CN103627651A - Bacillus thuringiensis for decomposing zearalenone and application thereof - Google Patents
Bacillus thuringiensis for decomposing zearalenone and application thereof Download PDFInfo
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Abstract
The invention relates to a bacillus thuringiensis for decomposing zearalenone and application thereof. The collection number of the strain disclosed by the invention is CCTCC (China Center for Type Culture Collection) No: M2013409, and a Genbank registry number of the 16s rDNA of the strain is KF512665. During application, a liquid or solid microbial agent capable of degrading zearalenone is prepared from the strain and is used for degrading zearalenone in feed products or grains. The bacillus thuringiensis and the application thereof have the advantages that through bio-degrading zearalenone by using the bacillus thuringiensis, the safety is high, and zearalenone which contaminates the feed products and agricultural products can be degraded in a single-minded and efficient manner, so as to achieve the aim of improving the quality of the feed products.
Description
Technical field
The present invention relates to a kind of bacterial classification that decomposes zearalenone toxin, especially a kind of bacillus thuringiensis of decomposing zearalenone, and the application of this bacterium in feeds product.
Background technology
Zearalenone (Zearalenone, ZEN), claims again F-2 toxin, is a kind of lactone structure of resorcylic acid of phenol, has stronger estrogen activity.This toxin is thermally-stabilised, not because of standing storage, baking or propionic acid or be damaged adding of mould-growth inhibitor.
Zearalenone can bring out a series of estrogen effect symptoms at humans and animals cylinder accumulation, comprise affect that female mammal breast development, vulvovaginitis, oestrus cycle are disorderly, false pregnancy is infertile, miscarriage, stillborn foetus monster etc., in addition studies confirm that, zearalenone also has genetoxic, cytotoxicity, immunotoxicity, causes tumour toxicity.
Zearalenone is present in worldwide various types of grain crop, as: corn, barley, oat, wheat, rice and Chinese sorghum.Before results, the generation of zearalenone does not have the generation of significant amount conventionally, but under suitable envrionment conditions, easily on the corn in storage and little grain, produces.
When using cereal-granules and consuming starch in alcohol production, zearalenone concentrates in fermentation by-product (as: distillation distiller's dried grain).Zearalenone content in fermentation by-product may also can increase with respect to cereal-granules.
At CN102010838A(, adopt bacillus cereus E33L), CN102559555A(adopts acinetobacter calcoaceticus Acinetobacter sp.SM04), CN102796694A(imported the engineering bacteria of deoxynivalenol enol and two kinds of degrading enzyme encoding genes of zearalenone) in disclose respectively and adopt different microorganisms to decompose zearalenone, but the microorganism that they use is not to be put at present and can be used for feed and add the safe microorganisms using.
At CN102406098A(, adopt fermentation of Aspergillus niger liquid dry powder), CN102181376A(adopts subtilis Bacillus subtilis ANSBOIG) in also disclose respectively and use microorganism to decompose the application of zearalenone.
Therefore, be still necessary to find the new microorganism can be used for the decomposition mycotoxins zearalenone that feeds product adds, and be applied to be subject to minute toxolysin in the animal food prods of zearalenone endotoxin contamination.
Summary of the invention
First object of the present invention is to provide a kind of bacillus thuringiensis (Bacillus thuringiensis) of decomposing mycotoxins zearalenone, thereby solves the problem of residual zearalenone toxin in feed.
A kind of bacillus thuringiensis (Bacillus thuringiensis) of decomposing zearalenone of the present invention, the preserving number of its bacterial strain is CCTCC NO:M2013409, depositary institution is Chinese Typical Representative culture collection center (CCTCC), and address is Hubei China province Wuhan City Wuhan University; Preservation date is on September 10th, 2013.Described bacterial strain can be grown on nutrition nutrient agar substratum, and bacterium colony is circular, white, and flat, edge is complete, and bacterium colony has screw thread, and thalline is corynebacterium, and size is 1 μ m * 2～3 μ m, thalline is linked to be chain, Gram-positive; Gemma size is 1.0～1.2 μ m * 1.0～2.0 μ m, ellipse, and middle life or inferior end are raw, and sporangiocyst is not obvious to be expanded; The carbon source that described bacterial strain can utilize has: starch, D-Glucose, Semen Maydis powder, D-lactose, fructose, N-Acetyl-D-glucosamine, arbutin, Vitamin C2, saligenin, cellobiose, maltose, trehalose, glycogen, ribose; Unavailable carbon source has: glycerine, L-arabinose, D-R, erythritol, sucrose, seminose, N.F,USP MANNITOL, gluconate.
