CN113564081A - Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof - Google Patents
Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof Download PDFInfo
- Publication number
- CN113564081A CN113564081A CN202110875403.0A CN202110875403A CN113564081A CN 113564081 A CN113564081 A CN 113564081A CN 202110875403 A CN202110875403 A CN 202110875403A CN 113564081 A CN113564081 A CN 113564081A
- Authority
- CN
- China
- Prior art keywords
- scs
- devorax
- halodurans
- dewasseria
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present disclosure relates to a bacteria of Devorax for degrading vomitoxin, which is classified and named as Devorax halodurans SCS-3(Devosia salina SCS-3), wherein the deposit number of the Devorax halodurans SCS-3 is GDMCC NO: 61245. the strain can be directly used as a donor for producing vomitoxin degrading enzyme, can also be used as a new genetic engineering strain, obtains more excellent characters by receiving external excellent genes, and is used for biodegradation of vomitoxin in the fields of feed, food, livestock raising and the like.
Description
Technical Field
The disclosure relates to the technical field of microorganisms, in particular to Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof.
Background
Vomitoxin (vomitoxin), also known as Deoxynivalenol (DON), is a toxic metabolite produced by fusarium, and belongs to trichothecene toxoid. DON pollution generally occurs in field crops, grain feeding and edible agricultural products, seriously harms human and animal health and causes huge economic loss. The biological method for degrading the mycotoxin has the advantages of strong specificity, high conversion efficiency, no influence on the nutritional value of the feed, avoidance of the regeneration of the toxin and the like, and gradually draws wide attention of researchers. The research shows that the toxicity of DON can be effectively reduced by ring-opening oxidation at C12-13 position, acetylation, glycosylation or oxidation modification at C3 position of DON. Although screening and construction of some vomitoxin degrading bacteria are carried out at present, a certain gap exists from practical application due to the reasons of low degrading capability, poor high-efficiency expression production process, incapability of ensuring safety and the like.
The biological detoxification method specifically degrades vomitoxin and derivatives thereof by using enzyme under mild conditions, does not use harmful chemical reagents, does not have loss of nutrient substances, and avoids secondary pollution. Therefore, screening and excavating new strains for rapidly degrading the vomitoxin to obtain degrading enzyme for efficiently degrading the vomitoxin is an important means for solving the pollution problem of the vomitoxin and recovering the huge loss of the feed industry and the animal husbandry.
Disclosure of Invention
The purpose of the present disclosure is to provide a bacterium producing an emetic toxin-degrading enzyme, which can be used as a donor of the emetic toxin-degrading enzyme directly or as a new genetically engineered strain, and can obtain more excellent properties by receiving an external excellent gene, thereby being used for biodegradation of emetic toxin in the fields of feed, food, livestock and the like.
In one aspect, the present disclosure provides a species of Devorax capable of degrading emetic toxin, wherein the Devorax is classified under the designation Devorax halodurans SCS-3 (Devospora Salina SCS-3), wherein the Devorax halodurans SCS-3 has the deposit number GDMCC NO: 61245.
in another aspect, the present disclosure also provides a method for preparing a vomitoxin-degrading enzyme, wherein the preparation method comprises: inoculating the desmostoc halodurans SCS-3 in the first aspect into a culture medium for culturing to obtain a culture.
In another aspect, the present disclosure also provides a vomitoxin degrading enzyme, wherein the vomitoxin degrading enzyme is prepared by the method of the second aspect.
In another aspect, the present disclosure also provides a method for preparing a genetically engineered bacterium, wherein the method comprises: carrying out genetic engineering modification on wild type Devorax, wherein the wild type Devorax is salt-tolerant Devorax SCS-3, and the preservation number of the salt-tolerant Devorax SCS-3 is GDMCC NO: 61245.
through the technical scheme, the Devowsonia producing the vomitoxin degrading enzyme is obtained, is a new species (Devosia salina) in Devowsonia, and can directly or indirectly obtain the vomitoxin degrading enzyme.
