CN102010841B - Functional bacterial strain No.5 as well as cultivation method and application of bacterial preparation - Google Patents
Functional bacterial strain No.5 as well as cultivation method and application of bacterial preparation Download PDFInfo
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- CN102010841B CN102010841B CN 201010267109 CN201010267109A CN102010841B CN 102010841 B CN102010841 B CN 102010841B CN 201010267109 CN201010267109 CN 201010267109 CN 201010267109 A CN201010267109 A CN 201010267109A CN 102010841 B CN102010841 B CN 102010841B
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- microbial inoculum
- function stem
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Abstract
The invention discloses a functional bacterial strain No.5 as well as a cultivation method and the application of a bacterial preparation. The functional bacterial strain is preserved and stored in the CGMCC (China General Microorganism Conservation Center), has a register number of CGMCC No.3938 and a Latin scientific name of Pseudomonas brassicacearum. The functional bacterial strain can be used for promoting the growth of seedlings, improving the yield and the quality of tea and the fruit quality, resisting pathogens, decomposing inorganic and organic phosphorus into soluble phosphorus, improving the soil fertility and obviously improving the soil quality and can also grow in a culture medium of 7% NaCl.
Description
Technical field
The present invention relates to a kind of function stem, relate in particular to a kind of function stem No. five and the application of cultural method and microbial inoculum.
Background technology
Because life-time service chemical fertilizer and agricultural chemicals cause problems such as soil compaction, land deterioration, chemical fertilizer and pesticide residue easily, bio-feritlizer is developed rapidly, is used to promote seedling growth, anti-microbial pathogen, improves edatope, aspect such as build up fertility.
In the prior art, there are problems such as function singleness, quality instability, soil suitability difference in bio-feritlizer.
Summary of the invention
The purpose of this invention is to provide the good function stem No. five of a kind of complete function, steady quality, soil suitability and the application of cultural method and microbial inoculum thereof.
The objective of the invention is to realize through following technical scheme:
Function stem of the present invention No. five, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3938;
The Latin formal name used at school is: Pseudomonas brassicacearum.
The cultural method that above-mentioned function stem of the present invention is No. five is characterized in that this function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, this microbial inoculum comprise above-mentioned function stem No. five, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention:
This microbial inoculum is used to promote seedling growth;
This microbial inoculum is used for anti-microbial pathogen;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, the sugar degree of one or more fruit below improving: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
Technical scheme by the invention described above provides can be found out, function stem of the present invention No. five, can be used to promote seedling growth, anti-microbial pathogen, with insoluble inorganic phosphorus and organophosphorus be decomposed into titanium pigment, improve fruit quality, build up fertility etc.Complete function, steady quality, soil suitability are good.
Preservation information
Conserving microorganism (strain) classification name: the preservation center does not provide Chinese classification name as yet, claims temporarily: function stem No. five;
Conserving microorganism (strain) Latin formal name used at school: Pseudomonas brassicacearum;
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC);
Depositary institution address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica;
Preservation date: on June 22nd, 2010;
Deposit number: CGMCC No.3938.
Embodiment
Function stem of the present invention No. five, its preferable embodiment is:
This function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3938;
The Latin formal name used at school is: Pseudomonas brassicacearum.
This function stem grows in 7% NaCl substratum.
Microbial inoculum of the present invention, its preferable embodiment be, this microbial inoculum comprises above-mentioned function stem No. five, and this microbial inoculum is solid or liquid bacterial agent.
The application of above-mentioned microbial inoculum of the present invention comprises following one or more application:
This microbial inoculum is used to promote seedling growth, such as being used for promoting following one or more seedling growths: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves; This microbial inoculum can also be used for improving the content that tealeaves has the tea-polyphenol of anti-cancer function;
This microbial inoculum is used for anti-microbial pathogen, is used to prevent and treat the generation of continuous cropping watermelon blight such as this microbial inoculum;
This microbial inoculum is used for insoluble inorganic phosphorus, organophosphorus are decomposed into titanium pigment;
This microbial inoculum is used to improve the quality of fruit, such as the sugar degree that is used to improve following one or more fruit: apple, oranges and tangerines, pears, peach;
This microbial inoculum and chemical fertilizer mix and execute, and are used to build up fertility.
The screening process of function stem of the present invention:
The applicant is in the soil quality evolution research process to artificial forest such as China fir, willow, Pinus massoniana Lamb, eucalyptus; To being positioned at the main reproducting tree species under different terrain, the different soils land occupation condition, under the different stand type; Accomplished the investigation of 1000 polylith temporary sample plots and permanent sample plot; Meanwhile, the secondary forest under the different land occupation conditions, under the different stand type, primary forest have also been done a large amount of appearance ground investigation, in the research process; Soil microflora to above-mentioned every research appearance ground; Done corresponding probe research, found nocardia kind distribution, particularly the dominant population regularity of distribution on these research appearance ground, and appeared closely related with the soil fertility and the increase in standing timber.
