CN101986843A - Insect-resistant cotton phosphate extraction solution for controlling hyphantria cunea, production method and using method thereof - Google Patents
Insect-resistant cotton phosphate extraction solution for controlling hyphantria cunea, production method and using method thereof Download PDFInfo
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- CN101986843A CN101986843A CN2010102465753A CN201010246575A CN101986843A CN 101986843 A CN101986843 A CN 101986843A CN 2010102465753 A CN2010102465753 A CN 2010102465753A CN 201010246575 A CN201010246575 A CN 201010246575A CN 101986843 A CN101986843 A CN 101986843A
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Abstract
The invention relates to insect-resistant cotton phosphate extraction solution for controlling hyphantria cunea, a production method and a using method thereof, which belong to the technical field of pest control and aim at solving the problems of environmental pollution and potential threat to ecological safety caused by the existing control of the hyphantria cunea with pesticides, as well as slow reproduction and high cost caused by the control of the hyphantria cunea with natural enemies. The insect-resistant cotton phosphate extraction solution with the concentration of 1:9-12 is prepared by adopting insect-resistant cotton leaves, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and water, selecting insect-resistant cotton as raw material, preparing the insect-resistant cotton leaves, preparing phosphate buffer solution and preparing the insect-resistant cotton phosphate extraction solution, when in use, any one of the concentration of 1:15, 1:20, 1:25, 1:30, 1:40 and 1:50 is sprayed before moth emergence of the hyphantria cunea for controlling. The insect-resistant cotton phosphate extraction solution has obvious control effect, and can not only directly control the hyphantria cunea, but also be effectively interacted with high-efficient cypermethrin for preventing the hyphantria cunea from producing the drug resistance, thereby being applicable to controlling the hyphantria cunea and other pests.
Description
Technical field
The invention belongs to a kind of control fall webworm plant extraction liquid and making and use method thereof in the control of insect technical field.
Background technology
Fall webworm (Fall webworm) Hlyphantria cunea (Drury) belong to Lepidoptera, and Arctiidae is a kind of very harmful leaf-feeding insect, the serious threat ecological safety.Its harm characteristics: the one, feeding habits are assorted, can endanger 300 various plants that comprise forest, fruit tree, crops, flowers etc.; The 2nd, fertility is extremely strong, 1 year breeding three generations, and a female white moth annual raises up seed 3,000 ten thousand, can reach more than 200,000,000 at most; The 3rd, the route of transmission is wide, all can do long-distance communications with various goods, means of transport etc. throughout the year; The 4th, harm is serious, can eat up all green plants blades in the extremely short time during outburst.It is forestry pest, is pest again.In known technology, the method of existing control fall webworm mainly is to adopt chemical pesticide, though insect density can be fallen at short notice, seriously there are problems such as environmental pollution, use chemical pesticide that fall webworm are developed immunity to drugs in addition for a long time.Using the chemical pesticide control cotton bollworm is exactly precedent, because the abuse of chemical pesticide makes cotton bollworm produce very strong pesticide resistance, and the harm of main at present dependence plantation transgenic cotton against pests variety control cotton bollworm.Equally, if in a single day fall webworm produce similar pesticide resistance, will produce great disaster to ecotope; Be again utilize natural enemy such as all formulas nibble chalcid fly have still that breeding is slow, expense is high, to problems such as contemporary insect control effect are undesirable.
Summary of the invention
In order to overcome the deficiencies in the prior art, solve existing pesticide control fall webworm contaminated environment, the potential threat ecological safety is with the natural enemy control problem that breeding is slow again, expense is high.The present invention's purpose provides that a kind of preparation is easy, abundant raw material, easily large tracts of land is promoted, control efficiency is obvious, can directly prevent and treat fall webworm again can with mutual liquor and the making and use method thereof that delays the control fall webworm that fall webworm develop immunity to drugs that use of beta-cypermethrin.
The present invention addresses the above problem the technical scheme that is adopted:
A kind of Insect Resistant Cotton phosphate extract of preventing and treating fall webworm, it is by Insect Resistant Cotton blade, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and water, the Insect Resistant Cotton phosphate extract of the control fall webworm of making.