Additional features and advantages of the disclosure will be set forth in the detailed description which follows.
Biological material preservation information
The Devorax halodurans SCS-3 is classified and named as Devosia salina SCS-3, is preserved in Guangdong province microorganism culture collection with the preservation addresses as follows: the preservation date of the No. 59 building 5 of the No. 100 college of the Pieli Zhonglu, Guangzhou city is 2020, 9 and 23 days, and the preservation number is GDMCC NO: 61245.
drawings
The accompanying drawings, which are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure without limiting the disclosure. In the drawings:
FIG. 1 is an electron microscope photograph of Derwortella halodurans SCS-3(Devosia salina SCS-3).
FIG. 2 is a negative staining picture of Derwortella halodurans SCS-3(Devosia salina SCS-3).
FIG. 3 shows the colony morphology of Derwortella halodurans SCS-3(Devosia salina SCS-3).
FIG. 4 is an evolutionary analysis (based on 16S rDNA sequence analysis) between Derworts halodurans SCS-3(Devosia salina SCS-3) and other Derworts members.
FIG. 5 is a determination of the emetic toxin-degrading ability of Derworta halodurans SCS-3(Devosia salina SCS-3).
Detailed Description
The following detailed description of specific embodiments of the present disclosure is provided in connection with the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating the present disclosure, are given by way of illustration and explanation only, not limitation.
In one aspect, the present disclosure provides a species of Derworts capable of degrading emetic toxin, wherein the species of Derworts is designated as Derworts halodurans SCS-3 (Devosea salina SCS-3), wherein the Derworts halodurans SCS-3 (Devosea salina SCS-3) has a deposit number of GDMCC NO: 61245.
according to the disclosure, the 16S rDNA sequence of the desmostoc halodurans SCS-3 is shown as SEQ ID No. 1.
According to the present disclosure, wherein the desmaus halodurans SCS-3 is gram-negative, aerobic, unipolar flagellum, white and rod-shaped.
According to the disclosure, wherein the desmostoc halodurans SCS-3 is beta-galactosidase positive, urease positive, beta-glucosidase positive, gelatinase positive.
According to the present disclosure, wherein said deflocculant SCS-3 salt can be derived from glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose and gluconic acid as carbon sources; alpha-mannosidase, beta-fucosidase, chymotrypsin, alpha-galactosidase and beta-glucuronidase cannot be used as carbon sources, and starch, Tween 80 and casein cannot be hydrolyzed.
According to the disclosure, among the cell wall fatty acids of the salt tolerant DeWasseria SCS-3, C18:1Omega 7C and/or C18:1Omega 6C content 19.49%, C18:1The content of omega 7C11-methyl is 31.41 percent, C16:0The content of (B) was 29.60%.
In another aspect, the present disclosure also provides a method for preparing a vomitoxin-degrading enzyme, wherein the preparation method comprises: inoculating the desmostoc halodurans SCS-3 in the first aspect into a culture medium for culturing to obtain a culture.
Preferably, the culture medium is at least one of LB liquid culture medium and MSM culture medium;
preferably, the culture conditions of the culture include: the culture temperature is 25-30 ℃, the culture time is 2-4 days, the shaking speed is 140-160rpm, and the transfer is performed for 1-3 rounds.
According to the present disclosure, the LB liquid medium comprises, among other components: 5g of yeast extract, 10g of tryptone and 10g of NaCl.
According to the present disclosure, the MSM medium comprises, as components: k2HPO4 2.0g，KH2PO4 1g，MgSO4·7H2O 0.5g，NaNO3 0.5g，(NH4)2SO40.5g, 1mL of filter sterilized trace metal solution (FeCl added per liter)2·4H2O 3g，CoCl2·6H2O 0.38g，MnCl2·4H2O 0.2g，ZnCl2 0.14g，H3BO3 0.124g，Na2MoO4·2H2O 0.072g，NiCl2·6H2O0.048 g and CuCl2·2H2O 0.034g)。
In another aspect, the present disclosure also provides a vomitoxin degrading enzyme, wherein the vomitoxin degrading enzyme is prepared by the method of the second aspect.
