CN101965399B - 用于生产乙醇酸的固定的微生物腈水解酶的改进 - Google Patents

用于生产乙醇酸的固定的微生物腈水解酶的改进 Download PDF

Info

Publication number
CN101965399B
CN101965399B CN200880124121.XA CN200880124121A CN101965399B CN 101965399 B CN101965399 B CN 101965399B CN 200880124121 A CN200880124121 A CN 200880124121A CN 101965399 B CN101965399 B CN 101965399B
Authority
CN
China
Prior art keywords
glutaraldehyde
nitrilase
enzyme
catalyst
enzyme catalyst
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200880124121.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN101965399A (zh
Inventor
R·迪科西莫
A·本-巴萨特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
High Purity Technology Science Co ltd
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CN101965399A publication Critical patent/CN101965399A/zh
Application granted granted Critical
Publication of CN101965399B publication Critical patent/CN101965399B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/584—Recycling of catalysts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN200880124121.XA 2007-10-31 2008-10-31 用于生产乙醇酸的固定的微生物腈水解酶的改进 Expired - Fee Related CN101965399B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/930550 2007-10-31
US11/930,550 US7695945B2 (en) 2007-10-31 2007-10-31 Immobilized microbial nitrilase for production of glycolic acid
PCT/US2008/081948 WO2009059101A1 (en) 2007-10-31 2008-10-31 Improvement in immobilized microbial nitrilase for production of glycolic acid

Publications (2)

Publication Number Publication Date
CN101965399A CN101965399A (zh) 2011-02-02
CN101965399B true CN101965399B (zh) 2016-06-08

Family

ID=40243876

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200880124121.XA Expired - Fee Related CN101965399B (zh) 2007-10-31 2008-10-31 用于生产乙醇酸的固定的微生物腈水解酶的改进

Country Status (8)

Country Link
US (1) US7695945B2 (https=)
EP (1) EP2215225B1 (https=)
JP (1) JP5702604B2 (https=)
CN (1) CN101965399B (https=)
DK (1) DK2215225T3 (https=)
ES (1) ES2563482T3 (https=)
PL (1) PL2215225T3 (https=)
WO (1) WO2009059101A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2627666B1 (de) 2010-10-12 2015-07-08 c-LEcta GmbH Nitrilasen mit gesteigerte aktivität
GB2554708A (en) * 2016-10-05 2018-04-11 Chemoxy Int Process for converting nitriles
CN111848495B (zh) * 2020-06-02 2022-06-21 山东华素制药有限公司 一种1-苄基-3-哌啶醇的合成方法
CN115845880B (zh) * 2022-12-08 2024-06-14 复旦大学 一种水溶性高分子模板原位构筑过渡金属硫化物/碳复合材料及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1409756A (zh) * 1999-11-12 2003-04-09 三菱丽阳株式会社 灭活的微生物细胞

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2175805A (en) 1937-06-08 1939-10-10 Du Pont Stabilized organic nitrile and process of making
US2890238A (en) 1957-01-31 1959-06-09 Dow Chemical Co Preparation of glyconitrile
GB1553662A (en) 1978-04-13 1979-09-26 Union Carbide Corp Sterilization of fermentation media
DK146942C (da) 1978-04-19 1984-07-30 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt
US4355105A (en) 1981-03-30 1982-10-19 Miles Laboratories, Inc. Glutaraldehyde/polyethylenimine immobilization of whole microbial cells
US5187301A (en) 1989-10-26 1993-02-16 W. R. Grace & Co.-Conn. Preparation of iminodiacetonitrile from glycolonitrile
EP0486289B1 (en) 1990-11-14 1997-07-09 Nitto Chemical Industry Co., Ltd. Biological process for producing alpha-hydroxyamide or alpha-hydroxy acid
JP3354688B2 (ja) 1994-01-28 2002-12-09 三菱レイヨン株式会社 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
US6037155A (en) 1997-02-27 2000-03-14 Nippon Soda Co., Ltd. Process for preparing α-hydroxy acids using microorganism and novel microorganism
US6551804B2 (en) 1999-07-12 2003-04-22 E. I. Du Pont De Nemours And Company Process for preparing 4-cyanopentanoic acid
US6251646B1 (en) * 1999-07-12 2001-06-26 E. I. Du Pont De Nemours And Company Method for stabilizing nitrilase activity and preserving microbial cells
AU4972601A (en) 2000-03-31 2001-10-15 Du Pont Isolation and expression of a gene for a nitrilase from acidovorax facilis 72w
US6416980B1 (en) 2001-02-23 2002-07-09 E. I. Du Pont De Nemours & Company Method for producing glycolic acid from glycolonitrile using nitrilase
KR100869623B1 (ko) 2004-04-27 2008-11-21 미쓰이 가가쿠 가부시키가이샤 하이드록시 카복실산류의 생산 방법
US7148051B2 (en) 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
WO2006069114A2 (en) 2004-12-22 2006-06-29 E.I. Dupont De Nemours And Company Process for producing glycolic acid from formaldehyde and hydrogen cyanide
US7198927B2 (en) 2004-12-22 2007-04-03 E. I. Du Pont De Nemours And Company Enzymatic production of glycolic acid
US7368492B2 (en) 2004-12-22 2008-05-06 E. I. Du Pont De Nemours And Company Process for the synthesis of glycolonitrile
US7445917B2 (en) 2004-12-22 2008-11-04 E.I. Du Pont De Nemours And Company Process for producing glycolic acid from formaldehyde and hydrogen cyanide
WO2007036235A1 (en) * 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1409756A (zh) * 1999-11-12 2003-04-09 三菱丽阳株式会社 灭活的微生物细胞

Also Published As

Publication number Publication date
US7695945B2 (en) 2010-04-13
JP2011501969A (ja) 2011-01-20
PL2215225T3 (pl) 2016-05-31
CN101965399A (zh) 2011-02-02
EP2215225B1 (en) 2015-12-02
WO2009059101A1 (en) 2009-05-07
DK2215225T3 (en) 2016-03-07
ES2563482T3 (es) 2016-03-15
JP5702604B2 (ja) 2015-04-15
US20090111158A1 (en) 2009-04-30
EP2215225A1 (en) 2010-08-11

Similar Documents

Publication Publication Date Title
CN102016018B (zh) 用于生产乙醇酸的固定的微生物腈水解酶的改进
EP2217714B1 (en) Sequestration of formaldehyde to stabilize nitrilase specific activity when converting glycolonitrile to glycolic acid
US8110382B2 (en) Process for producing glycolic acid from formaldehyde and hydrogen cyanide
CN101133161B (zh) 由甲醛和氰化氢生产乙醇酸的方法
CN101965399B (zh) 用于生产乙醇酸的固定的微生物腈水解酶的改进

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20180108

Address after: Delaware, USA

Patentee after: THE CHEMOURS COMPANY FC, LLC

Address before: Delaware, USA

Patentee before: E. I. du Pont de Nemours and Co.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20231008

Address after: West Virginia, USA

Patentee after: High Purity Technology Science Co.,Ltd.

Address before: Delaware

Patentee before: THE CHEMOURS COMPANY FC, LLC

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160608

CF01 Termination of patent right due to non-payment of annual fee