CN101810690B - Livestock Chinese medicinal composition for enhancing body immunity and preparation method thereof - Google Patents
Livestock Chinese medicinal composition for enhancing body immunity and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a livestock Chinese medicinal composition for enhancing body immunity and a preparation method thereof. The Chinese medicinal composition comprises the following components in percentage by weight: 20 to 60 percent of poplar flower, 20 t0 40 percent of wild jujube, 10 to 30 percent of rhizoma coptidis and 10 to 30 percent of andrographis paniculata. The livestock Chinese medicinal composition has the characteristics of scientific and reasonable formula composition, good effect of eliminating damp, resisting diarrhea and calming nerves, no toxic or side effect or medicament residue, low production cost and convenient use, and meets the requirements on safe animal medicaments and the safety of animal-derived food.
Description
Technical field
The invention belongs to the livestock and poultry prevention area, especially a kind of poultry of enhancing human body immunity power are used Chinese medicine composition and preparation method thereof.
Background technology
China is livestock and poultry cultivation big country, and the R﹠D work of treatment livestock and poultry medicine did not all stop all the time.In the face of the market trend who becomes better and better, countries in the world require also more and more higher to edible safety, along with progress of science and technology, increasing drug residue is detected and confirms health harmful, countries in the world are all in the supervision that strengthens the residual problem of medicine, this has also produced no small impact to China's livestock and poultry breeding industry, and green health ground tcm product more and more is subjected to the attention of vast veterinary drug manufacturing enterprise.In the livestock and poultry cultivation process, poultry regular meeting is because autoimmunity descends or because diseases such as dysentery, enteritis take place in the extraneous factor influence at present.These diseases have certain infectiousness mostly, and regular meeting causes the poultry feed intake to reduce, and human body is become thin, and serious even situation that cause the death of poultry large tracts of land causes very big economic loss to the raiser.
Summary of the invention
The object of the present invention is to provide a kind of poultry of enhancing human body immunity power to use Chinese medicine composition, this Chinese medicine composition composition formula is formed scientific and reasonable, has good removing dampness dysentery relieving, the effect of calming the nerves, and has no side effect and drug residue, production cost is lower, and is easy to use.
Another object of the present invention is to provide the preparation method of a kind of poultry of enhancing human body immunity power with Chinese medicine composition.
The present invention is achieved by the following technical solutions:
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and percentage by weight thereof are:
Flower of poplar 20～60%;
Semen Ziziphi Spinosae 20～40%;
Rhizoma Coptidis 10～30%;
Herba Andrographis 10～30%.
A kind of poultry of enhancing human body immunity power are used the preparation method of Chinese medicine composition, and the step of its preparation method is:
(1) takes by weighing flower of poplar according to weight proportion, decoct with water 3 times, 2 hours for the first time, the second time and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20～1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0～2.0 in the time of 80 ℃, be incubated 1 hour, left standstill 12 hours, and filtered, obtain precipitation, the water that in precipitation, adds its 6～8 times of weight, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirring makes dissolving, filter, filtrate is incubated 25～35 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 55～65 ℃, left standstill 11～13 hours, filter, obtain precipitation, precipitation is washed till pH value to 7.0 with ethanol, wave most ethanol, obtain poplar flower extract behind the concentrate drying;
(2) take by weighing Semen Ziziphi Spinosae according to weight proportion, decoct with water after the pulverizing 3 times, 2 hours for the first time, second and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20～1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0～2.0 in the time of 75～85 ℃, is incubated 1 hour, left standstill 12 hours, filter, obtain precipitation, in precipitation, add 6～8 times of water gagings, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 90～95% alcoholic solution again, stirs and makes dissolving, filter, filtrate is incubated 25～35 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 55～65 ℃, leaves standstill 10～14 hours, filter, precipitation is washed till pH value to 7.0 with ethanol, waves most ethanol, obtains Semen Ziziphi Spinosae extract behind the concentrate drying;
(3) take by weighing Rhizoma Coptidis respectively according to weight proportion, Herba Andrographis, adding water temperature after the mixing soaked 0.5 hour, decoct secondary again, each 1.5 hours, collecting decoction, filter, filtrate is concentrated into relative density under 70～80 ℃ be 1.20～1.25, slowly adds ethanol when being chilled to 40 ℃, makes to contain amount of alcohol and reach 75%, fully stir, left standstill 11～13 hours, the leaching supernatant, residual residue adds an amount of 75% ethanol, stir evenly, left standstill 10～14 hours, and filtered, obtain the residue cleaner liquid, supernatant and residue cleaner liquid are merged back recovery ethanol to there not being the alcohol flavor, obtain mixture A behind the concentrate drying;
(4) behind the abundant mix homogeneously of mixture A that the Semen Ziziphi Spinosae extract that obtains of the poplar flower extract that step (1) is obtained, step (2) and step (3) obtain, the poultry that obtain finished product enhancing human body immunity power are used Chinese medicine composition.
