CN102010838B - Bacterial strain for degrading zearalenone toxin and application thereof - Google Patents
Bacterial strain for degrading zearalenone toxin and application thereof Download PDFInfo
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- CN102010838B CN102010838B CN2010102664899A CN201010266489A CN102010838B CN 102010838 B CN102010838 B CN 102010838B CN 2010102664899 A CN2010102664899 A CN 2010102664899A CN 201010266489 A CN201010266489 A CN 201010266489A CN 102010838 B CN102010838 B CN 102010838B
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Abstract
The invention relates to a bacterial strain for degrading zearalenone toxin, which is characterized in that: the preserving number of the bacterial strain is CCTCC NO: M2010053; and the Genbank accession number of the bacterial strain 16S rDNA is HM372880. When in application, the bacterial strain is prepared into liquid bactericide or solid bactericide which can degrade the zearalenone toxin fordegrading the zearalenone toxin in grains or fodders. The bacterial strain has the advantages that: the process of using the bacterial strain to degrade the zearalenone toxin is free of secondary pollution, thereby improving the quality of agricultural products and ensuring the edible safety of people and livestock; and the bacterial strain is used for producing various preparations which are used for degrading the zearalenone toxin the bacterial strain, which is not only low in production and using cost, but also safe in use.
Description
Technical field
The present invention relates to a kind of bacterial strain and application thereof of ability degrading zearalenone, especially a kind of bacterial strain and application thereof to the degraded of zearalenone toxin.
Background technology
Zearalenone (Zearalenone; ZEA) be a kind estrogen-like mycotoxins that produces by sickle-like bacteria; Its chemistry 6-(10-hydroxyl-6-oxygen base-undecenyl) β-Lei by name locks acid lactone, extensively is present in cereal crop such as the corn that goes mouldy, Chinese sorghum, wheat, oat, barley and the milk preparation.Producing the most common Pseudomonas of zearalenone is Fusarium graminearum, is Fusarfum tricinctum, Fusarium equiseti, Fusarium nivale, Fusarlum roseum etc. in addition.Research shows that zearalenone has very strong genotoxicity, can reduce the survival rate of embryo of pregnant animal and the birth of newborn fetus and weigh, and causes the production performance of animal to descend; And this toxin, liver and liver cell are had the intensive toxic action, have immunosuppression, the incidence and development of related neoplasms is played a driving role.
Because used cereal (like corn, wheat) is the best matrix that ZEA produces in feed and the foodstuff production; So cereal all might receive the pollution of ZEA in field, results process, results back storage period and feed and many links such as finished food product storage, use.Wang Ruojun (2003) shows that to national feedstuff raw material and mixed feed detected result the ZEA recall rate reaches 100% in the corn, content range 4.40～368.13 μ g/kg, average 104.99 μ g/kg; Recall rate reaches 92.9% in the protein feed, content range 0～476.0 μ g/kg, average 110.0 μ g/kg; Recall rate reaches 100% in the mixed feed, content range 39.22～230.30 μ g/kg, average 83.96 μ g/kg.
Since zearalenone toxin ubiquity in cereal, and have very big hazardness, therefore find a kind of suitable toxic method of this toxin of removing or reduce to seem particularly important.The treatment method for detoxication that at present the ZEA toxin is adopted is a lot; Comprise liming infusion method, chlorination, decortication method, take off embryo detoxification method, method of chemical treatment, use sorbent material, take off mould dose etc.; Though these all processing all alleviate endotoxin contamination to a certain extent, its maximum deficiency is: the detoxification effect is limited, possibly cause the losing of important nutrient, cost is more high.Therefore this field relevant person thinks that the best approach of detoxification is a biological detoxication, promptly under mild conditions, need not deleterious pharmaceutical chemicals, and can not cause losing of nutritive value, can not reduce palatability yet, and make zearalenone detoxicated.Because microbe population is big, kind is many, genetic resources is abundant, and the potentiality of degradable organic pollutant are very big, and nearly all organism of environmental pollution that causes can both be decomposed by Institute of Micro-biology, and clean thorough, non-secondary pollution.Therefore, the mikrobe detoxification is a best approach of eliminating the zearalenone endotoxin contamination at present.
China is the repeating transmission district of a Plant diseases; And at the airing of agricultural-food and equipment aspect the deposit and technology or relatively backward; This just causes the content of toxins in the agricultural products in China higher, and the existence of toxin is except the yield and quality of serious harm grain, to people and animals' health; Cause beyond the serious threat, also limiting the export trade of agricultural products in China.Also there is not a practicable method for the processing of toxin is domestic, plain products of some detoxifications on the market: as: natural mold toxin sorbent, mikrobe detoxicants etc. are also from external import, and the high price of imported product has hindered the widespread use of these products.
In sum, for the food safety that ensures people and healthy, and guarantee the smooth outlet of agricultural-food; Be necessary the bacterial strain of separation screening efficient degradation zearalenone, study its biological characteristics, degradation characteristic and the detoxifcation mechanism of zearalenone; And combine degradation bacteria strains and suitable material; Select suitable formulation, process the mould detoxicant, further study the detoxification effect of mycotoxin detoxicant as fodder additives and the antibacterial detoxicant of plant.Final for solving the plain pollution problem of forage poisoning, the service efficiency of raising grain guarantees the safety in production of livestock industry, and grain yield and food quality safety control are played very big pushing effect.
Summary of the invention
The objective of the invention is to: to the extensive pollution problems of zearalenone toxin in grain-production and feed processing and the preservation process, developing out can be to the bacterial strain and the application thereof of zearalenone degraded.
The objective of the invention is to realize like this: a strain is to the bacterial strain of zearalenone toxin degraded; It is characterized in that: the preserving number of this bacterial strain is CCTCC NO:M2010053; Its biological characteristics be bacterial strain on nutrition nutrient agar flat board, 30 ℃, cultivate 24h: thalline is shaft-like; 0.68 μ m * 2.13～3.22 μ m, Gram-positive; Gemma is oval, middle life, and sporangiocyst does not expand; Cultivate 48h: the bacterium colony oyster white, drying, irregular, flat, opaque, the edge is irregular; The carbon source that can utilize has: glycerine, L-arabinose, ribose, D-wood sugar, glucose, fructose, seminose, inositol, N.F,USP MANNITOL, sorbyl alcohol, Alpha-Methyl-D-glucoside, VitaminB17, polychrom, salicin, cellobiose, SANMALT-S, lactose, melibiose, sucrose, trehalose, raffinose, starch, glycogen, unavailable carbon source has: glycerine, red tinea alcohol, D-pectinose, L-wood sugar, ribitol, Beta-methyl-D-xyloside, semi-lactosi, sorbose, rhamnosyl, melampyrin, Alpha-Methyl-D-mannoside, N-acetyl-glycosamine, ursin, synanthrin, melizitose, Xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D-Fucose, L-Fucose, D-arabitol, L-arabinose alcohol, gluconate, 2-ketone group-gluconate, 5-ketone group-gluconate.The Genbank number of landing of this bacterial strain 16S rDNA is HM372880.
A kind of above-mentioned can the application of the bacterial strain of zearalenone toxin degraded being is characterized in that:
A) with the activation on petridish of bacterial strain original seed, and measure degradation property, be inoculated in that to obtain the test tube kind on the test tube slant subsequent use;
B) with the test tube kind place contain seed culture medium shake the bottle shaking culture to logarithmic phase, obtain bacterial classification;
C) above-mentioned cultured bacterial classification inoculation is gone into to contain the seeding tank of seed culture medium, be cultured to logarithmic phase, obtain seed liquor;
D) seed liquor that obtains is inoculated into the production jar fermentation culture that contains fermention medium, going out jar formation can be to the liquid bacterial agent of zearalenone toxin degraded.
In the application of the bacterial strain that can degrade to the zearalenone toxin: the nutrient broth medium that described seed culture medium is made up of according to volume ratio following component: peptone 0.5%, Carnis Bovis seu Bubali cream 3%, NaCl 0.5%, and the pH of seed culture medium is 7.0～7.2; Described fermention medium is made up of according to volume ratio following component: glucose 1.0%, (NH
4)
2SO
40.1%, K
2HPO
40.2%, MgSO
40.02%, NaCl 0.01%, CaCO
30.3%, peptone 0.05%, zero(ppm) water is an amount of; The pH of fermention medium is 7.2～7.5.
In the application of the bacterial strain that can degrade to the zearalenone toxin: described seed culture medium needs 121 ℃ of high pressure moist heat sterilization postcooling to 30～35 ℃ of uses; Described bacterial classification is inoculated into seeding tank by 10% inoculum size of seed culture medium in the seeding tank; Described seed liquor is inoculated into the production jar by 10% inoculum size of producing fermention medium in the jar.
In can the application to the bacterial strain of zearalenone toxin degraded: the air flow of sterile air be 1: 0.6～1.2 in the seed culture of seeding tank and the fermentation culture process of producing jar; Stirring velocity is 180～240 rev/mins; Culture temperature is 30～35 ℃; Their incubation time was respectively 48～60 hours, obtained liquid bacterial agent after the fermentation ends, and thalline quantity reaches 10 at least in the liquid bacterial agent
9Individual/ml.
In can application to the bacterial strain of zearalenone toxin degraded: spray in cereal after with described liquid bacterial agent dilution, the zearalenone toxin in the degraded cereal, thalline quantity reaches 10 at least in the liquid bacterial agent after the dilution
7Individual/ml.
In the application of the bacterial strain that can degrade to the zearalenone toxin: with described liquid bacterial agent and the weight ratio absorption of adopting peat or clay absorbent by 1: 4, formation solid fungicide.
In the application of the bacterial strain that can degrade to the zearalenone toxin: described sorbent material is peat or clay.
In the application of the bacterial strain that can degrade to the zearalenone toxin: in cereal, add the solid fungicide uniform mixing, the zearalenone toxin in the degraded cereal according to weight ratio 5%; Or in feed, add the solid fungicide uniform mixing, the zearalenone toxin in the degraded feed according to weight ratio 5%.
In the application of the bacterial strain that can degrade to the zearalenone toxin: described cereal is corn, or wheat, or barley, or paddy, or Chinese sorghum, or millet.
The invention has the advantages that: utilize the strains for degrading zearalenone toxin of preserving number, in the process that solves endotoxin contamination, do not have secondary pollution, thereby improve the quality of agricultural-food, guarantee people and animals' edible safety for CCTCC NO:M2010053; Use the various preparations of preserving number as the bacterial strain production degrading zearalenone toxin of CCTCC NO:M2010053, it is low to have the production use cost, safe in utilization.
Description of drawings
Fig. 1 is ZDS-1 liquid bacterial agent design sketch to the degraded of zearalenone toxin in containing the liquid nutrient medium of toxin;
Fig. 2 is ZDS-1 solid preparation design sketch to the degraded of zearalenone toxin in solid feed.
Embodiment
The acquisition of Bacillus sp.ZDS-1
In the corn field of Jiangsu Province Agriculture Science Institute, gather soil sample, in the laboratory with the zearalenone toxin enrichment culture (30 ℃ go down to posterity once weekly) that repeatedly goes down to posterity; Obtain can the degrading zearalenone toxin bacteria suspension, on the LB substratum, be coated with cultivation (30 ℃, 3 days) to bacteria suspension; The different bacterium colony of picking on flat board; Verify degradation effect one by one to the zearalenone toxin, the bacterial strain that final acquisition can the degrading zearalenone toxin, and it is defined as Bacillus sp.ZDS-1; Add glycerine, preserve at-70 ℃ of refrigerators.
Through measuring the physio-biochemical characteristics of Bacillus sp.ZDS-1; The 16SrDNA sequence of clone Bacillus sp.ZDS-1 also checks order to this 16SrDNA sequence; Carry out BLAST to sequencing result relatively at Genbank, confirmed the phylogenetic evolution status of Bacillus sp.ZDS-1.Qualification results such as physiological and biochemical property result through Bacillus sp.ZDS-1 and phylogenetic evolution status; Finally be accredited as bacillus amyloliquefaciens (Bacillus amyloliquefaciens) (being designated hereinafter simply as ZDS-1) to Bacillus sp.ZDS-1; And in June, 2010 we log on Genbank with the 16S rDNA of ZDS-1 first, the number of landing is HM372880.
2010.03.13 we are deposited at Chinese typical culture collection center, Chinese Wuhan with Bacillus sp.ZDS-1; Culture presevation number is CCTCC NO:M2010053; Main biological characteristics be bacterial strain on nutrition nutrient agar flat board, 30 ℃, cultivate 24h: thalline is shaft-like; 0.68 μ m * 2.13～3.22 μ m, Gram-positive; Gemma is oval, middle life, and sporangiocyst does not expand.Cultivate 48h: the bacterium colony oyster white, drying, irregular, flat, opaque, the edge is irregular.The carbon source that can utilize has: glycerine, L-arabinose, ribose, D-wood sugar, glucose, fructose, seminose, inositol, N.F,USP MANNITOL, sorbyl alcohol, Alpha-Methyl-D-glucoside, VitaminB17, polychrom, salicin, cellobiose, SANMALT-S, lactose, melibiose, sucrose, trehalose, raffinose, starch, glycogen, unavailable carbon source has: glycerine, red tinea alcohol, D-pectinose, L-wood sugar, ribitol, Beta-methyl-D-xyloside, semi-lactosi, sorbose, rhamnosyl, melampyrin, Alpha-Methyl-D-mannoside, N-acetyl-glycosamine, ursin, synanthrin, melizitose, Xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D-Fucose, L-Fucose, D-arabitol, L-arabinose alcohol, gluconate, 2-ketone group-gluconate, 5-ketone group-gluconate.
Embodiment 2
The preparation of liquid bacterial agent
Seed culture medium (nutrient broth medium) is: peptone 0.5%, and Carnis Bovis seu Bubali cream 3%, NaCl 0.5%, and the pH of seed culture medium is 7.0～7.2.
Fermention medium is: glucose 1.0%, (NH
4)
2SO
40.1%, K
2HPO
40.2%, MgSO
40.02%, NaCl0.01%, CaCO
30.3%, peptone 0.05%, zero(ppm) water is an amount of; The pH of fermention medium is 7.2～7.5.
With the activation on seed culture medium of ZDS-1 bacterial strain, and measure degradation property, be inoculated in that to obtain the test tube kind on the test tube slant subsequent use.
The test tube kind is inoculated in the 1000mL that contains the 200mL seed culture medium shakes in the bottle, constant-temperature shaking culture obtains bacterial classification to logarithmic phase.
Adopt 500 liters seeding tank, 400 liters of seed culture medium charging capacitys, the back 121 ℃ of high pressure moist heat sterilizations that finish feed intake; After being cooled to 33 ℃, above-mentioned cultured bacterial classification is inoculated into seeding tank by 10% inoculum size of seed culture medium in the seeding tank, be cultured to logarithmic phase; Obtain seed liquor; During this time: stirring velocity is 220 rev/mins, and sterile air feeding amount is 1: 0.8, about 48～60 hours of incubation time.
Producing tankage is 5 tons, 4.0 tons of fermention medium charging capacitys.Production jar 1.1kg/cm after feeding intake
2Pressure under, 121 ℃ of high pressure moist heat sterilizations are below the sterilization postcooling to 35 ℃; Seed liquor is inoculated into production jar fermentation culture by 10% inoculum size of producing fermention medium in the jar; After the fermentation ends thalline quantity reach 1,000,000,000/more than the ml, the air flow of sterile air is 1 in the culturing process of producing jar: 0.8-1.0, stirring velocity is 240 rev/mins; About 48～60 hours of incubation time, going out jar back formation can be to the liquid bacterial agent of zearalenone toxin degraded.
