CN101864375B - Lactobacillu plantarurn with function of reducing cholesterol and application thereof - Google Patents
Lactobacillu plantarurn with function of reducing cholesterol and application thereof Download PDFInfo
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Abstract
The invention discloses Lactobacillu plantarurn with function of reducing cholesterol and application thereof. The strain is Lactobacillu plantarurn C88 with the preservation number of CCTCC NO:M 209254. The strain has strong acid resistance, cholate resistance, antibiotic resistance and adhesiveness, is proved to have good probiotic properties, also has strong performance of reducing the cholesterol, can be used for developing probiotic products for reducing the cholesterol, has the characteristics of producing extracellular Polysaccharide, and can achieve the effects of improving the texture and mouthfeel of products employing the strain. Therefore, the strain has wide application prospect in the process of preparing mucopolysaccharide, capsular polysaccharide and the products with the function of reducing the cholesterol.
Description
Technical field
The present invention relates to plant lactobacillus and application thereof that a strain has the decreasing cholesterol function.
Background technology
Food and Argriculture OrganizationFAO (FAO) and The World Health Organization (WHO) are meant by absorbing suitable amount, to eater's the healthy viable bacteria that can bring into play useful effect the definition of probiotic bacterium.Specifically, the so-called probiotic bacterium arrival small intestine that can live exactly, and can suppress the harmful breeding of bacterium in intestines, and promote intestinal movement, thereby strengthen and keep the Balance of nature of enteron aisle, improve the enteron aisle function.Simultaneously, probiotic bacterium also has a promoter action to healthy, has control diarrhoea, alleviates lactose intolerance, enhancing body immunizing power, preventing cancer and suppress tumor growth and reduce functions such as serum cholesterol.At present, be most that the probiotic bacterium that the people knew and was most widely used is Bacterium lacticum (Lactobacillus) and bifidus bacillus (Bifidobacterium).
Serum cholesterol level is higher to be the primary hazard factor that causes cardiovascular disorder.And the important probiotic properties of probiotic bacterium is to reduce serum cholesterol level.The seventies in 20th century, scientist is successively by to the research of drinking the African Masai human serum cholesterol of cultured milk prods such as yogurt in a large number, to the American investigation of normal drink yogurt, and directly the research of yogurt found that all milk-acid bacteria has the effect that reduces the human serum cholesterol.These results have caused domestic and international investigator's common concern, thereby have started the research boom of milk-acid bacteria decreasing cholesterol effect.So far, there is the different types of milk-acid bacteria of a large amount of experiment confirms to have the effect of reducing cholesterol.Nineteen eighty-two, K.K.Grunewald reports among the 47:2078-2079 in J.Food Sci., and when rat is contained breast through Lactobacterium acidophilum (L.acidophilus) fermentation after 4 weeks by feeding, the rat blood serum cholesterol concentration significantly descends.People such as Danieloson are in J.Anim.Sci. (1989), and report shows the serum cholesterol ability that reduces by the isolated Lactobacterium acidophilum of pig (L.acidophilus) LA16 among the 67:966-974.Usman and Hosono report among the 82:243-248 in J.Dairy Sci. (1999), and that a strain Lactobacillus gasseri (L.gasseri) has is acidproof, bile tolerance and decreasing cholesterol ability.D.I.A.Pereira and G.R.Gibson be in Appl.Environm.Microbiol. (2002), and 68,4689-4693 has reported to separate has good external decreasing cholesterol effect from more HE Bacterium lacticum and bifidus bacillus.M.T.Liong and N.P.Shah be in J.Dairy Sci. (2005), and 88:55-66 has reported Lactobacterium acidophilum and the lactobacterium casei with acidproof, bile tolerance and decreasing cholesterol effect.
Per os enters the probiotic bacterium of body, must be able to tolerate upper gastrointestinal strong acid and high cholate environment, and has good adhesion property and field planting resistance competence exertion prebiotic effect.Therefore can tolerate low pH and high cholate and to the sticking of intestinal epithelial cells, field planting whether as the screening of probiotic bacterium bacterial classification and basis and the prerequisite used.
Summary of the invention
An object of the present invention is to provide the bacterial strain that a strain has the reducing cholesterol function.
Bacterial strain provided by the present invention is plant lactobacillus (Lactobacillus plantarum) C88, and its deposit number is CCTCC NO:M 209254.
