CN101805414B - Preparation method of ganoderma lucidum polysaccharide with high yield - Google Patents
Preparation method of ganoderma lucidum polysaccharide with high yield Download PDFInfo
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Abstract
The invention discloses a preparation method of ganoderma lucidum polysaccharide with high yield, which comprises the following steps: firstly, crushing ganoderma lucidum fruiting bodies into ganoderma lucidum powder, then sequentially extracting the ganoderma lucidum powder by adopting a microwave-assisted hot water extraction method and an ultrasonic-assisted hot water extraction method, and carrying out concentration, impurity removal, concentration, alcohol precipitation and drying on the obtained extracting solution to obtain water-soluble ganoderma lucidum polysaccharide; and extracting the ganoderma lucidum residues by adopting an alkali extraction method, and carrying out neutralization, dialysis, concentration, impurity removal, alcohol precipitation and drying on the obtained extracting solution to obtain alkali-soluble ganoderma lucidum polysaccharide. The method is used for extracting the ganoderma lucidum polysaccharide by combining microwave heating action and ultrasonic cavitation and extracting the alkali-soluble polysaccharide in the ganoderma lucidum by utilizing the alkali extraction method, thereby reducing the loss of the ganoderma lucidum polysaccharide; and the prepared ganoderma lucidum polysaccharide has the characteristics of high production yield, strong immunological activity and the like.
Description
Technical field
The invention provides a kind of preparation method of ganoderma polysaccharide of high yield.
Background technology
Glossy ganoderma is the general name of red sesame of Basidiomycetes polyporaceae (Polyporaceae) Ganoderma (Ganoderma) fungi (G.lucidum) and purple sesame (G.japonicum), claim " Ganoderma lucidum seu Japonicum ", " celestial grass ", " auspicious grass " again, beginning is stated from " Shennong Bencaojing ", has strong, the effect such as strengthen the body resistance to consolidate the constitution, promote longevity of invigorating the spleen and replenishing QI, enriching yin.Ganoderan is one of main functional component of glossy ganoderma, and pharmacological testing shows that ganoderan has multiple pharmacological effect, as antitumor, hypoglycemic, immunomodulatory, anti-oxidant, antiviral etc.The hot water extraction is to extract ganoderan method commonly used.But there is long, problem such as temperature is high, yield is low of extraction time in the traditional hot water extract method, for overcoming the deficiency of traditional hot water extraction method, in recent years, has occurred ultrasonic or new extractive technique and method such as microwave-assisted extraction, ultra-high voltage, enzyme process.
Jin Danhong etc. have compared the influence to the ganoderan yield of Soxhlet, ultrasonic wave, three kinds of extracting method of microwave, and the result shows that microwave extraction method polysaccharide yield is the highest, be 0.868%.(Different Extraction Method is to the influence of ganoderan extraction yield, Chinese pharmacist, 2008,11 (5): 551-552).Du Bing etc. have carried out the technical study of ultra-high voltage extraction Ganoderma spore powder polysaccharide, polysaccharide yield is 2.763% under optimum extraction conditions, and conventional water extraction method polysaccharide yield only is the 2.014% (technical study of ultra-high voltage extraction Ganoderma spore powder polysaccharide, modern food science and technology, 2009,25 (5): 420-422,430).Wu Yingming etc. studies show that ultrasonication method and very low temperature freeze-thaw method have practical value in conjunction with extracting the Ganoderma spore powder polysaccharide, its yield can reach the 1.786% (influence that ultrasonic wave-very low temperature freeze thawing treatment is extracted the Ganoderma spore powder polysaccharide, edible fungi of china, 2008,27 (3): 34-35).Zhou Ming etc. adopt extracting Ganoderma lucidum polysaccharide by ultrasonic wave-microwave combination, and its yield can reach 1.49%, and (ultrasonic-microwave synergistic extraction is to the influence of ganoderan extraction yield, hubei agricultural science, 2009,48 (7): 1727-1729.).Inventions " enzymolysis prepares the method for ganoderan " (200410019093.9) such as China National Academy of Food ﹠ Fermentation Industries Cai Yong peak, the mixed enzyme of utilizing mycelium self enzyme system and adding ties up under the suitable condition carries out enzymolysis to the Ganoderma fermented liquid, behind the enzymolysis in the ganoderma lucidum crude polysaccharide product ganoderma polyoses content be 35-60%.Tianjin Forestry and Fruit Tree Inst.'s Zhang Zhijun etc. inventions " preparation