(2) background technology
Fluazinam (Fluazinam) is the phenyl amines protective fungicide, and its mechanism of action is mitochondrial oxidation phosphorylated uncoupler, namely gives sprouting, mycelia breakthrough, growth and sporulation by the inhibition spore and suppresses infecting of pathogen.The fluazinam fungicidal spectrum is wide, and effect is better than the GPF (General Protection False bactericide.Applicable crops is wide in grape, apple, pears, oranges and tangerines, wheat, soybean, potato, tomato, cucumber, paddy rice, tea, lawn etc., and is very effective to interlink spore genus, Botrytis, Phytophthora, Plasmopara, Sclerotinia and Venturia bacterium.Botrytis cinerea to anti-benzimidazole type and dicarboximide class biocide agent also has good result.Resistance of rainwater washing against, the lasting period is long.Except bactericidal activity, fluazinam also has certain acaricidal activity, engraves mite, kamisawa tetranychus etc. as citrus red mite, China pink.
But fluazinam can't enter in plant corpus by plant surface without systemic activity and therapeutic action, although a lot of pathogens there is bactericidal activity preferably, really not ideal in the practical application of field.
Tebuconazole (Tebuconazole) is a kind of triazole bactericidal agent, and its mechanism of action is the sterol demethylation, namely passes through to suppress the biosynthesis of wheat sterol, kill pathogenic bacteria, thus prevent infecting of pathogen.Have treatment and protective effect preferably.Tebuconazole is widely used, and can be used for the control as wheat, barley, oat, rye, paddy rice, corn, peanut, soybean and the crop pests such as other oil crop, tomato, potato, Chinese sorghum, grape, asparagus, mango, pears, peach, apple, banana, tea tree and various vegetables thereof.
But because of factors such as Tebuconazole price and the good controls of tool, Tebuconazole single dose abuse phenomenon was more serious in recent years, had caused the generation of resistance, if can not effectively resolve this problem, will be subject to very big impact the service life of Tebuconazole.
(3) summary of the invention
One of purpose of the present invention is to overcome above-mentioned deficiency, but a kind of applied widely, effective, residual low delaying drug resistance is provided and increases the bactericidal composition that contains fluazinam and Tebuconazole in service life.
Two of purpose of the present invention is to provide a kind of purposes that contains the bactericidal composition of fluazinam and Tebuconazole.
The object of the present invention is achieved like this:
A kind of bactericidal composition that contains fluazinam and Tebuconazole, described composition contains fluazinam and Tebuconazole, and the weight ratio of described fluazinam and Tebuconazole is 1~60: 1~40.
The optimization weight ratio of described fluazinam and Tebuconazole is 4~30: 8~40.
The weight ratio of described fluazinam and Tebuconazole is 1: 1,1: 4,1: 8,1: 16,1: 20,4: 1,8: 1,12: 1 or 16: 1.
Described combination dosage form is any one formulation that allows on agricultural chemicals.
Described combination dosage form is wetting powder, water dispersible granules, suspending agent, aqueous emulsion or missible oil.
Described fluazinam and the Tebuconazole mass percent in composition is 1~90%.
A kind of purposes that contains the bactericidal composition of fluazinam and Tebuconazole is used for the medicine as cereal crop, melon and fruit or vegetable crop disease.
Be used for the medicine as pear scab.
Compared with prior art, the invention has the beneficial effects as follows:
(1) present composition synergistic effect of doing well in certain ratio range, the bactericidal effect of composition have had than single dose and have significantly improved, and have reduced simultaneously agricultural chemicals using dosage and cost, have reduced residually, have also alleviated the harmful effect to environment;
(2) in the present invention, the mechanism of action of two kinds of active components is different, and the application of composition can delay or overcome the resistance of pathogen, extends the service life of product;
(3) present composition scope of application increases, be applicable to prevent and treat the disease of the crops such as cereal crop, various melon and fruit, vegetables, crop pests such as paddy rice, wheat, tomato, banana, apple, pears particularly are as rice blast, wheat powdery mildew, early blight of tomato, alternaria leaf spot of apple, pear scab etc.
(4) embodiment
Clearer in order to be purpose of the present invention, technical scheme and advantage, the present invention describes with following instantiation, but the present invention is not limited to these examples.
The present composition finds through the indoor bioassay test take fluazinam and Tebuconazole as active ingredient, and after both are composite, antagonistic drug venereal disease evil has obvious synergistic function, specifically is illustrated with following biologicall test example.
The example of measuring in kind: the composite toxicity test to pear scab of fluazinam and Tebuconazole
1, subjects: pear scab
2, test method: with reference to " pesticide bioassay test rule NY/T1156.2-2006 ", adopt the pastille medium therapy.
3, data statistic analysis: analyze with the SAS6.12 statistical software.Calculate mycelial growth inhibition rate (%) according to test data, obtain virulence regression equation formula, correlation coefficient (r), EC
50And co-toxicity coefficient.
