CN101940631A - Method for preparing pollen itching-dispelling pain-relieving tincture - Google Patents
Method for preparing pollen itching-dispelling pain-relieving tincture Download PDFInfo
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Abstract
The invention discloses a method for preparing pollen itching-dispelling pain-relieving tincture, which is characterized in that: the tincture is prepared from 45 grams of mongolian snakegourd root, 45 grams of dahurian angelica root, 34 grams of short-pedicel aconite root, 4 grams of borneol, 5 grams of camphor, 20 grams of frankincense, 20 grams of myrrh, 10 milliliters of mint oil, 5 milliliters of eucalyptus oil and 5 milliliters of glycerol. By repeated test and comparison, the method for preparing the pollen itching-dispelling pain-relieving tincture can improve the treatment effect of the medicament.
Description
Technical field
The present invention relates to a kind of preparation method of pollen dispel itch analgesic tincture, belong to field of pharmaceutical technology.
Background technology
We come from ethnic groups medication among the people, the strong analgesic activity of getting Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) is a principal agent, be aided with the Radix Angelicae Dahuricae, Radix Trichosanthis is a ministerial drug, tool detumescence, itching relieving effect, help Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) that good antiinflammatory, pain relieving effect are arranged, pain is then obstructed, and general rule is not bitterly, Gu Zuo is to have the Olibanum of blood circulation promoting and blood stasis dispelling, promoting the circulation of QI to relieve pain, Myrrha.We are used for blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, dispelling wind for relieving itching.The applicant furthers investigate this medicine production technology, can improve the therapeutic effect of medicine.
Summary of the invention
The objective of the invention is to, provide a kind of preparation method that is used for the treatment of mosquito bite, traumatic injury medicine, particularly a kind of preparation method of pollen dispel itch analgesic tincture.To optimize pharmaceutical preparation, improve therapeutic effect.
Technical scheme of the present invention.The preparation method of pollen dispel itch analgesic tincture, get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten flavors, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, add 1～3 times of amount 80% ethanol and soaked into 24 hours, 4～6 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 6～8 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, add 1～2 times of water gaging, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8～9 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 4～6 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 1～3 times of amount petroleum ether extraction 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 1～3 times of amount chloroform extraction 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, promptly.
The preparation method of above-mentioned pollen dispel itch analgesic tincture, get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten flavors, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, add 2 times of amount 80% ethanol and soaked into 24 hours, 5 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 7 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, add 1.5 times of water gagings, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8.5 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 5 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 2 times of amount petroleum ether extractiones 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, promptly.
By weight, add 4% azone and 2% ethyl cellulose in the above-mentioned supernatant.
The applicant sums up and draws the new preparation method of pollen dispel itch analgesic tincture by repetition test, contrast, adopts this method can improve the therapeutic effect of medicine.Below by test the example further set forth preparation method of the present invention beneficial effect:
One, the extracting method of preferred Olibanum, Myrrha can strengthen drug effect
Olibanum and Myrrha are pollen dispel itch analgesic tincture prescription Chinese crude drug, and two medicines all have the effect of promoting blood circulation and stopping pain, mainly contain volatile oil, resin and natural gum.After deliberation, Olibanum and Myrrha ethanol extraction have anti-inflammatory activity, and volatile oil has analgesic activity.Pollen dispel itch analgesic tincture has blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, dispelling wind for relieving itching.Be used for mosquito bite, traumatic injury, soft tissue contusion.In order to improve the therapeutic effect of these product, the applicant tests the extraction process of Olibanum and Myrrha:
Experimental technique and data are as follows:
Extracting method to Olibanum and Myrrha:
1, ethanol extract: measure Olibanum and Myrrha is ground into coarse powder by prescription, mixing adds 2 times of amount 80% ethanol and soaked into 24 hours, and 5 times of amounts of reuse, 80% ethanol divides dipping three times, and 3 hours for the first time, second and third time each 2 hours merged three times impregnation liquid, and filtration gets filtrate.
2, volatile oil: measure Olibanum and Myrrha is ground into coarse powder by prescription, water vapour extracts volatile oil.
3, ethanol extract+volatile oil: measure Olibanum and Myrrha is ground into coarse powder by prescription, water vapour extracts volatile oil, and medicinal residues divide dipping 2 times with 7 times of amount 95% ethanol, and each 3 hours, must impregnation liquid, with impregnation liquid and volatile oil mixing.