Advantage of the present invention and beneficial effect are:
1, effective Chinese medicinal ingredients that flower of poplar, Rhizoma Coptidis, Herba Andrographis, Semen Ziziphi Spinosae conduct are extracted from the natural Chinese medicine plant in this Chinese medicine composition, have good removing dampness dysentery relieving, the effect of calming the nerves, but enhancing human body immunity power, have no side effect, can not produce drug residue, easy to use, cost is lower, can effectively treat common dysentery enteritis of poultry and various inflammation, and the anti-stress of calming the nerves be arranged, the effect of human body immunity improving power.
2, flower of poplar, Rhizoma Coptidis, Herba Andrographis have the effect of removing dampness dysentery relieving in this Chinese medicine composition, and the Semen Ziziphi Spinosae composition has anti-inflammation, the analgesic effect of calming the nerves.
3, the present invention fills a prescription and forms scientific and reasonablely, has good removing dampness dysentery relieving, the effect of calming the nerves, and has no side effect and drug residue, and production cost is lower, and is easy to use, meets safe veterinary drug, ensures the animal-derived food safe requirement.
The specific embodiment
The present invention is described in further detail by following examples.Need to prove: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Flower of poplar in the component of the present invention, Semen Ziziphi Spinosae, Rhizoma Coptidis, Herba Andrographis all can be bought on market and obtain.
Embodiment 1
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and weight are respectively: flower of poplar 60g; Semen Ziziphi Spinosae 20g; Rhizoma Coptidis 10g; Herba Andrographis 10g.
A kind of poultry of enhancing human body immunity power are used the preparation method of Chinese medicine composition, and the step of its preparation method is:
(1) takes by weighing flower of poplar according to weight proportion, decoct with water 3 times, 2 hours for the first time, the second time and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0 in the time of 80 ℃, be incubated 1 hour, left standstill 12 hours, and filtered, obtain precipitation, the water that in precipitation, adds its 6 times of weight, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirring makes dissolving, filter, filtrate is incubated 30 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 60 ℃, left standstill 12 hours, filter, obtain precipitation, precipitation is washed till pH value to 7.0 with ethanol, wave most ethanol, obtain poplar flower extract behind the concentrate drying;
(2) take by weighing Semen Ziziphi Spinosae according to weight proportion, decoct with water after the pulverizing 3 times, 2 hours for the first time, second and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0 in the time of 80 ℃, is incubated 1 hour, left standstill 12 hours, filter, obtain precipitation, in precipitation, add 6～8 times of water gagings, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirs and makes dissolving, filter, filtrate is incubated 30 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 60 ℃, leaves standstill 12 hours, filter, precipitation is washed till pH value to 7.0 with ethanol, waves most ethanol, obtains Semen Ziziphi Spinosae extract behind the concentrate drying;
(3) take by weighing Rhizoma Coptidis, Herba Andrographis respectively according to weight proportion, adding water temperature after the mixing soaked 0.5 hour, decoct secondary again, each 1.5 hours, collecting decoction, filter, filtrate is concentrated into relative density under 70 ℃ be 1.20, slowly adds ethanol when being chilled to 40 ℃, makes to contain amount of alcohol and reach 75%, fully stir, left standstill 12 hours, the leaching supernatant, residual residue adds an amount of 75% ethanol, stir evenly, left standstill 12 hours, and filtered, obtain the residue cleaner liquid, supernatant and residue cleaner liquid are merged back recovery ethanol to there not being the alcohol flavor, obtain mixture A behind the concentrate drying;
(4) behind the abundant mix homogeneously of mixture A that the Semen Ziziphi Spinosae extract that obtains of the poplar flower extract that step (1) is obtained, step (2) and step (3) obtain, the poultry that obtain finished product enhancing human body immunity power are used Chinese medicine composition.
