CN201574148U - Total-temperature miniature blending incubation and hybridization instrument - Google Patents
Total-temperature miniature blending incubation and hybridization instrument Download PDFInfo
- Publication number
- CN201574148U CN201574148U CN 200920283960 CN200920283960U CN201574148U CN 201574148 U CN201574148 U CN 201574148U CN 200920283960 CN200920283960 CN 200920283960 CN 200920283960 U CN200920283960 U CN 200920283960U CN 201574148 U CN201574148 U CN 201574148U
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- temperature
- hybridization
- hybridization instrument
- blending incubation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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Abstract
The utility model provides a total-temperature miniature blending incubation and hybridization instrument, which comprises a closed box cavity (2), a hot air fan (6), a cold air fan (1), a refrigeration compressor (4), a temperature sensor (11) and a heating constant-temperature system (12), wherein the hot air fan (6), the cold air fan (1) and the temperature sensor (11) are disposed inside the box cavity (2). The total-temperature miniature blending incubation and hybridization instrument is characterized by further comprising a motor (10) and a circular disc (8), the circular disc (8) is disposed inside the box cavity (2) and connected with the motor (10), and a circular hole capable of being inserted with a centrifuge tube is arranged on the circular disc (8) in a direction perpendicular to a motor shaft. The total-temperature miniature blending incubation and hybridization instrument provides wider temperature range, provides suitable temperature for modification and hybridization of chemical and biological macromolecules on surfaces of nanometer materials, guarantees activity and hybridization precision of the biological macromolecules, is particularly suitable for the nanometer materials to be blended and suspended in a small-volume liquid-phase environment, and guarantees modification uniformity of the surfaces of the nanometer materials.
Description
Technical field
The utility model belongs to biological material analysis quasi-instrument field, particularly has the biomacromolecule on the micro-nano granules surface of requirement to modify to temperature and the needed full temperature microbody volume blending of molecular hybridization is exhaled and educated and hybridization instrument.
Background technology
Nano particle is maximum, the most widely used nano material of research in the biomedicine, and the character of many uniquenesses is arranged.Research at present more has carbon, silicon, quantum dot and superparamagnetic nanomaterial etc.These materials have as characteristics such as small-size effect, quantum size effect, surface effects, macro quanta tunnel effect, volume effects, are widely used in the pathogen detection that replaces the existing chip detection technique, the mark early diagnosis of tumour, all respects such as external visual detection of disease.
Nano particle biomedical sector be to be applied to, just must necessary chemistry and the modification of biomacromolecule be carried out, as protein, carbohydrate and oligonucleotide probe modification etc. to nano grain surface.The effect of these biomacromolecules that utilization is modified and other molecule of its specific recognition, the function of this molecule of performance identification.Because the singularity of Bioexperiment, the chemical reaction of nano grain surface needs certain temperature; To biomacromolecule itself, as protein and nucleic acid etc., can be by extraneous microbiological degradation or owing to the effect of the temperature function that loses activity; The perhaps specific combination ability between the biomacromolecule, as antibody and antigen, nucleic acid hybridization etc., the height of temperature are in conjunction with whether deciding factor.Therefore these modifications of nano particle need be able to carried out in the artificial environment of accurate temperature controlling.
At present, in the prior art, chip, Southern or the Northern hybridization of carrying out based on slide and Hybond membrane is crossover process in one plane, the bigger needed hybridization solution of its volume is many, place hybridization bottle (50mL), in commercially available hybrid heater, impel hybridization solution constantly to mix by the gravity of liquid self, evenly hybridization with the rotation that do not stop.Be different from above-mentioned hybridization conditions, the biomacromolecule of nano-material surface is modified and how hybridizes required hybridization reaction solution volume at 20-100 μ L, and its reaction vessel mostly is the centrifuge tube of 200 μ L-1000 μ L.There is not special-purpose hybrid device at present on the market based on the centrifuge tube of 200 μ L-1000 μ L, and hybrid pipe is parallel with motor shaft in commercially available Hybridization Oven, the hybridization solution self gravitation of small volume is little in the rotation crossover process, or the effect of capillarity can not utilize self gravitation to flow, nano particle will soon sedimentation, thereby cause mixing inhomogeneous, badly influenced that particulate is modified or hybridization efficiency.
