CN101971029B - 电化学检测金属标记的分析物 - Google Patents

电化学检测金属标记的分析物 Download PDF

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Publication number
CN101971029B
CN101971029B CN200880125056.2A CN200880125056A CN101971029B CN 101971029 B CN101971029 B CN 101971029B CN 200880125056 A CN200880125056 A CN 200880125056A CN 101971029 B CN101971029 B CN 101971029B
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China
Prior art keywords
method described
analyte
metal
solid support
releasing agent
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Expired - Fee Related
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CN200880125056.2A
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English (en)
Chinese (zh)
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CN101971029A (zh
Inventor
罗伯特·安德鲁·波特
马泰乌兹·希曼斯基
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agplus Diagnostics Ltd
Secco Holdings Ltd
UK Secretary of State for Business Innovation and Skills
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UK Secretary of State for Innovation Universities and Skills
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Filing date
Publication date
Priority claimed from GB0723137A external-priority patent/GB0723137D0/en
Priority claimed from GB0812679A external-priority patent/GB0812679D0/en
Priority claimed from GB0812845A external-priority patent/GB2462062A/en
Application filed by UK Secretary of State for Innovation Universities and Skills filed Critical UK Secretary of State for Innovation Universities and Skills
Publication of CN101971029A publication Critical patent/CN101971029A/zh
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Publication of CN101971029B publication Critical patent/CN101971029B/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Secondary Cells (AREA)
CN200880125056.2A 2007-11-26 2008-11-25 电化学检测金属标记的分析物 Expired - Fee Related CN101971029B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB0723137A GB0723137D0 (en) 2007-11-26 2007-11-26 Electrochemical assay
GB0723137.6 2007-11-26
GB0812679.9 2008-07-10
GB0812679A GB0812679D0 (en) 2008-07-10 2008-07-10 Sample carrier for effecting chemical assays
GB0812845A GB2462062A (en) 2008-07-14 2008-07-14 Electrochemical assay
GB0812845.6 2008-07-14
PCT/GB2008/003931 WO2009068862A1 (en) 2007-11-26 2008-11-25 Electrochemical detection using silver nanoparticle labelled antibodies

Publications (2)

Publication Number Publication Date
CN101971029A CN101971029A (zh) 2011-02-09
CN101971029B true CN101971029B (zh) 2013-08-07

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CN200880125056.2A Expired - Fee Related CN101971029B (zh) 2007-11-26 2008-11-25 电化学检测金属标记的分析物

Country Status (9)

Country Link
US (1) US8337692B2 (enExample)
EP (1) EP2220494B1 (enExample)
JP (1) JP5055433B2 (enExample)
CN (1) CN101971029B (enExample)
AT (1) ATE533054T1 (enExample)
AU (1) AU2008328588B2 (enExample)
GB (1) GB2458420B (enExample)
NZ (1) NZ585851A (enExample)
WO (1) WO2009068862A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2462062A (en) * 2008-07-14 2010-01-27 Sec Dep For Innovation Univers Electrochemical assay
JP5806892B2 (ja) 2010-09-30 2015-11-10 シスメックス株式会社 検出物質の電気化学的検出方法および被検物質の電気化学的検出方法
JP5762225B2 (ja) * 2011-09-08 2015-08-12 新留　康郎 銀シェル金ナノロッドを用いる分析方法
GB201118293D0 (en) 2011-10-24 2011-12-07 Sec Dep For Business Innovation & Skills The Electrochemical assay
GB201217390D0 (en) 2012-09-28 2012-11-14 Agplus Diagnostics Ltd Test device and sample carrier
RU2550955C1 (ru) * 2013-12-11 2015-05-20 Общество с ограниченной ответственностью "Уральский центр биофармацевтических технологий Способ электрохимического иммуноанализа для определения вирусов/антигенов вирусов
CN104181292B (zh) * 2014-08-27 2015-12-02 湖南师范大学 一种蛋白质单分子水平安培免疫分析方法
JP6486065B2 (ja) * 2014-10-28 2019-03-20 シスメックス株式会社 被検物質の検出方法
US10598625B2 (en) 2015-04-08 2020-03-24 Board of Regents, The University System of Texas Methods and systems for the detection of analytes
WO2016167724A1 (en) * 2015-04-13 2016-10-20 Agency For Science, Technology And Research Device for electrochemical immunoassay and method of fabricating the same
WO2018094113A1 (en) 2016-11-16 2018-05-24 University Of Washington Systems and methods for cyclic fluorescence imaging
WO2020000959A1 (zh) * 2018-06-29 2020-01-02 北京华科泰生物技术股份有限公司 一种基于标记物标记的电化学检测方法
JP7310697B2 (ja) * 2019-05-16 2023-07-19 Tdk株式会社 被検物質の分析キット、及び被検物質の分析方法
EP3851855A1 (en) * 2020-01-17 2021-07-21 Technische Universität München Sensing of molecules by electrochemical detection of nanoparticles

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030186274A1 (en) * 2000-06-26 2003-10-02 Benoit Limoges Electrochemical immunoassays using colloidal metal markers
WO2005121792A1 (en) * 2004-06-07 2005-12-22 Inverness Medical Switzerland Gmbh Method