Another object of the present invention is to provide the purposes of described bacillus thuringiensis, specifically for the preparation of the application of decomposing the microbial inoculum of zearalenone.
According to the further feature of the application of bacillus thuringiensis of the present invention, described microbial inoculum is liquid bacterial agent, by the following method preparation:
A) bacterial strain of described bacillus thuringiensis is cultivated in seed culture medium, obtained bacterial classification;
B) above-mentioned seed liquor is inoculated into the seeding tank that contains seed culture medium, be cultured to logarithmic phase, obtain seed liquor;
C) seed liquor of acquisition is inoculated into the production ferment tank that contains fermention medium and cultivated, go out tank and form the liquid bacterial agent that can decompose zearalenone.
According to the further feature of the application of bacillus thuringiensis of the present invention, described seed culture medium is for to be comprised of following component: peptone 10.0g/L, beef leaching thing 3.0g/L, NaCl5.0g/L, pH7.0～7.2.
According to the further feature of the application of bacillus thuringiensis of the present invention, described fermention medium is composed of the following components: peptone 1.4g/L, soybean cake powder 32.0g/L, W-Gum 20.0g/L, yeast extract powder 3g/L, CaCO
30.3g/L, KH
2pO
41.0g/L, MgSO
41.0g/L, ZnSO
40.2g/L.
According to the further feature of the application of bacillus thuringiensis of the present invention, described seed culture medium and fermention medium need to be cooled to 30～35 ℃ of uses after 121 ℃ of high-temperature heat sterilizations.
According to the further feature of the application of bacillus thuringiensis of the present invention, described bacterial classification enters seeding tank by the 3-10% inoculum size kind of seeding tank kind seed culture medium, preferably 5% inoculum size, more preferably 10% inoculum size.
According to the further feature of the application of bacillus thuringiensis of the present invention, described seed liquor is inoculated into production tank by the 3-10% inoculum size of producing tank kind fermention medium, preferably 5% inoculum size, more preferably 10% inoculum size.
According to the further feature of the application of bacillus thuringiensis of the present invention, the amount same period at the seed culture of seeding tank and the fermentation culture process kind sterile air of production tank is 1:0.5～1.5, stirring velocity is 100～250 revs/min, incubation time is 24～60 hours, culture temperature is 25～35 ℃, is preferably 28～32 ℃.
According to the further feature of the application of bacillus thuringiensis of the present invention, after fermentation ends, obtain in liquid bacterial agent thalline quantity>=10
9individual/ml, preferably>=10
12individual/ml, or more preferably>=10
15individual/ml.
According to the further feature of the application of bacillus thuringiensis of the present invention, will after described bacillus thuringiensis liquid bacterial agent dilution, spray application in feeds product with the zearalenone toxin in degraded feeds product; In liquid bacterial agent after dilution, thalline quantity at least reaches 10
6individual/ml.
According to the further feature of the application of bacillus thuringiensis of the present invention, described bacillus thuringiensis liquid and bran or clay adsorber are in the ratio absorption of 1ml:1-20g, the preferably ratio of 1ml:5-10g absorption, forms solid fungicide, wherein thalline quantity>=10
8individual/g, preferably>=10
10individual/g, or more preferably>=10
15individual/g.
According to the further feature of the application of bacillus thuringiensis of the present invention, the dosage of described bacillus thuringiensis solid fungicide is 0.5g microbial inoculum/kg feed, preferred 1g microbial inoculum/kg feed, or more preferably 2g microbial inoculum/kg feed.
According to the further feature of the application of bacillus thuringiensis of the present invention, described feed is the feeds product that is selected from following cereal: corn, wheat, barley, paddy, Chinese sorghum or distillation distiller's dried grain.
Beneficial effect of the present invention is: bacillus thuringiensis have strong stress resistance, high temperature resistant, simple to nutritional requirement, easily unique characteristic such as store, the present invention, for polluting the degraded of the zearalenone toxin of feeds product, can effectively reduce the zearalenone toxin in feeds product.
Accompanying drawing explanation
Fig. 1 is the liquid bacterial agent of the bacillus thuringiensis of the present invention degradation effect to zearalenone toxin.