The utility model content
The purpose of this utility model is exactly the deficiency that will overcome commercially available instrument, providing a kind of both can accurately control to meet all temps demand that nano-material surface chemistry and biomolecules are modified and hybridized temperature, can make liquid-flow fully again, make uniform full temperature micro volume blending incubation of liquid-phase mixing and hybridization instrument.
The purpose of this utility model is achieved in that a kind of full temperature micro volume blending incubation and hybridization instrument, comprise closed case chamber, Hot-air fan, thermantidote, refrigeration compressor, temperature sensor and heating constant-temperature system, described Hot-air fan, thermantidote and temperature sensor are arranged in the described case chamber, also comprise motor and disk, described disk is arranged in the described case chamber, be connected with described motor, the direction perpendicular to motor shaft on the described disk is provided with the round hole that can insert centrifuge tube.Bonded heating constant-temperature system on the case chamber is changed by the temperature sensor sense temperature, and by chilled air fan and hot blast fan the temperature in the chamber is controlled, and temperature range is 0 ℃～99.9 ℃, and temperature control precision is ± 0.1 ℃, and temperature uniformity is ± 0.2 ℃.
Be arranged on outermost casing in order to make the compactness rational in infrastructure of the utility model full temperature micro volume blending incubation and hybridization instrument, also to comprise, described case chamber, refrigeration compressor, heating constant-temperature system and motor all are arranged in the described casing.
Convenient in order to place centrifuge tube, described case chamber is provided with the double thermal insulation glass door, and described double thermal insulation glass door passes described casing and links to each other with the external world.
In order to make the utility model product internal heat dissipating fast, also be provided with heat abstractor in the described casing.
For easy to operate, also comprise device control unit in the utility model product, device control unit links to each other with all electric elements in the product, temperature controllable and motor speed, described device control unit is arranged on the described casing.
In order to make disc rotation speed 0-60rpm controlled, described motor is a DC speed-reducing.
Fall from disk when preventing that centrifuge tube from forwarding low level to, described disk is provided with the elastic band that prevents that centrifuge tube from coming off.
During use, the centrifuge tube that nano material and to be finished or hybridization solution are housed is inserted disk, fix with elastic band, behind power connection, adjustment control as required, controlled temperature and rotating speed (0～60rpm), the slow uniform rotation of driven by motor disk, centrifuge tube will periodically be put upside down.Liquid in the pipe and nano material will owing to the continuous transform stream of gravity to, fully finish the purpose of modifying and hybridizing to reach liquid with the abundant mixing of nano material.
The beneficial effects of the utility model are:
Full temperature micro volume blending incubation of the present utility model and hybridization instrument compared with prior art have following characteristics:
One, the utility model product provides temperature range widely, modifies for the chemistry of nano-material surface and biomacromolecule, reaches hybridization suitable temperature is provided, and has guaranteed the activity of biomacromolecule and the precision of hybridization;
Two, the utility model product is applicable to hatching of small volume reaction liquid system and hybridizes, and has saved reagent and cost; Be specially adapted to the maintenance mixing suspended state of nano material in the small volume liquid phase environment, guaranteed fully contacting of the reagent in the solution and nano-material surface, guaranteed the homogeneity that nano-material surface is modified;
Three, the utility model product is filled up the market vacancy based on micro volume blending incubation and hybridization instrument.
Description of drawings
Fig. 1 is the structural representation of the utility model full temperature micro volume blending incubation and hybridization instrument.
Embodiment
Mode below by embodiment is elaborated to technical solutions of the utility model, but protection domain of the present utility model is not limited to described embodiment.
