CN106472320A - A kind of inducing culture of Flos Mume calluss - Google Patents
A kind of inducing culture of Flos Mume calluss Download PDFInfo
- Publication number
- CN106472320A CN106472320A CN201611067195.7A CN201611067195A CN106472320A CN 106472320 A CN106472320 A CN 106472320A CN 201611067195 A CN201611067195 A CN 201611067195A CN 106472320 A CN106472320 A CN 106472320A
- Authority
- CN
- China
- Prior art keywords
- mother solution
- calluss
- water
- flos mume
- pure water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention belongs to field of plant tissue culture is and in particular to a kind of inducing culture of Flos Mume calluss, by weight, including following material:100 130 parts of MS culture medium, 30 parts of mother solution I20 and 10 20 parts of pure water;Described mother solution I includes the material of following mass-volume concentration:Calcium bisulfite 1 5mg/L, iso-amylene amidopurin 2 6mg/L, 4 iodobenzene fluoroacetic acid 2 6mg/L, water-solubleazone 1 4mg/L and propiconazole 1 4mg/L.The present invention can rapid induction Flos Mume Callus formation, prevent the browning phenomenon occurring in Flos Mume tissue culture procedures, improve callus induction rate and the quality of calluss, and the induction time of calluss is short, has great dissemination and is with a wide range of applications.
Description
Technical field
The invention belongs to field of plant tissue culture is and in particular to a kind of inducing culture of Flos Mume calluss.
Background technology
Flos Mume, is traditional famous flower of China, has the application history of more than 7000 and the cultivation history of more than 3000 in China,
Various in style, during introducing and planting, cultivate numerous excellent gardening new varieties, be greatly enriched the gardens of China
Landscape.Time about the fruit maturation that is pollinated only wants two or three months for the Flos Mume, the fruit development time is short, and its fruit reaches maturation
When, embryo is not yet ripe.Seed is processed through sand Tibetan, and sowing germination rate is also very low under normal operation.Callus culture is not only
It is a kind of new tool of plant fast propagation, be also plant improvement simultaneously, the desirable route of preserving seed and useful compound production, prunus mume (sieb.) sieb.et zucc.
Flower is xylophyta, and the induction of its callus is relatively difficult, brown stain, mycelia infection often and induce in tissue culture procedures
The problem of time length etc..
Publication No. CN 102550404 A, the Chinese patent of Application No. 201110422216.3 provides a kind of Flos Mume
Callus of Leaf highly effective revulsion induction method, describe in detail in this invention plum blossom blade calluss inducing culture based component and
Inducing culture process, but explant incubation often occurs the problem of brown stain, mycelia infection etc., thus affecting calluss
Quality and inductivity, this invents does not provide detailed solution.
Content of the invention
The goal of the invention of the present invention is, for not providing method detailed in prior art to solve explant incubation
The problem of brown stain, mycelia infection etc. occurring, providing one kind can solve brown stain and the problems such as mycelia infects, thus improving plum blossom blade
The quality of calluss and the method for inductivity.
For reaching above-mentioned purpose, the technical solution adopted in the present invention is:
A kind of inducing culture of Flos Mume calluss, by weight, including following material:MS culture medium 100-130
Part, mother solution I20-30 part and pure water 10-20 part;Described mother solution I includes the material of following quality-volumetric concentration:Sulfurous acid
Hydrogen calcium 1-5mg/L, iso-amylene amidopurin 2-6mg/L, 4- iodobenzene fluoroacetic acid 2-6mg/L, water-solubleazone 1-4mg/L and the third ring
Azoles 1-4mg/L.
In the present invention, further instruction, by weight, including following material:MS culture medium 110-120 part, mother
Liquid I22-28 part and pure water 12-18 part;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 2-
4mg/L, iso-amylene amidopurin 3-5mg/L, 4- iodobenzene fluoroacetic acid 3-5mg/L, water-solubleazone 2-3mg/L and propiconazole 2-
3mg/L.
In the present invention, further instruction, by weight, including following material:115 parts of MS culture medium, mother solution I25
Part and 15 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 3mg/L, iso-amylene
Amidopurin 4mg/L, 4- iodobenzene fluoroacetic acid 4mg/L, water-solubleazone 2.5mg/L and propiconazole 2.5mg/L.