method of ganoderma polysaccharide " (200510014592.3), the main ganoderan product that Ganoderma sporophore is obtained through method such as pre-treatment, micro-filtration, subzero fractionation, membrane sepn, membrane concentration, concentrating under reduced pressure, drying under reduced pressure that adopts.The Hu Qiuhui of Agricultural University Of Nanjing etc. invent " a kind of high efficiency preparation method of ganoderan " (200810156039.7), adopt ultrasonic wave extraction, polysaccharide yield is 20.79 ± 0.11mg/g, purity has reached 47.88%, compare with traditional hot water lixiviate, time has shortened 96%, and yield has improved 28%.Hubei Province Academy of Agricultural Sciences, the processing of agricultural products and Nuclear Agricultural Research Institute Zhou Ming etc. invent " a kind of method of ultrasonic wave microwave synergistic extraction ganoderan " (200810048683.2), has the bonding force that alternative extraction solvent kind is many, the target extract is in extensive range, reduce target compound and sample matrices, quicken the process that target compound enters solvent phase from solid phase, and characteristics such as quantity of sample handling is big.Wu Mei etc. have carried out the research that alkali is carried the caustic solubility lucidum mycelium polysaccharide, wherein adopt 0.5mol/L NaOH dissolving alcohol to analyse, and sugared yield is that 1.3% (alkali is carried the research of caustic solubility lucidum mycelium polysaccharide method, Yunnan University's journal, 1999,21 (2): 160-161).
From present result of study as can be seen, carry out the extraction of water-soluble ganoderan merely, its extraction yield has only about 3%, also contain other polysaccharide of part in the glossy ganoderma residue after the extraction, as the caustic solubility protein-polysaccharide, glycosaminoglycan is if directly abandon, both caused the wasting of resources, again environment had been polluted.Carry out the extraction of caustic solubility ganoderan merely, owing to there be not the recovery of consideration to water-soluble ganoderan, its extraction yield is also very limited.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of ganoderma polysaccharide of high yield, this method is utilized microwave heating action and the cavitation effect of ultrasonic waves extraction ganoderan that mutually combines, utilize the caustic solubility polysaccharide in the alkaline process extraction method extraction glossy ganoderma again, reduce the loss of ganoderan, and the ganoderan for preparing have characteristics such as production yield height and immunocompetence are strong.
The objective of the invention is to be achieved through the following technical solutions: a kind of high yield preparation method of ganoderma polysaccharide, it is characterized in that, earlier Ganoderma sporophore is ground into Ganoderma powder, adopt microwave-assisted hot water extraction method, ultrasonic wave auxiliary heat water extraction method to extract then successively, the extracting solution of gained obtains water-soluble ganoderan after concentrated, impurity elimination, alcohol precipitation and drying; Adopt alkali extraction method that the glossy ganoderma residue is extracted again, the extracting solution of gained through neutralization, dialysis, concentrate, obtain the caustic solubility ganoderan after impurity elimination, alcohol precipitation and the drying treatment.
Concrete steps of the present invention are as follows:
(1) is that raw material carries out pulverization process with the Ganoderma sporophore, obtains Ganoderma powder;
(2) Ganoderma powder is 1 with water by solid-liquid ratio: 15-1: after 25 (g/ml) mix, carry out the microwave-assisted hot water extraction earlier, carry out ultrasonic wave auxiliary heat water extraction then, centrifugation gets supernatant liquor and glossy ganoderma residue, and supernatant liquor obtains water-soluble ganoderan after concentrated, impurity elimination, alcohol precipitation and drying;
(3) by solid-liquid ratio 1: 10-1: add alkali lye in the glossy ganoderma residue of 20 (g/ml) in step (2) and carry out the alkaline process extraction, the extracting solution centrifugation gets supernatant liquor and glossy ganoderma residue, supernatant liquor through neutralization, dialysis, concentrate, obtain the caustic solubility ganoderan after impurity elimination, alcohol precipitation and the drying.
Adopt the micronizing method that Ganoderma sporophore is pulverized in the step of the present invention (1), the granularity 100-200 order of the Ganoderma powder of gained, the Ganoderma powder moisture content is 13-15%.Adopt the micronizing method to pulverize Ganoderma sporophore, the glossy ganoderma fiber is shortened, be more conducive to the polysaccharide stripping.