Embodiment 2
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and weight are respectively: flower of poplar 20g; Semen Ziziphi Spinosae 20g; Rhizoma Coptidis 30g; Herba Andrographis 30g.
A kind of poultry of enhancing human body immunity power are used the preparation method of Chinese medicine composition, and it is characterized in that: the step of preparation method is:
(1) takes by weighing flower of poplar according to weight proportion, decoct with water 3 times, 2 hours for the first time, the second time and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 2.0 in the time of 80 ℃, be incubated 1 hour, left standstill 12 hours, and filtered, obtain precipitation, the water that in precipitation, adds its 8 times of weight, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirring makes dissolving, filter, filtrate is incubated 30 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 60 ℃, left standstill 12 hours, filter, obtain precipitation, precipitation is washed till pH value to 7.0 with ethanol, wave most ethanol, obtain poplar flower extract behind the concentrate drying;
(2) take by weighing Semen Ziziphi Spinosae according to weight proportion, decoct with water after the pulverizing 3 times, 2 hours for the first time, second and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 2.0 in the time of 80 ℃, is incubated 1 hour, left standstill 12 hours, filter, obtain precipitation, in precipitation, add 8 times of water gagings, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirs and makes dissolving, filter, filtrate is incubated 30 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 60 ℃, leaves standstill 12 hours, filter, precipitation is washed till pH value to 7.0 with ethanol, waves most ethanol, obtains Semen Ziziphi Spinosae extract behind the concentrate drying;
(3) take by weighing Rhizoma Coptidis, Herba Andrographis respectively according to weight proportion, adding water temperature after the mixing soaked 0.5 hour, decoct secondary again, each 1.5 hours, collecting decoction, filter, filtrate is concentrated into relative density under 80 ℃ be 1.25, slowly adds ethanol when being chilled to 40 ℃, makes to contain amount of alcohol and reach 75%, fully stir, left standstill 12 hours, the leaching supernatant, residual residue adds an amount of 75% ethanol, stir evenly, left standstill 12 hours, and filtered, obtain the residue cleaner liquid, supernatant and residue cleaner liquid are merged back recovery ethanol to there not being the alcohol flavor, obtain mixture A behind the concentrate drying;
(4) behind the abundant mix homogeneously of mixture A that the Semen Ziziphi Spinosae extract that obtains of the poplar flower extract that step (1) is obtained, step (2) and step (3) obtain, the poultry that obtain finished product enhancing human body immunity power are used Chinese medicine composition.
Embodiment 3
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and weight are respectively: flower of poplar 20g; Semen Ziziphi Spinosae 40g; Rhizoma Coptidis 20g; Herba Andrographis 20g.
The step of its preparation method is with embodiment 1.
Embodiment 4
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and weight are respectively: flower of poplar 50g; Semen Ziziphi Spinosae 20g; Rhizoma Coptidis 20g; Herba Andrographis 10g.
The step of its preparation method is with embodiment 1.
Embodiment 5
A kind of poultry of enhancing human body immunity power are used Chinese medicine composition, and its Chinese medicinal composition and weight are respectively: flower of poplar 30g; Semen Ziziphi Spinosae 30g; Rhizoma Coptidis 20g; Herba Andrographis 20g.
The step of its preparation method is with embodiment 1.
Clinical trial
First arrival in July, 2008 in by the end of November, 2008, carried out clinical pharmacodynamics test of the present invention on 6 pig farms such as Wuqing District kind pig farm, Tianjin, Jin Bei pig farms, whole experimental session prevents administration to 3000 barren sows in pig farm altogether, 187 of multiple symptom pigs such as treatment dysentery, diarrhoea.
After the treatment group of the present invention administration, the barren sow sickness rate only is 3.2%, than not administration barren sow sickness rate reduction by 12%; After the administration 5 days, cure 176, cure rate reaches 94.12%%.With SPSS12.0 statistics software analysis, behind the administration 5d, treatment group and matched group cure rate significant difference (p＜0.05).