The Insect Resistant Cotton phosphate extract of above-mentioned control fall webworm, its preparation method comprises following technical step:
A, select the Insect Resistant Cotton raw material: selected Insect Resistant Cotton must be authorization above the provincial level, that contain bacillus thuringiensis,Bt (Bt) insecticidal proteins and be cotton plant the energy-conservation continuous generation Bt insecticidal proteins of whole growth season, lepidoptera pest such as cotton bollworm get the food Insect Resistant Cotton after its digestive system excellent kind of Insect Resistant Cotton being damaged and being poisoned to death;
The preparation of B, Insect Resistant Cotton cotton leaf: choose the intact leaf of above-mentioned Insect Resistant Cotton, the best is to push up downward the 3rd～4 layer blade from cotton plant, and it is standby to clean the back;
The preparation of C, phosphate buffer: with dipotassium hydrogen phosphate (K
2HPO
43H
2O) weighing adds the dipotassium hydrogen phosphate solution that water is prepared into 1mol/L; With potassium dihydrogen phosphate (KH
2PO
4) weighing, add the potassium dihydrogen phosphate that water is prepared into 1mol/L; By volumetric ratio be respectively: dipotassium hydrogen phosphate solution 187.2: potassium dihydrogen phosphate 12.8 is measured, with behind the two mixing in container the capacity of being settled to be 1000, the preparation become PH=7.9～8.1, concentration is the phosphate buffer of 0.2mol/L;
D, the preparation of Insect Resistant Cotton phosphate extract: take by weighing for the phosphate buffer 900～1200 of Insect Resistant Cotton blade 100: 0.2mol/L by weight ratio, the Insect Resistant Cotton blade is broken into the broken leaf of 10mm * 20mm～20mm * 40mm to be put into juice extractor and mixes with phosphate buffer and stir evenly, pour in the container then, in ultrasonic oscillator, shook 9～11 minutes, again after leaving standstill 20～26 hours under 1-5 ℃ the condition, filter with 50～100 order filters, promptly being mixed with concentration and being 1: 9～12 Insect Resistant Cotton phosphate extract (being called for short toxalbumin), is mother liquor with it.
The Insect Resistant Cotton phosphate extract of above-mentioned control fall webworm, its using method is: according to density situation and the algebraically of fall webworm, selection is that 1: 9～12 Insect Resistant Cotton phosphate extracts mother liquor to be diluted with water to concentration is that any Insect Resistant Cotton phosphate of 1: 15,1: 20,1: 25,1: 30,1: 40,1: 50 extracts soup with concentration, preferably is chosen in to spray in the larval phase before fall webworm become moth and prevents and treats.
Insect Resistant Cotton phosphate extract of control fall webworm of the present invention and making and use method thereof are at fall webworm growth life habit characteristics, in conjunction with mechanism and the effect of using for reference the cotton control of existing gene pest-resistant cotton bollworm, after through several laboratory active determination test, optimized preparation method by Insect Resistant Cotton blade, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and water, made with extra care out Insect Resistant Cotton phosphate extract, on probation obvious through overtesting and field to the fall webworm preventive effect.
When the present invention uses,, select for use the Insect Resistant Cotton phosphate of respective concentration to extract soup, preferably be chosen in the sprinkling of fall webworm low instar larvae phase and prevent and treat according to fall webworm insect pest situation.
Because the present invention designs and has adopted technique scheme, has solved existing pesticide control fall webworm contaminated environment effectively, the potential threat ecological safety, breeding is slow again with the natural enemy control, the problem that expense is high.Also through test and practical result show for several times; it is compared with existing and technology; has easy, the abundant raw material of preparation; easily large tracts of land is promoted; easy to use; control efficiency is obvious, can directly prevent and treat fall webworm and can prevent that fall webworm from developing immunity to drugs with mutual use of beta-cypermethrin again, is of value to advantage such as preserve the ecological environment.It is applicable to control fall webworm and other insect.
Description of drawings
Below in conjunction with accompanying drawing and example the present invention is described in further detail.
Fig. 1 is that fall webworm are got the death condition figure that food uses Insect Resistant Cotton water extract processing tree-of-heaven blade.
Fig. 2 is that fall webworm are got the death condition figure that food uses Insect Resistant Cotton phosphate buffer processing tree-of-heaven blade.