The condition that microwave-assisted described in the step of the present invention (2) extracts is: microwave power is 300-500W, and extraction time is 3-5min.The condition of described ultrasonic-assisted extraction is: ultrasonic temperature 40-60 ℃, power is 500-600W, and ultrasonic time is 30-50min, and ultrasonic 5 seconds stop 5 seconds (being dutycycle 5S/5S), and ultrasonic total time is 60-100min.
The condition that alkaline process extracts in the step of the present invention (3) is: extract 50-70 ℃ of temperature, time 60-100min.Alkali lye is NaOH solution or KOH solution in the described step (3), and its mass percent concentration is 4%~6%.
Neutralization in the described step (3), dialysis, impurity elimination, concrete operations concentrated and drying treatment are:
Supernatant liquor is transferred about PH to 7 with concentrated hydrochloric acid, and concentrating under reduced pressure is to small volume, but puts in the dialysis tubing of molecular weight cut-off>7000, with deionized water dialysis 3-4 days, till specific conductivity does not change, take out dialyzate, in 50-55 ℃ of concentrating under reduced pressure, add ethanol and transfer to that to contain alcohol amount be 80%, standing over night, centrifuged deposit are used dehydrated alcohol, washing with acetone, lyophilize successively, weigh, promptly get the caustic solubility ganoderan.
The present invention can do following improvement: before the microwave-assisted extraction of carrying out step (2) and ultrasonic-assisted extraction Ganoderma powder is carried out impurity elimination and handle, its concrete operations are: add ethanol in Ganoderma powder, Ganoderma powder and alcoholic acid solid-liquid ratio are 1: 15-1: 20 (g/ml), reflux 2 times at 70-80 ℃, each 2~3h, centrifugal removal supernatant liquor, the precipitation drying obtains getting rid of the Ganoderma powder of pure soluble components, part monose, oligose and lipid material.Handle endogenous enzyme that can the passivation glossy ganoderma and remove pure soluble components, part monose, oligose and lipid material in the Ganoderma powder through impurity elimination, with the purity of the ganoderan that improves gained.
Described centrifugal condition in step among the present invention (2) and the step (3) is: under the 3500-4500r/min condition centrifugal 10-12 minute.Described alcoholic acid mass percent concentration is 90~95%.
Ganoderma sporophore of the present invention adopts red ganoderma sporophore or Ganoderma sinense Zhao, Xu et Zhang sporophore.
The present invention's beneficial effect compared with prior art:
(1) the present invention adopts the micronizing method to pulverize Ganoderma sporophore, the glossy ganoderma fiber is shortened, be beneficial to the polysaccharide stripping, utilize microwave heating action and the cavitation effect of ultrasonic waves extraction ganoderan that mutually combines again, solve that the product yield that existing hot water extraction ganoderan and single extracting method exist is low, extraction time problem such as length, temperature height, and solve the problems such as loss that simple alkaline process extracts does not have consideration that its water-soluble ganoderan is reclaimed or extracts and causes.And the water-soluble ganoderan of gained and caustic solubility ganoderan all keep the activity (referring to table 1 and table 2) of higher enhancing immune function of mice.
Table 1 polysaccharide is to the influence of mouse delayed allergy, serum hemolysin generation and NK cell killing activity
Annotate:
1., 2.,Expression is compared P<0.05 with (1), (2) group respectively
Table 2 polysaccharide is to the influence of hemolysin test mouse thymus index, index and spleen index
Annotate:
1., 2.,Expression is compared P<0.05 with (1), (2) group respectively
With reference to the standard that Ministry of Health of the People's Republic of China's protective foods check and evaluation technique standard (version in 2003) are judged about strengthening immunity functional evaluation test-results, has the effect of strengthening immunity according to this result of study decidable ganoderan.
(2) the present invention compares with conventional hot water extraction (conventional 10 orders of pulverizing), and the extraction temperature is low, the time short and yield can improve 3.43 times, if compare with the conventional hot water extraction of glossy ganoderma sample (micronizing 150 orders) of equal order number, extraction yield can improve 2.47 times.Comparing result sees Table 3.
Table 3 different process is to the influence of the yield of water-soluble ganoderan
(3) the present invention compares with conventional hot water extraction (conventional 10 orders of pulverizing), the extraction temperature is low, the time short and yield can improve 3.43 times, if compare with the conventional hot water extraction of Ganoderma powder sample (micronizing 150 orders) of equal order number, extraction yield can improve 2.47 times.Comparing result sees Table 4.
