CN201724939U - Westernblot reaction box - Google Patents
Westernblot reaction box Download PDFInfo
- Publication number
- CN201724939U CN201724939U CN2010202316985U CN201020231698U CN201724939U CN 201724939 U CN201724939 U CN 201724939U CN 2010202316985 U CN2010202316985 U CN 2010202316985U CN 201020231698 U CN201020231698 U CN 201020231698U CN 201724939 U CN201724939 U CN 201724939U
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- CN
- China
- Prior art keywords
- box
- silicone tube
- reaction
- box cover
- lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Abstract
The utility model discloses a westernblot reaction box, which comprises a box body and a box cover, wherein, a silica gel seal is arranged in the box cover to ensure that the box body is sealed when covered with the box cover; a venthole is formed on the box cover; a cost silicone tube is connected to the venthole; a nut cap is arranged at the tail end of the micro silicone tube; and the venthole is formed on one end side of the box cover. When the box is used, firstly, a carrier film is implanted in the reaction box; reaction reagent is filled into the box through a burette and enabled to be still, and causes bubbles to rise; and then box is full of the reagent solution and covered by the box cover. When the box cover is to be put on, the micro silicone tube is opened to enable air or excess liquid to overflow from a pipeline; then the box cover is put on, and the micro silicone tube is sealed; and the reagent reacts in the seal state. When the micro silicone tube is sealed, the reaction box is in the seal state and does not leak. The reaction box of the utility model has the advantages of simple structure, lower production cost, simple operation and less required reagent, and is applicable to laboratories of various levels.
Description
Technical field
The utility model relates to a kind of westernblot reaction box.
Background technology
After the westernblot test is meant polyacrylamide gel electrophoresis, the protein band in the gel being transferred on the nitrocellulose filter, is the testing process that carrier carries out immunological response and colour developing then with the nitrocellulose filter.The concrete operations step is as follows: 1. sealing: after the polyacrylamide gel electrophoresis band is transferred to the cellulose nitrate membrane carrier, carrier film is placed confining liquid, seal not protein-bonded as yet site; 2. antigen-antibody reaction: albumen and specific antibody on the carrier film carrier are reacted; 3. mark: allow be incorporated on the carrier film specific antibody again with two anti-reactions of marker enzyme; 4. colour developing: brown particle takes place in the DAB in the colour developing damping fluid under the enzymatic on the carrier film, and is deposited in the brown band of appearance on the carrier film.
At present, the course of reaction of membrane carrier is mainly carried out in plate or in the sealed plastic bag, and plate uses amount of reagent big, and the sealed plastic bag trivial operations, because antibody reagent costs an arm and a leg, though though the sealed plastic bag complicated operation currently still generally adopt for most of laboratories.
Summary of the invention
At above-mentioned prior art, the utility model provides a kind of simple to operate, few westernblot reaction box of use amount of reagent.
The utility model is achieved through the following technical solutions:
A kind of westernblot reaction box comprises box body, lid, wherein, is provided with the silica gel strip of paper used for sealing in the lid, sealing when covering on box body with assurance, and lid is provided with air hole, the little silicone tube of link on the air hole, little silicone tube end is provided with block.
Described box body is rectangular build, and the box space height is 1mm.
It is distolateral that described air hole is located at lid.
The size of described box body can be selected for use according to the size of carrier film.
Described box body, lid are colourless transparent material and make, observations such as convenient removal bubble, colour developing.
Described box body, lid select for use suicide material to make, in order to avoid albumen or nucleic acid in the vessel surface absorption reagent.
Use: at first carrier film is implanted reaction box of the present utility model, added reaction reagent with dropper, the static bubble that makes rises, and fills it up with reagent solution, covers lid.During the hamper lid, open little silicone tube, air or unnecessary liquid are overflowed by pipeline, cover lid then, seal up little silicone tube, react under the sealing state.Reaction box is in sealing state after closing little silicone tube, can not the leakage body.
Reaction box of the present utility model is simple in structure, and cost of manufacture is lower, and simple to operate, and required reagent is few, is fit to the laboratory applications of various levels.Reaction box of the present utility model is not only applicable to westernblot, and is applicable to that RNA, DAN etc. are the reaction of carrier with the film, are widely used.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is the structural representation of lid reverse side.
