CN102550893B - Micro-biological degradation method for zearalenone - Google Patents
Micro-biological degradation method for zearalenone Download PDFInfo
- Publication number
- CN102550893B CN102550893B CN2011104599371A CN201110459937A CN102550893B CN 102550893 B CN102550893 B CN 102550893B CN 2011104599371 A CN2011104599371 A CN 2011104599371A CN 201110459937 A CN201110459937 A CN 201110459937A CN 102550893 B CN102550893 B CN 102550893B
- Authority
- CN
- China
- Prior art keywords
- zen
- powder
- lactobacillus plantarum
- bacillus cereus
- bacterium powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a micro-biological degradation method for zearalenone, and the method can be used for degrading zearalenone by using bacillus cereus and/or Lactobacillus plantarum powder. According to the invention, the zearalenone is degraded by using bacillus cereus and/or Lactobacillus plantarum powder, the degradation effect of the bacillus cereus and/or Lactobacillus plantarum powder to the ZEN is determined under the external simulated gastrointestinal tract environment, the optimum ZEN degrading condition is developed, and powerful guidance can be provided for further developing biological antidote and degrading ZEN in grains and feeds.
Description
Technical field
The invention belongs to the biological detoxification technology field, be specifically related to a kind of microbial degradation method of zearalenone, namely utilize bacillus cereus and Lactobacillus plantarum to come degrading zearalenone.
Background technology
Zearalenone (Zearalenone ZEN), its toxigenic bacterium mainly is the bacterial strain of Fusarium (Fusarium), such as Fusarium graminearum (F.graminearum) and fusarium tricinctum (F.tricinctum); It extensively is present in the cereal crops such as the corn that goes mouldy, Chinese sorghum, wheat, oat, barley and the dairy produce.Used cereals raw material all might be subject to the pollution of ZEN in feed and the food production in field, results process, the rear storage period of results and feed and all too many levels such as food products storage, use.Because zearalenone has estrogen action, can act on reproductive system, makes domestic animal, poultry and experiment mice produce the hyperfunction disease of female hormone; If the edible food that contains zearalenone of gravidic animal (comprising the people) can cause miscarriage, stillborn foetus and monster.Ao Teqi in 2009 shows that to the survey report of the cereal materials of China the recall rate of zearalenone is 88.7%, maximum 7772.3ppb, head and shoulders above the limit standard of China's feed.
Removal method for ZEN on the market mainly includes physical method, chemical method and biological method.In recent years, adopt in the method for microorganism control food chain mycotoxin oneself is paid close attention to by scientific circles, comprise that microbial cells absorption and microorganism are non-toxic products with the toxin metabolism, and the latter's present research emphasis just.
To the common method that adopts in-vitro simulated animal gastrointestinal tract environment of the research of ZEN microbial degradation, namely 37 ℃ is 2.0～2.5 at gastric acid environment pH, cultivates 1～2h; Leading portion enteron aisle acid or alkali environment pH is 6.5～7.0, cultivates 3～4h.Finish the rear ZEN content in the supernatant of measuring by in the microorganism cultivating system, adding a certain amount of ZEN standard items, cultivating, determine whether microorganism can transform or metabolism ZEN.Also have and report the adsorbance that can effectively reduce toxin to the washing times of microbial cells by increasing, and after with an organic solvent processing the microbial cells of cultivating, almost the toxin of being combined with microorganism can both be discharged, these methods all provide certain foundation (Carolyn A h for the effect of scientific verification microbial degradation toxin, HaniS E, Pasi E K, et al.Surface binding of aflatoxin B1 by lactic acid bacteria.Applied and Environmental Microbiology, 2001,67 (7): 3086-3091).But in the research with microbial degradation ZEN, filter out efficient degradation bacteria is one of focus of research always.
Summary of the invention
The microbial degradation method that the purpose of this invention is to provide a kind of zearalenone, namely with bacillus cereus and/or the Lactobacillus plantarum bacterium powder ZEN that degrades, by under in-vitro simulated animal gastrointestinal tract environment, measuring bacillus cereus and/or Lactobacillus plantarum bacterium powder to the degradation effect of ZEN in ZEN standard items and the mouldy corn, determine it to the degradation condition of ZEN, lay a good foundation for further developing ZEN microorganism solution toxic agent.