Table 4 different process is to the influence of the yield of caustic solubility ganoderan
Remarks: other extraction conditions: solid-liquid ratio 1: 20,50 ℃ of temperature of extraction, NaOH concentration of lye 5% unanimity.
(4) the present invention carries out the ethanol impurity elimination and handles before the extraction of carrying out ganoderan, passivation glossy ganoderma endogenous enzyme and remove pure soluble components, part monose, oligose and lipid material in the Ganoderma powder, with the purity of the ganoderan that improves gained.
Description of drawings
Fig. 1 is the process flow sheet of ganoderan high efficiency preparation method of the present invention.
Embodiment:
Embodiment 1:
(1) be that raw material carries out the micronizing processing with the red ganoderma sporophore, obtain the about 150 hot eyes Ganoderma powder of granularity, the red ganoderma powder with 90% ethanol 80 ℃ of reflow treatment 2 times, each 3h, red ganoderma powder and alcoholic acid solid-liquid ratio 1: 15 (g/ml), centrifugal then, remove supernatant liquor, gained be deposited in the Ganoderma powder that 60 ℃ of oven dryings obtained getting rid of pure soluble components, part monose, oligose and lipid material in 5 hours.
(2) accurately take by weighing 5g red ganoderma powder, wherein the moisture content of red ganoderma powder is 13.26%, as solvent, the solid-liquid ratio of red ganoderma powder and water is 1: 25 (g/ml) with water, adopts the microwave-assisted hot water extraction water-soluble ganoderan of ultrasonic wave auxiliary heat water extraction then earlier.Wherein, the microwave-assisted extraction conditions is: microwave power 300W, extraction time 3min; The ultrasonic-assisted extraction condition is: ultrasonic power 500W, extract 40 ℃ of temperature, and extraction time is 30min, surpasses 5 seconds and stopped 5 seconds, i.e. dutycycle 5S/5S, extraction total time is 60min.With extracting solution under the 4500r/min condition centrifugal 11 minutes, collect supernatant liquor, lyophilize obtains water-soluble ganoderan crude product, and measuring water-soluble polysaccharide content by the sulfuric acid phynol method is 5.12%.To precipitate then with 60 ℃ of oven dryings 5 hours, the red ganoderma residue be used to carry out alkaline process and extracts.
(3) the red ganoderma residue to gained in the step (2) carries out alkaline process extraction caustic solubility ganoderan, extraction conditions is: 50 ℃ of alkali temperature raising degree, alkali is carried time 70min, NaOH solution quality percentage concentration is 4%, the liquid material of red ganoderma residue and NaOH solution was than 1: 10 (g/ml), with the extracting solution of gained under the 4000r/min condition centrifugal 10 minutes, collect supernatant liquor, transfer about PH to 7 with hydrochloric acid, concentrating under reduced pressure was to small volume, but puts in the dialysis tubing of molecular weight cut-off>7000, with deionized water dialysis 3 days, till specific conductivity does not change, take out dialyzate,, add mass percent concentration and be 95% ethanol and transfer to that to contain the alcohol amount in the solution be 80% in 50 ℃ of concentrating under reduced pressure, standing over night, centrifugal then, abandoning supernatant, the precipitation of gained is used dehydrated alcohol successively, washing with acetone, lyophilize obtains caustic solubility ganoderan crude product, and measuring the caustic solubility polysaccharide content by the sulfuric acid phynol method is 9.83%.
Embodiment 2:
(1) be that raw material carries out the micronizing processing with the red ganoderma sporophore, obtain the about 200 hot eyes Ganoderma powder of granularity, the red ganoderma powder with 95% ethanol 70 ℃ of reflow treatment 2 times, each 3h, red ganoderma powder and alcoholic acid solid-liquid ratio 1: 20 (g/ml), centrifugal then, remove supernatant liquor, gained be deposited in the Ganoderma powder that 60 ℃ of oven dryings obtained getting rid of pure soluble components, part monose, oligose and lipid material in 5 hours.
(2) accurately take by weighing 5g red ganoderma powder, wherein the moisture content of red ganoderma powder is 13.26%, as solvent, the solid-liquid ratio of red ganoderma powder and water is 1: 20 (g/ml) with water, adopts the water-soluble ganoderan of the ultrasonic then auxiliary heat water extraction of microwave-assisted hot water extraction earlier.Wherein, the microwave extraction condition is: microwave power 400W, extraction time 4min; The ultrasonic-assisted extraction condition is: ultrasonic power 550W, extract 55 ℃ of temperature, and extraction time 35min surpasses 5 seconds and stopped 5 seconds, i.e. dutycycle 5S/5S, extraction total time is 70min.With the extracting solution of gained under the 4000r/min condition centrifugal 10 minutes, collect supernatant liquor, lyophilize obtains water-soluble ganoderan crude product, and measuring water-soluble polysaccharide content by the sulfuric acid phynol method is 5.46%.To precipitate then with 60 ℃ of oven dryings 5 hours, the glossy ganoderma residue be used to carry out alkaline process and extracts.