4, evaluation method: with reference to " pesticide bioassay test rule NY/T1156.2-2006 ", according to Sun﹠amp; The co-toxicity coefficient method (CTC) of Johnson (1960) is estimated the synergistic effect of pharmacy mix, and namely CTC≤80 are antagonism, and 80<CTC<120 are summation action, and CTC 〉=120 are synergistic effect.
The percentage composition of A medicament in the toxicity index of the theoretical toxicity index (TTI) of mixture=medicament A * 100 mixtures
The percentage composition of B medicament in the toxicity index of+medicament B * 100 mixtures
5, results and analysis
Fluazinam, Tebuconazole and both the combination that is mixed of different proportion the toxicity test of pear scab bacterium be the results are shown in Table 1, in table 1, " fluorine: penta " is writing a Chinese character in simplified form of " fluazinam: Tebuconazole ".
Table 1 fluazinam and Tebuconazole are mixed to the toxicity test result of pear cucumerinum
The indoor biometrics result shows: fluazinam and Tebuconazole are mixed with 1: 1,1: 4,1: 8,1: 16,1: 20,4: 1,8: 1,12: 1 and 16: 1 ratios pear cucumerinum is all shown synergistic effect, and be wherein better with the synergistic effect of 1: 1,1: 4 and 1: 16.Therefore, only from the synergy virulence relatively, can look 1: 1,1: 4 and 1: 16 is the best proportioning that fluazinam and Tebuconazole are mixed.As for which kind of ratio being mixed in production reality, still needing considers decision in conjunction with synergy virulence, Financial cost, processing technology and toxicity etc.
The present composition can claim to be fit to upper any one formulation used of agricultural with the preparation of known method, and wherein reasonable formulation has missible oil, water dispersible granules, aqueous emulsion, suspending agent and wetting powder, below describes with instantiation.
Embodiment 1:
Fluazinam 2.5g, Tebuconazole 2.5g, phenethyl phenol polyethenoxy ether what thing 3g of institute, this sulfoacid calcium of alkyl 1g, sorbitol polyoxyethylene ether 1.5g, polyvinyl alcohol-acetic acid polyethylene and ethylene copolymers 0.5g, reduction are supplied 100g than 0.5g, ethylene glycol 10g, organic silicone 0.2g, rhodamine toner 0.1g, water, mix, grind through sand mill, stir again, make the present composition 75% suspension seed-coating agent.
Embodiment 2:
With fluazinam 10g, Tebuconazole 40g, sodium methylene bis-naphthalene sulfonate 4.5g, sodium lignin sulfonate 3g, kaolin add, ethylene glycol 5g, xanthans 0.5g, kaolin supplies 100g, after mixing, through air-flow crushing, granulation, make the present composition 50% water dispersible granules again.
Embodiment 3:
With fluazinam 8g, Tebuconazole 12g, dodecyl sodium sulfate 5g, polyoxyethylene nonylphenol ether 3g, sodium methylene bis-naphthalene sulfonate 2g, xanthans 0.2g propane diols 5g, suitable quantity of water, to mix through wet grinding, homogenizing makes the present composition 20% suspending agent.
Embodiment 4:
Fluazinam 3g, Tebuconazole 48g, dodecyl sodium sulfate 4.5g, sodium lignin sulfonate 3g, white carbon 5g, kaolin are added to 100g, mix making the present composition 51% wetting powder through air-flow crushing.
Embodiment 5:
With fluazinam 2g, Tebuconazole 18g, dimethylbenzene 10g, alkylaryl formaldehyde resin APEO 10g, alkylphenol polyoxyethylene phosphate 1g, ethylene glycol 4g, xanthans 0.1g, organic silicone 0.1g, suitable quantity of water, the preparation oil phase, the preparation water, under high shear, with the even aqueous phase of oil phase or water is added in oil phase, shear and make the present composition 20% aqueous emulsion.
Application Example:
Utilize the preparation that above-described embodiment makes to prevent and treat the pear scab test of pesticide effectiveness.
1, test is processed: this test medicine consumption is established respectively three concentration for the treatment of according to the difference of each composition, and the contrast medicament is respectively commercially available agricultural chemicals 50% fluazinam suspending agent, 25% Tebuconazole wetting powder and clear water blank.
2, test method: test is chosen in pear scab over the years serious theatre to be carried out, and tests each age fruit tree of hanking and is no less than 2, repeats 4 times.Investigation method and evaluating drug effect are undertaken by GB/T17980.24-2000.
3, result of the test such as following table:
Annotate: a is illustrated on α=0.05 level significantly, and b is illustrated on α=0.05 level variant, c be illustrated in have on α=0.05 level not remarkable.
Field control effectiveness test shows, fluazinam and Tebuconazole bactericidal composition have good preventive effect to pear scab, and control efficiency is better than the single dose kind.