In the present invention, further instruction, the compound method of described mother solution I is as follows:First weigh sulfurous by weight
Sour hydrogen calcium, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone and propiconazole;Then, by calcium bisulfite, isoamyl
Enamino purine, 4- iodobenzene fluoroacetic acid, water-solubleazone water-solubleazone and propiconazole are separately added in part pure water, fully
Stir and fully dissolve to above-mentioned substance, then let cool to room temperature, be settled to required solvent and obtain final product mother solution I.
A kind of method of the inducing culture preparing described Flos Mume calluss, comprises the following steps:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, the pH value of regulation system 2, and this is system 3;
(3) take system 3, use high pressure steam sterilization 20-30min, then take out, after standing cooled and solidified, obtain final product inducing culture
Base.
In the present invention, further instruction, the pH value of described culture medium is 5.5-6.5.
In the present invention, further instruction, described high pressure steam sterilization, sterilization pressure is 180-200KPa.
The present invention has the advantage that compared with prior art:
The present invention has and promotes Flos Mume calluss quickly to be formed, be reduced brown stain produce and mycelia infection problem, from
And improving quality and the inductivity of plum blossom blade calluss, research finds, through carrying out inducing prunus mume (sieb.) sieb.et zucc. using the culture medium of the present invention
Flower Callus of Leaf, induction time foreshortens to 6 days, and inductivity is up to 99%, and browning rate is only 1.2%, and calluss are tight,
Color is light green and transparent, presents good state.
1. browning phenomenon often occurs during induced synthesis calluss, the generation of brown stain can have to many enzymes
Inhibitory action, thus affecting growth and the differentiation of culture materials, even resulting in death, luring of calluss also often when serious
Lead that formation is slow, the problem that inductivity is low, of low quality etc., research finds, the present invention is led on the basis to culture medium MS culture medium
Upper interpolation mother solution I formula, effectively improves problem above, and wherein, iso-amylene amidopurin is a class basic element of cell division, can have
Effect promotes cell division and expansion effect, and regulates and controls its differentiation, and 4- iodobenzene fluoroacetic acid has the physiologically active of heteroauxing, with isoamyl
Enamino purine is used in mixed way can rapidly and efficiently inducing calli induction and differentiation;Water-solubleazone is penetrating agent, can carry
Dynamic iso-amylene amidopurin and 4- iodobenzene fluoroacetic acid rapid osmotic are to intracellular;It is organized in incubation, polyphenol oxidase is
Cause the enzyme that enzymatic browning is main, the activity of enzyme causing brown stain by suppression, thus reducing enzymatic browning approach, reduces brown stain
Generation, calcium bisulfite can by suppress polyphenol oxidase activity, to during tissue culture occur browning play very
Good remission effect, propiconazole efficiently solves mycelia infection and leads to the unsuccessful problem of explant callus induction.
2.MS culture medium provide explant maintain during callus induction basic growth needed for growth because
Son, and the interpolation of mother solution I plays the role of efficient facilitation and improves calluss to calli induction.
Specific embodiment
The invention provides a kind of inducing culture of Flos Mume calluss, for make the purpose of the present invention, technical scheme and
Effect is clearer, clear and definite, and the present invention is described in more detail below.It should be appreciated that specific embodiment described herein
Only in order to explain the present invention, it is not intended to limit the present invention.
Embodiment 1
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
A kind of inducing culture of Flos Mume calluss, by weight, including following material:100 parts of MS culture medium, mother
Liquid I20 part and 10 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 1mg/L, different
Amylene amidopurin 2mg/L, 4- iodobenzene fluoroacetic acid 2mg/L, water-solubleazone 1mg/L and propiconazole 1mg/L.
The compound method of mother solution I is as follows:
Weigh calcium bisulfite 1mg, iso-amylene amidopurin 2mg, 4- iodobenzene fluoroacetic acid 2mg, water-solubleazone 1mg and third
Ring azoles 1mg;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-solubleazone and
Propiconazole is separately added in pure water, is stirred well to calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-soluble
Property azone water-solubleazone and propiconazole are completely dissolved, and then let cool to room temperature, are settled to required solvent and obtain final product mother solution I.