The characteristic of component of the present invention:
1, poplar flower extract contains Quercetin (quercetin), Quercetin 3～β～D～glycoside (quercetin～3～β～D～glucoside), myricetin 3～β～D～galactoside (myricetin～3～β～D～galacloside), luteolin～7～β～D～glycoside (luteolin～7～β～D～glucoside).According to heat-clearing and toxic substances removing is arranged; The effect of removing dampness dysentery relieving cures mainly bacillary dysentery; Enteritis.
2, Semen Ziziphi Spinosae: contain volume fatty oil and protein, and contain sterol, triterpenes, Semen Ziziphi Spinosae saponin, volume vitamin C.Calmness, hypnosis, analgesia, anticonvulsant action are arranged; Certain hypotensive effect is arranged
1. calm, syngignoscism Semen Ziziphi Spinosae decoct all behaves with composure and somnolence for the oral or lumbar injection of rat, no matter daytime or night, normal condition or coffee are because of the excitatory state that causes, Semen Ziziphi Spinosae all can show above-mentioned effect, calm index when white mice is oral is 1.95, with barbiturate performance synergism, Semen Ziziphi Spinosae continuous application 6 days, animal sleep is shoaled, persistent period shortens, and promptly produces toleration, but can disappear after 1 week of drug withdrawal.
Oral Semen Ziziphi Spinosae can make defensive type motion trained reflex number of times significantly reduce, interior inhibition diffusion, and trained reflex disappears, and suppresses the manic phenomenon that cat is caused by morphine.
Give birth to the sedation and the indistinction of Semen Ziziphi Spinosae and Semen Ziziphi Spinosae (parched), but give birth to a little less than the Semen Ziziphi Spinosae effect, fry then inefficacy after the oil cake for a long time, have and think that its abirritative effective ingredient may be relevant with oil, other has and thinks relevant with water-soluble portion.
2. analgesia, convulsion, cooling effect are injected in intraperitoneal mouse with hot plate method proof Semen Ziziphi Spinosae decoct 5 gram/kilograms analgesic activity, and no matter mice is injected or oral cooling effect is arranged all, but can not the antagonistic experiment galvanic shock.
3. influencing Semen Ziziphi Spinosae and can cause that blood pressure continue to descend, heart-block cardiovascular system.
The hypertension that rat is formed with two kidney peplos methods, before operation or operation give Semen Ziziphi Spinosae (20～30 gram/kilograms/day is freely got food) next day, significant hypotensive effect is all arranged, but rat stays outer thin skin when eating Semen Ziziphi Spinosae, do not see calm phenomenon.
4. to burn influence the Semen Ziziphi Spinosae list with or share with Fructus Schisandrae Chinensis, all can improve the survival rate of scald white mice, the prolongation time-to-live, go back the generation and the prolongation time-to-live of deferrable rat burn shock, and can alleviate the white mice partial edema of burning.
3, Rhizoma Coptidis: heat clearing and damp drying, eliminating fire and detoxication.Be used for damp and hot feeling of fullness, the vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion, the carbuncle furuncle is quenched one's thirst in toothache; External treatment eczema, eczema, auditory meatus is suppurated.Be used for conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Be used for cold and heat and tie mutually, damp and hot middle resistance, feeling of fullness vomiting.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.Be used for incoordination between the liver and stomach, the vomiting acid regurgitation.
4, Herba Andrographis: heat-clearing and toxic substances removing, removing heat from blood, detumescence, dampness.Cure mainly cold, fever, laryngopharynx swelling and pain, aphtha of the mouth and tongue, pertussis chronic cough, dysentery, the puckery pain of pyretic stranguria, carbuncle skin infection, venom.
Claims (2)
1. the poultry of an enhancing human body immunity power are used Chinese medicine composition, it is characterized in that: the crude drug and the percentage by weight thereof that prepare this Chinese medicine composition are:
Flower of poplar 20～60%;
Semen Ziziphi Spinosae 20～40%;
Rhizoma Coptidis 10～30%;
Herba Andrographis 10～30%.