Plant lactobacillus (Lactobacillus plantarum) C88 is preserved in Chinese typical culture collection center on November 5th, 2009 and (is called for short CCTCC, address: wuchang, wuhan Luo Jiashan, Wuhan University, postcode 430072), preserving number is CCTCC NO:M 209254.
The application of plant lactobacillus (Lactobacillus plantarum) C88CCTCC NO:M 209254 in reducing cholesterol also belongs to protection scope of the present invention, and described application does not comprise methods for the treatment of diseases.
Plant lactobacillus (L.plantarum) C88CCTCC NO:M 209254 also belongs to protection scope of the present invention in the application that preparation has in the product of reducing cholesterol function.
In the above-mentioned application, described product with reducing cholesterol function can be pharmaceutical composition, makeup with reducing cholesterol function with reducing cholesterol function, has the food of reducing cholesterol function, the pet food that has the animal-feed of reducing cholesterol function or have the reducing cholesterol function.
In the above-mentioned application, described product with reducing cholesterol function is the probiotic products with reducing cholesterol function.
Described probiotic products with reducing cholesterol function is pharmaceutical composition, the makeup with reducing cholesterol function, the food with reducing cholesterol function, the healthcare products with reducing cholesterol function, the starter with reducing cholesterol function with reducing cholesterol function, has the fermented-milk of reducing cholesterol function, the pet food that has the animal-feed of reducing cholesterol function or have the reducing cholesterol function.
Described food with reducing cholesterol function is fluid milk, milk powder, cheese or beverage; Described healthcare products with reducing cholesterol function are capsule.Described fluid milk is a sour milk; Described milk powder is milk powder.
So-called starter is meant to making the synthetic specific microorganisms cultures of sour milk.
The definition of fermented-milk: the acid curd shape product that breast or milk-product form at the effect bottom fermentation of feature bacterium. in the quality guaranteed period, the feature bacterium in this series products must exist in a large number, and can continue to survive and have activity.
Plant lactobacillus (L.plantarum) C88CCTCC NO:M 209254 is at preparation mucus polysaccharide (slimepolysaccharides; SPS) and/or capsular polysaccharide (capsular polysaccharides, CPS) application in also belongs to protection scope of the present invention.
Experiment showed, that bacterial strain of the present invention has the characteristic of producing mucus polysaccharide and capsular polysaccharide; Plant lactobacillus C88CCTCC NO:M 209254 is at 37 ℃ of following well-growns, and 16h reaches logarithmic phase latter stage, and the bacterium number reaches 9.79 (logcfu/ml), and medium pH value drops to 4.07, begins to enter into stationary phase behind the 16h, and 32h reaches stationary phase.Fermentation 56h bacterium number still can reach 8.68 (log cfu/ml).It all is to begin quick growth from 8h that the SPS of plant lactobacillus C88CCTCC NO:M 209254 and CPS produce, and reaches the highest at 32h, and output reaches 22.01mg/L (SPS) and 15.86mg/L (CPS) respectively.After 32h, the amount of SPS and CPS reduced when strain growth roughly was in decline phase.Experiment is proof also, the cholesterol reduced rate average out to 70.82% of plant lactobacillus C88CCTCC NO:M 209254, and when cultivating 12h, the bacterial strain reducing cholesterol is very capable, reaches the highest at 21h, and 24h still keeps very high level; Show that bacterial strain of the present invention has the function of very strong and stable reducing cholesterol, also have the function of reducing cholesterol with its milk-product such as sour milk that prepare.Bacterial strain of the present invention also has stronger acid resistance, bile tolerance, antibiotics resistant and adhesion, prove that this bacterial strain has good probiotic properties, can be used to develop the reducing cholesterol probiotic products, and bacterial strain of the present invention has the characteristic that produces exocellular polysaccharide, can reach improvement and use the quality of this bacterial strain product and the effect of mouthfeel.Therefore, bacterial strain of the present invention will have broad application prospects in the preparation process of the preparation of mucus polysaccharide and capsular polysaccharide, the product of function with reducing cholesterol and probiotic products.
