CN101822216A - 无刺花椒植物组织培养基配制方法 - Google Patents

无刺花椒植物组织培养基配制方法 Download PDF

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CN101822216A
CN101822216A CN201010011460A CN201010011460A CN101822216A CN 101822216 A CN101822216 A CN 101822216A CN 201010011460 A CN201010011460 A CN 201010011460A CN 201010011460 A CN201010011460 A CN 201010011460A CN 101822216 A CN101822216 A CN 101822216A
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plant tissue
litre
culture medium
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CN101822216B (zh
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张友秋
谭志坤
赵学燕
王双双
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Shandong Xinqiu Seed Technology Co., Ltd.
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SHANDONG OPEN TYPE PLANT MICROPROPAGATION ENGINEERING TECHNOLOGY RESEARCH CENTER
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Abstract

一种无刺花椒植物组织培养基配制方法,属于农业生物技术领域。该植物组织培养基配方有分化培养基及生根培养基组成,两种培养基分别配制、配套应用,分化培养基配方:基本培养基10倍的MS、6-苄氨基嘌呤、吲哚-3-丁酸、蔗糖、琼脂粉;生根培养基配方:基本培养基10倍的1/2MS、1-萘乙酸、吲哚-3-丁酸、蔗糖、琼脂粉。本发明所述的植物组织培养基配制方法获得的分化培养基和生根基使培养周期只有25天左右,增殖倍数在5.5——6.5之间,理论上一个芽一年可以繁殖上千万株苗,而且没有变异,生产出的苗子整齐度高;生根培养基配方生根率高,当接种40天后。生根率在90——93%之间,且每棵苗生根条数在4——6之间。

Description

无刺花椒植物组织培养基配制方法
技术领域
本发明属于农业生物技术领域,涉及一种植物组织培养技术。
背景状况
花椒果皮是珍贵的调味香料,种子是高档食用油的原料。目前,花椒已受到众多人的青睐,身价倍增。但由于它全树多皮刺,给育苗、栽植和采收带来了不便。在这种情况下无刺花椒就深受种植者的喜爱,无刺花椒的种植也越来越多,苗源是供不应求,苗木价格也十分高,造成这种现象的原因是在我国无刺花椒的繁殖方式以嫁接为主,这种繁殖方式速度慢,生产出的苗质量也不高;另外就是从日本进口苗。因此增加了种植者的种植成本,即使这样,苗木的生产量也不能满足种植者的需求。
发明内容
本发明的目的是提供一种无刺花椒植物组织培养基配制方法,以解决现有技术无刺花椒繁殖方式以嫁接为主,繁殖速度慢,生产出的苗质量不高;进口苗增加种植成本,不能满足种植者的需求等问题。
本发明解决其技术问题所采用的技术方案是无刺花椒的植物组织培养基配方有分化培养基及生根培养基组成,两种培养基分别配制、配套应用,分化培养基配方:基本培养基10倍的MS、6-苄氨基嘌呤1.0~2.0毫克/升、吲哚-3-丁酸0.1~0.5毫克/升、蔗糖20000~40000毫克/升、琼脂粉4500-5500毫克/升;生根培养基配方:基本培养基10倍的1/2MS、1-萘乙酸0.1~0.5毫克/升、吲哚-3-丁酸0.1~0.5毫克/升、蔗糖20000~40000毫克/升、琼脂粉4500-5500毫克/升;其制备工艺如下:
1、扩繁基配方的制备:
按上述原料配比称量琼脂粉加入装0.8升纯净水的锅中煮沸→取0.1升10倍MS母液与6-苄氨基嘌呤、吲哚-3-丁酸溶解后加入锅中→称量蔗糖加入锅中并让其融化→定容到1.0升→调整PH值为5.8→分装30瓶进行高温121-126℃,高压0.1-0.14MPa灭菌20分钟→冷却成固体→接无菌苗进行无菌培养。
2、生根基配方的制备
按上述原料配比称量琼脂粉加入装0.8升纯净水的锅中煮沸→取0.05升10倍1/2MS与1-萘乙酸、吲哚-3-丁酸溶解后加入锅中→称量蔗糖加入锅中并让其融化→定容到1.0升→调整PH值为5.8→分装300瓶进行高温121-126℃,高压0.1-0.14MPa灭菌20分钟→冷却成固体→接单株无菌苗进行无菌培养。
采用本发明的积极效果是这个分化培养基配方使培养周期只有25天左右,分化倍数在5.5---6.5之间,理论上一个芽一年可以繁殖上千万株苗,而且没有变异,生产出的苗子整齐度高;生根培养基配方生根率高,当接种40天后。生根率在90---93%之间,且每棵苗生根条数在4---6之间。
具体实施方式
以制备10升分化培养基,10升生根培养基为例,其原料配方配比为:分化配方培养基:基本培养基10倍的MS、6-苄氨基嘌呤15毫克、吲哚-3-丁酸3毫克、蔗糖300000毫克、琼脂粉50000毫克;生根配方培养基:基本培养基10倍的1/2MS、1-萘乙酸3毫克、吲哚-3-丁酸3毫克、蔗糖200000毫克、琼脂粉50000毫克;其制备流程如下:
1、扩繁基配方的制备:
按上述原料配比称量琼脂粉克加入装8升纯净水的锅中煮沸→取1.0升10倍的MS母液与6-苄氨基嘌呤、吲哚-3-丁酸溶解后加入锅中→称量蔗糖加入锅中并让其融化→定容到10升→调整PH值为5.8→分装300瓶进行高温121-126℃,高压0.1-0.14MPa灭菌20分钟→冷却成固体→接无菌苗进行无菌培养。
2、生根基配方的制备
按上述原料配比称量琼脂粉加入装8升纯净水的锅中煮沸→取0.5升10倍的1/2MS母液与1-萘乙酸、吲哚-3-丁酸溶解后加入锅中→称量蔗糖加入锅中并让其融化→定容到10升→调整PH值为5.8→分装300瓶进行高温121-126℃,高压0.1-0.14MPa灭菌20分钟→冷却成固体→接单株无菌苗进行无菌培养。

Claims (1)

1.一种无刺花椒植物组织培养基配制方法,其特征是植物组织培养基配方有分化培养基及生根培养基组成,两种培养基分别配制、配套应用,分化培养基配方:基本培养基10倍的MS、6-苄氨基嘌呤1.0~2.0毫克/升、吲哚-3-丁酸0.1~0.5毫克/升、蔗糖20000~40000毫克/升、琼脂粉4500-5500毫克/升;生根培养基配方:基本培养基10倍的1/2MS、1-萘乙酸0.1~0.5毫克/升、吲哚-3-丁酸0.1~0.5毫克/升、蔗糖20000~40000毫克/升、琼脂粉4500-5500毫克/升。
CN2010100114606A 2010-01-14 2010-01-14 无刺花椒植物组织培养基配制方法 Expired - Fee Related CN101822216B (zh)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102893863A (zh) * 2012-09-10 2013-01-30 向华 一种见血飞的快速繁殖方法
CN108496804A (zh) * 2018-07-05 2018-09-07 重庆文理学院 无刺花椒组织培养初代诱导的方法
CN113331055A (zh) * 2020-03-02 2021-09-03 重庆文理学院 一种无刺花椒组培苗的切割方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102893863A (zh) * 2012-09-10 2013-01-30 向华 一种见血飞的快速繁殖方法
CN108496804A (zh) * 2018-07-05 2018-09-07 重庆文理学院 无刺花椒组织培养初代诱导的方法
CN113331055A (zh) * 2020-03-02 2021-09-03 重庆文理学院 一种无刺花椒组培苗的切割方法

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