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096669A (en) 1988-09-15 1992-03-17 I-Stat Corporation Disposable sensing device for real time fluid analysis
GB9318677D0 (en) * 1993-09-09 1993-10-27 Kodak Ltd Method and apparatus for measuring silver ion activity
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20010055812A1 (en) 1995-12-05 2001-12-27 Alec Mian Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
US6709869B2 (en) 1995-12-18 2004-03-23 Tecan Trading Ag Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
WO1998028623A1 (en) 1996-12-20 1998-07-02 Gamera Bioscience Corporation An affinity binding-based system for detecting particulates in a fluid
US6096272A (en) 1997-05-23 2000-08-01 Becton Dickinson & Company Automated microbiological testing apparatus and methods therefor
US6300138B1 (en) 1997-08-01 2001-10-09 Qualigen, Inc. Methods for conducting tests
CA2320296A1 (en) 1998-05-18 1999-11-25 University Of Washington Liquid analysis cartridge
IL126776A (en) * 1998-10-27 2001-04-30 Technion Res & Dev Foundation A method of investing gold
AU5646800A (en) 1999-03-02 2000-09-21 Helix Biopharma Corporation Card-based biosensor device
IL151477A0 (en) 2000-03-09 2003-04-10 Clinical Analysis Corp Medical diagnostic system
US8518328B2 (en) 2005-12-27 2013-08-27 Honeywell International Inc. Fluid sensing and control in a fluidic analyzer
US20040248093A1 (en) 2000-11-27 2004-12-09 Coombs James Howard Magneto-optical bio-discs and systems including related methods
AU2003262742A1 (en) 2002-08-19 2004-03-03 Iowa State University Research Foundation, Inc. Redox polymer nanoparticles
US7838296B2 (en) 2002-08-28 2010-11-23 Separation Technology, Inc. Methods and apparatus for ultrasonic determination of red blood cell indices
US20040058457A1 (en) 2002-08-29 2004-03-25 Xueying Huang Functionalized nanoparticles
US20040259076A1 (en) 2003-06-23 2004-12-23 Accella Scientific, Inc. Nano and micro-technology virus detection method and device
US7208784B2 (en) 2003-10-07 2007-04-24 Quantum Logic Devices, Inc. Single-electron transistor for detecting biomolecules
US8158062B2 (en) 2003-11-05 2012-04-17 Chris Dykes Disposable fluid sample collection device
US8343074B2 (en) 2004-06-30 2013-01-01 Lifescan Scotland Limited Fluid handling devices
EP1825269A4 (en) 2004-12-13 2012-06-06 Univ South Carolina SURFACE-REINFORCED RAMAN SPECTROSCOPY USING SHAPED GOLDNANOPARTICLE
WO2006118420A1 (en) 2005-04-30 2006-11-09 Jae Chern Yoo Bio-disc, bio-driver apparatus, and assay method using the same
US20060263818A1 (en) 2005-05-23 2006-11-23 Axel Scherer High throughput multi-antigen microfluidic fluorescence immunoassays
US20060270049A1 (en) 2005-05-25 2006-11-30 Board Of Supervisors Of Louisiana State University & A & M College Enhanced detection of analytes on surfaces using gold nanoparticles
EP2041573B1 (en) 2006-06-23 2019-09-04 PerkinElmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
KR101153748B1 (ko) 2008-05-07 2012-06-14 재단법인서울대학교산학협력재단 바이오센서로 유용한 새로운 형태의 금／은 코어쉘 복합체
US8372435B2 (en) 2008-06-23 2013-02-12 Princeton University Modular monolayer coatings for selective attachment of nanoparticles to biomolecules

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030186274A1 (en) * 2000-06-26 2003-10-02 Benoit Limoges Electrochemical immunoassays using colloidal metal markers
WO2005121792A1 (en) * 2004-06-07 2005-12-22 Inverness Medical Switzerland Gmbh Method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
An Electrochemical Metalloimmunoassay Based on a Colloidal Gold Label;Murielle Dequaire，et al;《Anal. Chem.》;20001031;第72卷(第22期);全文 *
Synthesis and electrochemical applications of gold nanoparticles;Shaojun Guo，et al;《Analytica Chimica Acta,》;20070829;第598卷(第2期);全文 *

Also Published As

Publication number Publication date
JP2011504593A (ja) 2011-02-10
CN101971029A (zh) 2011-02-09
ATE533054T1 (de) 2011-11-15
WO2009068862A8 (en) 2009-10-22
GB2458420B (en) 2009-10-28
AU2008328588B2 (en) 2013-09-05
US20100320092A1 (en) 2010-12-23
EP2220494A1 (en) 2010-08-25
GB2458420A8 (en) 2009-10-07
AU2008328588A1 (en) 2009-06-04
EP2220494B1 (en) 2011-11-09
US8337692B2 (en) 2012-12-25
GB0912670D0 (en) 2009-08-26
GB2458420A (en) 2009-09-23
WO2009068862A1 (en) 2009-06-04
JP5055433B2 (ja) 2012-10-24
NZ585851A (en) 2011-04-29

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Effective date of registration: 20151127

Address after: England Hampshire

Patentee after: AGPLUS DIAGNOSTICS LTD.

Address before: England Hampshire

Patentee before: SECCO Holdings Ltd.

Effective date of registration: 20151127

Address after: England Hampshire

Patentee after: SECCO Holdings Ltd.

Address before: London, England

Patentee before: THE SECRETARY OF STATE FOR BUSINESS,INNOVATION & SKILLS OF HER MAJESTY'S BRITANNIC GOVERNMENT

Effective date of registration: 20151127

Address after: London, England

Patentee after: THE SECRETARY OF STATE FOR BUSINESS,INNOVATION & SKILLS OF HER MAJESTY'S BRITANNIC GOVERNMENT

Address before: London, England

Patentee before: The Secretary of State for Innovation Universities & Skills of Her Majesty's Britannic Government

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Granted publication date: 20130807