CN107868138A - A kind of preparation method of GL-B - Google Patents
A kind of preparation method of GL-B Download PDFInfo
- Publication number
- CN107868138A CN107868138A CN201711319196.0A CN201711319196A CN107868138A CN 107868138 A CN107868138 A CN 107868138A CN 201711319196 A CN201711319196 A CN 201711319196A CN 107868138 A CN107868138 A CN 107868138A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- crushing
- ganoderma lucidum
- preparation
- hot water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of GL-B, comprise the following steps：The ganoderma lucidum after cleaning-drying is subjected to coarse crushing first, ethanol is added in the ganoderma lucidum Jing Guo coarse crushing and soaks 4 6h, and is dried；Ganoderma lucidum is subjected to mechanical crushing, and hot water extraction is used for multiple times after crushing, is mixed after hot water is concentrated with ethanol, extracts clear liquid after centrifugation, and ethanol evaporation obtains sediment again by the 2h of ultrasonic grind 1 after mechanical crushing；Deionized water is added into sediment, and adds the processing of protease constant temperature thereto, filtering obtains purification filtrate, then adds absolute ethyl alcohol dissolving thereto, strengthens heat in ethanol solution upon dissolution, and ethanol evaporation obtains sediment after filtration；The efficiency of extraction greatly improves, and work(is retainable more intact, in addition, process is simple, energy consumption is low, is easy to the automation mechanized operation of whole process.
Description
Technical field
The present invention relates to purification technique field, specially a kind of preparation method of GL-B.
Background technology
GL-B is the secondary metabolite of Polyporaceae Ganoderma fungal mycelium, is present in the bacterium of Ganoderma fungi
In filament and fructification.Conventional preparation method is：Ganoderma lucidum fruitbody, which crushes, adds the fermentation of a small amount of culture medium, after fermentation raw material from
The heart, the concentration of supernatant cryogenic vacuum, dialysis, ethanol precipitation, acetone washing, removing protein, spray drying, it is eventually adding 10% water extraction
Dry powder blend forms.
But in the above method, GL-B impurity content height is obtained, and purity is low, it is impossible to meet actual purity need
Ask.In order to solve the above problems, in existing technical scheme, as Application No. 201010145080.1 announce it is a kind of it is high must
The preparation method of the GL-B of rate, being be combined with each other using the cavitation of microwave heating action and ultrasonic wave, it is more to extract ganoderma lucidum
Sugar, the alkali-soluble polysaccharide in alkalinity extraction method extraction ganoderma lucidum is recycled, reduces the loss of GL-B, and the spirit being prepared
Sesame polysaccharide has the characteristics that production yield is high and immunocompetence is strong.The alkaline matter that whole process adds easily becomes new miscellaneous
Matter, inconvenience are thoroughly removed, and flow is complicated, is not easy to the pile line operation automated, and alkaline components
It is easily destroyed the active component of GL-B.
The content of the invention
In order to overcome the shortcomings of prior art, the present invention provides a kind of preparation method of GL-B, the effect of extraction
Rate greatly improves, and work(is retainable more intact, in addition, process is simple, energy consumption is low, is easy to the automation mechanized operation of whole process,
It can effectively solve the problem that the problem of background technology proposes.
The technical solution adopted for the present invention to solve the technical problems is：
A kind of preparation method of GL-B, comprises the following steps：
S100, the pretreatment of ganoderma lucidum, the ganoderma lucidum after cleaning-drying is subjected to coarse crushing first, by coarse crushing
Ethanol immersion 4-6h is added in ganoderma lucidum, and is dried；
Ganoderma lucidum is carried out mechanical crushing, and pass through again after mechanical crushing by S200, ganoderma lucidum crushing with separating for the first time
Ultrasonic grind 1-2h, hot water extraction is used for multiple times after crushing, is mixed with ethanol after hot water is concentrated, extracted after centrifugation
Clear liquid, and ethanol evaporation obtains sediment；
S300, GL-B purification, deionized water is added into sediment, and added thereto at protease constant temperature
Reason, filtering obtain purification filtrate, then add absolute ethyl alcohol dissolving thereto, strengthen in ethanol solution upon dissolution
Heat, and ethanol evaporation obtains sediment after filtration.
As a kind of preferable technical scheme of the present invention, in the step s 100, the ganoderma lucidum moisture content after cleaning-drying exists
More than 60%, and the ethanol mass fraction of immersion is 70-80%.
As a kind of preferable technical scheme of the present invention, 60 mesh sieves are crossed after mechanical crushing, and between ultrasonic grind use
The reciprocal mode of gap type, the work of single ultrasonic grind 20-30s, intermittent time 10-20s.
