28.246 4.847 1.00 49.91
ATOM 1938 O ASP 263 5.878 28.258 3.876 1.00 50.87
ATOM 1939 N ALA 264 7.464 29.253 5.107 1.00 48.36
ATOM 1940 CA ALA 264 7.471 30.450 4.265 1.00 47.66
ATOM 1941 CB ALA 264 8.601 31.406 4.678 1.00 46.98
ATOM 1942 C ALA 264 7.615 30.083 2.797 1.00 48.80
ATOM 1943 O ALA 264 6.810 30.519 1.961 1.00 46.94
ATOM 1944 N CYS 265 8.621 29.261 2.488 1.00 52.02
ATOM 1945 CA CYS 265 8.858 28.862 1.107 1.00 52.57
ATOM 1946 C CYS 265 8.071 27.619 0.660 1.00 53.21
ATOM 1947 O CYS 265 8.410 27.015 -0.352 1.00 52.62
ATOM 1948 CB CYS 265 10.356 28.641 0.894 1.00 51.98
ATOM 1949 SG CYS 265 10.914 29.221 -0.736 1.00 62.77
ATOM 1950 N SER 266 7.016 27.256 1.397 1.00 55.18
ATOM 1951 CA SER 266 6.186 26.076 1.094 1.00 55.25
ATOM 1952 CB SER 266 5.315 26.296 -0.163 1.00 55.01
ATOM 1953 OG SER 266 6.059 26.313 -1.376 1.00 53.51
ATOM 1954 C SER 266 7.013 24.807 0.920 1.00 57.82
ATOM 1955 O SER 266 6.771 23.800 1.592 1.00 58.50
ATOM 1956 N THR 288 -5.104 10.709 9.431 1.00 69.17
ATOM 1957 CA THR 288 -6.163 9.702 9.336 1.00 68.04
ATOM 1958 CB THR 288 -5.575 8.326 8.904 1.00 69.10
ATOM 1959 OG1 THR 288 -4.241 8.190 9.427 1.00 71.00
ATOM 1960 CG2 THR 288 -5.550 8.209 7.381 1.00 66.83
ATOM 1961 C THR 288 -6.897 9.563 10.669 1.00 66.62
ATOM 1962 O THR 288 -7.678 8.635 10.867 1.00 67.87
ATOM 1963 N MET 289 -6.637 10.504 11.573 1.00 63.79
ATOM 1964 CA MET 289 -7.267 10.517 12.887 1.00 59.84
ATOM 1965 CB MET 289 -6.376 9.822 13.911 1.00 60.37
ATOM 1966 CG MET 289 -5.070 10.549 14.185 1.00 58.53
ATOM 1967 SD MET 289 -4.097 9.750 15.488 1.00 55.89
ATOM 1968 CE MET 289 -3.213 8.434 14.513 1.00 53.56
ATOM 1969 C MET 289 -7.504 11.958 13.308 1.00 56.91
ATOM 1970 O MET 289 -6.766 12.851 12.911 1.00 56.23
ATOM 1971 N THR 290 -8.523 12.178 14.128 1.00 55.14
ATOM 1972 CA THR 290 -8.862 13.526 14.563 1.00 55.59
ATOM 1973 CB THR 290 -10.041 13.530 15.540 1.00 55.73
ATOM 1974 OG1 THR 290 -9.587 13.157 16.847 1.00 56.69
ATOM 1975 CG2 THR 290 -11.103 12.551 15.081 1.00 57.65
ATOM 1976 C THR 290 -7.722 14.275 15.228 1.00 55.10
ATOM 1977 O THR 290 -6.549 13.938 15.071 1.00 57.70
ATOM 1978 N ASP 291 -8.086 15.311 15.969 1.00 53.52
ATOM 1979 CA ASP 291 -7.113 16.122 16.666 1.00 53.03
