CN101856276A - Method for collecting semen from cynomolgus monkey - Google Patents
Method for collecting semen from cynomolgus monkey Download PDFInfo
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- CN101856276A CN101856276A CN201010188484A CN201010188484A CN101856276A CN 101856276 A CN101856276 A CN 101856276A CN 201010188484 A CN201010188484 A CN 201010188484A CN 201010188484 A CN201010188484 A CN 201010188484A CN 101856276 A CN101856276 A CN 101856276A
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- penis
- semen
- machin
- cynomolgus monkey
- collecting
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Abstract
The invention discloses a method for collecting semen from a cynomolgus monkey. The method adopts a physiological and pharmacological electronic stimulation instrument, two self-made penis electrode devices, a strictly sterilized centrifugal tube, a pipette and a thermos flask; and the semen collecting method adopts a non-narcotic restraint method. An operator collects the semen through the following steps of: 1, soothing the cynomolgus monkey to stabilize the mood thereof; 2, sterilizing the appliances and the penis of the cynomolgus monkey; and 3, turning on a power supply of the physiological and pharmacological electronic stimulation instrument to perform simulation so as to collect the semen. The method has the advantages that the method can be used for artificial insemination of the cynomolgus monkey and normal-temperature and freezing preservation of the semen, can fully play the reproductive efficiency of excellent male cynomolgus monkeys, can preserve genetic genes of the excellent cynomolgus monkeys and meanwhile provides experience reference for the semen collecting technology for other rare endangered primates.
Description
Technical field
The invention belongs to biological technical field, specifically is a kind of method for collecting semen from cynomolgus monkey.
Background technology
So far and find no same or analogous report with the present invention.
Summary of the invention
The purpose of this invention is to provide a kind of method for collecting semen from cynomolgus monkey.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
A kind of method for collecting semen from cynomolgus monkey, this method adopts one in physiological and pharmacological electronic stimulation instrument, two of self-made penis electrode devices; The centrifuge tube that strict sterilization is crossed; Pipettor, one in thermos flask, the semen collection method is used non-narcotic Baoding method, and the concrete operations step is as follows:
1) by 1-2 name personnel public monkey extremity in the cage are caught, fully exposure of genitals is suitably pacified machin, to stablize its emotion;
2) operator are two people, wherein a people shampoos penis and places electrode, another people takes centrifuge tube to connect seminal fluid, and be responsible for adjusting and switch physiological and pharmacological electronic stimulation instrument, operator clean machin penis portion for several times with normal saline wet towel or gauze, with cleaning machin penis and enhancing electric conductivity, with two self-made penis electrode devices, one connects positive pole, another connects negative pole, be individually fixed in the front portion of machin body of penis and the rear portion of body of penis, and adjust the annulus size of self-made penis electrode device according to the size of machin penis;
3) connect physiological and pharmacological electronic stimulation instrument power supply, stimulate, rise to voltage be 20V, whenever, take turns stimulation and all carry out 2 times and stimulate, each stimulation time is 3～10s, and be 5s blanking time, heighten 8 volts to voltage behind the 30s at interval at every seminal fluid of still not adopting after taking turns stimulation, a beginning new round stimulates, and requirement voltage is adjusted from low to high during semen collection, stimulator instrument setup parameter is, ripple is wide to be 10ms, and voltage 20V is between the 56V, break time 20ms, voltage source is 1.5mA, adopts seminal fluid;
4) with seminal fluid collecting in aseptic centrifuge tube, with liquid-transfering gun liquid seminal fluid is moved on in the little centrifuge tube,, be placed in the thermos flask of 25～28 ℃ of suitable quantity of water of prepackage and transport laboratory back by test requirements document dilution with diluent.The semen collection frequency is that public monkey is noted suitable extra-nutrition maximum 2 times weekly.
Above-mentioned self-made penis electrode device is to adopt common copper wire to make scalable annular electrode device.
The invention has the beneficial effects as follows:
Can be used for machin artificial insemination and semen at normal temperature and freezing preservation, can give full play to the reproductive efficiency of good male machin, also can preserve the gene of good machin, use for reference for other rare primate semen collection technical research in imminent danger provide experience simultaneously.
The specific embodiment
Below in conjunction with embodiment the present invention is further retouched art.
Embodiment
Choose 5-8 the male machin of adult healthy, by 2 poultry raisers public monkey extremity are caught, fully exposure of genitals is suitably pacified machin, to stablize its emotion.
A poultry raiser shampoos penis and places electrode, another technical staff takes 50 milliliters of sterilization centrifuge tubes to be responsible for connecing seminal fluid, and be responsible for adjusting and switch physiological and pharmacological electronic stimulation instrument, operator clean machin penis portion for several times with normal saline wet towel or gauze, with cleaning machin penis and enhancing electric conductivity, with two self-made penis electrode devices, one connects positive pole, another connects negative pole, be individually fixed in the front portion of machin body of penis and the rear portion of body of penis, and adjust the annulus size of self-made penis electrode device according to the size of machin penis.
The technical staff connects physiological and pharmacological electronic stimulation instrument power supply, stimulates, rise to voltage be 20V, whenever, take turns stimulation and all carry out 2 times and stimulate, each stimulation time is 3～10s, and be 5s blanking time, heighten 8 volts to voltage behind the 30s at interval at every seminal fluid of still not adopting after taking turns stimulation, a beginning new round stimulates, and requirement voltage is adjusted from low to high during semen collection, stimulator instrument setup parameter is, ripple is wide to be 10ms, and voltage 20V is between the 56V, break time 20ms, voltage source is 1.5mA, until adopting seminal fluid.
With seminal fluid collecting in 50 milliliters the sterilization centrifuge tubes in, with liquid-transfering gun liquid seminal fluid is moved on in 15 milliliters of little centrifuge tubes of sterilization, do 20 times of dilutions with the machin semen diluent, be placed in the thermos flask of 25～28 ℃ of suitable quantity of water of prepackage and transport laboratory back and carry out the seminal fluid routine examination.The semen collection frequency is that public monkey is noted suitable extra-nutrition maximum 2 times weekly.
The machin seminal fluid of gathering can be used for carrying out artificial insemination to female monkey, also can be used for freezing preservation.
Claims (2)
1. a method for collecting semen from cynomolgus monkey is characterized in that, this method adopts one in physiological and pharmacological electronic stimulation instrument, two of self-made penis electrode devices; The centrifuge tube that strict sterilization is crossed; Pipettor, one in thermos flask, the semen collection method is used non-narcotic Baoding method, and the concrete operations step is as follows:
1) by 1-2 name personnel public monkey extremity in the cage are caught, fully exposure of genitals is suitably pacified machin, to stablize its emotion;
2) operator are two people, wherein a people shampoos penis and places electrode, another people takes centrifuge tube to connect seminal fluid, and be responsible for adjusting and switch physiological and pharmacological electronic stimulation instrument, operator clean machin penis portion for several times with normal saline wet towel or gauze, with cleaning machin penis and enhancing electric conductivity, with two self-made penis electrode devices, one connects positive pole, another connects negative pole, be individually fixed in the front portion of machin body of penis and the rear portion of body of penis, and adjust the annulus size of self-made penis electrode device according to the size of machin penis;
3) connect physiological and pharmacological electronic stimulation instrument power supply, stimulate, rise to voltage be 20V, whenever, take turns stimulation and all carry out 2 times and stimulate, each stimulation time is 3～10s, and be 5s blanking time, heighten 8 volts to voltage behind the 30s at interval at every seminal fluid of still not adopting after taking turns stimulation, a beginning new round stimulates, and requirement voltage is adjusted from low to high during semen collection, stimulator instrument setup parameter is, ripple is wide to be 10ms, and voltage 20V is between the 56V, break time 20ms, voltage source is 1.5mA, adopts seminal fluid;
4) with seminal fluid collecting in aseptic centrifuge tube, with liquid-transfering gun liquid seminal fluid is moved on in the little centrifuge tube,, be placed in the thermos flask of 25～28 ℃ of suitable quantity of water of prepackage and transport laboratory back by test requirements document dilution with diluent.The semen collection frequency is that public monkey is noted suitable extra-nutrition maximum 2 times weekly.
2. a kind of method for collecting semen from cynomolgus monkey according to claim 1 is characterized in that, described self-made penis electrode device is to adopt common copper wire to make scalable annular electrode device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010188484A CN101856276A (en) | 2010-06-01 | 2010-06-01 | Method for collecting semen from cynomolgus monkey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010188484A CN101856276A (en) | 2010-06-01 | 2010-06-01 | Method for collecting semen from cynomolgus monkey |
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| CN101856276A true CN101856276A (en) | 2010-10-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201010188484A Pending CN101856276A (en) | 2010-06-01 | 2010-06-01 | Method for collecting semen from cynomolgus monkey |
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| CN (1) | CN101856276A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784214A (en) * | 2012-10-30 | 2014-05-14 | 苏州药明康德新药开发有限公司 | Monkey semen collection method |
| CN104758082A (en) * | 2015-03-06 | 2015-07-08 | 华南农业大学 | Semen collection method of macaque |
| CN106333762A (en) * | 2016-09-20 | 2017-01-18 | 成都测迪森生物科技有限公司 | Semen collection cup for chicken |
-
2010
- 2010-06-01 CN CN201010188484A patent/CN101856276A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 《中国畜牧兽医学会动物解剖学及组织胚胎学分会第十五次学术研讨会》 20081231 黎宗强等 食蟹猴阴茎电刺激采精及精液特征的初步研究 220-224 , 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784214A (en) * | 2012-10-30 | 2014-05-14 | 苏州药明康德新药开发有限公司 | Monkey semen collection method |
| CN103784214B (en) * | 2012-10-30 | 2016-05-25 | 苏州药明康德新药开发股份有限公司 | The method of monkey semen collection |
| CN104758082A (en) * | 2015-03-06 | 2015-07-08 | 华南农业大学 | Semen collection method of macaque |
| CN106333762A (en) * | 2016-09-20 | 2017-01-18 | 成都测迪森生物科技有限公司 | Semen collection cup for chicken |
| CN106333762B (en) * | 2016-09-20 | 2018-05-29 | 南京农业大学 | One breeder semen collection cup |
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Open date: 20101013 |