Fig. 3 is a longitudinal sectional view of the present utility model.
Wherein, 1, box body; 2, box body neck; 3, lid; 4, silica gel strip of paper used for sealing; 5, air hole; 6, little silicone tube; 7, block; 8, reaction chamber.
Embodiment
Below in conjunction with accompanying drawing the utility model is further described:
A kind of westernblot reaction box comprises box body 1, lid 2, and wherein, box body 1 is rectangular build, and reaction chamber 8 (being the space of box body 1) is the flat cubic, highly is 1mm.Box body neck 2 is skinny, and the transversal section is smooth, and the width of lid 3 is enclosing casing neck 2 just in time, and the inner bottom part place mat of lid 3 has silica gel strip of paper used for sealing 4, and lid 3 enclosing casing necks 2 insert, and the flat disconnected of opening of box body 1 just in time docked with the silica gel strip of paper used for sealing 4 at the lid end.Lid 3 is provided with air hole 5, the little silicone tube 6 of link on the air hole 5, and little silicone tube 6 ends are provided with block 7.
It is distolateral that described air hole 5 is located at lid 3, and silica gel strip of paper used for sealing 4 can not seal it.
The size of described box body 1 can be selected for use according to the size of carrier film.
Described box body 1, lid 3 are colourless transparent material and make, observations such as convenient removal bubble, colour developing.
Described box body 1, lid 3 select for use suicide material to make, in order to avoid albumen or nucleic acid in the vessel surface absorption reagent.
Use: at first carrier film is implanted reaction chamber 8, added reaction reagent with dropper, the static bubble that makes rises, and fills it up with reagent solution, covers lid 3.Hamper covered 3 o'clock, opened little silicone tube 6, and air or unnecessary liquid are overflowed by pipeline, covered lid 3 then, sealed up little silicone tube 6, reacted under the sealing state.Reaction box is in sealing state after closing little silicone tube 6, can not the leakage body.
Claims (3)
1. westernblot reaction box, it is characterized in that: comprise box body, lid, wherein, be provided with the silica gel strip of paper used for sealing in the lid, lid is provided with air hole, the little silicone tube of link on the air hole, little silicone tube end is provided with block.
2. a kind of westernblot reaction box according to claim 1, it is characterized in that: described box body is rectangular build, and the box space height is 1mm.
3. a kind of westernblot reaction box according to claim 1, it is characterized in that: it is distolateral that described air hole is located at lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202316985U CN201724939U (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202316985U CN201724939U (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201724939U true CN201724939U (en) | 2011-01-26 |
Family
ID=43493278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010202316985U Expired - Lifetime CN201724939U (en) | 2010-06-22 | 2010-06-22 | Westernblot reaction box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201724939U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893624A (en) * | 2010-06-22 | 2010-11-24 | 张子毅 | Westernblot reaction box |
-
2010
- 2010-06-22 CN CN2010202316985U patent/CN201724939U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101893624A (en) * | 2010-06-22 | 2010-11-24 | 张子毅 | Westernblot reaction box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: JINAN JINNIU BIOTECHNOLOGY CO., LTD. Assignor: Zhang Ziyi Contract record no.: 2011370000222 Denomination of utility model: Westernblot reaction box Granted publication date: 20110126 License type: Exclusive License Record date: 20110530 |
|
| ASS | Succession or assignment of patent right |
Owner name: JI'NAN JINNIU BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHANG ZIYI Effective date: 20150709 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150709 Address after: 408, room 1, building 322, Shun Feng Road, 250101 hi tech Zone, Shandong, Ji'nan Patentee after: JINAN JINNIU BIOTECHNOLOGY CO., LTD. Address before: 250061 Basic Medicine Institute of Shandong Academy of Medical Sciences, No. ten 89, Lixia District, Ji'nan City, Shandong Province, China Patentee before: Zhang Ziyi |
|
| CX01 | Expiry of patent term |
Granted publication date: 20110126 |
|
| CX01 | Expiry of patent term |