Embodiment
Below the related noun of specification sheets of the present invention is explained.
Zearalenone
Term " zearalenone " comprises the mycotoxins zearalenone producing from some fusarium bacterial classification (Fusarium sp.).IUPAC name is called (4S, 12E)-15,17-dihydroxyl-4-methyl-3-oxabicyclo ［ 12.4.0 ］ 18 carbon-12,15,17,19-tetraene-2,8-diketone ((4S, 12E)-15,17-Dihydroxy-4-methyl-3-oxabicyclo[12.4.0] octadeca-12,15,17,19-tetraene-2,8-dione).Term " zearalenone " also comprises the derivative of any zearalenone.
Animal food prods
Term " animal " comprises all animals, comprises people.The example of animal is ox, includes but not limited to cow/cow and calf; Monogastric animal, for example pig, includes but not limited to piggy, raises pig and sow; Poultry, as turkey and chicken, includes but not limited to chick, laying hen; And fish, include but not limited to salmon.
Term " feeds product " can be the product separated with the zearalenone of undesired level, and it is suitable for by animal edible.Described feeds product can be also the product of the doubtful zearalenone that comprises undesired level, and/or the product of zearalenone level the unknown, comprises the product of the zearalenone that does not comprise section's detection level.
The described feeds product preferably product based on grain preferably comprises cereal, for example, and one or more in corn, wheat, barley, rice, Chinese sorghum.In one embodiment, described feed product can for example only be derived from one or more cereal, and part is derived from beans (being for example derived from soybean) and is partly derived from cereal in another embodiment.The described product based on grain can comprise grain complete or that grind, and for example, the grain of wet-milling or dry grinding, comprises the product based on grain of the fraction that comprises wet-milling or dry grinding grain, described fraction for example, gluten, protein, starch and/or oily fraction.For example also preferably comprise, from brewageing and/or the product of the byproduct (, vinasse) of fermenting process.Vinasse are the byproducts from alcoholic beverage and alcohol fuel production.Beer vinasse are resistatess of brewage in distillery, and this distillery is used malted barley as main raw material.Distillation vinasse be from fermentation grain as: corn, wheat, barley, rice and rye by distillation, remove alcohol after stilling chamber remaining product.Distillation vinasse are also referred to as vinasse.The dry vinasse of producing of the vinasse that will wet, it is mainly as animal-feed.
Bacillus thuringiensis (Bacillus thuringiensis)
What in the context of the present invention, term " bacillus thuringiensis " belonged to that bacillus thuringiensis belongs to has the bacterial strain of Decomposition to zearalenone.
Embodiment 1: the acquisition of bacillus thuringiensis (Bacillus thuringiensis) BFB86335
Material: experiment material is: the old face of leavened food material that Guangzhou Guangdong gathers
Take 10g experiment material and make sample suspension through grinding, add water vibration, gradient dilution obtains sample liquid.Get three each 1mL of concentration gradient sample liquid and add respectively (containing pantoyl internal ester 50mg/L) in nutrition nutrient agar substratum, solidify to be placed in 30 ℃ of thermostat containers and be inverted and cultivate 24h.After cultivation finishes for the first time, the single bacterium colony of the well-grown typical case of picking, under the same conditions, the separated acquisition of line pure culture, carries out classifying and numbering, ℃ preservation of conservation-10, test tube slant.In addition, strengthen pantoyl internal ester concentration in substratum and carry out the second liquid culture enrichment of taking turns to 10g/L.Control group: inoculation is not extremely cultivated containing in the substratum of pantoyl internal ester.Culture condition: 30 ℃, 180r/min, 24h; The pressure that the bacterial strain that can grow is proceeded is below cultivated.
By 1:100 inoculum size, to nutrition bouillon media (being 10 μ g/mL containing ZEN concentration) inoculation, 30 ℃, 180r/min, cultivates 24h; After cultivation finishes, get bacteria suspension 1mL and prepare TLC and detect sample, to ZEN in nutrient solution is residual, carry out TLC detection.Select to detect the corresponding bacterial strain of liquid 10 μ L point sample amount phosphor dot completely dissolve, in being the substratum of 20 μ g/mL, ZEN concentration cultivates, get well-grown bacteria suspension 1mL and prepare TLC detection sample, observe the fluorescent brightness of sample and determine the relative size of each bacterial strain to zearalenone capacity of decomposition, confirm to there is the bacterial strain that decomposes ZEN, determine that name is called BFB86335.
TLC detects with reference to AOAC Official Method976.22: bacteria suspension is through chloroform extraction three times, anhydrous Na2SO4 dehydration, and the TLC obtaining containing residual ZEN detects sample, and point sample on activated silica-gel plate, through developping agent (VCHC
l3: VCH
3oH=95:5) launch, dry, under 365nm UV-light, observe yellow-green fluorescence and change.The point sample that detects sample is respectively 10 μ L, 15 μ L, a 20 μ L3 gradient.
The evaluation of bacterial strain
The 16s rDNA sequence of clone bacillus thuringiensis (Bacillus thuringiensis) BFB86335 also checks order to this 16s rDNA sequence, sequencing result is carried out to BLAST compare of analysis sequence homology at Genbank, determined the systematics status of bacillus thuringiensis (Bacillus thuringiensis) BFB86335.By form biological assay result, physiological and biochemical property result and the systematics status qualification result of bacillus thuringiensis (Bacillus thuringiensis) BFB86335, finally bacillus thuringiensis (Bacillus thuringiensis) BFB86335 is accredited as to bacillus thuringiensis and belongs to (Bacillus thuringiensis) bacterium.The 16s rDNA order of bacterial classification BFB86335 has signed in to Genbank, and accession number is KF512665.
The bacterial strain BFB86335 of this bacillus thuringiensis is delivered to Chinese Typical Representative culture collection center and carry out preservation, preserving number is CCTCC NO:M2013409.
For simplified illustration, hereinafter, the bacterial strain of bacillus thuringiensis of the present invention is represented with BFB86335.
Embodiment 2: the preparation of liquid bacterial agent
Seed culture medium: peptone 10.0g/L, beef leaching thing 3.0g/L, NaCl5.0g/L, pH7.0～7.2.
Fermention medium: peptone 1.4g/L, soybean cake powder 32.0g/L, W-Gum 20.0g/L, yeast extract powder 3g/L, CaCO
30.3g/L, KH
2pO
41.0g/L, MgSO
41.0g/L, ZnSO
40.2g/L.
BFB86335 kind is inoculated in the 1000ml shaking flask containing 200ml seed culture medium, and 30 ℃ of shaking culture, to logarithmic phase, obtain bacterial classification.
Above-mentioned cultured bacterial classification is inoculated to (containing seed culture medium 160L, 121 ℃ of high pressure moist heat sterilizations, are cooled to 30 ℃) in 200L seeding tank by 10% inoculum size and cultivate, obtain seed liquor.Culture condition: 180～230 revs/min of stirring velocitys, sterile air intake 1:0.8, cultivates 6～8 hours.
Above-mentioned seed liquor is inoculated into 2000L production tank (containing fermention medium 1600L, 121 ℃ of high pressure moist heat sterilizations, are cooled to 35 ℃ below) and carried out fermentative production by 10% inoculum size, obtain BFB86335 bacterium liquid.Fermentation condition: 150～200 revs/min of stirring velocitys, sterile air intake 1:0.8～1.0, cultivate 16～45 hours.
Embodiment 3: the preparation example one of solid fungicide
The BFB86335 bacterium liquid that embodiment 2 is produced and bran (from commercial channels buy) are according to 1:5(volume: ratio weight) is mixed and made into BFB86335 solid fungicide.
Embodiment 4: the preparation example two of solid fungicide
The BFB86335 bacterium liquid that embodiment 2 is produced and clay (from commercial channels buy) are according to 1:5(volume: ratio weight) is mixed and made into BFB86335 solid fungicide.
Embodiment 5: the degradation experiment of liquid bacterial agent to zearalenone toxin
By 1:100 inoculum size, to nutrition bouillon media (being 20 μ g/mL containing ZEN concentration) inoculation, 30 ℃, 180r/min, cultivates 24h; After cultivation finishes, get bacteria suspension 5mL and carry out chromatographic analysis.Chromatographic analysis: with reference to AOAC Official Method976.22: bacteria suspension is through chloroform extraction three times, anhydrous Na
2sO
4dehydration, obtains the detection sample containing residual ZEN, gets 1mL detection sample and volatilizes, and adds 1mL methyl alcohol (chromatographically pure) vibration, and 0.45 μ m membrane filtration, obtains testing sample, with the residual ZEN of HPLC detection by quantitative.Chromatographic condition: COSMOSIL5C18-MS II type chromatographic column, moving phase: methanol-water=65:35 (v/v), flow velocity: 1mL/min, column temperature: 30 ℃, RF-10AXL fluorimetric detector: λ ex=274nm, λ em=440nm.As shown in Figure 1, the prepared liquid bacterial agent of bacterial strain of the present invention has good degradation effect for zearalenone toxin to result.
