CN103784214A - Monkey semen collection method - Google Patents
Monkey semen collection method Download PDFInfo
- Publication number
- CN103784214A CN103784214A CN201210422817.9A CN201210422817A CN103784214A CN 103784214 A CN103784214 A CN 103784214A CN 201210422817 A CN201210422817 A CN 201210422817A CN 103784214 A CN103784214 A CN 103784214A
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- China
- Prior art keywords
- monkey
- penis
- baoding
- seminal fluid
- described step
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000282693 Cercopithecidae Species 0.000 title claims abstract description 73
- 210000000582 semen Anatomy 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 24
- 210000003899 penis Anatomy 0.000 claims abstract description 37
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 230000001404 mediated effect Effects 0.000 claims description 4
- 230000003340 mental effect Effects 0.000 claims description 3
- 230000002085 persistent effect Effects 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 8
- 230000006378 damage Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
The invention discloses a monkey semen collection method. The monkey semen collection method comprises the steps of 1 fixing a monkey; 2 processing the monkey penis; 3 using an electrical stimulator to stimulate the monkey penis to enable the monkey penis to produce semen; 4 collecting the semen. The monkey semen collection method has the advantages of being simple in operation and not prone to pollution, causing small damage and the like, has wide application value and promotes medical research development.
Description
Technical field
The present invention relates to a kind of method of semen collection, particularly relate to a kind of method of monkey semen collection.
Background technology
Monkey is important animal for research, and its evolution degree is the highest, is the ideal animals close to the mankind, more similar to the mankind on physiology compared with other animals, is to show the unique kinds of the mankind to tested material reaction.Utilize clear and mischievous person's liquid can judge whether monkey is sexual maturity, for some researchs provide information.Meanwhile, also can utilize compound composition, content in clear and mischievous person's liquid to carry out the research of some bioavailability.But because of the physiological structure of monkey, it is carried out to semen collection and have certain difficulty, application is not also a lot of at present.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method of monkey semen collection.Adopt the method, can arrive clear and mischievous person's liquid by Quick Acquisition, be beneficial to research.
For solving the problems of the technologies described above, the method for monkey semen collection of the present invention, comprises step:
1) Baoding monkey;
2) to the processing of monkey penis;
3) stimulate monkey penis with electrostimulator, make it produce seminal fluid;
4) collect seminal fluid.
In Baoding of described step 1), position, Baoding comprises: the extremity of monkey, when Baoding, take and carve the breath state of observing monkey, extremity situation and the mental status.As used monkey chair Baoding monkey, allow monkey be sitting on monkey chair, extremity are fixed on monkey chair with gauze, expose monkey penis, guarantee the comfortable of monkey, can fully expose again the penis of monkey, and meanwhile, the extremity of monkey can not hurt people yet.
Described step 2) in, when monkey penis is processed, the gauze parcel penis of selecting 0.9% normal saline to soak, is beneficial to and conducts electricity and guarantee that penis is injury-free.
In described step 3), stimulate the step of monkey penis to be: to the gauze for wrapping up penis, it is per second that electrostimulator is set to 10 to 30 pulses by 2 electrode contacts of electrostimulator, and the persistent period is 1 to 3 millisecond.The voltage of electrostimulator can be mediated according to the reaction of monkey, as mediated between 0 to 50 volt, and should, from minimum voltage, increase gradually as required, until monkey is penetrated seminal fluid.
In described step 4), the container of collection is the container that width, the degree of depth that opening diameter is greater than monkey penis are greater than the length of monkey penis, as 10mL centrifuge tube.Use this container, both guaranteed that seminal fluid is easy to collect and can collect completely, be unlikely to again contaminated.
In the collection seminal fluid of described step 4), the interval of twice collection seminal fluid at least 24 hours.
Acquisition method of the present invention, have simple to operate, damage little, be difficult for the advantage such as pollutions, be with a wide range of applications, the development of promotion medical research.
