CN1364763A - Method for extracting soy bean oligosaccharide from soy bean whey water - Google Patents
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- CN1364763A CN1364763A CN 01101965 CN01101965A CN1364763A CN 1364763 A CN1364763 A CN 1364763A CN 01101965 CN01101965 CN 01101965 CN 01101965 A CN01101965 A CN 01101965A CN 1364763 A CN1364763 A CN 1364763A
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Abstract
The process of extracting soybean oligosaccharide from soybean whey water includes pre-treatment, ultrafiltration and nano filtering, the pre-treating is depositing, filtering and disinfecting whey water; the ultrafiltration is to eliminate whey protein from whey water; and the nano filtering is to use nano filtering membrane to desalt and concentrate so as to obtain soybean oligosaccharide liquid. The soybean oligosaccharide liquid may be further concentrated to obtain soybean oligosaccharide syrup, which may be spray dried to obtain soybean oligosaccharide powder. The said soybean oligosaccharide liquid and/or syrup may be decolored to obtain white soybean oligosaccharide liquid, syrup and powder. The process has no membrane pollution and environmental pollution and is simply and non-expensive.
Description
The present invention relates to a kind of low extracting method of getting sugar, specifically, relate to a kind of method of from soybean whey water, extracting soybean oligosaccharide.
Soybean whey water is to produce in the process to the soyabean processing protein isolate, can produce in producing technologies such as soybean protein concentrate, soybean protein isolate and production bean curd, also can be obtained by hot water lixiviate skimmed soy beans dregs of beans.In the past, soybean whey water was regarded as waste water always, normally directly discharged or handled with traditional biochemical process.In recent years, along with the understanding to the functional of whey-protein, oligose and health protection effect deepens continuously, people more and more pay attention to the development and use to soybean whey water.
The 0.8-2.5% that total solid in the soybean whey water is about floating clear water mainly is made up of soybean whey protein, salt and carbohydrate three class materials, and its composition situation sees Table 1.
The composition (W/V) of table 1 soybean whey water
| Soybean whey protein | Carbohydrate | Salt | Total solid |
| ????0.1-0.5％ | ???0.5-1％ | ???0.2-1％ | ????0.8-2.5％ |
The content of contained carbohydrate is about 0.5-1% in the soybean whey water, comprises disaccharide (sucrose), trisaccharide (raffinose) and tetrose (stachyose), and contents of monosaccharides is very little.Sucrose, stachyose and raffinose are referred to as soybean oligosaccharide.Wherein, stachyose and silk floss sugar have unique physiologically active, can breed the bifidus bacillus in the human intestinal in a large number, significantly improve the enteron aisle digestive function.Soybean oligosaccharide is very fast-selling product abroad.The development of China's soybean oligosaccharide is scarcely out of swaddling-clothes, and consumers demand increases very fast.
Soybean whey protein is the heavy albumen of non-acid, accounts for 0.1-0.5% in soybean whey water, is the 6-20% of total solid.Another part material in the soybean whey water is the salt of organic acid and mineral acid.Organic acid salt such as Citrate trianion, malate, succinate, acetate and Jiao's property bran propylhomoserin salt, inorganic acid salt are included in the leaching process because of adding sodium-chlor that acids or alkaloids form, calcium chloride etc.Usually at the soybean whey water of alkali extraction and acid precipitation technology gained, its organic acid and mineral acid salt total amount account for 0.2-1%.
Membrane technique extracts the soybean oligosaccharide in the soybean whey water, and research at home starts from the nineties later stage.The technology of now relevant with membrane technique separating oligomeric sugar generally is to adopt reverse osmosis concentration, then with resin or electrodialytic desalting.Complex process, operation stability is poor, and operation consumes big.The advantage of membrane technique is not fully used.And, adopting the resin desalination, resin needs frequent regeneration, consumes a large amount of chemical agents, the regenerated liquid contaminate environment, electrodialytic desalting, power consumption is big, and also has the handling problem of the strong brine that contains high-enriched organics.
The objective of the invention is to overcome above-mentioned technological difficulties and a kind of membrane technique advantage of giving full play to is provided, the method for from soybean whey water, extracting soybean oligosaccharide that film pollutes and environmental pollution is little.
Above-mentioned purpose of the present invention reaches by adopting membrane technique, and its concrete technical scheme is as follows.
The method of extracting soybean oligosaccharide from soybean whey water of the present invention comprises the steps:
Pre-treatment is that soybean whey water is precipitated, and filters and germicidal treatment, to remove insoluble suspended substance and the microorganism in the soybean whey water, guarantees the normal operation of subsequent ultrafiltration film;
Ultrafiltration, be to be the film of 10000-30000 by molecular weight cut-off with above-mentioned soybean whey water through pre-treatment, obtaining containing soluble solid concentration is 0.7-2.1%, wherein ash content is the liquid that sees through of 10-25%, the material of its described film is polyethersulfone, polysulfones or polyvinylidene difluoride (PVDF), and its form is rolled film, hollow-fibre membrane or tubular membrane; PH value in its operation process condition is 2-4, and cleans at least 1 time in moving 2 days.