The compound method of culture medium is as follows:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, and the pH value of regulation system 2 is 5.5, and this is system 3;
(3) take system 3, system 3 is carried out high pressure steam sterilization 20min with the sterilization pressure of 180KPa, then takes out, quiet
Inducing culture is obtained final product after putting cooled and solidified.
Embodiment 2
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
A kind of inducing culture of Flos Mume calluss it is characterised in that by weight, including following material:MS trains
115 parts of foster base, mother solution I25 part and 15 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Sulfurous acid
Hydrogen calcium 3mg/L, iso-amylene amidopurin 4mg/L, 4- iodobenzene fluoroacetic acid 4mg/L, water-solubleazone 2.5mg/L and propiconazole
2.5mg/L.
The compound method of mother solution I is as follows:
Weigh calcium bisulfite 3mg, iso-amylene amidopurin 4mg, 4- iodobenzene fluoroacetic acid 4mg, water-solubleazone 2.5mg and
Propiconazole 2.5mg;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-soluble nitrogen
Ketone and propiconazole are separately added in pure water, be stirred well to calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid,
Water-solubleazone water-solubleazone and propiconazole are completely dissolved, and then let cool to room temperature, are settled to required solvent and obtain final product mother solution I.
The compound method of culture medium is as follows:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, and the pH value of regulation system 2 is 6.0, and this is system 3;
(3) take system 3, system 3 is carried out high pressure steam sterilization 25min with the sterilization pressure of 190KPa, then takes out, quiet
Inducing culture is obtained final product after putting cooled and solidified.
Embodiment 3
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
A kind of inducing culture of Flos Mume calluss, by weight, including following material:130 parts of MS culture medium, mother
Liquid I30 part and 20 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 5mg/L, different
Amylene amidopurin 6mg/L, 4- iodobenzene fluoroacetic acid 6mg/L, water-solubleazone 4mg/L and propiconazole 4mg/L.
The compound method of mother solution I is as follows:
Weigh calcium bisulfite 5mg, iso-amylene amidopurin 6mg, 4- iodobenzene fluoroacetic acid 6mg, water-solubleazone 4mg and third
Ring azoles 4mg;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-solubleazone and
Propiconazole is separately added in pure water, is stirred well to calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-soluble
Property azone water-solubleazone and propiconazole are completely dissolved, and then let cool to room temperature, are settled to required solvent and obtain final product mother solution I.
The compound method of culture medium is as follows:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, and the pH value of regulation system 2 is 6.5, and this is system 3;
(3) take system 3, system 3 is carried out high pressure steam sterilization 30min with the sterilization pressure of 200KPa, then takes out, quiet
Inducing culture is obtained final product after putting cooled and solidified.
Embodiment 4
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
A kind of inducing culture of Flos Mume calluss, by weight, including following material:110 parts of MS culture medium, mother
Liquid I22 part and 12 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 2mg/L, different
Amylene amidopurin 3mg/L, 4- iodobenzene fluoroacetic acid 3mg/L, water-solubleazone 2mg/L and propiconazole 2mg/L.
The compound method of mother solution I is as follows:
Weigh calcium bisulfite 2mg, iso-amylene amidopurin 3mg, 4- iodobenzene fluoroacetic acid 3mg, water-solubleazone 2mg and third
Ring azoles 2mg;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-solubleazone and
Propiconazole is separately added in pure water, is stirred well to calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-soluble
Property azone water-solubleazone and propiconazole are completely dissolved, and then let cool to room temperature, are settled to required solvent and obtain final product mother solution I.
The compound method of culture medium is as follows:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, and the pH value of regulation system 2 is 5.7, and this is system 3;
(3) take system 3, system 3 is carried out high pressure steam sterilization 22min with the sterilization pressure of 185KPa, then takes out, quiet
Inducing culture is obtained final product after putting cooled and solidified.
Embodiment 5
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
A kind of inducing culture of Flos Mume calluss, by weight, including following material:120 parts of MS culture medium, mother
Liquid I28 part and 18 parts of pure water;Described mother solution I includes the material of following quality-volumetric concentration:Calcium bisulfite 4mg/L, different
Amylene amidopurin 5mg/L, 4- iodobenzene fluoroacetic acid 5mg/L, water-solubleazone 3mg/L and propiconazole 3mg/L.
The compound method of mother solution I is as follows:
Weigh calcium bisulfite 4mg, iso-amylene amidopurin 5mg, 4- iodobenzene fluoroacetic acid 5mg, water-solubleazone 3mg and third
Ring azoles 3mg;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-solubleazone and
Propiconazole is separately added in pure water, is stirred well to calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-soluble
Property azone water-solubleazone and propiconazole are completely dissolved, and then let cool to room temperature, are settled to required solvent and obtain final product mother solution I.
The compound method of culture medium is as follows:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, heating makes pure decocting in water
After boiling stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this
For system 1;
(2) weigh mother solution I by weight, mother solution I is added in system 1, be stirred continuously and make system 1 and mother solution I mixing
Uniformly, this is system 2, and the pH value of regulation system 2 is 6.3, and this is system 3;
(3) take system 3, system 3 is carried out high pressure steam sterilization 28min with the sterilization pressure of 195KPa, then takes out, quiet
Inducing culture is obtained final product after putting cooled and solidified.
Embodiment 6
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
Wherein, described mother solution I is by the material composition of following quality-volumetric concentration:Iso-amylene amidopurin 4mg/L, 4- iodine
Phenoxy acetic acid 4mg/L, water-solubleazone 2.5mg/L and propiconazole 2.5mg/L, remaining is same as Example 2.
Embodiment 7
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
Wherein, described mother solution I is by the material composition of following quality-volumetric concentration:Calcium bisulfite 3mg/L, 4- iodobenzene oxygen
Acetic acid 4mg/L, water-solubleazone 2.5mg/L and propiconazole 2.5mg/L, remaining is same as Example 2.
Embodiment 8
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
Wherein, described mother solution I is by the material composition of following quality-volumetric concentration:Calcium bisulfite 3mg/L, iso-amylene ammonia
Base purine 4mg/L, water-solubleazone 2.5mg/L and propiconazole 2.5mg/L, remaining is same as Example 2.
Example example 9
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
Wherein, described mother solution I is by the material composition of following quality-volumetric concentration:Calcium bisulfite 3mg/L, iso-amylene ammonia
Base purine 4mg/L, 4- iodobenzene fluoroacetic acid 4mg/L and propiconazole 2.5mg/L, remaining is same as Example 2.
Embodiment 10
The inducing culture of Flos Mume calluss that the present invention provides and its compound method are as follows:
Wherein, described mother solution I is by the material composition of following quality-volumetric concentration:Calcium bisulfite 3mg/L, iso-amylene ammonia
Base purine 4mg/L, 4- iodobenzene fluoroacetic acid 4mg/L and water-solubleazone 2.5mg/L, remaining is same as Example 2.
Test example:
Take the tender plum blossom blade of children, remove petiole, cut into small pieces with scalpel, draw 3 knives with blade along master pulse it is ensured that leaf
It is connected at edge it is therefore intended that making blade produce more wounds, then be inoculated in corresponding culture medium with tweezers, each bottle
Inoculation 3, inoculates 30 bottles for every group, inoculates 10 groups altogether, above operation completes on super-clean bench, be placed in identical environment after sealing mouth
Middle through row culture, cultivate to producing calluss, condition of culture is:(25 ± 1) DEG C, light culture.During record Callus formation
Between, the fragmentary degree of the color of calluss, granule, count its inductivity and browning rate, result such as following table:
As seen from the above table, because embodiment 1-5 is cultivated using the culture medium of the present invention, its induction time is substantially than it
His test group is short, and inductivity is higher than other test group, and browning rate is low, and the fragmentary degree of granule and color are all substantially better than other tests
Group.
Embodiment 6-11 is comparative example, wherein:
Without calcium bisulfite in the composition of mother solution I in embodiment 6;
Without iso-amylene amidopurin in the composition of mother solution I in embodiment 7;
Without 4- iodobenzene fluoroacetic acid in the composition of mother solution I in embodiment 8;
Do not add water-solubleazone in the composition of mother solution I in embodiment 9;
Without propiconazole in the components Component of mother solution I in embodiment 10.
By the data of comparative example 6 and embodiment 2 it can be verified that the interpolation of calcium bisulfite can greatly reduce brown stain
Produce;By the data of comparative example 7, embodiment 8 and embodiment 2 it can be verified that iso-amylene amidopurin and 4- iodobenzene oxygen second
Acid is used in mixed way quickening Callus formation;By the data of comparative example 9 and embodiment 2 it can be verified that water-soluble
Property azone can accelerate iso-amylene amidopurin and the performance of 4- iodobenzene fluoroacetic acid effect;By comparative example 10 and embodiment 2
Data it can be verified that propiconazole efficiently solve mycelia infect thus improving explant callus induction rate.
Described above is the detailed description for the preferable possible embodiments of the present invention, but embodiment is not limited to this
Bright patent claim, the equal change being completed under the technical spirit suggested by all present invention or modification change, all should belong to
In the covered the scope of the claims of the present invention.
Claims (7)
1. a kind of inducing culture of Flos Mume calluss it is characterised in that by weight, including following material:MS cultivates
Base 100-130 part, mother solution I20-30 part and pure water 10-20 part;Described mother solution I includes the thing of following quality-volumetric concentration
Matter:Calcium bisulfite 1-5mg/L, iso-amylene amidopurin 2-6mg/L, 4- iodobenzene fluoroacetic acid 2-6mg/L, water-solubleazone 1-
4mg/L and propiconazole 1-4mg/L.
2. a kind of inducing culture of Flos Mume calluss according to claim 1 is it is characterised in that by weight,
Including following material:MS culture medium 110-120 part, mother solution I22-28 part and pure water 12-18 part;Described mother solution I include with
The material of lower quality-volumetric concentration:Calcium bisulfite 2-4mg/L, iso-amylene amidopurin 3-5mg/L, 4- iodobenzene fluoroacetic acid 3-
5mg/L, water-solubleazone 2-3mg/L and propiconazole 2-3mg/L.
3. a kind of inducing culture of Flos Mume calluss according to claim 1 is it is characterised in that by weight,
Including following material:115 parts of MS culture medium, mother solution I25 part and 15 parts of pure water;Described mother solution I includes following quality-volume
The material of concentration:Calcium bisulfite 3mg/L, iso-amylene amidopurin 4mg/L, 4- iodobenzene fluoroacetic acid 4mg/L, water-solubleazone
2.5mg/L and propiconazole 2.5mg/L.
4. a kind of inducing culture of the Flos Mume calluss according to claim 1-3 is it is characterised in that described mother solution I
Compound method as follows:First weigh calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water solublity by weight
Azone and propiconazole;Then, by calcium bisulfite, iso-amylene amidopurin, 4- iodobenzene fluoroacetic acid, water-solubleazone water-soluble nitrogen
Ketone and propiconazole are separately added in part pure water, are stirred well to above-mentioned substance and fully dissolve, and then let cool to room temperature, constant volume
Obtain final product mother solution I to required solvent.
5. a kind of method preparing the inducing culture of Flos Mume calluss described in claim 1-3 it is characterised in that include with
Lower step:
(1) by weight, weigh MS culture medium and pure water, first pure water is added in pot, after heating makes pure water boil
Stop heating, then by MS culture medium add pure water in, make MS culture medium dissolve, and be sufficiently stirred for, mix homogeneously, this be body
It is 1;
(2) weigh mother solution I by weight, mother solution I added in system 1, is stirred continuously and makes system 1 and mother solution I mix homogeneously,
This is system 2, the pH value of regulation system 2, and this is system 3;
(3) take system 3, use high pressure steam sterilization 20-30min, then take out, after standing cooled and solidified, obtain final product inducing culture.
6. a kind of inducing culture preparing Flos Mume calluss according to claim 5 method it is characterised in that:Institute
The pH value stating regulation system 2 is between 5.5-6.5.
7. a kind of inducing culture preparing Flos Mume calluss according to claim 5 method it is characterised in that:Institute
State high pressure steam sterilization, sterilization pressure is 180-200KPa.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611067195.7A CN106472320A (en) | 2016-11-29 | 2016-11-29 | A kind of inducing culture of Flos Mume calluss |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611067195.7A CN106472320A (en) | 2016-11-29 | 2016-11-29 | A kind of inducing culture of Flos Mume calluss |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106472320A true CN106472320A (en) | 2017-03-08 |
Family
ID=58274457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611067195.7A Pending CN106472320A (en) | 2016-11-29 | 2016-11-29 | A kind of inducing culture of Flos Mume calluss |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106472320A (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1053345A (en) * | 1990-06-15 | 1991-07-31 | 中国科学院武汉植物研究所 | Isolated rapid reproduction method of wintersweet |
CN102091295A (en) * | 2011-01-17 | 2011-06-15 | 赵献民 | Chinese medicinal composition for treating femoral head necrosis and preparation method thereof |
CN102550404A (en) * | 2011-12-16 | 2012-07-11 | 北京林业大学 | Efficient induction method of plum blossom blade callus |
CN102768131A (en) * | 2012-05-11 | 2012-11-07 | 北京林业大学 | Annual slide preparation method of plum blossom chromosome |
-
2016
- 2016-11-29 CN CN201611067195.7A patent/CN106472320A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1053345A (en) * | 1990-06-15 | 1991-07-31 | 中国科学院武汉植物研究所 | Isolated rapid reproduction method of wintersweet |
CN102091295A (en) * | 2011-01-17 | 2011-06-15 | 赵献民 | Chinese medicinal composition for treating femoral head necrosis and preparation method thereof |
CN102550404A (en) * | 2011-12-16 | 2012-07-11 | 北京林业大学 | Efficient induction method of plum blossom blade callus |
CN102768131A (en) * | 2012-05-11 | 2012-11-07 | 北京林业大学 | Annual slide preparation method of plum blossom chromosome |
Non-Patent Citations (2)
Title |
---|
张秦英: "抗寒梅花品种区域试验及离体培养的研究", 《中国优秀博硕士学位论文全文数据库(博士) 农业科技辑》 * |
石文芳等: "4个梅花品种的胚培养及愈伤组织诱导研究", 《中国农学通报》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103120126B (en) | Method for carrying out tissue cultivation propagation of anoectochilus roxburghii by intermittent submerged bioreactor | |
KR100808942B1 (en) | Composite for culture of agaric mushroom | |
CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
CN101574055A (en) | Lonicera edulis tissue culturing method | |
CN105815213A (en) | Establishing method for in-vitro regeneration system of Kiwi berry | |
CN106119131B (en) | A method of alleviating excess copper and inhibits Growth of Pleurotus Mycelium | |
CN102037856B (en) | Simple cordyceps militaris strain rejuvenation method | |
CN111248026B (en) | Quercus matsutake culture medium and application thereof | |
CN110301291A (en) | A kind of breeding method of hickory chick strain | |
CN108834904A (en) | The culture medium and breeding method of paper mulberry are cultivated for root tissue | |
CN106212286B (en) | A kind of laurustinus special culture media | |
CN107267398B (en) | Ganoderma lucidum liquid strain culture medium and ganoderma lucidum liquid strain culture method | |
CN109328870A (en) | A kind of mushroom cultivation substrate and preparation method thereof and application method | |
CN112442449B (en) | Ramaria original strain culture medium and application thereof as well as Ramaria original strain and culture method thereof | |
CN104620992B (en) | Herba Urariae Lagopodioidis orchid the induction of the lateral bud culture medium and cultural method | |
CN110100657A (en) | A kind of production method of Dictyophora rubrovalvata test tube stock | |
CN106472320A (en) | A kind of inducing culture of Flos Mume calluss | |
KR20080073388A (en) | Mass production of bulblet via somatic embryogenic cell culture in lily | |
CN106718885A (en) | A kind of culture medium of efficient rapid induction plum blossom Callus formation | |
CN105613301B (en) | In skill Chunlan thoroughly tissue culture method | |
CN111771729A (en) | Tissue culture and rapid propagation method for pitaya | |
CN111869506A (en) | Production method of black-skin termitomyces albuminosus bag | |
CN109156343A (en) | The culture medium and breeding method of paper mulberry are cultivated for blade | |
KR101599699B1 (en) | Method for manufacturing culture medium of sparassis crispa using antioxidantm, amino acid and vitamin | |
CN109729979A (en) | A method of promote Afriocan agapanthus body embryo to sprout synchronous rate |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |
|
RJ01 | Rejection of invention patent application after publication |