CN101985404A - Parent seed culture medium formula for high-temperature mushroom - Google Patents
Parent seed culture medium formula for high-temperature mushroom Download PDFInfo
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- CN101985404A CN101985404A CN2009101821438A CN200910182143A CN101985404A CN 101985404 A CN101985404 A CN 101985404A CN 2009101821438 A CN2009101821438 A CN 2009101821438A CN 200910182143 A CN200910182143 A CN 200910182143A CN 101985404 A CN101985404 A CN 101985404A
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- high temperature
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Abstract
The invention belongs to the technical field of edible mushroom, and particularly relates to a parent seed culture medium formula for high-temperature mushroom. The formula comprises 40 grams of corn meal, 20 grams of glucose, 20 grams of agar, 3 grams of peptone and 1,000 milliliters of water. The high-temperature mushroom hyphae cultured by the culture medium of the formula have highest growth speed, and the average growth speed is 14.61.
Description
Technical field: the invention belongs to the edible mushrooms technical field, be specifically related to a kind of prescription of mother culture media of high temperature flat mushroom.
Background technology: flat mushroom has another name called the north wind bacterium, in China's edible mushrooms gross output ratio, ranks first always.The flat mushroom fine and tender taste, delicious flavour. being of high nutritive value, is a kind of edible mushrooms that is loved by the people.At present, the area of summer culture high temperature flat mushroom constantly enlarges, Cultivar is on the increase, therefore, inquire into of the influence of different nutritional conditions to the growth of high temperature hypha of Pleurotus ostreatus, the substratum optimum formula of the suitable high temperature flat mushroom growth at different levels of screening is the needs that carry out high-yield culturing, is one of key measure that improves output and quality.
The flat mushroom kind is a lot, can be divided into high temperature modification, middle high temperature modification, wide warm higher type, wide medium-sized partially, the wide temperature of temperature type on the low side and middle low temperature modification by the temperature type, obtain the good quality and high output of flat mushroom, correctly selects kind very important.In raising variety, Yin Gaowen flat mushroom fruiting phase temperature height, disease is many, so the plantation amount is on the low side relatively.But the price of pliotherm period flat mushroom is 2～3 times of phase autumn and winter, and the mushroom farming of therefore planting the high temperature flat mushroom in recent years is more and more, but because of technical difficulty is big, success ratio is very low.Want to make high temperature flat mushroom high yield, stable yields, grasp the correct manufacture craft of bacterial classification, guarantee that the good quality of bacterial classification is very important.Facts have proved that the comparable inferior strain volume increase of breeding is more than 30%, and fruiting is strong, disease and pest is rare.Because the measured bacterial classification vitality of matter is strong, after planting mycelial growth is vigorous, thereby can suppress varied bacteria growing, simultaneously because mycelia is decomposed in substratum and to absorb the ability of nutrition strong, can carry nutrient and moisture for sporophore better, can obtain high yield, the stable yields of flat mushroom.
Summary of the invention: the objective of the invention is at above-mentioned present situation the mother culture media prescription of a kind of high temperature flat mushroom of proposition.
Technical scheme of the present invention is: Semen Maydis powder 40g, glucose 20g, agar 20g, peptone 3g, water 1000mL.
Embodiment: describe the present invention below in conjunction with embodiment and Comparative Examples.
Comparative Examples 1: potato 200g, glucose 20g, agar 20g, water 1000mL.
Comparative Examples 2: potato 20g, sucrose 20g, agar 20g, water 1000mL.
Comparative Examples 3: potato 200g, glucose 20g, agar 20g, peptone 5g, KH
2PO
42g,
MgSO
42g, V
B110mg, water 1000mL.
Comparative Examples 4: wheat bran 50g, glucose 20g, agar 20g, peptone 5g, KH
2PO
41g, MgSO
41g, water 1000mL.
Comparative Examples 5: the bright mushroom sheet of high temperature flat mushroom 250g, glucose 20g, agar 20s, peptone 3g, KH
2PO
42g, MgSO
42g, water 1000mL.
Embodiment: Semen Maydis powder 40g, glucose 20g, agar 20g, peptone 3g, water 1000mL.
After the substratum making method is made routinely with above-mentioned various prescriptions, be respectively charged in the triangular flask autoclaving 30min.Wherein bright mushroom is soaked the juice substratum and wants first bright mushroom sheet is put in people's water to boil 10min, after 4 layers of filtered through gauze, puts other composition of people again and makes required substratum (making of wheat bran synthetic medium is identical).The substratum of in Bechtop 6 prescriptions being handled is 25mL in the culture dish of the diameter of falling people 9cm respectively, and 3 sub-districts are established in every processing, 3 repetitions in every sub-district.Utilize punch tool with bacterial classification quantitatively be seeded in the prescription substratum central point, the inoculation point that punch tool is got is the circumference place of distance center bacterium piece 2cm.After the inoculation, place 25 ° of constant incubators to cultivate.
The embodiment of the invention prescription substratum high temperature hypha of Pleurotus ostreatus speed of growth is the fastest, and the average speed of growth is 14.61.
Claims (1)
1. the prescription of the mother culture media of a high temperature flat mushroom is characterized in that Semen Maydis powder 40g, glucose 20g, agar 20g, peptone 3g, water 1000mL.The mother culture media high temperature hypha of Pleurotus ostreatus speed of growth is the fastest, and the average speed of growth is 14.61.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101821438A CN101985404A (en) | 2009-07-28 | 2009-07-28 | Parent seed culture medium formula for high-temperature mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101821438A CN101985404A (en) | 2009-07-28 | 2009-07-28 | Parent seed culture medium formula for high-temperature mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101985404A true CN101985404A (en) | 2011-03-16 |
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ID=43709806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101821438A Pending CN101985404A (en) | 2009-07-28 | 2009-07-28 | Parent seed culture medium formula for high-temperature mushroom |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475136A (en) * | 2017-09-30 | 2017-12-15 | 山东省农业科学院农业资源与环境研究所 | A kind of special mother culture media of flat mushroom and preparation method and application |
-
2009
- 2009-07-28 CN CN2009101821438A patent/CN101985404A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475136A (en) * | 2017-09-30 | 2017-12-15 | 山东省农业科学院农业资源与环境研究所 | A kind of special mother culture media of flat mushroom and preparation method and application |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110316 |