CN201261786Y - Animal living body muscular tissue gene electric impulse import instrument - Google Patents
Animal living body muscular tissue gene electric impulse import instrument Download PDFInfo
- Publication number
- CN201261786Y CN201261786Y CN 200820115443 CN200820115443U CN201261786Y CN 201261786 Y CN201261786 Y CN 201261786Y CN 200820115443 CN200820115443 CN 200820115443 CN 200820115443 U CN200820115443 U CN 200820115443U CN 201261786 Y CN201261786 Y CN 201261786Y
- Authority
- CN
- China
- Prior art keywords
- tissue gene
- electricimpulse
- muscle tissue
- introducing apparatus
- living animal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The utility model discloses an electrical pulse transfection apparatus for animal living body muscular tissue gene, which comprises a power supply VCC 1+ and a central processing unit IC 1. The electrical pulse transfection apparatus is characterized in that at least three needle electrodes are arranged, each needle electrode comprises an electrode a and a power supply VCC 3+. Compared with the prior art, the utility model ensures that a wide electric field is formed through program control, adjustable operating parameter and continuous discharge, thereby improving the conversion rate of DNA and expressing efficiency.
Description
Technical field:
The utility model relates to the electric pulse generator that is used for the live body quiding gene in a kind of biotechnology, particularly a kind of living animal muscle tissue gene electricimpulse introducing apparatus.
Background technology:
A crucial technical barrier is how to improve gene is imported the intracellular efficient of living animal in the research of dna vaccination and gene therapy at present.The instrument that research and development can import exogenous DNA safety in the histocyte efficiently is the key that addresses this problem.Adopt specific electric field that active somatic cell is among the pulsed electrical field, can strengthen the permeability of cytolemma significantly, thereby dna molecular can be entered in the cell in large quantities, improve the effect of dna vaccination and gene therapy.(Electroporation is that target tissue is exposed in the of short duration square wave electric pulse environment EP) to electroporation, and the inducing cell film produces of short duration reversible perforation, and during perforate membrane, many molecules (comprising medicine, plasmid) enter cell.
Electricimpulse has been used in vivo transfection DNA in skin, liver, tumour cell and the muscle tissue.Although equipment, parameter, carrier and animal that research is adopted are different, but existing result of study confirms, the intramuscular injection plasmid DNA is in conjunction with electroporation, successfully gene is imported muscle tissue, use gene electricimpulse introducing apparatus in animal muscle tissue, to import plasmid DNA, can make muscle tissue become bio-reactor, continue synthesis secretion protein for a long time and enter blood.Zooscopy confirms, adopts low field intensity (200V/cm is following), long pulse (each pulse of 20ms-50ms) square wave electroporation, has strengthened DNA transfection efficiency in the animal muscle tissue, expression amount can be improved 100 to 1000 times.This easy, effective, stable transmission method has great potential at dna vaccination and gene therapy application facet, and by the control electroporation, can realize controlling ad hoc fashion and the time that DNA expresses, and eliminates animal individual difference.
At present, along with the development of electric punching biopsy utilisation technology, the instrument of existing multiple corresponding function is sold both at home and abroad.As WJ-2005D intelligence live body gene introducing apparatus.There is following problem in like product at present: electrode is that 2 pin electrodes and positive and negative electrode are fixed.2 pin electrode effect field area are little, when biological tissue is implemented electroporation, easily cause the DNA that injects in the tissue not in the electric field action scope, influenced DNA transformation efficiency and expression efficiency.
Summary of the invention
In order to overcome the deficiency of existing vivo gene introducing apparatus, but the purpose of this utility model provides a kind of time variable control, the adjustable continuous discharge of operating parameters and forms the electric field of broad, and then improves the living animal muscle tissue gene electricimpulse introducing apparatus of DNA transformation efficiency and expression efficiency.
The purpose of this utility model is achieved through the following technical solutions: comprise power supply VCC1+, central processing unit IC1, it is characterized in that being provided with at least 3 pin electrodes, described every pin electrode all comprises electrode a and power supply VCC3+.
Above-mentioned electrode a is preferably 3 to 7 pins.
Preferably be provided with controller J in the voltage circuit of above-mentioned electrode a.
Above-mentioned living animal muscle tissue gene electricimpulse introducing apparatus preferably is provided with at least a of indicating meter, warning howler LS.