Fig. 3 is definite figure of Insect Resistant Cotton phosphate extract (toxalbumin) valid density.
Embodiment
Below in conjunction with example the present invention is described in further detail.
Embodiment 1:
Prepare the cotton leaf of raw material Insect Resistant Cotton, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and water.
Its preparation method step:
A, selection Insect Resistant Cotton raw material, select for use and authorize Insect Resistant Cotton kind Shandong cotton and grind 28, this kind contains bacillus thuringiensis,Bt (Bt) insecticidal protein gene and gets its digestive system of food back at lepidoptera pests such as the energy-conservation continuous generation Bt insecticidal proteins of whole growth season, cotton bollworms and is damaged and is poisoned to death;
The complete cotton leaf of above-mentioned excellent kind of Insect Resistant Cotton is chosen in the preparation of B, Insect Resistant Cotton cotton leaf, all is to push up downward the 3rd～4 layer cotton leaf from cotton plant, and it is standby to clean the back;
The preparation of C, phosphate buffer is with dipotassium hydrogen phosphate (K
2HPO
43H
2O) weighing is 22.6 kilograms, adds the dipotassium hydrogen phosphate solution that water is prepared into 1mol/L; With potassium dihydrogen phosphate (KH
2PO
4) 13.6 kilograms of weighings, add the potassium dihydrogen phosphate that water is prepared into 1mol/L; The case volumetric ratio is respectively: dipotassium hydrogen phosphate solution 187.2ml: potassium dihydrogen phosphate 12.8ml is measured, with behind the two mixing in container constant volume be 1000ml to capacity, the preparation become PH=8.0, concentration is the phosphate buffer of 0.2mol/L;
D, the preparation of Insect Resistant Cotton phosphate extract, with the Insect Resistant Cotton cotton leaf of above-mentioned preparation according to the proportioning weighing after, earlier be broken into 10 * 20～20 * 30 broken leaves with cracker, be by weight ratio: 00 kilogram of the phosphate buffer 1 of 10 kilograms in Insect Resistant Cotton blade: 0.2mol/L, stir with juice extractor and to squeeze the juice 4 minutes, become cotton leaf pastel, pour in the container, concussion is 10 minutes in ultrasonic oscillator, again after leaving standstill 24 hours under 3-4 ℃ the condition, filtering with 80 order filters, promptly be mixed with concentration and be 1: 10 Insect Resistant Cotton phosphate extract, is mother liquor with it.
Using method, the Insect Resistant Cotton phosphate extract of selecting for use was respectively concentration 1: 20,1: 25,1: 30 in urban district, Baoding in 2009, fall webworm spray in larval phase prevent and treat after, lethality was more than 95% in 9 days.
Fall webworm are got food and use Insect Resistant Cotton water extract to handle the death condition of tree-of-heaven blade
As shown in Figure 1, get food and use the larval mortality of Insect Resistant Cotton water extract processing back tree-of-heaven blade low, the average death number at the 9th day is 4.7, and lethality is 15.7%.The average dead number of clear water contrast is 2.3, and lethality is 7.7%.Be higher than the clear water contrast though water is put forward the lethality of Insect Resistant Cotton processing, compare with the lethality of getting food Insect Resistant Cotton blade, mutually far short of what is expected, it is not obvious to illustrate that water is carried the Insect Resistant Cotton treatment effect.
Fall webworm are got food and use Insect Resistant Cotton phosphate buffer (toxalbumin) to handle the death condition of tree-of-heaven blade
By accompanying drawing 2 as can be seen, 1: 10 processing the 7th day dead 30, dead 30 of 1: 15 and 1: 20 density control Ninth Heaven, three kinds of concentration lethality all can reach 100%; The processing of 1: 25,1: 30 concentration is respectively 24,20 at an average death number of the 9th day, and lethality is 80% and 66.7%.This shows that 5 kinds of concentration of treatment all have significantly killing effect to fall webworm, apparently higher than phosphate solution contrast and clear water contrast.
Determining of Insect Resistant Cotton phosphate extract (toxalbumin) valid density:
By accompanying drawing 3 as can be seen, handle at 1: 10 the 5th day dead 30, handle at 1: 30 the 6th day dead 30, dead 30 of 1: 50 density control seven days.