Accompanying drawing explanation
Below in conjunction with accompanying drawing and the specific embodiment, the present invention is further detailed explanation:
Fig. 1 is the state diagram of monkey while being fixed on monkey chair.
The specific embodiment
Clear and mischievous person's liquid acquisition method of the present invention, step is as follows:
1) Baoding monkey
Check after monkey numbering (No. ID), utilize necklace that monkey is fixed on to (as shown in Figure 1) on monkey chair, use gauze fixing limbs, make it positive in the face of picker, be sitting on monkey chair, to expose monkey penis.
When Baoding, note the elasticity in Baoding, and breath state, extremity situation and the mental status of moment observation monkey, guarantee that monkey can freely breathe, extremity degree of tightness is moderate.
2) to the processing of monkey penis
The gauze parcel penis middle part of soaking with 0.9% normal saline, exposes monkey glans penis, and the both sides (along penis direction) of gauze can be by the electric shock of electrostimulator folder folder simultaneously.
3) electrostimulator stimulates monkey penis, produces seminal fluid
Connect electrostimulator (JL-C2 electrostimulator, Shanghai Jia Longjiao instrument factory manufacture), open instrument, by 2 electrode contacts of electrostimulator to gauze.It is per second that electrostimulator is set to 10 to 30 pulses, and the persistent period is 1 to 3 millisecond.The voltage of electrostimulator is mediated between 0 to 50 volt, and from minimum voltage, increases gradually as required, until monkey is penetrated seminal fluid.
4) reconcile when electrostimulator, picker, by collection container (as 10mL centrifuge tube) being enclosed within to this acquisition mode on the glans penis of penis, is ready at all times to collect seminal fluid.Wherein, the individual variation that the collecting amount of this seminal fluid is looked monkey is from 0.1g to 3g and different.Meanwhile, if need to repeatedly collect, collect the interval at least 24 hours of seminal fluid twice.
Carry out according to the method described above collecting semen, tool has the following advantages:
1) use monkey chair Baoding monkey, be beneficial to collection, monkey can not hurt people yet simultaneously;
2) by the gauze conductive electric current soaking, few to monkey injury of penis, draw materials conveniently, be beneficial to operation;
3) seminal fluid collecting flows directly in centrifuge tube, avoids waiting contacting of polluter with operator, is difficult for producing and pollutes, and is difficult for waste;
Therefore, the present invention can be widely used in medical science, the research of toxicology etc.Wherein, need to use sexual maturity monkey study the research that maybe needs to detect compounds content in seminal fluid for some, the present invention is a kind of well acquisition method.
Claims (10)
1. a method for monkey semen collection, is characterized in that, comprises step:
1) Baoding monkey;
2) to the processing of monkey penis;
3) stimulate monkey penis with electrostimulator, make it produce seminal fluid;
4) collect seminal fluid.
2. the method for claim 1, is characterized in that: in Baoding of described step 1), position, Baoding comprises: the extremity of monkey, when Baoding, take and carve the breath state of observing monkey, extremity situation and the mental status.
3. the method for claim 1, is characterized in that: in Baoding of described step 1), use monkey chair Baoding monkey, allow monkey be sitting on monkey chair, extremity are fixed on monkey chair with gauze, expose monkey penis.
4. the method for claim 1, is characterized in that: described step 2) in, when monkey penis is processed, the gauze parcel penis of selecting 0.9% normal saline to soak.
5. method as claimed in claim 4, is characterized in that: in described gauze parcel penis, the gauze parcel penis middle part that adopts 0.9% normal saline to soak, exposes monkey glans penis, and the both sides of gauze can be by the electric shock of electrostimulator folder folder simultaneously.
6. the method for claim 1, is characterized in that: in described step 3), stimulate the step of monkey penis to be:
By 2 electrode contacts of electrostimulator, to the gauze for wrapping up penis, it is per second that electrostimulator is set to 10 to 30 pulses, and the persistent period is 1 to 3 millisecond; The voltage of electrostimulator is mediated between 0 to 50 volt, and from minimum voltage, increases gradually as required, until monkey is penetrated seminal fluid.