An object of the present invention is to provide a kind of microbial degradation method of zearalenone, namely come degrading zearalenone with bacillus cereus and/or Lactobacillus plantarum bacterium powder.
Described bacillus cereus is optional, and Classification And Nomenclature is bacillus cereus (Bacillus cereus) available from Chinese common micro-organisms culture presevation administrative center, and preserving number is: CGMCC1.2224.
Described Lactobacillus plantarum is optional, and Classification And Nomenclature is Lactobacillus plantarum (Lactobacillus plantarum) available from Chinese common micro-organisms culture presevation administrative center, and preserving number is: CGMCC1.577.
Another object of the present invention provides a kind of microbial germ powder of degrading zearalenone, and described microbial germ powder is the Mixed Microbes powder of bacillus cereus and Lactobacillus plantarum.
The Mixed Microbes powder of described bacillus cereus and Lactobacillus plantarum, wherein the weight ratio of bacillus cereus and Lactobacillus plantarum is 1: 1-3.
Biodegrading process of the present invention can be degraded to the zearalenone in the musty grain, and concrete is exactly to add bacillus cereus and/or Lactobacillus plantarum bacterium powder comes degrading zearalenone in musty grain.
Biodegrading process of the present invention, the mass percent of the addition of bacillus cereus and/or Lactobacillus plantarum bacterium powder is 0.2%, 1.5h degrades under 37 ℃, pH2.0 condition; Or the mass percent of bacterium powder is 0.2%, and 3.0h degrades under 37 ℃, pH6.5 condition.
The synergy of the Mixed Microbes powder of described bacillus cereus and Lactobacillus plantarum by two bacterial strains can be strengthened the degradation to zearalenone, the degradation effect of Mixed Microbes powder apparently higher than bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder separately to the degradation effect of zearalenone.
The present invention utilizes bacillus cereus and/or Lactobacillus plantarum bacterium powder that zearalenone is degraded, by under in-vitro simulated gastrointestinal tract environment, measuring respectively above-mentioned bacterium powder to the degradation effect of ZEN, find out it to the best degradation condition of ZEN, for further it being developed as the biologic detoxication agent, the degraded that is used for cereal and feed ZEN provides strong guidance.
The specific embodiment
The applied reagent of the present invention can be used arbitrary money commercially available prod, and for example: zearalenone (ZEN) standard items are available from sigma company, and ZEN high-sensitivity detection kit Zearalenone Veratox is available from Britain NEOGEN company; Used buffer solution compound method is as follows:
1, the PBS:KCl:0.2g KH2PO4:0.204g Na2HPO42H2O:1.442gNaCl:8.066g of pH6.5 is dissolved in the 1000ml distilled water, and regulating the pH value is 6.5;
2, the PBS:KCl:0.2g KH2PO4:0.204g Na2HPO42H2O:1.442gNaCl:8.066g of pH2.0 is dissolved in the 1000ml distilled water, and regulating the pH value is 2.0;
3, pepsin solution liquid: accurately take by weighing pepsin (1: 10000) 1.333g, be dissolved in the PBS solution of 10ml pH2.0, fully stirring is dissolved it fully;
4, trypsin solution liquid: accurately take by weighing trypsase (1: 250) 1.0g, be dissolved in the PBS solution of 10ml pH6.5, fully stirring is dissolved it fully.
Below in conjunction with specific embodiment method of the present invention is described in detail.
The preparation of embodiment 1 microbial germ powder
1.1, the preparation of bacillus cereus bacterium powder
1) slant strains
Adopt method of scoring that bacillus cereus CGMCC1.2224 is inoculated into nutrient agar, 37 ℃, cultivated 12 hours;
2) solid seed
From the slant preservation bacterial classification inoculation to the fresh nutrient agar of eggplant bottle inclined-plane, 37 ℃, cultivated 12 hours;
3) preparation of seed liquor
Bacillus cercus is washed from eggplant bottle inclined-plane, be seeded to seeding tank, 37 ℃, 200r/min cultivates 12h;
Culture medium prescription: beancake powder 2.5%, corn steep liquor 2%, sodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;
4) fermented and cultured
The seed liquor of seeding tank is transferred to the 5T fermentation tank culture, and 37 ℃, 150r/min cultivates 20h;
Fermentation tank culture based formulas: beancake powder 2.5%, corn steep liquor 2%, sodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;
5) press filtration: add 3% diatomite in the zymotic fluid, be warmed up to 50 ℃ after, carry out plate compression;
6) oven dry: the bacterium mud after the press filtration is dry in boiling drier, 90 ℃ of intake air temperatures, air outlet temperature 50 C, dry 15s.