ATOM 1980 CB ASP 291 -7.539 17.584 16.604 1.00 53.76
ATOM 1981 CG ASP 291 -6.579 18.435 15.798 1.00 53.50
ATOM 1982 OD1 ASP 291 -6.217 18.039 14.670 1.00 54.08
ATOM 1983 OD2 ASP 291 -6.186 19.509 16.294 1.00 53.04
ATOM 1984 C ASP 291 -7.032 15.648 18.108 1.00 53.15
ATOM 1985 O ASP 291 -5.974 15.205 18.567 1.00 50.18
ATOM 1986 N ALA 292 -8.164 15.732 18.807 1.00 54.12
ATOM 1987 CA ALA 292 -8.254 15.306 20.201 1.00 53.85
ATOM 1988 CB ALA 292 -9.705 15.131 20.603 1.00 55.19
ATOM 1989 C ALA 292 -7.540 13.988 20.293 1.00 52.42
ATOM 1990 O ALA 292 -6.840 13.687 21.244 1.00 53.42
ATOM 1991 N GLU 293 -7.749 13.198 19.264 1.00 52.24
ATOM 1992 CA GLU 293 -7.121 11.906 19.160 1.00 55.82
ATOM 1993 CB GLU 293 -7.529 11.259 17.837 1.00 59.67
ATOM 1994 CG GLU 293 -6.842 9.964 17.550 1.00 64.61
ATOM 1995 CD GLU 293 -7.295 8.872 18.483 1.00 67.82
ATOM 1996 OE1 GLU 293 -7.070 9.000 19.708 1.00 67.87
ATOM 1997 OE2 GLU 293 -7.883 7.886 17.987 1.00 70.08
ATOM 1998 C GLU 293 -5.602 12.064 19.218 1.00 54.54
ATOM 1999 O GLU 293 -4.964 11.753 20.230 1.00 52.29
ATOM 2000 N LEU 294 -5.034 12.569 18.127 1.00 52.22
ATOM 2001 CA LEU 294 -3.594 12.745 18.036 1.00 49.88
ATOM 2002 CB LEU 294 -3.213 13.548 16.782 1.00 46.75
ATOM 2003 CG LEU 294 -1.734 13.465 16.357 1.00 46.25
ATOM 2004 CD1 LEU 294 -1.411 12.023 15.956 1.00 41.73
ATOM 2005 CD2 LEU 294 -1.450 14.414 15.187 1.00 45.50
ATOM 2006 C LEU 294 -3.012 13.418 19.262 1.00 49.36
ATOM 2007 O LEU 294 -1.936 13.061 19.715 1.00 48.76
ATOM 2008 N GLU 295 -3.714 14.397 19.810 1.00 49.05
ATOM 2009 CA GLU 295 -3.183 15.071 20.981 1.00 47.81
ATOM 2010 CB GLU 295 -4.094 16.204 21.408 1.00 48.38
ATOM 2011 CG GLU 295 -3.765 16.655 22.787 1.00 50.22
ATOM 2012 CD GLU 295 -4.643 17.782 23.219 1.00 52.29
ATOM 2013 OE1 GLU 295 -4.536 18.869 22.617 1.00 54.49
ATOM 2014 OE2 GLU 295 -5.450 17.580 24.154 1.00 52.86
ATOM 2015 C GLU 295 -3.055 14.084 22.126 1.00 46.68
ATOM 2016 O GLU 295 -2.019 14.009 22.801 1.00 45.14
ATOM 2017 N LYS 296 -4.129 13.341 22.352 1.00 45.98
ATOM 2018 CA LYS 296 -4.123 12.365 23.405 1.00 47.29
ATOM 2019 CB LYS 296 -5.454 11.633 23.432 1.00 47.12
ATOM 2020 CG LYS 296 -6.558 12.517 23.981 1.00 47.16
