CN1018328B - Isolated rapid reproduction method of wintersweet - Google Patents
Isolated rapid reproduction method of wintersweetInfo
- Publication number
- CN1018328B CN1018328B CN 90104477 CN90104477A CN1018328B CN 1018328 B CN1018328 B CN 1018328B CN 90104477 CN90104477 CN 90104477 CN 90104477 A CN90104477 A CN 90104477A CN 1018328 B CN1018328 B CN 1018328B
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- CN
- China
- Prior art keywords
- litre
- medium
- additional
- bud
- sulphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The present invention relates to a fast reproduction method for plum blossoms in Vitro, which uses mature mother tree axillary buds as explants and adopts the combination of self-compounded basic culture mediums and additional plant hormones. The present invention has the advantages of short time (2 to 3 years) from seedling cultivation to seedling formation to blossom, strong stress resistance of plants, long life time, good blossoming position, small volume of products, easy marketing and easy commercialization production.
Description
The present invention relates to these flowers method for quickly breeding, do not relate to plant variety.People exsomatize to plum blossom with additional plant hormone combination by a kind of minimal medium and breed fast.
In the prior art, do not see the stripped rapid breeding method of plum blossom, find no the report of pass through inventor's retrieval yet.Only have minority kinds such as Chinese rose, cuckoo to realize quick breeding in Woody flower, Prunus (PRUNUS) does not all have to realize breeding fast though plant has nearly 30 kinds to pass through obtaining regeneration plant from in-vitro culture.From growing seedlings to seedling to flowering time long (6-7), plant resistance is poor under field conditions (factors) in existing conventional plum blossom grafting, and the life-span is short, the position height of blooming, and unfavorable moulding, small product size is big, is difficult for selling.
The objective of the invention is to propose a kind of improved method for quickly breeding, having shortened plum blossom grows seedlings to seedling to flowering time, be the improved seeds of China tradition famous flower-plum blossom and the batch production production of precious wild type, and the high-grade export-oriented commodity plum blossom that forms potted plant (or potted landscape) has been opened up approach.
The invention is characterized in that adopt the autogamy medium of low inorganic salt concentration, plant hormone is combined as compound, explant adopts the elite stand axillalry bud of growing up.
In the procedure that the present invention proposes, adopt the peripheral wounded in the battle flower bud branch of the elite stand that grows up, be cut into 15-20 centimetre length plugged ear, handle, insert in the greenhouse casting bed routinely without plant hormone, treat that bud is sprouted and then adopt axillalry bud and make explant, under aseptic condition, strike off outer scale with pocket knife, the alcohol disinfecting with 70% 3 minutes, again with 0.1% mercuric chloride solution sterilization 5 minutes, inoculate with behind the aseptic water washing 4 times.With the medium for preparing with 100 milligrams of conical flask packing (every bottled 40-50 milligram), through high temperature (120-125 ℃), high pressure (1.1KG/CM
2) sterilize to cool off and then on superclean bench, inoculate.Every combination 3-5 bottle, every bottle of 5 explants.After the inoculation culture placed the quiet cultivation of culturing room.Culturing room's temperature is controlled at (22-28 ℃), uses the daylight lamp as light source, and intensity of illumination is 1000-2000LUX, illumination every day 12 hours.Just cultivate, the auximone combination of the phytocytomine of the additional high concentration of minimal medium and low concentration induces simple bud to grow up to the bud of growing thickly; Bud carries out successive transfer culture to growing thickly, the sprout tuber that then will grow thickly is transferred to successive transfer culture on minimal medium and the additional plant hormone, every bottle of culture can grow the sturdy no offspring of 4-6 strain, to no offspring root induction, continue to cultivate with the medium of autogamy and additional plant hormone, every strain grows the sturdy root of 2-6 bar from stem after 25 days, when little strain root length arrives 1-1.5 centimetre, just from medium, take out, after running water cleans, move and cut out in the basin soil, put the greenhouse and support seedling, move on to outdoor management when treating plant length again to 15-20 centimetre.
The present invention is the method for breeding fast, grows seedlings to seedling to lack (2-3) to flowering time, and plant has strong stress resistance, and the life-span is long, and the position of blooming is good, is convenient to moulding, and small product size is little, is easy to sell, and is beneficial to commercialization production.