As a kind of preferable technical scheme of the present invention, 80-90 DEG C of hot water is used in step s 200, after ultrasonic grind
Extracted, and 15-30min high speed centrifugation carried out under the rotating speed after hot water concentration in 10000-12000r/min,
Remove sediment.
As a kind of preferable technical scheme of the present invention, in step S300, the specific processing method of protease is：
First, protease is added；
Secondly maintaining operation temperature, the acid-base value for adjusting solution is 6.5-7.6, and maintains above-mentioned state 1- at 35-40 DEG C
2h, stirred during above-mentioned condition is maintained with 360r/min low speed rotation；
Finally, maintain identical state to be filtered, remove clear liquid.
As a kind of preferable technical scheme of the present invention, in step S300, strengthen 90-100 DEG C of the temperature of heat, and tie up
The time for holding the temperature is no more than 45min, is filtered in this condition, the temperature of ethanol evaporation maintains 50-60 after filtering
℃。
Compared with prior art, the beneficial effects of the invention are as follows：
The method that the present invention is combined by mechanical crushing and ultrasonic grind so that ganoderma lucidum somatic cells are all broken,
Be advantageous to the extraction of GL-B isoreactivity composition, avoid to extract by force and damage and lose to caused by active component, and
So that the efficiency of broken extraction greatly improves, work(is retainable more intact, is easy to the automation mechanized operation of whole process, in addition,
Process is simple, and energy consumption is low, and the time is short, can preferably keep the natural activity of GL-B, is adapted to specific industrialized production.
Brief description of the drawings
Fig. 1 is the schematic flow sheet of the present invention；
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
As shown in figure 1, the invention provides a kind of preparation method of GL-B, comprise the following steps：
Step S100, the pretreatment of ganoderma lucidum, the ganoderma lucidum after cleaning-drying is subjected to coarse crushing first, by coarse powder
Ethanol immersion 4-6h is added in broken ganoderma lucidum, and is dried.
In the step s 100, the ganoderma lucidum moisture content after cleaning-drying is more than 60%, and the ethanol mass fraction of immersion
For 70-80%.
In the present embodiment, the moisture content for keeping ganoderma lucidum be in order to be pre-processed in the later stage in, by the immersion of ethanol, have
Beneficial to the lipid material removed in wet thallus ganoderma lucidum, while glycosidase is lost activity, prevent the degraded of polysaccharide, be advantageous to subtracting
The degraded of polysaccharide can also be prevented while few impurity, preserves the polysaccharide active components of ganoderma lucidum as far as possible.
And carry out coarse crushing in order to improve the contact surface area of immersion, improve the efficiency of processing so that immersion more
Effectively, further, coarse crushing can also prevent the phenomenon of dust or other airborne dusts in dry ganoderma lucidum thalline.
Step S200, ganoderma lucidum is carried out mechanical crushing by the crushing of ganoderma lucidum with separating for the first time, and after mechanical crushing again
By ultrasonic grind 1-2h, hot water extraction is used for multiple times after crushing, is mixed after hot water is concentrated with ethanol, after centrifugation
Clear liquid is extracted, and ethanol evaporation obtains sediment.
Wherein, 60 mesh sieves are crossed after mechanical crushing, use fine crushing in wet thallus after steeping, it is ensured that after crushing
Ganoderma lucidum can reach defined granular size, be easy to subsequent treatment, improve the efficiency of subsequent treatment.
, it is necessary to further illustrate the work detail parameters of ultrasonic wave in above-mentioned：Ultrasonic wave cumulative working frequency 60-
100KHz, ultrasonic power 2000-3000W, high-frequency and high-power ultrasonic grind can above carry in certain degree
The efficiency that height crushes, but also enable to the moisture of ganoderma lucidum body interior to produce resonance so that the effect of crushing is more preferable, favorably
In the extraction of GL-B.
As preferred embodiment, ultrasonic grind is by the way of clearance-type is reciprocal, the work of single ultrasonic grind
20-30s, intermittent time 10-20s, this is due to the reciprocating manner for producing batch (-type), and machine can be produced during crushing
Tool vibrates so that change in location can occur in mechanical oscillation for REISH, and the change in location during this gap, just
The efficiency of vibration can be improved in ultrasonic activation.
In step s 200, extracted after ultrasonic grind with 80-90 DEG C of hot water, and hot water concentration after
15-30min high speed centrifugation is carried out under 10000-12000r/min rotating speed, removes sediment.
Hot water extraction effect be raising powder dissolving, the solution state that all the components are become as far as possible, and
And it is unstable in the material after ultrasonic grind, in of short duration colloidal state, be convenient for after solution is turned to from
The heart extracts.
