CN103387950A - Devosia sp and application thereof in degrading vomitoxin - Google Patents
Devosia sp and application thereof in degrading vomitoxin Download PDFInfo
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Abstract
The invention discloses devosia sp. and application thereof in degrading vomitoxin. The strain provided by the invention belongs to a new strain in the Devosia Nakagawa et al, and the preservation number of Devosia sp. DDB001 is CGMCC (China General Microbiological Culture Collection Center) No.7185. The invention also provides a fungicide containing the preserved strain and a preparation method thereof. Furthermore, the invention also provides an application of the strain or fungicide in degrading vomitoxin.
Description
Technical field
The present invention relates to a strain De Wosi Salmonella and the application in the degraded vomitoxin thereof.
Background technology
Deoxynivalenol (deoxynivalnol, DON), chemical name is 3 α, 7 α, 15-trihydroxy--12, the single-ended spore of 13-epoxy is mould-9-alkene-8-ketone, it is mainly to infect by Fusarium graminearum (Fusarium graminearum), fusarium culmorum (Fusarium culmorum) the Trichothecenes toxin that the cereal such as wheat, barley, oat, corn produce, because this material can cause the symptoms of emesis of animal, therefore have another name called vomitoxin (vomitoxin).In worldwide, DON is one of main pollution mycotoxin of grain, feed, food, has a strong impact on the health of people and livestock.After people and animals have taken in the food that is polluted by vomitoxin, can cause apocleisis, vomiting, diarrhoea, fever, astasia, the acute poisoning symptom such as slow in reacting, when serious, the infringement hemopoietic system causes death, and its serious harm has caused the generally attention of various countries.
Vomitoxin is quite general to the pollution of China's cereals raw material, its recall rate and detected level are all the highest a kind of in mycotoxin, investigate demonstration according to people such as Zhen Yangguang, the ratio that exceeds standard of China's feed and raw material vomitoxin is near 70%, in corn, the exceeding standard rate of vomitoxin is 57.1%, the toxin average content is 1.01mg/kg, and its high-content is 2.13mg/kg.Universal existence due to vomitoxin in cereal, feed, high-content characteristic and acute toxicity and chronic toxicity, reduce or remove its toxicity and seem particularly important and urgent.At present, the vomitoxin poison-removing method mainly contains Physical, chemical treatment and biological method three major types both at home and abroad.Although physics, chemical process detoxification have obtained success to a certain degree, but still there are the shortcomings such as detoxification efficiency loss, cost limited, that may cause important nutrient are higher.Biological method has the virulence that can make toxin under the condition of gentleness to be reduced, and on advantages such as the impacts such as the sensory properties of raw material, palatability are minimum, and be considered to best poison-removing method.Because microbe species is many, wide material sources, nearly all organic pollutant can be decomposed by microorganism, and has degraded thoroughly, and the advantage of non-secondary pollution, so the microbial detoxification of vomitoxin is a kind of effective poison-removing method.Along with the drawback of physics, chemical detoxication means constantly occurs and the understanding of the biotechnology advantage of fast development is increased, utilizing microorganism to carry out detoxicated research both at home and abroad progressively launches, although relevant achievement in research with microbial detoxification is also few, on going result has all showed good development and application prospect.the people such as Yoko in 2011 screen a strain promise Ka Shi and belong to bacterial strain in soil, this bacterial strain can utilize vomitoxin to be sole carbon source and energy growth under aerobic condition, its degraded toxin concentration scope is 10 – 1000 μ g/mL(Nocardioides sp.strain WSN05-2, isolated from a wheat field, degrades deoxynivalenol, producing the novel intermediate 3-epideoxynivalenol, Ikunaga Y, Sato I, Grond S, et al. (2011), Appl Microbiol Biotechnol89:419 – 427).
In sum, for solving toxin pollution problem in feed, feedstuff raw material, be necessary that from natural resources separation screening is efficient, the microorganism strains of safe disposal vomitoxin, further study its biological nature, toxin degradation characteristic and mechanism of degradation, research and development are applicable to the mycotoxin microbial detoxification agent of feedstuff industry, improve the grain utilization ratio, guarantee the safety in production of livestock industry, improve the animal husbandry economy benefit.
