CN105838635B - Utilize the method for Pseudomonas fluorescens bacterial strain LZ-4 repairing hexavalent chromium and naphthalene combined pollution environment - Google Patents
Utilize the method for Pseudomonas fluorescens bacterial strain LZ-4 repairing hexavalent chromium and naphthalene combined pollution environment Download PDFInfo
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Abstract
The invention discloses a kind of environmental improvement bacterial strains, are pseudomonas LZ-4 (pseudomonas sp.LZ-4), and in China typical culture collection center preservation, deposit number is CCTCC NO:M 2014257.Bacterial strain of the invention and related microbial inoculum can degradation rate to naphthalene 95% or more, soluble Cr VI Cr (VI) is reduced to insoluble trivalent chromium Cr (III) completely at the same time, solve the problems, such as that Cr (VI)-naphthalene combined pollution is administered, preparation method of the present invention is simple, it is environmentally protective, it is at low cost, use easy to spread.
Description
Technical field
The present invention relates to the preparations of a kind of bacterial strain for handling Cr (VI)-naphthalene combined pollution and related microbial inoculum, belong to for controlling
Manage the microorganism field of environmental pollution.
Background technique
Polycyclic aromatic hydrocarbon (Polycyclic Aromatic Hydrocarbons PAHs) is that typical environment difficult to degrade is dirty
Contaminate object.It represents a kind of industrial maximum polycyclic aromatic of dosage that object naphthalene is the mass production from the byproduct coal tar of coking
Hydrocarbon.It has toxicity, a large amount of emission hazard people and other biological health.Some microorganisms can be raw using naphthalene as sole carbon source
It is long, and degraded and generate nontoxic water and carbon dioxide.
Molecular formula is C10H8, it is crystal that is white, volatile and having special odor under room temperature.Structural formula is as follows:
Heavy metal chromium and compound is widely used in all conglomeraties such as intermetallic composite coating, process hides, printing and dyeing, weaving.Its
Middle Cr VI, that is, Cr (VI) for people and it is various biology have very strong toxicity, have carcinogenesis, can also cause it is many other
Health problem has lasting risk to environment.The Cr (VI) of highly-water-soluble can be reduced into low aqueous solubility by some microorganisms
Trivalent chromium Cr (III) reduces the toxicity of crome metal by reducing its migration.
In short, causing due to the movable increase of human industry and exacerbating organic pollution and heavy metal pollution in environment
Degree, or even the organic matter-heavy metal combined pollution for being difficult to repair is formd, improvement also becomes difficult.At present about
The research of phenol-Cr (VI) combined pollution processing is more.Hunan University Pan Cuiyong mixed cell (Cr (VI) reducing bacteria in 2008
Bacillussp. phenol-Cr (VI) combined pollution waste water is handled with Phenol-degrading Bacteria Strains Pseudomonas putida Migula,
It was found that Bacillussp. can be used as electron donor and carbon source to restore Cr (VI), in fact using the intermediate product that phenol degrading generates
The processing of hybrid bacterial strain Pyrogentisinic Acid-Cr (VI) combined pollution is showed.Song in 2009 etc. reports Pseudomonas
Aeruginosa CCTCCAB91095 can remove phenol and Cr VI Cr (VI) pollution simultaneously.HeFei University of Technology in 2013
Zhou Benjun et al. discovery near certain steel plant pollute riverside sludge in separation screening to pseudomonad pseudomonas
Sp.JF122 can degradation of phenol simultaneously restore Cr (VI), it is believed that the reduction of Cr (VI) be rely on the certain centres of phenol degrading process
Metabolite provides electron donor for it.External someone in 1994 reports catechol can react with Cr (VI), and to it
Mechanism is studied.Heavy metal Cr (VI) and organic matter naphthalene are also the two kinds of pollutants often coexisted in environment.It is domestic at present
Outside there has been no the research for restoring Cr (VI) while single bacterial strain degradation naphthalene, do not administered with single bacterial strain naphthalene-Cr (VI) yet
The report of combined pollution.
Summary of the invention
One of the object of the invention be to provide one plant can while efficient degradation naphthalene reduction of hexavalent chromium Cr (VI) environment
Administer bacterial strain --- pseudomonas (pseudomonassp.) LZ-4.
Second purpose of the invention be to provide related microbial inoculum including pseudomonas (pseudomonassp.) LZ-4 and
Preparation method.