Embodiment 6: the degradation experiment of solid fungicide to zearalenone toxin in feed
BFB86335 solid fungicide mixes with wanting the feed having been polluted by zearalenone of processing by the dosage of 1g solid fungicide/kg dry-matter, then is sprayed into water by 1:1 weight ratio, at 30 ℃, processes after 4 hours, takes 5g and carries out chromatographic analysis.Control group substitutes with the microbial inoculum auxiliary material of equivalent.Chromatographic analysis: with reference to AOACOfficial Method976.22: handled thing is through chloroform extraction three times, anhydrous Na2SO4 dehydration, obtain the detection sample containing residual ZEN, getting 1mL detection sample volatilizes, add 1mL methyl alcohol (chromatographically pure) vibration, 0.45 μ m membrane filtration, obtains testing sample, with the residual ZEN of HPLC detection by quantitative.Chromatographic condition: COSMOSIL5C18-MS II type chromatographic column, moving phase: methanol-water=65:35 (v/v), flow velocity: 1mL/min, column temperature: 30 ℃, RF-10AXL fluorimetric detector: λ ex=274nm, λ em=440nm.With respect to contrast, calculate remaining zearalenone.Result table 1, the zearalenone in the fine degraded feed of BFB86335 solid fungicide energy, degradation efficiency can reach 76.4%.
Table 1
| Test group | Zearalenone content (mg/L) | Degradation rate (%) |
| Control group | 18.47 | -- |
| Add solid fungicide group | 14.32 | 76.4 |
Embodiment 7: the degradation experiment of liquid bacterial agent to zearalenone toxin in feed
After 1000 times of the dosage dilute with waters of BFB86335 liquid bacterial agent 1.0ml bacterium liquid/kg dry-matter, be sprayed onto in the feeds product having been polluted by zearalenone of wanting to process, at 30 ℃, process after 4 hours, take 5g and carry out chromatographic analysis.Control group only substitutes microbial inoculum diluent with the water of equivalent.Chromatographic analysis: with reference to AOACOfficial Method976.22: handled thing is through chloroform extraction three times, anhydrous Na2SO4 dehydration, obtain the detection sample containing residual ZEN, getting 1mL detection sample volatilizes, add 1mL methyl alcohol (chromatographically pure) vibration, 0.45 μ m membrane filtration, obtains testing sample, with the residual ZEN of HPLC detection by quantitative.Chromatographic condition: COSMOSIL5C18-MS II type chromatographic column, moving phase: methanol-water=65:35 (v/v), flow velocity: 1mL/min, column temperature: 30 ℃, RF-10AXL fluorimetric detector: λ ex=274nm, λ em=440nm.With respect to contrast, calculate remaining zearalenone.The results are shown in table 2.Zearalenone in the fine degraded feed of BFB86335 liquid bacterial agent energy, degradation efficiency can reach 79%.
Table 2
| Test group | Zearalenone content (mg/L) | Degradation rate (%) |
| Control group | 18.96 | -- |
| Spraying liquid microbial inoculum group | 14.98 | 79.0 |
Claims (10)
1. a bacillus thuringiensis (bacillus thuringiensis) of decomposing zearalenone, is characterized in that: the preserving number of the bacterial strain of described bacillus thuringiensis is CCTCC NO:M2013409;
Described bacterial strain can be grown on nutrition nutrient agar substratum, and bacterium colony is circular, white, and flat, edge is complete, and bacterium colony has screw thread, and thalline is corynebacterium, and size is 1 μ m * 2～3 μ m, thalline is linked to be chain, Gram-positive; Gemma size is 1.0～1.2 μ m * 1.0～2.0 μ m, ellipse, and middle life or inferior end are raw, and sporangiocyst is not obvious to be expanded;
The carbon source that described bacterial strain can utilize has: starch, D-Glucose, Semen Maydis powder, D-lactose, fructose, N-Acetyl-D-glucosamine, arbutin, Vitamin C2, saligenin, cellobiose, maltose, trehalose, glycogen, ribose; Unavailable carbon source has: glycerine, L-arabinose, D-R, erythritol, sucrose, seminose, N.F,USP MANNITOL, gluconate;
The Genbank accession number of the 16S rDNA complete genome sequence of described bacterial strain is KF512665.