Nanofiltration, be with above-mentioned ultrafiltration see through liquid by nanofiltration membrane, obtaining containing soluble solid concentration is 3-9%, wherein ash content is lower than 8% liquid glucose.
The method of from soybean whey water, extracting soybean oligosaccharide of the present invention, other operation process condition of wherein said ultrafiltration is: operating pressure 0.1-0.7MPa; Operating temperature 35-45 ℃; Circular flow makes film surface transverse flow speed reach 1-3m/s; The molecular weight cut-off of described nanofiltration membrane is 300-1000, and the material of its described film is polyethersulfone, polysulfones or polyvinylidene difluoride (PVDF), and its form is rolled film, hollow-fibre membrane or tubular membrane; The operation process condition is: operating pressure 1.0-1.8MPa; Operating temperature 35-45 ℃; Circular flow makes film surface transverse flow speed reach 1-3m/s; PH value 5-8, and in moving 3 days, clean at least 1 time.
The method of extracting soybean oligosaccharide from soybean whey water of the present invention also comprises with conventional concentrating under reduced pressure method described liquid glucose being concentrated into the syrupy step that its soluble solid concentration is 15-30%.
The method of extracting soybean oligosaccharide from soybean whey water of the present invention also comprises and uses conventional spray-drying process, and above-mentioned syrup is dried to the step that moisture is lower than 3% soybean oligosaccharide powder.
The method of extracting soybean oligosaccharide from soybean whey water of the present invention also comprises described liquid glucose or/and the step that syrup decolours.
The present invention extracts the method for soybean oligosaccharide from soybean whey water, wherein said decolouring is to adopt the powder activity carbon decoloring.
The beneficial effect that extracts the method for soybean oligosaccharide from soybean whey water of the present invention is:
1, effectively solves membrane pollution problem
Because the high viscosity of soybean protein makes it very easily to be adsorbed on the film, causes the rapid reduction of membrane flux.And that film cleans is not thorough, often makes film device be tending towards paralysis very soon.Present method has been selected suitable membrane, has adopted suitable operational conditions, as correct operating pressure, temperature, pH value and purging method etc., can solve the film pollution problems effectively, guarantees the steady running of film device, and then has guaranteed the long-term stability operation of production.
2, technology is simple and direct, and expense is cheap
Prior art adopts reverse osmosis concentration, then with resin or electrodialytic desalting, and its complex process, operation stability is poor, and operation energy consumption is big, and adopts the resin desalination, and resin needs frequent regeneration, consumes a large amount of chemical agents, and regenerated liquid pollutes the environment.Adopt electrodialytic desalting, power consumption is big, also has the handling problem of the strong brine that contains high-enriched organics.Present method ultra-filtration and separation albumen concentrates and desalination with nanofiltration, can reduce environmental pollution, and simplify technology, has reduced working cost.
3, constant product quality, quality improves a lot
The raising of product quality on the one hand is that present method goes out oligose and albumen with membrane separating, the local flavor of product is maintained and improves; On the other hand, utilize oligose that present method produces, and their content reach more than 40%, greater than the standard of similar products at home and abroad based on stachyose and raffinose.
Embodiment
To extract the soybean oligosaccharide powder with method of the present invention from soybean whey water is that example is described in detail as follows.
Certain soybean protein isolate manufacturer, the soybean whey water that is produced is 340 tons/day.At first with board-like Sterilizers soybean whey water is carried out sterilising treatment, sterilising temp is 100 ℃, and sterilization time was 3 seconds; Again through 3 hours precipitations; Filter with deep bed filter then; Enter ultrafiltration apparatus subsequently, wherein using molecular weight cut-off is 10000 polyethersulfone rolling ultra-filtration membrane, the operation process condition is: pressure changes between 0.15-0.3MPa, 38 ℃ of temperature, circular flow makes film surface transverse flow speed reach 2m/s, pH value 3.0, solid concentration be 1.39% see through 306 tons of liquid; Make this see through liquid and enter the desalination of nanofiltration equipment and concentrate, wherein using molecular weight cut-off is 300 polyethersulfone rolled film, and the operation process condition is: pressure 1.5MPa, 40 ℃ of temperature.Circular flow makes film surface transverse flow speed reach 2m/s, and pH value 6.0 obtains solid concentration and be 38 tons of 6.36% concentrated soybean oligosaccharide liquid; This concentrated soybean oligosaccharide liquid is carried out the powder activity carbon decoloring, make its color and luster pure white, wherein the Powdered Activated Carbon consumption is 1% of this liquid glucose weight, and bleaching temperature is 80 ℃, and bleaching time is 30 minutes; Concentrate the liquid glucose of described decolouring then with the economic benefits and social benefits concentration evaporator, obtain concentration and reach 20% soybean oligosaccharide slurry, wherein thickening temperature is 88 ℃, and vacuum tightness is-0.06MPa; At last described syrup is carried out spraying drying, obtain stachyose and raffinose content and be 1.2 tons/day in 44% soybean oligosaccharide powder, its moisture is lower than 3%.The inlet temperature of used spray-drying tower is 170 ℃, and air outlet temperature is 80 ℃, and tower is pressed and is-100Pa.In continuous leaching process, the every operation of ultrafiltration and nanofiltration 20 hours will be cleaned respectively 1 time ultra-filtration membrane and nanofiltration membrane, comprise earlier with 45 ℃ hot water injection 15 minutes, clean twice with the NaOH solution chemistry of PH=11.5 again, every all over each 45 minutes, so that recover the membrane flux of described film fully.