Analgesic activity (writhing method) test: get 50 of mices, male and female half and half, be divided into 5 groups after weighing at random, i.e. normal control group (normal saline 20ml/kg), ethanol extract (0.1g/kg), volatile oil (0.1g/kg), ethanol extract+volatile oil (0.1g/kg) and positive controls (rotundine 0.045g/kg).Every day gastric infusion once, continuous 5 days.1h lumbar injection 0.6% acetum 0.01ml/g after the last administration, observe occur in the 10min turn round the body number of times.
The result shows, ethanol extract+volatile oil gastric infusion can suppress the mouse writhing reaction due to the acetic acid, turns round the body number of times and obviously reduces, and with the matched group comparing difference statistical significance (P＜0.05 is arranged, see Table 1) illustrate and can strengthen analgesic effect by the extracting method that on original technology, changes Olibanum, Myrrha.
Olibanum, Myrrha extract analgesic activity (writhing method)
Annotate: compare with normal control
*P＜0.05
*P＜0.01
Draw through test, the extracting method of preferred Olibanum, Myrrha is ethanol extract+volatile oil, can strengthen drug effect.Concrete extraction process is: measure Olibanum and Myrrha is ground into coarse powder by prescription, water vapour extracts volatile oil, and medicinal residues are measured 95% ethanol with 7 times and divided dipping, each 3 hours 2 times.Impregnation liquid and volatile oil add in the filtrate of other medicines, and mixing left standstill 24 hours, get supernatant and filter.
Two, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani)
Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) is a pollen dispel itch analgesic tincture prescription Chinese crude drug, widely the traumatic injury of folklore and use, the analgesic of curing the wound.The main effective ingredient of Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) is an alkaloid, and its mesaconitine has analgesia, antiinflammatory action.Former technology in order to improve the therapeutic effect of these product, improves alkaloids content with 80% ethanol extraction effective ingredient, and the applicant tests the Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) extraction process.Experimental technique and data are as follows:
1,80% alcohol dipping method: take by weighing Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) fine powder 30g, add 300ml80% ethanol, dipping spends the night, pour out lixiviating solution, added 150ml80% ethanol again 3 hours, mixed impregnant liquor, reclaim ethanol, get concentrate, with high effective liquid chromatography for measuring sample mesaconitine extraction ratio.
2, acidic ethanol extraction method: take by weighing Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) fine powder 30g, add 300ml acidic ethanol (1% hydrochloric acid), dipping spends the night, and pours out lixiviating solution, added 150ml acidic ethanol dipping again 3 hours, mixed impregnant liquor, recovery section ethanol, transferring pH value with 2% sodium hydroxide solution is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle concentrate, with high effective liquid chromatography for measuring sample mesaconitine extraction ratio.
3, cellulase solution: take by weighing Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) fine powder 30g, add 45ml water, the control temperature is at 45 ℃, and transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio, after stirring in water bath is reacted 4 hours, add the 255ml95% alcohol dipping again and spend the night, pour out lixiviating solution, added the 150ml80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, adds 1% hydrochloric acid solution, transferring pH value is 2～3, add 2 times of amount petroleum ether extractiones 2 times, collect the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle concentrate, with high effective liquid chromatography for measuring sample mesaconitine extraction ratio.
Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) Different Extraction Method measurement result
| Extracting method | Aconitine extraction ratio % |
| 80% alcohol dipping method | 0.001675 |
| The acidic ethanol extraction method | 0.001752 |
| The cellulase solution | 0.002086 |
Through test, the cellulase solution of Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) can improve active constituent content, strengthens drug effect.Therefore, the extraction process of preferred Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) is: take by weighing Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) by prescription, add 1.5 times of water gagings, the control temperature is at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is carried out anti-4 hours, adds 8.5 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 5 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 2 times of amount petroleum ether extractiones 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle concentrate, add in the filtrate of other medicines, mixing left standstill 24 hours, got supernatant and filtered.
Three, adjuvant.Through test, in the tincture for preparing, add 4% azone and 2% ethyl cellulose, can improve the viscosity of tincture, reduce the volatility of medicine, can the holdup time of prolong drug on skin, be beneficial to the absorption of medicine.
Four, finished product optimize before the technology and optimize technology after efficacy result relatively:
(1), purpose: before and after investigating pollen dispel itch analgesic tincture optimization technology, the clinical observation of soft tissue contusion is carried out in the comparison of therapeutic effect.
(2), method: the product of getting it filled are applied in the affected part in right amount, slight massaging, every day 3 times, 7 days is a course of treatment, observes local pain, pruritus is improved situation, the record index of correlation.