ATOM 2021 CD LYS 296 -7.900 11.809 24.010 1.00 50.91
ATOM 2022 CE LYS 296 -8.985 12.717 24.580 1.00 49.77
ATOM 2023 NZ LYS 296 -10.319 12.055 24.645 1.00 50.40
ATOM 2024 C LYS 296 -2.963 11.411 23.204 1.00 45.67
ATOM 2025 O LYS 296 -2.025 11.405 24.001 1.00 45.50
ATOM 2026 N LYS 297 -3.007 10.620 22.141 1.00 45.96
ATOM 2027 CA LYS 297 -1.927 9.678 21.894 1.00 46.33
ATOM 2028 CB LYS 297 -1.922 9.248 20.447 1.00 45.64
ATOM 2029 CG LYS 297 -3.017 8.264 20.143 1.00 49.76
ATOM 2030 CD LYS 297 -3.095 7.965 18.652 1.00 52.12
ATOM 2031 CE LYS 297 -4.241 7.000 18.307 1.00 53.58
ATOM 2032 NZ LYS 297 -4.433 6.842 16.829 1.00 55.41
ATOM 2033 C LYS 297 -0.578 10.252 22.236 1.00 45.70
ATOM 2034 O LYS 297 0.164 9.692 23.040 1.00 45.62
ATOM 2035 N LEU 298 -0.275 11.392 21.639 1.00 43.91
ATOM 2036 CA LEU 298 0.997 12.035 21.873 1.00 43.35
ATOM 2037 CB LEU 298 1.104 13.280 21.030 1.00 42.24
ATOM 2038 CG LEU 298 1.281 12.939 19.560 1.00 42.32
ATOM 2039 CD1 LEU 298 1.715 14.203 18.864 1.00 40.59
ATOM 2040 CD2 LEU 298 2.330 11.824 19.372 1.00 39.55
ATOM 2041 C LEU 298 1.257 12.381 23.307 1.00 43.14
ATOM 2042 O LEU 298 2.360 12.228 23.806 1.00 43.84
ATOM 2043 N ASN 299 0.231 12.869 23.970 1.00 42.30
ATOM 2044 CA ASN 299 0.369 13.215 25.367 1.00 40.09
ATOM 2045 CB ASN 299 -0.987 13.640 25.937 1.00 39.75
ATOM 2046 CG ASN 299 -1.236 15.126 25.817 1.00 39.45
ATOM 2047 OD1 ASN 299 -0.602 15.841 25.020 1.00 36.52
ATOM 2048 ND2 ASN 299 -2.182 15.604 26.610 1.00 39.54
ATOM 2049 C ASN 299 0.845 11.952 26.061 1.00 39.47
ATOM 2050 O ASN 299 1.896 11.917 26.688 1.00 39.95
ATOM 2051 N SER 300 0.030 10.915 25.909 1.00 36.98
ATOM 2052 CA SER 300 0.255 9.596 26.477 1.00 32.52
ATOM 2053 CB SER 300 -0.477 8.546 25.651 1.00 32.63
ATOM 2054 OG SER 300 0.327 7.392 25.490 1.00 40.74
ATOM 2055 C SER 300 1.725 9.242 26.529 1.00 31.83
ATOM 2056 O SER 300 2.242 8.833 27.582 1.00 26.37
ATOM 2057 N TYR 301 2.382 9.392 25.381 1.00 32.44
ATOM 2058 CA TYR 301 3.793 9.096 25.255 1.00 33.17
ATOM 2059 CB TYR 301 4.245 9.455 23.850 1.00 31.08
ATOM 2060 CG TYR 301 3.738 8.476 22.832 1.00 31.92
ATOM 2061 CD1 TYR 301 3.218 8.903 21.611 1.00 34.78
ATOM 2062 CE1 TYR 301 2.692 7.981 20.684 1.00 35.21
