CN101893624A - Westernblot reaction box - Google Patents
Westernblot reaction box Download PDFInfo
- Publication number
- CN101893624A CN101893624A CN2010102048558A CN201010204855A CN101893624A CN 101893624 A CN101893624 A CN 101893624A CN 2010102048558 A CN2010102048558 A CN 2010102048558A CN 201010204855 A CN201010204855 A CN 201010204855A CN 101893624 A CN101893624 A CN 101893624A
- Authority
- CN
- China
- Prior art keywords
- box
- reaction
- tubes
- lid
- reaction box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a westernblot reaction box. The reaction box comprises a box body and a box cover, wherein silicon gel seal strips are arranged in the box cover and used for guaranteeing that the cover is sealed on the box body; vent holes are formed on the box cover and linked with micro silicone tubes; a cap is arranged at the tail end of each micro silicone tube; and the vent holes are formed on the end sides of the box cover. Usage comprises the following steps of: implanting a carrier film in the reaction box, adding a reaction reagent by using a dropper, standing to make bubbles rise, filling reagent solution up and covering; when covering, opening the micro silicone tubes to make air or redundant liquid overflow from the tubes, then covering, sealing the micro silicon tubes and performing reaction in a sealing state; and keeping the reaction box in a sealed state without liquid leakage after closing the micro silicon tubes. The reaction box of the invention has the advantages of simple structure, low manufacturing cost, simple operation, a few required reagent, and wide application in labs of all levels.
Description
Technical field
The present invention relates to a kind of westernblot reaction box.
Background technology
After the westernblot test is meant polyacrylamide gel electrophoresis, the protein band in the gel being transferred on the nitrocellulose filter, is the testing process that carrier carries out immunological response and colour developing then with the nitrocellulose filter.The concrete operations step is as follows: 1. sealing: after the polyacrylamide gel electrophoresis band is transferred to the cellulose nitrate membrane carrier, carrier film is placed confining liquid, seal not protein-bonded as yet site; 2. antigen-antibody reaction: albumen and specific antibody on the carrier film carrier are reacted; 3. mark: allow be incorporated on the carrier film specific antibody again with two anti-reactions of marker enzyme; 4. colour developing: brown particle takes place in the DAB in the colour developing damping fluid under the enzymatic on the carrier film, and is deposited in the brown band of appearance on the carrier film.
At present, the course of reaction of membrane carrier is mainly carried out in plate or in the sealed plastic bag, and plate uses amount of reagent big, and the sealed plastic bag trivial operations, because antibody reagent costs an arm and a leg, though though the sealed plastic bag complicated operation currently still generally adopt for most of laboratories.
Summary of the invention
At above-mentioned prior art, the invention provides a kind of simple to operate, few westernblot reaction box of use amount of reagent.
The present invention is achieved by the following technical solutions:
A kind of westernblot reaction box comprises box body, lid, wherein, is provided with the silica gel strip of paper used for sealing in the lid, sealing when covering on box body with assurance, and lid is provided with air hole, the little silicone tube of link on the air hole, little silicone tube end is provided with block.
Described box body is rectangular build, and the box space height is 1mm.
It is distolateral that described air hole is located at lid.
The size of described box body can be selected for use according to the size of carrier film.
Described box body, lid are colourless transparent material and make, observations such as convenient removal bubble, colour developing.
Described box body, lid select for use suicide material to make, in order to avoid albumen or nucleic acid in the vessel surface absorption reagent.
Use: at first carrier film is implanted reaction box of the present invention, added reaction reagent with dropper, the static bubble that makes rises, and fills it up with reagent solution, covers lid.During the hamper lid, open little silicone tube, air or unnecessary liquid are overflowed by pipeline, cover lid then, seal up little silicone tube, react under the sealing state.Reaction box is in sealing state after closing little silicone tube, can not the leakage body.
Reaction box of the present invention is simple in structure, and cost of manufacture is lower, and simple to operate, and required reagent is few, is fit to the laboratory applications of various levels.Reaction box of the present invention is not only applicable to westernblot, and is applicable to that RNA, DAN etc. are the reaction of carrier with the film, are widely used.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Fig. 2 is the structural representation of lid reverse side.
Fig. 3 is a longitudinal sectional view of the present invention.
Wherein, 1, box body; 2, box body neck; 3, lid; 4, silica gel strip of paper used for sealing; 5, air hole; 6, little silicone tube; 7, block; 8, reaction chamber.
Embodiment
The present invention is further illustrated below in conjunction with accompanying drawing:
A kind of westernblot reaction box comprises box body 1, lid 2, and wherein, box body 1 is rectangular build, and reaction chamber 8 (being the space of box body 1) is the flat cubic, highly is 1mm.Box body neck 2 is skinny, and the transversal section is smooth, and the width of lid 3 is enclosing casing neck 2 just in time, and the inner bottom part place mat of lid 3 has silica gel strip of paper used for sealing 4, and lid 3 enclosing casing necks 2 insert, and the flat disconnected of opening of box body 1 just in time docked with the silica gel strip of paper used for sealing 4 at the lid end.Lid 3 is provided with air hole 5, the little silicone tube 6 of link on the air hole 5, and little silicone tube 6 ends are provided with block 7.
