CN101869274A - Autolysis method of spiral seaweed tissue - Google Patents

Abstract

The invention provides an autolysis method of the spiral seaweed tissue, comprising the following technical steps: adding spiral seaweed to water according to the mass ratio of 1:0.05-4; crushing cells and mixing with water; controlling the pH value of spiral seaweed slurry to be in a range of 7-11; adding sodium chloride and enabling the concentration of sodium chloride in the slurry to be 0.3-2.0mol/L; and stirring for 0.5-5.0h at the temperature of 30-65 DEG C, so as to enable the enzyme of the spiral seaweed to perform autolysis reaction with the proteolysis after activation. The obtained spiral seaweed autolysis hydrolysate can be prepared into spiral seaweed powder after spray drying; and the supernate can be directly used for preparing spiral seaweed drinks after centrifugal separation. The spiral seaweed powder prepared by the method in the invention has more comprehensive nutrition and is easy to absorb; the prepared spiral seaweed beverage is nutritious and has favorable taste.

Description

The method of spirulina tissue automatic soup-dissolving

Technical field

The present invention relates to the method for spirulina tissue automatic soup-dissolving.

Background technology

Self-dissolving is the ubiquitous a kind of phenomenon of organism, is the process of being decomposed and being digested by the tissue that cell self enzyme system causes, self-dissolving can produce appreciable impact to organism local flavor, matter structure and constituent; Different with broken wall, the enzyme system that self-dissolving refers to utilize cell interior is hydrolyzed to himself material, and broken wall to be pair cell carry out fragmentation, it mainly acts on is that the enzyme in the cell is discharged, and makes the easier generation of self-dissolving.Spirulina has the characteristics of protein abundance, nutrient equilibrium, is described as one of " human optimal food ".But mainly be to utilize the direct spray-drying of spirulina to make spirulina powder and spiral algae sheet at home, still in the spirulina industry, do not use the precedent of self-dissolving technology, can make spirulina autolyze phenomenon and only be reported in the linoleic acid plus linolenic acid that produces in the breed spirulina process abroad, but not have clear and definite report explanation to utilize the autolysis of spirulina to produce and use.

Experiment shows that spirulina has tangible autolysis, and according to microscopic examination, the spirulina form is not a helical form before the self-dissolving, even its morphological feature of process broken wall still is a trichome of spirulina; But through after the self-dissolving, trichome of spirulina is broken for fragment fully.Simultaneously, by the self-dissolving technology, the enzyme that utilizes spirulina self to contain is hydrolyzed to materials such as spirulina endogenous proteins, materials such as endogenous protein that not only can the enzymolysis spirulina, and compare with utilizing the exogenous protease enzymolysis, after utilization was dissolved in certainly and finishes hydrolysis, the enzyme that need not go out can keep spirulina color and luster and nutritional labeling; Owing to do not introduce exogenous enzymes, reduce the pollution of other material simultaneously.

Summary of the invention

The technical problem to be solved in the present invention provides the method for a kind of spirulina tissue automatic soup-dissolving, becomes a kind of spirulina hydrolysate that is directly used in food production or can be used as meticulous industry raw materials such as medical science, biochemistry after making the spirulina self-dissolving; Also can utilize self-dissolving gained hydrolysate to produce spirulina protein powder and spirulina beverage.

The present invention solves the problems of the technologies described above with following technical scheme:

The step of the method for spirulina tissue automatic soup-dissolving is:

1. spirulina adds water by mass ratio 1: 0.05～4, smashs homogenate to pieces, broken spirulina cells, and mix with water;

2. add the pH buffer and regulate the pH value, and utilize the adjustment agent that pH is finely tuned, control spirulina homogenate pH is 7～11;

3. adding sodium chloride makes that sodium chloride concentration is 0.3-2.0mol/L in the spirulina homogenate;

4. stirred 0.5～5.0 hour at 30～65 ℃, the enzyme that makes spirulina is hydrolyzed to himself protein after activation, thus the autolyze reaction;

5. gained spirulina self-dissolving hydrolysate, the spray-dried spirulina powder that can be made into; If through centrifugation, supernatant can be directly used in the preparation spirulina beverage.

With the spirulina powder that the method for spirulina of the present invention tissue automatic soup-dissolving is made, the spirulina powder that nutrition is made with conventional method more comprehensively, easier absorption; Not only nutrition is good for the spirulina beverage of preparation, and mouthfeel is also fine, has played and has improved edible people's the immunity and the obvious effect of quality of life simultaneously.