2. the poultry of an enhancing human body immunity power as claimed in claim 1 are used the preparation method of Chinese medicine composition, and it is characterized in that: the step of preparation method is:
(1) takes by weighing flower of poplar according to weight proportion, decoct with water 3 times, 2 hours for the first time, the second time and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20～1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0～2.0 in the time of 80 ℃, be incubated 1 hour, left standstill 12 hours, and filtered, obtain precipitation, the water that in precipitation, adds its 6～8 times of weight, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 95% alcoholic solution again, stirring makes dissolving, filter, filtrate is incubated 25～35 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 55～65 ℃, left standstill 11～13 hours, filter, obtain precipitation, precipitation is washed till pH value to 7.0 with ethanol, wave most ethanol, obtain poplar flower extract behind the concentrate drying;
(2) take by weighing Semen Ziziphi Spinosae according to weight proportion, decoct with water after the pulverizing 3 times, 2 hours for the first time, second and each 1 hour for the third time, merge decoction liquor, filter, filtrate is concentrated into relative density to be 1.20～1.25 and to add the 2mol/L hydrochloric acid solution regulate pH value to 1.0～2.0 in the time of 75～85 ℃, is incubated 1 hour, left standstill 12 hours, filter, obtain precipitation, in precipitation, add 6～8 times of water gagings, form mixed liquor, with 40% sodium hydroxide solution adjust pH to 7.0, adding with the isopyknic concentration of mixed liquor is 90～95% alcoholic solution again, stirs and makes dissolving, filter, filtrate is incubated 25～35 minutes with 2mol/L hydrochloric acid solution adjust pH to 2.0 down at 55～65 ℃, leaves standstill 10～14 hours, filter, precipitation is washed till pH value to 7.0 with ethanol, waves most ethanol, obtains Semen Ziziphi Spinosae extract behind the concentrate drying;
(3) take by weighing Rhizoma Coptidis respectively according to weight proportion, Herba Andrographis, adding water temperature after the mixing soaked 0.5 hour, decoct secondary again, each 1.5 hours, collecting decoction, filter, filtrate is concentrated into relative density under 70～80 ℃ be 1.20～1.25, slowly adds ethanol when being chilled to 40 ℃, makes to contain amount of alcohol and reach 75%, fully stir, left standstill 11～13 hours, the leaching supernatant, residual residue adds an amount of 75% ethanol, stir evenly, left standstill 10～14 hours, and filtered, obtain the residue cleaner liquid, supernatant and residue cleaner liquid are merged back recovery ethanol to there not being the alcohol flavor, obtain mixture A behind the concentrate drying;
(4) behind the abundant mix homogeneously of mixture A that the Semen Ziziphi Spinosae extract that obtains of the poplar flower extract that step (1) is obtained, step (2) and step (3) obtain, the poultry that obtain finished product enhancing human body immunity power are used Chinese medicine composition.
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| RU2462257C1 (en) * | 2011-05-05 | 2012-09-27 | Лариса Валентиновна Колпакова | Phytomineral composition for immunity reinforcement in poultry in poultry industry and method for using it |
| CN103041326A (en) * | 2011-10-14 | 2013-04-17 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition with effect of improving digestion for livestock and poultry, and preparation method thereof |
| CN103656386A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicinal composition for livestock capable of promoting qi circulation and removing stagnation, and purging heat and bowels and preparation method of Traditional Chinese medicinal composition |
| CN103800766A (en) * | 2012-11-12 | 2014-05-21 | 天津瑞贝特科技发展有限公司 | Chinese medicinal composition for prevention and treatment of aquatic animal parasites, preparation method and application thereof |
| CN103800768A (en) * | 2012-11-13 | 2014-05-21 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicinal composition for preventing and treating coccidiosis of poultry and preparation method thereof |
| CN108261488A (en) * | 2016-12-30 | 2018-07-10 | 天津瑞贝特科技发展有限公司 | Treat Chinese medicine composition of ectoparasite disease and preparation method thereof in fish body |
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| CN101313977A (en) * | 2008-06-27 | 2008-12-03 | 雷清莲 | Intestine toxicity syndrome vaccine oral liquid synergistic agent for livestock, poultry and aquatic product, and preparation method thereof |
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