Claims (3)
1. an Insect Resistant Cotton phosphate extract of preventing and treating fall webworm is characterized in that it is by Insect Resistant Cotton blade, dipotassium hydrogen phosphate, potassium dihydrogen phosphate and water, the Insect Resistant Cotton phosphate extract of the control fall webworm of making.
2. according to the Insect Resistant Cotton phosphate extract of the described control of claim 1 fall webworm, it is characterized in that the preparation method of the Insect Resistant Cotton phosphate extract of these control fall webworm comprises following technical step:
A, select the Insect Resistant Cotton raw material: selected Insect Resistant Cotton must be authorization above the provincial level contain bacillus thuringiensis,Bt (Bt) insecticidal proteins and be cotton plant the energy-conservation continuous generation Bt insecticidal proteins of whole growth season, lepidoptera pest such as cotton bollworm get the food Insect Resistant Cotton after its digestive system excellent kind of Insect Resistant Cotton being damaged and being poisoned to death;
The preparation of B, Insect Resistant Cotton cotton leaf: choose the complete cotton leaf of above-mentioned excellent kind of Insect Resistant Cotton, the best is to push up downward the 3rd～4 layer cotton leaf from cotton plant, and it is standby to clean the back;
The preparation of C, phosphate buffer: with dipotassium hydrogen phosphate (K
2HPO
43H
2O) weighing adds the dipotassium hydrogen phosphate solution that water is prepared into 1mol/L; With potassium dihydrogen phosphate (KH
2PO
4) weighing, add the potassium dihydrogen phosphate that water is prepared into 1mol/L; By volumetric ratio be respectively: dipotassium hydrogen phosphate solution 187.2: potassium dihydrogen phosphate 12.8 is measured, with behind the two mixing in container constant volume be 1000 to capacity, the preparation become PH=7.9～8.1, concentration is the phosphate buffer of 0.2mol/L;
D, the preparation of Insect Resistant Cotton phosphate extract: take by weighing for the phosphate buffer 900～1200 of Insect Resistant Cotton blade 100: 0.2mol/L by weight ratio, the Insect Resistant Cotton blade is broken into the broken leaf of 10mm * 20mm～20mm * 40mm to be put into juice extractor and mixes with phosphate buffer and stir evenly, pour in the container then, in ultrasonic oscillator, shook 9～11 minutes, again after leaving standstill 20～26 hours under 1-5 ℃ the condition, filter with 50～100 order filters, promptly being mixed with concentration and being 1: 9～12 Insect Resistant Cotton phosphate extract, is mother liquor with it.
3. according to claim 1 or the 2 described Insect Resistant Cotton phosphate extracts of preventing and treating fall webworm, it is characterized in that, the using method of the Insect Resistant Cotton phosphate extract of these control fall webworm is: according to density situation and the algebraically of fall webworm, selection is that 1: 9～12 Insect Resistant Cotton phosphate extracts mother liquor to be diluted with water to concentration is that any Insect Resistant Cotton phosphate of 1: 15,1: 20,1: 25,1: 30,1: 40,1: 50 extracts soup with concentration, sprays in the larval phase before fall webworm become moth and prevents and treats.
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|---|---|---|---|
| CN2010102465753A CN101986843A (en) | 2010-08-04 | 2010-08-04 | Insect-resistant cotton phosphate extraction solution for controlling hyphantria cunea, production method and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668933A (en) * | 2012-05-31 | 2012-09-19 | 山东潍坊东盛园艺有限公司 | Method for cutting out hyphantria cunea net curtain |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
-
2010
- 2010-08-04 CN CN2010102465753A patent/CN101986843A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
Non-Patent Citations (2)
| Title |
|---|
| 吴志平等: "从转基因水稻中提取Bt毒蛋白的方法与效率", 《浙江大学学报(农业与生命科学版)》 * |
| 陈松等: "ELISA方法鉴定转Bt基因抗虫棉", 《江苏农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668933A (en) * | 2012-05-31 | 2012-09-19 | 山东潍坊东盛园艺有限公司 | Method for cutting out hyphantria cunea net curtain |
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Application publication date: 20110323 |