CN101955897A - Functional strain No.3 as well as culture method and application thereof - Google Patents
Functional strain No.3 as well as culture method and application thereof Download PDFInfo
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- CN101955897A CN101955897A CN 201010267196 CN201010267196A CN101955897A CN 101955897 A CN101955897 A CN 101955897A CN 201010267196 CN201010267196 CN 201010267196 CN 201010267196 A CN201010267196 A CN 201010267196A CN 101955897 A CN101955897 A CN 101955897A
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Abstract
The invention discloses a functional strain No.3 as well as a culture method and application thereof. The functional strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCCNo.3936 and a classification Latin name of bacillus subtilis. The functional strain can be used for promoting plantlet growth, enhancing the output and the quality of tea, improving fruit quality, resisting pathogenic bacteria, decomposing slightly soluble inorganic phosphorus and organophosphorus into soluble phosphorus, building up fertility, obviously improving soil quality and the like. The functional strain can also grow in a 7% NaCl culture medium.
Description
Technical field
The present invention relates to a kind of function stem, relate in particular to a kind of function stem No. three and the application of cultural method and microbial inoculum.
Background technology
Because life-time service chemical fertilizer and agricultural chemicals cause problems such as soil compaction, land deterioration, chemical fertilizer and pesticide residue easily, bio-feritlizer is developed rapidly, is used to promote seedling growth, anti-microbial pathogen, improves edatope, aspect such as build up fertility.
In the prior art, there are problems such as function singleness, quality instability, soil suitability difference in bio-feritlizer.
Summary of the invention
The purpose of this invention is to provide the good function stem No. three of a kind of complete function, steady quality, soil suitability and the application of cultural method and microbial inoculum thereof.
The objective of the invention is to be achieved through the following technical solutions:
Function stem of the present invention No. three, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
The cultural method that above-mentioned function stem of the present invention is No. three is characterized in that this function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, this microbial inoculum comprise above-mentioned function stem No. three, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention:
This microbial inoculum is used to promote seedling growth;
This microbial inoculum is used for anti-microbial pathogen;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, improves the sugar degree of following one or more fruit: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
As seen from the above technical solution provided by the invention, function stem of the present invention No. three, can be used to promote seedling growth, anti-microbial pathogen, with insoluble inorganic phosphorus and organophosphorus be decomposed into titanium pigment, improve fruit quality, build up fertility etc.Complete function, steady quality, soil suitability are good.
Preservation information
Conserving microorganism (strain) classification name: subtilis;
Conserving microorganism (strain) Latin formal name used at school: Bacillus subtilis;
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC);
Depositary institution address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica;
Preservation date: on June 22nd, 2010;
Deposit number: CGMCC No.3936.
Embodiment
Function stem of the present invention No. three, its preferable embodiment is:
This function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
This function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, its preferable embodiment be, this microbial inoculum comprises above-mentioned function stem No. three, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention comprises following one or more application:
This microbial inoculum is used to promote seedling growth, such as being used for promoting following one or more seedling growths: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves; This microbial inoculum can also be used for improving the content that tealeaves has the tea-polyphenol of anti-cancer function;
This microbial inoculum is used for anti-microbial pathogen, is used to prevent and treat the generation of continuous cropping watermelon blight such as this microbial inoculum;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, such as the sugar degree that is used to improve following one or more fruit: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
The screening process of function stem of the present invention:
The applicant is to China fir, willow, Pinus massoniana Lamb, in the soil quality evolution research process of artificial forest such as eucalyptus, to being positioned at different terrain, under the different soils land occupation condition, main reproducting tree species under the different stand types, finished the investigation of 1000 polylith temporary sample plots and permanent sample plot, meanwhile, under different land occupation conditions, secondary forest under the different stand types, primary forest has also been done a large amount of sample ground investigation, in the research process, soil microflora to above-mentioned every research sample ground, done corresponding probe research, the nocardia kind of having found these research sample ground distributes, the dominant population regularity of distribution particularly, and present closely related with the soil fertility and the increase in standing timber.
On the basis of above-mentioned research, do function yeast screening: to the dominant genera kind separate, purifying and biological characteristic research, and expand numerous, germination test, seedbed test, find that these bacterial strains all have the function of obvious promotion seedling growth.
Further do disease-resistant test, find that these bacterial strains have anti-microbial pathogen function in various degree, the applicant can effectively prevent and treat the generation of continuous cropping watermelon blight in Daxing Pang Guo village evidence.
