CN101858906A - Total air temperature liquid-phase particle incubation and hybridization device with vibration function - Google Patents
Total air temperature liquid-phase particle incubation and hybridization device with vibration function Download PDFInfo
- Publication number
- CN101858906A CN101858906A CN201010159900A CN201010159900A CN101858906A CN 101858906 A CN101858906 A CN 101858906A CN 201010159900 A CN201010159900 A CN 201010159900A CN 201010159900 A CN201010159900 A CN 201010159900A CN 101858906 A CN101858906 A CN 101858906A
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- motor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
Abstract
The invention discloses a total air temperature liquid-phase particle incubation and hybridization device with a vibration function, comprising a box cavity capable of being sealed for insulating heat, a temperature control system, a vibration table, a vibration motor, a stepper motor, a disk and a controller, wherein the temperature control system comprises a refrigeration compressor, a chilled air fan, a hot air fan, a heater and a temperature sensor; the disk is arranged in the box cavity, and a rotating shaft of the disk is connected with the stepper motor; the disk is provided with a plurality of round holes capable of being used for plugging and placing centrifuge tubes along the direction of the radius of the disk vertical to a motor shaft; the vibration motor and the stepper motor are fixed on the vibration table; and the controller is connected with the temperature control system, the stepper motor and the vibration motor and used for controlling the temperature of the box cavity, the rotating speed and the turning direction of the disk and the vibration mode. The device not only can be used foraccurately controlling the temperature to meet various temperature requirements on nano material surface chemistry and biological molecular modification and hybridization, but also can be used for intermittent vibration to ensure that liquid can sufficiently flow so as to disperse nanoparticles and enable the nanoparticles to be uniformly mixed.
Description
Technical field
The present invention relates to biological material analysis quasi-instrument field, the liquid-phase particle that biomacromolecule is modified and molecular hyridization needs that relates in particular to a kind of micro-nano granules surface is hatched and hybrid device.
Background technology
Nano particle is maximum, the most widely used nano material of research in the biomedicine, have character, thereby be widely used in the pathogen detection that replaces the existing chip detection technique, the mark early diagnosis of tumour, the various aspects such as external visual detection of disease such as many uniquenesses such as small-size effect, quantum size effect, surface effect, macro quanta tunnel effect, bulk effects.
Nano particle is applied to biomedical sector, and the primary problem that solves is exactly that nano grain surface is carried out necessary chemistry and the modification of biomacromolecule, as protein, carbohydrate and oligonucleotide probe modification etc.The effect of these biomacromolecules that utilization is modified and other molecule of its specific recognition, the function of this molecule of performance identification.Because the singularity of molecular biology experiment, the chemical reaction of nano grain surface need carry out under specific temperature, yet for such as biomacromolecules such as protein and nucleic acid itself, be easy to be decomposed or owing to the effect of the temperature function that loses activity by the microorganism in the external world; Moreover such as the specific bond ability that has between the biomacromolecules such as antibody and antigen, nucleic acid hybridization, the height of environment temperature of living in it is closely related, so these modifications of nano particle need be able to carried out in the artificial environment of accurate temperature controlling.
On the other hand, traditional solid-phase hybridization is that the DNA with sex change is fixed on the solid matrix, hybridizes with probe again, so be also referred to as blot hybridization on the film, its hybridization is the crossover process of in one plane carrying out, and volume is bigger, needed hybridization solution is many, often places the hybridization bottle of 50mL.In commercially available hybrid heater, impel hybridization solution constantly to mix by the action of gravity of liquid self, evenly hybridization with the rotation that do not stop.Be different from above-mentioned hybridization conditions, the biomacromolecule of nano-material surface is modified and to hybridize required hybridization reaction solution volume many in 100 μ L.Its reaction vessel mostly is the centrifuge tube of 200 μ L～1000 μ L, does not have the special-purpose hybrid device based on the centrifuge tube of 200 μ L～1000 μ L at present on the market.And hybrid pipe is parallel with motor shaft in the at present commercially available molecular hyridization device, and the shockproof function of molecular hyridization device, the hybridization solution self gravitation of small size is less in the rotation crossover process, or the effect of capillarity can not utilize self gravitation to flow, nano particle will soon sedimentation, in addition since the surface effect of nano particle in course of reaction, reunite possibly, thereby cause mixing inhomogeneous, badly influenced the modification or the hybridization efficiency of particle.