7) pulverize: dry thalli granule was pulverized 40 mesh sieves, obtained product.
1.2, the preparation of Lactobacillus plantarum bacterium powder
1) slant strains
Adopt method of scoring that Lactobacillus plantarum CGMCC1.577 is inoculated into nutrient agar, 36 ℃-37 ℃, cultivated 14 hours;
2) solid seed
From the slant preservation bacterial classification inoculation to the fresh nutrient agar of eggplant bottle inclined-plane, 36 ℃-37 ℃, cultivated 14 hours;
3) preparation of seed liquor
Lactobacillus plantarum is washed from eggplant bottle inclined-plane, be seeded to seeding tank, 36 ℃-37 ℃, 200r/min cultivates 14h;
Culture medium prescription: beancake powder 2.5%, corn steep liquor 2%, sodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;
4) fermented and cultured
The seed liquor of seeding tank is transferred to the 5T fermentation tank culture, and 37 ℃, 150r/min cultivates 20h;
Fermentation tank culture based formulas: beancake powder 2.5%, corn steep liquor 2%, sodium hydrogen phosphate 0.1%, ammonium sulfate 0.05%, manganese sulfate 0.05%;
5) press filtration: add 3% diatomite in the zymotic fluid, be warmed up to 50 ℃ after, carry out plate compression;
6) oven dry: the bacterium mud after the press filtration is dry in boiling drier, 90 ℃ of intake air temperatures, air outlet temperature 50 C, dry 15s.
7) pulverize: dry thalli granule was pulverized 40 mesh sieves, obtained product.
Embodiment 2 microbial germ powders are to the degradation of ZEN standard items
2.1 the microbial germ powder degradation time is on the impact of ZEN standard items degradation rate
According to chyme time of staying in animal intestines and stomach, be determined at respectively under simulated animal stomach, the intestinal environment microbial germ powder addition optimum addition of bacterium powder is determined in the impact of ZEN standard items degradation rate.
2.1.1 the microbial germ powder addition is on the impact of ZEN degradation rate in SGF
Add the pH2.0PBS buffer solution 24ml after sterilizing in the triangular flask, add pepsin solution 0.25ml, concentration is the ZEN standard items 0.25ml of 50ppm, by 0.05%, 0.10%, 0.15%, 0.2%, 0.3%, 0.4% (mass ratio) adds respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 1), every group of two repetitions, 37 ℃, 4h is cultivated in concussion, after cultivating end, the centrifugal 10min of 4500r/min, supernatant collect after filtering with funnel, as the liquid to be measured of ZEN in the supernatant; Add 70% methyl alcohol 25ml in the precipitation, concuss 5min filters and collects filtrate, as precipitation desorption ZEN liquid to be measured.Measure front in 4 ℃ of Refrigerator stores.Utilize the ZEN detection kit to measure ZEN content in supernatant and the precipitation, and calculate respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and Mixed Microbes powder to the degradation rate of ZEN.Experimental result sees Table 1
2.1.2 the microbial germ powder addition is on the impact of ZEN degradation rate in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, concentration is the ZEN standard items 0.25ml of 50ppm, by 0.05%, 0.10%, 0.15%, 0.2%, 0.3%, 0.4% (mass ratio) adds respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 1), every group of two repetitions, 37 ℃, 8h is cultivated in concussion, cultivates described in the processing such as step 2.1.1 after finishing.Experimental result sees Table 2
The microbial germ powder addition is on the impact of ZEN standard items degradation rate under the in-vitro simulated gastric environment of table 1
The microbial germ powder addition is on the impact of ZEN standard items degradation rate under the in-vitro simulated intestines environment of table 2
By table 1, table 2 can be found out, Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder all have stronger degradation to the ZEN standard items, and, the Mixed Microbes powder to the degradation effect of ZEN apparently higher than Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder separately separately to the degradation effect of ZEN.Illustrate that Bacillus cercus and Lactobacillus plantarum synergy can be strengthened the degradation to ZEN.