Summary of the invention
The technical problem to be solved in the present invention is to provide a strain can degrade De Wosi Salmonella and the application thereof of vomitoxin.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
The invention provides a strain De Wosi Salmonella (Devosia sp.), called after DDB001, belong to the novel species in the De Wosishi genus, to separate and obtain from the soil of Nanyang Prefecture plantation wheat, (be called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica on January 22nd, 2013, to be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, postcode 100101), its deposit number is CGMCC No.7185.The same with other biological situation, the bacterial strain DDB001 that the present invention has the degraded vomitoxin still easily morphs.Therefore, can utilize physics and chemistry method known in the art to obtain the mutant strain of this bacterial strain.For example, can be by with chemical agent such as N-methyl-N'-nitro-N-nitrosoguanidine, processing and obtain its mutant strain, these mutagenic mutants,, as long as kept the such feature of vomitoxin degradation capability, also belong to a part of the present invention.
The present invention also provides a kind of microbial inoculum, and its activeconstituents is De Wosi Salmonella (Devosia sp.) DDB001, and its deposit number is CGMCC No.7185.This microbial inoculum can be that the liquid-type microbial inoculum can be also the solid type microbial inoculum, and by published preparation method in prior art, prepares.Particularly, the invention provides a kind of preparation method of above-mentioned microbial inoculum, the method comprises:
(1) be that De Wosi Salmonella (Devosia sp.) DDB001 of CGMCC No.7185 activates with deposit number on solid medium;
(2) bacterial classification after activating is inoculated in seed culture medium and is cultured to logarithmic phase, makes seed liquor;
(3) described seed liquor is inoculated in fermention medium and is cultured to stationary phase, make the liquid-type microbial inoculum.
Further, the method also comprises mixes aforesaid liquid type microbial inoculum with sorbent material, make the solid type microbial inoculum after drying.Wherein, the weight ratio of described liquid-type microbial inoculum and sorbent material is 1:2 – 10, and described sorbent material can be one or more in corn cob meal, wheat bran, starch, diatomite, vermiculite, light calcium carbonate or peat.
Further, in above-mentioned preparation method, the substratum that is used for cultivating De Wosi Salmonella culture is diversified, but economy, cellular biomass, detoxification active angle, preferably some substratum from producing.For example, preferred carbon source is glucose, but also can use maltose, glycerine, fructose, semi-lactosi, seminose, alpha-lactose etc.Preferred nitrogenous source is yeast extract paste, casein peptone, but also can use corn steep liquor, yeast extract paste, extractum carnis etc.The nutrition inorganic salt that can mix in substratum have the conventional soluble salt that can produce following ion: zine ion, sodium ion, magnesium ion, calcium ion, iron ion, chlorion, carbanion, sulfate ion, nitrate ion etc.In an embodiment of the invention, described seed culture medium is comprised of according to volume ratio following component: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, sodium-chlor 0.4%, PH7.2 – 7.4; Described fermention medium is comprised of according to volume ratio following component: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, NaCl0.4%, K
2HPO
43H
2O0.3%, KH
2PO
40.075g/L, PH7.2 – 7.4.
Further, in above-mentioned preparation method, the bacterial classification after described activation is inoculated into seeding tank by 1 – 10% inoculum size of seed culture medium in seeding tank; Described seed liquor is inoculated into fermentor tank by 1 – 10% inoculum size of fermentation cylinder for fermentation substratum.
Further, step (3) fermentation culture conditions is: the air flow of sterile air is 1:0.5 – 1.2, and stirring velocity is 240 rev/mins of 80 –, and culture temperature is 37 ℃ of 28 – approximately, and in the liquid-type microbial inoculum, viable cell quantity reaches 10 at least
9More than CFU/ml.
The present invention also provides described moral Butterworth Salmonella (Devosia sp.) DDB001 or the application of its microbial inoculum in the degraded vomitoxin.
Particularly, in degraded during vomitoxin, adopt following (a) or (b) any in mode:
(a). the liquid-type microbial inoculum is sprayed in the cereal for the treatment of detoxification or feed and feedstuff raw material, the degraded vomitoxin, the viable cell quantity of described liquid-type microbial inoculum reaches 10 at least
7CFU/ml;
(b). the addition that is 0.01 – 5% according to the quality percentage composition with the solid type microbial inoculum joins in the cereal or feedstuff raw material and feed for the treatment of detoxification, mixes the degraded vomitoxin.