It is multiple in improvement Cr (VI)-naphthalene that third purpose of the present invention is to provide pseudomonas (pseudomonassp.) LZ-4
Close the application of pollution
The invention is realized by the following technical scheme:
A kind of environmental improvement bacterial strain is pseudomonas (pseudomonassp.) LZ-4, in Chinese Typical Representative culture
Collection preservation, deposit number CCTCCNO:M2014257.Depositary institution address are as follows: the Chinese Wuhan Wuhan University.It protects
The hiding date is on June 15th, 2014.
The microbial bacterial agent produced using above-mentioned pseudomonas (pseudomonassp.) LZ-4, active constituent are false
Zygosaccharomyces (pseudomonassp.) LZ-4.
Mentioned microorganism microbial inoculum is solid pharmaceutical preparation or liquid preparation.
The preparation process of mentioned microorganism microbial inoculum includes bacterial strain LZ-4 activation, the preparation of strain, solid or liquid bacterial agent
Preparation.
The preparation process of mentioned microorganism microbial inoculum, specifically comprises the following steps:
(1) activation of bacterial strain: preparation LB liquid medium after high-temperature sterilization, accesses the LZ-4 bacterial strain of cryo-conservation, cultivates
Activation；
(2) preparation of strain: the LZ-4 bacterium solution of aforesaid liquid culture medium culture acquisition is collected by centrifugation, then prepares LB solid
Culture medium accesses the bacterium solution being collected into, is cut into small pieces after cooled and solidified as strain when being cooled to 39-41 DEG C after high-temperature sterilization
- 20 DEG C of refrigerators are placed in save；
(3) preparation of microbial inoculum: the LZ-4 for taking above-mentioned manufactured fritter to save is put into fermentation triangular flask, low whipping speed
32-40h is cultivated at 170-190 revs/min, 27-29 DEG C of cultivation temperature.Ferment tank is turned next to 32-40 hours, in fermentation
After only, by fermentation liquid package encapsulation, liquid bacterial agent is made；
The LZ-4 for taking above-mentioned manufactured fritter to save is put into fermentation triangular flask, and 170-190 revs/min of low whipping speed,
It is cultivated 32-40 hours at 27-29 DEG C of cultivation temperature.Turn next to ferment tank 32-40 hours, after fermentation stops, centrifugation hair
Zymotic fluid, obtains thallus, and the PVA mixing of thawing, stripping and slicing after condensation, package encapsulation are added thereto.
The preparation method of mentioned microorganism microbial inoculum, the incubation step described in step (3) in fermentation triangular flask, is stirred
Mixing speed is preferably 180 revs/min, and incubation time is preferably 36 hours.
The preparation method of mentioned microorganism microbial inoculum, fermentation step in the fermenter described in step (3), fermentation time
Preferably 36 hours.
Mentioned microorganism bacterial strain is applied can administer Cr (VI)-naphthalene combined pollution in environmental improvement.
By experiment it can be seen that pseudomonas (pseudomonassp.) LZ-4 to the degradation rate of naphthalene 90% or more,
At the same time insoluble trivalent chromium Cr (III) is reduced to soluble Cr VI Cr (VI) completely, shows higher processing
Activity.
One bag of LZ-4 solid or liquid bacterial agent are put into membrane bioreaction by the application method of microbial liquid microbial inoculum of the present invention
It answers in device, is passed through waste water, contains Cr (VI) and naphthalene in waste water, open aeration, 28 DEG C of reaction temperature, cultivate 120h, measure waste water
The Cr (VI) of the inside is completely reduced into Cr (III), and naphthalene is all degraded by GC detection.
Pseudomonas (pseudomonassp.) LZ-4 repairs the mechanism of naphthalene pollution are as follows: the bacterial strain is dropped with complete naphthalene
Solve gene cluster nah gene cluster.Bacterium is grown using naphthalene as sole carbon source, and the degrading enzyme for generating degradation naphthalene molecule includes 1,2- bis-
Oxygenase 0nahAa, nahAb, nahAc, nahAd), 1,2- dihydroxy -1,2- dialin dehydrogenase (nahB), 1,2- dihydroxy
Base naphthaline dioxygenase (nahC), 2- hydroxychromen -2- carboxylate isomerase (nahD), trans--O- phenol methylene third
Ketone acid hydrase (nahE), bigcatkin willow aldehyde dehydrogenase (nahF), salicylic acid hydrogenase (nahG), catechol invertase (nahH),
2- hydroxyl dehydrogenase (nahI), 2- hydroxyl glucaric acid invertase (nahJ), decarboxylase (nahK), 2- oxo-obtusilic acid methyl esters water
Synthase (nahL), HTH-typ activating transcription factor (nahR), 4- hydroxyl -2- benzylidene aldehyde lyase (nahM), 2- hydroxyl oneself two
Alkene semialdehyde hydrolase (nahN), acetaldehyde dehydrogenase (nahO), finally enters tricarboxylic acid cycle, is degraded to water and carbon dioxide.
Pseudomonas (pseudomonassp.) LZ-4 repairs Cr (VI) pollution mechanism are as follows: the bacterial strain has known one
A little biology Cr resistances and correlation Cr (VI) reductase, such as A of resistance to chrome protein, the B of resistance to chrome protein, superoxide dismutase, resistance to chrome protein
R, some oxidoreducing enzyme etc..The bacterial strain being capable of reduction of hexavalent chromium Cr (VI) under oxygen existence condition.In the work for the R of resistance to chrome protein
Under, Cr (VI) obtains one and electronically forms transient state pentavalent chromium Cr (V), is finally reduced to Cr (III)；Oxidoreducing enzyme can be straight
It connects catalysis Cr (VI) and is reduced into Cr (III).
And the bacterial strain can generate the centre that catechol etc. has extremely strong reproducibility in the salicylate degradation approach of naphthalene
Redox reaction can occur with Cr (VI) for metabolite, catechol etc., and biocatalysis all restores remaining Cr (VI)
For Cr (III), wherein the enzyme HTH-typ activating transcription factor (nahR) and salicylic acid hydrogenase (nahG) of most critical, correlation drop
Solution mechanism is shown in Fig. 2 and Fig. 3.
The screening separation process of pseudomonas (pseudomonassp.) LZ-4
Acclimating culture medium Bushnell-Haas culture medium prescription (1L distilled water): 5g sodium chloride, 1g biphosphate
Potassium, 1g dipotassium hydrogen phosphate, 1g ammonium nitrate, 0.2g magnesium sulfate, 0.05g iron chloride, 0.02g calcium chloride.It will with 0.2g sodium hydroxide
PH is adjusted to 7.121 DEG C of high pressure steam sterilization 20min.
By pedotheque be added addition concentration be every 100ml liquid contain 1mg Cr VI Cr (VI) and 50mg naphthalene (as
Sole carbon source) BH culture medium in, 28 DEG C of dark culture 7d, experimental group Cr VI is completely reduced, and naphthalene is also completely degraded, so
Repeatedly culture domestication 1 month, the good single bacterium of picking growing way to concentration be every 100ml liquid contain 1mg Cr VI Cr (VI) and
It in the BH culture medium of the naphthalene (as sole carbon source) of 50mg, then isolates and purifies 4 times, high-concentration naphthalene and Cr can be resisted by finally obtaining
(VI) bacterial strain LZ-4, analyzing the bacterial strain and Pseudomonas fluorescens by Vitek has 94% similitude (see Fig. 1).The bacterial strain
16S rDNA sequence and Pseudomonas fluorescens (Pseudomonas thivervalensis) similitude 99%.Illustrate the bacterial strain category
In Pseudomonas fluorescens.
Advantages of the present invention and it is possessed the utility model has the advantages that
(1) the present invention provides one kind can while efficient degradation naphthalene reduction of hexavalent chromium Cr (VI) environmental improvement bacterium
Strain --- pseudomonas (pseudomonassp.) LZ-4.A kind of new selection is provided for environmental treatment.
(2) LZ-4 bacterial strain provided by the invention to the degradation rate of naphthalene 90% or more, at the same time by soluble Cr VI
Cr (VI) is reduced to insoluble trivalent chromium Cr (III) completely, shows higher processing activity, greatly solution Cr (VI)-
The problem that naphthalene combined pollution is administered, and it is environmentally protective.
(3) preparation method of the present invention is simple, at low cost, use easy to spread.
