CN101919485B - Method for extracting perilla protein nutrition powder from perilla cake - Google Patents
Method for extracting perilla protein nutrition powder from perilla cake Download PDFInfo
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- CN101919485B CN101919485B CN200910147929.6A CN200910147929A CN101919485B CN 101919485 B CN101919485 B CN 101919485B CN 200910147929 A CN200910147929 A CN 200910147929A CN 101919485 B CN101919485 B CN 101919485B
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Abstract
The invention relates to a method for extracting perilla protein nutrition powder from a perilla cake. The method comprises the following steps of: (1) preparing an aqueous perilla protein solution; (2) purifying the aqueous perilla protein solution; (3) concentrating the perilla protein solution; (4) preparing the nutrition powder of the perilla protein; and (5) recycling waste liquor. A traditional method for extracting the perilla protein is an alkali extraction and acid deposition method and comprises the following steps of: firstly dissolving the perilla protein from the perilla cake in an alkaline solution under a certain condition; and then, extracting a protein precipitation under an acid condition, and the method needs a great amount of alkali and acid, generates a great amount of acidic wastewater in the extraction process and has high energy consumption and lower yield and purity. In the invention, the extraction solution is purified by applying an ultrafiltration technology, the protein in the solution is extracted, the impurities of oligosaccharides, and the like is removed, and the oligosaccharides and the used extraction solution are recovered by applying a nanofiltration technology. The invention not only recycles reaction by-products, but also is pollution-free, water-saving, energy-saving and favorable to factory production.
Description
The present invention relates to a kind of method of doing purple perilla tropon of knowing, particularly extracted the method for purple perilla tropon by purple perilla cake,
Protein is the basis of life, is the important component part of body cell; It forms and repairing tissue, maintain the normal metabolism of human body and the normal immunologic function of human body, provide physical activity needed energy, there is no life without protein, protein is in the essential nutrient of humans and animals, to rank the first most important nutrient.Protein is divided into animal protein and vegetable protein according to its source, what have because phytoprotein fat content is low is not fatty even, the crowd that is particularly suitable for eating low fat food, as the elderly, needs the people of fat-reducing, particularly suffers from the people of high fat of blood, diabetes, fatty liver.In recent years to phytoprotein particularly protein content high, the demand of protein easily absorbed by the body increases year by year, exploitation quality plant albumen more and more comes into one's own.
Purple perilla is that first 60 kinds of Ministry of Public Health's announcement are the Plants in medicine and food, the industrial crops that have important exploitation to be worth in food and medicine field.Carrying in the purple perilla cake after oil protein content very abundant, is 33-35%, and wherein the content of lysine, methionine is all higher than soybean protein, almond and walnut protein.Be the albumen powder of raw material production with purple perilla grouts, have aroma, mouthfeel to be all better than existing plant protein powder; Because it is not containing urine agent enzyme, not containing toxic substances such as gossypol, sulphur glucoside, cyanogenic glycosides, edible safety is higher than the albumen powder extracting taking dregs of beans, cotton dregs, the dish dregs of rice, flax dregs of rice etc. as raw material.At present, perilla leaf, perilla herb oil are developed and applied, and the report of producing high nutrition albumen powder taking purple perilla cake as raw material is little, and existing technology is also the heavy extracting method of the molten acid of alkali, and the method exists pollution larger, the shortcoming that energy consumption is large.
The object of this invention is to provide a kind of method of being extracted purple perilla tropon by purple perilla cake, it can solve in prior art needs with a large amount of alkali and acid, not only water wasting but also can produce large amount of sewage, and energy consumption is high, does not meet the requirement of energy-saving and emission-reduction.Method technique provided by the invention is simple, and cost is low, and accessory substance can be recycled, pollution-free.Technical scheme of the present invention is as follows: a kind of method of being extracted purple perilla tropon by purple perilla cake, and its feature comprises the following steps:
A. prepare purple perilla protein solution
The purple perilla cake of carrying after oily degreasing was pulverized to 60 order Sieve, added alkaline water, and added thermal agitation and extract, filtered containing the purple perilla protein solution of compound sugar;
B. purifying purple perilla protein solution
Purple perilla protein solution is crossed under uniform temperature and pressure to milipore filter, removed compound sugar, obtain the purple perilla protein solution after purifying;
C. concentrated purple perilla protein solution
Purple perilla protein solution after purifying is concentrated, make protein content in solution reach 15-20%;
D. prepare purple perilla tropon
By dry the purple perilla protein solution spraying after concentrated, make purple perilla tropon
E. the recycling of waste liquid
The waste liquid that purifying purple perilla protein solution is produced is crossed NF membrane, and trapped fluid is the solution containing compound sugar, and it is concentrated, spraying is dried, and obtains compound sugar product; Penetrating fluid is the aqueous solution, can be recycled.
