CN101983621A - Method for artificially collecting sperms of phasianidae animals - Google Patents
Method for artificially collecting sperms of phasianidae animals Download PDFInfo
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- CN101983621A CN101983621A CN 201010119037 CN201010119037A CN101983621A CN 101983621 A CN101983621 A CN 101983621A CN 201010119037 CN201010119037 CN 201010119037 CN 201010119037 A CN201010119037 A CN 201010119037A CN 101983621 A CN101983621 A CN 101983621A
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Abstract
The invention discloses a method for artificially collecting sperms of phasianidae animals. The method comprises the following steps: 1) massaging the waist, lower tall coverts, upper tail coverts and tail feathers of a phasianidae animal by a hand; and massaging two sides of a cloaca by the other hand at the same time until a degraded reproductive organ stretches out; and 2) extruding the two sides of the cloaca and collecting the excurrent sperms. The method of the invention is successfully used for carrying out artificial sperm collection on more than 20 types of endangered phasianidae animals, such as Syrmaticus reevesii, Temminck's Tragopan, Tragopan caboti, Tragopan satyra, silver pheasant, Crossoptilon mantchuricum, Crossoptilon auritum, white eared-pheasant, Crossoptilon crossoptilon, Lophophorus lhuysii, Lophophorus sclateri, Chrysolopus pictus, Chrysolophus amherstiae, peacock pheasant and the like; and the success rate reaches more than 90%.
Description
Technical field
The present invention relates to a kind of method of artificial semen collection, particularly a kind of method that is used for the artificial semen collection of Phasianidae animal.
Background technology
Phasianidae (Phasianidae) birds are monoids that have characteristic in the Chinese wildlife resource, China is the maximum country of rare in the world Phasiana class in imminent danger, have that kind is abundant, endemic species and the many characteristics of Endangered species, not only provide provenance for cultivating good poultry, and with the keeping of forest ecosystem health.Owing to reasons such as the forfeiture in habitat, irrational utilization, alien species invasion, climate changes, the problem of China's Phasiana class resource forfeiture is very outstanding.The rapid atrophy of the distribution of some peculiar Phasiana classes in imminent danger, population quantity significantly descends, and adds these Phasiana classes self anaematopoiesis, and survival prospects causes anxiety.Calendar year 2001, the National Development and Reform Committee and the State Administration of Forestry approve to execute " national wild animals and plants protection and natural reserve construction engineering overall planning (2001-2050) ".According to planning, China's emphasis has been implemented the rescue of 15 class rare and endangered species such as giant panda, Zhu Ibis, tiger, crane class, Phasiana class, the orchid family and cycadophyte and has been bred engineering, comprises the protection wild stocks and actively develops ex situ conservation.
The open-air typical habitat natural environment of birds in imminent danger is various, and the place is open, and the suitable condition that is suitable for its courtship display, selection, pursuit spouse, copulation etc. is arranged.Under artificial breeding's condition; be subjected to the influence of factors such as space, behavior; can not finish said process; ovum natural fertilization rate under the condition of raising in cages is low; this is a ubiquitous in the world difficult problem; seriously restrict the large-scale development of caged animal population, be difficult to reach the purpose of the Phasiana class rare in imminent danger of protection.In the last few years, artificial insemination deeply more and more came into one's own, and as under the support of United States Department of Agriculture (USDA), wild animal and Fishery Bureau, the technology of artificial insemination of crane class in imminent danger is set up.But as China lay special stress on protecting the important birds monoid of another one---the Phasianidae AVIAN ARTIFICIAL INSEMINATION does not make a breakthrough as yet.Artificial insemination comprises artificial semen collection, semen analysis and artificial insemination's technology, and wherein emphasis and difficult point are artificial semen collections, and artificial semen collection is one of important technical links of rare Phasiana class artificial breeding in imminent danger and ex situ conservation.
Adopt existing poultry semen collection method to collect seminal fluid from wild fowl, semen collection success rate first not high (generally being lower than 20%), second too big to the injury of wild Phasiana class, adopt 1 essence in this way after, the Phasiana class is crouched down in nest motionlessly in the quite a long time, and performance is terrified unusual.Domestic also have a seminal fluid of gathering Phasiana class in imminent danger by electrostimulation, has 2 problems utilizing poultry semen collection method collecting semen to be run into equally, and after electricity irritation, fecaluria and seminal fluid together flow out, and cause seminal fluid to use.
Summary of the invention
The object of the present invention is to provide a kind of method that is used for the artificial semen collection of Phasianidae animal, and be specially adapted to the method for the artificial semen collection of wild Phasianidae animal.
The method of the artificial semen collection of Phasianidae animal provided by the invention may further comprise the steps:
1) with hands massage Phasianidae animal waist, lower tall coverts, upper tail coverts and a tail feathers; Simultaneously with another hands massage cloaca both sides; Stretch out until the genitals who degenerates;
2) push the cloaca both sides then, collect effusive seminal fluid.
Available nontransparent head-shield is lived the headgear of Phasianidae animal before described step 1).Above-mentioned head-shield specifically can be the head-shield that cotton is made.
Massage from top to bottom above-mentioned massage cloaca both sides.
Massage speed in the step of above-mentioned massage cloaca both sides is 1-1.5 time/second, preferably 1.5 times/second.
Further, the size of the power of the massage in the step of above-mentioned massage cloaca both sides is 1-2.5 newton, preferably 1.5 newton.
Above-mentioned massage waist, lower tall coverts, upper tail coverts and tail feathers comprise the steps:
Other that will be except that thumb four refer to and together, relative with thumb and parallel, the afterbody of Phasianidae animal is placed between thumb and other and the finger together, and make finger orientation vertical with the tail feathers direction, the centre of the palm is downward, thumb is close to afterbody and is caudad moved along lower tall coverts, and other fingers and palm while caudad divide a word with a hyphen at the end of a line along waist, the upper tail coverts of bird, the palm upset of taking advantage of a situation in the process that massage is divided a word with a hyphen at the end of a line.
Described speed of massaging the massage in waist, lower tall coverts, upper tail coverts and the tail feathers step simultaneously is 1-1.5 time/second, is preferably 1.5 times/second.
The angle of above-mentioned upset is the 110-150 degree, is preferably 120 degree.
Power in above-mentioned massage waist, lower tall coverts, upper tail coverts and the tail feathers step is 2-4 newton, is preferably 2 newton.
The firmly size of described extruding is 5-10 newton, preferably 6 newton.
Above-mentioned Phasianidae animal is brown eared pheasant, blue eared pheasant, Baima chicken, tangun chicken, Syrmaticus reevesii (Gray), Tragopan temminckii, yellow ventral horn Phasiana, satyr tragopan, Lophura nycthemera, green tail monal, white smear monal, golden pheasant with red belly, Chrvsolophus amherstiae (Leadubeater) or peacock pheasant slightly.
The present invention successfully be brown eared pheasant, blue eared pheasant, Baima chicken, tangun chicken, Syrmaticus reevesii (Gray), Tragopan temminckii, yellow ventral horn Phasiana, satyr tragopan, Lophura nycthemera, green tail monal, white smear slightly the Phasiana class in imminent danger of kind more than 20 successful implementations such as monal, golden pheasant with red belly, Chrvsolophus amherstiae (Leadubeater), peacock pheasant artificial semen collection, success rate is more than 90%.Simultaneously method of the present invention can be Phasiana class in imminent danger and implements artificial insemination smart source is provided, and also can bring into play those and can't finish the value of the outstanding male of copulation because of wound, remedies the defective that excellent individual can not be inseminated by artificial collecting semen.
The method of the artificial semen collection of Phasianidae animal of the present invention can be next step screening of carrying out artificial insemination, fine quality seminal fluid and lays a good foundation; for the ex situ conservation of rare Phasiana class in imminent danger provides smart source, for the rapid expansion of the artificial population of Phasiana class in imminent danger with take off danger and establish important foundation.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment, but the present invention is not limited to following examples.