Detailed description of the invention
Fig. 1 is phylogenetic tree of pseudomonas (pseudomonassp.) LZ-4 based on 16S rDNA sequence；
Fig. 2 is the control group that bacterium is not added in pseudomonas (pseudomonassp.) LZ-4；
Fig. 3 is LZ-4+BH+6mM glucose (glucose)+200uMCr (VI), and growth curve and Cr (VI) go back virgin curve；
Fig. 4 is LZ-4+BH+6mMnaphthalene+200uMCr (VI), and growth curve and Cr (VI) go back virgin curve；
Fig. 5 is pseudomonas (pseudomonassp.) LZ-4 to naphthalene molecular degradation access figure；
Fig. 6 is that pseudomonas (pseudomonassp.) LZ-4 acts on lower naphthalene intermediate product catechol reduction Cr (VI)
Biological respinse mechanism figure；
Fig. 7 is 0 hour and 5 hours comparison diagram of pseudomonas (pseudomonassp.) LZ-4.
Specific embodiment
The present invention is explained clearly further with specific embodiment below, but is not constituted in any way to the present invention
Limitation.Experimental method in following embodiments is unless otherwise instructed conventional method.
Embodiment 1 pseudomonas (pseudomonassp.) LZ-4's isolates and purifies
Acclimating culture medium Bushnell-Haas culture medium prescription (1L distilled water): 5g sodium chloride, 1g biphosphate
Potassium, 1g dipotassium hydrogen phosphate, 1g ammonium nitrate, 0.2g magnesium sulfate, 0.05g iron chloride, 0.02g calcium chloride.It will with 0.2g sodium hydroxide
PH is adjusted to 7.121 DEG C of high pressure steam sterilization 20min.
By pedotheque be added addition concentration be every 100ml liquid contain 1mg Cr VI Cr (VI) and 50mg naphthalene (as
Sole carbon source) BH culture medium in, 27-29 DEG C dark culture 7 days, experimental group Cr VI is completely reduced, and naphthalene is also completely degraded,
Culture domestication 1 month repeatedly, the good single bacterium of picking growing way to concentration are that every 100ml liquid contains 1mg Cr VI Cr (VI)
It in the BH culture medium of the naphthalene (as sole carbon source) of 50mg, then isolates and purifies 4 times, high-concentration naphthalene and Cr can be resisted by finally obtaining
(VI) bacterial strain LZ-4, analyzing the bacterial strain and Pseudomonas fluorescens by Vitek has 94% similitude (being shown in Table 1).The bacterial strain
16S rDNA sequence and Pseudomonas fluorescens (Pseudomonas thivervalensis) similitude 99%.
Embodiment 2: the experiment of pseudomonas (pseudomonassp.) LZ-4 reparation Cr (VI)-naphthalene surrounding media
By 5g sodium chloride, 1g potassium dihydrogen phosphate, 1g dipotassium hydrogen phosphate, 1g ammonium nitrate, 0.2g magnesium sulfate, 0.05g iron chloride,
0.02g calcium chloride is added in 1L water.7 are adjusted to sodium hydroxide tune PH.121 DEG C of high pressure steam sterilization 20min, are prepared into
Bushnell-Haas culture medium.
Test 1 group are as follows: the LZ-4 bacterial strain+250mlBH culture medium+6mg naphthalene+inorganic compound containing 500ugCr (VI) ion.
Test 2 groups: LZ-4 bacterial strain+250mlBH culture medium+6mg glucose+inorganic compound containing 500ugCr (VI) ion.Control group
Are as follows: bacterium+250mlBH culture medium+6mg naphthalene+inorganic compound containing 500ugCr (VI) ion is not added.
It after 28 DEG C of dark culture culture 120h, is detected with Electronic Speculum, 1 group of obtained electron microscope of experiment can be seen that Cr (III)
Precipitating.The electron microscope that control group obtains does not have Cr (III) precipitating, it can be seen that LZ-4 bacterial strain can efficiently restore Cr (VI)
It is precipitated at Cr (III), the Cr VI chromium of two groups of experimental and control groups is measured with state quality standard GB 7467-87 the method
The content of Cr (VI) ion, 1 group of sample of experiment are not detected chromium Cr (VI) ion, the sample of 2 groups of experiment chromium compared with the control group
The content decline 60% of Cr (VI) ion.By further detecting, the sample of 1 group of experiment is not detected by gas chromatographic detection naphthalene
Out, and control group does not change.