Description of drawings
Fig. 1 is the optical microscope photograph that negative staining making plant Bacterium lacticum C88CCTCC NO:M 209254 produces capsular polysaccharide;
Fig. 2 is plant lactobacillus C88CCTCC NO:M 209,254 37 ℃ of strain growth, glue polysaccharide and capsular polysaccharide productions of cultivating 48h in SDM (semi-defined medium) liquid nutrient medium;
Fig. 3 is the cholate tolerance effect of plant lactobacillus C88CCTCC NO:M 209254.
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Separation obtained bacterial strain of the present invention on December 10th, 2006.
The separation of embodiment 1, bacterial strain and evaluation
One, the separation of bacterial strain
The present invention is the separation screening bacterial strain from the microflora of area, inner mongolia traditional zymotic dried milk cake.
Isolation medium (BCP substratum): yeast extract paste 2.5g/L, peptone 5g/L, glucose 5g/L, purpurum bromocresolis 0.04g/L, 7.0,121 ℃ of sterilizations of agar 15g/L, pH 15min.
Purifying substratum (MRS substratum): peptone 10g/L, extractum carnis 10g/L, yeast extract paste 5g/L, KH
2PO
42g/L, sodium acetate 5g/L, Trisodium Citrate 5g/L, MgSO
47H
2O 0.2g/L, MnSO
44H
2O0.05g/L, tween 80 1mL, agar 15g/L, glucose 20g/L, 6.6,121 ℃ of sterilizations of pH 15min.
Streak culture repeatedly through the BCP separating plate, single colony inoculation that picking produces yellow circle continues to cultivate on the MRS solid medium, through the streak culture repeatedly purifying of MRS flat board, obtains many strains pure culture bacterial classification.
The pure culture bacterial classification filters out many strains lactobacillus through Gram-positive, catalase test feminine gender, indole reaction feminine gender, hydrogen sulfide production test feminine gender, gelatin liquification test feminine gender, hydrolyzed starch test feminine gender and nitrate reduction test feminine gender.Inoculation behind the purifying adds 20% glycerine after cultivating to the MRS liquid nutrient medium, and-80 ℃ of refrigerators are preserved standby.
Measure the output of many strains pure culture bacterial classification exocellular polysaccharide with the phenol sulfuric acid process, filter out the higher bacterial strain of a strain exopolysaccharides, its called after C88.
Two, the evaluation of bacterial strain
(1) physiological and biochemical test
The bacillus that bacterial strain C88 is Gram-positive, catalase feminine gender, do not move, can grow at 15 ℃ and 45 ℃, tolerance 6.5%NaCl, hydrolyzed starch not, liquefy gelatin not, do not produce hydrogen sulfide, glucose fermentation produces not aerogenesis of acid, benzidine test feminine gender, indole test feminine gender, the voges-Proskauer test positive.
(2) API 50CH (French Mei Liai company)
API 50CH qualification result shows that this bacterial strain is plant lactobacillus (L.plantarum), is fabulous evaluation (table 1).
The Physiology and biochemistry of table 1, plant lactobacillus C88 and Microbiological Characteristics
(3) 16S rDNA identifies
16S rDNA sequence is shown in sequence in the sequence table 1, and it reaches 100% with plant lactobacillus JDM1 (GenBankacc.No.CP001617) and EW-p (GenBank acc.No.EU096230) sequence homology reported.
Comprehensive The above results, Bacterium lacticum C88 is accredited as plant lactobacillus (L.plan tarum).
Plant lactobacillus (L.plantarum) C88 is preserved in Chinese typical culture collection center (being called for short CCTCC, address: wuchang, wuhan Luo Jiashan, Wuhan University, postcode 430072) on November 5th, 2009, and preserving number is CCTCC NO:M 209254.
Performance and the function of embodiment 2, plant lactobacillus CCTCC NO.M 209254
One, the growth characteristics of plant lactobacillus CCTCC NO.M 209254 and exocellular polysaccharide produce
Plant lactobacillus C88CCTCC NO:M 209254 is for producing the lactobacillus strain of mucus polysaccharide (SPS) and capsular polysaccharide (CPS) simultaneously.Adopt negative staining microscopic examination plant lactobacillus pod membrane that C88 produces as shown in Figure 1.Usually the polysaccharide that is secreted in the strain growth metabolic process in the substratum is called the mucus polysaccharide, and the polysaccharide that will be attached to the somatic cells wall surface is called capsular polysaccharide, 2 kinds of polysaccharide are distinguished according to existing way is different.
