CN110343636B - Stachys strain and application thereof in zearalenone degradation - Google Patents
Stachys strain and application thereof in zearalenone degradation Download PDFInfo
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Abstract
The present invention provides a new strain of Stapp (Stappia sp.) YM29 CGMCC No.17759 and its application are disclosed. The invention screens out a Steptop genus (a strain of Steptop) by separating the rhizosphere soil of halophyte in Ulapa poetry region in XinjiangStappia sp.) YM29 CGMCC No.17759, the separated strain has the characteristics of salt resistance, wide growth temperature, easy culture and the like, the liquid fermentation broth of the strain can effectively degrade zearalenone in a culture medium, has obvious and stable technical effects in the application of zearalenone biological decomposition, and has wide application value in the technical field of microbial strain application.
Description
Technical Field
The invention relates to the technical field of microbial strains and application thereof, in particular to a staphyloccocus strain and the technical field of application thereof in zearalenone degradation.
Background
Zearalenone(Zealanenone, ZEN), also known as F-2 toxin, molecular formula C18H22O5The mycotoxin is a common mycotoxin in cereal crops, and pollution of the mycotoxin can not only directly influence the growth of the crops, but also bring harm to animals, but also directly or indirectly transmit the mycotoxin to human bodies through food chains, so that the health of the human bodies is seriously harmed. Zearalenone has reproductive toxicity, immunotoxicity, genetic toxicity and carcinogenicity, and has toxic effects on heart, kidney, liver and lung, and also has influence on nervous system and blood system, which can cause excitement of nervous system and organ hemorrhage, and can also cause death of organism in severe cases. Zearalenone causes disturbances in the body's hormonal balance and may also lead to many diseases of the reproductive system, such as prostate, ovarian, cervical or breast cancer.
Contamination of crops with mycotoxins results in reduced crop yields and possibly also livestock yields, and also extends the scope of contamination by trade spread across the world, which leads to significant economic losses. There are many reports showing severe zearalenone contamination of cereal crops and other foods worldwide and in a study in europe, up to 32% of this mycotoxin has been reported in 5010 samples of mixed cereals. A survey of the incidence of zearalenone in the feed showed a contamination rate of 45% in 5402 samples from australia, 40% in 1402 samples from japan and 49% in 1820 samples from china. The pollution of zearalenone toxin is very common, and causes high attention of all countries.
Researchers at home and abroad research various detoxification techniques in order to reduce zearalenone toxin remained in food and feed, and biological detoxification methods are more and more concerned by people because of being cheaper, safer and more environment-friendly. The biological detoxification method comprises two methods, namely microbial thallus adsorption toxin and microbial degradation toxin. The biodegradation method mainly decomposes, destroys or converts toxic groups of toxins into products with lower toxicity or even non-toxic by using secondary metabolites produced by microorganisms or secreted intracellular and extracellular enzymes through microbial transformation, has the advantages of high specificity, only acts on zearalenone without destroying other ingredients in feed and raw materials, does not affect the taste of food, does not reduce the nutritional value, and has become an important research direction.
In recent years, some progress has been made in the research of treating zearalenone toxin by using a biological method at home and abroad, and some strains with the capacity of clearing zearalenone have been separated and screened. Megharaj M et al, 1997, first reported that a mixed culture of bacteria enriched from soil was able to remove the mycotoxin zearalenone from the culture medium; K.J. Cho et al isolated a bacillus subtilis subspecies from the soil, can effectively degrade zearalenone. Although a certain amount of zearalenone toxin degrading bacteria are found at home and abroad, the types of the separated degrading strains are single, the degrading capability of each strain is different, the degrading mechanism is unclear, the types of degrading enzymes secreted by the degrading bacteria are few, and the application of key enzymes to production is limited. With the continuous development of biotechnology, the technology for removing zearalenone by using microorganisms becomes more and more mature, and it is imperative to explore a biological method for effectively removing zearalenone. Therefore, the screening and development of zearalenone degrading bacteria by utilizing the Xinjiang special habitat have important practical value for solving the problem of zearalenone pollution in grains and feeds.