ATOM 2063 CD2 TYR 301 3.737 7.109 23.108 1.00 32.63
ATOM 2064 CE2 TYR 301 3.220 6.183 22.202 1.00 33.64
ATOM 2065 CZ TYR 301 2.693 6.625 20.993 1.00 36.58
ATOM 2066 OH TYR 301 2.122 5.720 20.122 1.00 42.92
ATOM 2067 C TYR 301 4.650 9.814 26.289 1.00 33.19
ATOM 2068 O TYR 301 5.382 9.169 27.062 1.00 33.66
ATOM 2069 N VAL 302 4.551 11.146 26.291 1.00 31.53
ATOM 2070 CA VAL 302 5.309 11.986 27.207 1.00 31.88
ATOM 2071 CB VAL 302 5.184 13.516 26.830 1.00 30.64
ATOM 2072 CG1 VAL 302 3.806 13.804 26.245 1.00 34.62
ATOM 2073 CG2 VAL 302 5.425 14.390 28.033 1.00 28.18
ATOM 2074 C VAL 302 4.872 11.714 28.638 1.00 34.16
ATOM 2075 O VAL 302 5.579 12.060 29.582 1.00 35.35
ATOM 2076 N GLU 303 3.725 11.075 28.822 1.00 37.44
ATOM 2077 CA GLU 303 3.322 10.774 30.182 1.00 38.93
ATOM 2078 CB GLU 303 1.814 10.559 30.249 1.00 41.44
ATOM 2079 CG GLU 303 1.131 11.315 31.410 1.00 48.53
ATOM 2080 CD GLU 303 1.164 12.848 31.252 1.00 53.98
ATOM 2081 OE1 GLU 303 0.499 13.392 30.337 1.00 55.14
ATOM 2082 OE2 GLU 303 1.861 13.520 32.046 1.00 56.20
ATOM 2083 C GLU 303 4.094 9.512 30.572 1.00 38.83
ATOM 2084 O GLU 303 4.418 9.294 31.745 1.00 41.64
ATOM 2085 N MET 304 4.405 8.693 29.570 1.00 37.27
ATOM 2086 CA MET 304 5.155 7.467 29.801 1.00 35.94
ATOM 2087 CB MET 304 4.953 6.478 28.650 1.00 31.52
ATOM 2088 CG MET 304 3.579 5.858 28.595 1.00 31.49
ATOM 2089 SD MET 304 3.184 4.590 29.827 1.00 28.57
ATOM 2090 CE MET 304 2.746 5.557 31.251 1.00 29.07
ATOM 2091 C MET 304 6.632 7.809 29.934 1.00 37.54
ATOM 2092 O MET 304 7.336 7.256 30.778 1.00 37.89
ATOM 2093 N ASP 305 7.099 8.729 29.098 1.00 36.62
ATOM 2094 CA ASP 305 8.494 9.138 29.142 1.00 36.97
ATOM 2095 CB ASP 305 8.789 10.139 28.020 1.00 35.93
ATOM 2096 CG ASP 305 8.797 9.478 26.641 1.00 35.21
ATOM 2097 OD1 ASP 305 9.366 8.364 26.550 1.00 26.08
ATOM 2098 OD2 ASP 305 8.256 10.063 25.663 1.00 35.03
ATOM 2099 C ASP 305 8.830 9.727 30.507 1.00 36.38
ATOM 2100 O ASP 305 9.907 9.521 31.037 1.00 36.84
ATOM 2101 N LYS 306 7.878 10.407 31.112 1.00 34.64
ATOM 2102 CA LYS 306 8.121 10.992 32.416 1.00 35.44
ATOM 2103 CB LYS 306 7.036 12.016 32.739 1.00 34.65
ATOM 2104 CG LYS 306 7.286 13.335 32.039 1.00 35.30