Plant lactobacillus C88CCTCC NO:M 209254 is 37 ℃ of cultivation 48h in SDM (semi-defined medium) liquid nutrient medium, measure strain growth and exopolysaccharides variation in the culturing process, and the result as shown in Figure 2.The result shows, plant lactobacillus C88CCTCC NO:M 209254 is at 37 ℃ of following well-growns, and 16h reaches logarithmic phase latter stage, and the bacterium number reaches 9.79 (log cfu/ml), and medium pH value drops to 4.07, begins to enter into stationary phase behind the 16h, and 32h reaches stationary phase.Fermentation 56h bacterium number (log cfu/ml) still can reach 8.68.It all is to begin quick growth from 8h that the SPS of plant lactobacillus C88CCTCC NO:M 209254 and CPS produce, and reaches the highest at 32h, and output reaches 22.01mg/L (EPS) and 15.86mg/L (CPS) respectively.After 32h, the amount of EPS and CPS reduced when strain growth roughly was in decline phase.
The SDM liquid nutrient medium is prepared as follows: Tryptones 10g (imported L P4002, TRYR Tone), anhydrous sodium acetate 5g, Trisodium Citrate 5g, K
2HPO
42g, MgSO
40.2g, MnSO
40.05g adding distil water adds tween 80 1ml to 1000ml, glucose 20g transfers to pH=6.5 with 1mol/L acetic acid.At 115 ℃ of 15min that sterilize down, with no amino acid nitrogenous source (Yeast Nitrogen Base, BD company) 5g and amino acid (L-His 10mg, L-Met 20mg and L-Try 20mg) mixing through 0.22 μ m filtering with microporous membrane degerming.
Two, the tolerance of plant lactobacillus C88CCTCC NO:M 209254 is measured
1, acid resistance test
In order to determine the tolerance of 209254 pairs of low pH values of Bacterium lacticum C88CCTCC NO:M, be contrast with neutral (pH 6.6), detect the survival rate of bacterial strain in different pH (2.0,2.5 and 3.0).Plant lactobacillus C88CCTCCNO:M 209254 bacterial classification incubated overnight are activated, amount by 3% (v/v) is inoculated in the fresh MRS liquid nutrient medium then, is 2.0,2.5,3.0 and 6.6 with hydrochloric acid with the pH regulator of substratum, place 37 ℃ of incubators to leave standstill cultivation, cultivate bacterium liquid 50 μ L respectively at 1h, 2h and the aseptic taking-up of 3h, coating MRS agar plate is cultivated 48h, survival rate is calculated in enumeration.
The result is as shown in table 2, shows that plant lactobacillus C88CCTCC NO:M 209254 does not tolerate pH 2.0, has good tolerance effect to pH 2.5.PH 2.5 handles 1h～3h survival rate and is respectively 68.57%, 26.83% and 23.26%.PH 3.0 is all had stronger tolerance, and the bacterial strain survival rate still surpasses 80% behind the cultivation 3h.
The acid tolerance effect of table 2, plant lactobacillus C88CCTCC M209254
2. bile tolerance test
In order to check plant lactobacillus C88CCTCC NO:M 209254 to have growth existing state under the environment at cholate, bacterial strain is activated twice in the MRS liquid nutrient medium recover to vigor, 5g/L, 10g/L, 20g/L and 4 gallbladder salinities of 30g/L (bilein that promptly adds different mass in substratum is established in test, obtain the substratum of various biliary salt concn), be contrast with the substratum that does not add cholate.Bacterial strain after the activation is in the MRS liquid nutrient medium, and 37 ℃ of anaerobism are cultivated 24h, are inoculated in respectively in the MRS liquid nutrient medium that contains the different concns bilein by 3% inoculum size.37 ℃ of anaerobism are cultivated 4h, get 50 μ l dilution, coating MRS agar plate.Enumeration behind the cultivation 48h.
The result as shown in Figure 3, the survival rate of 209254 couples of 5g/L of plant lactobacillus C88CCTCC NO:M～30g/L concentration cholate tolerance is respectively 87.91%, 66.42%, 55.31% and 37.57%, experimental result shows, along with gallbladder salinity rising survival rate descends, survival rate can also reach 37.57% under gallbladder salinity 30g/L condition, and this shows that this bacterial strain shows stronger tolerance to cholate.