7. the method for claim 1, is characterized in that: in described step 4), the container of collection is the container that width, the degree of depth that opening diameter is greater than monkey penis are greater than the length of monkey penis.
8. method as claimed in claim 7, is characterized in that: the container of described collection is 10mL centrifuge tube.
9. the method for claim 1, is characterized in that: in described step 4), collect the mode of seminal fluid and be picker by the container set of collecting on the glans penis of penis.
10. the method for claim 1, is characterized in that: in the collection seminal fluid of described step 4), and the interval of twice collection seminal fluid at least 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210422817.9A CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210422817.9A CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103784214A true CN103784214A (en) | 2014-05-14 |
| CN103784214B CN103784214B (en) | 2016-05-25 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210422817.9A Active CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103784214B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758082A (en) * | 2015-03-06 | 2015-07-08 | 华南农业大学 | Semen collection method of macaque |
| CN107669370A (en) * | 2016-08-02 | 2018-02-09 | 西南大学 | The optimization method of one elite stand musk deer electro-ejaculation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856276A (en) * | 2010-06-01 | 2010-10-13 | 广西大学 | Method for collecting semen from cynomolgus monkey |
| CN101869512A (en) * | 2010-06-12 | 2010-10-27 | 昆明亚灵生物科技有限公司 | Method for collecting semen of primates of macaque by electro stimulation |
-
2012
- 2012-10-30 CN CN201210422817.9A patent/CN103784214B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856276A (en) * | 2010-06-01 | 2010-10-13 | 广西大学 | Method for collecting semen from cynomolgus monkey |
| CN101869512A (en) * | 2010-06-12 | 2010-10-27 | 昆明亚灵生物科技有限公司 | Method for collecting semen of primates of macaque by electro stimulation |
Non-Patent Citations (3)
| Title |
|---|
| 侯进怀等: "《太行山猕猴电刺激采精试验》", 《河南师范大学学报（自然科学版）》, vol. 26, no. 1, 28 February 1998 (1998-02-28), pages 75 - 77 * |
| 杨上川等: "《一种改进的阴茎电刺激采精法和猕猴藏酋猴及熊猴的采精及其精液特征的初步研究》", 《动物学研究》, vol. 15, no. 1, 31 December 1994 (1994-12-31), pages 77 - 83 * |
| 黎宗强等: "《食蟹猴阴茎电刺激采精及精液特征的初步研究》", 《广西农业生物科学》, vol. 23, no. 3, 30 September 2004 (2004-09-30), pages 217 - 221 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758082A (en) * | 2015-03-06 | 2015-07-08 | 华南农业大学 | Semen collection method of macaque |
| CN107669370A (en) * | 2016-08-02 | 2018-02-09 | 西南大学 | The optimization method of one elite stand musk deer electro-ejaculation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103784214B (en) | 2016-05-25 |
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Address after: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant after: Suzhou medicine bright Kant's new drug development limited company Applicant after: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. Address before: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant before: Suzhou AppTec Co., Ltd. Applicant before: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: SUZHOU APPTEC CO., LTD. TO: SUZHOU YAOMING KANGDE NEW DRUG DEVELOPMENT CO., LTD. |
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Effective date of registration: 20160412 Address after: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant after: Suzhou medicine bright Kant's new drug development limited company Address before: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant before: Suzhou medicine bright Kant's new drug development limited company Applicant before: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. |
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Address after: 215104 No. 1318 Wuzhong Avenue, Wuzhong District, Suzhou City, Jiangsu Province Patentee after: Suzhou AppTec Co., Ltd. Address before: 215104 No. 1318 Wuzhong Avenue, Wuzhong District, Suzhou City, Jiangsu Province Patentee before: Suzhou medicine bright Kant's new drug development limited company |