CN104561179A - Microbial secondary metabolite palmatine hydrochloride and application thereof - Google Patents
Microbial secondary metabolite palmatine hydrochloride and application thereof Download PDFInfo
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Abstract
The invention relates to a microbial secondary metabolite palmatine hydrochloride and application thereof and belongs to the technical field of biological pesticides. The secondary metabolite is prepared by the following steps: (a), producing a strain of L.antibioticus YFY02 with the collection number of CGMCC NO.2403; (b) culturing the strain YFY02 for two days by utilizing a KB culture medium, performing enlarged culture by adopting a conventional fermentation method, extracting the fermentation liquor by using ethyl acetate, and performing rotary evaporation, thereby obtaining a crude metabolite extract; (c) by utilizing liquid chromatography, Sephadex LH-20 gel column chromatography, mass spectrometry, nuclear magnetic resonance and other spectroscopic techniques, and separating, purifying and identifying the crude extract of the secondary metabolite; and (d) identifying to obtain the effective secondary metabolite palmatine hydrochloride. The invention also discloses application of the microbial secondary metabolite palmatine hydrochloride in the preparation of agents for preventing and controlling bacterial blight of rice, bacterial leaf streak and plasmodiophora brassicae woronin. The microbial secondary metabolite disclosed by the invention has the advantages of environment friendliness, low cost and excellent effects of preventing and controlling the rice bacterial diseases and plasmodiophora brassicae woronin.
Description
Technical field:
The present invention relates to a kind of microbial secondary metabolite palmatin hydrochloride and application thereof, microorganism belonging to genus pesticide production technology field.
Background technology:
Bacterial leaf streak of rice, infected by the raw pvs oryzae and oryzicola (Xanthomonoas oryzae pv.oryzicola) of rice Xanthomonas campestris rice and cause, be distributed widely in the subtropical and tropical zones in Asia, domesticly mainly be distributed in south China, Central China, southwestern Deng Dao district, now become one of the Major Diseases in south China rice district.When this disease occurs, spread rapidly, cause rice leaf turn yellow withered, unfilled gtains increases, production declining, can reach 40-60% time serious, what this disease was serious threatens Rice Production.
Bacterial blight of rice, infected by rice Xanthomonas campestris paddy rice pvs oryzae and oryzicola (Xanthomonas oryzae pv.oryzae) and cause, be distributed widely in global each paddy rice producing region, be domesticly mainly distributed in south China each province, northern rice field is also serious all the more in recent years; Except Xinjiang and Northern-Northeast China, this disease is the important disease affecting China's rice safety production always.Bacterial blight of rice, in the history having more than 70 years of China, is the second largest disease being only second to rice blast.When this disease occurs, spread rapidly, blade is withered, spreads to the production loss in grave illness field up to 60%.
Crop in cruciferae is various, the Cruciferae club root caused is infected by rape plasmodiophora brassicae (Plasmodiophora brassicae woron), annual onset area reaches more than 2,000 ten thousand mu (secondary), have a strong impact on brassicaceous vegetable quality and yield, underproduction 70-80% is caused time serious, and germ can, in soil more than Survival for 10 Years, prevent and treat very difficult.
For disease on above-mentioned staple crops and vegetables, due to all kinds of chemical pesticide of long-term a large amount of use, drug-fast strain constantly occurs, strengthens usage amount and the frequency of utilization of chemical pesticide; Chemical pesticide uses and remains the export trade seriously hindering economic crops simultaneously.Along with our people's growth in the living standard, the safe mass problem of the agricultural product such as vegetables receives shows great attention to, especially Pesticide Residue.Residue of pesticide can cause chemical pesticide to remain for a long time in natural world and food chain, cause the serious consequences such as environmental pollution, ecological balance destruction, social effects of pollution.Conservative Disease management measure as: agricultural cultivation measure, disease-resistant variety etc., effect is all undesirable.Microorganism formulation have little to non-target organism safety, toxic and side effect, to features such as environment compatibility are good, gain great popularity in control plant pest.Especially in today that mankind's environmental consciousness strengthens, adopt efficient, nontoxic, safe noresidue, microbial pesticide easy to use substitutes the common recognition that the large chemical pesticide of current side effect progressively becomes countries in the world.Therefore, researching and developing the biologic product of efficient, nontoxic, safe noresidue, control of plant disease easy to use, has been problem extremely urgent in China's agricultural development.