ATOM 2105 CD LYS 306 6.029 14.155 31.857 1.00 42.10
ATOM 2106 CE LYS 306 5.507 14.747 33.147 1.00 45.04
ATOM 2107 NZ LYS 306 4.285 15.551 32.855 1.00 48.24
ATOM 2108 C LYS 306 8.178 9.927 33.488 1.00 35.92
ATOM 2109 O LYS 306 9.026 9.975 34.392 1.00 34.83
ATOM 2110 N GLU 307 7.281 8.955 33.369 1.00 38.05
ATOM 2111 CA GLU 307 7.209 7.871 34.334 1.00 41.32
ATOM 2112 CB GLU 307 6.007 6.986 34.035 1.00 41.96
ATOM 2113 CG GLU 307 5.559 6.166 35.230 1.00 44.27
ATOM 2114 CD GLU 307 4.739 4.938 34.841 1.00 44.72
ATOM 2115 OE1 GLU 307 3.890 5.025 33.925 1.00 42.09
ATOM 2116 OE2 GLU 307 4.938 3.876 35.470 1.00 46.86
ATOM 2117 C GLU 307 8.478 7.042 34.251 1.00 40.66
ATOM 2118 O GLU 307 8.914 6.445 35.227 1.00 39.62
ATOM 2119 N TYR 308 9.040 6.993 33.050 1.00 39.89
ATOM 2120 CA TYR 308 10.271 6.265 32.795 1.00 38.66
ATOM 2121 CB TYR 308 10.647 6.328 31.306 1.00 38.72
ATOM 2122 CG TYR 308 11.998 5.698 30.995 1.00 35.82
ATOM 2123 CD1 TYR 308 12.092 4.412 30.447 1.00 34.01
ATOM 2124 CE1 TYR 308 13.326 3.802 30.244 1.00 33.27
ATOM 2125 CD2 TYR 308 13.186 6.354 31.323 1.00 35.59
ATOM 2126 CE2 TYR 308 14.430 5.744 31.126 1.00 35.52
ATOM 2127 CZ TYR 308 14.494 4.468 30.592 1.00 35.24
ATOM 2128 OH TYR 308 15.722 3.846 30.457 1.00 32.71
ATOM 2129 C TYR 308 11.344 6.968 33.597 1.00 38.16
ATOM 2130 O TYR 308 11.987 6.384 34.466 1.00 42.33
ATOM 2131 N ILE 309 11.546 8.236 33.279 1.00 34.53
ATOM 2132 CA ILE 309 12.539 9.008 33.978 1.00 33.96
ATOM 2133 CB ILE 309 12.485 10.482 33.553 1.00 31.45
ATOM 2134 CG2 ILE 309 12.843 11.392 34.737 1.00 28.90
ATOM 2135 CG1 ILE 309 13.412 10.670 32.339 1.00 30.32
ATOM 2136 CD1 ILE 309 13.436 12.066 31.764 1.00 34.28
ATOM 2137 C ILE 309 12.345 8.877 35.466 1.00 35.18
ATOM 2138 O ILE 309 13.295 8.668 36.212 1.00 33.45
ATOM 2139 N LYS 310 11.103 8.981 35.897 1.00 38.85
ATOM 2140 CA LYS 310 10.825 8.872 37.313 1.00 39.29
ATOM 2141 CB LYS 310 9.323 8.894 37.541 1.00 37.16
ATOM 2142 CG LYS 310 8.964 8.558 38.953 1.00 35.69
ATOM 2143 CD LYS 310 7.641 7.848 38.996 1.00 38.63
ATOM 2144 CE LYS 310 7.527 7.081 40.307 1.00 39.65
ATOM 2145 NZ LYS 310 6.351 6.171 40.381 1.00 44.93
ATOM 2146 C LYS 310 11.403 7.596 37.902 1.00 38.47