Among the following embodiment, if no special instructions, be conventional method.
Embodiment 1, artificial semen collection
The tangun chicken is carried out artificial semen collection, and the concrete steps of artificial semen collection are as follows:
1) make the eyeshield of a 10cm * 10cm with deceiving cotton, three faces are stitched into pocket-like, and one side is encircled the limit threading, behind the threading, pulls at slightly and can close up; The black cotton tape for preparing is enclosed within on the tangun Caput Gallus domesticus, so both can have avoided its fear and the defecation or the pollution seminal fluid of urinating, can not make it frightened and do not arrange seminal fluid again;
2) then can adopt a people to control another person's semen collection; Also birds can be placed on the artificial dummy of the shape of a saddle, a people independently finishes semen collection.What present embodiment adopted is that the Phasiana class is placed on the artificial dummy of the shape of a saddle, and a people independently finishes semen collection.
Massage waist, lower tall coverts, upper tail coverts and the tail feathers of Phasiana class earlier with left hand.The concrete steps of massage be that except that thumb other of left hand are pointed also together, relative with thumb and parallel, afterbody is placed between thumb and other fingers, and make finger orientation vertical with the tail feathers direction, the centre of the palm is downward, thumb is close to afterbody and is caudad moved along lower tall coverts, other fingers and palm while are along the waist of bird, upper tail coverts is caudad divided a word with a hyphen at the end of a line, the thumb and the forefinger that lay respectively at afterbody back of the body side and abdomen side all move to apart from tail base portion 15 centimeters, palm is taken advantage of a situation simultaneously to overturn about 120 ° and is got back to initial position continuation massage next time then in the process that massage is divided a word with a hyphen at the end of a line, in the process of whole massage, it is motionless that left forearm and wrist position keep.The speed of massage is 1.5 times/s.The power of massage is 2 newton.
Simultaneously massage tangun chicken cloaca both sides from top to bottom with hand thumb and forefinger simultaneously, speed is 1.5 times/s, and firmly size is 1.5 newton, and the genitals who degenerates until the tangun chicken stretches out.
3) right-handed immediately thumb and forefinger extruding tangun chicken cloaca both sides, the firmly size of described extruding is 6 newton.Genitals's (papillary copulatory organ that the Phasiana class is residual) that the tangun chicken degenerates can expose, and flows out white seminal fluid from intermediary groove, then collects seminal fluid with container.If the smart cup of large-scale Phasiana class (as green tail monal) available set is collected seminal fluid, other wild Phasiana class (body weight is less than 2000g) seminal fluid generally are less than 0.15ml, available semen collection tubule (volume 0.3ml, long 13.3cm, Valley Vet Supply company, Kansas USA) collects seminal fluid.Present embodiment adopts the semen collection tubule to come the seminal fluid of semen collection tangun chicken.Detect semen collection to the seminal fluid volume be 0.05-0.15ml.
Claims (10)
1. the method for the artificial semen collection of Phasianidae animal may further comprise the steps:
1) with hands massage Phasianidae animal waist, lower tall coverts, upper tail coverts and a tail feathers; With another hands massage cloaca both sides, stretch out simultaneously until the genitals who degenerates;
2) push the cloaca both sides then, collect effusive seminal fluid.
2. method according to claim 1 is characterized in that: with nontransparent head-shield the headgear of Phasianidae animal was lived before described step 1); Massage from top to bottom described massage cloaca both sides.
3. method according to claim 1 and 2 is characterized in that: the massage speed in the step of described massage cloaca both sides is 1-1.5 time/second, preferably 1.5 times/second.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: the size of the power of the massage in the step of described massage cloaca both sides is 1-2.5 newton, preferably 1.5 newton.
