CN101955399A - Special liquid culture medium for strains and using method - Google Patents
Special liquid culture medium for strains and using method Download PDFInfo
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- CN101955399A CN101955399A CN201010291375XA CN201010291375A CN101955399A CN 101955399 A CN101955399 A CN 101955399A CN 201010291375X A CN201010291375X A CN 201010291375XA CN 201010291375 A CN201010291375 A CN 201010291375A CN 101955399 A CN101955399 A CN 101955399A
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Abstract
The invention discloses a special liquid culture medium for strains and a using method. Each solid component of the culture medium is prepared and mixed in advance, and liquid components are added when the culture medium is used, wherein the prepared culture medium comprises the following components per liter: 15 to 20g of starch, 15 to 20g of soybean meal, 15 to 20g of glucose, 2 to 5g of potassium dihydrogen phosphate, 1 to 1.5g of magnesium sulfate, 2 to 3g peptone and 0.3 to 0.5ml of antifoaming agent. The using method comprises the following steps of: stirring the culture medium with a small amount of clean water to form paste; weighing hot water at the temperature of between 80 and 85 DEG C in an amount which is 2 to 3 times the total weight of a solid culture medium, dissolving the solid culture medium with stirring, and then adding the antifoaming agent; adding the hot water into an incubator to prepare diluted solution and adding sufficient hot water to fix the volume; and after high temperature sterilization, cooling the diluted solution below the temperature of 28 DEG C to inoculate. The culture medium has the advantages of rich and full nutrients, quick absorption of hyphae, high activity of strains, no demixing during cultivation, high concentration of fungus balls, a large number of inoculated bags, convenient storage and transportation of products, time and labor-saving application, safety, high efficiency, high quality and capacity of greatly reducing labor intensity and the production cost of the strains.
Description
Technical field
The present invention relates to the substratum of a kind of edible mushrooms, liquid spawn special culture media and using method that particularly a kind of edible fungus industrial is produced.
Background technology
Solid spawn is used in the original production of edible mushrooms usually, adopts the liquid fungus seed culture technique in recent years.The conventional practice of liquid spawn culture medium is: A cuts into slices potato, is put into heated and boiled in the water with wheat bran, and potato boils to crisp and filter when mashed; B adds potassium primary phosphate in the solution, treats to dissolve altogether the back and adds sal epsom; C is with brown sugar, glucose, and peptone adds in the solution; D adds vitamins B
1E adds defoamer.Also there are a lot of problems in this bacterial classification that is used for the liquid of Edible Fungi: not of uniform size the causing of liquid spawn bacterium ball of (1) liquid spawn culture medium production, and skewness is unfavorable for the growth of bacterium ball, bacterium ball precipitation, easily aging in the middle of substratum; Female kind breeding time is longer, and mycelia is of low quality; (2) contain compositions such as a large amount of glucose, sucrose in the used substratum of liquid spawn, the nutrient media components that uses in the production culturing process is various, and production cost is higher, also gives to produce and operation is made troubles; (3) the nutrient media components places of origin of raw materials that has, quality difference influence seed production quality; (4) preparation is wasted time and energy, the labour intensity height, and storing is inconvenient.
Summary of the invention
Technical problem to be solved by this invention is: a kind of cost is low, nutritious, the simple edible fungus industrial of preparation technology is produced liquid spawn special culture media and using method thereof are provided.The present invention is achieved like this:
A kind of liquid spawn special culture media is characterized in that: each solid constituent of substratum is prepared mixing in advance, add liquid components during use again, the content of each component is in the every liter of substratum in preparation back:
Starch 15～20g, analysis for soybean powder 15～20g, glucose 15～20g, potassium primary phosphate 2～5g, sal epsom 1～1.5g, peptone 2～3g, defoamer 0.3～0.5ml.
The using method of substratum of the present invention comprises the steps:
(1) it is standby to take by weighing preparation substratum each solid constituent in advance by above-mentioned amount, adds a spot of clear water stirring and dissolving during use earlier and becomes pasty state;
(2) get the hot water of 80～85 ℃ of 2～3 times of solid medium gross weights amounts,, stir and do not have caking, add defoamer in the matrix after dissolving and defoam the pasty state stromatolysis of step (1);
(3) step (2) is made culture medium solution and is injected in 80～85 ℃ of hot water of strain cultivation device and makes diluting soln, and with clear water supply be settled to volume required;
(4) sterilize in 120～125 ℃ and keep inoculating below 30～35 minutes postcooling to 28 ℃, cultivate and observe bacterium ball concentration and reach more than 95%, size does not evenly precipitate, and bacterium liquid is white in color and gets final product.