Embodiment 3
The preparation 1 of solid fungicide
The ZDS-1 liquid bacterial agent that embodiment 2 is produced with gather the drying in the sun peat that sieves that comes and mix the back according to 1: 4 weight ratio and form solid-like, thereby process the solid fungicide of ZDS-1.
Embodiment 4
The preparation 2 of solid fungicide
The clay of buying on the liquid bacterial agent that embodiment 2 is obtained and the market mixes formation solid-like afterwards according to 1: 5 weight ratio, thereby processes the solid fungicide of ZDS-1.
Embodiment 5
The ZDS-1 liquid bacterial agent is to the degradation effect of zearalenone toxin
Containing zearalenone toxin substratum is: (NH
4)
2SO
40.5g/L, KH
2PO
40.5g/L, K
2HPO
41.5g/L, MgSO
40.02g/L, NaCl 0.5g/L, zero(ppm) water is an amount of; The pH value 7.0 of substratum.The zearalenone content of toxins that in substratum, adds respectively is 10mg/L (buying from Sigma company).
The substratum that will contain the zearalenone toxin is divided into control group and test group is carried out parallel test, and the liquid bacterial agent that embodiment 2 is obtained inserts in the test group according to 1% inoculum size and contains in the substratum of toxin, and control group inserts the 1% aseptic fermention medium that provided by embodiment 2; Control group and test group all place 30 ℃; 180r/min, shaking table cultivate sampling respectively after 48 hours, add the methyl alcohol mixing in 1: 1 ratio; After crossing the special-purpose filter of 0.22 μ m liquid chromatography; Carry out performance liquid chromatography and detect, still there are a large amount of zearalenone toxin in detected result such as Fig. 1 in the liquid nutrient medium of control group; And detected in the liquid nutrient medium of test group less than the zearalenone toxin, show through the interaction energy of the ZDS-1 zearalenone toxin in the degradation liquid substratum well.
Embodiment 6
The ZDS-1 solid preparation is to the degradation effect of zearalenone toxin in the feed
The feed that ZEA is infected is divided into control group and test group, and the solid fungicide that embodiment 4 is processed joins in the test group feed mixing according to 5% weight ratio; Control group and test group are all added zero(ppm) water in 1: 1 ratio, and 30 ℃ of effects accurately took by weighing 5g feed sample in triangular flask after 2 hours; Add 30mL methyl alcohol, behind the 50g/L NaCl solution 20mL in shaking table (180r/min) fully mixing 5min, supersound extraction 30min then; Be filtered with filter paper in the separating funnel, add methylene dichloride (dividing each 20mL 3 times) extraction; Dichloromethane layer (lower floor) is through anhydrous sodium sulfate dehydration and be collected in the ground triangular flask, behind the Rotary Evaporators evaporate to dryness, with 4mL dissolve with methanol residue; Promptly get the crude extract of zearalenone; Cross from filling out silicagel column and carry out purifying, elutriant through nitrogen blow concentrate the back and cross the special-purpose filter of 0.22 μ m liquid chromatography after, carry out the performance liquid chromatography detection.The result sees Fig. 2: still have a large amount of zearalenone toxin in the feed of control group; And in the feed of test group, the zearalenone content of toxins obviously reduces, and is visible; The ZDS-1 solid fungicide zearalenone toxin in the feed of degrading well, degradation rate can reach 70%.
Embodiment 7
ZDS-1 liquid bacterial agent dilution back is to the degradation effect of zearalenone toxin in the cereal.
100 times of the ZDS-1 liquid bacterial agent water solution dilutions that embodiment 2 obtains is subsequent use, and in the liquid bacterial agent after the dilution, thalline quantity is 10
7Individual/ml.
The corn dividing that is infected by ZEA is become control group and test group, the ratio of the ZDS-1 liquid bacterial agent after diluting 100 times by weight 1: 1 is sprayed onto in the corn in the test group, control group sprays the centrifugal water that goes that does not contain toxin by weight 1: 1 ratio; Control group and test group place 30 ℃ of effects after 2 hours, accurately take by weighing the 5g sample in triangular flask from control group and test group respectively, add 30mL methyl alcohol; Behind the 50g/L NaCl solution 20mL in the abundant mixing 5min of shaking table (180r/min); Supersound extraction 30min is filtered with filter paper in the separating funnel then, adds methylene dichloride and (divides 3 times; Each 20mL) extraction; Dichloromethane layer (lower floor) is through anhydrous sodium sulfate dehydration and be collected in the ground triangular flask, behind the Rotary Evaporators evaporate to dryness, with 4mL dissolve with methanol residue; Promptly get the crude extract of zearalenone; Cross from filling out silicagel column and carry out purifying, elutriant through nitrogen blow concentrate the back and cross the special-purpose filter of 0.22 μ m liquid chromatography after, with the content of zearalenone toxin in the performance liquid chromatography detection corn.The result sees table 1:
Table 1
| The result | Zearalenol content (mg/L) | Degradation rate (%) |
| Control group | 7.89 | - |
| Experimental group | 1.76 | 77.69 |
More than each embodiment be not to concrete restriction of the present invention, as long as,, utilize the zearalenone toxin in said strains for degrading cereal or the feed, all should belong to protection category of the present invention in conjunction with the basic general knowledge of this area according to the description of claim.
Claims (10)
- One strain can degrading zearalenone the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) of toxin; It is characterized in that: the preserving number of this bacterial strain is CCTCC NO:M2010053; Its biological characteristics be bacterial strain on nutrition nutrient agar flat board, 30 ℃, cultivate 24h: thalline is shaft-like; 0.68 μ m * 2.13～3.22 μ m, Gram-positive; Gemma is oval, middle life, and sporangiocyst does not expand; Cultivate 48h: the bacterium colony oyster white, drying, irregular, flat, opaque, the edge is irregular; The carbon source that can utilize has: glycerine, L-arabinose, ribose, D-wood sugar, glucose, fructose, seminose, inositol, N.F,USP MANNITOL, sorbyl alcohol, Alpha-Methyl-D-glucoside, VitaminB17, polychrom, salicin, cellobiose, SANMALT-S, lactose, melibiose, sucrose, trehalose, raffinose, starch; Unavailable carbon source has: red tinea alcohol, D-pectinose, L-wood sugar, ribitol, Beta-methyl-D-xyloside, semi-lactosi, sorbose, rhamnosyl, melampyrin, Alpha-Methyl-D-mannoside, N-acetyl-glycosamine, ursin, synanthrin, melizitose, Xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D-Fucose, L-Fucose, D-arabitol, L-arabinose alcohol, gluconate; The Genbank number of landing of this bacterial strain 16S rDNA is HM372880.
- 2. the application of the bacillus amyloliquefaciens of an ability degrading zearalenone toxin as claimed in claim 1 is characterized in that:A) with the activation on petridish of bacterial strain original seed, and measure degradation property, be inoculated in that to obtain the test tube kind on the test tube slant subsequent use;B) with the test tube kind place contain seed culture medium shake the bottle shaking culture to logarithmic phase, obtain bacterial classification;C) above-mentioned cultured bacterial classification inoculation is gone into to contain the seeding tank of seed culture medium, be cultured to logarithmic phase, obtain seed liquor;D) seed liquor that obtains is inoculated into the production jar fermentation culture that contains fermention medium, going out jar formation can be to the liquid bacterial agent of zearalenone toxin degraded.
- 3. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 2; It is characterized in that: the nutrient broth medium of described seed culture medium for forming: peptone 0.5% by following component; Carnis Bovis seu Bubali cream 3%; NaCl 0.5%, and the pH of seed culture medium is 7.0～7.2; Described fermention medium is to be made up of following component: glucose 1.0%, (NH 4) 2SO 40.1%, K 2HPO 40.2%, MgSO 40.02%, NaCl 0.01%, CaCO 30.3%, peptone 0.05%, zero(ppm) water is an amount of; The pH of fermention medium is 7.2～7.5.
- 4. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 3 is characterized in that: 121 ℃ of high pressure moist heat sterilizations of described seed culture medium needs postcooling to 30～35 ℃ of uses; Described bacterial classification is inoculated into seeding tank by 10% inoculum size of seed culture medium in the seeding tank; Described seed liquor is inoculated into the production jar by 10% inoculum size of producing fermention medium in the jar.
- According to claim 2 or 3 or 4 described can degrading zearalenone the application of bacillus amyloliquefaciens of toxin; It is characterized in that: the air flow of sterile air is 1: 0.6～1.2 in the seed culture of seeding tank and the fermentation culture process of producing jar; Stirring velocity is 180～240 rev/mins, and culture temperature is 30～35 ℃, and incubation time is 48～60 hours; Obtain liquid bacterial agent after the fermentation ends, thalline quantity reaches 10 at least in the liquid bacterial agent 9Individual/ml.
- 6. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 5 is characterized in that: with spraying application in the cereal zearalenone toxin in the degraded cereal after the described liquid bacterial agent dilution; Thalline quantity reaches 10 at least in the liquid bacterial agent after the dilution 7Individual/ml.
- 7. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 5; It is characterized in that: described liquid bacterial agent and sorbent material are adsorbed by 1: 4 weight ratio; Form solid fungicide, wherein said sorbent material is peat or clay.
- 8. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 7 is characterized in that: in cereal, add the solid fungicide uniform mixing according to weight ratio 5%, the zearalenone toxin in the degraded cereal; Or in feed, add the solid fungicide uniform mixing, the zearalenone toxin in the degraded feed according to weight ratio 5%.
- 9. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 6, it is characterized in that: described cereal is corn, or wheat, or barley, or paddy, or Chinese sorghum, or millet.
- 10. the application of the bacillus amyloliquefaciens of ability degrading zearalenone toxin according to claim 8, it is characterized in that: described cereal is corn, or wheat, or barley, or paddy, or jowar, or millet.
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| CN102550893B (en) * | 2011-12-31 | 2013-03-20 | 青岛蔚蓝生物集团有限公司 | Micro-biological degradation method for zearalenone |
| CN103627651A (en) * | 2013-10-08 | 2014-03-12 | 暨南大学 | Bacillus thuringiensis for decomposing zearalenone and application thereof |
| CN103627652A (en) * | 2013-10-08 | 2014-03-12 | 暨南大学 | Bacillus pumilus for decomposing zearalenone and application thereof |
| CN103981133A (en) * | 2014-05-14 | 2014-08-13 | 中国农业科学院农产品加工研究所 | Bacillus amyloliquefaciens and application thereof in degradation of zearalenone |
| CN105087444B (en) * | 2015-09-02 | 2018-11-23 | 国家粮食局科学研究院 | The bacillus amyloliquefaciens of degrading zearalenone and its application |
| CN105301254A (en) * | 2015-12-22 | 2016-02-03 | 北京勤邦生物技术有限公司 | Immunomagnetic beads for gathering purification of zearalenone and preparing method and application of immunomagnetic beads |
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| CN106721940A (en) * | 2016-12-05 | 2017-05-31 | 江苏大学 | The formulation application of zearalenone saccharomycete in one plant of degraded pig feed |
| CN107873989A (en) * | 2017-11-24 | 2018-04-06 | 黑龙江省农业科学院畜牧研究所 | Remove feed addictive of the dilute ketone toxin of Gibberella zeae and its preparation method and application |
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