CN101955897B - Functional strain No.3 as well as culture method and application thereof - Google Patents
Functional strain No.3 as well as culture method and application thereof Download PDFInfo
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- CN101955897B CN101955897B CN 201010267196 CN201010267196A CN101955897B CN 101955897 B CN101955897 B CN 101955897B CN 201010267196 CN201010267196 CN 201010267196 CN 201010267196 A CN201010267196 A CN 201010267196A CN 101955897 B CN101955897 B CN 101955897B
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Abstract
The invention discloses a functional strain No.3 as well as a culture method and application thereof. The functional strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCCNo.3936 and a classification Latin name of bacillus subtilis. The functional strain can be used for promoting plantlet growth, enhancing the output and the quality of tea, improving fruit quality, resisting pathogenic bacteria, decomposing slightly soluble inorganic phosphorus and organophosphorus into soluble phosphorus, building up fertility, obviously improving soil quality and the like. The functional strain can also grow in a 7% NaCl culture medium.
Description
Technical field
The present invention relates to a kind of function stem, relate in particular to a kind of function stem No. three and the application of cultural method and microbial inoculum.
Background technology
Because life-time service chemical fertilizer and agricultural chemicals cause problems such as soil compaction, land deterioration, chemical fertilizer and pesticide residue easily, bio-feritlizer is developed rapidly, is used to promote seedling growth, anti-microbial pathogen, improves edatope, aspect such as build up fertility.
In the prior art, there are problems such as function singleness, quality instability, soil suitability difference in bio-feritlizer.
Summary of the invention
The purpose of this invention is to provide the good function stem No. three of a kind of complete function, steady quality, soil suitability and the application of cultural method and microbial inoculum thereof.
The objective of the invention is to realize through following technical scheme:
Function stem of the present invention No. three, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
The cultural method that above-mentioned function stem of the present invention is No. three is characterized in that this function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, this microbial inoculum comprise above-mentioned function stem No. three, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention:
This microbial inoculum is used to promote seedling growth;
This microbial inoculum is used for anti-microbial pathogen;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, the sugar degree of one or more fruit below improving: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
Technical scheme by the invention described above provides can be found out, function stem of the present invention No. three, can be used to promote seedling growth, anti-microbial pathogen, with insoluble inorganic phosphorus and organophosphorus be decomposed into titanium pigment, improve fruit quality, build up fertility etc.Complete function, steady quality, soil suitability are good.
Preservation information
Conserving microorganism (strain) classification name: subtilis;
Conserving microorganism (strain) Latin formal name used at school: Bacillus subtilis;
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC);
Depositary institution address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica;
Preservation date: on June 22nd, 2010;
Deposit number: CGMCC No.3936.
Embodiment
Function stem of the present invention No. three, its preferable embodiment is:
This function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
This function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, its preferable embodiment be, this microbial inoculum comprises above-mentioned function stem No. three, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention comprises following one or more application:
This microbial inoculum is used to promote seedling growth, such as being used for promoting following one or more seedling growths: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves; This microbial inoculum can also be used for improving the content that tealeaves has the tea-polyphenol of anti-cancer function;
This microbial inoculum is used for anti-microbial pathogen, is used to prevent and treat the generation of continuous cropping watermelon blight such as this microbial inoculum;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, such as the sugar degree that is used to improve following one or more fruit: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
The screening process of function stem of the present invention:
The applicant is in the soil quality evolution research process to artificial forest such as China fir, willow, Pinus massoniana Lamb, eucalyptus; To being positioned at the main reproducting tree species under different terrain, the different soils land occupation condition, under the different stand type; Accomplished the investigation of 1000 polylith temporary sample plots and permanent sample plot; Meanwhile, the secondary forest under the different land occupation conditions, under the different stand type, primary forest have also been done a large amount of appearance ground investigation, in the research process; Soil microflora to above-mentioned every research appearance ground; Done corresponding probe research, found nocardia kind distribution, particularly the dominant population regularity of distribution on these research appearance ground, and appeared closely related with the soil fertility and the increase in standing timber.
