CN113875590A - Rapid cultivation method for clustered North American winter seedlings - Google Patents
Rapid cultivation method for clustered North American winter seedlings Download PDFInfo
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- 238000012364 cultivation method Methods 0.000 title claims abstract description 10
- 238000005520 cutting process Methods 0.000 claims abstract description 37
- 235000003338 Ilex verticillata Nutrition 0.000 claims abstract description 28
- 244000188413 Ilex verticillata Species 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 21
- 241000196324 Embryophyta Species 0.000 claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 241000209035 Ilex Species 0.000 claims abstract description 7
- 235000003332 Ilex aquifolium Nutrition 0.000 claims abstract description 6
- 235000002296 Ilex sandwicensis Nutrition 0.000 claims abstract description 6
- 235000002294 Ilex volkensiana Nutrition 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims abstract description 5
- 240000002618 Ilex opaca Species 0.000 claims abstract description 4
- 235000006823 Ilex opaca var arenicola Nutrition 0.000 claims abstract description 4
- 235000006824 Ilex opaca var opaca Nutrition 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000005286 illumination Methods 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000003337 fertilizer Substances 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 230000000844 anti-bacterial effect Effects 0.000 claims description 7
- 239000003899 bactericide agent Substances 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 230000035755 proliferation Effects 0.000 claims description 6
- 238000007598 dipping method Methods 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 239000003415 peat Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 238000010008 shearing Methods 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000000645 desinfectant Substances 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 3
- 239000012882 rooting medium Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 239000005747 Chlorothalonil Substances 0.000 claims description 2
- 240000001238 Gaultheria procumbens Species 0.000 claims description 2
- 235000007297 Gaultheria procumbens Nutrition 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims description 2
- 239000000417 fungicide Substances 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 150000001721 carbon Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 235000013399 edible fruits Nutrition 0.000 description 4
- 238000013138 pruning Methods 0.000 description 4
- 239000010408 film Substances 0.000 description 3
- 241001299553 Ilex chinensis Species 0.000 description 2
- 235000003366 Ilex purpurea Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 241000209034 Aquifoliaceae Species 0.000 description 1
- 235000003325 Ilex Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a rapid cultivation method of clustered North American winter young plants, which mainly comprises the steps of inducing and forming clustered proliferated seedlings on a WPM culture medium, then directly carrying out rooting culture on the clustered proliferated seedlings, and largely cultivating North American holly clustered seedlings with uniform growth states, wherein the North American holly clustered seedlings can be directly used as special seedling products for cutting cultivation to carry out artificial planting. The method can solve the problems of long input period, time and labor consumption, material resources and the like in the production process of the North America holly cutting and cultivating. The method can produce a large amount of ilex verticillata cluster seedlings, and solves the problem of shortage of ilex verticillata seedlings; meanwhile, compared with the conventional cultivation technology for producing the cut branches by planting single seedlings, the method saves the first year cultivation and trunk cutting link, shortens the cut branch production period by 1 year, and has important significance for improving the production level of the cut branches of the ilex verticillata and improving the cut branch production benefit.
Description
Technical Field
The invention relates to a method for breeding ilex verticillata, in particular to a method for quickly breeding clustered ilex verticillata seedlings.
Background
North American Ilex chinensis (Ilex verticillata) is a plant of Ilex of Aquifoliaceae, and after leaves fall off in winter, red fruits on the head of the branch show more joyful and gorgeous, and is a famous branch cutting tree species. The branches of the ilex verticillata are gorgeous and the bottle inserting period is as long as 2 months, the application forms are various, and the ornamental value is high. The product can be put off in the festival atmosphere of the New year, Christmas, sweet-and-sour festival, valentine's day and the like from the end of 11 months to around the New year, and has high market acceptance in China. At present, the North America holly cutting production is still in the preliminary stage, and China needs to import a large amount of branches every year.
According to market research, the price of each cut branch of ilex verticillata reaches tens of yuan, and the yield per mu per year can be calculated according to that each mu can be cut by nearly 1 ten thousand yuan per mu per year. The prior art shows that the cut cultivation of the ilex verticillata can be put into production after 3 years. Namely, the same plant is cut and reshaped again in the first year after planting; and culturing vegetative shoots in the next year, culturing fruit-bearing shoots in the third year, and pruning for sale. The process of producing the ilex verticillata cut branches by adopting the cultivation technology not only consumes a lot of manpower and material resources, but also has long output period and is inconvenient for later-stage utilization and development. Therefore, how to improve the production efficiency of the cutting cultivation by technical means becomes a key technical problem for the development of the ilex verticillata industry.
Disclosure of Invention
The invention aims to provide a rapid cultivation method of clustered North American winter seedlings aiming at the defects in the prior art. The method comprises the steps of inducing the seedlings to form clump proliferations on an MS improved culture medium, and directly carrying out rooting culture on the clump proliferations to massively culture ilex verticillata clump seedlings with uniform growth state, wherein the culture period is 60 days. The ilex verticillata cluster seedlings can be directly used as special seedling products for cutting cultivation to carry out artificial planting.
The related prior art mainly solves the problem of rapid propagation of seedlings, and the technology mainly solves the problem of long cutting and cultivation period of the ilex verticillata. Compared with the conventional cultivation method for producing the cut branches by planting single seedlings, the special clustered seedlings for the cutting cultivation, which are produced by adopting the technology disclosed by the invention, save the first-year cultivation trunk cutting link, shorten the cutting production period by 1 year and have important significance for improving the cutting production level of the ilex verticillata.
Specifically, the method for obtaining the clustered North American winter green seedling by adopting the technical scheme comprises the following steps:
(1) material selection and disinfection treatment: selecting strong and disease and insect pest-free branches of North American holly, growing for 1-2 years, removing old leaves, placing the branches in water culture at room temperature for 7 days, changing water for 1 time every day, taking newly grown tender stems as explants, then shearing the selected explants into 2-4 cm long stem sections with buds, soaking the stem sections with 75% of ethanol for 0.3-0.6 min, then disinfecting the stem sections with 0.1% of mercury bichloride for 6-10 min, and washing the stem sections with sterilized water for 4-6 times;
(2) and (3) culturing test-tube plantlets: cutting the explant subjected to sterilization treatment in the step (1) from a wound part contacting with a disinfectant on a clean bench under an aseptic condition, cutting the cut explant into about 1.0cm stem sections with buds, inoculating the stem sections into a culture bottle containing an induction culture medium, placing the culture bottle in an environment with a white fluorescent lamp as a light source, controlling the illumination intensity to be 1800-2200 Lux, and setting the illumination time to be 14-18 h.d.d-1Culturing at 25 +/-1 ℃ for 30 days to grow into 6-8 cm sterile test-tube seedlings;
(3) and (3) cluster propagation culture: cutting the test-tube plantlet grown in the step (2) into stem sections with 1.0-2.0 cm of length and 1-2 axillary buds, transferring the stem sections into a multiplication culture medium for multiplication culture, culturing the stem sections under a white fluorescent lamp light source, controlling the illumination intensity to be 1800-2200 Lux, and setting the illumination time to be 14-18 h.d-1Subculturing for 1 time every 35d at the temperature of 25 +/-1 ℃; robust clump propagation seedlings can be obtained in the culture medium at the same time, and the number of branches of the propagation seedlings reaches more than 4;
(4) and (3) selecting the clustered seedlings in the bottle: selecting cluster propagation seedlings with good growth vigor, and hardening and domesticating the seedlings with the height of 8-10 cm;
(5) hardening seedlings: firstly, placing ilex verticillata tissue culture seedlings in a seedling hardening chamber, wherein the seedling hardening chamber is mainly used for scattering light, the illumination intensity is 3000-4000 Lux, the temperature is controlled at 15-25 ℃, after 5-7 days of culture, opening a bottle cap, and continuing to culture for 2-3 days in the seedling hardening chamber;
(6) tissue culture seedling out-of-bottle cuttage: : taking out the whole cluster propagation seedling from the bottle, cleaning the culture medium adhered to the base of the plant, removing the yellow damaged leaves at the bottom, cutting off the branches with poor development or no elongation of the cluster propagation seedling, and only keeping 4 or 5 branches; cutting off a wound on the base part of the trimmed clustered seedlings by using a knife, dipping rooting water before cuttage, and inserting the base part into a rooting medium, wherein the cuttage medium is peat; water is sprayed at intervals in the cutting process to ensure that the tissue culture seedlings are not withered;
(7) managing after cuttage: watering thoroughly after cutting, covering a film on the seedlings, culturing for 1-2 weeks, removing the film, watering regularly, applying a foliar fertilizer, spraying a fungicide, growing a large number of roots on the base of the seedlings within 15-20 days, and obtaining robust ilex verticillata clump seedling plants after 25 days; after the cultivation is finished, the cluster seedlings are directly transplanted into a nutrition pot for planting, and the North America holly cluster seedling product is obtained.
Preferably, the induction medium in step (2) is: WPM +0.2 mg. L-1NAA+2.0mg·L-16-BA + active carbon 0.5 g.L-1，pH5.8。
Preferably, the proliferation medium in step (3) is: WPM +2.5 mg. L-1BA+0.2mg·L- 1NAA+0.5g·L-1Activated carbon, pH 5.7.
Preferably, in the step (6), the rooting water adopts ABT rooting powder No. 1, the concentration is 800-1000 ppm, the dipping time is 3-5 seconds, and the base part of the tissue culture seedling is soaked by 1-2 cm.
Further, the preparation method of each liter of rooting aqueous solution comprises the following steps: dissolving 500mg ABT rooting powder No. 1 in a small amount of ethanol, and metering to 1L with clear water.
Preferably, the plastic seedling raising tray is selected as the cuttage container in the step (6), and the matrix occupies the volume of the seedling raising tray 3/4.
Further, the peat in the step (6) is treated and sterilized by 800 times of carbendazim; 800 times of carbendazim is as follows: 1g of carbendazim with the concentration of 50% is diluted by 800mL of water.
Further, the culture temperature in the step (7) is controlled to be 20-25 ℃, the humidity is kept above 85%, the light intensity is 2000-2500 Lux, and the illumination time is 16 h/d.
Further, in the step (7), the foliar fertilizer is a mixture of the flower and the bamborl, and the bactericide is 0.1% of carbendazim and chlorothalonil.
Wherein, watering, applying foliar fertilizer and spraying bactericide at regular intervals, namely watering once every three days, spraying foliar fertilizer once every two weeks and spraying bactericide once every month.
The method can be used for large-scale production of ilex verticillata seedlings, not only meets the requirement of ilex verticillata seedlings in the market, but also solves the problem of pruning and shaping of ilex verticillata, obviously shortens the time of pruning and cultivating, and improves the benefit of pruning production.
Has the advantages that: compared with the prior art, the invention has the following advantages:
the invention provides a referable technology and method for quickly cultivating ilex verticillata cluster seedlings, and has the following advantages:
1. compared with the conventional cultivation method for producing the cut branches by planting single seedlings, the method saves the first-year cultivation trunk cutting link, shortens the cut branch production period by 1 year, and has important significance for improving the cut branch production level of the ilex verticillata.
2. The invention provides a cultivation method combining tissue culture seedling cultivation and in-bottle molding, which can quickly form cluster seedlings, and meanwhile, can produce a large amount of North America holly cluster seedlings by carrying out subculture propagation on the cluster seedlings, thereby solving the problem of shortage of the North America holly seedlings.
Detailed Description
The above-mentioned contents of the present invention are further described in detail by way of examples below, but it should not be understood that the scope of the above-mentioned subject matter of the present invention is limited to the following examples, and any technique realized based on the above-mentioned contents of the present invention falls within the scope of the present invention.
A rapid cultivation method of clustered North American winter young plants is carried out according to the following steps:
(1) material selection and disinfection treatment: selecting strong and disease and insect pest-free branches of North American ilex chinensis of 1-2 years, removing old leaves, putting the branches in water culture at room temperature for 7 days, changing water for 1 time every day, taking newly grown tender stems as explants, then shearing the selected explants into 2-4 cm long stem sections with buds, soaking the stem sections with 75% of ethanol for 0.5min, then disinfecting the stem sections with 0.1% of mercury bichloride for 8min, and washing the stem sections with sterilized water for 5 times;
(2) and (3) culturing test-tube plantlets: cutting the explant subjected to sterilization treatment in the step (1) from a wound part contacting with a disinfectant on a clean bench under an aseptic condition, cutting into about 1.0cm stem segments with buds, inoculating the stem segments into a culture bottle containing an induction culture medium, placing the culture bottle in an environment with a white fluorescent lamp as a light source, controlling the illumination intensity to be 2000Lux, and setting the illumination time to be 16 h.d-1Culturing at 25 +/-1 ℃ for 30 days to grow sterile test-tube plantlets of 6-8 cm;
(3) and (3) cluster propagation culture: cutting the test-tube plantlet grown in the step (2) into stem sections with 1.0-2.0 cm of length and 1-2 axillary buds, transferring the stem sections into a multiplication culture medium for multiplication culture, culturing the stem sections under a white fluorescent lamp light source, controlling the illumination intensity to be 2000Lux, and setting the illumination time to be 16 h.d-1Subculturing at 25 + -1 deg.C for 1 time every 35 d; in the culture medium, strong fasciculate propagation seedlings can be obtained at the same time, and the average number of branches reaches 4.8.
(4) And (3) selecting the clustered seedlings in the bottle: selecting cluster propagation seedlings with good growth vigor, and hardening and domesticating the seedlings with the height of 8-10 cm;
(5) hardening seedlings: firstly, placing ilex verticillata tissue culture seedlings in a seedling hardening chamber, wherein the seedling hardening chamber is mainly used for scattering light, the illumination intensity is 3000-4000 Lux, the temperature is controlled at 15-25 ℃, after 5 days of culture, opening a bottle cap, and continuously culturing for 3 days in the seedling hardening chamber;
(6) tissue culture seedling out-of-bottle cuttage: : taking out the whole cluster propagation seedling from the bottle, cleaning the culture medium adhered to the base of the plant, removing the yellow damaged leaves at the bottom, cutting off the branches with poor development or no elongation of the cluster propagation seedling, and only keeping 4 branches; cutting off a wound on the base part of the trimmed clustered seedlings by using a knife, dipping rooting water before cuttage, and inserting the base part into a rooting medium, wherein the cuttage medium is peat; water is sprayed at intervals in the cutting process to ensure that the tissue culture seedlings are not withered;
(7) managing after cuttage: and (3) watering thoroughly after the cuttage is finished, covering a thin film on the seedlings, culturing for 1-2 weeks, removing the film, watering regularly, applying a foliar fertilizer and a bactericide, growing a large number of root systems on the base of the seedlings within 15-20 d, wherein the rooting rate is 100%, and obtaining the ilex verticillata cluster seedling plant which has a developed root system, strong growth (the plant height is 15cm) and 4 branches after 30 d. The ilex verticillata clump seedlings are directly used as special seedling products for cutting cultivation to be artificially planted, 4 strong vegetative branches are cultivated in the 1 st year, fruit bearing branches can be cultivated in the 2 nd year, the fruit bearing branches are cut for sale, and the first cutting time can be advanced by one year.
The problem of long cutting and cultivating period of the ilex verticillata can be obviously improved through the embodiment, and compared with a conventional cultivating method for producing the cut branches by planting single seedlings, the method saves a first-year cultivating and stem cutting link and shortens the cutting and cultivating period by one year.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention in any way, and any person skilled in the art can make any simple modification, equivalent replacement, and improvement on the above embodiment without departing from the technical spirit of the present invention, and still fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. A rapid cultivation method of clustered North American winter young plants is characterized by comprising the following steps:
(1) material selection and disinfection treatment: selecting strong and disease and insect pest-free branches of North American holly, growing for 1-2 years, removing old leaves, placing the branches in water culture at room temperature for 7 days, changing water for 1 time every day, taking newly grown tender stems as explants, then shearing the selected explants into 2-4 cm long stem sections with buds, soaking the stem sections with 75% of ethanol for 0.3-0.6 min, then disinfecting the stem sections with 0.1% of mercury bichloride for 6-10 min, and washing the stem sections with sterilized water for 4-6 times;
(2) and (3) culturing test-tube plantlets: cutting the explant subjected to sterilization treatment in the step (1) from a wound part contacting with a disinfectant on a clean bench under an aseptic condition, cutting the cut explant into about 1.0cm stem sections with buds, inoculating the stem sections into a culture bottle containing an induction culture medium, placing the culture bottle in an environment with a white fluorescent lamp as a light source, controlling the illumination intensity to be 1800-2200 Lux, and setting the illumination time to be 14-18 h.d.d-1Culturing at 25 +/-1 ℃ for 30 days to grow into 6-8 cm sterile test-tube seedlings;
(3) and (3) cluster propagation culture: and (3) shearing the test-tube plantlets grown in the step (2) into 1 in length.Stem segments with 1-2 axillary buds and 0-2.0 cm are transferred into a proliferation culture medium for proliferation culture, the stem segments are cultured under a white fluorescent lamp light source, the illumination intensity is controlled to be 1800-2200 Lux, and the illumination time is set to be 14-18 h.d-1Subculturing for 1 time every 35d at the temperature of 25 +/-1 ℃; robust clump propagation seedlings can be obtained in the culture medium at the same time, and the number of branches of the propagation seedlings reaches more than 4;
(4) and (3) selecting the clustered seedlings in the bottle: selecting cluster propagation seedlings with good growth vigor, and hardening and domesticating the seedlings with the height of 8-10 cm;
(5) hardening seedlings: firstly, placing ilex verticillata tissue culture seedlings in a seedling hardening chamber, wherein the seedling hardening chamber is mainly used for scattering light, the illumination intensity is 3000-4000 Lux, the temperature is controlled at 15-25 ℃, after 5-7 days of culture, opening a bottle cap, and continuing to culture for 2-3 days in the seedling hardening chamber;
(6) tissue culture seedling out-of-bottle cuttage: taking out the whole cluster propagation seedling from the bottle, cleaning the culture medium adhered to the base of the plant, removing the yellow damaged leaves at the bottom, cutting off the branches with poor development or no elongation of the cluster propagation seedling, and only keeping 4 or 5 branches; cutting off a wound on the base part of the trimmed clustered seedlings by using a knife, dipping rooting water before cuttage, and inserting the base part into a rooting medium, wherein the cuttage medium is peat; water is sprayed at intervals in the cutting process to ensure that the tissue culture seedlings are not withered;
(7) managing after cuttage: watering thoroughly after cutting, covering a film on the seedlings, culturing for 1-2 weeks, removing the film, watering regularly, applying a foliar fertilizer, spraying a fungicide, growing a large number of roots on the base of the seedlings within 15-20 days, and obtaining robust ilex verticillata clump seedling plants after 25 days; after the cultivation is finished, the cluster seedlings are directly transplanted into a nutrition pot for planting, and the North America holly cluster seedling product is obtained.
2. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: the induction culture medium in the step (2) is as follows: WPM +0.2 mg. L-1 NAA+2.0mg·L-16-BA + active carbon 0.5 g.L-1，pH5.8。
3. The rosette of claim 1The rapid cultivation method of the North America winter green seedlings is characterized in that: the proliferation culture medium in the step (3) is as follows: WPM +2.5 mg. L-1BA+0.2mg·L-1NAA+0.5g·L-1Activated carbon, pH 5.7.
4. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: and (3) adopting ABT rooting powder No. 1 as rooting water in the step (6), wherein the concentration is 800-1000 ppm, the dipping time is 3-5 seconds, and the base part of the tissue culture seedling is soaked by 1-2 cm.
5. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: the preparation method of each liter of rooting aqueous solution comprises the following steps: dissolving 500mg ABT rooting powder No. 1 in a small amount of ethanol, and metering to 1L with clear water.
6. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: and (4) selecting a plastic seedling raising plate as the cuttage container in the step (6), wherein the matrix occupies the volume of the seedling raising plate 3/4.
7. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: treating the peat obtained in the step (6) with 800 times of carbendazim for disinfection; 800 times of carbendazim is as follows: 1g of carbendazim with the concentration of 50% is diluted by 800mL of water.
8. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: the culture temperature in the step (7) is controlled to be 20-25 ℃, the humidity is kept above 85%, the light intensity is 2000-2500 Lux, and the illumination time is 16 h/d.
9. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: in the step (7), the foliar fertilizer is a mixture of flowers and bambol which are used alternately, and the bactericide is 0.1 percent of carbendazim and chlorothalonil.
10. The method for rapidly cultivating the tufted North American winter young plant according to claim 1, wherein: watering regularly, applying foliar fertilizer and spraying bactericide, namely watering once every three days, spraying foliar fertilizer once every two weeks and spraying bactericide once every month.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117099686A (en) * | 2023-10-25 | 2023-11-24 | 西南林业大学 | North American ilex tissue culture rapid propagation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563745A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Tissue culture method of ilex verticillata |
| CN110786157A (en) * | 2018-11-23 | 2020-02-14 | 江苏省林业科学研究院 | Method for promoting rooting of ilex verticillata tissue culture seedlings |
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2021
- 2021-10-20 CN CN202111221659.6A patent/CN113875590A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103563745A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Tissue culture method of ilex verticillata |
| CN110786157A (en) * | 2018-11-23 | 2020-02-14 | 江苏省林业科学研究院 | Method for promoting rooting of ilex verticillata tissue culture seedlings |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117099686A (en) * | 2023-10-25 | 2023-11-24 | 西南林业大学 | North American ilex tissue culture rapid propagation method and application |
| CN117099686B (en) * | 2023-10-25 | 2024-01-05 | 西南林业大学 | North American ilex tissue culture rapid propagation method and application |
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