In order not influence sterilising effect, the culture medium solution after step (2) dissolving is if any caking or particle, available 4～6 layers of filtered through gauze; Pei Zhi solid medium need place 5～10 ℃ of sealing air seasonings to store 3 months quality guaranteed perioves in advance.
Liquid spawn special culture media of the present invention adopts modern biotechnology refinement, scientific matching, screening and optimizing, and formulated liquid spawn produces required optimization matrix.This substratum is nutritious comprehensively, and that mycelia absorbs is fast, spawn activity is strong, it is not stratified to cultivate, bacterium ball concentration height, to connect bag quantity big; The proterties of product of the present invention is beige, and is Powdered, solid form, and storing is convenient; It is time saving and energy saving to use this substratum, safety, efficient, quality is good, can reduce the production cost of labour intensity and liquid spawn greatly.The comparative benefits of product of the present invention and traditional liquid bacterium culture medium is as follows:
1. save time: traditional substratum Production Time must need about 4 hours, and the liquid spawn special culture media only needs 20 minutes;
2. less manpower: traditional substratum needs 2 workmans, and special culture media is made and only needed 1 people;
3. reduce cost: it is that example needs 120 yuans with 70 jars that traditional fabrication needs cost for one jar, and special culture media only needs 70 yuans now.
4. improve the quality: the liquid spawn bacterium ball size that the dedicated liquid bacterium culture medium is produced is consistent, uniform distribution.In the middle of substratum, the bacterium ball does not precipitate, and is difficult for wearing out, and meets the strain quality standard.
5. conformance with standard: potato that traditional substratum is used and wheat bran skin and other places of origin of raw materials are different, and quality also differs.For example: each position starch content difference of potato influence seed production quality, and the special-purpose same standard of bacterium culture medium of planting does not have regional difference.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
One, preparation weight
Use full standing grain bacterium industry CQY70 strain cultivation to produce the black fungus bacterial classification, its substratum dosage is:
Starch 675g, analysis for soybean powder 675g, glucose 675g, sal epsom 45g, peptone 90g, potassium primary phosphate 90g, bubble enemy 13.5ml.
Two, using method
1. according to the model of selecting for use of strain cultivation device, press incubator Calculation of Effective Volume substratum consumption; Prewired solid medium storage procedures: Pei Zhi solid medium need place sealing air seasoning place, store in 5～10 ℃ of temperature in advance, to avoid the humidity plague of rats of damaging by worms, 3 months quality guaranteed perioves.
2, it is standby to take by weighing preparation substratum each solid constituent in advance by above-mentioned amount, adds a spot of clear water stirring and dissolving during use earlier and becomes pasty state;
3, take by weighing 80～85 ℃ of hot water of 3 times of amounts of solid medium gross weight,, stir and do not have caking, add defoamer in the matrix after dissolving and defoam the pasty state stromatolysis of step 2; Liquid nutrient medium after the dissolving is if any caking or particle, and available 4～6 layers of husky cloth filter, otherwise influence sterilising effect.
4, add water 30L in volume is the strain cultivation device of 45L and heat up, when water temperature reached 80～85 ℃, the culture medium solution that step 3 is made was injected in the incubator mixing and makes diluting soln, and supplied with clear water and to be settled to 45L;
5, sterilize in 120～125 ℃ and keep inoculating below 30～35 minutes postcooling to 28 ℃, cultivate and observe bacterium ball concentration and reach more than 95%, size does not evenly precipitate, and bacterium liquid is white in color and gets final product.
Embodiment 2:
Use full standing grain bacterium industry CQY70 strain cultivation to produce the black fungus bacterial classification, its substratum dosage is:
Starch 765g, analysis for soybean powder 765g, glucose 765g, sal epsom 54g, peptone 112.5g, potassium primary phosphate 180g, bubble enemy 18ml.
Using method is with embodiment 1.
Embodiment 3:
Use full standing grain bacterium industry CQY70 strain cultivation to produce the black fungus bacterial classification, its substratum dosage is:
Starch 810g, analysis for soybean powder 810g, glucose 810g, sal epsom 67.5g, peptone 135g, potassium primary phosphate 225g, bubble enemy 22.5ml.