It is distolateral that described air hole 5 is located at lid 3, and silica gel strip of paper used for sealing 4 can not seal it.
The size of described box body 1 can be selected for use according to the size of carrier film.
Described box body 1, lid 3 are colourless transparent material and make, observations such as convenient removal bubble, colour developing.
Described box body 1, lid 3 select for use suicide material to make, in order to avoid albumen or nucleic acid in the vessel surface absorption reagent.
Use: at first carrier film is implanted reaction chamber 8, added reaction reagent with dropper, the static bubble that makes rises, and fills it up with reagent solution, covers lid 3.Hamper covered 3 o'clock, opened little silicone tube 6, and air or unnecessary liquid are overflowed by pipeline, covered lid 3 then, sealed up little silicone tube 6, reacted under the sealing state.Reaction box is in sealing state after closing little silicone tube 6, can not the leakage body.
Claims (3)
1. westernblot reaction box, it is characterized in that: comprise box body, lid, wherein, be provided with the silica gel strip of paper used for sealing in the lid, lid is provided with air hole, the little silicone tube of link on the air hole, little silicone tube end is provided with block.
2. a kind of westernblot reaction box according to claim 1, it is characterized in that: described box body is rectangular build, and the box space height is 1mm.
3. a kind of westernblot reaction box according to claim 1, it is characterized in that: it is distolateral that described air hole is located at lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102048558A CN101893624A (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102048558A CN101893624A (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101893624A true CN101893624A (en) | 2010-11-24 |
Family
ID=43102883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102048558A Pending CN101893624A (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101893624A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2135154Y (en) * | 1992-09-03 | 1993-06-02 | 祝加贝 | Flat-piece testing drug box |
| CN2236658Y (en) * | 1995-06-14 | 1996-10-02 | 无锡市第一人民医院 | Tuberculous antibody diagnostic box by enzyme dotting process |
| CN2288443Y (en) * | 1997-03-25 | 1998-08-19 | 山东远东国际贸易公司 | External diagnosis reagent box for hepatitis |
| CN201724939U (en) * | 2010-06-22 | 2011-01-26 | 张子毅 | Westernblot reaction box |
-
2010
- 2010-06-22 CN CN2010102048558A patent/CN101893624A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2135154Y (en) * | 1992-09-03 | 1993-06-02 | 祝加贝 | Flat-piece testing drug box |
| CN2236658Y (en) * | 1995-06-14 | 1996-10-02 | 无锡市第一人民医院 | Tuberculous antibody diagnostic box by enzyme dotting process |
| CN2288443Y (en) * | 1997-03-25 | 1998-08-19 | 山东远东国际贸易公司 | External diagnosis reagent box for hepatitis |
| CN201724939U (en) * | 2010-06-22 | 2011-01-26 | 张子毅 | Westernblot reaction box |
Non-Patent Citations (1)
| Title |
|---|
| GENHUNTER公司: "PerfectWestern Container", 《FUNAKOSHI新闻》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110161266B (en) | Method for collecting liquid sample | |
| CN104668005B (en) | A kind of domestic micro-fluidic chip and using method thereof | |
| ATE356354T1 (en) | ANALYTICAL TEST SETUP FOR MEMBRANE BASED TESTS | |
| WO2010080115A2 (en) | Improved reagent storage in microfluidic systems and related articles and methods | |
| ATE1075T1 (en) | SHUT-OFF DEVICE FOR THE OUTLET OF A MEASURING CONTAINER, ESPECIALLY URINE MEASURING CONTAINER. | |
| CN112718031B (en) | Biochemical test paper tube | |
| CN104080536A (en) | Microchip under vacuum | |
| AU7557187A (en) | Improved apparatus and process for immunoassays | |
| JP2008292197A (en) | Device for test strip | |
| CN115011454A (en) | Nucleic acid detection device and method | |
| CN201724939U (en) | Westernblot reaction box | |
| CN207268737U (en) | A kind of drug abuse saliva detection cup | |
| CN101893624A (en) | Westernblot reaction box | |
| CN212722908U (en) | Biochemical test paper and test tube | |
| CN202305534U (en) | Western blotting antibody incubation box | |
| CN217733115U (en) | Nucleic acid detection device | |
| WO2021254513A1 (en) | Biochemical test paper tube | |
| CN103157524A (en) | Sample chamber with partition board | |
| CN101482558A (en) | Multi-reagent encapsulation of cuvette | |
| CN104841501A (en) | Micro-porous plate for packaging micro liquid | |
| CN204807462U (en) | Saliva sample chemical examination device | |
| CN206731144U (en) | A kind of reaction vessels for soil surface photodissociation test | |
| CN103642674A (en) | In situ polymerase chain reaction plate | |
| CN207904240U (en) | A kind of excretion body nucleic acid extraction kit | |
| CN204666632U (en) | Immunohistochemical staining device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101124 |
|
| WD01 | Invention patent application deemed withdrawn after publication |