Compared with prior art, but the utility model and then improves DNA transformation efficiency and expression efficiency by time variable control, the adjustable continuous discharge of operating parameters and form the electric field of broad.
Description of drawings
Fig. 1 is the schematic circuit of the utility model embodiment.
A1～a5 is an electrode among the figure, and b1～b5 is the electrode grounding end, and D1～D4 is the luminescent crystal diode, D5 is a crystal diode, and C1～C4 is an electrical condenser, and J is a rly., JK is the control contact of relay J, and IC1～IC7 is an integrated chip, and K1～K5 is a switch, LED1～LED4 is a charactron, LS is a warning howler, and Q1～Q16 is the crystal switch pipe, and R1～R51 is a resistance, U1～U5 is an opticcoupler, and VCC1+, VCC2+, VCC3+ are direct supply.
Embodiment
With reference to accompanying drawing 1, it is the schematic circuit of the utility model embodiment, comprise power supply VCC1+, central processing unit IC1, central processing unit IC1 adopts 8051 singlechip chips, on 22～26 pin of central processing unit IC1, be connected with 5 pin electrode a1～a5, every pin electrode all comprises electrode a (1～5) and power supply VCC3+, power supply VCC3+ is 0～260V variable voltage power supply, in the voltage circuit of electrode a1～a5, be provided with switching tube Q8, Q10, Q12, Q14, Q16, be provided with by amplifirer IC2～IC5 between the control utmost point of this switching tube and the central processing unit IC1, opticcoupler U1～U5, switching tube Q7, Q9, Q11, Q13, the controller that Q15 forms, amplifirer IC2 ~ IC5 is the integrated generation sheet of SN7407, drive photoelectronic coupler U1～U5, also be provided with controller J in the voltage circuit of electrode a1～a5 simultaneously, controller J is a rly., its control contact JK is located in the voltage circuit of power supply VCC3+ of electrode a1～a5, for can in time understanding the working condition of electricimpulse introducing apparatus, on central processing unit IC1, also be provided with indicating meter, warning howler LS, indicating meter is made up of 4 units sign indicating number pipe LED1 ~ LED4.
Claims (9)
1, a kind of living animal muscle tissue gene electricimpulse introducing apparatus comprises power supply VCC1+, central processing unit IC1, it is characterized in that being provided with at least 3 pin electrodes, and described every pin electrode all comprises electrode a and power supply VCC3+.
2, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 1 is characterized in that described electrode a is 3 to 7 pins.
3, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 1 and 2 is characterized in that being provided with controller J in the voltage circuit of described electrode a.
4, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 1 and 2 is characterized in that being provided with indicating meter.
5, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 3 is characterized in that being provided with indicating meter.
6, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 1 and 2 is characterized in that being provided with warning howler LS.
7, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 3 is characterized in that being provided with warning howler LS.
8, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 4 is characterized in that being provided with warning howler LS.
9, living animal muscle tissue gene electricimpulse introducing apparatus according to claim 5 is characterized in that being provided with warning howler LS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820115443 CN201261786Y (en) | 2008-06-02 | 2008-06-02 | Animal living body muscular tissue gene electric impulse import instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820115443 CN201261786Y (en) | 2008-06-02 | 2008-06-02 | Animal living body muscular tissue gene electric impulse import instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201261786Y true CN201261786Y (en) | 2009-06-24 |
Family
ID=40808258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200820115443 Expired - Fee Related CN201261786Y (en) | 2008-06-02 | 2008-06-02 | Animal living body muscular tissue gene electric impulse import instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201261786Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106459858A (en) * | 2014-07-28 | 2017-02-22 | 内帕基因株式会社 | Electric pulse generator for electroporator, and electroporator system provided with electric pulse generator |
-
2008
- 2008-06-02 CN CN 200820115443 patent/CN201261786Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106459858A (en) * | 2014-07-28 | 2017-02-22 | 内帕基因株式会社 | Electric pulse generator for electroporator, and electroporator system provided with electric pulse generator |
| CN106459858B (en) * | 2014-07-28 | 2018-01-30 | 内帕基因株式会社 | Electroporation electric pulse generator and the electroporation device for possessing the electric pulse generator |
| US10004899B2 (en) | 2014-07-28 | 2018-06-26 | Nepa Gene Co., Ltd. | Electric pulse generator for electroporator and electroporator apparatus provided with electric pulse generator |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090624 Termination date: 20100602 |