Do the test of anti-inorganic salt and show, find that these bacterial strains can grow in 7% NaCl substratum.Do the insoluble phosphorus decomposition run, find that these bacterial strains have the insoluble of decomposition inorganic phosphorus, organophosphorus is the function of titanium pigment.
The applicant produces solid and the liquid bacterial agent of developing these bacterial strains through a large amount of for many years indoor and outdoor tests.
Application effect:
On main reproducting tree species such as eucalyptus, Pinus massoniana Lamb and willow, use, obvious effect of increasing production, improve the increase in standing timber, generally more than 10%, use seedling stage, increase effect and reach more than the 20%-30%, have tangible growth promoting function, seedling stage to use the growth effect to ornamental tree species as happy eastern lily magnolia, sweet osmanthus etc. and reach more than the 20%-30%.Be applied to economic forest seeds such as tealeaves, increasing degree reaches 30-40%, and moreover, improving effectively has the anti-cancer function polyphenol content in the tealeaves.Improve the quality of fruit, improve the sugar degree of apple, oranges and tangerines, pears and peach.
The biomaterial feature that function stem of the present invention is No. three:
Referring to annex: the check probation report that Institute of Microorganism, Academia Sinica provides, the detection probation report about No. 3 bacterial strains wherein are the biomaterial feature of function stem of the present invention No. three.
The above; only for the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (10)
1. a function stem is No. three, it is characterized in that, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
2. the cultural method of the described function stem of claim 1 No. three is characterized in that this function stem grows in 7% NaCl substratum.
3. a microbial inoculum is characterized in that, this microbial inoculum comprises the described function stem of claim 1 No. three, and this microbial inoculum is solid or liquid bacterial agent.
4. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used to promote seedling growth.
5. the application of microbial inoculum according to claim 4 is characterized in that, described nursery stock comprise following one or more: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves;
Described microbial inoculum also is used for improving the content that tealeaves has the tea-polyphenol of anti-cancer function.
6. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used for anti-microbial pathogen.
7. the application of microbial inoculum according to claim 6 is characterized in that, this microbial inoculum is used to prevent and treat the generation of continuous cropping watermelon blight.
8. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment.
9. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used to improve the quality of fruit, improves the sugar degree of following one or more fruit: apple, oranges and tangerines, pears, peach.
10. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum and chemical fertilizer mix and executes, and are used to build up fertility.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010267196 CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010267196 CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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| CN101955897A true CN101955897A (en) | 2011-01-26 |
| CN101955897B CN101955897B (en) | 2012-08-22 |
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| CN 201010267196 Active CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250786A (en) * | 2011-05-21 | 2011-11-23 | 福建农林大学 | Inorganic phosphorus dissolving bacterium capable of improving chlorophyll content of eucalyptus |
| CN102399713A (en) * | 2011-09-22 | 2012-04-04 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
-
2010
- 2010-08-27 CN CN 201010267196 patent/CN101955897B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| 《应用与环境生物学报》 20031225 陶光灿 等 芽胞杆菌属(Bacillus sp.)10 株细菌混合制剂对4 种作物出苗及苗期生长的影响 598-602 1-10 第9卷, 第6期 2 * |
| 《林业科学研究》 19961230 王守宗 等 细菌肥料对杨树生长效应的研究 654-657 1-10 第9卷, 第6期 2 * |
| 《生态环境》 20031231 王光华 等 解磷菌的研究现状与展望 96-101 1-10 第12卷, 第1期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250786A (en) * | 2011-05-21 | 2011-11-23 | 福建农林大学 | Inorganic phosphorus dissolving bacterium capable of improving chlorophyll content of eucalyptus |
| CN102399713A (en) * | 2011-09-22 | 2012-04-04 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
| CN102399713B (en) * | 2011-09-22 | 2013-01-30 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
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| Publication number | Publication date |
|---|---|
| CN101955897B (en) | 2012-08-22 |
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Owner name: BEIJING ABT BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: FORESTRY INSTITUE, CHINESE INST.OF FORESTRY SCIENCES Effective date: 20140610 |
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Effective date of registration: 20140610 Address after: 102200 Changping District science and Technology Park, Beijing Road No. 11 Patentee after: BEIJING ABT BIOTECHNOLOGY CO., LTD. Address before: 100091, No. 2 East Hospital, Beijing, Haidian District Patentee before: Research Institute of Forestry, Chinese Academy of Forestry |