In above-mentioned application, described cereal is corn, wheat, barley, paddy or jowar.Described feedstuff raw material and feed are the feed that the feedstuff raw materials such as corn, wheat, barley, paddy or jowar and they process.
The invention has the advantages that: preserving number provided by the invention is that the De Wosi Salmonella strain of CGMCC No.7185 has good degradation effect to vomitoxin.Use solid type or the liquid-type microbial preparation of preserving number as the bacterial strain production of CGMCC No.7185 degraded vomitoxin, have a production and application cost low, simple, easy to operate, the advantage such as the vomitoxin degraded is safe, efficient.Bacterial strain provided by the invention and microbial inoculum can be used for the removal of cereal, feed, feedstuff raw material vomitoxin, for solving toxin pollution problem in feed and raw material thereof, improve the grain utilization ratio, guarantee that the safety in production of livestock industry, raising animal husbandry economy benefit have great importance.
Description of drawings
The electromicroscopic photograph of Fig. 1: DDB001 (* 15,000);
Fig. 2: the DDB001 that the employing method of closing on builds based on 16S rRNA gene order and the phylogenetic evolution tree of close bacterial strain;
Fig. 3: DDB001 is toxin degradation effect in the TY of 200 μ g/mL in vomitoxin concentration;
Fig. 4: DDB001 liquid-type microbial inoculum and the solid type microbial inoculum degradation effect to vomitoxin in cereal.
Embodiment
The present invention will be further described below in conjunction with specific embodiment, but be not to limit the present invention.Experimental technique in following embodiment, if no special instructions, be ordinary method.Test materials used in following embodiment, if no special instructions, be the routine biochemistry reagent suppliers and buy and obtain.
Embodiment 1 moral Butterworth Salmonella (Devosia sp.) DDB001 obtains and identifies
gather soil sample in Nanyang, henan wheat field, take 96 microwell plates as culture carrier, at first pass through the enrichment culture method of 6 continuous toxin concentration gradients, acquisition has the bacteria suspension of degraded vomitoxin, then bacteria suspension is coated on TY agar plate (yeast extract 4g/L by method of dilution butteron on plate with suitable extent of dilution, Tryptones 8g/L, agar powder 15g/L, pH7.2, 121 ℃ of sterilizing 30min) on, good and the colonial morphology of picking separation degree, the bacterium colony that color is different, be TY liquid nutrient medium (the yeast extract 4g/L of 200 μ g/mL in DON concentration, Tryptones 8g/L, pH7.2, 121 ℃ of sterilizing 30min) carry out the detoxification test in, the residual toxin of ethyl acetate extraction also carries out HPLC and detects, verify the toxin degradation effect of each pure growth, the bacterial strain DDB001 of the final vomitoxin that successfully obtains to degrade, the single bacterium colony of picking DDB001 is in the TY liquid nutrient medium, be cultured to logarithmic phase during mid-term, glycerine with 50% mixes with the culture equal-volume and is placed on-80 ℃ of preservations.
Adopt the classification such as 16S rRNA sequential analysis, Physiology and biochemistry, Chemical Characteristics to the degradation bacteria strains evaluation of classifying, result shows that the classification position of this bacterial strain belongs to De Wosishi in Hyphomicrobiaceae (Hyphomicrobiaceae) and belongs to novel species member in (Devosia), and the Genbank accession number of its 16S rRNA sequence is JX392051.On January 22nd, 2013 Devsoia sp.DDB001 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center and (is called for short CGMCC, address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), its deposit number is CGMCC No.7185.
Cultural characteristic and morphological specificity: this bacterial strain is on the TY solid medium, at 30 ℃ of temperature, through its colonial morphology of the cultivations of 3 days: ivory white, circle and neat in edge, smooth surface and projection, big or small 0.5 – 1.5 μ m; Microscopic morphology: bar-shaped, big or small 0.7 – 1.3 μ m * 0.5 – 0.8 μ m, single polar flagella (as shown in Figure 1), without sporulation, Gram-negative, strictly aerobic.