3. antibiotic resistance is measured
The antibiotics sensitivity of probiotic bacterium plant lactobacillus C88 CCTCC NO:M 209254 adopts minimum inhibition concentration value (MIC) to determine.10 kinds of microbiotic are selected in this test for use, adopt the MRS substratum to add the antibiotic method of different concns, observe and cultivate back bacterium number contrast according to upgrowth situation and obtain this bacterial strain to every kind of antibiotic MIC value.According to MIC value of measuring and the statistical-reference value of the critical point value EUC of MIC (EU Commission), determine that bacterial strain is to various antibiotic susceptibility results.The result is as shown in table 3, shows that except to the Rifampin sensitivity, this bacterial strain demonstrates resistance to the microbiotic of other kinds.
Table 3 plant lactobacillus C88 antibiotics resistance MIC value and susceptibility
S represents responsive, and R represents resistance
Three, cell adhesion is measured
(Greiner Bio-One, when growing to monolayer adherence in Cat.-No.662160), through blood counting plate counting, cell number is 3.5 * 10 at 24 porocyte culture plates when the Caco-2 cell
5Individual.It is 3.4 * 10 that every hole adds 0.5ml concentration
9(GIBCO, Cat.-No.12100-046) nutrient solution are put 37 ℃, 5%CO to the DMEM that CFU/ml bacteria suspension and 0.5ml are fresh
2, 90% humidity CO2gas incubator in hatch 2h, with the PBS rinsing cell 5 times of sterilization, to remove not adherent bacterium.After rinsing is intact, with the fixing 30min of 0.4% Paraformaldehyde 96, gramstaining, microscopy.Add 0.2ml pancreatin effect 10min in every afterwards hole, treat that cell comes off fully, add the nutrient solution termination reaction that 0.125ml contains 20% serum.Add 0.175ml 0.1%Triton X-100 afterwards, leave standstill 10min, with lysing cell.Mixed bacteria liquid behind serial dilution, plate count.Not add bacterium liquid cell cultures is contrast.Each processing do 3 parallel, calculate the average bacterial count of each cell adhesion.The result shows that plant lactobacillus C88 has good adhesivity to the Caco-2 cell, and the adherent cell number is (16.1 ± 0.7) CFU/ cell.
Four, the reducing cholesterol ability is measured
Cholesterol is available from BIO BASIC INC., and catalog number is LS0306B069L CAS#57-88-5; Bovine bile is available from SIGMA.ALDRICH.Co., and catalog number is 8008-63-7;
Picking plant lactobacillus C88CCTCC NO:M 209254 bacterium colonies are transferred in the MRS liquid nutrient medium, cultivate 24h for 37 ℃ and carry out bacterial strain activation, repetition of activation 2 times.
The composition of nutrient solution MRSO-CHOL: form by MRS liquid nutrient medium, Thioglycolic acid sodium salt, cholesterol and bovine bile; The concentration of Thioglycolic acid sodium salt in nutrient solution MRSO-CHOL is 2g/L, and the concentration of cholesterol in nutrient solution MRSO-CHOL is 200mg/L, and the concentration of bovine bile in nutrient solution MRSO-CHOL is 0.3% (quality percentage composition).115 ℃ of sterilization 20min.
Plant lactobacillus C88CCTCC NO:M 209254 reducing cholesterol function detecting methods are as described below: add 10mL nutrient solution MRSO-CHOL (cholesterol level 200mg/L) in the aseptic tool plug of the 15mL centrifuge tube, (concentration of bacterium is 3 * 10 in the bacterium liquid according to bacterium liquid after the inoculum size access activation of 3% (v/v)
9Cfu/ml), cultivate 24h for 37 ℃, the centrifugal 10min of nutrient solution 12000 * g measures cultivation sample supernatant liquor content of cholesterol according to o-phthalaldehyde method, make blank with the nutrient solution MRSO-CHOL that does not connect bacterium, calculate the cholesterol reduced rate of plant lactobacillus C88CCTCC NO:M 209254.