Summary of the invention
The objective of the invention is: at the deficiencies in the prior art, a kind of total air temperature liquid-phase particle incubation and hybrid device with vibration function is provided, not only can accurately control to meet all temps demand that nano-material surface chemistry and biomolecule are modified and hybridized temperature, thereby can periodically put upside down the mode that adds the discontinuity vibrations again and make the mobile fully dispersing nanometer particle of liquid, it is mixed.
Technical scheme of the present invention is: a kind of total air temperature liquid-phase particle incubation and hybrid device with vibration function, comprise the temperature control system that to seal heat insulation case chamber and form by refrigeration compressor, chilled air fan, hot blast fan, well heater and temperature sensor, described chilled air fan, hot blast fan and temperature sensor are arranged in the described case chamber, also comprise bumper, vibration motor, motor, disk and controller; Described disk is arranged in the case chamber, and the rotation axis of disk stretches out from case chamber wall by the thermofin in case chamber, link to each other with described motor, be provided with some circular holes that insert the centrifuge tube of various different capabilities along disc radius and perpendicular to the direction of motor shaft on the described disk; Vibration motor and motor are fixedly installed on the bumper, and described controller connects temperature control system, motor and vibration motor, and the temperature in the control box chamber, disc rotation speed, disk turn to and vibrating mode.
Further, the total air temperature liquid-phase particle incubation and the hybrid device of band vibration function also comprise the shock attenuation device that is installed on the bumper base.
Further, the total air temperature liquid-phase particle incubation and the hybrid device of band vibration function also comprise the casing that is arranged on outside, case chamber, and described case chamber, refrigeration compressor, well heater, bumper, motor and vibration motor all are arranged in the casing.
Further, in order to accelerate internal heat dissipating, also be provided with heat abstractor on the described casing.
Further, a side of the close casing in described case chamber is provided with the double thermal insulation glass door, and described double thermal insulation glass door passes described casing and links to each other with the external world, the convenient centrifuge tube of placing.
Further, described motor is a stepper motor.
Further, described disk is provided with and prevents that centrifuge tube from coming off, being wrapped in the outer elastic band of described disk.
Further, the total air temperature liquid-phase particle incubation and the hybrid device of band vibration function also comprise the input/output module that links to each other with controller.
Advantage of the present invention is:
1. temperature range widely is provided, and giving the chemistry and the biomacromolecule modification of nano-material surface and hybridizing provides suitable temperature, has guaranteed the activity of biomacromolecule and the precision of hybridization;
2. be applicable to hatching and hybridizing of small size reactant liquor system, saved reagent and cost;
3. in crossover process, carry out the vibrations of discontinuity, make more fully mixing of liquid and nano material, therefore be specially adapted to the mixing suspended state of nano material in the small size liquid phase environment, guaranteed fully contacting of the reagent in the solution and nano-material surface;
4. the present invention will fill up based on liquid-phase particle and hatch the market vacancy with the hybrid device device.
Description of drawings
Below in conjunction with drawings and Examples the present invention is further described:
Fig. 1 is the inner structure synoptic diagram of the embodiment of the invention.
Wherein: 1 chilled air fan; 2 casees chambeies; 3 double thermal insulation glass doors; 4 refrigeration compressors; 5 controllers; 6 hot blast fans; 7 temperature sensors; 8 disks; 9 elastic bands; 10 casings; 11 shock attenuation devices; 12 bumpers; 13 motors; 14 vibration motors; 15 heat abstractors; 16 well heaters.