Physiological characteristic: catalase and oxydase all are positive, and can grow in salt or salt-free TY substratum are arranged, and still can grow at the TY substratum that contains 3% sodium-chlor, but have no the growth phenomenon under 5% concentration; The pH tolerance range is 5.0 – 9.0, has surveyed 40 ℃ of growth temperature range 15 –.The carbon source that can utilize has L-arabinose, D-cellobiose, L-trehalose, D-semi-lactosi, alpha-D-glucose, maltose, D-MANNOSE, D-melibiose, gluconic acid, α-hydroxybutyric acid, D, Pfansteihl, D-Glucose diacid, N-ACETYL-D-GLUCOSAMINE, ribitol, gentiobiose, α-D-lactose, L-rhamnosyl, D-glucitol, sucrose, D-trehalose, turanose, Xylitol, monomethyl succinate, glucuronamide, glycerine, D-Glucose-6-phosphoric acid, D-R alcohol, D-Fructose, PEARLITOL 25C, lactulose; Unavailable carbon source has dextrin, acetic acid, Serine, D-Ser, L-PROLINE, L-Leu, raffinose, N-acetyl-D-galactosamine, cis equisetic acid, D-Glucosaminic acid, D-Glucose aldehydic acid.
16S rRNA sequential analysis: utilize primers F 9 (5'-GAGTTTGATCCTGGCTCAG-3') and R1544 (5'-AGAAAGGAGGTGATCCA-3') to carry out pcr amplification, length is that in the 16SrRNA sequence of 1479bp and Genbank database, existing sequence is carried out Blast and compared, it is higher that this sequence and Devosia belong to 16S rRNA similarity, and the similarity scope is between 94.6 – 96.0%.Belong in 16 all kinds of generally acknowledging at De Wosishi, similarity is the highest is Devosia insulae DS-56
TPhylogenetic tree shows (Fig. 2), DDB001 by cluster in this group of Devosia, DDB001 and Devosia insulae DS-56
T(EF012357) be in a branch, but their 16S rRNA sequence similarity only reaches 96%(＜97%), can not be attributed to same kind.
The main fatty acid of chemical constitution: DDB001 comprises 11-methyl ω 7c 18 carbon monounsaturated fatty acids (28.16%), saturated 16 carbon fatty acids (21.72%), ω 8c type ring type 19 saturated fatty acids (16.69%) and saturated 18 carbon fatty acids (14.95%) (in Table 1); Breathing the quinone main Types is Coenzyme Q10 99.0; The polar lipid main Types is phosphatidyl glycerol (PG) and diphosphatidylglycerol (DPG); G+C mol% is 64.6%.
Table 1
Show according to above all results, DDB001 is different from the typical strain of 16 kinds of generally acknowledging in the past, be enough to make it to become the novel species of De Wosishi in belonging to, the retrieval that this classification is based on direct laboratory relatively and the description of the similar species delivered is done.
The detoxification efficiency of embodiment 2 moral Butterworth Salmonella (Devosia sp.) DDB001 in the TY substratum
DDB001 after activation is seeded in the TY liquid nutrient medium of the 50mL that contains 200 μ g/mL vomitoxins with 1% inoculum size, the detoxification of carrying out under the oscillating condition of 200 rev/mins two days is cultivated, the remaining vomitoxin of ethyl acetate extracting, in final cultures, the residue content of vomitoxin shows through the HPLC detected result: the vomitoxin in TY is degradable, degradation rate reaches 100%, and the generation retention time is about the toxin metabolite (as shown in Figure 3) of 9min.
The preparation of embodiment 3 moral Butterworth Salmonella (Devosia sp.) DDB001 liquid-type microbial inoculums
Solid medium component and proportioning: yeast extract paste 0.8%, peptone 0.4%, NaCl0.5%, agar powder 1.5%, 7.4,121 ℃ of lower high-temp steam sterilizing 30min of pH7.2 –.
Seed culture medium component and proportioning: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, sodium-chlor 0.5%, pH7.2 – 7.4; Fermention medium component and proportioning: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, NaCl0.5%, K
2HPO
43H
2O0.3%, KH
2PO
40.075g/L, pH7.2 – 7.4.
(1) actication of culture: the De Wosi Salmonella DDB001 that is CGMCC No.7185 with preserving number is inoculated on solid medium, cultivates 3 days under 30 ℃ of conditions, and measures its toxin degradation property, is inoculated on test tube slant standby.
