CN101805447A - Synthesis method of high-molecular polypeptide polymer - Google Patents
Synthesis method of high-molecular polypeptide polymer Download PDFInfo
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Abstract
The invention relates to a synthesis method of a high-molecular polypeptide polymer. The method is characterized by comprising the following steps of: grinding, pulverizing and mixing; and heating in a reactor to initiate a condensation reaction to obtain a high-molecular polypeptide polymer having a peptide chain structure and the weight-average molecular weight of 1,000-20,0000, wherein aspartic acid or the aspartic acid and glutamic acid are used as raw materials. The method has advanced synthesis process, and the polypeptide compound with the peptide chain structure is formed by directly using the solid phase condensation of amino acid. In the reaction process, no organic solvent and protective group are used, and three wastes and by-products are not generated, thus the method has no adverse effect on the environment. The polypeptide polymer has wide application and obvious economic benefit, not only can be used as a chelating agent in industrial water treatment, but also can be used as a plant growth regulator and a fertilizer synergist and can improve the quality of agricultural products and the output. The polypeptide polymer prepared by using the method is biodegraded, has no residues after use and can improve the fertilizer utilization rate and reduce the fertilizer loss.
Description
Technical field
The invention belongs to the synthesis technical field of high-molecular polypeptide polymer, relating in particular to aspartic acid or aspartic acid and L-glutamic acid is that the raw material condensation reaction prepares that process water is handled and the solid phase synthesis process of fertilizer synergistic usefulness high-molecular polypeptide polymer.
Background technology
High molecular weight polypeptide polymer has characteristics such as favorable biological degradability energy and chelating, is widely used in biological, the synthetic field of medicine.The synthetic method of present this family macromolecule amount polypeptide polymer has a variety of, but generally all adopts the liquid phase method synthesis technique.Provide a kind of synthetic method of polypeptide compound as CN1649892A, in organic solvent, earlier an amino acid whose end group has been protected, with another kind of amino acid reaction,, formed polypeptide compound then along with the connection chaining of different aminoacids.
CN1699404A discloses a kind of synthetic method of polypeptide compound; this method is to connect each seed amino acid on resin one by one; so amino acid whose ordering of may command; the polypeptide chain that forms is attached on the solid-state resin; be convenient to purify and separate; then polypeptide chain is separated from resin, the effect of wherein using microwave radiation is to help to remove amino acid whose protecting group.US6897289B1 provides the synthetic method of solid-phase polypeptide, but the number of polypeptide seed amino acid is less than 30, and amino the needing protection of α end carried out the synthetic of polypeptide.
Many hydroxy-acid groups are arranged on the peptide chain structure of high molecular weight polypeptide polymer, can be used for Treatment of Industrial Water medicament, oil-field flooding used additives.High molecular weight polypeptide polymer also can be used for plant nutriment regulator and fertilizer synergist simultaneously, and can play good effect, but the present synthesis technique complexity of high molecular weight polypeptide polymer, it is higher to produce composition, makes the high-molecular polypeptide polymer can't suitability for industrialized production and be applied to actual needs.
Summary of the invention
The invention provides a kind of synthetic method of high-molecular polypeptide polymer, is that the raw material condensation reaction prepares process water and handles and the fertilizer synergistic high-molecular polypeptide polymer with aspartic acid or aspartic acid and L-glutamic acid.
Technical scheme of the present invention is, with aspartic acid or aspartic acid and L-glutamic acid is raw material, through grinding mixing, cause condensation reaction at the reactor internal heating, obtain weight-average molecular weight and be 1000～200000 high-molecular polypeptide polymer with peptide chain structure.
Described weight-average molecular weight with high-molecular polypeptide polymer of peptide chain structure is 4000～12000.
A kind of synthetic method is to be raw material with the aspartic acid, be ground to 20～200 orders with ball mill or mortar, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain weight-average molecular weight be 4000～7000 the high-molecular polypeptide polymer with peptide chain structure.