Bacterial strain YFY 02 is separated the Rhizosphere of Crops from bamboo plantation town, Fuyuan County, Yunnan Province, the qualification results such as combining form, cultural colony, customary physiological biochemistry and the full-automatic identification systems of Biolog, are accredited as the molten bacillus of antibiotic (Lysobacter antibioticus).This research department to the control efficiency of the diseases such as crucifer club root, bacterial blight of rice and bacterial leaf streak of rice, has carried out separation, purifying, qualification and biological and ecological methods to prevent plant disease, pests, and erosion application to the secondary metabolite of this bacterial strain based on antibiotic molten bacillus strain YFY 02 zymotic fluid.By literature search, the open report utilizing antibiotic molten bacillus secondary metabolite palmatin hydrochloride to prevent and treat paddy rice bacteriosis and crucifer club root is had no.
Summary of the invention:
The object of the invention is to the deficiency overcoming prior art, a kind of microbial secondary metabolite palmatin hydrochloride and application thereof of efficient, nontoxic, safe noresidue are provided.
The molten bacillus YFY 02 of production bacterial strain antibiotic of the present invention preservation.Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center; Address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date: on 03 17th, 2008; Preserving number: CGMCC NO.2403.
The molten bacillus YFY 02 of production bacterial strain antibiotic of the present invention, bacterial strain YFY 02 is separated the Rhizosphere of Crops from bamboo plantation town, Fuyuan County, Yunnan Province.This bacterial strain has following characteristics: (1) bacterium colony is circular, opaque thin liquid shape, and initial-stage culture is dark yellow, and the later stage transfers sepia or pitchy bacterium colony to, thalline elongated rod shape; (2) this bacterial strain antimicrobial spectrum is wide, surely can grow for a long time, have stronger antagonism to the pathogenetic bacteria such as Xanthomonas oryzae, Population of Xanthomonas Oryzae Pv, have obvious inhibitory action to pathogen plasmodiophora spore germination at brassicaceous vegetable rhizosphere and rice leaf.
Microbial secondary metabolite palmatin hydrochloride of the present invention obtains through following steps:
A. producing bacterial strain is the molten bacillus of antibiotic (Lysobacter antibioticus) YFY02, preserving number: CGMCC NO.2403;
B. utilize KB medium culture bacterial strain YFY02 two days later, adopt common fermentation processes to carry out expansion and cultivate, fermentation liquor is obtained cometabolism crude extract by carrying out rotary evaporation after extraction into ethyl acetate;
C. utilize the spectroscopic technique of conventional liquid chromatography, Sephadex LH-20 gel filtration chromatography, infrared spectrum (IR), ultraviolet spectra (UV), optically-active (polarimeter), mass spectrum (MS), nuclear magnetic resonnance (NMR), dichroscope spectrum (CD), cometabolism crude extract is carried out being separated, purifying and identifying; Liquid-phase chromatographic analysis condition is: chromatographic column, Diamonsil C
18post (4.6mm × 200mm, 5 μm); Mobile phase, CH
3cN:H
2o (4:6); Determined wavelength 235nm; Column temperature 40 DEG C, flow velocity 1mL/min, sample size 20 μ L;
D. effective secondary metabolite qualification result of antibiotic molten bacillus strain YFY02 is: 2,3,9,10-tetramethoxy-5,13a-dihydro-6H-isoquino, i.e. palmatin hydrochloride (Palmatine chloride), molecular formula is: C21H20NO4, and structural formula is as follows:
Microbial secondary metabolite palmatin hydrochloride of the present invention has following feature: (1) has stronger pH adaptive capacity, is suitable in the soil of different pH values, and biocontrol effect is stablized; (2) to temperature, there is stronger adaptive capacity, be adapted to Different Altitude, different regions, Various Seasonal; (3) have stronger tolerance to ultraviolet, this ability is conducive to the stronger high altitude localities of ultraviolet.
Microbial secondary metabolite palmatin hydrochloride of the present invention, Comprehensive Traits is good, is easy to suitability for industrialized production; Good inhibitory action is had to Xanthomonas oryzae, Population of Xanthomonas Oryzae Pv bacterium, Cruciferae pathogen plasmodiophora, can as the application preparing water prevention rice bacterial leaf spot pathogenic bacteria, Population of Xanthomonas Oryzae Pv bacterium, Cruciferae pathogen plasmodiophora preparation.
The present invention, compared with existing agricultural chemicals, has that environmentally safe, cost are low, an advantage of control paddy rice bacteriosis and Cruciferae club root excellent.