In this course it should also be noted that, also containing substantial amounts of egg in the GL-B handled by above-mentioned steps
The small molecule composition such as white matter, pigment and oligosaccharide, and the presence of these small molecule compositions so that the water solubility of GL-B is significantly
Improve, and in order to reduce the aqueous solution of GL-B, it is necessary to the temperature and pH value of solution are adjusted, and goes the removal of impurity as far as possible
Composition, it is easy to follow-up purifying.
Step S300, the purification of GL-B, deionized water is added into sediment, and it is permanent to add protease thereto
Temperature processing, filter and obtain purification filtrate, then add absolute ethyl alcohol dissolving thereto, add in ethanol solution upon dissolution
Heat-flash, and the ethanol evaporated after filtration in clear liquid obtains sediment.
In step S300, the specific processing method of protease is：
First, protease is added；
Secondly maintaining operation temperature, the acid-base value for adjusting solution is 6.5-7.6, and maintains above-mentioned state 1- at 35-40 DEG C
2h, stirred during above-mentioned condition is maintained with 360r/min low speed rotation；
Finally, maintain identical state to be filtered, remove clear liquid.
In above-mentioned filtering, it is necessary to it is specifically intended that because GL-B is dissolved in hot water, therefore in filtering
When maintain the acid-base value of solution, and the temperature of solution then needs to carry out selectivity, adaptability tune according to required state
It is whole, to be filtered when GL-B dissolution degree is minimum, the active component of GL-B is kept as far as possible, is prevented
Only in processing procedure active ingredient scatter and disappear.
Add the handling principle of protease：Be firstly added protease effect be to be contained in protein therein,
The protein ingredient of pigment and oligosaccharide is all hydrolyzed, and the process hydrolyzed needs to keep the activity of protease, therefore will
Appropriate change working condition keeps the activity of protease；And after hydrolysis is completed, protease is again by as new miscellaneous
Matter, therefore inactivated by the living features of protease by strengthening heat, solid sediment is become, by filtering off
Remove.
In addition, in step S300, strengthen 90-100 DEG C of the temperature of heat, and maintain the time of the temperature to be no more than
45min, filtered in this condition, the temperature of ethanol evaporation maintains 50-60 DEG C after filtering.
Specifically, in the present embodiment, because ethanol is separated by evaporation, therefore, by rational
Recycle, ethanol is not have lossy in whole technique, can be recycled always.
In summary, the main characteristic of the invention lies in that：
(1) method being combined by mechanical crushing and ultrasonic grind so that ganoderma lucidum somatic cells are all broken, favorably
In the extraction of GL-B isoreactivity composition, avoid to extract by force and damage and lose to caused by active component, and cause
The efficiency of broken extraction greatly improves, and work(is retainable more intact, is easy to the automation mechanized operation of whole process；
(2) process is simple, and energy consumption is low, and the time is short, can preferably keep the natural activity of GL-B, is adapted to specific
Industrialized production.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Claims (6)
1. a kind of preparation method of GL-B, it is characterised in that comprise the following steps：
S100, the pretreatment of ganoderma lucidum, the ganoderma lucidum after cleaning-drying is subjected to coarse crushing first, in the ganoderma lucidum Jing Guo coarse crushing
Middle addition ethanol immersion 4-6h, and dry；
Ganoderma lucidum is carried out mechanical crushing, and pass through ultrasound again after mechanical crushing by S200, ganoderma lucidum crushing with separating for the first time
Ripple crushes 1-2h, and hot water extraction is used for multiple times after crushing, is mixed after hot water is concentrated with ethanol, extraction is clear after centrifugation
Liquid, and ethanol evaporation obtains sediment；
S300, GL-B purification, deionized water is added into sediment, and adds the processing of protease constant temperature thereto,
Filtering obtains purification filtrate, then adds absolute ethyl alcohol dissolving thereto, strengthens heat in ethanol solution upon dissolution, and
Ethanol evaporation obtains sediment after filtration.
A kind of 2. preparation method of GL-B according to claim 1, it is characterised in that：In the step s 100, clean
Dried ganoderma lucidum moisture content is more than 60%, and the ethanol mass fraction of immersion is 70-80%.
A kind of 3. preparation method of GL-B according to claim 1, it is characterised in that：60 mesh are crossed after mechanical crushing
Sieve, and ultrasonic grind is using clearance-type is reciprocal by the way of, single ultrasonic grind work 20-30s, intermittent time 10-
20s。
A kind of 4. preparation method of GL-B according to claim 1, it is characterised in that：In step s 200, it is ultrasonic
Ripple is extracted after crushing with 80-90 DEG C of hot water, and under the rotating speed after hot water concentration in 10000-12000r/min
15-30min high speed centrifugation is carried out, removes sediment.