Disclosure of Invention
Aiming at the problem that the stupor genus is obtained by separating and screening the rhizosphere soil of halophyte in Ulapa poetry area of XinjiangStappia sp.) The present invention provides a new strain, Stepper's strainStappia sp.) YM29 CGMCC No.17759 and its application are disclosed. The invention screens out a Steptop genus (a strain of Steptop) by separating the rhizosphere soil of halophyte in Ulapa poetry region in XinjiangStappia sp.) YM29 CGMCC No.17759, the separated strain has the characteristics of salt resistance, wide growth temperature, easy culture and the like, the liquid fermentation broth of the strain can effectively degrade zearalenone in a culture medium, and the strain has obvious and stable effect in the application of the biological decomposition of zearalenoneThe method has the technical effect of having wide application value in the technical field of microbial strain application.
The invention adopts the main technical scheme that:
the invention specifically provides a Stapp (Stappia sp.) YM29 CGMCC No. 17759. Screening and separating a stapp strain (YM 29) from rhizosphere soil of halophyte in Ulapover area of XinjiangStappia sp.) The strain is a strain of the genus Stapp: (Stappia sp.) YM29 is known to be a new strain through the present common strain identification means, the separated strain has the characteristics of salt tolerance, wide growth temperature, easy culture and the like, the liquid fermentation liquid of the strain has the function of degrading zearalenone in a culture medium, has obvious and stable technical effects in the application of zearalenone biological decomposition, and reflects that the screening of the strain has bright application value guide.
Specifically, according to the particularity of the geographical environment of the Ula berth area in Xinjiang, the invention cultures and separates the microbial strains of rhizosphere soil of halophytes in the Ula berth area in Xinjiang, screens a batch of excellent strains, and separates and screens a Stapp (A) (with the number of YM 29) strain from the excellent strainsStappia sp.) The strain belongs to the genus Stapp through microbiological classification and identification, and the optimal growth conditions of the strain are as follows: 10-45 ℃, and the optimal growth temperature is 30 ℃; the growth range of pH is 5.0-10, 0-10% NaCl tolerance can be achieved, and the culture medium is LB culture medium. According to a common strain identification manual such as Bergey's systematic bacteriology identification manual (ninth edition), morphological, physiological and biochemical tests are carried out on the strain with the number of YM29, and the YM29 strain is determined to be a member in the genus Stapp, but has the characteristics different from the common strains of the member in the genus Stapp, and has the characteristics of some new strains.
Further, the invention carries out gene sequencing on the strain with the number of YM29, the sequence is shown in SEQ ID NO 1 provided after the sequence is attached, the obtained sequence is compared and analyzed through a common website, a phylogenetic tree is constructed, and the comparison and analysis show that the 16S rDNA sequence of the strain YM29 and a standard mode are foundStappia indica B106TThe homology is highest, and the sequence similarity is97.47%, has the distinctive technical characteristics of a new strain, strain YM29 andStappia indica B106Tthe genetic relationship of (2) is recent, a phylogenetic tree is established by a Neighbor-Joining method by utilizing MEGA 5.0 software commonly adopted in the field, and the results are compared and analyzed to find that the strains YM29 and the strains YM29Stappia indica B106TThe confidence value of the strain is 99, the strain is marked to have extremely high support rate as a new strain and excellent stability in the evolutionary tree, and the strain YM29 is determined to be the stapf genus (a), (b), (c), (d) and (d) through the molecular level identification of a series of strainsStappia sp.) The new species, which has the characteristic of typicality of the new species, is tentatively named as Stapp from the taxonomic point of view (Stappia sp.）YM29。
Specifically, the strain YM29 has been deposited in the International depositary organization for microorganisms under the Budapest treaty: china general microbiological culture Collection center (CGMCC). Address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101. the preservation date is 2019, 5 months and 13 days, and the preservation number of the strain is CGMCC No. 17759. Temporarily named as Stapp by microbiological identification (Stappia sp.）YM29。
New species Stapp (Stappia sp.) YM29 grows well on LB-containing culture medium, and forms visible colony after culturing at 30 deg.C for 48h, the colony is small and circular, has diameter of about 1mm, is milky white and translucent, and has glossy and moist surface and smooth edge; the growth temperature range of the thalli is 10-45 ℃, the optimal growth temperature is 30 ℃, the pH growth range is 5.0-10.0, and 0-10% of NaCl can be tolerated.
Further, the present invention provides Stapp: (Stappia) YM29 CGMCC No.17759 has the function of removing zearalenone toxin in the solution, has obvious and stable technical effect and has wide application value.