Embodiment:
The step that the present embodiment microbial secondary metabolite palmatin hydrochloride used describes by summary of the invention part obtains.
The main controlling object of palmatin hydrochloride of the present invention is paddy bacterial disease and Cruciferae club root; Palmatin hydrochloride of the present invention has carried out efficiency test in the field on the ground such as Kunming, Yunnan suburbs, Dehong prefecture Ruili City, demonstrates this secondary metabolites preventive effect remarkable, stable, effectively can control paddy bacterial disease and Cruciferae club root.
Palmatin hydrochloride of the present invention is biological generally to be used in crop sowing or the disease phase of starting.Method is before sowing, adopts palmatin hydrochloride and seed to soak after (mixing) plant, dries in being manured into soil, or in the disease phase of starting, this preparation is passed through spray application.
Palmatin hydrochloride of the present invention can with other kill bacterial pesticide, plant growth regulator is used in combination, but must ensure mixed medicament the palmatin hydrochloride that the present invention adopts is had no side effect.
Bacterial strain YFY02 Comprehensive Traits is better, and secondary metabolites palmatin hydrochloride output is higher, is easy to suitability for industrialized production.
Be described in further detail the present invention by embodiment below, but content of the present invention is not limited thereto.
Embodiment one: the stability test test of palmatin hydrochloride
1. for examination material:
Strains tested: xanthomonas oryzae pv. oryzicola strong toxic strain YM15, Xanthomonas oryzae strong toxic strain FUJ, antibiotic molten bacillus strain YFY 02; For test preparation: palmatin hydrochloride; For examination medium: KB medium, NA medium; Reagent agent: sodium hydroxide, hydrochloric acid; For test instrument: pH instrument, water-bath, uviol lamp, aseptic working platform, ultraviolet/visible light spectrophotometer.
2. test method
2.1 palmatin hydrochlorides are to the mensuration of acid-base value, temperature and ultraviolet tolerance
The acquisition of palmatin hydrochloride prepares detailed process with reference to biologic product, and palmatin hydrochloride solution is 1ug/mL for examination concentration, and the addition in the cup of Oxford is 100uL/ cup; Carry disease germs dull and stereotyped for adding 50uL 3 × 10 in 20mL NA medium
8the Population of Xanthomonas Oryzae Pv YM15 of cfu/mL or leaf spot bacteria FUJ suspension, for subsequent use after shaking up condensation.Contrast with sterile water, the addition of sterile water in the cup of Oxford is 100uL/ cup.After 28 DEG C of cultivation 2-3d, observe and measure inhibition zone size.
PH Stability Determination: regulate palmatin hydrochloride solution ph with HC1 and NaOH, make its pH value be respectively 2,4,6,8,10, room temperature places 24h, after filtration sterilization, carry out dull and stereotyped bacteriostatic test, test the impact of different acid-base condition on palmatin hydrochloride bacteriostatic activity.With the palmatin hydrochloride solution of not adjust ph in contrast (pH is for 6.3).
Temperature stability measures: palmatin hydrochloride solution is placed in respectively 30 DEG C, 40 DEG C, 50 DEG C, 70 DEG C, 95 DEG C water-baths and processes 1h, after filtration sterilization, carry out dull and stereotyped bacteriostatic test, and test treatment of different temperature is on the impact of palmatin hydrochloride bacteriostatic activity.The palmatin hydrochloride solution of 1h is placed as blank (25 DEG C) using room temperature.
Ultraviolet tolerance measures: palmatin hydrochloride solution is placed in uviol lamp (40w, wavelength 254nm) under, distance 30cm, processes 1h, 3h, 6h, 12h, 24h respectively, after filtration sterilization, test the impact of different ultraviolet irradiation time on palmatin hydrochloride bacteriostatic activity.Using the palmatin hydrochloride solution of non-irradiated with ultraviolet radiation as blank.
2.2 palmatin hydrochlorides are to the measurement result of acid-base value, temperature and ultraviolet tolerance
Result of the test shows, palmatin hydrochloride has stronger soda acid adaptability, warm tolerance, ultraviolet tolerance.The field trial in different pH values, different temperatures, Different Altitude (ultraviolet) area can be widely used in, be conducive to carrying out and the Application and Development of the product such as biopesticide of antibiotic molten bacillus metabolite-palmatin hydrochloride field Biological Control Test.