(3), case choice criteria
1, the age: 18-65 year;
2, sex: the men and women does not limit;
3, do not merge organ diseases such as the heart, brain, liver, kidney;
4, based on acute soft tissue contusion.
(4), clinical diagnosis standard
1, diminishes sick and wounded history;
2, there are ecchymosis, swelling, pain in the part;
3, injured limb, function of joint have limited in various degree;
4, there is pressure pain point the damage part.
(5), curative effect determinate standard
1, cure: in the medication 5 days, pain and other transference cures, swelling is disappeared, and functional rehabilitation is normal;
2, produce effects: in the medication 5 days, pain and other symptoms disappear substantially, and swelling is obviously disappeared, and function is improved;
3, effective: in the medication 5 days, pain and other symptoms have and alleviate, and swelling has disappears, and it is normal that function is not recovered;
4, invalid: as relatively not have change after the medication before symptom and sign and the treatment.
(6), clinical data
This group is collected case and is had 108 examples, wherein with the finished product of optimizing technology (being called for short the treatment group) 58 examples, former technology finished product (abbreviation matched group) 50 examples, 120 examples are arranged during primary election, because of using other drug 4 examples, do not keep medication 8 examples and rejected, complete data person 108 examples (table 1, table 2) by rule.
Table 1 sex example (%)
Table 2 efficacy analysis example (%)
Two groups are relatively, statistical analysis P＞0.05, no significant difference, aspect acute soft tissue contusion, the finished product (being called for short the treatment group) of optimizing technology obviously is better than former technology finished product (abbreviation matched group), show optimize technology after therapeutic effect better.
The specific embodiment
Embodiments of the invention 1.The preparation method of pollen dispel itch analgesic tincture, get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten flavors, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, add 1～3 times of amount 80% ethanol and soaked into 24 hours, 4～6 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 6～8 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, add 1～2 times of water gaging, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8～9 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 4～6 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 1～3 times of amount petroleum ether extraction 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 1～3 times of amount chloroform extraction 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, promptly.
Embodiments of the invention 2: better scheme is: get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten the flavor, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing adds 2 times of amount 80% ethanol and soaked into 24 hours, 5 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours merged three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 7 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, the water that adds 1.5 times of amounts, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8.5 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 5 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 2 times of amount petroleum ether extractiones 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding an amount of 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, made 1000ml, carried out packing on request.
Function cures mainly
Seedling doctor: the triumphant repeated crust of rising sun loud, high-pitched sound, dimension resemble melt and lose and resemble, vast peace retaining Meng, the Chu home record card of dispelling.Suddenly: hilllock one is given, and the wheel official that receives wheel is washed.
The traditional Chinese medical science: blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, dispelling wind for relieving itching.Be used for mosquito bite, traumatic injury, soft tissue contusion.
Usage and dosage: this product external, get and smear the affected part in right amount, 3～4 times on the one.
Embodiments of the invention 3: the preparation method of pollen dispel itch analgesic tincture, get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten flavors, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, add 2 times of amount 80% ethanol and soaked into 24 hours, 5 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 7 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, the water that adds 1.5 times of amounts, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8.5 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 5 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 2 times of amount petroleum ether extractiones 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding an amount of 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate mixing, left standstill 24 hours, and got supernatant, in supernatant, add 4% azone and 2% ethyl cellulose, mixing is made 1000ml, carries out packing on request.
Claims (3)
1. the preparation method of a pollen dispel itch analgesic tincture, it is characterized in that: get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten the flavor, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, adding 1～3 times of amount 80% ethanol soaked into 24 hours, 4～6 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 6～8 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, add 1～2 times of water gaging, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8～9 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 4～6 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 1～3 times of amount petroleum ether extraction 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 1～3 times of amount chloroform extraction 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, promptly.