Embodiment
Embodiment: the total air temperature liquid-phase particle incubation of the band vibration function of present embodiment and hybrid device comprise case chamber 2 and the casing 10 that is wrapped in outside the case chamber 2, the inwall in case chamber 2 is provided with chilled air fan 1, hot blast fan 6 and temperature sensor 7, is used for the temperature in the case chamber is detected and controls.Chilled air fan 1, hot blast fan 6 and temperature sensor 7 in the case chamber 2, and well heater 16, refrigeration compressor 4 in the casing 10 together constitute temperature control system, the control of controlled device 5, adjustable temperature range is 0 ℃～99.9 ℃, temperature control precision is ± 0.1 ℃, and temperature uniformity is ± 0.2 ℃.
Casing 10 is wrapped in its inside with case chamber 2, case chamber 2 is provided with double thermal insulation glass door 3 near the one side of casing 10, double thermal insulation glass door 3 passes described casing 10 makes case chamber 2 link to each other with the external world, the operator can open this glass door, and the disk 8 that is inserted with various different capabilities centrifuge tubes is put into case chamber 2.
Also be provided with in the case chamber 2 and place centrifuge tube, rotatable disk 8, the rotation axis of disk 8 stretches out from case chamber wall, is connected to the motor 13 that is arranged on outside the case chamber 2, and motor 13 drives disks 8 and rotates, and the motor 13 in the present embodiment adopts stepper motors.On disk 8, be provided with some circular holes that insert the centrifuge tube of various different capabilities, on disk 8, also be provided with and prevent that centrifuge tube from coming off, being wrapped in the elastic band 9 outside the disk along disc radius and perpendicular to the direction of motor shaft.In use, with be equipped with nano material and to be finished or hybridization solution pour centrifuge tube into, in then centrifuge tube being inserted circular hole on the disk with pipe shaft perpendicular to motor shaft, along the direction of disc radius, the disk fixed outside elastic band 9 of centrifuge tube is being housed, is falling from disk 8 when preventing that centrifuge tube from forwarding low level to.
Outside case chamber 2, in the casing 10, be provided with refrigeration compressor 4, controller 5, heat abstractor 15, well heater 16, motor 13, vibration motor 14, bumper 12 and shock attenuation device 11.Motor 13 and vibration motor 14 are fixedly mounted on the bumper 12, and shock attenuation device 11 is installed on the base of bumper 12.Controller 5 connects each electric elements, vibration motor 14 and the motor 13 of temperature control system, and the temperature in the control box chamber, disc rotation speed, disk turn to and vibrating mode.Behind power connection, by the temperature in the temperature sensor detection case chamber 2, according to the temperature value that the user sets, the work of controller control chilled air fan 1, hot blast fan 6, well heater 16, refrigeration compressor 4 is accurately regulated temperature.Simultaneously, the rotating speed that controller is set according to the user and turning to is controlled stepper motor 13 by driver, thus the rotating speed of control disk and turning to; According to the vibrating mode that the user sets, control vibration motor 14, thereby the vibration amplitude and the vibration frequency of control total air temperature liquid-phase particle incubation and hybrid device.The setting that this hybridization instrument is correlated with for convenience, present embodiment also comprises the input/output module (not shown) that links to each other with controller 5, the operator can import on input/output module that temperature value, disc rotation speed, the disk set turn to, vibration amplitude and frequency values, and can show in real time that temperature value, disc rotation speed, the disk of work at present turn to by input/output module, vibration amplitude and frequency values.Motor 13 drives disk 8 slow uniform rotation, and centrifuge tube will periodically be put upside down, and shakes according to certain rules in rotating process.Liquid in the centrifuge tube and nano material will owing to the continuous transform stream of the vibrations of action of gravity and interruption to, reach liquid and fully finish the purpose of modifying and hybridizing with the abundant mixing of nano material.
The above only is the preferred embodiments of the present invention, can not limit scope of the invention process with this, and all simple conversion of doing according to claim of the present invention and description all should still belong to the protection domain that the present invention covers.