Described in steps A is sodium hydroxide solution for regulating the alkali of solution ph, and sodium hydroxide solution concentration is 20%, the PH=8 of extract;
Extraction temperature described in steps A is 50 degree, and extraction time is 55 minutes;
Solid-liquid ratio described in steps A is 1: 11;
The milipore filter material of the purifying purple perilla protein solution described in step B is PS membrane, and the molecular weight of holding back is 14000Da;
The milipore filter operating temperature of the purifying purple perilla protein solution described in step B is: 40 degree, and operating pressure is: 0.4Mpa;
In step C, be 60 degree for the temperature of reduced pressure concentration refined solution, gained feed liquid protein content is 15-20%
Spraying drying condition for the preparation of purple perilla tropon in step D is: inlet temperature 170 is spent, outlet temperature: 80 degree;
NF membrane for Recycling of waste liquid in step e is ceramic membrane, and the molecular mass of holding back is: 300Da, and operating pressure is 0.36MP, operating temperature is 30 degree;
The present invention adopts milipore filter and Nanofiltration-membrane technique to replace alkali in prior art to put forward the method for the plant separation protein matter of the high nutrition of the heavy preparation of acid.This method has been extracted purple perilla protein and the compound sugar aqueous solution with weak base aqueous solution, with milipore filter separation and purification purple perilla albumen, through concentrated, spraying dry the protein content purple perilla tropon that is greater than 90%.Through nanofiltration, concentrated, spraying dry extraction the compound sugar in waste liquid, the water circulation reclaiming is used.In sum, advantage of the present invention is: extraction cost is low, and water used can be recycled, pollution-free, energy-conservation; Due to the ultrafiltration of China in recent years and the production technology of nano-filtration membrane equipment very ripe, therefore technique of the present invention be extremely beneficial to batch production produce.
With embodiment, the present invention is illustrated below:
Embodiment: with purple perilla cake extraction purple perilla tropon, comprise the following steps:
A. prepare purple perilla protein solution: extract the aqueous solution containing purple perilla albumen and compound sugar;
Purple perilla cake after 2KG degreasing is pulverized, crossed 60 Mu Sieve, add in the reactor of 50L, add 22L deionized water, the sodium hydroxide solution that is 20% by concentration is adjusted PH=8, is heated with stirring to 50 degree, at this temperature, stirs and extracts 55 minutes; Use centrifuge centrifugal filtration, obtain purple perilla protein solution 22L.Filter residue can make ox feed.
B. purifying purple perilla protein solution: purple perilla albumen and compound sugar are separated, obtain purple perilla protein solution:
It is in the receiver of ultrafiltration apparatus of 14000Da that 22L purple perilla protein solution is added to film trapped molecular weight, the operating temperature of conditioning equipment is 40 degree, operating pressure is 0.4MPa, when liquid in receiver less than start time 1/5th, gradation adds the about 20L of deionized water in tank, until the PH that flows out water is lower than 7; Export trapped fluid with pump, add a small amount of deionized water and wash out ducted solution, obtain the about 10L of purifying purple perilla protein solution.
C. concentrated purple perilla protein solution: the operating temperature of vacuum concentration equipment is adjusted to 55 degree, the purple perilla protein solution after purifying is added in receiver, through concentrating to obtain concentrate 2.6L;
D. prepare purple perilla tropon: the inlet temperature of Frequency Conversion Technique of Centrifugal Spraying Drying Equipment is adjusted to 170 degree, and outlet temperature is adjusted to 80 degree, with peristaltic pump, solution is inputted to tower body, flow is as the criterion to protect stable outlet temperature seized with terror, and in this example, flow is 50ml/ minute; From collecting 616 grams of the purple perilla tropons that the protein content that makes is 91.3% at the bottom of tower.