Another kind of synthetic method is to be raw material with the aspartic acid, be ground to 20～200 orders with ball mill or mortar, water reconciles into the mud cake shape, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain molecular weight be 6000～9000 the high-molecular polypeptide polymer with peptide chain structure.
Add in the aspartic acid after described the grinding that to account for the aspartic acid weight ratio be 1～3% catalyzer, this catalyzer is phosphoric acid, phosphoric acid salt, vitriol, alkali-metal carbonate, the organic acid that contains C1～C5 and its esters, comprises phosphoric acid, SODIUM PHOSPHATE, MONOBASIC, potassium primary phosphate, sodium sulfate, sodium pyrosulfate, salt of wormwood, saleratus, formic acid, potassium formiate, acetate, potassium acetate or sodium acetate.
A kind of preparation method is to be raw material with aspartic acid and L-glutamic acid, be ground to 20～200 orders and mix with ball mill or mortar, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain molecular weight be 7000～10000 the high-molecular polypeptide polymer with peptide chain structure.
Another kind of preparation method is to be raw material with aspartic acid and L-glutamic acid, be ground to 20～200 orders with ball mill or mortar, water reconciles into the mud cake shape, swimming microwave irradiation or oil bath type of heating are heated to 100～250 ℃ in reactor, cause condensation reaction, reaction finishes, and to obtain molecular weight be 8000～12000 the high-molecular polypeptide polymer with peptide chain structure.
The weight ratio of described aspartic acid and L-glutamic acid is 1: 0.1～5, and the preferred weight ratio is an aspartic acid: L-glutamic acid=1: 0.1～1.
Can add in aspartic acid after described the grinding and the L-glutamic acid and account for aspartic acid and L-glutamic acid gross weight than the catalyzer that is 1～3%, this catalyzer is phosphoric acid, phosphoric acid salt, vitriol, alkali-metal carbonate, the organic acid that contains C1～C5 and its esters, comprises phosphoric acid, SODIUM PHOSPHATE, MONOBASIC, potassium primary phosphate, sodium sulfate, sodium pyrosulfate, salt of wormwood, saleratus, formic acid, potassium formiate, acetate, potassium acetate or sodium acetate.
The time of condensation reaction is 5 minutes to 24 hours in the synthetic method of above-mentioned high-molecular polypeptide polymer.
Above-mentioned synthesis technique all adopts the solid phase condensation method, can be separately raw material with the aspartic acid, also can aspartic acid and L-glutamic acid be mixed into raw material, the purpose of grinding with ball mill or mortar is in order to make feed particles that bigger specific surface be arranged, can fully contact and mix, reaction is carried out smoothly.The adding of catalyzer can be shortened the reaction times, but little to the molecular weight influence of final product.When adopting microwave heating to cause condensation reaction, reaction times is far smaller than the reaction times of oil bath heating or other type of heating, can obtain the polypeptide polymer of weight-average molecular weight about 2000 in general several minutes, the reaction times that keeps longer can access 6000 even the polypeptide polymer of higher weight-average molecular weight.Water is in harmonious proportion raw material and can improves the contact condition of stock yard, and owing to there is part material can be dissolved in the water, and the existence of this part solution makes has increased the material that one deck is similar to glue between the solid material particle, and adding entry, that this reaction is carried out is more complete.When with aspartic acid and L-glutamic acid being the synthetic polypeptide polymer of raw material, random the contracting of aspartic acid and L-glutamic acid is incorporated in random arrangement on the peptide chain, and its chelation group is more superior as the performance of water conditioner.Preferred initiation amino acid polymeric method is microwave heating and oil bath type of heating, adopts conventional high-temperature heater or baking oven also to can be used as reactor and uses.