O-phthalaldehyde method: after sample is ready to, get 1mL and place clean tube (18cm * 18cm), add the ethanol of 3mL 95% again adds 2mL 50%KOH solution after the vortex concussion evenly again, and concussion evenly is placed in 60 ℃ the water-bath heats 10min.Take out postcooling to room temperature, add 3mL water, concussion evenly back adds the 5ml normal hexane, and vortex concussion 1min extracts, and leaves standstill 10min under the room temperature.Get normal hexane layer 2.5mL (15cm * 15cm), place 60 ℃ of water-bath N in clean tube
2Dry up (perhaps vacuum-drying), dry back adds 4mL o-phthalaldehyde(OPA) developer, after the concussion evenly, adds the 2mL vitriol oil, leaves standstill 10min behind the concussion 1min.Use spectrophotometer to survey its absorbancy at the 550nm place.
Cholesterol reduced rate (D) calculation formula is:
Wherein A is a test strain fermented supernatant fluid OD550nm value
C is blank OD550nm value
D is the cholesterol reduced rate
3 repetitions are established in experiment, and the result takes the mean.The result is as shown in table 5.It is 68.23% that plant lactobacillus C88CCTCC NO:M 209254 cultivates 12h cholesterol reduced rate, reaches at cultivation 21h to be up to 73.11%, cultivates 24h and still keeps very high level.This shows that this bacterial strain has very strong decreasing cholesterol ability.
Table 5 plant lactobacillus C88 cholesterol reduced rate
Sequence table
＜110〉Jilin Academy of Agricultural Science
＜120〉strain has the plant lactobacillus and the application thereof of decreasing cholesterol function
<160>1
<210>1
<211>1474
<212>DNA
＜213〉plant lactobacillus (Lactobacillus plantarum)
<400>1
gcggcgtgcc taatacatgc aagtcgaacg aactctggta ttgattggtg cttgcatcat 60
gatttacatt tgagtgagtg gcgaactggt gagtaacacg tgggaaacct gcccagaagc 120
gggggataac acctggaaac agatgctaat accgcataac aacttggacc gcatggtccg 180
agcttgaaag atggcttcgg ctatcacttt tggatggtcc cgcggcgtat tagctagatg 240
gtggggtaac ggctcaccat ggcaatgata cgtagccgac ctgagagggt aatcggccac 300
attgggactg agacacggcc caaactccta cgggaggcag cagtagggaa tcttccacaa 360
tggacgaaag tctgatggag caacgccgcg tgagtgaaga agggtttcgg ctcgtaaaac 420
tctgttgtta aagaagaaca tatctgagag taactgttca ggtattgacg gtatttaacc 480
agaaagccac ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt 540
ccggatttat tgggcgtaaa gcgagcgcag gcggtttttt aagtctgatg tgaaagcctt 600
cggctcaacc gaagaagtgc atcggaaact ggaaaacttg agtgcagaag aggacagtgg 660
aactccatgt gtagcggtga aatgcgtaga tatatggaag aacaccagtg gcgaaggcgg 720
ctgtctggtc tgtaactgac gctgaggctc gaaagtatgg gtagcaaaca ggattagata 780
ccctggtagt ccataccgta aacgatgaat gctaagtgtt ggagggtttc cgcccttcag 840
tgctgcagct aacgcattaa gcattccgcc tggggagtac ggccgcaagg ctgaaactca 900
aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagctacgcg 960
aagaacctta ccaggtcttg acatactatg caaatctaag agattagacg ttcccttcgg 1020
ggacatggat acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa 1080
gtcccgcaac gagcgcaacc cttattatca gttgccagca ttaagttggg cactctggtg 1140
agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat 1200
gacctgggct acacacgtgc tacaatggat ggtacaacga gttgcgaact cgcgagagta 1260
agctaatctc ttaaagccat tctcagttcg gattgtaggc tgcaactcgc ctacatgaag 1320
tcggaatcgc tagtaatcgc ggatcagcat gccgcggtga atacgttccc gggccttgta 1380
cacaccgccc gtcacaccat gagagtttgt aacacccaaa gtcggtgggg taacctttta 1440
ggaaccagcc gcctaaggtg ggacagatga ttag 1474
Claims (2)
1. plant lactobacillus (Lactobacillusplantarum) C88, its deposit number is CCTCC NO:M209254.
2. the application of plant lactobacillus (Lactobacillusplantarum) C88 CCTCC NO:M 209254 in preparation mucus polysaccharide and/or capsular polysaccharide.
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