(2) seed culture: picking list bacterium colony access seed culture medium, be cultured to logarithmic phase under 30 ℃ of conditions from slant medium, make bacterial classification; Then, seeding tank with 500 liters, 350 liters of seed culture medium charging capacitys, the complete rear 121 ℃ of high pressure moist heat sterilizations that feed intake, after being cooled to 30 ℃, with above-mentioned cultured bacterial classification with the 10%(volume percent) inoculum size inoculate into seeding tank, stirring velocity is 200 rev/mins, and 30 ℃ of culture temperature, sterile air intake are the 1:0.8(volume ratio), approximately 48 – was cultured to logarithmic phase in 60 hours, obtained seed liquor.
(3) fermentation culture: the employing volume is the production tank of 5000 liters, and 3500 liters of fermention medium charging capacitys, at 1.1kg/cm
2Pressure, the temperature of 121 ℃ under, carry out the high pressure moist heat sterilization, sterilizing rear cooling 30 ℃, seed liquor is inoculated into fermentor tank by 10% inoculum size, fermentation condition: the air flow of sterile air is 1:0.8 – 1.2, and stirring velocity is 240 rev/mins, 30 ℃ of culture temperature, incubation time is 48 – 60 hours approximately, forms the liquid-type microbial inoculum with vomitoxin degraded after putting tank.In this liquid-type microbial inoculum, viable cell quantity reaches 10 at least
9More than CFU/ml.
The preparation of embodiment 4 moral Butterworth Salmonella (Devosia sp.) DDB001 solid type microbial inoculums
After the DDB001 liquid-type microbial inoculum that embodiment 3 is produced and corn cob meal mixed according to the weight ratio of 1:4, cryodrying below 40 ℃ to moisture was below 8%, forms solid state, thereby makes the solid type microbial inoculum of DDB001.
The degradation effect of embodiment 5 moral Butterworth Salmonella (Devosia sp.) DDB001 liquid-type microbial inoculums to vomitoxin in cereal
The DDB001 liquid-type microbial inoculum of embodiment 3 preparations is first diluted, and the liquid-type bacterial strain viable cell quantity after dilution reaches 10 at least
7Individual/ml, ratio by weight 1:1 is sprayed onto in the Semen Maydis powder that pollutes DON as test group, be sprayed onto with same ratio without bacteria fermentation culture medium in the Semen Maydis powder that pollutes toxin as a control group, every group of three repetitions, after mixing in detoxification at the temperature of 30 ℃ after 3 days, respectively from control group and the accurate weighing 5g of test group sample in centrifuge tube, add the acetonitrile of 20ml85% to the detoxification sample, vibration makes the abundant extracting of toxin, 4000 rev/mins, centrifugal 15 minutes, supernatant was transferred in new centrifuge tube; 85% acetonitrile solution that again adds 12ml, vibration mixes extracting again, similarity condition is centrifugal, merging supernatant spontaneous evaporation to acetonitrile volatilizees fully, add the saturated sodium-chloride of 12ml and mix, then use twice of the ethyl acetate extracting of 12ml, after merging twice ethyl acetate and naturally volatilizing mutually, the 1ml methanol constant volume is dissolved, detect the content of vomitoxin with high performance liquid chromatography, result shows DDB001 liquid-type microbial inoculum to the detoxification of vomitoxin pollution Semen Maydis powder through 3 days, and its degradation rate can reach 92.4%, and control group is without any signs of degradation (the results are shown in Figure 4).