When bacterium powder addition was lower than 0.2%, along with the increase of addition, the ZEN degradation rate improved constantly; When addition was higher than 0.2%, along with the increase of addition, the ZEN degradation rate tended towards stability, so the suitableeest addition of bacterium powder is 0.2%.
2.2 the microbial germ powder degradation time is on the impact of ZEN standard items degradation rate
Under simulated animal stomach, intestinal environment, measure respectively the microbial germ powder degradation time to the impact of ZEN degradation rate, to determine the best degradation time of bacterium powder.
2.2.1 microbial germ powder determining ZEN standard items degradation time in SGF
Add the pH2.0PBS buffer solution 24ml after sterilizing in the triangular flask, add pepsin solution 0.25ml, concentration is the ZEN standard items 0.25ml of 50ppm, the bacillus cereus bacterium powder that adds respectively 0.2% (mass ratio), Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 1), 37 ℃, concussion is cultivated, respectively at 15min, 30min, 45min, 60min, 90min measures the ZEN content in supernatant and the precipitation, every group of two repetitions during 120min, calculate respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and Mixed Microbes powder are to the degradation rate of ZEN, and experimental result sees Table 3.
2.2.2 microbial germ powder determining ZEN standard items degradation time in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, concentration is the ZEN standard items 0.25ml of 50ppm, bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both the Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 1) that add respectively 0.2% (mass ratio), 37 ℃, concussion is cultivated, respectively at 30min, 60min, 90min, 120min, 180min, measure the ZEN content in supernatant and the precipitation during 240min, every group of two repetitions, other steps are together
2.2.1 experimental result sees Table 4.
The microbial germ powder degradation time is on the impact of ZEN standard items degradation rate under the in-vitro simulated gastric environment of table 3
The microbial germ powder degradation time is on the impact of ZEN standard items degradation rate under the in-vitro simulated intestines environment of table 4
By table 3, table 4 can find out that when bacterium powder addition was 0.2%, along with the prolongation of degradation time, the degradation rate of ZEN improved constantly; In in-vitro simulated gastric environment, when degradation time 〉=90min, the degradation rate of ZEN tends towards stability; In in-vitro simulated intestines environment, during degradation time 〉=3h, the degradation rate of ZEN tends towards stability.
In sum, Bacillus cercus bacterium powder, Lactobacillus plantarum bacterium powder and both by the optimum condition of 1: 1 Mixed Microbes powder degraded ZEN standard items are: 0.2%, 37 ℃ of bacterium powder addition, pH2.0, degraded 1.5h; 37 ℃, pH 6.5, degraded 3.0h.The Mixed Microbes powder of bacillus cereus and Lactobacillus plantarum is the highest to the total degradation rate of ZEN standard items, reaches 81.2%, apparently higher than Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder separately separately to the degradation effect of ZEN.
Embodiment 3 microbial germ powders are to the degradation of ZEN in the mouldy corn
3.1 the total quantitative determination of ZEN in the mouldy corn
Cross 20 mesh sieves behind the mouldy crush maize, the assay method mensuration ZEN content that requires according to the Zearalenone Veratox of Britain NEOGEN company (ZEN high-sensitivity detection kit) is: 1536.78ppb
3.2 the microbial germ powder addition is on the impact of ZEN degradation rate in the mouldy corn
According to chyme time of staying in animal intestines and stomach, be determined at respectively under simulated animal stomach, the intestinal environment, the microbial germ powder addition is determined the optimum addition of bacterium powder to the impact of the degradation rate of ZEN in the mouldy corn.