Using method is with embodiment 1.
Claims (4)
1. liquid spawn special culture media is characterized in that: each solid constituent of substratum is prepared mixing in advance, add liquid components during use again, the content of each component is in the every liter of substratum in preparation back:
Starch 15～20g, analysis for soybean powder 15～20g, glucose 15～20g, potassium primary phosphate 2～5g, sal epsom 1～1.5g,
Peptone 2～3g, defoamer 0.3～0.5ml.
2. the using method of liquid spawn special culture media according to claim 1 is characterized in that comprising the steps:
(1) it is standby to take by weighing preparation substratum each solid constituent in advance by above-mentioned amount, adds a spot of clear water stirring and dissolving during use earlier and becomes pasty state;
(2) get the hot water of 80～85 ℃ of 2～3 times of solid medium gross weights amounts,, stir and do not have caking, add defoamer in the matrix after dissolving the pasty state stromatolysis of step (1);
(3) step (2) is made culture medium solution and is injected in 80～85 ℃ of hot water of strain cultivation device and makes diluting soln, and with clear water supply be settled to volume required;
(4) sterilize in 120～125 ℃ and keep inoculating below 30～35 minutes postcooling to 28 ℃, cultivate and observe bacterium ball concentration and reach more than 95%, size does not evenly precipitate, and bacterium liquid is white in color and gets final product.
3. the using method of liquid spawn special culture media according to claim 1, the culture medium solution after it is characterized in that dissolving is if any caking or particle, with 4～6 layers of filtered through gauze.
4. the using method of liquid spawn special culture media according to claim 1 is characterized in that the solid medium of preparing in advance places 5～10 ℃ of sealing air seasonings to store 3 months quality guaranteed perioves.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533883A (en) * | 2011-12-23 | 2012-07-04 | 山东宝莫生物化工股份有限公司 | Preheating method of water for fermentation tank charging |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN107805096A (en) * | 2017-10-25 | 2018-03-16 | 翔天农业开发集团股份有限公司 | A kind of preparation method of edible fungus liquid culture growth medium |
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| CN1724640A (en) * | 2005-06-13 | 2006-01-25 | 浙江大学 | Universal edible fungus liquid culture growth medium and its manufacturing technology |
| CN1888048A (en) * | 2006-07-21 | 2007-01-03 | 江南大学 | Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source |
| CN101041808A (en) * | 2007-02-28 | 2007-09-26 | 上海浦东天厨菇业有限公司 | Golden mushroom factory-production of liquid bacterial culture medium and preparation method thereof |
| CN101195806A (en) * | 2007-12-04 | 2008-06-11 | 西宁市城西区食用菌研究所 | Industrial production method of cordyceps mushroom |
| CN101591618A (en) * | 2008-05-28 | 2009-12-02 | 中国科学院微生物研究所 | A kind of Xylaria gracillima strain and liquid fermentation culturing method thereof and application |
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2010
- 2010-09-20 CN CN201010291375XA patent/CN101955399A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724640A (en) * | 2005-06-13 | 2006-01-25 | 浙江大学 | Universal edible fungus liquid culture growth medium and its manufacturing technology |
| CN1888048A (en) * | 2006-07-21 | 2007-01-03 | 江南大学 | Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source |
| CN101041808A (en) * | 2007-02-28 | 2007-09-26 | 上海浦东天厨菇业有限公司 | Golden mushroom factory-production of liquid bacterial culture medium and preparation method thereof |
| CN101195806A (en) * | 2007-12-04 | 2008-06-11 | 西宁市城西区食用菌研究所 | Industrial production method of cordyceps mushroom |
| CN101591618A (en) * | 2008-05-28 | 2009-12-02 | 中国科学院微生物研究所 | A kind of Xylaria gracillima strain and liquid fermentation culturing method thereof and application |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533883A (en) * | 2011-12-23 | 2012-07-04 | 山东宝莫生物化工股份有限公司 | Preheating method of water for fermentation tank charging |
| CN103242080A (en) * | 2013-05-20 | 2013-08-14 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN103242080B (en) * | 2013-05-20 | 2014-08-13 | 威海鑫宝食品有限公司 | Mushroom liquid strain as well as preparation method, preparation device and application thereof |
| CN107805096A (en) * | 2017-10-25 | 2018-03-16 | 翔天农业开发集团股份有限公司 | A kind of preparation method of edible fungus liquid culture growth medium |
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Application publication date: 20110126 |