The degradation effect of embodiment 6 moral Butterworth Salmonella (Devosia sp.) DDB001 solid type microbial inoculums to vomitoxin in cereal
add to the solid type microbial inoculum of embodiment 4 preparations in the test group Semen Maydis powder that pollutes vomitoxin according to 4% weight ratio, fermention medium and corn cob meal (with the 1:4 ratio, mixing) mixture joins in the control group Semen Maydis powder that pollutes vomitoxin with 4% weight ratio equally, control group and test group are all added distilled water in the ratio of 1:1, every group of three repetitions, detoxification 3 days at the temperature of 30 ℃ after mixing, put into the 50ml centrifuge tube from control group and the accurate weighing 5g of test group sample respectively, add the acetonitrile of 20ml85% to sample, vibration makes the abundant extracting of toxin, 4000 rev/mins, centrifugal 15 minutes, supernatant is transferred in new centrifuge tube, solid substance adds 85% acetonitrile solution of 12ml again, vibration mixes extracting again, centrifugal under similarity condition, merging supernatant spontaneous evaporation to acetonitrile volatilizees fully, add the saturated sodium-chloride of 12ml and mix, then use twice of the ethyl acetate extracting of 12ml, after merging twice ethyl acetate and naturally volatilizing mutually, the 1ml methanol constant volume is dissolved, detect the content of vomitoxin with high performance liquid chromatography, result shows DDB001 solid type microbial inoculum to the detoxification of pollution Semen Maydis powder through 3 days, and its degradation rate can reach 84.9%, and control group is without any signs of degradation (the results are shown in Figure 4).
Obviously, the above embodiment of the present invention is only for example of the present invention clearly is described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here can't give all embodiments exhaustive.Everyly belong to the row that apparent variation that technical scheme of the present invention extends out or change still are in protection scope of the present invention.
Claims (10)
1. strain De Wosi Salmonella (Devosia sp.) DDB001, its deposit number is CGMCC No.7185.
2. moral Butterworth Salmonella according to claim 1 (Devosia sp.) DDB001, is characterized in that, the accession number of the 16SrRNA of this bacterial strain on GenBank is JX392051.
3. microbial inoculum, its activeconstituents is De Wosi Salmonella (Devosia sp.) DDB001, its deposit number is CGMCC No.7185.
4. microbial inoculum according to claim 3, is characterized in that, this microbial inoculum is liquid-type microbial inoculum or solid type microbial inoculum.
5. the preparation method of a microbial inoculum claimed in claim 3, is characterized in that, the method comprises:
(1) be that De Wosi Salmonella (Devosia sp.) DDB001 of CGMCC No.7185 activates with deposit number on solid medium;
(2) bacterial classification after activating is inoculated in seed culture medium and is cultured to logarithmic phase, makes seed liquor;
(3) described seed liquor is inoculated in fermention medium and is cultured to stationary phase, make the liquid-type microbial inoculum.
6. the preparation method of microbial inoculum according to claim 5, is characterized in that, the method also comprises mixes the liquid-type microbial inoculum with sorbent material, make the solid type microbial inoculum after drying.
7. the preparation method of microbial inoculum according to claim 5, is characterized in that, described seed culture medium is comprised of according to volume ratio following component: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, sodium-chlor 0.4%, pH7.2 – 7.4; Described fermention medium is comprised of according to volume ratio following component: yeast extract paste 0.8%, peptone 0.4%, glucose 0.2%, NaCl0.4%, K
2HPO
43H
2O0.3%, KH
2PO
40.075g/L, pH7.2 – 7.4; Further, step (3) fermentation culture conditions is: the air flow of sterile air is 1:0.5 – 1.2, and stirring velocity is 240 rev/mins of 80 –, and culture temperature is 37 ℃ of 28 – approximately, and in the liquid-type microbial inoculum, viable cell quantity reaches 10 at least
9More than CFU/ml.
8. the preparation method of microbial inoculum according to claim 6, it is characterized in that, described liquid-type microbial inoculum mixes by the weight ratio of 1:2 – 10 with sorbent material, wherein, described sorbent material is one or more in corn cob meal, wheat bran, starch, diatomite, vermiculite, light calcium carbonate, peat.
9. the described moral Butterworth of claim 1 Salmonella (Devosia sp.) DDB001 or the described microbial inoculum of claim 3 – 4 application in the degraded vomitoxin.
10. application according to claim 9, is characterized in that, in degraded during vomitoxin, adopts following (a) or (b) any in mode:
(a). the liquid-type microbial inoculum is sprayed in the cereal for the treatment of detoxification or feed and feedstuff raw material, the degraded vomitoxin, the viable cell quantity of described liquid-type microbial inoculum reaches 10 at least
7CFU/ml;
(b). the addition that is 0.01 – 5% according to the quality percentage composition with the solid type microbial inoculum joins in the cereal or feedstuff raw material and feed for the treatment of detoxification, mixes the degraded vomitoxin.
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