3.2.1 the microbial germ powder addition is on the impact of ZEN degradation rate in the mouldy corn in SGF
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, mouldy corn 10g, by 0.05%, 0.10%, 0.15%, 0.2%, 0.3%, 0.4% (mass ratio) adds respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 3) are set up control group simultaneously, only add mouldy corn, every group of two repetitions, 37 ℃, 4h is cultivated in concussion, after cultivating end, 4500r/min is centrifugal, and supernatant is collected after filtering with funnel, as the liquid to be measured of ZEN in the supernatant; Add 70% methyl alcohol 25ml in the precipitation, concuss 5min filters and collects filtrate, as precipitation desorption ZEN liquid to be measured.Measure front in 4 ℃ of Refrigerator stores.Utilize ZEN content in ZEN high-sensitivity detection kit measurement supernatant and the precipitation, and calculate respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and Mixed Microbes powder to the degradation rate of ZEN, experimental result sees Table 5.
3.2.2 the bacillus cereus addition is on the impact of ZEN degradation rate in the mouldy corn in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, mouldy corn 10g, by 0.05%, 0.10%, 0.15%, 0.2%, 0.3%, 0.4% (mass ratio) adds respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 3), set up simultaneously control group, only add mouldy corn, every group of two repetitions, at 37 ℃, 8h is cultivated in concussion, cultivates described in the processing such as step 3.2.1 after finishing, and experimental result sees Table 6.
The microbial germ powder addition is on the impact of ZEN degradation rate in the mouldy corn under the in-vitro simulated gastric environment of table 5
The microbial germ powder addition is on the impact of ZEN degradation rate in the mouldy corn under the in-vitro simulated intestines environment of table 6
By table 5, table 6 can be found out, Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder all have stronger degradation to ZEN in the mouldy corn, and, the Mixed Microbes powder to the degradation effect of ZEN apparently higher than Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder separately separately to the degradation effect of ZEN.Illustrate that Bacillus cercus and Lactobacillus plantarum synergy can be strengthened the degradation to ZEN in the mouldy corn.
When bacterium powder addition was lower than 0.2%, along with the increase of addition, the ZEN degradation rate improved constantly; When addition was higher than 0.2%, along with the increase of addition, the ZEN degradation rate tended towards stability, so the suitableeest addition of bacterium powder is 0.2%.
3.3 the microbial germ powder degradation time is on the impact of ZEN degradation rate in the mouldy corn
Under simulated animal stomach, intestinal environment, measure respectively microbial germ powder degradation rate to ZEN in the mouldy corn when different degradation time, to determine the best degradation time of microbial germ powder.
3.3.1 microbial germ powder determining ZEN degradation time in the mouldy corn in SGF
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, mouldy corn 10g, the bacillus cereus bacterium powder that adds respectively 0.2% (mass ratio), Lactobacillus plantarum bacterium powder and both Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 3), 37 ℃, concussion is cultivated, respectively at 15min, 30min, 45min, 60min, 90min, measure the ZEN content in supernatant and the precipitation during 120min, set up simultaneously control group, only add mouldy corn, every group of two repetitions, calculate respectively bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and Mixed Microbes powder are to the degradation rate of ZEN, and experimental result sees Table 7.
3.3.2 microbial germ powder determining ZEN degradation time in the mouldy corn in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, mouldy corn 10g, bacillus cereus bacterium powder, Lactobacillus plantarum bacterium powder and both the Mixed Microbes powder (weight ratio of bacillus cereus bacterium powder and Lactobacillus plantarum bacterium powder is 1: 3) that add respectively 0.2% (mass ratio), 37 ℃, concussion is cultivated, respectively at 30min, 60min, 90min, 120min, 180min measures the ZEN content in supernatant and the precipitation, every group of two repetitions during 240min, the same 3.3.1 of other steps, experimental result sees Table 8.
The microbial germ powder degradation time is on the impact of ZEN degradation rate in the mouldy corn under the in-vitro simulated gastric environment of table 7
The microbial germ powder degradation time is on the impact of ZEN degradation rate in the mouldy corn under the in-vitro simulated intestines environment of table 8
By table 7, table 8 can find out that when bacterium powder addition was 0.2%, along with the prolongation of degradation time, the degradation rate of ZEN improved constantly in the mouldy corn; In in-vitro simulated gastric environment, when degradation time 〉=90min, the degradation rate of ZEN tends towards stability; In in-vitro simulated intestines environment, during degradation time 〉=3h, the degradation rate of ZEN tends towards stability.