ATOM 2147 O LYS 310 12.210 7.610 38.830 1.00 38.54
ATOM 2148 N THR 311 10.972 6.491 37.330 1.00 38.54
ATOM 2149 CA THR 311 11.370 5.180 37.784 1.00 38.10
ATOM 2150 CB THR 311 10.551 4.112 37.056 1.00 37.44
ATOM 2151 OG1 THR 311 10.847 2.832 37.622 1.00 42.06
ATOM 2152 CG2 THR 311 10.868 4.114 35.559 1.00 33.19
ATOM 2153 C THR 311 12.842 4.828 37.666 1.00 36.98
ATOM 2154 O THR 311 13.253 3.757 38.093 1.00 38.27
ATOM 2155 N HIS 312 13.637 5.724 37.102 1.00 36.25
ATOM 2156 CA HIS 312 15.071 5.476 36.940 1.00 36.32
ATOM 2157 CB HIS 312 15.442 5.505 35.455 1.00 36.87
ATOM 2158 CG HIS 312 14.998 4.294 34.693 1.00 42.97
ATOM 2159 CD2 HIS 312 13.776 3.730 34.531 1.00 46.21
ATOM 2160 ND1 HIS 312 15.876 3.515 33.971 1.00 43.74
ATOM 2161 CE1 HIS 312 15.217 2.522 33.396 1.00 45.94
ATOM 2162 NE2 HIS 312 13.939 2.630 33.719 1.00 48.05
ATOM 2163 C HIS 312 15.893 6.520 37.693 1.00 34.17
ATOM 2164 O HIS 312 17.119 6.534 37.654 1.00 29.16
ATOM 2165 N ALA 313 15.195 7.398 38.389 1.00 34.95
ATOM 2166 CA ALA 313 15.841 8.461 39.137 1.00 37.67
ATOM 2167 CB ALA 313 14.808 9.133 40.053 1.00 33.12
ATOM 2168 C ALA 313 17.077 8.046 39.945 1.00 36.45
ATOM 2169 O ALA 313 18.137 8.654 39.811 1.00 35.64
ATOM 2170 N SER 314 16.935 7.006 40.763 1.00 35.30
ATOM 2171 CA SER 314 18.004 6.519 41.636 1.00 34.86
ATOM 2172 CB SER 314 17.387 5.751 42.799 1.00 32.67
ATOM 2173 OG SER 314 16.772 4.571 42.292 1.00 37.73
ATOM 2174 C SER 314 19.021 5.598 40.964 1.00 34.47
ATOM 2175 O SER 314 19.721 4.842 41.648 1.00 35.60
ATOM 2176 N ARG 315 19.110 5.661 39.643 1.00 34.13
ATOM 2177 CA ARG 315 20.026 4.801 38.923 1.00 33.01
ATOM 2178 CB ARG 315 19.315 4.214 37.709 1.00 30.87
ATOM 2179 CG ARG 315 18.160 3.289 38.078 1.00 33.01
ATOM 2180 CD ARG 315 18.596 1.841 38.115 1.00 30.79
ATOM 2181 NE ARG 315 18.950 1.363 36.782 1.00 27.06
ATOM 2182 CZ ARG 315 20.135 0.871 36.456 1.00 23.56
ATOM 2183 NH1 ARG 315 21.079 0.788 37.372 1.00 19.72
ATOM 2184 NH2 ARG 315 20.368 0.476 35.214 1.00 19.00
ATOM 2185 C ARG 315 21.316 5.486 38.508 1.00 36.88
ATOM 2186 O ARG 315 21.687 6.530 39.056 1.00 33.34
ATOM 2187 N SER 316 21.992 4.873 37.533 1.00 42.86
ATOM 2188 CA SER 316 23.281 5.337 36.997 1.00 44.60