The toxicity test of palmatin hydrochloride under the different acid-base condition of table 1
The toxicity test of palmatin hydrochloride under table 2 condition of different temperatures
The toxicity test of palmatin hydrochloride under the different time of ultraviolet irradiation of table 3
Embodiment two: palmatin hydrochloride adjusts the sick test of field control of bacterial stripe to paddy rice
1. for examination material
Strains tested: xanthomonas oryzae pv. oryzicola strong toxic strain YM15, antibiotic molten bacillus strain YFY 02; For test preparation: palmatin hydrochloride, 1% Shen piperazine mycin suspending agent, 1000 times of liquid, the peaceful 100 times of liquid of tikka; For examination medium: KB medium, NA medium; For studying thing: when landlord transplants rice seedlings kind moral excellent 13, red excellent No. 4 of cecospora spot disease-resistant variety; Controlling object: bacterial leaf streak of rice;
Experiment place: rice test field, Dehong prefecture Ruili City, Yunnan Province, middle fertility, plantation landlord transplant rice seedlings kind-De excellent 13, and community is single file district, area 30m
2, each process establishes 3 repetitions, randomized arrangement, totally 9 communities, and surrounding establishes protection row, and red excellent No. 4 of protection behavior cecospora spot disease-resistant variety, by the standardized management of high-grade rice cultivation step.
2. test method
2.1 bacterial leaf streak of rice controlling experiment
The preparation of xanthomonas oryzae pv. oryzicola YM15 bacteria suspension: after cecospora spot bacterial strain YM15 is activated 48h, be transplanted to the flat lining out of NA and cultivate 48h (28 DEG C), pathogen is made bacteria suspension with under aseptic washing from solid culture medium, shaking table shakes up, with spectrophotometer, bacteria suspension cell concentration is equivalent to 3 × 10
8cfu/mL, for inoculation.
Process one: antibiotic molten bacillus YFY02 secondary metabolites palmatin hydrochloride preparation spray inoculation (booting stage) on rice leaf by concentration being first 1ug/mL, inoculum concentration is 100mL/ strain, next day Inoculated Rice Xanthomonas campestris PV.oryzicola YM15 bacteria suspension, inoculum concentration is 100mL/ strain; Process two: only connect xanthomonas oryzae pv. oryzicola YM15 bacteria suspension, inoculum concentration is 100mL/ strain; Contrast: using sterile water spray inoculation paddy rice as blank, inoculum concentration is 100mL/ strain; Each process arranges 3 repetitions, inoculates 21 days " Invest, Then Investigate "s, investigates the disease number of sheets, and sick leaf-size class value, total number of sheets, calculate the incidence of disease, disease index.
Investigation record and severity Scaling standard as follows:
0 grade: without scab.
1 grade: lesion area accounts for less than 10% of leaf area.
3 grades: lesion area accounts for 11% ~ 25% of leaf area.
5 grades: lesion area accounts for 26% ~ 45% of leaf area.
7 grades: lesion area accounts for 46% ~ 65% of leaf area.
9 grades: lesion area accounts for more than 65% of leaf area.
The total number of sheets × 100% of the incidence of disease=investigation morbidity number of sheets/investigation
Disease index=[Σ (diseased plant at different levels (leaf) number × this sick level value)/investigate total strain (leaf) number × highest sick value of series] × 100%
Relative control effect=(contrast disease index-process disease index) × 100/ contrast disease index
2.2 bacterial leaf streak of rice control effect testing results
Palmatin hydrochloride shows the measurement result of rice streak field efficacy: palmatin hydrochloride can be stablized, inhibit the generation of bacterial leaf streak of rice efficiently, the incidence of disease, disease index comparatively control group all significantly reduce, preventive effect, up to 60.1%, shows the application and development prospect (table 4) that it is potential.
Table 4 palmatin hydrochloride measures rice streak field efficacy
Embodiment three: palmatin hydrochloride is to the sick test of field control of bacterial blight of rice
1. for examination material:
Strains tested: Xanthomonas oryzae strong toxic strain FUJ, the molten bacillus strain YFY 02 of antibiotic; For test preparation: palmatin hydrochloride preparation, 1% Shen piperazine mycin suspending agent, 1000 times of liquid, the peaceful 100 times of liquid of tikka; For examination medium: KB medium, NA medium; For studying thing: landlord transplants rice seedlings, and kind moral is excellent 13, red excellent No. 4 of bacterial leaf spot disease-resistant variety; Controlling object: bacterial blight of rice;
Experiment place: rice test field, Dehong prefecture Ruili City, Yunnan Province, middle fertility, plantation landlord transplant rice seedlings kind-De excellent 13, and community is single file district, area 30m
2, each process establishes 3 repetitions, randomized arrangement, totally 9 communities, and surrounding establishes protection row, and red excellent No. 4 of protection behavior bacterial leaf spot disease-resistant variety, by the standardized management of high-grade rice cultivation step.