In sum, Bacillus cercus bacterium powder, Lactobacillus plantarum bacterium powder and both degrade by 1: 3 Mixed Microbes powder, and the optimum condition of ZEN is in the mouldy corn: 0.2%, 37 ℃ of bacterium powder addition, pH2.0, degraded 1.5h; 37 ℃, pH 6.5, degraded 3.0h.The Mixed Microbes powder of bacillus cereus and Lactobacillus plantarum is the highest to the total degradation rate of ZEN in the mouldy corn, reaches 78.4%, apparently higher than Bacillus cercus bacterium powder and Lactobacillus plantarum bacterium powder separately separately to the degradation effect of ZEN.
Claims (6)
1. the microbial degradation method of a zearalenone is characterized in that, is that the bacterium powder with bacillus cereus and Lactobacillus plantarum comes degrading zearalenone, and the weight ratio of bacillus cereus and Lactobacillus plantarum is 1:1-3 in the described bacterium powder.
2. biodegrading process as claimed in claim 1 is to add bacillus cereus and Lactobacillus plantarum bacterium powder comes degrading zearalenone in musty grain.
3. biodegrading process as claimed in claim 2 is characterized in that the mass percent of described bacillus cereus and Lactobacillus plantarum bacterium powder addition in musty grain is 0.2%, and 1.5h degrades under 37 ℃, pH2.0 condition.
4. biodegrading process as claimed in claim 2 is characterized in that the mass percent of described bacillus cereus and Lactobacillus plantarum bacterium powder addition in musty grain is 0.2%, and 3.0h degrades under 37 ℃, pH6.5 condition.
5. microbial germ powder that is used for degrading zearalenone, described microbial germ powder is the Mixed Microbes powder of bacillus cereus and Lactobacillus plantarum.
6. microbial germ powder as claimed in claim 5, the weight ratio that it is characterized in that described bacillus cereus and Lactobacillus plantarum is 1:1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104599371A CN102550893B (en) | 2011-12-31 | 2011-12-31 | Micro-biological degradation method for zearalenone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104599371A CN102550893B (en) | 2011-12-31 | 2011-12-31 | Micro-biological degradation method for zearalenone |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102550893A CN102550893A (en) | 2012-07-11 |
| CN102550893B true CN102550893B (en) | 2013-03-20 |
Family
ID=46398541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104599371A Active CN102550893B (en) | 2011-12-31 | 2011-12-31 | Micro-biological degradation method for zearalenone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102550893B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070209B (en) * | 2013-02-17 | 2014-07-16 | 哈尔滨伟平科技开发有限公司 | Method for mixed dough leavening by multiple strains |
| CN103284023A (en) * | 2013-05-20 | 2013-09-11 | 江南大学 | Application of food-grade microorganism in degradation of zearalenone |
| CN106520593B (en) * | 2016-10-09 | 2019-07-26 | 内蒙古和美科盛生物技术有限公司 | A kind of microorganism formulation that can reduce zearalenone and vomitoxin in ensiling |
| CN108251309B (en) * | 2016-12-29 | 2020-09-01 | 中粮营养健康研究院有限公司 | Bacterium agent and application thereof in degradation of zearalenone |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381688A (en) * | 2008-06-18 | 2009-03-11 | 江苏省农业科学院 | Bacterial strain capable of degrading mold toxin and formulation preparation method thereof |
| CN101792726A (en) * | 2010-03-25 | 2010-08-04 | 山东宝来利来生物工程股份有限公司 | Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin |
| CN102010838A (en) * | 2010-08-27 | 2011-04-13 | 江苏省农业科学院 | Bacterial strain for degrading zearalenone toxin and application thereof |
| CN102028129A (en) * | 2010-12-24 | 2011-04-27 | 北京大北农科技集团股份有限公司 | Novel mycotoxin detoxification agent for feed and preparation method and feed additive thereof |
| CN102132801A (en) * | 2011-03-25 | 2011-07-27 | 南昌大学 | Application of bacillus cereus (E33L) for degrading mycotoxin zearalenone |
| CN102181376A (en) * | 2010-12-23 | 2011-09-14 | 中国农业大学 | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof |
-
2011