By implementing the specific technical scheme provided by the invention, the following beneficial effects can be achieved by implementing the content of the invention:
(1) the invention provides a Stapp genus (Stappia sp.) YM29 CGMCC No.17759 is a typical new strainThe optimum growth conditions of the plants are as follows: the growth temperature range is 10-45 ℃, and the optimal growth temperature is 30 ℃; the pH growth range is 5.0-10.0, 0-10% NaCl tolerance is realized, the separated strain has the characteristics of salt resistance, wide growth temperature, easiness in culture and the like, the liquid fermentation liquid of the strain has the effect of effectively degrading zearalenone in a culture medium, and the strain is determined to be Stapusilla (Chi) AStappia sp.) The new species, which has the characteristic of typicality of the new species, is tentatively named as Stapp from the taxonomic point of view (Stappia sp.）YM29。
(2) The separated and screened Steptop of the inventionStappia sp.) YM29 CGMCC No.17759, under the conditions of an optimal culture medium LB culture medium, an optimal culture temperature of 37 ℃, an optimal pH value of 8.0, a bacterial liquid inoculation amount of 2 percent and zearalenone 10 mu g/mL, the highest zearalenone removal rate can reach 92.56 percent, and the high-concentration zearalenone is found to have no obvious inhibition on the growth and degradation of the strain, so that the possibility is provided for the industrial application of the strain to the biological treatment of zearalenone.
Drawings
FIG. 1 shows Stapp: (Stappia sp.) YM29 CGMCC No.17759 phylogenetic tree diagram.
FIG. 2 shows different media for Stapp: (Stappia sp.) YM29 CGMCC No.17759 and zearalenone degradation rate.
FIG. 3 shows Stapp: (A) at various culture temperaturesStappia sp.) YM29 CGMCC No.17759 growth and zearalenone degradation rate diagram.
FIG. 4 shows Stapp: (A) at various initial pH values of the mediumStappia sp.) YM29 CGMCC No.17759 growth and zearalenone degradation rate diagram.
FIG. 5 shows Stapp (Zeta) at various Zearalenone (ZEN) concentrationsStappia sp.) YM29 CGMCC No.17759 growth and zearalenone degradation rate diagram.
FIG. 6 shows Stapp: (Stappia sp.) The growth curve of YM29 CGMCC No.17759 and the degradation power curve chart of zearalenone.
Detailed Description
The present invention will be described below with reference to examples, but the present invention is not limited to the following examples. All raw and auxiliary materials selected for use in the present invention, as well as methods for culturing the selected bacterial species, are well known and used in the art, and all percentages referred to herein are by weight unless otherwise indicated.
The following basic culture media are adopted in the embodiment of the invention:
inorganic salt culture medium: KH (Perkin Elmer)2PO4 1.52g/L，Na2HPO4 2.44g/L，(NH4)2SO4 0.5g/L，MgSO4 0.2g/L，CaCl20.05g/L, agar 20.0g/L, pH 7.2.
Luria-Bertani (LB) Medium: tryptone 10.0g/L, NaCl 10.0g/L, yeast extract 5.0g/L, agar 20.0g/L (solid medium), pH 7.2.
All media were autoclaved at 121 ℃ for 20 minutes.
The first embodiment is as follows: stapp (Stappia sp.) Isolation, screening and identification of YM29
(I) separating and screening:
collecting plant rhizosphere soil samples in Ulapover area of Xinjiang, and separating and purifying microorganisms by a gradient dilution method. Weighing 10g of soil sample, putting the soil sample into 100ml of sterile water, adding sterilized glass beads, rotating at 150rpm and 30 ℃, and shaking for 20min to fully disperse the sample. Taking 100 μ l of supernatant, adding into 900 μ l of sterile water, and diluting with sterile water to obtain 10-2-10-5A dilution of concentration. 100 mul of each concentration diluent is taken and placed in an inorganic salt culture medium containing 10 mu g/mL zearalenone by a conventional coating method in a constant temperature incubator at 30 ℃ for culturing for 72 hours, and after bacteria grow on a flat plate, a single bacterial colony on the flat plate is picked up for purification culture until no foreign bacterial colony exists. A strain with the number of YM29 is preferably selected from the strain, and is transferred to an LB culture medium slant containing 10 mug/mL zearalenone for storage and standby.