In another aspect, the present disclosure also provides a method for preparing a genetically engineered bacterium, wherein the method comprises: carrying out genetic engineering modification on wild type Devorax, wherein the wild type Devorax is salt-tolerant Devorax SCS-3, and the preservation number of the salt-tolerant Devorax SCS-3 is GDMCC NO: 61245.
the present disclosure is further illustrated by the following examples, but is not limited thereto.
Example 1
This example illustrates the preparation and identification of desmausella halodurans SCS-3.
1.0g of the sample (marine sediment sample) was weighed, added to 100mL of physiological saline, resuspended and shaken for 1h, and then the suspension of the sample was pipetted into 50mL of basal Salt Medium (MSM) containing 1. mu.g/mL DON toxin at an inoculum size of 5%, and cultured at 30 ℃ and 180rpm for 7 days. The bacterial suspension was then transferred to 50mL MSM medium containing 5. mu.g/mL DON toxin at 5% inoculum size. The transfer was repeated 4 times and the concentration of DON was continuously increased so that the final concentrations of DON in the medium were 1. mu.g/mL, 5. mu.g/mL, 20. mu.g/mL and 40. mu.g/mL, respectively. The strain is placed upside down in an incubator at 28 ℃ and cultured for 3d to obtain an isolated strain.
The MSM culture medium comprises the following components: k2HPO4 2.0g，KH2PO4 1g，MgSO4·7H2O 0.5g，NaNO3 0.5g，(NH4)2SO40.5g, 1mL of filter sterilized trace metal solution (FeCl added per liter)2·4H2O 3g，CoCl2·6H2O 0.38g，MnCl2·4H2O 0.2g，ZnCl2 0.14g，H3BO3 0.124g，Na2MoO4·2H2O 0.072g，NiCl2·6H2O0.048 g and CuCl2·2H2O0.034 g), and autoclaving at 121 ℃ for 20 min.
The isolated strain was observed as a rod-shaped bacterium under an electron microscope, as shown in FIG. 1; after negative staining, flagella were observed under an electron microscope as shown in FIG. 2. Culturing on an LB culture medium, wherein the culture conditions are as follows: at 28 ℃ for 16h, the colonies appeared white, round, and convex (as shown in FIG. 3).
The physiological and biochemical characteristics of the separated strain are as follows: good gram negative, aerobic, flagellum, milky white and rod-like. Beta-galactosidase positive, urease positive, beta-glucosidase positive, gelatin hydrolysis protease positive. Can use glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose and gluconic acid as carbon sources; can not be expressed by alpha-mannosidase and beta-fucosidaseGlycosidase, chymotrypsin, alpha-galactosidase and beta-glucuronidase are used as carbon sources, and starch, Tween 80 and casein cannot be hydrolyzed. Among the cell wall fatty acids, C18:1Omega 7C and/or C18:1Omega 6C content 19.49%, C18:1The content of omega 7C11-methyl is 31.41 percent, C16:0The content of (B) was 29.60%.
The isolated strain was identified, and it was confirmed to belong to the genus Devorax (Devodia) and named as Devosa halodurans SCS-3 (Devodia salina SCS-3) in test example 1, and whole genome sequencing was performed thereon.
Example 2
This example illustrates that Devorax halodurans SCS-3 has emetic toxin-degrading activity.
The desmaus halodurans SCS-3 separated in the example 1 is inoculated in an LB liquid culture medium, shaking culture and activation are carried out for 1-2d at the temperature of 28 ℃ and the oscillation frequency of 220rpm, and the bacterial liquid is inoculated on an inorganic salt culture medium plate taking vomitoxin as a unique carbon source in a streak way. Setting DON concentration gradient to make the DON final concentration of the inorganic salt culture medium respectively 5 mug/mL, 10 mug/mL and 20 mug/mL. After culturing at 28 ℃ for 72-96h, observing the morphology and growth condition of each strain, and recording the DON tolerance of the strain.