Embodiment 3: including the activation of pseudomonas (pseudomonassp.) LZ-4:
By 5g sodium chloride, 10g tryptone, 5g yeast extract is added in 1L distilled water, PH is adjusted to 7,121 DEG C of height
It is cooling after pressure steam sterilizing 20min, LB liquid medium is made.By the pseudomonas (pseudomonassp.) of low temperature precious deposits
LZ-4 is inoculated in LB liquid medium with 1% ratio, trains in the case where 27-29 DEG C of mixing speed 180r/min of cultivation temperature
It supports one week.
Embodiment 4: including the preparation of pseudomonas (pseudomonassp.) LZ-4 strain
By 5g sodium chloride, 10g tryptone, 5g yeast extract, 60g agar powder is added in 1L distilled water, PH is adjusted to
It is cooled and solidified after 7,121 DEG C of high pressure steam sterilization 20min, LB solid medium is made.
The fluid nutrient medium comprising pseudomonas (pseudomonassp.) LZ-4 after centrifugation activation, collection include
Then the bacterium solution of LZ-4 bacterial strain prepares LB solid medium, the bacterium being collected into is accessed when being cooled to 39-41 DEG C after high-temperature sterilization
Liquid, cooled and solidified are cut into 3mm*3mm fritter as strain later and are placed in -20 DEG C of refrigerators preservations.
Embodiment 5: including the preparation of pseudomonas (pseudomonassp.) LZ-4 liquid bacterial agent
Take it is above-mentioned made of 3mm*3mm fritter be put into containing LB liquid medium fermentation triangular flask in, low whipping speed 170
Rev/min, 40h is cultivated at 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, ferment 40h,
After fermentation stops, by fermentation liquid package encapsulation, liquid bacterial agent is made；
Embodiment 6: including the preparation of pseudomonas (pseudomonassp.) LZ-4 liquid bacterial agent
3mm*3mm fritter made of Example 4 be put into containing LB liquid medium fermentation triangular flask in, low whipping speed
36h is cultivated at 180 revs/min, 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, fermentation
After fermentation stops, by fermentation liquid package encapsulation, liquid bacterial agent is made in 32h；
Embodiment 7: including the preparation of pseudomonas (pseudomonassp.) LZ-4 liquid bacterial agent
3mm*3mm fritter made of Example 4 be put into containing LB liquid medium fermentation triangular flask in, low whipping speed
32h is cultivated at 190 revs/min, 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, fermentation
36h seals fermentation liquid after fermentation stops in polybag, and liquid bacterial agent is made；
Embodiment 8: including the preparation of pseudomonas (pseudomonassp.) LZ-4 solid fungicide
Take it is above-mentioned made of 3mm*3mm fritter be put into containing LB liquid medium fermentation triangular flask in, low whipping speed 170
Rev/min, 40h is cultivated at 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, ferment 40h,
After fermentation stops, it is centrifuged fermentation liquid, thallus is obtained, the PVA of thawing is added thereto, mixed, stripping and slicing after condensation, with preservative film packet
It wraps up in, is distributed into valve bag.
Embodiment 9: including the preparation of pseudomonas (pseudomonassp.) LZ-4 solid fungicide
3mm*3mm fritter made of Example 4 be put into containing LB liquid medium fermentation triangular flask in, low whipping speed
36h is cultivated at 180 revs/min, 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, fermentation
32h after fermentation stops, is centrifuged fermentation liquid, obtains thallus, the PVA of thawing is added thereto, mixes, stripping and slicing after condensation, and use is fresh-keeping
Film package, is distributed into valve bag.
Embodiment 10: including the preparation of pseudomonas (pseudomonassp.) LZ-4 solid fungicide
3mm*3mm fritter made of Example 4 be put into containing LB liquid medium fermentation triangular flask in, low whipping speed
32h is cultivated at 190 revs/min, 27-29 DEG C of cultivation temperature.It turns next in the fermentor that LB liquid medium is added, fermentation
36h after fermentation stops, is centrifuged fermentation liquid, obtains thallus, the PVA of thawing is added thereto, mixes, stripping and slicing after condensation, and use is fresh-keeping
Film package, is distributed into valve bag.
Embodiment 11:
One bag of LZ-4 liquid bacterial agent is put into the membrane bioreactor containing waste water, 500ugCr is contained in waste water
(VI) and 6mg naphthalene, aeration is opened, 28 DEG C of culture 120h measure the Cr (VI) inside waste water and are completely reduced into Cr (III), naphthalene
It is all degraded by GC detection.