(3) the red ganoderma residue to gained in the step (2) carries out alkaline process extraction caustic solubility ganoderan, extraction conditions is: 60 ℃ of alkali temperature raising degree, alkali is carried time 60min, the mass percent concentration of NaOH solution is 5%, the liquid material was than 1: 20 (g/ml), with said extracted liquid under the 4000r/min condition centrifugal 10 minutes, collect supernatant liquor, transfer about PH to 7 with hydrochloric acid, concentrating under reduced pressure is to small volume, put can the dialysis tubing of molecular weight cut-off>7000 in, with deionized water dialysis 4 days, till specific conductivity does not change, take out dialyzate, in 53 ℃ of concentrating under reduced pressure, add mass percent concentration and be 95% ethanol and transfer to that to contain the alcohol amount be 80%, standing over night, centrifuged deposit is used dehydrated alcohol successively, washing with acetone, lyophilize obtains caustic solubility ganoderan crude product, and measuring the caustic solubility polysaccharide content by the sulfuric acid phynol method is 10.74%.
Embodiment 3:
(1) be that raw material carries out the micronizing processing with the red ganoderma sporophore, obtain the about 100 hot eyes Ganoderma powder of granularity, the red ganoderma powder with 95% ethanol 75 ℃ of reflow treatment 2 times, each 3h, red ganoderma powder and alcoholic acid solid-liquid ratio 1: 18 (g/ml), centrifugal then, remove supernatant liquor, gained be deposited in the Ganoderma powder that 60 ℃ of oven dryings obtained getting rid of pure soluble components, part monose, oligose and lipid material in 5 hours.
(2) accurately take by weighing 5g red ganoderma powder, wherein the moisture content of red ganoderma powder is 13.26%, as solvent, the solid-liquid ratio of red ganoderma powder and water is 1: 15 (g/ml) with water, adopts the water-soluble ganoderan of the ultrasonic then auxiliary heat water extraction of microwave-assisted hot water extraction earlier.Wherein, the microwave extraction condition is: microwave power 500W, extraction time 5min; The ultrasonic-assisted extraction condition is: ultrasonic power 600W, extract 60 ℃ of temperature, and extraction time 40min surpasses 5 seconds and stopped 5 seconds, i.e. dutycycle 5S/5S, extraction total time is 80min.With the extracting solution of gained under the 3000r/min condition centrifugal 12 minutes, collect supernatant liquor, lyophilize obtains water-soluble ganoderan crude product, and measuring water-soluble polysaccharide content by the sulfuric acid phynol method is 5.31%.To precipitate then with 60 ℃ of oven dryings 5 hours, the glossy ganoderma residue be used to carry out alkaline process and extracts.
(3) the red ganoderma residue to gained in the step (2) carries out alkaline process extraction caustic solubility ganoderan, extraction conditions is: 70 ℃ of alkali temperature raising degree, alkali is carried time 80min, the mass percent concentration of NaOH solution is 5.5%, the liquid material was than 1: 15 (g/ml), with said extracted liquid under the 4000r/min condition centrifugal 15 minutes, collect supernatant liquor, transfer about PH to 7 with hydrochloric acid, concentrating under reduced pressure is to small volume, put can the dialysis tubing of molecular weight cut-off>7000 in, with deionized water dialysis 4 days, till specific conductivity does not change, take out dialyzate, in 55 ℃ of concentrating under reduced pressure, add mass percent concentration and be 95% ethanol and transfer to that to contain the alcohol amount be 80%, standing over night, centrifuged deposit is used dehydrated alcohol successively, washing with acetone, lyophilize obtains caustic solubility ganoderan crude product, and measuring the caustic solubility polysaccharide content by the sulfuric acid phynol method is 10.26%.