E. the recycling of waste liquid: the operating pressure that is 300Da nano-filtration membrane equipment by film trapped molecular weight is set as 0.36Mpa, operating temperature is 30 degree, the liquid that sees through collected step B is crossed to nanofiltration, the solution of holding back is washed to neutrality by deionized water, be dried to obtain compound sugar crude product through concentrated, spraying; Nanofiltration sees through the extract of liquid for steps A.
Claims (2)
1. a method of being extracted purple perilla tropon by purple perilla cake, its feature comprises the following steps:
A. prepare purple perilla protein solution:
The purple perilla cake of carrying after oily degreasing was pulverized to 60 mesh sieves, add deionized water, solid-liquid ratio is 1: 11 (W/V), and the sodium hydroxide solution that is 20% by concentration regulates the pH=8 of the aqueous solution, add thermal agitation at 50 DEG C and extract 55 minutes, filter to obtain purple perilla protein solution;
B. purifying purple perilla protein solution
Purple perilla protein solution is crossed under 40 DEG C of temperature, 0.4Mpa pressure to milipore filter, the molecular cut off of film is 14000Da, and trapped fluid is the purple perilla protein solution after purifying;
C. concentrated purple perilla protein solution
By the reduced pressure concentration at 60 DEG C of temperature of the purple perilla protein solution after purifying, making protein content in solution is 15-20%;
D. purple perilla tropon preparation
By dry the purple perilla protein solution spraying after concentrated, spraying drying condition is: 170 DEG C of inlet temperatures, 80 DEG C of outlet temperatures: make purple perilla tropon;
E. the recycling of waste liquid
The waste liquid of purifying purple perilla protein solution generation is crossed to NF membrane under 30 DEG C of temperature and 0.36Mpa pressure, and the molecular cut off of film is 300Da, and trapped fluid, for containing oligosaccharide solution, concentrates it, spray and be dry, obtains compound sugar byproduct; Seeing through liquid is alkaline water, can be recycled.
2. the method for being extracted purple perilla tropon by purple perilla cake according to claim 1, it is characterized in that: in step e, using nanofiltration technology is recycled the waste liquid after purifying purple perilla albumen, the solution that NF membrane is held back adopts concentrated, the spraying drying condition identical with producing purple perilla tropon, makes compound sugar; The aqueous solution that NF membrane sees through returns in steps A as the extraction aqueous solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910147929.6A CN101919485B (en) | 2009-06-11 | 2009-06-11 | Method for extracting perilla protein nutrition powder from perilla cake |
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| CN200910147929.6A CN101919485B (en) | 2009-06-11 | 2009-06-11 | Method for extracting perilla protein nutrition powder from perilla cake |
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| CN101919485A CN101919485A (en) | 2010-12-22 |
| CN101919485B true CN101919485B (en) | 2014-09-03 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102696858A (en) * | 2012-05-25 | 2012-10-03 | 淄博十里香食品有限公司 | Preparation method for high-purity walnut protein powder |
| CN106615599A (en) * | 2016-09-14 | 2017-05-10 | 黑龙江省科学院大庆分院 | Purple perilla protein powder preparation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1336333A (en) * | 2001-09-25 | 2002-02-20 | 清华同方股份有限公司 | Membrane separation process of treating soybean processing waste water |
| CN1364763A (en) * | 2001-01-19 | 2002-08-21 | 北京中联捷思科技发展有限公司 | Method for extracting soy bean oligosaccharide from soy bean whey water |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1364763A (en) * | 2001-01-19 | 2002-08-21 | 北京中联捷思科技发展有限公司 | Method for extracting soy bean oligosaccharide from soy bean whey water |
| CN1336333A (en) * | 2001-09-25 | 2002-02-20 | 清华同方股份有限公司 | Membrane separation process of treating soybean processing waste water |
Non-Patent Citations (2)
| Title |
|---|
| 朱国君.紫苏饼粕蛋白质的分离提取及其功能特性研究.《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》.2008,(第10期),B018-16. * |
| 郭宏等.超滤技术在大豆分离蛋白生产中的应用研究.《食品科学》.2000,第21卷(第10期),56-58. * |
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