2 test methods
2.1 bacterial blight of rice field controling tests
The preparation of Xanthomonas oryzae FUJ bacteria suspension: after leaf spot bacteria FUJ is activated 48h, be transplanted to the flat lining out of NA and cultivate 48h (28 DEG C), pathogen is made bacteria suspension with under aseptic washing from solid culture medium, shaking table shakes up, with spectrophotometer, bacteria suspension cell concentration is equivalent to 3 × 10
8cfu/mL, for inoculation.
Process one: antibiotic molten bacillus YFY02 secondary metabolites palmatin hydrochloride preparation spray inoculation (booting stage) on rice leaf by concentration being first 1ug/mL, inoculum concentration is 100mL/ strain, next day Inoculated Rice leaf spot bacteria FUJ bacteria suspension, inoculum concentration is 100mL/ strain; Process two: only water receiving rice bacterial leaf spot pathogenic bacteria FUJ bacteria suspension, inoculum concentration is 100mL/ strain; Contrast: using sterile water spray inoculation paddy rice as blank, inoculum concentration is 100mL/ strain; Each process arranges 3 repetitions, inoculates 21 days " Invest, Then Investigate "s, investigates the disease number of sheets, and sick leaf-size class value, total number of sheets, calculate the incidence of disease, disease index.
Investigation record and severity Scaling standard as follows:
0 grade: immunity, clip only has scar without scab;
1 grade: lesion area accounts for less than 10% of leaf area;
3 grades: lesion area accounts for about 1/4 of leaf area;
5 grades: lesion area accounts for about 1/2 of leaf area;
7 grades: lesion area accounts for about 3/4 of leaf area;
9 grades: full leaf morbidity.
Incidence of disease %=morbidity strain (leaf) number × 100/ total strain of investigation (leaf) number
Disease index=[Σ (diseased plant at different levels (leaf) number × this sick level value)/investigate total strain (leaf) number × highest sick value of series] × 100%
Relative control effect %=(contrast disease index-process disease index) × 100/ contrast disease index.
2.2 bacterial blight of rice field efficacy measurement results
Palmatin hydrochloride shows the measurement result of bacterial blight of rice field efficacy: palmatin hydrochloride can be stablized, inhibit the generation of bacterial blight of rice efficiently, the incidence of disease, disease index comparatively control group all significantly reduce, preventive effect, up to 55.9%, shows the application and development prospect (table 5) that it is potential.
Table 5 palmatin hydrochloride measures the field efficacy of bacterial blight of rice
Embodiment four: palmatin hydrochloride is to the sick test of field control of Cruciferae club root
1. for examination material:
Strains tested: Brassica genus pathogen plasmodiophora (Plasmodiophora brassicae Woron.), the molten bacillus strain YFY 02 of antibiotic; For test preparation: palmatin hydrochloride preparation, section's good (cyazofamid) 2000 times of liquid; For studying thing: Chinese cabbage susceptible variety Qingdao 83-1 (the international seedling Co., Ltd in Qingdao), No. 3, the disease-resistant variety Chinese People's Anti-Japanese Military and Political College; Controlling object: Cruciferae club root;
Experiment place: Chinese cabbage experimental field, Chuxiong Prefecture Lufeng County of Guangdong Province, Yunnan Province, middle fertility, plantation breeds of Chinese cabbage Qingdao 83-1 (high sense club root), area 30m
2, each process establishes 3 repetitions, randomized arrangement, totally 9 communities, and community is single file district, often row 14 cave, 3, every cave seedling, totally 42 young plants, and surrounding establishes protection row, protection row plantation anti-club root kind--No. 3, the Chinese People's Anti-Japanese Military and Political College.
2 test methods
2.1 Cruciferae club root field controling tests
Club root natural occurrence field, field is selected to carry out club root controlling experiment; After sowing Chinese cabbage seed, 7d, 14d and 21d carry out root irrigation, and every cave Chinese cabbage seedling fills with the palmatine preparation that 100mL prepares; Sample in disease occurrence in peak period (after inoculation 45d), each sampling 20 strains, investigate and record the physical signs such as each kind plant height, leaf area, plant fresh weight and morbidity grade, statistics club root incidence and disease index, analysis result.