Use method of the present invention can obtain respectively without soybean oligosaccharide liquid, syrup and the Icing Sugar of decolouring and pure white liquid glucose, syrup and the Icing Sugar of back color and luster that decolour, for different uses.
Claims (8)
1, a kind of method of extracting soybean oligosaccharide from soybean whey water, it comprises the steps:
Pre-treatment is that soybean whey water is precipitated, and filters and germicidal treatment, to remove insoluble suspended substance and the microorganism in the soybean whey water, guarantees the normal operation of subsequent ultrafiltration film;
Ultrafiltration, be to be the film of 10000-30000 by molecular weight cut-off with above-mentioned soybean whey water through pre-treatment, obtaining containing soluble solid concentration is 0.7-2.1%, wherein ash content is the liquid that sees through of 10-25%, the material of its described film is polyethersulfone, polysulfones or polyvinylidene difluoride (PVDF), and its form is rolled film, hollow-fibre membrane or tubular membrane; PH value in its operation process condition is 2-4, and cleans at least 1 time in moving 2 days.
Nanofiltration, be with above-mentioned ultrafiltration see through liquid by nanofiltration membrane, obtaining containing soluble solid concentration is 3-9%, wherein ash content is lower than 8% liquid glucose.
2, the method for from soybean whey water, extracting soybean oligosaccharide according to claim 1, other operation process condition of wherein said ultrafiltration is: operating pressure 0.1-0.7MPa; Operating temperature 35-45 ℃; Circular flow makes film surface transverse flow speed reach 1-3m/s; The molecular weight cut-off of described nanofiltration membrane is 300-1000, and the material of its described film is polyethersulfone, polysulfones or polyvinylidene difluoride (PVDF), and its form is rolled film, hollow-fibre membrane or tubular membrane; The operation process condition is: operating pressure 1.0-1.8MPa; Operating temperature 35-45 ℃; Circular flow makes film surface transverse flow speed reach 1-3m/s; PH value 5-8, and in moving 3 days, clean at least 1 time.
3, the method for extracting soybean oligosaccharide from soybean whey water according to claim 1 and 2 also comprises with conventional concentrating under reduced pressure method described liquid glucose being concentrated into the syrupy step that its soluble solid concentration is 15-30%.
4, the method for extracting soybean oligosaccharide from soybean whey water according to claim 3 also comprises and uses conventional spray-drying process, and above-mentioned syrup is dried to the step that moisture is lower than 3% soybean oligosaccharide powder.
5, the method for extracting soybean oligosaccharide from soybean whey water according to claim 1 and 2 also comprises described liquid glucose or/and the step that syrup decolours.
6, the method for extracting soybean oligosaccharide from soybean whey water according to claim 3 also comprises described liquid glucose or/and the step that syrup decolours.
7, the method for extracting soybean oligosaccharide from soybean whey water according to claim 5, described decolouring is to adopt the powder activity carbon decoloring.
8, the method for extracting soybean oligosaccharide from soybean whey water according to claim 6, described decolouring is to adopt the powder activity carbon decoloring.