2. the preparation method of pollen dispel itch analgesic tincture according to claim 1, it is characterized in that: get Radix Trichosanthis 45g, Radix Angelicae Dahuricae 45g, Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) 34g, Borneolum Syntheticum 4g, Camphora 5g, Olibanum 20g, Myrrha 20g, Oleum menthae 10ml, Oleum Eucalypti 5ml, glycerol 5ml, more than ten the flavor, get Radix Trichosanthis, the Radix Angelicae Dahuricae is ground into coarse powder, mixing, adding 2 times of amount 80% ethanol soaked into 24 hours, 5 times of amounts of reuse, 80% ethanol divides dipping three times, 3 hours for the first time, second and third time each 2 hours, merge three times impregnation liquid, filter the gained filtrate for later use; Get Olibanum and Myrrha is ground into coarse powder, water steam extraction volatile oil, medicinal residues divide dipping, each 3 hours, impregnation liquid and volatile oil mixing for standby use 2 times with 7 times of amount 95% ethanol; Get Radix Aconiti Brachypodi (Radix Aconiti Szechenyiani) and be ground into fine powder, add 1.5 times of water gagings, the control temperature adds 1% hydrochloric acid solution at 45 ℃, transferring pH value is 4.5, the cellulase that adds the 7mg/g ratio after stirring in water bath is reacted 4 hours, adds 8.5 times of amount 95% alcohol dipping and spends the night, pour out lixiviating solution, added 5 times of amount 80% alcohol dipping again 3 hours, mixed impregnant liquor reclaims ethanol, the hydrochloric acid solution of adding 1%, transferring pH value is 2～3, adds 2 times of amount petroleum ether extractiones 2 times, collects the sour water layer, transferring pH value with strong aqua ammonia is 9～10, add 2 times of amount chloroform extractions 2 times, collect chloroform layer, handle to such an extent that concentrate is standby; After getting Borneolum Syntheticum, Camphora, Oleum menthae, Oleum Eucalypti, glycerol and adding 80% dissolve with ethanol, add in above-mentioned filtrate, the concentrate, mixing left standstill 24 hours, got supernatant, promptly.
3. the preparation method of pollen dispel itch analgesic tincture according to claim 1 and 2 is characterized in that: by weight, add 4% azone and 2% ethyl cellulose in the above-mentioned supernatant.
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| CN105997954A (en) * | 2016-06-24 | 2016-10-12 | 贵州弘康药业有限公司 | Itching-relieving and pain-relieving pollen liniment and preparation method thereof |
| CN105997855A (en) * | 2016-06-24 | 2016-10-12 | 贵州弘康药业有限公司 | Itching-relieving and pain-relieving pollen liniment and preparation method thereof |
| CN106074749A (en) * | 2016-06-27 | 2016-11-09 | 贵州弘康药业有限公司 | A kind of pollen is dispelled pain-easing spray and preparation method thereof of itching |
| CN106109609A (en) * | 2016-06-27 | 2016-11-16 | 贵州弘康药业有限公司 | A kind of pollen is dispelled analgesic gel agent and preparation method thereof of itching |
| CN106420973A (en) * | 2016-08-30 | 2017-02-22 | 中南民族大学 | Medicine composition for treating bee, scorpion and mosquito bites, and preparation and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105935379A (en) * | 2016-06-24 | 2016-09-14 | 贵州弘康药业有限公司 | Pollen itching and pain relieving ointment and manufacturing method thereof |
| CN105997954A (en) * | 2016-06-24 | 2016-10-12 | 贵州弘康药业有限公司 | Itching-relieving and pain-relieving pollen liniment and preparation method thereof |
| CN105997855A (en) * | 2016-06-24 | 2016-10-12 | 贵州弘康药业有限公司 | Itching-relieving and pain-relieving pollen liniment and preparation method thereof |
| CN106074749A (en) * | 2016-06-27 | 2016-11-09 | 贵州弘康药业有限公司 | A kind of pollen is dispelled pain-easing spray and preparation method thereof of itching |
| CN106109609A (en) * | 2016-06-27 | 2016-11-16 | 贵州弘康药业有限公司 | A kind of pollen is dispelled analgesic gel agent and preparation method thereof of itching |
| CN106420973A (en) * | 2016-08-30 | 2017-02-22 | 中南民族大学 | Medicine composition for treating bee, scorpion and mosquito bites, and preparation and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101940631B (en) | 2012-08-29 |
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Denomination of invention: Method for preparing pollen itching-dispelling pain-relieving tincture Effective date of registration: 20160115 Granted publication date: 20120829 Pledgee: Agricultural Bank Chinese Limited by Share Ltd Longli County branch Pledgor: Guizhou Hongkang Pharmaceutical Co., Ltd. Registration number: 2016520000001 |
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Denomination of invention: A preparation method of pollen antipruritic analgesic tincture Effective date of registration: 20210809 Granted publication date: 20120829 Pledgee: Agricultural Bank Chinese Limited by Share Ltd. Longli County branch Pledgor: GUIZHOU HONGKANG PHARMACEUTICAL Co.,Ltd. Registration number: Y2021520000010 |
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