Synthesis technique advanced person provided by the invention; industrialization is workable, and this synthesis technique directly forms the polypeptide compound of peptide chain structure with amino acid self solid phase condensation, do not use any organic solvent and protecting group in the reaction process; the no three wastes and byproduct produce, and environment is not had any detrimentally affect.With aspartic acid and L-glutamic acid is that amino acid whose arrangement is at random in the synthetic polypeptide polymer that obtains of raw material, the structure chelation group of polypeptide polymer is numerous, hydroxy-acid group on the peptide chain exists in a large number, makes the polypeptide polymer performance significantly acid, can generate salt with alkali reaction.The weight average molecular weight range of the polypeptide polymer by this synthetic method preparation is very wide, between 1000～200000, is the mixture of multiple polypeptides.This mainly is the influence that is subjected to reaction times and reaction monomers bonding state.Being widely used of this polypeptide polymer, economic benefit is obvious, not only can be used as the sequestrant in the Treatment of Industrial Water, also can be used for plant-growth regulator and fertilizer synergist and uses, and can improve quality of agricultural product and improve output.Polypeptide polymer by this synthetic method preparation uses the back noresidue, and can improve utilization rate of fertilizer by biological degradation, reduces fertilizer loss.
Embodiment
Embodiment 1
Take by weighing L-glutamic acid 147 gram, aspartic acid 266 grams, in mortar, be ground to about 60 orders and mix.White mixture is changed in the round-bottomed flask, install stirring, stirring velocity is adjusted to per minute 20 changes.Heat with silicone oil bath.Temperature is raised to 1.5 hours 150 ℃ of times spent from room temperature, and is incubated 1 hour under this temperature, in 1 hour temperature is raised to 250 ℃ then, and insulation is 1 hour under this temperature.Reaction finishes the back cooling, obtains pale brown toner powder product 352 grams.
Get 10 gram samples and add 50 milliliters in water, stir and drip the potassium hydroxide solution dissolving down, endpoint pH is 8, and the weight-average molecular weight of measuring this polypeptide polymer is 8950.(molecular weight determination adopts Ubbelohde viscometer by national standard JJG 155-79 viscometer vertification regulation, and reference material is a polyacrylic acid.)
Polypeptide polymer to embodiment 1 preparation carries out every physical and chemical index and toxicity detection, detects data and sees Table 1.
The physics and chemistry characteristics of table 1 polypeptide polymer and toxicity detect data
Embodiment 2
Take by weighing L-glutamic acid 147 gram, aspartic acid 133 grams, about grinding in ball grinder to 20 mu and mix.White mixture is changed in the Stainless Steel Disc, long 300 millimeters of dish, wide 200 millimeters.Charging tray put in the retort furnace heat.Temperature is raised to 1.5 hours 150 ℃ of times spent from room temperature, and insulation is 1 hour under this temperature, is warming up to 230 ℃ then, 1.5 hours times spent, react cooling after 2 hours, and the soft block of product brown is high molecular weight polypeptide polymer, pulverizes the back and weighs 236.8 grams.The weight-average molecular weight of measuring is 7540.
Embodiment 3
Take by weighing L-glutamic acid 73 gram, aspartic acid 133 grams, in grinding in ball grinder to 200 purpose fineness and make the two mixing.In mixture, drip water, mixture is reconciled into the mud cake shape.Install with crucible with cover, put into microwave oven and heat, sampling after 5 minutes, this polypeptide polymer is yellow fluffy block, measuring weight-average molecular weight with viscosimetry is 1825; Continue reaction and take out after 0.5 hour, this polypeptide polymer is the fluffy block of deep yellow, pulverizes the back and weighs 176.2 grams.Measuring weight-average molecular weight is 6100.
Embodiment 4
Take by weighing L-glutamic acid 73 grams, aspartic acid 133 grams and catalyzer primary ammonium phosphate 3 grams, in mortar, be ground to about 100 orders and mixing.Mixture is changed in the round-bottomed flask, install stirring, stirring velocity is adjusted to per minute 20 changes.Heat with silicone oil bath.Temperature is raised to 1.5 hours 150 ℃ of times spent from room temperature, and is incubated 1 hour under this temperature, with 1 hour temperature is raised to 220 ℃ then, and insulation is 1.5 hours under this temperature.Reaction finishes the back cooling, obtains polypeptide polymer brown powder shape product 181 grams.Measuring weight-average molecular weight is 9450.
Embodiment 5
Take by weighing aspartic acid 133 grams, in mortar, grind the back and drip water, reconcile into the mud cake shape.Install with crucible with cover, put into microwave oven and heat, take out after 24 hours, this polypeptide polymer is the fluffy block of deep yellow, pulverizes the back and weighs 112.4 grams.Measuring its weight-average molecular weight is 189600.
Embodiment 6
Take by weighing L-glutamic acid 73 grams, aspartic acid 133 grams and catalyst sulfuric acid sodium 2 grams, mixed grinding to 120 order in ball mill.In mixture, drip water, mixture is reconciled into pasty state.Install with crucible with cover, put into microwave oven and heat, take out after 0.5 hour, this polypeptide polymer is the fluffy block of deep yellow, pulverizes the back and weighs 179.8 grams.Measuring weight-average molecular weight is 7250.
Embodiment 7
Take by weighing product 100 grams in the example 4, aspartic acid 100 grams grind mixing in mortar.Mixture is changed in the round-bottomed flask, install stirring, stirring velocity is adjusted to per minute 20 changes, and heats with silicone oil bath.Temperature is raised to 1 hour 150 ℃ of time spent from room temperature, and is incubated 1 hour under this temperature, with 1 hour temperature is raised to 220 ℃ then, and insulation is 20 hours under this temperature.Reaction finishes the back cooling, obtains polypeptide polymer chocolate brown powder shape product 176 grams.Molecule measuring is decided to be 165000.
The foregoing description 1-6 synthetic polypeptide polymer is carried out the scale-inhibiting properties test, press the mensuration-precipitation of calcium carbonate method test of GB/16632-1996 water conditioner scale-inhibiting properties.With synthetic sample dissolution of sodium hydroxide in the example, the pH value is transferred to 8.0, is mixed with Scale inhibitors, adds concentration in organism weight.The scale-inhibiting properties test result sees Table 2.
Table 2 scale-inhibiting properties test result
| The experiment number | Scale inhibition addition mg/L | Scale inhibition performance % |
| Example 1 | ???????5 | ???79.3 |
| Example 2 | ???????5 | ???71.2 |
| Example 3 | ???????5 | ???73.5 |
| Example 4 | ???????5 | ???84.5 |
| Example 6 | ???????5 | ???71.4 |
| Example 1 | ??????10 | ???83.3 |
| Example 1 | ??????15 | ???85.8 |
| The experiment number | Scale inhibition addition mg/L | Scale inhibition performance % |
| Example 1 | ??????20 | ???91.6 |
This polypeptide polymer is carried out the production and the application testing of enhanced fertilizer, with fresh feed pump polypeptide polymer is pressed 160L/h and add between one section of the urea production operation and flash evaporator, the synergistic urea of producing send packing straight bag.
After determining in urea, to add the polypeptide polymer synergistic agent, under same shower nozzle condition, measure influence to finished urea granularity, intensity, before test, reach duration of test in June, 2005 the urea particle is carried out sampling analysis, the result shows, after in urea, adding the polypeptide polymer synergistic agent, the urea content of granularity＞Φ 1.70mm rises to 85.24% from 76.92%, promptly increase by 8.32%, dust content in the urea granules reduces, the urea content that is in particular in granularity＜Φ 0.85mm reduces to 0.41% from 0.75%, has promptly reduced by 0.34%.
Table 3 urea size and ultimate compression strength analysis
As can be seen from Table 3, add polypeptide polymer in urea production, the uniform particles degree improves, and dust content reduces, and has improved the efficient of production line, has significant raising for economic benefit of enterprises.
Aforementioned polypeptides polymkeric substance synergistic urea is carried out the farm crop field test, the planting experiment data of wheat are seen Table 4.The planting experiment data of summer corn are seen Table 5 and table 6.