ATOM 2189 CB SER 316 23.248 6.850 36.667 1.00 45.66
ATOM 2190 OG SER 316 24.311 7.227 35.802 1.00 45.23
ATOM 2191 C SER 316 24.329 5.034 38.070 1.00 44.35
ATOM 2192 O SER 316 25.091 4.052 37.885 1.00 46.99
ATOM 2193 OXT SER 316 24.345 5.751 39.093 1.00 40.99
Claims (29)
1.一种具有磷脂酶抑制活性的分离的肽,选自:
(a)分离的肽,其包含与SEQ ID NO:1的289-310残基或SEQ ID NO:10的154-175残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的氨基酸序列;
(b)由多核苷酸编码的分离的肽,所述多核苷酸在中等严格条件或高严格条件下与SEQ ID NO:1的肽编码序列或所述SEQ ID NO:1的肽编码序列的互补链杂交;
(c)由多核苷酸编码的分离的肽,所述多核苷酸包含与SEQ ID NO:1的289-310残基具有至少65%、至少70%、至少75%、至少80%、至少85%、至少90%、至少95%、至少97%或100%同一性的核苷酸序列;或
(d)分离的肽,其包含具有下列氨基酸序列的基序:M1T2D3X4X5L6E7X8K9L10N11X12Y13V14X15X16D17X18E19Y20X21K22,其中X4、X5、X8、X12、X15、X16、X18和X21独立地可为任何氨基酸,
其中该肽的大小小于60个氨基酸(aa)。
2.权利要求1的肽,其具有少于55aa,少于50aa,少于45aa,少于40aa,少于35aa,或少于30aa的长度。
3.权利要求1或2的肽,其具有至少15aa,至少20aa或至少25aa的长度。
4.权利要求1的肽,其中所述肽具有α-螺旋的二级结构。
5.权利要求1的肽,其中在基序中存在的X4、X5、X8、X12、X15、X16、X18和X21位置中每一个的氨基酸独立地选取,使得X4为A或E;X5为E或Q;X8为K或A;X12为S或N;X15为E、Q或A;X16为L或M;X18为K或Q;而X21为I或V。
6.权利要求1-4任一项所述的肽,其与SEQ ID NO:1相比在对应于FoL残基D291;L294;E295;K297;L298;N299;Y301;D305;K306;Y308;或V309的位置处包含至少一个氨基酸取代。
7.权利要求5所述的肽,其与SEQ ID NO:1相比在对应于FoL残基D291E;L294A;E295T,S;K297R;L298A;N299D,E;Y301W;D305A;K306R;Y308E,D;或V309I,N,Q处包含至少一个氨基酸取代。
8.编码权利要求1-7任一项所述的肽的分离多核苷酸。
9.一种核酸构建体,其包含权利要求8的多核苷酸,所述多核苷酸可操作连接于至少一个控制序列,其在表达宿主中引导所述肽的产生。
10.包含权利要求9的核酸构建体的重组表达载体。
11.包含权利要求9的核酸构建体或权利要求10的重组表达载体的重组宿主细胞。
12.一种制备权利要求1-7任一项所述的分离的肽的方法,包括下列步骤:
(a)在有益于所述肽产生的条件下培养包含所述核酸构建体的宿主细胞,所述核酸构建体包含编码所述肽至少一个拷贝的多核苷酸;和
(b)回收所述肽。
13.权利要求1-7任一项所述的肽在将所述肽结合于脂肪酶时抑制所述脂肪酶酶活性的用途。
14.包含权利要求1-7任一项所述的肽和脂肪酶的多肽,其中所述脂肪酶在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性,和/或不显示磷脂酶活性。
15.权利要求14的包含脂肪酶的多肽,所述脂肪酶与细毛嗜热霉(Thermomyces lanuginosus)(SEQ ID NO:9)具有至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%的同一性。
16.权利要求14-15任一项所述的多肽,其中所述脂肪酶包含至少一个改变,其独立地为插入、缺失或取代。
17.权利要求16的多肽,其中所述脂肪酶的至少一个改变包含对应于细毛嗜热霉(SEQ ID NO:9)的残基E87R、I90L、G91T、I202P、R209L、E210I或T244L的一个或多个氨基酸取代。
18.权利要求15的多肽,其中所述脂肪酶包含用尖镰孢(Fusarium oxysporum)(SEQ ID NO:1)的残基246-254取代对应细毛嗜热霉(SEQ ID NO:9)的248-255的残基,或用尖镰孢(SEQ ID NO:1)的残基247-252取代细毛嗜热霉(SEQ ID NO:9)的248-253。
19.一种具有磷脂酶抑制活性的多肽,其中具有α-螺旋的至少三个溶剂可接近的残基位于其表面的蛋白质在该α-螺旋中对应于位置D3、L6、L10、Y13、V14、D17和X21的至少一个所述溶剂可接近的残基处和/或对应于权利要求1的基序的位置E7、K9、N11、X18和Y20的边缘残基处经修饰。