Investigation record and severity Scaling standard as follows:
0 grade: root is without tumour, and root system development is normal;
The root of 1 grade: 1% ~ 30% raw a little tumour, has little nodositas lump, does not mutually connect;
The root of 2 grades: 31% ~ 60% raw tumour, has large nodositas or spherical lump, is interconnected;
The root of 3 grades: 61% ~ 100% raw tumour, and expand to formation spindle-like knurl, large knurl block extends to hypocotyl, and plant is short and small, stops growing.
Disease index=[Σ (diseased plant number × relative level numerical value at different levels)/highest level × investigate total strain number] × 100%
Disease index <10 is disease-resistant, and disease index >=10 are susceptible
The incidence of disease=(morbidity strain number/investigate total strain number) × 100%
Relative control effect=(contrast disease index-process disease index)/contrast disease index × 100%
2.2 Cruciferae club root field efficacy measurement results
Palmatin hydrochloride shows the measurement result of Cruciferae club root field efficacy: palmatin hydrochloride can be stablized, inhibit the generation of Cruciferae club root efficiently, the incidence of disease, disease index comparatively control group all significantly reduce, preventive effect, up to 55.8%, shows the application and development prospect (table 6) that it is potential.
Table 6 palmatin hydrochloride measures the field efficacy of Cruciferae club root
Claims (2)
1. a microbial secondary metabolite palmatin hydrochloride, is characterized in that this secondary metabolite palmatin hydrochloride obtains through following steps:
A. producing bacterial strain is the molten bacillus of antibiotic (Lysobacter antibioticus) YFY02, preserving number: CGMCC NO.2403;
B. utilize KB medium culture bacterial strain YFY02 two days later, adopt common fermentation processes to carry out expansion and cultivate, fermentation liquor is obtained cometabolism crude extract by carrying out rotary evaporation after extraction into ethyl acetate;
C. utilize the spectroscopic technique such as liquid chromatography, Sephadex LH-20 gel filtration chromatography, infrared spectrum, ultraviolet spectra, optically-active, mass spectrum, nuclear magnetic resonnance, cometabolism crude extract is carried out being separated, purifying and identifying;
D. effective secondary metabolite qualification result of antibiotic molten bacillus strain YFY02 is: 2,3,9,10-tetramethoxy-5,13a-dihydro-6H-isoquino, i.e. palmatin hydrochloride (Palmatine chloride), molecular formula is: C21H20NO4, and chemical structural formula is as follows:
2. microbial secondary metabolite palmatin hydrochloride according to claim 1 is preparing the application in water prevention rice bacterial leaf spot pathogenic bacteria, Population of Xanthomonas Oryzae Pv bacterium, Cruciferae pathogen plasmodiophora preparation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106478622A (en) * | 2016-08-27 | 2017-03-08 | 广东罗浮山国药股份有限公司 | A kind of compound extracting from Tinospora sinensis and preparation method thereof |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
| CN112715561A (en) * | 2020-12-30 | 2021-04-30 | 湖南农业大学 | Compound with synergistic effect on shenqinmycin, composition and application thereof |
-
2014
- 2014-11-13 CN CN201410639916.1A patent/CN104561179A/en active Pending
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| GEORGE P TEGOS: "Natural substrates and inhibitors of multidrug resistant pumps (MDRs) redefine the plant antimicrobials", 《NATURALLY OCCURRING BIOACTIVE COMPOUNDS》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106478622A (en) * | 2016-08-27 | 2017-03-08 | 广东罗浮山国药股份有限公司 | A kind of compound extracting from Tinospora sinensis and preparation method thereof |
| CN106478622B (en) * | 2016-08-27 | 2018-12-21 | 广东罗浮山国药股份有限公司 | A kind of compound and preparation method thereof extracted from Tinospora sinensis |
| CN110352958A (en) * | 2019-08-23 | 2019-10-22 | 云南农业大学 | A kind of Bio-seed Coating agent and preparation method thereof |
| CN112715561A (en) * | 2020-12-30 | 2021-04-30 | 湖南农业大学 | Compound with synergistic effect on shenqinmycin, composition and application thereof |
| CN112715561B (en) * | 2020-12-30 | 2022-02-15 | 湖南农业大学 | Compound with synergistic effect on shenqinmycin, composition and application thereof |
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Application publication date: 20150429 |
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| RJ01 | Rejection of invention patent application after publication |