5. according to arbitrary described method among the claim 1-4, it is characterized in that: described massage waist, lower tall coverts, upper tail coverts and tail feathers comprise the steps:
Other that will be except that thumb four refer to and together, relative with thumb and parallel, the afterbody of Phasianidae animal is placed between thumb and other and the finger together, and make finger orientation vertical with the tail feathers direction, the centre of the palm is downward, thumb is close to afterbody and is caudad moved along lower tall coverts, and other fingers and palm while caudad divide a word with a hyphen at the end of a line along waist, the upper tail coverts of bird, the palm upset of taking advantage of a situation in the process that massage is divided a word with a hyphen at the end of a line.
6. method according to claim 5 is characterized in that: described speed of massaging the massage in waist, lower tall coverts, upper tail coverts and the tail feathers step simultaneously is 1-1.5 time/second, is preferably 1.5 times/second.
7. according to claim 5 or 6 described methods, it is characterized in that: the angle of described upset is the 110-150 degree; Be preferably 120 degree.
8. according to arbitrary described method among the claim 5-7, it is characterized in that: the power in described massage waist, lower tall coverts, upper tail coverts and the tail feathers step is 2-4 newton, preferably 2 newton.
9. according to arbitrary described method among the claim 1-8, it is characterized in that: the firmly size of described extruding is 5-10 newton, preferably 6 newton.
10. according to arbitrary described method among the claim 1-9, it is characterized in that: described Phasianidae animal is brown eared pheasant, blue eared pheasant, Baima chicken, tangun chicken, Syrmaticus reevesii (Gray), Tragopan temminckii, yellow ventral horn Phasiana, satyr tragopan, Lophura nycthemera, green tail monal, white smear monal, golden pheasant with red belly, Chrvsolophus amherstiae (Leadubeater) or peacock pheasant slightly.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010119037 CN101983621A (en) | 2010-03-05 | 2010-03-05 | Method for artificially collecting sperms of phasianidae animals |
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| CN 201010119037 CN101983621A (en) | 2010-03-05 | 2010-03-05 | Method for artificially collecting sperms of phasianidae animals |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369907A (en) * | 2011-09-17 | 2012-03-14 | 兰州宏庆科技有限公司 | Method for breeding special wild chicken through artificial fertilization |
| CN110538000A (en) * | 2019-10-16 | 2019-12-06 | 西华师范大学 | sperm storage cup and sperm collection device for wild pheasant artificial sperm collection device |
| CN111387136A (en) * | 2020-03-24 | 2020-07-10 | 四川蜂桶寨国家级自然保护区管理局 | Hatching method of green tail rainbow pheasant |
| CN118147319A (en) * | 2024-04-01 | 2024-06-07 | 广州动物园 | Genetic marker for silver pheasant individual identification and application |
-
2010
- 2010-03-05 CN CN 201010119037 patent/CN101983621A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《中国鸟类学研究--第四届海峡两岸鸟类学术研讨会文集》 20001231 张雁云 鸟类的人工受精 说明书第328页第I部分第3段 1-10 , 2 * |
| 《动物学杂志》 19981231 潘庆杰等 野生动物人工采精方法探讨 55-58 1-10 第33卷, 第5期 2 * |
| 《首届中国黄羽肉鸡行业发展大会会刊》 20081231 林敏 人工授精在黄羽肉鸡育种和生产中的应用 93-96 1-10 , 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102369907A (en) * | 2011-09-17 | 2012-03-14 | 兰州宏庆科技有限公司 | Method for breeding special wild chicken through artificial fertilization |
| CN110538000A (en) * | 2019-10-16 | 2019-12-06 | 西华师范大学 | sperm storage cup and sperm collection device for wild pheasant artificial sperm collection device |
| CN111387136A (en) * | 2020-03-24 | 2020-07-10 | 四川蜂桶寨国家级自然保护区管理局 | Hatching method of green tail rainbow pheasant |
| CN111387136B (en) * | 2020-03-24 | 2022-10-14 | 四川蜂桶寨国家级自然保护区管理局 | Hatching method of green tail rainbow pheasant |
| CN118147319A (en) * | 2024-04-01 | 2024-06-07 | 广州动物园 | Genetic marker for silver pheasant individual identification and application |
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Application publication date: 20110309 |