As shown in Figure 1, a kind of full temperature micro volume blending incubation and hybridization instrument, comprise closed case chamber 2, Hot-air fan 6, thermantidote 1, refrigeration compressor 4, temperature sensor 11 and heating constant-temperature system 12, described Hot-air fan 6, thermantidote 1 and temperature sensor 11 are arranged in the described case chamber 2, also comprise DC speed-reducing 10 and disk 8, described disk 8 is arranged in the described case chamber 2, be connected with described DC speed-reducing 10, direction perpendicular to motor shaft on the described disk 8 is provided with the round hole that can insert centrifuge tube, described disk 8 is provided with the elastic band 9 that prevents that centrifuge tube from coming off, during use, the centrifuge tube that nano material and to be finished or hybridization solution are housed is inserted in the round hole on the disk, centrifuge tube is inserted in the disk in the mode of pipe shaft perpendicular to motor shaft, the outside is fixing with elastic band 9, falls from disk when preventing that centrifuge tube from forwarding low level to; Be combined with heating constant-temperature system 12 on the case chamber 2, control by the temperature in the variation of temperature sensor 11 sense temperature, chilled air fan 1 and 6 pairs of chambeies of hot blast fan, temperature range is 0 ℃～99.9 ℃, and temperature control precision is ± 0.1 ℃, and temperature uniformity is ± 0.2 ℃;
Also comprise being arranged on outermost casing 7, described case chamber 2, refrigeration compressor 4 and DC speed-reducing 10 all are arranged in the described casing 7; Described case chamber 2 is provided with double thermal insulation glass door 3, and described double thermal insulation glass door 3 passes described casing 7 and links to each other with the external world; Also be provided with heat abstractor 13 in the described casing 7; Also comprise device control unit 5, described device control unit 5 is arranged on the described casing 7.
Behind power connection, adjustment control 5 as required, controlled temperature and rotating speed, and DC speed-reducing 10 drives disk 8 slow uniform rotation, and centrifuge tube will periodically be put upside down.Liquid in the pipe and nano material will owing to the continuous transform stream of gravity to, fully finish the purpose of modifying and hybridizing to reach liquid with the abundant mixing of nano material.
Claims (7)
1. full temperature micro volume blending incubation and hybridization instrument, comprise closed case chamber (2), Hot-air fan (6), thermantidote (1), refrigeration compressor (4), temperature sensor (11) and heating constant-temperature system (12), described Hot-air fan (6), thermantidote (1) and temperature sensor (11) are arranged in the described case chamber (2), it is characterized in that: also comprise motor (10) and disk (8), described disk (8) is arranged in the described case chamber (2), be connected with described motor (10), the direction that described disk (8) is gone up perpendicular to motor shaft is provided with the round hole that can insert centrifuge tube.
2. full temperature micro volume blending incubation according to claim 1 and hybridization instrument, it is characterized in that: also comprise being arranged on outermost casing (7), described case chamber (2), refrigeration compressor (4), heating constant-temperature system (12) and motor (10) all are arranged in the described casing (7).
3. full temperature micro volume blending incubation according to claim 2 and hybridization instrument is characterized in that: described case chamber (2) is provided with double thermal insulation glass door (3), and described double thermal insulation glass door (3) passes described casing (7) and links to each other with the external world.
4. full temperature micro volume blending incubation according to claim 2 and hybridization instrument is characterized in that: also be provided with heat abstractor (13) in the described casing (7).
5. full temperature micro volume blending incubation according to claim 2 and hybridization instrument is characterized in that: also comprise device control unit (5), described device control unit (5) is arranged on the described casing (7).
6. according to described full temperature micro volume blending incubation of arbitrary claim and hybridization instrument in the claim 1～5, it is characterized in that: described motor (10) is DC speed-reducing.
7. according to described full temperature micro volume blending incubation of arbitrary claim and hybridization instrument in the claim 1～5, it is characterized in that: described disk (8) is provided with the elastic band (9) that prevents centrifuge tube and come off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920283960 CN201574148U (en) | 2009-12-14 | 2009-12-14 | Total-temperature miniature blending incubation and hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920283960 CN201574148U (en) | 2009-12-14 | 2009-12-14 | Total-temperature miniature blending incubation and hybridization instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201574148U true CN201574148U (en) | 2010-09-08 |
Family
ID=42694024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200920283960 Expired - Lifetime CN201574148U (en) | 2009-12-14 | 2009-12-14 | Total-temperature miniature blending incubation and hybridization instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201574148U (en) |
-
2009
- 2009-12-14 CN CN 200920283960 patent/CN201574148U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: NAIJING ZHONGKE SHENGUANG TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SOWTHEAST UNIV. Effective date: 20141208 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 210096 NANJING, JIANGSU PROVINCE TO: 210038 NANJING, JIANGSU PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20141208 Address after: Xinghe Nanjing economic and Technological Development Zone Road Qixia District of Nanjing City, Jiangsu province 210038 No. 18 Patentee after: Naijing Zhongke Shenguang Technology Co., Ltd. Address before: 210096 No. four archway, Xuanwu District, Jiangsu, Nanjing 2 Patentee before: Southeast University |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100908 |