20.权利要求19的多肽,其中所述亲本蛋白质具有与菌丝霉素(SEQ ID NO:11);莫尼糖蛋白(SEQ ID NO:12);Protegrin(SEQ ID NO:13);芽孢杆菌RNA酶(Barnase)(SEQ ID NO:14);胱蛋白(SEQ ID NO:15);或载脂蛋白E(SEQ ID NO:16)具有至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%同一性的氨基酸序列。
21.制备权利要求14-18任一项所述的多肽的方法,包括将权利要求1-7任一项所述的肽附加于脂肪酶的步骤,其中所述脂肪酶具有在平板测定法中低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性,和/或不显示任何磷脂酶活性。
22.一种制备多肽的方法,包括下述步骤:
(a)选择在蛋白质表面具有α-螺旋的蛋白质,由此所述α-螺旋的至少三个,至少四个,至少五个或至少六个残基是溶剂可接近的;
(b)将所述蛋白质的α-螺旋与权利要求1-7任一项所述的肽进行比对;
(c)鉴定出所述蛋白质的α-螺旋上溶剂可接近的残基,其对应于权利要求1的所述基序的位置D3、L6、L10、Y13、V14、D17和X21;
(d)在步骤(c)中鉴定出的蛋白质中改变至少一个,至少两个,至少三个,至少四个,至少五个,至少六个或至少七个氨基酸;
(e)测试所述多肽的磷脂酶抑制活性;
(f)选择具有磷脂酶抑制活性的多肽;和
(g)产生(f)中选出的多肽。
23.权利要求22的方法,其还在步骤(c)中鉴定所述蛋白质的α-螺旋中的残基,其潜在地为溶剂可接近的,对应于权利要求1的所述基序的位置E7、K9、N11、X18和Y20。
24.权利要求23的方法,其还包括在所述蛋白质中一个或多个位置进行至少一个改变的步骤。
25.权利要求14-20任一项所述的多肽在将包含于所述多肽中的肽结合于脂肪酶时抑制所述脂肪酶的酶活性的用途。
26.一种脂肪酶,其在平板测定法中具有低于50PHLU/mg,低于45PHLU/mg,低于40PHLU/mg,低于35PHLU/mg,低于30PHLU/mg,低于25PHLU/mg,低于20PHLU/mg,低于15PHLU/mg,低于10PHLU/mg,低于5PHLU/mg或低于1PHLU/mg的磷脂酶活性,和/或不显示任何磷脂酶活性,其能够受权利要求1-7任一项所述的肽抑制,其中所述脂肪酶包含至少一个改变,其独立地为插入、缺失或取代,由此所述脂肪酶的活性在结合(a)权利要求1-8任一项所述的具有磷脂酶抑制活性的分离的肽;或(b)权利要求15-21任一项所述的包含于所述多肽中具有磷脂酶抑制活性的肽至少之一时受抑制。
27.权利要求26的脂肪酶,其中所述脂肪酶与细毛嗜热霉(SEQ ID NO:9)具有至少70%,至少75%,至少80%,至少85%,至少90%,至少95%,至少97%或100%的同一性。
28.权利要求27的脂肪酶,其中至少一个改变是对应于细毛嗜热霉(SEQ ID NO:9)的残基L92;R96;L203;I207;R211;L243;L250;或L252的取代。
29.权利要求28的脂肪酶,其中所述至少一个改变是对应于细毛嗜热霉(SEQ ID NO:9)的残基L92D,E,W;R96E,D,A;L203W,K,M;I207D,E;R211H;L243W,K L250D,E,R和L252S,T的取代。
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CN110914292A (zh) * | 2017-05-12 | 2020-03-24 | 巴斯夫欧洲公司 | 使用脂肪酶用于清洁的方法 |
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AU2010264116B2 (en) * | 2009-06-25 | 2013-12-19 | Dupont Nutrition Biosciences Aps | Protein |
JP6442399B2 (ja) * | 2013-03-28 | 2018-12-19 | 株式会社カネカ | 改変型カルボニル還元酵素およびその遺伝子 |
CN111500552A (zh) | 2014-03-12 | 2020-08-07 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
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CN107245470A (zh) * | 2017-05-24 | 2017-10-13 | 华南理工大学 | 一种脂肪酶重组大肠杆菌表达菌株、重组脂肪酶和应用 |
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