On the basis of above-mentioned research, do function yeast screening: to the dominant genera kind separate, purifying and biological characteristic research, and expand numerous, germination test, seedbed test, find that these bacterial strains all have the function of obvious promotion seedling growth.
Further do disease-resistant test, find that these bacterial strains have anti-microbial pathogen function in various degree, applicant's Pang Guo village evidence in the Daxing can effectively prevent and treat the generation of continuous cropping watermelon blight.
Do the test of anti-inorganic salt and show, find that these bacterial strains can grow in 7% NaCl substratum.Do the insoluble phosphorus decomposition run, find that these bacterial strains have the insoluble of decomposition inorganic phosphorus, organophosphorus is the function of titanium pigment.
The applicant produces solid and the liquid bacterial agent of developing these bacterial strains through a large amount of for many years indoor and outdoor tests.
Application effect:
On main reproducting tree species such as eucalyptus, Pinus massoniana Lamb and willow, use; Obvious effect of increasing production improves the increase in standing timber, generally more than 10%; Use seedling stage; Increase effect and reach more than the 20%-30%, have tangible growth promoting function, seedling stage to use the growth effect to ornamental tree species like happy eastern lily magnolia, sweet osmanthus etc. and reach more than the 20%-30%.Be applied to economic forest seeds such as tealeaves, increasing degree reaches 30-40%, and moreover, improving effectively has the anti-cancer function polyphenol content in the tealeaves.Improve the quality of fruit, improve the sugar degree of apple, oranges and tangerines, pears and peach.
The biomaterial characteristic that function stem of the present invention is No. three:
Referring to annex: the check probation report that Institute of Microorganism, Academia Sinica provides, the detection probation report about No. 3 bacterial strains wherein are the biomaterial characteristic of function stem of the present invention No. three.
The above; Be merely the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technician who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (4)
1. a function stem is No. three, it is characterized in that, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3936;
Classification called after: subtilis;
The Latin formal name used at school is: Bacillus subtilis.
2. the cultural method of the described function stem of claim 1 No. three is characterized in that this function stem grows in 7% NaCl substratum.
3. a microbial inoculum that is used to promote seedling growth is characterized in that, this microbial inoculum comprises the described function stem of claim 1 No. three, and this microbial inoculum is solid or liquid bacterial agent.
4. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used to promote seedling growth, said nursery stock comprise following one or more: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010267196 CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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| CN 201010267196 CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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| CN101955897A CN101955897A (en) | 2011-01-26 |
| CN101955897B true CN101955897B (en) | 2012-08-22 |
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| CN 201010267196 Active CN101955897B (en) | 2010-08-27 | 2010-08-27 | Functional strain No.3 as well as culture method and application thereof |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250786B (en) * | 2011-05-21 | 2013-03-20 | 福建农林大学 | Inorganic phosphorus dissolving bacterium capable of improving chlorophyll content of eucalyptus |
| CN102399713B (en) * | 2011-09-22 | 2013-01-30 | 华南农业大学 | Bacillus subtilis HL-1 and application thereof in respect of soil phosphate dissolving |
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Owner name: BEIJING ABT BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: FORESTRY INSTITUE, CHINESE INST.OF FORESTRY SCIENCES Effective date: 20140610 |
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Free format text: CORRECT: ADDRESS; FROM: 100091 HAIDIAN, BEIJING TO: 102200 CHANGPING, BEIJING |
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Effective date of registration: 20140610 Address after: 102200 Changping District science and Technology Park, Beijing Road No. 11 Patentee after: BEIJING ABT BIOTECHNOLOGY CO., LTD. Address before: 100091, No. 2 East Hospital, Beijing, Haidian District Patentee before: Research Institute of Forestry, Chinese Academy of Forestry |