Table 4 wheat planting testing data
| Handle | Mu spike number (ten thousand) | Grain number per spike (individual) | Spikelet number (individual) | Infertile (individual) | Spike length (cm) | Plant height (cm) | Thousand seed weight (g) | Output (kg/ mu) | Volume increase (kg/ mu) | Amount of increase in production (%) |
| This product seed soaking | ???38.1 | ???27.1 | ??13.6 | ??1.5 | ??6.6 | ??63.1 | ??42.3 | ???435.7 | ???33.9 | ????8.4 |
| The clear water seed soaking | ???34.8 | ???26.8 | ??13.0 | ??1.7 | ??6.3 | ??59.1 | ??41.6 | ???401.8 |
As can be seen from Table 4, use the polypeptide polymer seed soaking, every mu increases by 1.0 yuan of costs, improves 33.9 kilograms of wheat yields, with the polypeptide polymer synergistic urea increasing both production and income of wheat is had less investment, the positive effect that output is high.Simultaneously quality of wheat is detected, the protein content of wheat brings up to 15.13 from 14.25, and wet gluten brings up to 34.63 from 32.47, and dry gluten brings up to 11.34 from 10.37, and quality of wheat also is improved.
Table 5 summer corn yield result
| The use age fertilizer type | Mean yield (kg/ mu) | Units increased in production (kg/ mu) | Amount of increase in production (%) |
| Typhon mouth phase synergistic urea | ??????631.13 | ?????70.61 | ????12.60 |
| Typhon mouth phase common urea | ??????610.24 | ?????49.72 | ?????8.87 |
| The jointing stage synergistic urea | ??????585.28 | ?????24.76 | ?????4.42 |
| The jointing stage common urea | ??????560.52 |
From the corn yield result of table 5 as can be seen, it is the highest to use corn yield with synergistic urea in the typhon mouth phase in four kinds of treatment processs, is 631.13kg/ mu, and than minimum output volume increase 70.61kg/ mu, amount of increase in production reaches 12.60%.
Table 6 summer corn output structure and Part Traits
| The use age fertilizer type | Mu stem number (strain) | Grain number per spike (individual) | 100-grain weight (gram) | Mean yield (kg/ mu) | Lodging rate % | Empty stalk rate % | Bald sharp cm |
| Typhon mouth phase synergistic urea | ??4723 | ??543.13 | ??29.76 | ??629.17 | ??5.53 | ???14.09 | ??0.37 |
| Typhon mouth phase common urea | ??4734 | ??525.26 | ??28.35 | ??609.34 | ??7.33 | ???10.88 | ??0.70 |
| The jointing stage synergistic urea | ??4685 | ??536.51 | ??29.37 | ??583.95 | ??7.21 | ???14.76 | ??0.77 |
| The jointing stage common urea | ??4621 | ??501.17 | ??28.01 | ??558.78 | ??9.89 | ???19.39 | ??1.12 |
From table 6 maize yield structure as can be seen, summer corn uses the difference of the output that shows behind the synergistic urea of synthetic polypeptide polymer of the present invention production mainly from grain number per spike and mu stem number, that is to say, this polypeptide polymer synergistic urea can make summer corn grain number per spike and mu stem number improve, and reduce empty stalk rate, reduce bald point, thereby impel output to improve.Field observation is also found, uses the anti-adversity ability that synergistic urea can strengthen corn, as strengthening lodging tolerance, reduces empty stalk rate, reduces bald point etc.This shows that synthetic polypeptide polymer of the present invention has good water treatmenting performance and fertilizer synergistic ability.
Embodiment 8
Take by weighing L-glutamic acid 20 grams, aspartic acid 200 grams, mixed grinding to 40 purpose fineness in mortar.In mixture, drip water, mixture is reconciled into the mud cake shape.Install with crucible with cover, put into microwave oven and heat, condensation reaction was taken out after 1 hour, and this polypeptide polymer is the fluffy block of deep yellow, pulverized the back and weighed 189 grams.Measuring weight-average molecular weight is 7800.
Embodiment 9
Take by weighing aspartic acid 266 grams, with ball mill grinding to 120 purpose fineness, adding 2.66 gram saleratus catalyst mix are even.Install with crucible with cover, put into baking oven and be heated to 150 ℃, be incubated after 24 hours and take out, this polypeptide polymer is the fluffy block of deep yellow, pulverizes the back and weighs 225 grams.Measuring its weight-average molecular weight is 11300.
Embodiment 10
Take by weighing aspartic acid 266 grams,, add 6 gram phosphoric acid catalysts and mix, splash into water then and reconcile into the mud cake shape with ball mill grinding to 80 purpose fineness.Install with crucible with cover, put into the glass flask oil bath and be heated to 100 ℃, be incubated after 20 hours and take out, this polypeptide polymer is the fluffy block of lightpink, pulverizes the back and weighs 240 grams.Measuring its weight-average molecular weight is 1700.
Embodiment 11
Take by weighing L-glutamic acid 665 grams, aspartic acid 133 grams, mixed grinding to 200 purpose fineness in mortar.Add catalyzer 16 gram sodium acetates and mix, in said mixture, drip water, mixture is reconciled into the mud cake shape.Install with crucible with cover, put into microwave oven and heat, condensation reaction was taken out after 3 hours, and this polypeptide polymer is the fluffy block of deep yellow, pulverized the back and weighed 710 grams.Measuring weight-average molecular weight is 14550.
Embodiment 12
Take by weighing L-glutamic acid 665 grams, aspartic acid 133 grams, mixed grinding to 200 purpose fineness in mortar.In said mixture, drip water, mixture is reconciled into the mud cake shape.Install with crucible with cover, put into microwave oven and heat, condensation reaction was taken out after 30 minutes, and this polypeptide polymer is the fluffy block of deep yellow, pulverized the back and weighed 693 grams.Measuring weight-average molecular weight is 6450.
Embodiment 13
Take by weighing L-glutamic acid 133 gram, aspartic acid 133 grams, in grinding in ball grinder to 20 purpose fineness and make the two mixing.In mixture, add formic acid catalyzer 3 grams.Install with crucible with cover, put into 250 ℃ of heating of baking oven and carry out condensation reaction, sampling after 5 minutes, measuring weight-average molecular weight with viscosimetry is 830; Continue reaction and take out after 6 hours, this polypeptide polymer is the fluffy block of deep yellow, pulverizes the back and weighs 232 grams.Measuring weight-average molecular weight is 31200.
Claims (10)
1. the synthetic method of a high-molecular polypeptide polymer, it is characterized in that it is a raw material with aspartic acid or aspartic acid and L-glutamic acid, through grinding mixing, cause condensation reaction at the reactor internal heating, obtain weight-average molecular weight and be 1000～200000 high-molecular polypeptide polymer with peptide chain structure.
2. the synthetic method of high-molecular polypeptide polymer according to claim 1 is characterized in that described weight-average molecular weight with high-molecular polypeptide polymer of peptide chain structure is 4000～12000.
3. the synthetic method of high-molecular polypeptide polymer according to claim 1, it is characterized in that with the aspartic acid being raw material, be ground to 20～200 orders with ball mill or mortar, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain weight-average molecular weight be 4000～7000 the high-molecular polypeptide polymer with peptide chain structure.
4. the synthetic method of high-molecular polypeptide polymer according to claim 1, it is characterized in that with the aspartic acid being raw material, be ground to 20～200 orders with ball mill or mortar, water reconciles into the mud cake shape, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain molecular weight be 6000～9000 the high-molecular polypeptide polymer with peptide chain structure.
5. according to the synthetic method of claim 3 and 4 described high-molecular polypeptide polymers, it is characterized in that adding in the aspartic acid after described the grinding that to account for the aspartic acid weight ratio be 1～3% catalyzer, this catalyzer is phosphoric acid, phosphoric acid salt, vitriol, alkali-metal carbonate, the organic acid that contains C1～C5 and its esters, comprises phosphoric acid, SODIUM PHOSPHATE, MONOBASIC, potassium primary phosphate, sodium sulfate, sodium pyrosulfate, salt of wormwood, saleratus, formic acid, potassium formiate, acetate, potassium acetate or sodium acetate.
6. the synthetic method of high-molecular polypeptide polymer according to claim 1, it is characterized in that with aspartic acid and L-glutamic acid be raw material, be ground to 20～200 orders and mix with ball mill or mortar, in reactor, be heated to 100～250 ℃ with microwave irradiation or oil bath type of heating, cause condensation reaction, reaction finishes, and to obtain molecular weight be 7000～10000 the high-molecular polypeptide polymer with peptide chain structure.
7. the synthetic method of high-molecular polypeptide polymer according to claim 1, it is characterized in that with aspartic acid and L-glutamic acid be raw material, be ground to 20～200 orders with ball mill or mortar, water reconciles into the mud cake shape, swimming microwave irradiation or oil bath type of heating are heated to 100～250 ℃ in reactor, cause condensation reaction, reaction finishes, and to obtain molecular weight be 8000～12000 the high-molecular polypeptide polymer with peptide chain structure.
8. according to the synthetic method of claim 6 and 7 described high-molecular polypeptide polymers, the weight ratio that it is characterized in that aspartic acid and L-glutamic acid is 1: 0.1～5, and the preferred weight ratio is an aspartic acid: L-glutamic acid=1: 0.1～1.
9. according to the synthetic method of claim 6 and 7 described high-molecular polypeptide polymers, it is characterized in that to add in aspartic acid after described the grinding and the L-glutamic acid and account for aspartic acid and L-glutamic acid gross weight than the catalyzer that is 1～3%, this catalyzer is phosphoric acid, phosphoric acid salt, vitriol, alkali-metal carbonate, the organic acid that contains C1～C5 and its esters, comprises phosphoric acid, SODIUM PHOSPHATE, MONOBASIC, potassium primary phosphate, sodium sulfate, sodium pyrosulfate, salt of wormwood, saleratus, formic acid, potassium formiate, acetate, potassium acetate or sodium acetate.
10. according to the synthetic method of claim 3,4,6 and 7 described high-molecular polypeptide polymers, the time that it is characterized in that described condensation reaction is 5 minutes to 24 hours.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017203A (en) * | 2014-06-11 | 2014-09-03 | 南京工业大学 | Preparation method and application of special hybrid polyaspartic acid for agriculture |
| CN104355722A (en) * | 2014-09-25 | 2015-02-18 | 成都新柯力化工科技有限公司 | Polyglutamic acid-phosphate fertilizer and preparation method thereof |
| CN105746561A (en) * | 2016-03-22 | 2016-07-13 | 广东中迅农科股份有限公司 | Application technology of effective components of polypeptide pesticides in suspending agent |
| CN113331460A (en) * | 2021-07-12 | 2021-09-03 | 云南中烟工业有限责任公司 | Maillard intermediate and application thereof in tobacco flavoring |
| CN113502189A (en) * | 2021-07-12 | 2021-10-15 | 云南中烟工业有限责任公司 | Maillard product prepared from oligopeptide and application of Maillard product in tobacco flavoring |
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2010
- 2010-05-06 CN CN201010164086A patent/CN101805447A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017203A (en) * | 2014-06-11 | 2014-09-03 | 南京工业大学 | Preparation method and application of special hybrid polyaspartic acid for agriculture |
| CN104355722A (en) * | 2014-09-25 | 2015-02-18 | 成都新柯力化工科技有限公司 | Polyglutamic acid-phosphate fertilizer and preparation method thereof |
| CN105746561A (en) * | 2016-03-22 | 2016-07-13 | 广东中迅农科股份有限公司 | Application technology of effective components of polypeptide pesticides in suspending agent |
| CN113331460A (en) * | 2021-07-12 | 2021-09-03 | 云南中烟工业有限责任公司 | Maillard intermediate and application thereof in tobacco flavoring |
| CN113502189A (en) * | 2021-07-12 | 2021-10-15 | 云南中烟工业有限责任公司 | Maillard product prepared from oligopeptide and application of Maillard product in tobacco flavoring |
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Application publication date: 20100818 |