The degradation activity of the salt-tolerant Devorax SCS-3 strain on the DON is detected by HPLC, the seed solution of the Devorax SCS-3 is inoculated into MSM culture medium containing 40 mu g/mL DON as a unique carbon source according to the inoculation amount of 5 percent, and the MSM culture medium is cultured in a shaking table at the temperature of 28 ℃ and the rpm of 220. Sampling every 24h, adding chromatographic grade methanol for half-dilution, centrifuging, taking supernatant, performing HPLC chromatography determination, and detecting the residual content of DON at 220 nm.
The results show that the bacterial strain SCS-3 of Dewolss can grow on an inorganic salt solid medium with vomitoxin as a sole carbon source. The separated Delowski SCS-3 strain has the capability of degrading mycotoxin vomitoxin. HPLC detection results show that the DON degradation rate of the Derworts SCS-3 reaches about 85% at 72h, and the DON degradation rate is almost completely degraded at 96h (FIG. 5).
Example 3
This example sequenced 16S rRNA DNA amplified from the SCS-3 strain of Derwortella.
The genome of the desvorax halodurans SCS-3 strain isolated in example 1 was used
The bacterial genomic DNA of the company was extracted using a kit (centrifugal column). Design of a general primer sequence for PCR amplification of DNA of 16S rRNA of DeWasser SCS-3 Strain the primers were synthesized by Heihua Dagenetechnology, Inc. of Beijing and the kit for amplification was purchased from Novozam Biotechnology, Inc. of Nanjing, to reference William G.Weisburg (1991).
Forward primer
27F-(5’-AGAGTTTGATCATGGCTCAG-3’，SEQ ID NO.2)，
Reverse primer
1492R(5’-TACGGTTACCTTGTTACGACTT-3’，SEQ ID NO.3)；
Table 116S rDNA amplification system:
| composition (I) | Volume/. |
| 2×Phanta |
25 |
| |
2 |
| |
2 |
| |
2 |
| Sterilization ddH2O | The volume is fixed to 50 mu L |
TABLE 2 PCR reaction procedure
Connecting the amplified 16S rRNA DNA sequence to
Coli DH 5. alpha. competent cells were introduced into pJET 1.2/blunt vector from Inc., positive seeds were selected on LB solid plate containing ampicillin, the inserted sequence was sequenced, and the nucleotide sequence obtained by the sequencing was shown in SEQ ID NO: 1.
Test example 1
The 16S rDNA of the Delowski SCS-3 separated in the example 1 has a significant difference compared with the 16S rDNA of other species in the same genus:
and Devosia riboflavina IFO13584TThe similarity of (a) is 98.22%;
and Devosia indica IO390501TThe similarity of (a) is 97.72%;
and Devosia elaeis S37TThe similarity of (a) is 97.31%;
with Devosia soli GH2-10TThe similarity of (a) was 97.22%;
with Devosia subaequoris HST3-14TThe similarity of (a) was 97.22%;
and Devosia chinhatensis IPL18TThe similarity of (a) is 97.01%;
and Devosia crocina IPL20TThe degree of similarity of (a) is 96.94%, and so on.
The constructed phylogenetic tree is shown in FIG. 4.
Test example 2
Example 1The separated SCS-3 strain of Devorax and its related strain Devosia riboflavina IFO13584T、Devosia indica IO390501TThe comparison of the microbiological properties (see table 3) shows that the microbiological properties of the strains obtained in this example are significantly different from those of the closely related strains.
TABLE 3 comparison of microbial Properties of the Devorax SCS-3 with different closely related strains of the same genus
Note: "+" indicates positive, "-" indicates negative, and "w" indicates weak positive.
