CN101824098B - 快速沉淀分离肝素钠中多硫酸软骨素的方法 - Google Patents
快速沉淀分离肝素钠中多硫酸软骨素的方法 Download PDFInfo
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- CN101824098B CN101824098B CN2010101120619A CN201010112061A CN101824098B CN 101824098 B CN101824098 B CN 101824098B CN 2010101120619 A CN2010101120619 A CN 2010101120619A CN 201010112061 A CN201010112061 A CN 201010112061A CN 101824098 B CN101824098 B CN 101824098B
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- alcohol
- heparin sodium
- sodium heparin
- resin
- precipitation
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- 229920000669 heparin Polymers 0.000 title claims abstract description 76
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 28
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical class FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 title abstract 5
- 229920001287 Chondroitin sulfate Polymers 0.000 title abstract 5
- 230000001376 precipitating effect Effects 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical class CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 claims abstract description 37
- 238000001556 precipitation Methods 0.000 claims abstract description 30
- 230000003647 oxidation Effects 0.000 claims abstract description 12
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229960001008 heparin sodium Drugs 0.000 claims description 62
- 229920002567 Chondroitin Polymers 0.000 claims description 36
- 239000011347 resin Substances 0.000 claims description 19
- 229920005989 resin Polymers 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000010521 absorption reaction Methods 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 6
- 230000003068 static effect Effects 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 238000005352 clarification Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 239000012266 salt solution Substances 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000003828 vacuum filtration Methods 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- 125000003158 alcohol group Chemical group 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract description 8
- 239000003960 organic solvent Substances 0.000 abstract description 7
- 239000012535 impurity Substances 0.000 abstract description 3
- 238000000638 solvent extraction Methods 0.000 abstract description 3
- 238000005342 ion exchange Methods 0.000 abstract description 2
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- 238000003811 acetone extraction Methods 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
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- 239000000047 product Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 102000002572 Alpha-Globulins Human genes 0.000 description 1
- 108010068307 Alpha-Globulins Proteins 0.000 description 1
- 208000037157 Azotemia Diseases 0.000 description 1
- 229920000045 Dermatan sulfate Polymers 0.000 description 1
- 206010014523 Embolism and thrombosis Diseases 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229940051593 dermatan sulfate Drugs 0.000 description 1
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical group CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000005311 nuclear magnetism Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3144325B1 (en) * | 2010-09-14 | 2020-11-04 | University Of Miyazaki | High purity heparin and production method therefor |
CN101942040B (zh) * | 2010-09-16 | 2012-06-06 | 山东海科化工集团有限公司 | 肝素钠中多硫酸软骨素的去除方法 |
CN102633906A (zh) * | 2011-04-11 | 2012-08-15 | 南通天龙畜产品有限公司 | 一种粗品肝素钠生产过程中多硫酸软骨素的去除方法 |
CN103864958A (zh) * | 2013-11-24 | 2014-06-18 | 青岛九龙生物医药有限公司 | 一种制备高纯度肝素钠的方法 |
CN105440164A (zh) * | 2013-11-24 | 2016-03-30 | 青岛九龙生物医药有限公司 | 一种提高肝素钠纯度的方法 |
CN104198635B (zh) * | 2014-08-13 | 2016-09-07 | 南京健友生化制药股份有限公司 | 一种应用快速分离蛋白纯化仪检测肝素钠中多硫酸软骨素的方法 |
CN104163877B (zh) * | 2014-08-26 | 2016-05-18 | 淮安麦德森制药有限公司 | 硫酸软骨素的制备方法 |
CN104177512B (zh) * | 2014-08-26 | 2016-04-13 | 淮安麦德森制药有限公司 | 催化法生产低分子硫酸软骨素的方法 |
CN116012383B (zh) * | 2023-03-28 | 2023-06-09 | 山东鑫晟生物技术股份有限公司 | 用于硫酸软骨素生产监测的数据处理方法 |
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Address after: 223005 Hefei North Road, Huaian economic and Technological Development Zone, Jiangsu 58 Patentee after: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Address before: 223001 Hefei North Road, Huaian Economic Development Zone, Jiangsu 58 Patentee before: HUAIAN MDC CHEMICAL Co.,Ltd. |
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Denomination of invention: Separation of chondroitin polysulfate from heparin sodium by rapid precipitation Effective date of registration: 20211226 Granted publication date: 20110622 Pledgee: Huaian Qingjiang puyintai financing Company limited by guarantee Pledgor: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Registration number: Y2021320000412 |
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Date of cancellation: 20230116 Granted publication date: 20110622 Pledgee: Huaian Qingjiang puyintai financing Company limited by guarantee Pledgor: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Registration number: Y2021320000412 |
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Address after: 223005 No. 58, Hefei Road, Huai'an City, Jiangsu Province Patentee after: Jiangsu Madsen Pharmaceutical Co.,Ltd. Country or region after: China Address before: 223005 Hefei North Road, Huaian economic and Technological Development Zone, Jiangsu 58 Patentee before: HUAIAN MAIDESEN PHARMACEUTICAL Co.,Ltd. Country or region before: China |