On the basis of above-mentioned research, do function yeast screening: to the dominant genera kind separate, purifying and biological characteristic research, and expand numerous, germination test, seedbed test, find that these bacterial strains all have the function of obvious promotion seedling growth.
Further do disease-resistant test, find that these bacterial strains have anti-microbial pathogen function in various degree, applicant's Pang Guo village evidence in the Daxing can effectively prevent and treat the generation of continuous cropping watermelon blight.
Do the test of anti-inorganic salt and show, find that these bacterial strains can grow in 7% NaCl substratum.Do the insoluble phosphorus decomposition run, find that these bacterial strains have the insoluble of decomposition inorganic phosphorus, organophosphorus is the function of titanium pigment.
The applicant produces solid and the liquid bacterial agent of developing these bacterial strains through a large amount of for many years indoor and outdoor tests.
Application effect:
On main reproducting tree species such as eucalyptus, Pinus massoniana Lamb and willow, use; Obvious effect of increasing production improves the increase in standing timber, generally more than 10%; Use seedling stage; Increase effect and reach more than the 20%-30%, have tangible growth promoting function, seedling stage to use the growth effect to ornamental tree species like happy eastern lily magnolia, sweet osmanthus etc. and reach more than the 20%-30%.Be applied to economic forest seeds such as tealeaves, increasing degree reaches 30-40%, and moreover, improving effectively has the anti-cancer function polyphenol content in the tealeaves.Improve the quality of fruit, improve the sugar degree of apple, oranges and tangerines, pears and peach.
The biomaterial characteristic that function stem of the present invention is No. five:
Referring to: the check probation report that Institute of Microorganism, Academia Sinica provides, the detection probation report about No. 5 bacterial strains wherein are the biomaterial characteristic of function stem of the present invention No. five.
The above; Be merely the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technician who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (4)
1. a function stem is No. five, it is characterized in that, this function stem is preserved by China Committee for Culture Collection of Microorganisms's common micro-organisms center collection, and registering on the books is numbered: CGMCCNo.3938;
The Latin formal name used at school is: Pseudomonas brassicacearum.
2. the cultural method of the described function stem of claim 1 No. five is characterized in that this function stem grows in 7% NaCl substratum.
3. a microbial inoculum is characterized in that, this microbial inoculum comprises the described function stem of claim 1 No. five, and this microbial inoculum is solid or liquid bacterial agent.
4. the application of the described microbial inoculum of claim 3 is characterized in that, this microbial inoculum is used to promote seedling growth, said nursery stock be following any: eucalyptus, Pinus massoniana Lamb, willow, happy eastern lily magnolia, sweet osmanthus, tealeaves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010267109 CN102010841B (en) | 2010-08-27 | 2010-08-27 | Functional bacterial strain No.5 as well as cultivation method and application of bacterial preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010267109 CN102010841B (en) | 2010-08-27 | 2010-08-27 | Functional bacterial strain No.5 as well as cultivation method and application of bacterial preparation |
Publications (2)
| Publication Number | Publication Date |
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| CN102010841A CN102010841A (en) | 2011-04-13 |
| CN102010841B true CN102010841B (en) | 2012-05-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010267109 Expired - Fee Related CN102010841B (en) | 2010-08-27 | 2010-08-27 | Functional bacterial strain No.5 as well as cultivation method and application of bacterial preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333843A (en) * | 2013-07-19 | 2013-10-02 | 上海农乐生物制品股份有限公司 | Pseudomonas brassicacearum and cultivation method and application thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477164A (en) * | 2011-09-30 | 2012-05-30 | 深圳光启高等理工研究院 | Preparation method for inhomogeneous dielectric substrate |
| CN103329907B (en) * | 2013-07-22 | 2016-01-20 | 上海农乐生物制品股份有限公司 | A kind of preparation method of fen second mycin biological bactericide |
| CN109182199B (en) * | 2018-09-29 | 2021-09-07 | 中国科学院成都生物研究所 | Pseudomonas brassicae with plant growth promoting effect |
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2010
- 2010-08-27 CN CN 201010267109 patent/CN102010841B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333843A (en) * | 2013-07-19 | 2013-10-02 | 上海农乐生物制品股份有限公司 | Pseudomonas brassicacearum and cultivation method and application thereof |
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| Publication number | Publication date |
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| CN102010841A (en) | 2011-04-13 |
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