CN103784214B - The method of monkey semen collection - Google Patents
The method of monkey semen collection Download PDFInfo
- Publication number
- CN103784214B CN103784214B CN201210422817.9A CN201210422817A CN103784214B CN 103784214 B CN103784214 B CN 103784214B CN 201210422817 A CN201210422817 A CN 201210422817A CN 103784214 B CN103784214 B CN 103784214B
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- China
- Prior art keywords
- monkey
- penis
- baoding
- seminal fluid
- gauze
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- 241000282693 Cercopithecidae Species 0.000 title claims abstract description 65
- 210000000582 semen Anatomy 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 17
- 210000003899 penis Anatomy 0.000 claims abstract description 28
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims description 2
- 230000001404 mediated effect Effects 0.000 claims description 2
- 238000005424 photoluminescence Methods 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 8
- 230000006378 damage Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Abstract
A kind of method that the invention discloses monkey semen collection, comprises step: 1) Baoding monkey; 2) to the processing of monkey penis; 3) stimulate monkey penis with egersimeter, make it produce seminal fluid; 4) collect seminal fluid. Acquisition method of the present invention, have simple to operate, damage little, be difficult for the advantage such as pollutions, be with a wide range of applications, the development of promotion medical research.
Description
Technical field
The present invention relates to a kind of method of semen collection, particularly relate to a kind of method of monkey semen collection.
Background technology
Monkey is important animal for research, and its evolution degree is the highest, is the ideal animals close to the mankind, with other animal phasesThan more similar to the mankind on physiology, be to show the unique kind of the mankind to tested material reaction. Utilize clear and mischievous person's liquid can judge that monkey isNo is sexal maturity, for some researchs provide information. Meanwhile, also can utilize compound composition, content in clear and mischievous person's liquid to carry out oneThe research of a little bioavilabilities. But because of the physiological structure of monkey, it is carried out to semen collection and have certain difficulty, application is not at present alsoA lot.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method of monkey semen collection. Adopt the method, can arrive monkey by Quick AcquisitionSeminal fluid, is beneficial to research.
For solving the problems of the technologies described above, the method for monkey semen collection of the present invention, comprises step:
1) Baoding monkey;
2) to the processing of monkey penis;
3) stimulate monkey penis with egersimeter, make it produce seminal fluid;
4) collect seminal fluid.
In Baoding of described step 1), position, Baoding comprises: the four limbs of monkey, and when Baoding, take and carve the breath state of observing monkey,Four limbs situation and the state of mind. As used monkey chair Baoding monkey, allow monkey be sitting on monkey chair, four limbs are fixed on monkey chair with gauze,Expose monkey penis, ensure the comfortable of monkey, can fully expose again the penis of monkey, meanwhile, the four limbs of monkey also can not hurtPeople.
Described step 2) in, when monkey penis is processed, the gauze parcel penis of selecting 0.9% physiological saline to soak, is beneficial to conductionAnd guarantee that penis is injury-free.
In described step 3), stimulate the step of monkey penis to be: by 2 electrode contacts of egersimeter to for wrapping up penisOn gauze, it is per second that egersimeter is set to 10 to 30 pulses, and the duration is 1 to 3 millisecond. The voltage of egersimeter canMediate with the reaction according to monkey, as mediated between 0 to 50 volt, and should be from minimum voltage, according toNeed to increase gradually, until monkey is penetrated seminal fluid.
In described step 4), the container of collection is width, the degree of depth that opening diameter the is greater than monkey penis length that is greater than monkey penisContainer, as 10mL centrifuge tube. Use this container, both ensured that seminal fluid is easy to collect and can collect completely, be unlikely to again by dirtDye.
In the collection seminal fluid of described step 4), collect at least 24 hours time intervals of seminal fluid twice.
Acquisition method of the present invention, have simple to operate, damage little, be difficult for the advantage such as pollution, be with a wide range of applications,Promote the development of medical research.
Brief description of the drawings
Below in conjunction with accompanying drawing and detailed description of the invention, the present invention is further detailed explanation:
Fig. 1 is the state diagram of monkey while being fixed on monkey chair.
Detailed description of the invention
Clear and mischievous person's liquid acquisition method of the present invention, step is as follows:
1) Baoding monkey
Check after monkey numbering (No. ID), utilize necklace that monkey is fixed on to (as shown in Figure 1) on monkey chair, use gauze fixing limbs,Make it positive in the face of picker, be sitting on monkey chair, to expose monkey penis.
When Baoding, note the elasticity in Baoding, and breath state, four limbs situation and the state of mind of moment observation monkey, guarantee monkeyCan freely breathe, four limbs degree of tightness is moderate.
2) to the processing of monkey penis
The gauze parcel penis middle part of soaking with 0.9% physiological saline, exposes monkey glans penis, simultaneously the both sides of gauze (alongPenis direction) can be by the electric shock of egersimeter folder folder.
3) egersimeter stimulates monkey penis, produces seminal fluid
Connect egersimeter (JL-C2 egersimeter, Shanghai Jia Longjiao instrument factory manufactures), open instrument, by 2 of egersimeterIndividual electrode contact is to gauze. It is per second that egersimeter is set to 10 to 30 pulses, and the duration is 1 to 3 millisecond. Electricity thornSwash the voltage of device and mediate between 0 to 50 volt, and from minimum voltage, increase gradually as required, until monkeyPenetrate seminal fluid.