Claims (8)
1. total air temperature liquid-phase particle incubation and hybrid device with a vibration function, comprise the temperature control system that to seal heat insulation case chamber (2) and form by refrigeration compressor (4), chilled air fan (1), hot blast fan (6), well heater (16) and temperature sensor (7), described chilled air fan (1), hot blast fan (6) and temperature sensor (7) are arranged in the described case chamber (2), it is characterized in that: also comprise bumper (12), vibration motor (14), motor (13), disk (8) and controller (5); Described disk (8) is arranged in the case chamber (2), and the rotation axis of disk stretches out from case chamber wall by the thermofin of case chamber (2), link to each other with described motor (13), described disk (8) is gone up along disc radius and perpendicular to the direction of motor shaft and is provided with some circular holes that insert various different capabilities centrifuge tubes; Vibration motor (14) and motor (13) are fixedly installed on the bumper (12), and described controller (5) connects temperature control system, motor (13) and vibration motor (14), and temperature, disc rotation speed, disk in control box chamber (2) turn to and vibrating mode.
2. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in the claim 1, it is characterized in that: also comprise the shock attenuation device (11) that is installed on bumper (12) base.
3. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in claim 1 or 2, it is characterized in that: also comprise being arranged on the outside casing (10) in case chamber (2), described case chamber (2), refrigeration compressor (4), well heater (16), bumper (12), motor (13) and vibration motor (14) all are arranged in the casing (10).
4. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in the claim 3, it is characterized in that: also be provided with heat abstractor (15) on the described casing (10).
5. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in the claim 3, it is characterized in that: a side of the close casing (10) in described case chamber (2) is provided with double thermal insulation glass door (3), and described double thermal insulation glass door (3) passes described casing (10) and links to each other with the external world.
6. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in any in the claim 1 to 5, it is characterized in that: described motor (10) is a stepper motor.
7. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in any in the claim 1 to 5, it is characterized in that: described disk (8) is provided with and prevents that centrifuge tube from coming off, being wrapped in the outer elastic band (9) of described disk (8).
8. according to the total air temperature liquid-phase particle incubation and the hybrid device of the band vibration function described in any in the claim 1 to 5, it is characterized in that: also comprise the input/output module that links to each other with controller (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010159900.2A CN101858906B (en) | 2010-04-29 | 2010-04-29 | Total air temperature liquid-phase particle incubation and hybridization device with vibration function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010159900.2A CN101858906B (en) | 2010-04-29 | 2010-04-29 | Total air temperature liquid-phase particle incubation and hybridization device with vibration function |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101858906A true CN101858906A (en) | 2010-10-13 |
| CN101858906B CN101858906B (en) | 2014-01-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010159900.2A Expired - Fee Related CN101858906B (en) | 2010-04-29 | 2010-04-29 | Total air temperature liquid-phase particle incubation and hybridization device with vibration function |
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| Country | Link |
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| CN (1) | CN101858906B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102638959A (en) * | 2012-04-11 | 2012-08-15 | 苏州怡信光电科技有限公司 | Electric cabinet for engraving and milling machine |
| CN102899248A (en) * | 2012-10-25 | 2013-01-30 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN103992945A (en) * | 2014-05-19 | 2014-08-20 | 东南大学 | Constant-temperature incubation and hybridization device |
| CN107012069A (en) * | 2017-06-07 | 2017-08-04 | 浙江师范大学 | Blood Bacteria Culture detection means and blood Bacteria Culture detector |
| CN108802403A (en) * | 2018-07-28 | 2018-11-13 | 湖南中瑞互信医疗科技有限公司 | A kind of constant-temperature incubation shaking table |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102638959A (en) * | 2012-04-11 | 2012-08-15 | 苏州怡信光电科技有限公司 | Electric cabinet for engraving and milling machine |
| CN102899248A (en) * | 2012-10-25 | 2013-01-30 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN102899248B (en) * | 2012-10-25 | 2014-11-26 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN103992945A (en) * | 2014-05-19 | 2014-08-20 | 东南大学 | Constant-temperature incubation and hybridization device |
| CN107012069A (en) * | 2017-06-07 | 2017-08-04 | 浙江师范大学 | Blood Bacteria Culture detection means and blood Bacteria Culture detector |
| CN108802403A (en) * | 2018-07-28 | 2018-11-13 | 湖南中瑞互信医疗科技有限公司 | A kind of constant-temperature incubation shaking table |
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| Publication number | Publication date |
|---|---|
| CN101858906B (en) | 2014-01-15 |
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