Embodiment 12:
One piece of LZ-4 solid fungicide is put into the membrane bioreactor containing waste water, 500ugCr is contained in waste water
(VI) and 6mg naphthalene, aeration is opened, 28 DEG C of culture 120h measure the Cr (VI) inside waste water and are completely reduced into Cr (III), naphthalene
It is all degraded by GC detection.
Application test is carried out with embodiment 11-12 according to microbial inoculum obtained by embodiment 5-10, testing result is summarized as follows:
Claims (8)
1. a kind of environmental improvement bacterial strain is pseudomonas (pseudomonassp.) LZ-4, protected in Chinese Typical Representative culture
The preservation of hiding center, deposit number are CCTCC NO:M 2014257.
2. the hexavalent chrome reduction enzyme and naphthalene molecular degradation enzyme that are generated by environmental improvement bacterial strain described in claim 1.
3. utilizing the microbial bacterial agent of environmental improvement bacterial strain as described in claim 1 production, it is characterised in that including false unit cell
Pseudomonas (pseudomonassp.) LZ-4.
4. microbial bacterial agent as claimed in claim 3, it is characterised in that be prepared as follows: by pseudomonas
(pseudomonassp.) LZ-4 is inoculated in LB culture medium with 1% ratio, at 27-29 DEG C of cultivation temperature, 180 turns of mixing speed/
In the case where minute, cultivates 7 days, be prepared into solid fungicide or liquid bacterial agent.
5. the preparation method of the microbial bacterial agent as described in claim 3 or 4, it is characterised in that the preparation process of microbial inoculum includes
Bacterial strain LZ-4 activation, the preparation of strain, the preparation of solid or liquid bacterial agent.
6. the preparation method of microbial bacterial agent as claimed in claim 5, it is characterised in that the following steps are included:
(1) activation of bacterial strain: preparation LB liquid medium after high-temperature sterilization, accesses the LZ-4 bacterial strain of cryo-conservation, and culture is lived
Change；
(2) preparation of strain: being collected by centrifugation the LZ-4 bacterium solution of aforesaid liquid culture medium culture acquisition, prepares LB solid medium,
The bacterium solution being collected into is accessed when being cooled to 39-41 DEG C after high-temperature sterilization, is cut into small pieces after cooled and solidified and is placed in -20 as strain
DEG C refrigerator saves；
(3) preparation of microbial inoculum: the LZ-4 for taking above-mentioned manufactured fritter to save is put into fermentation triangular flask, low whipping speed 170-
32-40h is cultivated at 190 revs/min, 27-29 DEG C of cultivation temperature；Turn next to ferment tank 32-40 hours, fermentation stops
Afterwards, by fermentation liquid package encapsulation, liquid bacterial agent is made；
The LZ-4 for taking fritter made of step (2) to save is put into fermentation triangular flask, and 170-190 revs/min of low whipping speed, training
It supports and is cultivated 32-40 hours at 27-29 DEG C of temperature；Turn next to ferment tank 32-40 hours, after fermentation stops, centrifugation fermentation
Liquid, obtains thallus, and the PVA mixing of thawing, stripping and slicing after condensation are added thereto, and solid fungicide is made in package encapsulation.
7. the preparation method of microbial bacterial agent as claimed in claim 6, it is characterised in that in fermentation three described in step (3)
Incubation step in the bottle of angle, mixing speed is preferably 180 revs/min, and incubation time is preferably 36 hours.
8. the preparation method of microbial bacterial agent as claimed in claim 6, it is characterised in that in fermentor described in step (3)
Middle fermentation step, fermentation time are preferably 36 hours.
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| CN113773972B (en) * | 2021-10-08 | 2023-08-29 | 天津科技大学 | Hexavalent chromium reduction strain, method and application thereof |
| EP4174184A1 (en) * | 2021-10-27 | 2023-05-03 | Covestro Deutschland AG | Pseudomonas strain for the manufacture of 1,5-dihydroxynaphthalene |
| CN114703222B (en) * | 2022-03-15 | 2024-02-02 | 上海市农业科学院 | Cultivation method of plant capable of completely degrading polycyclic aromatic hydrocarbon |
| CN115287241B (en) * | 2022-09-15 | 2023-06-09 | 南昌航空大学 | Modified hexavalent chromium reducing bacteria and preparation method and application thereof |
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