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| CN 01101965 CN1128151C (en) | 2001-01-19 | 2001-01-19 | Method for extracting soy bean oligosaccharide from soy bean whey water |
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| CN 01101965 CN1128151C (en) | 2001-01-19 | 2001-01-19 | Method for extracting soy bean oligosaccharide from soy bean whey water |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294144C (en) * | 2002-07-31 | 2007-01-10 | 不二制油株式会社 | Process for producing soy-bean whey separator |
| CN1903052B (en) * | 2005-07-26 | 2010-04-28 | 天津科技大学 | Method for prepairng whey powder containing casein phosphopeptide, anti-angiotonin converzyme peptide and oligo-galactose |
| CN101712715A (en) * | 2009-12-18 | 2010-05-26 | 周鑫 | Method for decolorizing full water-soluble soybean glycoprotein |
| CN102702274A (en) * | 2012-06-12 | 2012-10-03 | 华东理工大学 | Method for preparing high-purity soybean oligosaccharide from soybean whey wastewater |
| CN101961055B (en) * | 2009-07-22 | 2012-11-21 | 内蒙古伊利实业集团股份有限公司 | Desalting method of whey powder |
| CN103554192A (en) * | 2013-11-11 | 2014-02-05 | 齐鲁工业大学 | Method for extracting oligosaccharide from plant material prehydrolysis liquid |
| CN103896994A (en) * | 2014-03-11 | 2014-07-02 | 黑龙江八一农垦大学 | Method for separating and extracting oligosaccharide from soybean whey waste liquid |
| CN101919485B (en) * | 2009-06-11 | 2014-09-03 | 丰宁京北第一草原原生态食品科技开发有限公司 | Method for extracting perilla protein nutrition powder from perilla cake |
| CN104530143A (en) * | 2014-12-31 | 2015-04-22 | 东北农业大学 | Method for preparing soybean whey oligosaccharide through coupling of heating, flocculating, air floating and ultra-filtering |
| CN105410328A (en) * | 2015-12-16 | 2016-03-23 | 新希望双喜乳业（苏州）有限公司 | Method for extracting whey protein powder from milk |
| CN106518906A (en) * | 2016-09-24 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane-method extraction process for soybean lecithin |
| CN109851133A (en) * | 2019-03-29 | 2019-06-07 | 中国科学院过程工程研究所 | A method of preventing starch hydrolyzate desalination processes microbial contamination |
| CN111139318A (en) * | 2019-12-11 | 2020-05-12 | 黑龙江省农垦龙王食品有限公司 | Method for extracting soybean oligosaccharide |
-
2001
- 2001-01-19 CN CN 01101965 patent/CN1128151C/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294144C (en) * | 2002-07-31 | 2007-01-10 | 不二制油株式会社 | Process for producing soy-bean whey separator |
| CN1903052B (en) * | 2005-07-26 | 2010-04-28 | 天津科技大学 | Method for prepairng whey powder containing casein phosphopeptide, anti-angiotonin converzyme peptide and oligo-galactose |
| CN101919485B (en) * | 2009-06-11 | 2014-09-03 | 丰宁京北第一草原原生态食品科技开发有限公司 | Method for extracting perilla protein nutrition powder from perilla cake |
| CN101961055B (en) * | 2009-07-22 | 2012-11-21 | 内蒙古伊利实业集团股份有限公司 | Desalting method of whey powder |
| CN101712715A (en) * | 2009-12-18 | 2010-05-26 | 周鑫 | Method for decolorizing full water-soluble soybean glycoprotein |
| CN102702274B (en) * | 2012-06-12 | 2015-01-28 | 华东理工大学 | Method for preparing high-purity soybean oligosaccharide from soybean whey wastewater |
| CN102702274A (en) * | 2012-06-12 | 2012-10-03 | 华东理工大学 | Method for preparing high-purity soybean oligosaccharide from soybean whey wastewater |
| CN103554192A (en) * | 2013-11-11 | 2014-02-05 | 齐鲁工业大学 | Method for extracting oligosaccharide from plant material prehydrolysis liquid |
| CN103554192B (en) * | 2013-11-11 | 2016-01-20 | 齐鲁工业大学 | A kind of method extracting oligose from plant material pre-hydrolyzed solution |
| CN103896994A (en) * | 2014-03-11 | 2014-07-02 | 黑龙江八一农垦大学 | Method for separating and extracting oligosaccharide from soybean whey waste liquid |
| CN103896994B (en) * | 2014-03-11 | 2017-01-18 | 黑龙江八一农垦大学 | Method for separating and extracting oligosaccharide from soybean whey waste liquid |
| CN104530143A (en) * | 2014-12-31 | 2015-04-22 | 东北农业大学 | Method for preparing soybean whey oligosaccharide through coupling of heating, flocculating, air floating and ultra-filtering |
| CN105410328A (en) * | 2015-12-16 | 2016-03-23 | 新希望双喜乳业（苏州）有限公司 | Method for extracting whey protein powder from milk |
| CN106518906A (en) * | 2016-09-24 | 2017-03-22 | 合肥信达膜科技有限公司 | Membrane-method extraction process for soybean lecithin |
| CN109851133A (en) * | 2019-03-29 | 2019-06-07 | 中国科学院过程工程研究所 | A method of preventing starch hydrolyzate desalination processes microbial contamination |
| CN111139318A (en) * | 2019-12-11 | 2020-05-12 | 黑龙江省农垦龙王食品有限公司 | Method for extracting soybean oligosaccharide |
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| CN1128151C (en) | 2003-11-19 |
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