The results of test examples 1 and 2 can demonstrate that the obtained desmausella halodurans SCS-3 of the present disclosure is a novel species (devosis salina) in degausia (devosis) and has a function of degrading vomitoxin.
The preferred embodiments of the present disclosure are described in detail with reference to the accompanying drawings, however, the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present disclosure within the technical idea of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.
It should be noted that, in the foregoing embodiments, various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various combinations that are possible in the present disclosure are not described again.
In addition, any combination of various embodiments of the present disclosure may be made, and the same should be considered as the disclosure of the present disclosure, as long as it does not depart from the spirit of the present disclosure.
Sequence listing
<110> institute of biotechnology of Chinese academy of agricultural sciences
<120> Devorax SCS-3 producing vomitoxin degrading enzyme and application thereof
<140> 20235CAAS-B-ZW
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1441
<212> DNA
<213> Devorax halodurans (Devosia salina SCS-3)
<400> 1
ttaccttgtt tcgacttcac cccagtcgct gaccctaccg tggtcggctg cttccttgcg 60
gttagcgcac cggcttcggg taaaaccaac tcccatggtg tgacgggcgg tgtgtacaag 120
gcccgggaac gtattcaccg cagcatgctg atctgcgatt actagcgatt ccaacttcat 180
gcacccgagt tgcagagtgc aatccgaact gagacggttt ttcgggatta gcatcatgtc 240
gccatgtagc tgccctctgt caccgccatt gtagcacgtg tgtagcccag cccataaggg 300
ccatgatgac ttgacgtcat ccccaccttc ctccggctta tcaccggcag tctccttaga 360
gtgcccaact aaatgatggc aactaaggac gagggttgcg ctcgttgcgg gacttaaccc 420
aacatctcac gacacgagct gacgacagcc atgcagcacc tgtgtgtcag tcaccgaagt 480
gaaggaatcc atctctggaa accgtccgac catgtcaagg gctggtaagg ttcttcgcgt 540
tgcttcgaat taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt 600
tttaatcttg cgaccgtact ccccaggcgg agagcttaat gcgttagctg cgccactgag 660
tggtaaacca cccaacggct agctctcata gtttacggcg tggactacca gggtatctaa 720
tcctgtttgc tccccacgct ttcgcacctc agcgtcagtt ccggaccagt aagccgcctt 780
cgccactggt gttcttccta atatctacga attccacctc tacactagga gttccactta 840
cctcttccgg actctagctt gccagtatca aaggcagttc cggagttgag ctccgggatt 900
tcacctctga cttaacaaac cgcctacgtg cgctttacgc ccagtaaatc cgaacaacgc 960
tagccccctt cgtattaccg cggctgctgg cacgaagtta gccggggctt cttctccgac 1020
taccgtcatt atcttcatcg gtgaaagagc tttacaaccc taaggccttc atcactcacg 1080
cggcatggct ggatcaggct tgcgcccatt gtccaatatt ccccactgct gcctcccgta 1140
ggagtctggg ccgtgtctca gtcccagtgt ggctgatcat cctctcagac cagctaaaga 1200
tcgtcgcctt ggtaggccat taccccacca actagctaat cttacgcggg ctcatccaat 1260
tccgataaat ctttcccccg tagggcgtat acggtattag cagtcgtttc caactgttgt 1320
tccgtagaac tgggtagatt cccacgcgtt actcacccgt ctgccactcc ccttgcgggg 1380
cgttcgactt gcatgtgtta agcctgccgc cagcgttcgt tctgagccag gatcaaactc 1440
t 1441
<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
<210> 3
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
tacggttacc ttgttacgac tt 22
Claims (10)
1. Devorax capable of degrading emetic toxin, wherein the Devorax is classified and named Devorax halodurans SCS-3(Devosia salina SCS-3), wherein the deposit number of the Devorax halodurans SCS-3 is GDMCC NO: 61245.
2. the DeWasseria according to claim 1, wherein the 16S rDNA sequence of the DeWasseria halodurans SCS-3 is shown in SEQ ID No. 1.