The specific embodiment

The present invention relates to the method for spirulina tissue automatic soup-dissolving, particularly utilizing spirulina self enzyme is the method for hydrolysis hydrolysis of protein spirulina.

The step of the method for spirulina of the present invention tissue automatic soup-dissolving is:

1. spirulina adds water by 1: 0.05～4 (mass ratioes), smashs homogenate to pieces, makes the spirulina cells fragmentation, and mixes with water; Used equipment can be high pressure beater, beater, colloid mill etc.

2. add the pH buffer and regulate the pH value, and utilize the adjustment agent that pH is finely tuned, make that spirulina homogenate pH is 7～11.Used buffer can be used with in sodium hydrogen phosphate or the citric acid one or both, and adjusting agent can be hydrochloric acid or NaOH;

3. adding sodium chloride makes that sodium chloride concentration is 0.3-2.0mol/L in the spirulina homogenate, needs before adding to add sodium chloride is water-soluble again;

4. stirred 0.5～5.0 hour at 30～65 ℃, mixing speed is 100～400 rev/mins, and the enzyme of spirulina can be hydrolyzed to himself protein after activation, thus the autolyze reaction;

5. gained spirulina self-dissolving hydrolysate, the spray-dried spirulina powder that can be made into; If through centrifugation, supernatant can be directly used in the preparation spirulina beverage.

Embodiment 1 produces spirulina powder

1, gets fresh spirulina, filter, clean, refilter and obtain spirulina mud through 75 micron pore size filter clothes;

2,20kg spirulina algae mud mixes for 1 liter with clear water, grinds broken wall through colloid mill;

3, regulate pH to 7.0, do not keep pH in the self-dissolving process;

4, adding sodium chloride to concentration is 2.0mol/L;

5, stirred 4 hours down in 30 ℃, mixing speed is 100 rev/mins;

6, self-dissolving rear screw algae hydrolyzate is produced 1.8 kilograms of spirulina powders through spray-drying.

Embodiment 2 produces spirulina beverage

1, gets fresh spirulina, filter, clean, refilter and obtain spirulina mud with 75 micron pore size filter clothes;

2,20g spirulina algae mud mixes with clear water 60g, grinds broken wall through beater;

3, regulate pH to 11.0, do not keep pH in the self-dissolving process;

4, adding sodium chloride to concentration is 0.3mol/L, and solid-liquid ratio is adjusted to 1: 4 (mass ratio) the most at last;

5, stirred 0.5 hour down in 60 ℃, mixing speed is 400 rev/mins;

6, self-dissolving rear screw algae hydrolyzate in 8000 rev/mins centrifugal 20 minutes, 80 milliliters of supernatants, through allotment and suitably dilution, sterilize again and promptly get spirulina beverage.

Embodiment 3

1, gets fresh spirulina, filter, clean, refilter and obtain spirulina mud with 75 micron pore size filter clothes;

2,1g spirulina algae mud mixes with clear water 2g, through high speed beater broken wall;

3, regulate pH to 9.6, do not keep pH in the self-dissolving process;

4, add sodium chloride to 1.0mol/L, stirred 4 hours down in 48 ℃;

6, gained spirulina hydrolyzate is a yellowish-brown after the self-dissolving, and spirulina self-dissolving hydrolyzate is evenly fine and smooth; Find that through microscopic examination the trichome of spirulina body just compares than intact form during broken wall, filament is fragmentation fully.

7, self-dissolving rear screw algae hydrolyzate in 8000 rev/mins centrifugal 20 minutes, discard precipitation, get supernatant.

8, after testing: spirulina self-dissolving supernatant soluble peptide content is 12.9 milligrams of tyrosine equivalents, than self-dissolving spirulina supernatant soluble peptide content (1.7 milligrams of tyrosine equivalents) is not high 7.6 times.

Claims (1)

1. the method for a spirulina tissue automatic soup-dissolving is characterized in that the method step of spirulina tissue automatic soup-dissolving is:

1. spirulina adds water by mass ratio 1: 0.05～4, smashs homogenate to pieces, broken spirulina cells, and mix with water;

2. add the pH buffer and regulate the pH value, and utilize the adjustment agent that pH is finely tuned, control spirulina homogenate pH is 7～11;

3. add sodium chloride, make that sodium chloride concentration is 0.3-2.0mol/L in the spirulina homogenate;

4. stirred 0.5～5.0 hour at 30～65 ℃, the enzyme that makes spirulina is hydrolyzed to himself protein after activation, thus the autolyze reaction;

5. gained spirulina self-dissolving hydrolysate, the spray-dried spirulina powder that can be made into; If through centrifugation, supernatant can be directly used in the preparation spirulina beverage.