CN102008401A - Biological preparation for relaxing facial muscles and preparation method thereof - Google Patents
Biological preparation for relaxing facial muscles and preparation method thereof Download PDFInfo
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- CN102008401A CN102008401A CN 201010556446 CN201010556446A CN102008401A CN 102008401 A CN102008401 A CN 102008401A CN 201010556446 CN201010556446 CN 201010556446 CN 201010556446 A CN201010556446 A CN 201010556446A CN 102008401 A CN102008401 A CN 102008401A
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- biological preparation
- structural protein
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- facial muscle
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Abstract
The invention discloses a biological preparation for relaxing facial muscles, and belongs to a human skin health product. The invention aims to prepare the biological preparation for beautifying the facial muscles from simple and low-cost raw materials. The biological preparation is a structural protein capable of inducing to generate glycoprotein, and the structural protein contains a peptide chain consisting of Ser-Hyp-Hyp-Hyp-Hyp pentapeptide. The product can relax the facial muscles, promote the generation of cell walls and improve the surface activity of the skin, has the effects of firming the skin, reducing wrinkles, and moistening and smoothening the skin, and has obvious conditioning effect on the skin.
Description
Technical field
A kind of biological preparation and manufacture method thereof of loosening facial muscle belongs to the human skin health product, can promote the generation of facial muscle cell wall, strengthens the skin surface activity.
Background technology
Used beauty and skin care product is a lot, and still, the component of goods is more, makes complicated.As: name is called the patent of " a kind of skin-protecting and beautifying liquid " (02135043.3), and its composition comprises plurality of raw materials such as water-soluble pearl protein liquid, water solublity Aloe, and its extracting solution technology is more.
Summary of the invention
A kind of biological preparation and manufacture method thereof of loosening facial muscle that the present invention proposes its objective is the biological preparation of wanting the raw material manufacturing beauty treatment facial muscle that materials are simple, cost is low.
A kind of biological preparation that loosens facial muscle is a kind of structural protein that produce glycoprotein of inducing, and contains the peptide chain of the pentapeptide formation of Ser-Hyp-Hyp-Hyp-Hyp in this structural protein.
Above-mentioned structural protein content is: 1mg-10mg.
Above-mentioned a kind of described structural protein of biological preparation that loosen facial muscle are structural protein of a kind of plant primary cell wall.
Above-mentioned a kind of biological preparation that loosens facial muscle can be made into: the water liquid of wrinkle of skin; The wrinkle of skin gel; Wrinkle of skin frost (breast) agent.
Through check, the Radix Dauci Sativae root contains the proteic peptide chain of said structure.
Above-mentioned a kind of manufacture method of loosening the biological preparation of facial muscle is;
In GMP standard workshop, in the container of cleaning, add Radix Dauci Sativae undercut sheet, containing 2, induce the generation callus on the MS culture medium of 4-D (1mg/L) kinetins (0.1mg/L), then under freezing (16 ℃) condition, the adding pH value is 6.0 potassium dihydrogen phosphate buffer solution, smash to pieces in tissue mashing machine's high speed (per minute reaches 12000 to be changeed), centrifugal branch removes supernatant, the residue precipitate does not extremely contain till the albumen with same buffer solution washing, through the cell wall of thorough washing precipitation is the calcium chloride solution extraction 12 hours of 0.2mol/L (4 ℃) with freezing concentration, then, remove precipitate with centrifuge, the supernatant of obtaining promptly gets these goods through ultrafiltration (implementor name hollow fiber ultrafiltration membrane machine).
Effect of the present invention: loosen facial muscle, promote the generation of cell wall, strengthen the skin surface activity, Firm is arranged and reduce the wrinkle effect, have moistening and soft and smooth skin effect simultaneously, skin is had remarkable conditioning effect.
The specific embodiment
With several example explanation the present invention.
Embodiment one:
After being mixed by a kind of structural protein 10g of Radix Dauci Sativae root primary cell wall and Purified Water 90ml is that 100ml makes the water liquid that external reduces wrinkle of skin.
Embodiment two:
Adding 5g card ripple 941 after being mixed by a kind of structural protein 10g of Radix Dauci Sativae root primary cell wall and Purified Water 85ml makes 100 gram externals and reduces the wrinkle of skin gels.