A kind of 5. preparation method of GL-B according to claim 1, it is characterised in that：In step S300, albumen
The specific processing method of matter enzyme is：
First, protease is added；
Secondly maintaining operation temperature, the acid-base value for adjusting solution is 6.5-7.6, and maintains above-mentioned state 1-2h at 35-40 DEG C,
Maintain to stir with 360r/min low speed rotation during above-mentioned condition；
Finally, maintain identical state to be filtered, remove clear liquid.
A kind of 6. preparation method of GL-B according to claim 1, it is characterised in that：In step S300, strengthen
90-100 DEG C of the temperature of heat, and maintain the time of the temperature to be no more than 45min, filtered, steamed after filtering in this condition
The temperature of hair ethanol maintains 50-60 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711319196.0A CN107868138A (en) | 2017-12-12 | 2017-12-12 | A kind of preparation method of GL-B |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711319196.0A CN107868138A (en) | 2017-12-12 | 2017-12-12 | A kind of preparation method of GL-B |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107868138A true CN107868138A (en) | 2018-04-03 |
Family
ID=61755600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711319196.0A Pending CN107868138A (en) | 2017-12-12 | 2017-12-12 | A kind of preparation method of GL-B |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107868138A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101367881A (en) * | 2008-09-19 | 2009-02-18 | 南京农业大学 | High-efficiency preparation method for glossy ganoderma polysaccharide |
| CN101805414A (en) * | 2010-04-06 | 2010-08-18 | 无限极（中国）有限公司 | Preparation method of ganoderma lucidum polysaccharide with high yield |
-
2017
- 2017-12-12 CN CN201711319196.0A patent/CN107868138A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101367881A (en) * | 2008-09-19 | 2009-02-18 | 南京农业大学 | High-efficiency preparation method for glossy ganoderma polysaccharide |
| CN101805414A (en) * | 2010-04-06 | 2010-08-18 | 无限极（中国）有限公司 | Preparation method of ganoderma lucidum polysaccharide with high yield |
Non-Patent Citations (2)
| Title |
|---|
| 刘新社 等: "《果蔬贮藏与加工技术》", 30 April 2014 * |
| 肖丽霞 等: "响应曲面优化超微粉碎法提取灵芝多糖", 《食品工业科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100999739B (en) | Process for treating straw by vapour explosive and alkaline oxydol oxydizing | |
| CN103583700A (en) | Method capable of improving instant dissolving performance of soybean milk powder | |
| CN111019011B (en) | Method for extracting rice bran polysaccharide | |
| CN105418781A (en) | Ultrasonic extraction method of ganoderan | |
| JP2009125050A (en) | Pretreatment method for enzymatic hydrolysis of herbaceous biomass, ethanol production method using herbaceous biomass as raw material and ethanol production method using palm hollow bunch | |
| CN105542937A (en) | Method for extracting grease from soybeans through deep processing | |
| CN102329382A (en) | Method for extracting rapeseed proteins through ultrasonic-microwave synergy | |
| CN106832027A (en) | The extracting method of gumbo polysaccharide | |
| CN108976304A (en) | A kind of acorn starch and its preparation method and application | |
| CN103724442A (en) | Preparation method for low-protein and high-purity sea cucumber polysaccharide | |
| CN102492054B (en) | Preparation method of soybean episperm pectin gel polysaccharide | |
| CN101074269A (en) | Process for separating and extracting cucurbic active polysaccharide | |
| CN102273578B (en) | Preparation method of rice starch-based fat replacer | |
| CN108148147A (en) | A kind of extracting method of tremella polysaccharides | |
| CN108713601A (en) | A kind of preparation method of low sensitization soy milk powder | |
| CN107868138A (en) | A kind of preparation method of GL-B | |
| CN107383233A (en) | A kind of method for extracting tremella polysaccharides | |
| CN110731409A (en) | method for processing high protein rice flour with broken rice | |
| CN101665534A (en) | Preparation method of concentrated protein of cottonseeds and processing production line thereof | |
| CN108935914A (en) | A kind of full-dry method rice protein powder preparation technique | |
| CN106085251B (en) | A kind of method that gelatin is extracted in the fish-skin from grass carp | |
| CN114164246B (en) | Acer truncatum protein extraction method | |
| CN107903334A (en) | A kind of technique that green alga proteoglycan is extracted from fresh green alga | |
| CN110386959B (en) | Method for extracting and separating rice protein | |
| CN108285499A (en) | A kind of energy-efficient technique for preparing pectin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180403 |
|
| RJ01 | Rejection of invention patent application after publication |