CN101896272B - 蛋白酶检测 - Google Patents

蛋白酶检测 Download PDF

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Publication number
CN101896272B
CN101896272B CN2008801158369A CN200880115836A CN101896272B CN 101896272 B CN101896272 B CN 101896272B CN 2008801158369 A CN2008801158369 A CN 2008801158369A CN 200880115836 A CN200880115836 A CN 200880115836A CN 101896272 B CN101896272 B CN 101896272B
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CN
China
Prior art keywords
amino acid
polypeptide
color development
product
protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2008801158369A
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English (en)
Chinese (zh)
Other versions
CN101896272A (zh
Inventor
詹姆斯·亚历山大·斯库藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mologic Ltd
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Mologic Ltd
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Publication date
Application filed by Mologic Ltd filed Critical Mologic Ltd
Publication of CN101896272A publication Critical patent/CN101896272A/zh
Application granted granted Critical
Publication of CN101896272B publication Critical patent/CN101896272B/zh
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B01L3/5055—Hinged, e.g. opposable surfaces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0808—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms, e.g. Val, Ile, Leu
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2008801158369A 2007-11-13 2008-11-13 蛋白酶检测 Expired - Fee Related CN101896272B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0722287A GB2454672A (en) 2007-11-13 2007-11-13 Chromogenic protease substrates
GB0722287.0 2007-11-13
PCT/GB2008/003833 WO2009063208A2 (en) 2007-11-13 2008-11-13 Protease detection

Publications (2)

Publication Number Publication Date
CN101896272A CN101896272A (zh) 2010-11-24
CN101896272B true CN101896272B (zh) 2013-07-31

Family

ID=38896238

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008801158369A Expired - Fee Related CN101896272B (zh) 2007-11-13 2008-11-13 蛋白酶检测

Country Status (11)

Country Link
US (2) US8993253B2 (enExample)
EP (1) EP2222404B1 (enExample)
JP (1) JP5667877B2 (enExample)
CN (1) CN101896272B (enExample)
AU (1) AU2008322724B2 (enExample)
CA (1) CA2705432A1 (enExample)
GB (1) GB2454672A (enExample)
IL (1) IL205699A0 (enExample)
NZ (1) NZ585207A (enExample)
WO (1) WO2009063208A2 (enExample)
ZA (1) ZA201003313B (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2435510A (en) 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
HRP20170929T1 (hr) 2008-09-17 2017-09-22 Chiasma Inc. Farmaceutski pripravci i odgovarajući postupci za isporuku
GB201116523D0 (en) 2011-09-23 2011-11-09 Systagenix Wound Man Ip Co Bv Wound prognosis
EP2896963B1 (en) 2011-01-31 2019-01-30 Woundchek Laboratories B.V. Wound prognosis
EP2734846A4 (en) * 2011-07-22 2015-03-18 Rapid Pathogen Screening Inc LATERAL FLOW ASSAYS BASED ON ENZYMATIC CLIP
GB201206976D0 (en) 2012-04-20 2012-06-06 Mologic Ltd An enzyme detection device
GB201206977D0 (en) * 2012-04-20 2012-06-06 Mologic Ltd An enzyme detection device
JP6510747B2 (ja) * 2013-08-02 2019-05-08 東洋ビーネット株式会社 プロテアーゼ活性測定法
AU2016215350B2 (en) 2015-02-03 2021-11-25 Amryt Endo, Inc. Method of treating diseases
GB201605110D0 (en) 2016-03-24 2016-05-11 Mologic Ltd Detecting sepsis
GB201614053D0 (en) * 2016-08-17 2016-09-28 Microarray Ltd Determining the condition of a wound
GB201902458D0 (en) 2019-02-22 2019-04-10 Mologic Ltd Treatment stratification for an excerbation of inflamation
US11141457B1 (en) 2020-12-28 2021-10-12 Amryt Endo, Inc. Oral octreotide therapy and contraceptive methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007096637A1 (en) * 2006-02-23 2007-08-30 Mologic Ltd Protease detection
WO2007096642A1 (en) * 2006-02-23 2007-08-30 Mologic Ltd Enzyme detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5988099A (ja) * 1982-11-15 1984-05-21 Toyo Jozo Co Ltd 新規な酵素活性の測定法
US4588836A (en) * 1982-09-01 1986-05-13 Toyo Jozo Kabushiki Kaisha Novel synthetic substrate and assay method using the same
JPS5942350A (ja) * 1982-09-01 1984-03-08 Toyo Jozo Co Ltd 新規な合成基質を用いるＬ‐ロイシンアミノペプチダーゼおよびγ‐グルタミルトランスペプチダーゼからなる群より選ばれるペプチダーゼの活性測定法
DE3413311A1 (de) * 1984-04-09 1985-10-17 Behringwerke Ag, 3550 Marburg Reagenz zur bestimmung der thromboplastinzeit
HU194913B (en) * 1986-01-03 1988-03-28 Innofinance Altalanos Innovaci Process for producing novel gonadoliberin derivatives containing in the sixth position aromatic amino carboxylic acid and medical preparations containing these compounds

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007096637A1 (en) * 2006-02-23 2007-08-30 Mologic Ltd Protease detection
WO2007096642A1 (en) * 2006-02-23 2007-08-30 Mologic Ltd Enzyme detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Synthesis of peptide p-nitroanilides mimicking fibrinogen- and hirudin-binding to thrombin;Dirk T.S. Rijkers et al.;《International journal of Peptide ﹠ Protein Research》;19960331;第48卷;182-193 *

Also Published As

Publication number Publication date
CN101896272A (zh) 2010-11-24
ZA201003313B (en) 2011-08-31
AU2008322724A1 (en) 2009-05-22
NZ585207A (en) 2011-09-30
US8993253B2 (en) 2015-03-31
IL205699A0 (en) 2010-11-30
WO2009063208A3 (en) 2009-08-13
EP2222404A2 (en) 2010-09-01
JP2011503160A (ja) 2011-01-27
WO2009063208A2 (en) 2009-05-22
GB2454672A (en) 2009-05-20
EP2222404B1 (en) 2018-08-01
US9376706B2 (en) 2016-06-28
AU2008322724B2 (en) 2014-03-27
GB0722287D0 (en) 2007-12-27
JP5667877B2 (ja) 2015-02-12
CA2705432A1 (en) 2009-05-22
US20150152471A1 (en) 2015-06-04
US20110086370A1 (en) 2011-04-14

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