4) reconcile when egersimeter, picker is this by collection container (as 10mL centrifuge tube) being enclosed within on the glans penis of penisAcquisition mode, is ready at all times to collect seminal fluid. Wherein, the individual difference that the collecting amount of this seminal fluid is looked monkey is from 0.1g to 3g and different.Meanwhile, if need to repeatedly collect, collect at least 24 hours time intervals of seminal fluid twice.
Carry out according to the method described above collecting semen, tool has the following advantages:
1) use monkey chair Baoding monkey, be beneficial to collection, monkey can not hurt people yet simultaneously;
2) by the gauze conductive electric current soaking, few to monkey injury of penis, draw materials conveniently, be beneficial to operation;
3) seminal fluid collecting flows directly in centrifuge tube, avoids waiting contacting of pollution sources with operating personnel, and be difficult for producing and pollute,Be difficult for waste;
Therefore, the present invention can be widely used in medical science, the research of toxicology etc. Wherein, need to use sexal maturity monkey for someStudy the research that maybe needs to detect compounds content in seminal fluid, the present invention is a kind of well acquisition method.
Claims (4)
1. a method for monkey semen collection, is characterized in that, comprises step:
1) Baoding monkey;
2), to the processing of monkey penis, the gauze parcel penis of selecting 0.9% physiological saline to soak, in described gauze parcel penis, adoptsThe gauze parcel penis middle part of soaking with 0.9% physiological saline, exposes monkey glans penis, and the both sides of gauze can be by electro photoluminescence simultaneouslyOn the electric shock folder folder of device;
3) stimulate monkey penis with egersimeter, make it produce seminal fluid, stimulate the step of monkey penis to be: by 2 electricity of egersimeterThe utmost point touches the gauze for wrapping up penis, and it is per second that egersimeter is set to 10 to 30 pulses, and the duration is 1 to 3Millisecond; The voltage of egersimeter is mediated between 0 to 50 volt, and from minimum voltage, increases gradually as requiredAdd, until monkey is penetrated seminal fluid;
4) collect seminal fluid, the container of collection is 10mL centrifuge tube, collect the mode of seminal fluid and be picker by the container set of collecting at the moonOn the glans penis of stem.
2. the method for claim 1, is characterized in that: described step 1) Baoding in, position, Baoding comprises: monkeyFour limbs, when Baoding, take to carve and observe the breath state of monkey, four limbs situation and the state of mind.
3. the method for claim 1, is characterized in that: described step 1) Baoding in, use monkey chair Baoding monkey,Allow monkey be sitting on monkey chair, four limbs are fixed on monkey chair with gauze, expose monkey penis.
4. the method for claim 1, is characterized in that: described step 4) collection seminal fluid in, twice collect seminal fluidAt least 24 hours time intervals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210422817.9A CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210422817.9A CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103784214A CN103784214A (en) | 2014-05-14 |
| CN103784214B true CN103784214B (en) | 2016-05-25 |
Family
ID=50660570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210422817.9A Active CN103784214B (en) | 2012-10-30 | 2012-10-30 | The method of monkey semen collection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103784214B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758082A (en) * | 2015-03-06 | 2015-07-08 | 华南农业大学 | Semen collection method of macaque |
| CN107669370A (en) * | 2016-08-02 | 2018-02-09 | 西南大学 | The optimization method of one elite stand musk deer electro-ejaculation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856276A (en) * | 2010-06-01 | 2010-10-13 | 广西大学 | Method for collecting semen from cynomolgus monkey |
| CN101869512A (en) * | 2010-06-12 | 2010-10-27 | 昆明亚灵生物科技有限公司 | Method for collecting semen of primates of macaque by electro stimulation |
-
2012
- 2012-10-30 CN CN201210422817.9A patent/CN103784214B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856276A (en) * | 2010-06-01 | 2010-10-13 | 广西大学 | Method for collecting semen from cynomolgus monkey |
| CN101869512A (en) * | 2010-06-12 | 2010-10-27 | 昆明亚灵生物科技有限公司 | Method for collecting semen of primates of macaque by electro stimulation |
Non-Patent Citations (3)
| Title |
|---|
| 《一种改进的阴茎电刺激采精法和猕猴藏酋猴及熊猴的采精及其精液特征的初步研究》;杨上川等;《动物学研究》;19941231;第15卷(第1期);77-83 * |
| 《太行山猕猴电刺激采精试验》;侯进怀等;《河南师范大学学报（自然科学版）》;19980228;第26卷(第1期);75-77 * |
| 《食蟹猴阴茎电刺激采精及精液特征的初步研究》;黎宗强等;《广西农业生物科学》;20040930;第23卷(第3期);217-221 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103784214A (en) | 2014-05-14 |
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| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant after: Suzhou medicine bright Kant's new drug development limited company Applicant after: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. Address before: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant before: Suzhou AppTec Co., Ltd. Applicant before: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: SUZHOU APPTEC CO., LTD. TO: SUZHOU YAOMING KANGDE NEW DRUG DEVELOPMENT CO., LTD. |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Effective date of registration: 20160412 Address after: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant after: Suzhou medicine bright Kant's new drug development limited company Address before: No. 1318 Wuzhong District Wuzhong road in Suzhou city of Jiangsu Province in 215104 Applicant before: Suzhou medicine bright Kant's new drug development limited company Applicant before: Shanghai Yaoming Kangde New Medicine Development Co., Ltd. |
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Address after: 215104 No. 1318 Wuzhong Avenue, Wuzhong District, Suzhou City, Jiangsu Province Patentee after: Suzhou AppTec Co., Ltd. Address before: 215104 No. 1318 Wuzhong Avenue, Wuzhong District, Suzhou City, Jiangsu Province Patentee before: Suzhou medicine bright Kant's new drug development limited company |