2. bacillus thuringiensis according to claim 1 is for the preparation of the application of decomposing the microbial inoculum of zearalenone.
3. the application of bacillus thuringiensis according to claim 2, is characterized in that: described microbial inoculum is liquid bacterial agent, by the following method preparation:
A) bacterial strain of described bacillus thuringiensis is cultivated in seed culture medium, obtained bacterial classification;
B) above-mentioned seed liquor is inoculated into the seeding tank that contains seed culture medium, be cultured to logarithmic phase, obtain seed liquor;
C) seed liquor of acquisition is inoculated into the production ferment tank that contains fermention medium and cultivated, go out tank and form the liquid bacterial agent that can decompose zearalenone.
4. the application of bacillus thuringiensis according to claim 3, is characterized in that:
Described seed culture medium is for to be comprised of following component: peptone 10.0g/L, beef leaching thing 3.0g/L, NaCl5.0g/L, pH7.0～7.2;
Described fermention medium is composed of the following components: peptone 1.4g/L, soybean cake powder 32.0g/L, W-Gum 20.0g/L, yeast extract powder 3g/L, CaCO
30.3g/L, KH
2pO
41.0g/L, MgSO
41.0g/L, ZnSO
40.2g/L.
5. according to the application of the bacillus thuringiensis described in claim 3 or 4, it is characterized in that: after 121 ℃ of high-temperature heat sterilizations of described seed culture medium and fermention medium needs, be cooled to 30～35 ℃ of uses; Described bacterial classification enters seeding tank by the 3-10% inoculum size kind of seeding tank kind seed culture medium, preferably 5% inoculum size, more preferably 10% inoculum size; Described seed liquor is inoculated into production tank by the 3-10% inoculum size of producing tank kind fermention medium, preferably 5% inoculum size, more preferably 10% inoculum size.
6. the application of bacillus thuringiensis according to claim 3, it is characterized in that: the amount same period at the seed culture of seeding tank and the fermentation culture process kind sterile air of production tank is 1:0.5-1.5, stirring velocity is 100～250 revs/min, incubation time is 24～60 hours, culture temperature is 25～35 ℃, be preferably 28～32 ℃, after fermentation ends, obtain liquid bacterial agent, wherein thalline quantity>=10
9individual/ml, preferably>=10
12individual/ml, or more preferably>=10
15individual/ml.
7. the application of bacillus thuringiensis according to claim 3, is characterized in that: will after described bacillus thuringiensis liquid bacterial agent dilution, spray application in feeds product with the zearalenone toxin in degraded feeds product; In liquid bacterial agent after dilution, thalline quantity at least reaches 10
6individual/ml.
8. the application of bacillus thuringiensis according to claim 3, it is characterized in that: described bacillus thuringiensis liquid and bran or clay adsorber are in the ratio absorption of 1ml:1-20g, the preferably ratio of 1ml:5-10g absorption, forms solid fungicide, wherein thalline quantity>=10
8individual/g, preferably>=10
10individual/g, or more preferably>=10
15individual/g.
9. the application of bacillus thuringiensis according to claim 8, is characterized in that: the dosage of described bacillus thuringiensis solid fungicide is 0.5g microbial inoculum/kg feed, preferred 1g microbial inoculum/kg feed, or more preferably 2g microbial inoculum/kg feed.
10. the application of the bacillus thuringiensis described in claim 7 or 9, is characterized in that: described feed is the feeds product that is selected from following cereal: corn, wheat, barley, paddy, Chinese sorghum or distillation distiller's dried grain.
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| CN105794963A (en) * | 2016-03-14 | 2016-07-27 | 江苏大学 | Saccharomyces cerevisiae degrading zearalenone toxins and application thereof |
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| CN107090420B (en) * | 2017-06-07 | 2021-01-26 | 青岛农业大学 | Fermentation culture method of bacillus thuringiensis |
| CN109938157A (en) * | 2017-12-21 | 2019-06-28 | 中粮生物化学（安徽）股份有限公司 | The method of mycotoxin is removed in distiller's dried grain and its soluble matter production process |
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