3. The DeWasseria sp.as claimed in claim 1, wherein the DeWasseria halodurans SCS-3 is gram negative, aerobic, flagellated, white and rod-shaped.
4. The DeWasseria halodurans SCS-3 of claim 1, wherein the DeWasseria halodurans SCS-3 is β -galactosidase positive, urease positive, β -glucosidase positive, gelatinase positive.
5. The DeWasseria halodurans of claim 1, wherein the DeWasseria halodurans SCS-3 is capable of donating a carbon source selected from the group consisting of glucose, arabinose, mannose, mannitol, N-acetyl-glucosamine, maltose, and gluconic acid; alpha-mannosidase, beta-fucosidase, chymotrypsin, alpha-galactosidase and beta-glucuronidase cannot be used as carbon sources, and starch, Tween 80 and casein cannot be hydrolyzed.
6. The Devorax halodurans according to claim 1, wherein in the cell wall fatty acid of Devorax halodurans SCS-3, C is18:1Omega 7C and/or C18:1Omega 6C content 19.49%, C18:1The content of omega 7C11-methyl is 31.41 percent, C16:0The content of (B) was 29.60%.
7. A method of preparing a vomitoxin-degrading enzyme, wherein the preparation method comprises: the culture medium inoculated with the SCS-3 of the salt-tolerant Devorax facilis of any one of claims 1 to 6 for culture.
8. The method of claim 7, wherein the medium is at least one of LB liquid medium, MSM medium; the culture conditions of the culture include: the culture temperature is 25-30 ℃, the culture time is 2-4 days, the shaking speed is 140-160rpm, and the transfer is performed for 1-3 rounds.
9. An emetic toxin-degrading enzyme prepared by the method of claim 7 or 8.
10. A method for preparing a genetically engineered bacterium, wherein the method comprises: carrying out genetic engineering modification on wild type Devorax, wherein the wild type Devorax is salt-tolerant Devorax SCS-3, and the preservation number of the salt-tolerant Devorax SCS-3 is GDMCC NO: 61245.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110875403.0A CN113564081B (en) | 2021-07-30 | 2021-07-30 | Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110875403.0A CN113564081B (en) | 2021-07-30 | 2021-07-30 | Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113564081A true CN113564081A (en) | 2021-10-29 |
| CN113564081B CN113564081B (en) | 2023-04-18 |
Family
ID=78169662
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110875403.0A Active CN113564081B (en) | 2021-07-30 | 2021-07-30 | Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113564081B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114517231A (en) * | 2022-03-03 | 2022-05-20 | 山东第一医科大学(山东省医学科学院) | Specific primer, detection method and kit for Eggerthella lenta |
| KR20230096372A (en) * | 2021-12-23 | 2023-06-30 | 국립낙동강생물자원관 | Mixed strain having excellent abillity of removing recalcitrant organic compounds, and methodnd for treating wastewater using the same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387950A (en) * | 2013-08-06 | 2013-11-13 | 国家粮食局科学研究院 | Devosia sp and application thereof in degrading vomitoxin |
| CN104232517A (en) * | 2014-08-22 | 2014-12-24 | 中国农业大学 | Trichothecene mycotoxin biodegradation agent and preparation method thereof |
| CN112126607A (en) * | 2020-11-26 | 2020-12-25 | 中国农业科学院北京畜牧兽医研究所 | Bacterial strain for degrading deoxynivalenol and application |
-
2021
- 2021-07-30 CN CN202110875403.0A patent/CN113564081B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103387950A (en) * | 2013-08-06 | 2013-11-13 | 国家粮食局科学研究院 | Devosia sp and application thereof in degrading vomitoxin |
| CN104232517A (en) * | 2014-08-22 | 2014-12-24 | 中国农业大学 | Trichothecene mycotoxin biodegradation agent and preparation method thereof |
| CN112126607A (en) * | 2020-11-26 | 2020-12-25 | 中国农业科学院北京畜牧兽医研究所 | Bacterial strain for degrading deoxynivalenol and application |