- 2011-12-31 CN CN2011104599371A patent/CN102550893B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101381688A (en) * | 2008-06-18 | 2009-03-11 | 江苏省农业科学院 | Bacterial strain capable of degrading mold toxin and formulation preparation method thereof |
| CN101792726A (en) * | 2010-03-25 | 2010-08-04 | 山东宝来利来生物工程股份有限公司 | Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin |
| CN102010838A (en) * | 2010-08-27 | 2011-04-13 | 江苏省农业科学院 | Bacterial strain for degrading zearalenone toxin and application thereof |
| CN102181376A (en) * | 2010-12-23 | 2011-09-14 | 中国农业大学 | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof |
| CN102028129A (en) * | 2010-12-24 | 2011-04-27 | 北京大北农科技集团股份有限公司 | Novel mycotoxin detoxification agent for feed and preparation method and feed additive thereof |
| CN102132801A (en) * | 2011-03-25 | 2011-07-27 | 南昌大学 | Application of bacillus cereus (E33L) for degrading mycotoxin zearalenone |
Non-Patent Citations (2)
| Title |
|---|
| 姜淑贞等.玉米赤霉烯酮的污染和残留及其作用机制.《中国饲料》.2011,(第2期),p41-44. |
| 玉米赤霉烯酮的污染和残留及其作用机制;姜淑贞等;《中国饲料》;20110228(第2期);41-44 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102550893A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106011036B (en) | One plant of bacillus coagulans HEW-B379 and its application with prebiotic effect | |
| CN101864375B (en) | Lactobacillu plantarurn with function of reducing cholesterol and application thereof | |
| CN102181376B (en) | Bacillus subtilis for simultaneously degrading zearalenone and cellulose and application thereof | |
| CN103243047A (en) | Bacillus subtilis capable of effectively degrading vomitoxin and application of bacillus subtilis | |
| CN104770575B (en) | A kind of Radix Astragali probiotics and its preparation method and application | |
| CN103798503B (en) | The ruminant Tiny ecosystem functional feed of composite zymocyte liquid and preparation method | |
| CN108013248A (en) | One boar fermentable fiber feed and preparation method thereof | |
| CN110878265B (en) | Bacillus subtilis for degrading aflatoxin and application thereof | |
| CN104757276B (en) | A kind of probiotics of the lactobacillus-fermented Radix Astragali and its preparation method and application | |
| CN106754579A (en) | A kind of bacillus coagulans and application thereof | |
| CN101792726B (en) | Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin | |
| CN102550893B (en) | Micro-biological degradation method for zearalenone | |
| CN107267408A (en) | A kind of Lactobacillus salivarius JM55 and its application | |
| CN105586300A (en) | Enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus | |
| CN110272891A (en) | Odorless type improvement microbial bacterial agent of kitchen garbage processing and its preparation method and application | |
| CN106957810A (en) | A kind of Pediococcus acidilactici and application thereof | |
| CN111808765B (en) | Bacillus subtilis capable of efficiently degrading vomitoxin and application thereof | |
| CN106635929A (en) | Bacillus subtilis strain and application of antifungal metabolite in grain storage | |
| CN111066946A (en) | Preparation method and application of pelletization-free prawn fermented feed | |
| CN111826298A (en) | Bacillus coagulans for efficiently degrading zearalenone and application thereof | |
| CN109321486A (en) | One plant of lactic acid bacteria and its application with degrading nitrite and biogenic amine | |
| CN110200154A (en) | Pig starter feed of the yeast culture containing antibacterial type and its preparation method and application | |
| CN109266587A (en) | One kind having the active lactobacillus plantarum of degradation of pesticide, preparation method and application | |
| CN102406098B (en) | Method for degrading zearalenone | |
| CN102406100B (en) | Method for degradation of deoxynivalenol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120711 Assignee: Qingdao KDN Biotech Co., Ltd. Assignor: Qingdao Weilan Biology Group Co., Ltd. Contract record no.: 2013370000206 Denomination of invention: Micro-biological degradation method for zearalenone Granted publication date: 20130320 License type: Exclusive License Record date: 20130903 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model |