(II) classification and identification:
1. stapp (Stappia sp.) Sequencing and analysis of YM 2916S rDNA:
(1) extraction of PCR template DNA:
inoculating the purified strain into LB culture medium of 10 microgram/mL zearalenone, carrying out shake culture at 30 ℃ for 2 days, collecting the thallus, and extracting the total DNA of the genome by adopting a DNA extraction kit.
(2) PCR amplification
Specific primers are adopted:
27F:5'-AGAGTTTGATCCTGGCTCAG-3'，
1492R:5'-GGTTACCTTGTTACGACTT-3'；
the total volume of the PCR reaction system is 25 mu L, and the PCR amplification condition is 94 ℃ for 5 min; 94 ℃ for 45s, 56 ℃ for 45s, 72 ℃ for 45s, 30 cycles; 10min at 72 ℃.
(3) Sequence determination
The PCR amplification product is subjected to electrophoresis detection and purification and then sequenced to obtain a 16S rDNA sequence with the length of 1384bp, the sequence is shown as SEQ ID NO.1 provided after the sequence is attached, the obtained sequence is subjected to comparison analysis through a common NCBI website, a phylogenetic tree is constructed, the phylogenetic tree of the strain is shown as an attached figure 1, and the comparison analysis shows that the strain YM29 and the strain belong to a standard modeStappia indica B106TThe homology is highest, the sequence similarity is 97.47%, a phylogenetic tree is established by a Neighbor-Joining method by utilizing MEGA 5.0 software commonly adopted in the field, and the results are compared and analyzed to find that the strains YM29 and YM29 have the same homology with each otherStappia indica B106TClustering on one branch with a confidence value of 99, showing that the strain has high possibility as a new strain and excellent stability in the evolutionary tree, and determining the strain YM29 as Stapp (Stepe) through molecular level identification of series strainsStappia sp.) The new species, which has the characteristic of typicality of the new species, is tentatively named as Stapp from the taxonomic point of view (Stappia sp.）YM29。
2. Physiological and biochemical assays
(1) St.genus (St.p.) is shown by the results of the growth condition studyStappia sp.) YM29 is a gram-negative, globular bacterium. Strain YM29 was inoculated on an LB plate and cultured at 30 ℃ for 48 hours to form a visible strainThe bacterial colony is small and round, the diameter of the bacterial colony is about 1mm, the bacterial colony is milky white and translucent, the surface of the bacterial colony is glossy and moist, and the edge of the bacterial colony is smooth; the growth temperature range of the thalli is 10-45 ℃, the optimum growth temperature is 30 ℃, the pH growth range is 5.0-10.0, and 0-10% of NaCl can be tolerated.
(2) Catalase and oxidase activities were positive.
(3) Identification using Biolog strain identifier GNII test plate to determine genus Stapp: (Stappia sp.) YM29 can utilize a carbon source of L-arabinose, D-arabitol, D-cellobiose, N-acetyl-D-glucosamine, i-erythritol, D-fructose, D-galactose, gentiobiose, α -D-glucose, m-inositol, maltose, D-mannitol, D-mannose, D-melibiose, D-psicose, D-raffinose/raffinose, L-rhamnose, D-sorbitol, sucrose, turanose, methyl pyruvate, monomethyl succinate, cis-aconitic acid, citric acid, formic acid, D-galacturonic acid, gluconic acid, D-glucamine acid, D-glucuronic acid, α -hydroxybutyric acid, β -hydroxybutyric acid, p-hydroxyphenylacetic acid, p-hydroxyaconitic acid, D-arabinosyl-D-glucitol, D-melilotione, D-fructose, D-, Itaconic acid, alpha-butanoic acid, alpha-pentanoic acid, D, L-lactic acid, malonic acid, propionic acid, quinic acid/cinchona acid, succinic acid, bromosuccinic acid, succinamic acid, L-propylamine amide, D-alanine, L-alanylglycine, L-asparagine, L-aspartic acid, l-glutamic acid, glycyl-L-aspartic acid, glycyl-L-glutamic acid, L-histidine, hydroxy-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-proline, L-pyroglutamic acid, L-serine, L-threonine, gamma-aminobutyric acid, urocanic acid, and glycerol/glycerol.
The results of 16SrDNA sequence analysis, phylogenetic analysis and microbiological characteristic analysis show that the staphylos (A) and (B) provided by the inventionStappia sp.) YM29 is a new bacterial strain of Stapp, and is tentatively named as Stapp (Stappia sp.) YM 29. The strain has been preserved in China general microbiological culture Collection center (CGMCC) in 2019, 5 months and 13 days. Address: the preservation number of the microorganism culture is CGMCC No.17759 at the university road of the sunward area in Beijing, China academy of sciences.
Example two: different media for Stapp (Stappia sp.) Effect of YM29 on degradation of zearalenone
Selecting Stepe genus (A)Stappia sp.) Inoculating YM29 fresh thallus Porphyrae into LB liquid culture medium, and culturing at 30 deg.C for 24 hr at 120r/min in shaking table to obtain seed solution; inoculating the seed solution at 2% into different culture media containing 10 μ g/mL zearalenone, culturing at 30 deg.C for 120h at 120r/min, and determining growth and degradation efficiency of YM 29.
As shown in the attached figure 2, the strain YM29 grows best on LB culture medium, and has the highest zearalenone degradation rate, while the strain grows better on Tryptone Soybean Broth (TSB) and Nutrient Broth (NB), but has lower zearalenone degradation rate; the strains have poor abilities to grow and degrade zearalenone in sea salt Broth (BD).
Example III different incubation temperatures for Stapp: (Stappia sp.) Effect of YM29 on degradation of zearalenone
Selecting fresh lawn, inoculating into LB liquid culture medium, and culturing at 30 deg.C for 24 hr at 120r/min in shaking table to obtain seed liquid; inoculating the seed solution at 2% into LB culture medium containing 10. mu.g/mL zearalenone, culturing at 120r/min for 120h at different temperatures, and determining the growth and degradation efficiency of YM 29.
As shown in the attached figure 3, although the strain can grow at all experimental temperatures, the strain grows better within the temperature of 30-37 ℃, and the OD value of the bacterial liquid is higher. The degradation rate is positively correlated with temperature within 20-37 deg.C, and the rate gradually decreases as the temperature increases to 42 deg.C.
Example four different initial pH vs. Stapp: (Stappia sp.) Effect of YM29 on degradation of zearalenone
Selecting fresh lawn, inoculating into LB liquid culture medium, and culturing at 37 deg.C for 24 hr in shaking table at 120r/min to obtain seed liquid; inoculating the seed solution at 2% into LB culture medium containing 10 μ g/mL zearalenone and adjusting pH, culturing at 120r/min for 120h, and determining growth and degradation efficiency of YM 29.
The test result is shown in figure 4, the growth of the strain YM29 is in negative correlation with the pH value of the culture medium, the concentration of the bacterial liquid is gradually reduced along with the increase of the pH value, the degradation rate of the strain YM29 on zearalenone is in positive correlation with the pH value of the culture medium, the degradation rate is gradually increased along with the increase of the pH value, and the degradation rate of the strain on zearalenone is highest when the pH value is increased to 8.0.
Example five different zearalenone concentrations for Stapp: (Stappia sp.) Effect of YM29 degradation Rate
Selecting fresh lawn, inoculating into LB liquid culture medium, and culturing at 37 deg.C for 24 hr in shaking table at 120r/min to obtain seed liquid; inoculating the seed solution at 2% into LB culture medium containing Zearalenone (ZEN) with different concentrations, culturing at 37 deg.C for 120r/min for 120h, and determining growth and degradation efficiency of YM 29.
As shown in FIG. 5, the growth of strain YM29 was hardly affected by the increase of the zearalenone concentration in the medium, and the degradation rate was positively correlated with the zearalenone concentration in the medium, and the degradation rate was not significantly increased when the zearalenone concentration in the medium was increased from 8. mu.g/mL to 10. mu.g/mL.
Example VI Stapp (Stappia sp.) Kinetics of degradation of zearalenone by YM29
Selecting fresh lawn, inoculating into LB liquid culture medium, and culturing at 37 deg.C for 24 hr in shaking table at 120r/min to obtain seed liquid; inoculating the seed solution 2% into LB culture medium of zearalenone 10 μ g/mL, culturing at 37 deg.C for 10d at 120r/min, and measuring bacterial liquid OD600Value and zearalenone degradation efficiency.
The test results are shown in the degradation and growth curves of FIG. 6. The strain is in slow stage within 0-8 h, the growth amount is less, after 8h, the strain enters logarithmic growth stage, the thallus rapidly grows, and the OD600The value is rapidly increased, the strain gradually enters a stabilization period within 48 hours, the degradation of zearalenone by the strain gradually occurs along with the increase of the strains, but the degradation process mainly occurs in the stabilization period and is cultured to 1The degradation rate of the strain to zearalenone in the culture reaches 92.56% at 44 h.
Stapp provided by the above series of examples (Stappia sp.) YM29 CGMCC No.17759 is a typical new strain, and the optimal growth conditions of the strain are as follows: the growth temperature range is 10-45 ℃, and the optimal temperature growth is 30 ℃; the pH growth range is 5.0-10.0, 0-10% NaCl can be tolerated, the separated strain has the characteristics of salt tolerance, wide growth temperature, easy culture and the like, the liquid fermentation broth of the strain has the effect of effectively degrading the zearalenone in the culture medium, has obvious and stable technical effects in the application of the biological decomposition of the zearalenone, and has wide application value in the technical field of microbial strain application.
The above examples are merely illustrative for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made while remaining within the scope of the present invention.
Sequence listing
<110> institute of microorganism application of Sinkiang academy of agricultural sciences (Xinjiang-Yameiya bioengineering research and development center, China)
<120> a Stapf strain and application thereof in zearalenone degradation
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1384
<212> DNA
<213> genus Stappa (Stappia sp.)
<400> 1
cccatgcgca gcttaccatg cagtcgacgg tctcttcgga ggcagtggca gacgggtgag 60
taacgcgtgg gaacatacct ttcggtacgg aacaacagtt ggaaacgact gctaataccg 120
tatacgccct gagggggaaa gatttatcgc cgagagattg gcccgcgttg gattagctag 180
ttggtggggt aatggcctac caaggcgacg atccatagct ggtctgagag gatgatcagc 240
cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtggg gaatattgga 300
caatgggcgc aagcctgatc cagccatgcc gcgtgagtga tgaaggcctt agggttgtaa 360
agctctttcg ccggtgaaga taatgacggt aaccggaaaa gaagccccgg ctaacttcgt 420
gccagcagcc gcggtaatac gaagggggct agcgttgttc ggaattactg ggcgtaaagc 480
gcacgtaggc tgacttttaa gtcaggggtg aaatcccggg gctcaacctc ggaactgcct 540
ttgatactgg aagtcttgag tccgagagag gtgagtggaa ctccgagtgt agaggtgaaa 600
ttcgtagata ttcggaagaa caccagtggc gaaggcggct cactggctct gtactgacgc 660
tgaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 720
cgatggaagc tagctgtcag gtagcatgct atttggtggc gcagctaacg cattaagctt 780
cccgcctggg gagtacggtc gcaagattaa aactcaaagg aattgacggg ggcccgcaca 840
agcggtggag catgtggttt aattagaagc aacgcgcaga accttaccag cccttgacat 900
gtctggcaca ctccagagat ggggttttcc ctttggggac cggagcacag gtgctgcatg 960
gctgtggtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctct 1020
cccttagttg ccagcattca gttgggcact ctagggggac tgccggtgat aagccgagag 1080
gaaggtgggg aggacgtcaa gtcttcatgg cccctacggg gtgggctaca cacgtgttac 1140
aatggcggtg acaatgggca gagaaggggc gacccggaga taatcttgaa aaaccgtctc 1200
agttcggatt gttctctgca actcgagagc acgaagttgg aatcgctagt aatcgcgtaa 1260
cagcacgacg cggtgaatac gttcccgggc cctgtacaca ccgcccgtca caccatggga 1320
gttggcttta cccgaaggtg gtgcgtaacc cgcaagggag gcagccaacc acgtagtaag 1380
gagg 1384
Claims (2)
1. A compound of the genus StappStappia sp.) Strain YM29, characterized in that it is a strain of the genus Stapp: (A)Stappia sp.) The strain preservation number of the strain YM29 is CGMCC No.17759, and the 16S rDNA gene sequence is shown as SEQ ID NO. 1.
2. Stapp (S) as claimed in claim 1Stappia sp.) Application of strain YM29 in degrading zearalenone toxin.
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