Non-Patent Citations (5)
| Title |
|---|
| MING LIU等: "Pseudomonas xinjiangensis sp. nov., a moderately thermotolerant bacterium isolated from desert sand", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 * |
| YU PANG 等: "Devosia salina sp. nov., isolated from South China Sea sediment", 《INT. J. SYST. EVOL. MICROBIOL》 * |
| 李晓凤等: "生物降解呕吐毒素的肠杆菌筛选与鉴定", 《现代食品科技》 * |
| 李笑樱: "降解呕吐毒素德沃斯氏菌的饲用安全性和有效性评价", 《中国博士学位论文电子期刊全文数据库》 * |
| 汪洋等: "脱氧雪腐镰刀菌烯醇生物脱毒的研究进展", 《中国粮油学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230096372A (en) * | 2021-12-23 | 2023-06-30 | 국립낙동강생물자원관 | Mixed strain having excellent abillity of removing recalcitrant organic compounds, and methodnd for treating wastewater using the same |
| KR102671162B1 (en) | 2021-12-23 | 2024-05-31 | 국립낙동강생물자원관 | Mixed strain having excellent abillity of removing recalcitrant organic compounds, and methodnd for treating wastewater using the same |
| CN114517231A (en) * | 2022-03-03 | 2022-05-20 | 山东第一医科大学(山东省医学科学院) | Specific primer, detection method and kit for Eggerthella lenta |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113564081B (en) | 2023-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109576187B (en) | Cyanide degradation strain and method for degrading cyanide by using same | |
| CN111254093A (en) | Bacillus belgii 229-15 and application thereof | |
| CN113564081B (en) | Devorax SCS-3 for producing vomitoxin degrading enzyme and application thereof | |
| CN110904004B (en) | Bacterium for producing trehalose hydrolase and breeding method and application thereof | |
| CN111269858A (en) | Stenotrophomonas species and uses thereof | |
| CN106834177A (en) | One plant of cud bacterium and its application | |
| CN112574918B (en) | Ammonia nitrogen degrading bacteria, microbial agent and application thereof | |
| KR101475589B1 (en) | A novel microorganism Rhodococcus pyridinovorans EDB2 degrading aromatic compounds | |
| CN107164280B (en) | Vomitoxin degrading bacterium and application thereof | |
| CN113717886B (en) | Bacillus coagulans and method for producing 2' -deoxyadenosine by catalysis thereof | |
| CN113980852B (en) | Microbial composition for synergistic degradation of benzonitrile herbicide and microbial agent produced by same | |
| CN113061550B (en) | Lactobacillus new strain Z6 and application thereof in food | |
| CN112322539B (en) | Enterococcus faecium R-NTR-1 from ocean and screening method and application thereof | |
| CN105670965B (en) | Strain with iron reduction capacity and application thereof | |
| CN110343636B (en) | Stachys strain and application thereof in zearalenone degradation | |
| CN113308395B (en) | Shen's bacillus Z-25 for producing mycotoxin zearalenone degrading enzyme and application thereof | |
| CN111647537A (en) | Salt-tolerant capsaicin degrading bacteria, application and kitchen waste treatment method | |
| CN110616177A (en) | Bacillus with high fermentation density and fermentation production method thereof | |
| CN113897305B (en) | Isowa and application thereof | |
| CN113564066B (en) | Pseudomonas south China sea for producing lipase and application thereof | |
| CN117721038B (en) | Biocontrol strain E16 and application thereof in preventing and controlling aspergillus flavus | |
| CN108441437A (en) | A kind of composite bacteria agent and its application | |
| CN114774330B (en) | Novel species of cellulolytic female phytes and application thereof | |
| CN113249272B (en) | Novel marine-source halotolerant zoogloea forming strain and application thereof | |
| CN114657097B (en) | Bacillus belgii LGT-1 capable of efficiently antagonizing ralstonia solanacearum and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |