CN101822216A - 无刺花椒植物组织培养基配制方法 - Google Patents
无刺花椒植物组织培养基配制方法 Download PDFInfo
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- CN101822216A CN101822216A CN201010011460A CN201010011460A CN101822216A CN 101822216 A CN101822216 A CN 101822216A CN 201010011460 A CN201010011460 A CN 201010011460A CN 201010011460 A CN201010011460 A CN 201010011460A CN 101822216 A CN101822216 A CN 101822216A
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- rooting
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- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 10
- 239000003104 tissue culture media Substances 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims abstract description 7
- 241000949456 Zanthoxylum Species 0.000 title claims abstract description 5
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229920001817 Agar Polymers 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 229930006000 Sucrose Natural products 0.000 claims abstract description 12
- 239000008272 agar Substances 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000006160 differential media Substances 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 230000004069 differentiation Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 abstract description 2
- 239000012882 rooting medium Substances 0.000 abstract 4
- 235000013681 dietary sucrose Nutrition 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 229960004793 sucrose Drugs 0.000 abstract 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 1
- 239000005971 1-naphthylacetic acid Substances 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 238000005303 weighing Methods 0.000 description 8
- 241001079064 Zanthoxylum schinifolium Species 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 238000012856 packing Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000010257 thawing Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- SQGYWYIDSFRISU-UHFFFAOYSA-N 6-benzyl-7h-purin-2-amine Chemical class C=12NC=NC2=NC(N)=NC=1CC1=CC=CC=C1 SQGYWYIDSFRISU-UHFFFAOYSA-N 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2010100114606A CN101822216B (zh) | 2010-01-14 | 2010-01-14 | 无刺花椒植物组织培养基配制方法 |
Applications Claiming Priority (1)
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CN2010100114606A CN101822216B (zh) | 2010-01-14 | 2010-01-14 | 无刺花椒植物组织培养基配制方法 |
Publications (2)
Publication Number | Publication Date |
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CN101822216A true CN101822216A (zh) | 2010-09-08 |
CN101822216B CN101822216B (zh) | 2012-06-27 |
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CN2010100114606A Expired - Fee Related CN101822216B (zh) | 2010-01-14 | 2010-01-14 | 无刺花椒植物组织培养基配制方法 |
Country Status (1)
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CN (1) | CN101822216B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102893863A (zh) * | 2012-09-10 | 2013-01-30 | 向华 | 一种见血飞的快速繁殖方法 |
CN108496804A (zh) * | 2018-07-05 | 2018-09-07 | 重庆文理学院 | 无刺花椒组织培养初代诱导的方法 |
CN113331055A (zh) * | 2020-03-02 | 2021-09-03 | 重庆文理学院 | 一种无刺花椒组培苗的切割方法 |
-
2010
- 2010-01-14 CN CN2010100114606A patent/CN101822216B/zh not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102893863A (zh) * | 2012-09-10 | 2013-01-30 | 向华 | 一种见血飞的快速繁殖方法 |
CN108496804A (zh) * | 2018-07-05 | 2018-09-07 | 重庆文理学院 | 无刺花椒组织培养初代诱导的方法 |
CN113331055A (zh) * | 2020-03-02 | 2021-09-03 | 重庆文理学院 | 一种无刺花椒组培苗的切割方法 |
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Publication number | Publication date |
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CN101822216B (zh) | 2012-06-27 |
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Owner name: SHANDONG XINQIU SEED TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SHANDONG OPEN TYPE PLANT MICROPROPAGATION ENGINEERING TECHNOLOGY RESEARCH CENTER Effective date: 20111116 |
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C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent for invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Youqiu Inventor after: Tan Zhikun Inventor after: Zhao Xueyan Inventor after: Wang Shuangshuang Inventor after: Ma Yanxia Inventor before: Zhang Youqiu Inventor before: Tan Zhikun Inventor before: Zhao Xueyan Inventor before: Wang Shuangshuang |
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COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG YOUQIU TAN ZHIKUN ZHAO XUEYAN WANG SHUANGSHUANG TO: ZHANG YOUQIU TAN ZHIKUN ZHAO XUEYAN WANG SHUANGSHUANG MA YANXIA |
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TA01 | Transfer of patent application right |
Effective date of registration: 20111116 Address after: 253200, Shandong province Xiajin County Development Zone Yongle Road middle (Shandong Xin autumn Seed Technology Co., Ltd. hospital) Applicant after: Shandong Xinqiu Seed Technology Co., Ltd. Address before: 253200, Shandong province Xiajin County Development Zone Yongle Road middle (Shandong Xin autumn Seed Technology Co., Ltd. hospital) Applicant before: Shandong Open Type Plant Micropropagation Engineering Technology Research Center |
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C14 | Grant of patent or utility model | ||
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120627 Termination date: 20170114 |