Embodiment three:
Structural protein 10g and Purified Water 85 mixing by a kind of Radix Dauci Sativae root primary cell wall are made 100 gram externals minimizing wrinkle of skin frost (breast) agent through the emulsifying of 5g emulsifying agent.
The clinical experiment data;
Zhang Yan; Sex; The man, the age; 40 years old;
The readme face skin wrinkle increases.Through loosening biological preparation 2 each 1 grams every day of facial muscle, treat 24 all skin lines and alleviate 50%.
Li Na; Sex; The woman; Age; 38 years old;
The readme eye wrinkle-removing is deepened.Biological preparation treatment 2 each 0.3 gram 16 week treatment skin lines every day through loosening facial muscle alleviate 50%.
Claims (6)
1. a biological preparation that loosens facial muscle is a kind of structural protein that produce glycoprotein of inducing, and contains the peptide chain of the pentapeptide formation of Ser-Hyp-Hyp-Hyp-Hyp in this structural protein.
2. according to the described a kind of biological preparation that loosens facial muscle of claim 1, it is characterized in that described structural protein content is: 1mg-10mg.
3. according to claim 1 or 2 described a kind of biological preparation that loosen facial muscle, it is characterized in that described structural protein are structural protein of a kind of plant primary cell wall.
4. according to the described a kind of biological preparation that loosens facial muscle of claim 1, it is characterized in that this biological preparation can be made into the water liquid of wrinkle of skin, wrinkle of skin gel and wrinkle of skin frost (breast) agent.
5. according to the described a kind of biological preparation that loosens facial muscle of claim 3, the structural protein that it is characterized in that a described kind of plant primary cell wall are structural protein that the Radix Dauci Sativae root contains.
6. according to claim 1 or 2 described a kind of biological preparation that loosen facial muscle, its manufacture method is;
In GMP standard workshop, in the container of cleaning, add Radix Dauci Sativae undercut sheet, containing 2, induce the generation callus on the MS culture medium of 4-D (1mg/L) kinetins (0.1mg/L), freezing under-16 ℃ temperature conditions then, the adding pH value is 6.0 potassium dihydrogen phosphate buffer solution, in speed is that per minute reaches in 12000 high-speed tissue mashing machines that change and smashs to pieces, remove supernatant with the centrifuge branch then, the residue precipitate does not extremely contain till the albumen with same buffer solution washing, the concentration that precipitates freezing usefulness through the cell wall of thorough washing is that the calcium chloride solution of 0.2mol/L (4 ℃) extracted 12 hours, then, remove precipitate with centrifuge, the supernatant of obtaining promptly gets these goods through ultrafiltration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010556446 CN102008401A (en) | 2010-11-22 | 2010-11-22 | Biological preparation for relaxing facial muscles and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010556446 CN102008401A (en) | 2010-11-22 | 2010-11-22 | Biological preparation for relaxing facial muscles and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102008401A true CN102008401A (en) | 2011-04-13 |
Family
ID=43838878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010556446 Pending CN102008401A (en) | 2010-11-22 | 2010-11-22 | Biological preparation for relaxing facial muscles and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102008401A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0533408A2 (en) * | 1991-09-18 | 1993-03-24 | Revlon Consumer Products Corporation | Cosmetics and pharmaceuticals containing extensins |
| CN1171822A (en) * | 1994-12-28 | 1998-01-28 | 因迪纳有限公司 | Hydroxyproline-rich proteins and pharmaceutical and cosmetic formulations containing them |
| CN1491638A (en) * | 2002-10-23 | 2004-04-28 | 吴凤锷 | Skin-care cosmetic article for neck |
-
2010
- 2010-11-22 CN CN 201010556446 patent/CN102008401A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0533408A2 (en) * | 1991-09-18 | 1993-03-24 | Revlon Consumer Products Corporation | Cosmetics and pharmaceuticals containing extensins |
| CN1171822A (en) * | 1994-12-28 | 1998-01-28 | 因迪纳有限公司 | Hydroxyproline-rich proteins and pharmaceutical and cosmetic formulations containing them |
| CN1491638A (en) * | 2002-10-23 | 2004-04-28 | 吴凤锷 | Skin-care cosmetic article for neck |
Non-Patent Citations (1)
| Title |
|---|
| 《化妆品天然功能成分》 20070131 王建新 伸展蛋白 第33-34页 1-6 , 1 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20110413 |