Claims (10)
1. the preparation method of ganoderma polysaccharide of a high yield, it is characterized in that, earlier Ganoderma sporophore is ground into 100-200 purpose Ganoderma powder, adopt microwave-assisted hot water extraction method, ultrasonic wave auxiliary heat water extraction method to extract then successively, the extracting solution of gained is through concentrating, impurity elimination, concentrating, obtain water-soluble ganoderan after alcohol precipitation and the drying; Adopt alkali extraction method that the glossy ganoderma residue is extracted again, the extracting solution of gained through neutralization, dialysis, concentrate, obtain the caustic solubility ganoderan after impurity elimination, alcohol precipitation and the drying treatment.
2. preparation method of ganoderma polysaccharide according to claim 1 is characterized in that it may further comprise the steps:
(1) is that raw material carries out pulverization process with the Ganoderma sporophore, obtains 100-200 purpose Ganoderma powder;
(2) Ganoderma powder is 1 with water by solid-liquid ratio: 15-1: after 25g/ml mixes, carry out the microwave-assisted hot water extraction earlier, carry out ultrasonic wave auxiliary heat water extraction then, centrifugation gets supernatant liquor and glossy ganoderma residue, and supernatant liquor obtains water-soluble ganoderan after concentrated, impurity elimination, alcohol precipitation and drying;
(3) carry out the alkaline process extraction by adding alkali lye in the glossy ganoderma residue of solid-liquid ratio 1: 10-1: 20g/ml in step (2), the extracting solution centrifugation gets supernatant liquor and glossy ganoderma residue, supernatant liquor through neutralization, dialysis, concentrate, obtain the caustic solubility ganoderan after impurity elimination, alcohol precipitation and the drying.
3. preparation method of ganoderma polysaccharide according to claim 2 is characterized in that, adopts the micronizing method that Ganoderma sporophore is pulverized in the described step (1), and the Ganoderma powder moisture content of gained is 13-15%.
4. preparation method of ganoderma polysaccharide according to claim 2 is characterized in that, the condition that the microwave-assisted described in the described step (2) extracts is: microwave power is 300-500W, and extraction time is 3-5 minute; The condition of described ultrasonic-assisted extraction is: ultrasonic temperature 40-60 ℃, power is 500-600W, and ultrasonic time is 30-50min, and ultrasonic 5 seconds stopped 5 seconds, and ultrasonic total time is 60-100min.
5. preparation method of ganoderma polysaccharide according to claim 2 is characterized in that, the condition that alkaline process extracts in the described step (3) is: extract 50-70 ℃ of temperature, time 60-100min; Alkali lye in the described step (3) is NaOH solution or KOH solution, and its mass percent concentration is 4-6%.
6. preparation method of ganoderma polysaccharide according to claim 2 is characterized in that, the neutralization in the described step (3), dialysis, impurity elimination, concrete operations concentrated and drying treatment are:
Supernatant liquor is transferred PH to 7 with concentrated hydrochloric acid, and concentrating under reduced pressure is to small volume, but puts in the dialysis tubing of molecular weight cut-off>7000, with deionized water dialysis 3-4 days, till specific conductivity does not change, take out dialyzate, in 50-55 ℃ of concentrating under reduced pressure, add ethanol and transfer to that to contain alcohol amount be 80%, standing over night, centrifuged deposit are used dehydrated alcohol, washing with acetone, lyophilize successively, weigh, promptly get the caustic solubility ganoderan.
7. according to claim 2 or 3 or 4 or 5 or 6 described preparation method of ganoderma polysaccharide, it is characterized in that, before the microwave-assisted extraction of carrying out described step (2) and ultrasonic-assisted extraction Ganoderma powder being carried out impurity elimination handles, its concrete operations are: add ethanol in Ganoderma powder, Ganoderma powder and alcoholic acid solid-liquid ratio are 1: 15-1: 20g/ml, reflux 2 times at 70-80 ℃, each 2-3h, centrifugal removal supernatant liquor, the precipitation drying obtains getting rid of the Ganoderma powder of pure soluble components, part monose, oligose and lipid material.
8. preparation method of ganoderma polysaccharide according to claim 7 is characterized in that, the described centrifugal condition in the described step (2) is: centrifugal rotational speed 3500-4500r/min, centrifugation time 10-12 minute.
9. preparation method of ganoderma polysaccharide according to claim 7 is characterized in that, described alcoholic acid mass percent concentration is 90~95%.
10. preparation method of ganoderma polysaccharide according to claim 1 and 2 is characterized in that, described Ganoderma sporophore is red ganoderma sporophore or Ganoderma sinense Zhao, Xu et Zhang sporophore.
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