Fig. 1: the bud of growing thickly of axillalry bud differentiation
Fig. 2: the seedling of successive transfer culture
Fig. 3: induce the seedling seedling of taking root
Fig. 4: 2 years living test tube plantlet sprays of plum blossom
Embodiment:
Adopt the medium of low inorganic salt concentration to form ammonium nitrate (NH by following composition
4NO
3) 1200-1400 mg/litre, potassium nitrate (KNO
3) 300-500 mg/litre, calcium chloride (CaCl2H
2O) 150-300 mg/litre, magnesium sulfate (MgSO
47H
2O) 40-100 mg/litre, potassium dihydrogen phosphate (KH
2PO
4) 200-400 mg/litre, ammonium dihydrogen phosphate (ADP) (NH
4H
2PO
4) 50-100 mg/litre, sulfuric acid argon iron (FeSO
47H
2O) 13.9 mg/litre, disodium ethylene diamine tetraacetate (Na
2EDTA) 18.6 mg/litre, potassium iodide (KI) 0.83 mg/litre, boric acid (H
3BO
3) 3.1 mg/litre, manganese sulphate (MnSO
4H
2O) 8 mg/litre, zinc sulphate (ZnSO
47H
2O) 8.3 mg/litre, sodium molybdate (Na
2MoO
42H
2O) 0.25 mg/litre, copper sulphate (CuSO
45H
2O) 0.25 mg/litre, cobalt chloride (COCl
26H
2O) 0.025 mg/litre, inositol 50 mg/litre, nicotinic acid 0.5 mg/litre, glycine 1 mg/litre, thiamine hydrochloride 0.5 mg/litre, hydrochloric acid adjoin plain 0.5 mg/litre of trembling, sucrose 20 mg/litre, agar 5.5 mg/litre, PH5.8, and explant is cultivated and differentiated the bud of growing thickly in 25 days on the medium of additional plant hormone BA2 mg/litre, ZE1 mg/litre, IAA0.2 mg/litre.Successive transfer culture, the sprout tuber that will grow thickly are transferred on the medium of additional plant hormone BA0.025 mg/litre, IAA0.5 mg/litre, and the interior culture of bottle grows the sturdy no offspring of 3-5 strain after 20 days.Again to no offspring root induction, no offspring is transferred on the medium of additional plant hormone NAA0.05 mg/litre, BA0.025 mg/litre, the interior little strain of bottle grows the sturdy root of 2-5 bar from basal part of stem after 20-25 days, the root length of plantlet is taken out from medium during to 1-1.5 centimetre, after running water (or clear water) cleans, directly move and cut out in the basin in the compost, put the greenhouse and support seedling, move on to outdoor management again after to 15-20 centimetre when plant is long.[move the sanction back season in spring and autumn and grow new cauline leaf after 15-20 days, adopt winter the heavy caliber flowerpot to contain the intensive sanction plantlet that moves of compost, put culturing room or greenhouse heating (15-25 ℃) and support seedling, survival rate is more than 80%].The living plant of 2-3 just manages to make do flower bud to bloom under suitable basin sanction condition.
Claims (3)
1, the stripped rapid breeding method of a kind of plum blossom is characterized in that adopting the medium that hangs down inorganic salt concentration, and plant hormone is combined as compound, and explant adopts the elite stand axillalry bud of growing up.
2, according to claim 1, it is characterized in that medium is made up of following composition: ammonium nitrate (NH
4NO
3) 1200-1400 mg/litre, potassium nitrate (KNO
3) 300-500 mg/litre, calcium chloride (CaCl2H
2O) 150-300 mg/litre, magnesium sulfate (MgSO
47H
2O) 40-100 mg/litre, potassium dihydrogen phosphate (KH
2PO
4) 200-400 mg/litre, ammonium dihydrogen phosphate (ADP) (NH
4H
2PO
4) 50-100 mg/litre, sulfuric acid argon iron (FeSO
47H
2O) 13.9 mg/litre, disodium ethylene diamine tetraacetate (Na
2EDTA) 18.6 mg/litre, potassium iodide (KI) 0.83 mg/litre, boric acid (H
3BO
3) 3.1 mg/litre, manganese sulphate (MnSO
4H
2O) 8 mg/litre, zinc sulphate (ZnSO
47H
2O) 8.3 mg/litre, sodium molybdate (Na
2MoO
42H
2O) 0.25 mg/litre, copper sulphate (CuSO
45H
2O) 0.25 mg/litre, cobalt chloride (COCl
26H
2O) 0.025 mg/litre, inositol 50 mg/litre, nicotinic acid 0.5 mg/litre, glycine 1 mg/litre, thiamine hydrochloride 0.5 mg/litre, hydrochloric acid adjoin plain 0.5 mg/litre of trembling, sucrose 20 mg/litre, agar 5.5 mg/litre, PH5.8.
3, according to claim 1, it is characterized in that axillalry bud is just cultivated on the medium of additional BA2 mg/litre, EF1 mg/litre, IAA0.2 mg/litre carries out, successive transfer culture grow thickly the additional BA0.25 mg/litre of bud, induce seedling take root additional BA0.025 milligram, BA0.025 mg/litre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90104477 CN1018328B (en) | 1990-06-15 | 1990-06-15 | Isolated rapid reproduction method of wintersweet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90104477 CN1018328B (en) | 1990-06-15 | 1990-06-15 | Isolated rapid reproduction method of wintersweet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1053345A CN1053345A (en) | 1991-07-31 |
| CN1018328B true CN1018328B (en) | 1992-09-23 |
Family
ID=4878579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90104477 Expired CN1018328B (en) | 1990-06-15 | 1990-06-15 | Isolated rapid reproduction method of wintersweet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1018328B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599060B (en) * | 2012-03-29 | 2014-03-12 | 常熟市润丰农业有限公司 | Calyx canthus tissue culture rapid propagation method |
| CN106472320A (en) * | 2016-11-29 | 2017-03-08 | 广西南岜仔科技有限公司 | A kind of inducing culture of Flos Mume calluss |
| CN106665243A (en) * | 2016-12-21 | 2017-05-17 | 阜阳市颍泉区玉寿种植专业合作社 | Cutting seedling culturing method for plum blossoms |
| CN110100731A (en) * | 2019-05-14 | 2019-08-09 | 湖南省园艺研究所 | A method of improving plum blossom tissue-culturing rapid propagation proliferation rate |
| CN114467757B (en) * | 2022-03-07 | 2022-12-27 | 中国科学院合肥物质科学研究院 | Method for reducing formation of basal callus in chimonanthus nitens stem tissue culture regeneration |
-
1990
- 1990-06-15 CN CN 90104477 patent/CN1018328B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| CN1053345A (en) | 1991-07-31 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C13 | Decision | ||
| GR02 | Examined patent application | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |