CN101999538A - Composite immunity reinforcing agent for cyprinus carpio var. koi - Google Patents
Composite immunity reinforcing agent for cyprinus carpio var. koi Download PDFInfo
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- CN101999538A CN101999538A CN2010105276382A CN201010527638A CN101999538A CN 101999538 A CN101999538 A CN 101999538A CN 2010105276382 A CN2010105276382 A CN 2010105276382A CN 201010527638 A CN201010527638 A CN 201010527638A CN 101999538 A CN101999538 A CN 101999538A
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Abstract
The invention discloses a composite immunity reinforcing agent for cyprinus carpio var. koi. The reinforcing agent comprises the following components in percentage by mass: 35 to 45 percent of raffinose, 25 to 35 percent of low molecular chitosan oligosaccharide, 15 to 20 percent of glycyrrhetic acid and 10 to 15 percent of lactic acid bacillus. The reinforcing agent can obviously reinforce the immunity and the disease resistance of the cyprinus carpio var. koi and improve the survival rate by 12 to 19 percent; the immune effect of the composite immunity reinforcing agent is remarkably higher than that of a single immunity reinforcing agent, and the composite immunity reinforcing agent also can overcome immune suppression caused by long-term use of a single product; and a method for preparing the composite immunity reinforcing agent has the advantages of stable and low-cost raw material source and simple production process.
Description
Technical field
The present invention relates to a kind of fish feed additive, particularly relate to a kind of bright and beautiful carp and use composite immune reinforcing agent.
Background technology
Along with the development of recreational fisheries, bright and beautiful carp (
Cyprinus carpio var.koi) becoming one of popular and well received large-scale pet fish, its breed scale constantly enlarges, and the intensification degree improves constantly.At present China has become major country of production and the exported country of world brocade carp, and bright and beautiful carp is cultured optimizing and revising and increase foreign exchange earnings from exports and made significant contribution China's fishery industrial structure.Along with the change of bright and beautiful carp aquaculture model, also more and more higher to the requirement of man-made feeds preparation technique, and tradition is cultured the main preparation technique of paying close attention to the man-made feeds of body colour improvement, has not satisfied the needs of modern bright and beautiful carp aquaculture sustainable development.Especially in recent years, bright and beautiful carp is cultured the raising of intensification degree, and the trend of increase takes place also to have its disease, has a strong impact on its ornamental value and commodity value, so improve premunition, it is important that the generation of pre-disease prevention shows concerning bright and beautiful carp is cultured especially.
Though at present single immunopotentiator is arranged, does not still have the relevant research report that improves the composite immune reinforcing agent of bright and beautiful carp premunition.
Summary of the invention
The purpose of this invention is to provide a kind of bright and beautiful carp and use composite immune reinforcing agent, it can improve bright and beautiful carp premunition and survival rate effectively, increases added value of product, promotes to view and admire sustainable development of fishery.
A kind of bright and beautiful carp of the present invention is used composite immune reinforcing agent, and its component and quality percentage composition are as follows:
Raffinose 35～45%, low molecule chitosan oligosaccharide 25～35%, glycyrrhizic acid 15～20, lactic acid bacillus 10～15.
A kind of bright and beautiful carp of the present invention is simple with the preparation method of composite immune reinforcing agent: take by weighing each component, wear into powder respectively, cross 200 mesh sieves, in mixer, mix then, make the coefficient of variation≤5% of gained mixture, be bright and beautiful carp of the present invention and use composite immune reinforcing agent.
A kind of bright and beautiful carp of the present invention is that as bright and beautiful carp feed addictive use, addition is 0.1～0.2% of a feeding quality with the using method of composite immune reinforcing agent.
Bright and beautiful carp of the present invention has following advantage with composite immune reinforcing agent: (1) can obviously strengthen immunity and the premunition of bright and beautiful carp, improves survival rate 12～19%; (2) immune effect is significantly higher than single immunopotentiator (concrete data are seen embodiment), but also can overcome the long-term immunosupress phenomenon that is showed of using of single product, can use in the complete period of animal-breeding; (3) it is stable, cheap to prepare this composite immune reinforcing agent its raw material source, and production technology is simple.
The specific embodiment
Embodiment 1:
Take by weighing raffinose 40 kg, low molecule chitosan oligosaccharide 30 kg, glycyrrhizic acid 18 kg and bacillus subtilis 12 kg, wear into powder respectively, cross 200 mesh sieves, in mixer, mix then, make the coefficient of variation≤5% of gained mixture, be bright and beautiful carp of the present invention and use composite immune reinforcing agent.Carry out the breed of bright and beautiful carp respectively everywhere in the test site indoor circulation cultivating system in national zones of different, the initial weight of fish is respectively: 5.72 ± 0.36 g, 12.32 ± 0.78 g, 21.36 ± 1.15 g and 35.78 ± 2.18 g.All do not add bright and beautiful carp of the present invention in the feed that control group is used and use composite immune reinforcing agent, all add the 0.1%(mass percent during experimental group is feeds utilized) product of the present invention.Control group and experimental group are divided into 3 repetitions respectively.Behind 56 days raising experiment, the survival rate of the bright and beautiful carp of the experiment feed of feeding everywhere improves 18～24%, 15～21%, 12～19% and 10～14% respectively.And, main immune indexes phagocytic index, antalzyme activity and complement vigor (8.96%, 108.5 U ml in the bright and beautiful carp body of the experiment feed of feeding
-1With 115.75 U ml
-1) all be significantly higher than control group (4.35%, 66.78 U ml
-1With 83.62 U ml
-1).
Embodiment 2:
Take by weighing raffinose 35 kg, low molecule chitosan oligosaccharide 35 kg, glycyrrhizic acid 20 kg and bacillus subtilis 10 kg, wear into powder respectively, cross 200 mesh sieves, in mixer, mix then, make the coefficient of variation≤5% of gained mixture, be bright and beautiful carp of the present invention and use composite immune reinforcing agent.Carry out the land for growing field crops culture experiment at national zones of different two places respectively, the culture experiment of two place fishes and the experiment of control group are carried out on 3 mouthfuls of pools respectively, the average area on every mouthful of pool is 5 mu, does not add bright and beautiful carp of the present invention in the control group feed and uses composite immune reinforcing agent.Add 0.2% product of the present invention in the experiment feed.Behind 60 days raising experiment, the survival rate of fish all improves more than 12% than control group.And make 3 kinds of main immune indexes in the bright and beautiful carp body of breed, promptly phagocytic index, antalzyme activity and complement vigor all improve more than 1 times.
Embodiment 3:
Take by weighing raffinose 45 kg, low molecule chitosan oligosaccharide 25 kg, glycyrrhizic acid 15 kg and bacillus subtilis 15 kg, wear into fine powder respectively, cross 200 mesh sieves, in mixer, mix then, make the coefficient of variation≤5% of gained mixture, be bright and beautiful carp of the present invention and use composite immune reinforcing agent.Carry out bright and beautiful carp land for growing field crops culture experiment in zones of different, obtained the result similar to embodiment 1.
Claims (1)
1. a bright and beautiful carp is used composite immune reinforcing agent, it is characterized in that, its component and quality percentage composition are as follows:
Raffinose 35～45%, low molecule chitosan oligosaccharide 25～35%, glycyrrhizic acid 15～20, lactic acid bacillus 10～15.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010527638A CN101999538B (en) | 2010-11-02 | 2010-11-02 | Composite immunity reinforcing agent for cyprinus carpio var. koi |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010527638A CN101999538B (en) | 2010-11-02 | 2010-11-02 | Composite immunity reinforcing agent for cyprinus carpio var. koi |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101999538A true CN101999538A (en) | 2011-04-06 |
| CN101999538B CN101999538B (en) | 2012-10-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010527638A Expired - Fee Related CN101999538B (en) | 2010-11-02 | 2010-11-02 | Composite immunity reinforcing agent for cyprinus carpio var. koi |
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| CN (1) | CN101999538B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103211122A (en) * | 2013-04-23 | 2013-07-24 | 北京三友创美饲料科技有限公司 | Special coloring and brightening feed for fancy carp and preparation method feed |
| CN105707514A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Complex immunity enhancing agent for colossoma brachypomum fry breeding |
| CN106071418A (en) * | 2016-07-22 | 2016-11-09 | 天津市晨辉饲料有限公司 | Red white Cyprinus carpio L. build consolidates feedstuff and preparation method thereof |
| CN106616029A (en) * | 2016-12-08 | 2017-05-10 | 西南大学 | Feed additive capable of improving disease resistant performance of rainbow trout and preparation method thereof |
| CN108634127A (en) * | 2018-02-13 | 2018-10-12 | 河南科技大学 | A kind of fancy carp feed addictive and its preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390925A (en) * | 2008-10-16 | 2009-03-25 | 中国海洋大学 | Composite immunity strengthening agent for fresh water young fish |
| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
-
2010
- 2010-11-02 CN CN201010527638A patent/CN101999538B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390925A (en) * | 2008-10-16 | 2009-03-25 | 中国海洋大学 | Composite immunity strengthening agent for fresh water young fish |
| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
Non-Patent Citations (2)
| Title |
|---|
| 《中国食品添加剂》 20021231 袁美兰 功能性低聚糖-棉籽糖的开发应用现状 54-57 1 , 第4期 * |
| 《广东饲料》 19961231 梁思析 甘草酸-鱼类免疫增强剂 25 1 , 第3期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726606A (en) * | 2012-03-20 | 2012-10-17 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN102726606B (en) * | 2012-03-20 | 2013-11-20 | 重庆市畜牧科学院 | Micro-ecological synbiotic feed addictive, preparation method and application thereof |
| CN103211122A (en) * | 2013-04-23 | 2013-07-24 | 北京三友创美饲料科技有限公司 | Special coloring and brightening feed for fancy carp and preparation method feed |
| CN105707514A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Complex immunity enhancing agent for colossoma brachypomum fry breeding |
| CN106071418A (en) * | 2016-07-22 | 2016-11-09 | 天津市晨辉饲料有限公司 | Red white Cyprinus carpio L. build consolidates feedstuff and preparation method thereof |
| CN106616029A (en) * | 2016-12-08 | 2017-05-10 | 西南大学 | Feed additive capable of improving disease resistant performance of rainbow trout and preparation method thereof |
| CN106616029B (en) * | 2016-12-08 | 2020-05-01 | 西南大学 | Feed additive for improving disease resistance of rainbow trout and preparation method thereof |
| CN108634127A (en) * | 2018-02-13 | 2018-10-12 | 河南科技大学 | A kind of fancy carp feed addictive and its preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101999538B (en) | 2012-10-03 |
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